KR101892070B1 - Separation device for Stromal Vascular Fraction Cells - Google Patents

Abstract

The present invention relates to an apparatus for separating epilepsy cells. Liposuction inhalant other than fat from a fat inhalant is separated by filtration and LAF contained in the separated liquid inhalant is precipitated and separated, so that separated LAF precipitate was mixed with blood pretreated with anticoagulant and centrifuged to collect the LAF in buffy coat. Therefore, not only is it possible to fractionate stromal cells from adipose tissue which does not use proteolytic enzymes, but also it is possible to easily remove a large amount of blood cells contained in the LAF separated from the liposuction inhalant.

Description

[0002] Separation device for Stromal Vascular Fraction Cells [

The present invention relates to an apparatus for separating epilepsy cells, and more particularly, to a device for separating LAFs contained in a liquid inhale separated from a liposuction article.

As is well known, it has been found that a large number of regenerative cells including a large number of stem cells are present in the fat tissue, and recently, a method of extracting regenerative cells derived from adipose tissue and equipment therefor have been developed and introduced have.

Regenerative cells including stem cells obtained from adipose tissue are collectively referred to as stromal vascular fraction cells (SVF).

In general, regenerative cells containing stem cells are most commonly obtained after treatment of adipose tissue with proteolytic enzymes (collagenases, etc.), but recently regenerated cells containing stem cells have been obtained in adipose tissue without proteolytic enzyme treatment There are a lot of studies to be done.

There are two ways to obtain SVF without enzymatic treatment. One is the mechanical grinding of adipose tissue, the other is the fractionation from the remaining inhaled liquid except fat in liposuction .

Liposomes include various cells, including fat cells, LAF, and hematopoietic cells.

Liposomes extracted from adipose tissue through liposuction can be separated into fat and liquid fractions except fat.

Liquid inhalation products include body fluid inhalation (LAF, blood, lymph and tissue fluids, etc.) and liposuction (Tumescent solution, saline, etc.).

LAF (Liposuction Aspirated Fluid Fraction Cells) refers to the stromal cells (SVF) contained in body fluid inhaled from liposuction.

However, since the body fluid inhalation contains a considerable amount of blood cells (RBC) and the frequency of LAF is considerably lower than that of blood cells, it is necessary to appropriately remove blood cells in order to use LAF clinically.

Generally, it is the most common method to use a red cell lysis buffer as a method for removing a large amount of blood cells contained in LAF, but the processing is difficult and can be used only by researchers such as research institutes . Therefore, it is very difficult to use hemocyte lysing solution in clinical practice.

Korean Patent Publication No. 10-2006-0037469 (Published Date May 03, 2006) Korean Patent Publication No. 10-2014-0070527 (published on June 10, 2014)

DISCLOSURE OF THE INVENTION An object of the present invention to solve the above problems is to provide an apparatus for separating septal cells for separating LAF contained in a liquid inhale separated from liposuction material.

In order to accomplish the above object, the present invention provides an apparatus for separating epilepsy cells, comprising: a container having an internal space for containing the obtained liposuction material; A filter net installed in the upper portion of the container for filtering the liquid inhalation through the lower portion of the liposuction material; And agitation means which is installed in the inside of the mesh net so as to stir the lipid inhalation inside the mesh net.

Here, the agitating means may include a stirrer suspended in the upper portion of the container in a state of being accommodated in the mesh net, And an agitator provided in the agitator and shaking the container to provide an inertial force when agitating the liposuction material in the mesh.

The upper part of the agitator is suspended and fixed so as to be freely movable on the upper side of the container, and a plurality of arcuate frames forming the outer shape of the lower part may be formed as a line of longitude or ellipsoid.

In addition, the stirring weight may be installed inside the stirring body, or the stirring weight may be suspended from the bottom of the stirring body.

Also, the stirrer may be made of an elastically deformable material. At this time, the elastic force of the agitator may be larger than the load of the agitator.

When the additional motive force generated when the container is gripped by the container to agitate the liposuction material is added to the load of the transilluminator, the stirrer is kept spaced apart from the inner surface of the drawn- So as to perform a free agitating motion to intermittently hit the inner surface of the squeeze net body.

