KR101884314B1 - Measuring apparatus of bio sensor for simultaneous analysis of hemoglobin and glycated hemoglobin and method thterof - Google Patents

Abstract

The present invention relates to a biosensor measuring device and a method thereof, in which a biosensor measuring device measures a concentration of a target substance, a voltage magnitude of an applied voltage for detecting the concentration of the target substance, A reference electrode, and a plurality of working electrodes, and outputs the applied voltage to the biosensor in accordance with the voltage magnitude and the applied time, A plurality of switches connected to the one counter electrode, the one reference electrode, and the plurality of working electrodes according to the type of the target material; A switching module for opening said plurality of switching modules, And a microcontroller controlling the ON / OFF of the switch and the output of the applied voltage, and outputting the measurement result of the concentration of the target substance according to the reaction current. As described above, according to the present invention, the concentration method of hemoglobin and glycated hemoglobin in a blood sample can be measured quickly and accurately by using the current method instead of the conventional high performance liquid chromatography (HPLC) or the impedance method.

Description

TECHNICAL FIELD The present invention relates to a biosensor measuring device for simultaneous analysis of hemoglobin and glycosylated hemoglobin, and a method of measuring the hemoglobin and glycosylated hemoglobin,

The present invention relates to an apparatus and a method for measuring biosensor for simultaneous analysis of hemoglobin and glycosylated hemoglobin, and more particularly, to a method and apparatus for simultaneous detection of hemoglobin and glycosylated hemoglobin, And more particularly, to a biosensor measurement apparatus and method for analysis.

Diabetes is caused by improper carbohydrate metabolism that does not use the glucose absorbed in the body, and it can cause various complications due to excessive blood sugar in the blood.

There are many ways to diagnose diabetes such as urine glucose measurement and blood glucose measurement. However, urine glucose measurement is not reliable, and blood glucose is inaccurate due to various factors such as eating and exercise. Therefore, in order to manage and treat diabetes, it is effective to measure glycated hemoglobin (HbA1c) in the blood because the average blood glucose level for 3 months is important.

Adult hemoglobin consists of three types: hemoglobin A at 97%, hemoglobin A2 at 2.5%, and hemoglobin F at 0.5%. Double hemoglobin A is a four polypeptide structure consisting of two alpha chains with 141 amino acids and two beta chains with 146 amino acids. When analyzing hemoglobin A by chromatography, it is composed of about 95% of normal hemoglobin and about 5 to 6% of minute glycosylated hemoglobin, and these glycosylated hemoglobin is collectively referred to as hemoglobin A1. Such 80% of hemoglobin A1 is known to have hemoglobin A1a, hemoglobin A1b, hemoglobin A1c, etc. in a form in which glucose is bound to the valine residue at the N-terminal of the beta chain.

The glycosylation process is a very gradual irreversible reaction in which a sugar moiety binds to the amino group of a protein in a non-enzymatic reaction. The glycated hemoglobin is formed by the combination of hemoglobin and blood glucose, and the ratio of hemoglobin to glycated hemoglobin is determined by the degree of exposure of red blood cells and blood glucose. Specifically, in the glycation process, glucose binds to the valine residue of hemoglobin A to form a hemoglobin A1c precursor, which becomes a hemoglobin A1c having a stable ketoamine bond through rearrangement reaction. At this time, the higher the glucose level in the blood, the higher the frequency of contact between glucose and hemoglobin, and the higher the ratio of glycated hemoglobin. Therefore, accurate determination of glucose levels in the blood is possible with the ratio of glycated hemoglobin. In addition, since the lifespan of erythrocytes is about 60 to 120 days, the change in blood glucose concentration can be monitored for a relatively long period of time.

Various methods for measuring glycated hemoglobin in blood have been developed. Currently commercially available methods include ion exchange chromatography, affinity chromatography, electrophoresis, and complex coloring. However, these methods require a great deal of time for a large amount of blood (venous blood), expensive equipment (ultra-high centrifuge, high performance liquid chromatography) for blood pretreatment / analysis and analysis. The measurement of glycated hemoglobin is usually performed by a specialist in the hospital, and the diabetic patients visit the hospital periodically to check the level of glycated hemoglobin and incur the hassle and social costs.

Accordingly, development of a sensor for measuring glycated hemoglobin using a potential difference analysis method is in progress to overcome the above-mentioned problem. However, a method of simultaneously detecting hemoglobin and glycated hemoglobin with high sensitivity using a current method However,

The technology that provides the background of the present invention is disclosed in Japanese Patent Application No. 10-0910982 (issued on Aug. 5, 2009).

SUMMARY OF THE INVENTION The present invention provides a biosensor measuring device and method for simultaneous analysis of hemoglobin and glycosylated hemoglobin for simultaneously analyzing hemoglobin and glycosylated hemoglobin concentration from a blood sample.

According to an aspect of the present invention, there is provided a biosensor measuring apparatus including a sensor for measuring a concentration of a target substance, a voltage magnitude of an applied voltage for detecting the concentration of the target substance, A reference electrode, and a plurality of working electrodes, and outputs the applied voltage to the biosensor in accordance with the voltage magnitude and the applied time, A plurality of switches connected to the one counter electrode, the one reference electrode, and the plurality of working electrodes according to the type of the target material; A switching module that opens said switching module, Controlling an on-off and output of the voltage applied to the switch and includes a micro-controller for outputting a measurement result of the concentration of the said substance in accordance with the reaction current.

The subject material may include glycated hemoglobin and hemoglobin.

The biosensor includes a first sensor for detecting glycated hemoglobin and a second sensor for detecting hemoglobin, wherein the first sensor comprises: a gold nanoparticle layer coated on the surface of the first sensor electrode; a gold nanoparticle layer electrodeposited on the gold nanoparticle layer A conductive polymer layer, and a receptacle of glycated hemoglobin covalently bonded to the conductive polymer layer and an organic electron transport mediator, wherein the second sensor comprises a conductive polymer composite layer electrodeposited on the surface of the second sensor electrode, Wherein the conductive polymer composite layer comprises a conductive polymer and an electrode catalyst material, and the first sensor and the second sensor are disposed so as to face each other, And the like.

Wherein the plurality of working electrodes include a first working electrode for measuring the concentration of glycated hemoglobin and a second working electrode for measuring the concentration of hemoglobin, The second working electrode is connected to the electrode of the second sensor through a short circuit of the switch corresponding to the second working electrode.

Wherein the voltage converter converts the reaction current into a voltage value and outputs a first gain value to select a first resistance value to convert the reaction current detected through the first working electrode into a voltage value according to the first amplification factor set, And a second gain setting unit for selecting a second resistance value to convert the reaction current detected through the second working electrode into a voltage value according to the set second amplification factor.

The input module further receives a current range of the reaction current, and the microcontroller can set a first amplification factor or a second amplification factor corresponding to the current range of the reaction current.

The biosensor may be detachable through a connector connected to the switching module.

A method of measuring a biosensor according to another embodiment of the present invention is a method of measuring a biosensor using a biosensor measuring apparatus, the method comprising: measuring a concentration of a target substance to be measured, Receiving the measurement condition data including the application time of the applied voltage and the current range of the reaction current of the biosensor in accordance with the applied voltage, selecting the working electrode of the biosensor measurement device according to the type of the target substance A step of setting an amplification factor corresponding to a current range of the reaction current, applying an applied voltage to the biosensor through the selected working electrode according to the voltage magnitude and the applied time, And converting the reaction current into a voltage value in accordance with the set amplification factor Step, and a step of outputting the converted voltage value.

As described above, according to the present invention, the concentration method of hemoglobin and glycated hemoglobin in the blood sample can be measured quickly and accurately by using the current method instead of the conventional high performance liquid chromatography (HPLC) or the impedance method. The ratio of the two substances can be measured at the same time without performing the step of separating glycated hemoglobin. Thus, it is possible to effectively diagnose diabetes by the disposable current analysis method of on-the-spot type.

1 is a view for explaining a biosensor measurement system according to an embodiment of the present invention.
2 is a view for explaining a biosensor according to an embodiment of the present invention.
3 is a configuration diagram of a biosensor measuring apparatus according to an embodiment of the present invention.
4 is a circuit diagram of a potentiometer according to an embodiment of the present invention.
5 is a flowchart of a biosensor measurement method according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention.

First, a biosensor measurement system using a biosensor measurement apparatus according to an embodiment of the present invention will be described with reference to FIG. 1 is a view for explaining a biosensor measurement system according to an embodiment of the present invention.