The agitator is elastically deformed so as to induce a jumping operation by the agitator when the container is shaken while holding the container so as to agitate the liposuction material, and impact force that hits discontinuously on the inner surface of the graphene is applied It is preferable to increase the effect of squeezing the squeezed fat through the squeeze net.

The container lid further includes a container cover covering and covering the upper opening of the container, the container cover including an injection pipe penetratingly formed to supply the liposuction material; And an injection pipe opening / closing cap for opening / closing the injection pipe.

In addition, it is preferable that the agitator is fixed to the container lid so as to be freely rotatable.

The apparatus further includes an outlet opening / closing means for opening / closing the outlet of the container, wherein the outlet opening / closing means includes an outlet tube extended to the outlet; And a discharge tube clamp for adjusting the discharge amount of the LAF through the discharge tube.

According to the above-described septal cell separating apparatus of the present invention, the remaining inhaled liquid excluding fat from the liposuction water is separated by filtration, and the LAF contained in the separated liquid inhaled water is separated and separated. Thus, (LAF) in the buffy coat to separate the interstitial cells from non-proteolytic adipose tissue, as well as to separate LAF from liposuction It is possible to easily remove a large amount of blood cells contained in the blood vessel.

FIG. 1 is a half-sectional perspective view illustrating an apparatus for separating epilepsy cells according to an embodiment of the present invention.
2 is a front cross-sectional view of the stromal cell separator of FIG.
3 is a front sectional view showing a state where the agitator of Fig. 2 is elastically deformed.
FIG. 4 is a schematic view showing a liposuction water stirring process using the stirring means of FIG. 3. FIG.
5 is a front sectional view showing a modified example of the stirring means of Fig.
Fig. 6 is a front sectional view showing a state in which the agitator of Fig. 5 is elastically deformed. Fig.
FIG. 7 is a schematic view showing a process of separating a liquid inhalation from a liposuction material using an epileptiform cell separator according to an embodiment of the present invention, and separating and separating LAF. FIG.
8 is a schematic view showing a process of fractionating LAF from LAF precipitate.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

FIG. 1 is a half-sectional perspective view showing an apparatus for separating epilepsy cells according to an embodiment of the present invention, and FIG. 2 is a front sectional view of the apparatus for separating epilepsy cells of FIG.

1 and 2, an apparatus 1 for separating epilepsy cells according to an embodiment of the present invention includes a container 10, a squeeze net 20, and an agitating means 30. As shown in Fig.

The container 10 has an internal accommodation space for accommodating the obtained liposuction material 100 (see Fig. 7), and a discharge port 13 is formed at a lower portion thereof.

The container 10 according to the present embodiment illustrates that the upper portion 11 is formed in a hollow cylindrical shape and the lower portion 12 is formed in a hollow conical shape in which the sectional area gradually decreases from the upper portion 11 to the lower end portion where the discharge port 13 is formed .

The hollow cylindrical upper portion 11 defines an upper storage space for accommodating therein a squeezed mesh body 20 to be described later and a lower hollow cone shaped bottom portion 12 is formed from the squeezed mesh body 20 And the LAF precipitate 104 precipitated and separated from the liquid inhale material 102 can be separated and discharged through the discharge port 13 more easily.

However, the shape of the container of the present invention is not necessarily limited to the hopper type in which the hollow cylindrical shape and the hollow conical shape are combined as described above. The liquid inhalation material 102 excluding the fat 101 from the liposuction material 100 may be a It is natural that it can be modified and applied so as to have a more various shape as long as it can be separated by filtration using a filter 20.

Meanwhile, a container cover 15 for detachably screwing and sealing the upper open end of the hollow cylindrical top 11 of the container 10 is provided.

One or more injection pipes 16 for injecting the obtained liposuction material 100 are formed in the container lid 15 and an injection pipe opening and closing cap 17 .

The container lid 15 and the injection pipe opening and closing cap 16 are screwed into the upper opening of the container 10 and the upper portion of the injection pipe 16 so as to be openable and closable, It is needless to say that various coupling methods can be applied in addition to a method of being openably and closably coupled to the upper opening of the container 10 and the injection tube 16.

Herein, the diameter of the injection tube 16 is set so that the length of the syringe 60 for injecting the liposuction fluid can be adjusted so that the liposuction 100 sucked through the syringe 60 for injecting the liposuction water can be injected into the container 10. And it is preferably formed so as to correspond to the diameter of the spout portion.