1, a biosensor measurement system according to an embodiment of the present invention includes a user terminal 10, a biosensor 20, and a biosensor measurement apparatus 100. As shown in FIG.

In the biosensor measurement system according to the embodiment of the present invention, the user terminal 10 transmits measurement condition data including the type of the target substance to be measured by the biosensor 20 to the biosensor measurement apparatus 100 .

Then, the biosensor measurement apparatus 100 supplies the applied voltage for detecting the concentration of the target substance to the biosensor 20 coupled according to the measurement condition data, and the biosensor 20 measures the concentration of the reactant And generates a reaction current.

The generated reaction current is input to the biosensor measurement apparatus 100. The biosensor measurement apparatus 100 converts the detected reaction current into a voltage value and transmits data on the voltage value to the user terminal 10. [

Then, the user terminal 10 analyzes the data on the voltage value, analyzes the concentration of the target substance, and provides analysis data to the user.

Next, the configuration of the biosensor measurement system according to the embodiment of the present invention will be described in detail with reference to FIG. 2 to FIG. FIG. 2 is a view for explaining a biosensor according to an embodiment of the present invention, and FIG. 3 is a configuration diagram of a biosensor measuring apparatus according to an embodiment of the present invention.

First, the user terminal 10 includes a computer, a personal digital assistant (PDA), a smart phone, and the like.

The user terminal 10 may be connected to the biosensor measurement device 100 through a wired communication interface such as a USB port or may be connected to the biosensor measurement device 100 through a wireless communication interface such as a LAN, And transmits and receives data to and from the biosensor measurement apparatus 100 according to the embodiment of the present invention.

At this time, the user terminal 10 can transmit and receive data on the measurement condition data or the detected voltage of the reaction current through the wired or wireless communication interface, as well as transmit power for operating the biosensor measurement apparatus 100 .

Meanwhile, the functions such as the input of the measurement condition data of the user terminal 10 and the concentration analysis of the target substance may be combined with the microcontroller of the biosensor measurement apparatus 100, in which case the user terminal 10 may be omitted .

2, the biosensor 20 is a microfluidic dual-type sensor that simultaneously measures the concentrations of hemoglobin and glycosylated hemoglobin. The biosensor 20 is connected to a biosensor measurement device (100).

Specifically, the biosensor 20 includes a first sensor for detecting glycated hemoglobin and a second sensor for detecting hemoglobin. First, the first sensor includes a gold nanoparticle layer coated on the surface of the first sensor electrode, a conductive polymer layer electrodeposited on the gold nanoparticle layer, and a receptor of glycated hemoglobin covalently bonded on the conductive polymer layer and an organic electron transfer mediator .

The second sensor includes a conductive polymer composite layer electrodeposited on the surface of the second sensor electrode and an organic electron transport mediator covalently bonded on the conductive polymer composite layer. The conductive polymer composite layer includes a conductive polymer and an electrode catalyst material .

At this time, the biosensor 20 is formed of a dual-type sensor in which the first sensor and the second sensor are arranged to face each other.

Here, the first sensor electrode and the second sensor electrode can use a screen print carbon electrode, but it is not particularly limited as long as it is an electrode capable of serving as a sensor based on electrochemistry.

The conductive polymer may be selected from the group consisting of p-2,2 ': 5', 5 "-tetiophen-3'-p-benzoic acid (p-TTBA), p-5,2 ' (P-TTCA) and p-2,5-di- (2-thienyl) -1H-pyrrole-P-benzoic acid (p-DTPBA).

And, the receptor of glycated hemoglobin may be any one selected from a boronic acid derivative, an antibody, and an aptamer, but is not limited thereto.

Next, the boronic acid derivative is a receptor of glycated hemoglobin that detects glycated hemoglobin by cis-diol bonding with glycated hemoglobin. Examples of the receptor include glycated hemoglobin, amino-phenylboronic acid (APBA), phenylboronic acid (PBA), thienylboronic acid TBA) and methylboronic acid (MBA), and it is not limited as long as it has a -B (OH) 2 substituent.

Aptamer is a molecule recognition substance that specifically binds to a target molecule. Since it has high affinity and specificity for a target molecule as compared to an antibody, it is composed of a complex with an organic electron transfer mediator, and thus is specific for glycated hemoglobin Can be combined.