An agitating means 30 to be described later is suspended and fixed on the lower side of the container lid 15 so as to freely move within the drawn net 20 housed in the container 10, 10 to the stand (not shown) or the like.

The discharge tube 40 is connected to the discharge port 13 at the lower portion of the container 10 and only the LAF precipitate 104 precipitated and separated from the liquid suction material 102 is discharged to the discharge tube 40 A discharge tube clamp 50 for controlling the discharge amount discharged from the container 10 is provided.

The diameter of the outlet 13 and the diameter of the discharge tube 40 is also formed with a diameter of a size suitable for separating and discharging the LAF sediment 104 according to the size of the LAF sediment 104, It is preferable that it is formed so as to correspond to the diameter.

The pincushion 20 is accommodated in an internal space on the upper side 11 of the container 10 to filter entangled substances such as lumpy fat tissue and fibrous tissue from the introduced liposuction 100 , And other cells (LAF, blood cells, etc.) other than adipose cells having a predetermined size or less together with the liquid inhale 102 can be collected in the lower portion 12 of the container 10 through the sieve.

The upper end of the trunk body 20 is connected to the upper end 11 of the container 10 so as to be fixed between the container cover 15 and the container cover 15, (25) is formed.

The agitating means 30 is accommodated in the squeezed mesh body 20 so as to agitate the liposuction material 100 inside the squeezed mesh body 20 and to collect agglomerated fat tissue (LAFs, blood cells, etc.) other than the adipocytes contained therein are released to release the entangled substances such as the fibrous tissue, and the liquid inhaled in the filtrate collected in the lower portion 12 of the container 10 103).

Here, the stirring means 30 includes an agitator 31 and an agitator 35 provided in the agitator 31.

The upper part of the agitating body 31 is fixed to the lower part of the container lid 15 so as to be free to move freely, and a plurality of arcuate frames, which are shaped like a head part of the whip, Longitude, and the like).

The agitator 35 is provided on the agitator 31 to shake the container 10 and apply an impact force to the graphene 20 in the course of stirring the liposuction material 100 flowing into the inside of the container 10, Thereby maximizing the effect of providing the inertia force to the agitator body 31 and enhancing the stirring effect of agitating the entangled substances such as fat tissues or fiber tissues in the form of agglomerated bodies inside the shell 20.

FIG. 3 is a front sectional view showing a state where the agitator of FIG. 2 is elastically deformed, and FIG. 4 is a schematic view showing a process of agitating liposuction water using the agitating means of FIG.

3 and 4, in the present embodiment, the transducer 35 is accommodated in the stirring body 31 so that the arcuate frames constituting the stirring body 31 are guided by the introduced liposuction The water 100 is made of a material capable of being elastically deformed such that the water 100 can be directly struck to the inside surface of the screen 20 or the inside surface of the screen 20 after being stirred by the screen 35. FIG.

At this time, it is more preferable that the elastic force of the agitator 31 is formed to be larger than the load of the transducer 35.

That is, the stirrer 31 is normally spaced apart from the inner surface of the squeeze body 20, and the centrifugal force or inertial force generated when the container 10 is shaken to stir the liposuction 100 When the additional momentum is added to the load of the thruster 35, the vertical diameter d1- > d2 of the agitator 31 is elastically deformed so as to be in a state of being adjacent to the inner circumferential surface of the truncated cone 20, The free agitation motion is performed in a state of intermittently hitting the surface.

Thus, the agitator 31 scrapes fat agglomerates blocking the sieve of the sieve net 20 or loosens the entangled substances such as the fibrous tissue while directly hitting the sieve net 20, So that it can be separated and filtered by the lower portion 12 of the container 10 more easily through the sieve of the squeeze net 20.

On the other hand, the agitator 31 also plays a role of restricting the range of motion of the agitator 35. That is, the motion of the transducer 35 is limited to the inside of the stirring body 31 accommodated therein.

FIG. 5 is a front sectional view showing a modification example of the stirring means of FIG. 2, and FIG. 6 is a front sectional view showing a state where the stirring body of FIG. 5 is elastically deformed.

5 and 6, an alternate 135 is hung below the stirrer 131 so that an alternate 135 is provided on the stirrer 131 to provide the necessary inertial force for the stirring movement as described above When the liposuction material 100 is agitated by shaking the container 10, an alternate body 135 is suspended under the agitating body 131 that is elastically deformed, and is deformed so as to be agitated so as to be adjacent to the inside surface of the netting body 20 It is natural that it can be done.

FIG. 7 is a schematic view showing a process of separating a liquid inhalation from a liposuction material using an epileptiform cell separator according to an embodiment of the present invention, and separating and separating LAF. FIG.

Referring to FIG. 7, the above-described liquid-inhalation filtration separation and the LAF precipitation separation process can be collectively performed through the above-described interstitial cell separation device 1.

First, in order to perform the liquid inhalation filtration process, the injection pipe opening / closing cap 17 screwed to the injection pipe 16 formed in the container cover 15 is opened, The liposuction 100 obtained by using the syringe 60 is injected into the container 10.

Then the injection tube 16 is closed again with an injection tube opening and closing cap 17 and then the container 10 is shaken to form entanglement substances such as fat 101 and fibrous structure So that the liquid inhale 103 can be filtered and separated from the lower portion 12 of the container 10 through the sieve of the squeeze body 20.

At this time, in the process of separating the liquid inhale (103) from the liposuction body (100) by means of agitating means composed of the agitating body (31) and the interstitial body (35) (LAF, blood cell, etc.) other than the adipocyte contained therein by grinding entangled substances such as adipose tissue 101 or fibrous tissue in the form of agglomerated lipid tissue 101 in the squeezed body 20 ), The fat masses blocking the sieve of the squeezed mesh 20 are scratched or the squeezed mesh 20 is directly blown to release the entanglement substances such as the fibrous tissue, So that the mesh of the mesh body 20 can pass through more easily.

The stirring body 31 applies an impact force to discontinuously strike the inner surface of the shaped body 20 by the jumping operation induced by the transducer 35 when the container 10 is shaken, So as to increase the effect of squeezing the squeezed fat on the flesh through the squeeze net 20.

At this time, as the container 10 is vigorously shaken, the amount of elastic deformation of the agitator 31 by the agitator 35 increases (d1-> d2) And the force of direct stirring of the agitating member 31 by the agitating member 31 is strengthened, so that the effect of squeezing is increased.

In order to perform the liquid inhalation filtration and separation process, it is possible to buffer the inside of the container 10 so that all of the liposuction 100 is filled. However, by filling the space with the space necessary for the stirring operation, It is more preferable to improve the effect of squeezing the sieve using the agitating member 31 and the stirring effect of the agitating member 31.

In order to perform the LAF precipitation separation process, the liquid inhaled water 103 filtered and separated in the lower portion 12 of the container 10 is left for a predetermined time, and the LAF contained in the liquid inhaled water 103 is introduced into the container 10 The discharge tube clamp 50 is opened to allow the LAF deposit 104 to be separated and discharged through the discharge tube 40 connected to the discharge port 13. [

On the other hand, fat cells contained in liposuction water are smaller than water and floated on the water. Other cells other than adipocytes (LAF, blood cells, etc.) have a specific gravity larger than water, .

Therefore, LAF precipitate 104 contains a large amount of blood cells other than LAF.

A large amount of blood cells contained in the separated LAF precipitate 104 can be fractionated by simple centrifugation using the anticoagulant pretreatment blood 105.

8 is a schematic view showing a process of fractionating LAF from LAF precipitate.

Referring to FIG. 8, the separated LAF precipitate 10 is mixed with the blood 105 pretreated with an anticoagulant, and then the LAF blood mixture 106 is prepared. Then, the LAF blood mixture 106 is centrifuged to collect the LAF in the buffy coat.

Meanwhile, in the course of mixing the blood 105 pretreated with the anticoagulant, the anticoagulant may be further added to the LAF precipitate 104 according to the amount of the LAF precipitate 104, if necessary. In other words, when the amount of the LAF precipitate 104 is large, it is preferable to mix the LAF precipitate 104 with the anticoagulant in advance and then mix it with the blood 105 pretreated with the anticoagulant.

When the LAF blood mixture 106 is centrifuged, the separation of the plasma 107 and the blood cells 108 is performed between the upper layer and the lower layer with the barrier layer 109 interposed therebetween by the specific gravity difference.

On the other hand, LAF is a mononuclear cell whose specific gravity is smaller than that of blood cells (109) and larger than plasma.

Therefore, when the blood 105 pretreated with the anticoagulant is mixed with the LAF precipitate 104 and then centrifuged, a large amount of the hemocytes contained therein are collected under the buffy coat 109, and the LAF is the buffy coat ).

 The upper layer plasma 107 separated by centrifugation is removed by using a pipette, a syringe, etc., and the LAF thus collected is fractionated by sequentially separating the layer 109 (BuffyCoat).

As described above, the epileptiform septum separator 1 of the present invention separates the remaining liquid inhale 103 from the liposuction 100 by filtration and precipitates the LAF contained in the separated liquid inhale 103 The separated blood LAF precipitate 104 is mixed with the blood 105 pretreated with an anticoagulant and centrifuged to collect the LAF in the buffy coat 109 to obtain a fat tissue without protease And it is possible to easily remove a large amount of the LAF contained in the liposome isolated from liposuction.

In the case of the epilepsy cell separating apparatus (1) of the present invention, the epilepsy cell can be easily separated through the above-described fractionation method even in the case of the adipose tissue treated with the proteolytic enzyme in advance.

The present invention can also be used as an injector for injecting a solution containing cells or lumps (blood, SVF, adipose stem cells, bone marrow-derived stem cells, etc.) into a human body.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the spirit and scope of the invention. And it goes without saying that they belong to the scope of the present invention.

1: epileptic cell separator 10: container
11: upper part 12: lower part
13: Outlet 15: Container cover
16: injection tube 17: injection tube opening / closing cap
18: Retaining ring 20: Stretcher
25: netting fixing jaws 30 and 130: stirring means
31, 131: stirrer 35, 135: stirrer
40: exhaust tube 50: exhaust tube clamp
60: Liposuction infusion syringe 70: LAF Sediment separation syringe
100: Liposuction 101: Fat
102: Liquid inhaled substance 103: adipose tissue cells
104: LAF sediment 105: blood coagulant pretreatment blood
106: LAF blood mixture solution 107: plasma
108: Hemocytes 109: Buffy Coat

Claims (12)

A container having an internal accommodation space for storing the obtained liposuction material, and a discharge port formed at a lower portion thereof;
A filter net installed in the upper portion of the container for filtering the liquid inhalation through the lower portion of the liposuction material; And
And an agitating means installed in the internally threaded body so as to agitate the liposuction material in the internally threaded body,

The stirring means
A stirrer suspended in the upper side of the container in a state of being housed inside the mesh net; And
And an agitator provided on the agitator for shaking the container to provide an inertial force when agitating the liposuction material inside the graphene,

Wherein the agitator is made of a material that is elastically deformable.
delete The method of claim 1,
The above-
Wherein the upper part is fixedly suspended on the upper side of the container so as to freely move and the lower part is formed by a plurality of arcuate frames forming an outer shape forming a line of longitude or ellipsoid.
4. The method of claim 3,
And the stirring weight is accommodated in the stirring body.
4. The method of claim 3,
Wherein the stirring weight is suspended from the bottom of the stirring body.
delete The method of claim 1,
Wherein an elastic force of the stirring body is formed larger than a load of the transducer.
8. The method of claim 7,
Wherein when the additional motive force generated when the container is gripped by the container to agitate the liposuction material is added to the load of the collapsible body, And a free agitating motion for intermittently hitting the inner surface of the squeeze netting.
9. The method of claim 8,
The above-
The lipstick is resiliently deformed such that when the lipstick is swirled by the container to stir the liposuction, the jumping operation is induced by the lip,
Wherein an impact force is applied discontinuously to the inner surface of the squeeze net so as to increase the effect of squeezing the squeezed fat through the squeeze net.
The method of claim 1,
And a container cover for covering and sealing the upper opening of the container,

The container cover includes:
An injection tube formed to penetrate to supply the liposuction water; And
And an injection tube opening / closing cap for opening / closing the injection tube.
11. The method of claim 10,
Wherein the stirring body is fixed to the container lid in a freely rotatable manner.
The method of claim 1,
And opening / closing means for opening / closing the outlet of the container,

The outlet opening /
A discharge tube extending from the discharge port; And
And a drain tube clamp for regulating the amount of LAF emissions through the drain tube.

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