Specifically, the aptamer may be the base sequence shown in Sequence Listing 1 (5'-AAA CCT GGT GTC TGG TGG GGG GGG GGC AGG CGG CGA GGA TTG CGG CGC TGC TAC ACA AAC AGA AGG AA-3 '').

Next, the organic electron transfer mediator may be, but is not limited to, methylene blue (MB) or toluidine blue O (TBO).

The organic electron transfer mediator can be used as a receptor for glycated hemoglobin by forming a complex with an antibody and an abtamer, and a sensor produced by covalently bonding to the conductive polymer composite layer by an organic electron transfer mediator alone can be used to measure total hemoglobin Can be detected. Therefore, the concentration of the two substances is measured at the same time without performing the step of separating total hemoglobin and glycosylated hemoglobin based on the dual type sensor in which the first sensor and the second sensor are combined, thereby detecting the ratio of glycated hemoglobin to total hemoglobin can do.

The electrode catalyst material may be any one selected from the group consisting of carbon nanotube (CNT), oxidized graphene (GO), oxidized graphene reduced material (rGO), and mixtures thereof, but is not limited thereto.

Next, a biosensor measuring apparatus 100 according to an embodiment of the present invention will be described in detail.

3, the biosensor measuring apparatus 100 according to the embodiment of the present invention includes an input module 110, a potential varying device 120, a switching module 130, and a microcontroller 140 A converter module 150, and a connector 160, as shown in FIG.

First, the input module 110 receives measurement condition data used for measuring the concentration of the target substance. At this time, the target substance includes glycated hemoglobin and hemoglobin. The measurement condition data may further include the current range of the reaction current, including the kind of the target substance to be measured, the voltage magnitude of the applied voltage for detecting the concentration of the target substance, and the application time of the applied voltage.

Meanwhile, the input module 110 may include a USB / Serial converter 111, an isolator 112, and a regulator 113.

The USB / Serial converter 111 converts a USB (Universal Serial Bus) data communication signal into a USART (Universal Synchronous Asynchronous Receiver / Transmitter) data communication signal. That is, the USB / Serial converter 111 converts the measurement condition data received as the USB data communication signal into the USART data communication signal.

Next, the isolator 112 blocks the high-frequency noise signal that can be input when the communication signal and the power are supplied from the user terminal 10. [ The regulator 113 supplies the power required for the operation of the biosensor measurement apparatus 100 according to the embodiment of the present invention to the microcontroller 140 using the power received from the user terminal 10.

Next, the potential adjuster 120 outputs the applied voltage to the biosensor 20 according to the voltage magnitude and the application time or detects the reaction current of the biosensor 20 according to the applied voltage.

The potential adjuster 120 includes a counter electrode, a reference electrode, and a plurality of working electrodes.

Specifically, the plurality of working electrodes include a first working electrode for measuring the concentration of glycated hemoglobin and a second working electrode for measuring the concentration of hemoglobin. The first working electrode is connected to the electrode of the first sensor through a short circuit of the switch corresponding to the first working electrode and the second working electrode is connected to the electrode of the second sensor through the short- Lt; / RTI >

Then, the electric potential changer 120 converts the reaction current into a voltage value and outputs it. At this time, the potential adjuster 120 includes a second current-voltage conversion amplifier including a first current-voltage conversion amplifier including a first working electrode and a second working electrode.

Here, the first current-to-voltage conversion amplifier includes a first gain setting unit 127 for selecting a first resistance value to convert the reaction current detected through the first working electrode to a voltage value according to the set first amplification factor, And a second gain setting unit 128 for selecting a second resistance value to convert the reaction current detected through the working electrode into a voltage value according to the set second amplification factor. The first gain setting unit 127 and the second gain setting unit 128 can select a resistance value using an analog multiplexer.

Next, the switching module 130 short-circuits or opens at least one of the plurality of switches connected to one counter electrode, one reference electrode, and a plurality of working electrodes, depending on the type of the target material.

For example, the switching module 130 short-circuits the counter electrode, the reference electrode, and the first working electrode and opens the second working electrode when the object material is hemoglobin. On the other hand, when the object material is glycated hemoglobin, the switching module 130 short-circuits the counter electrode, the reference electrode, and the second working electrode and opens the first working electrode.

Meanwhile, the switching module 130 may open all the switches when the measurement device 100 of the biosensor 20 according to the embodiment of the present invention does not detect the concentration of the target substance.

Next, the microcontroller 140 controls the ON / OFF of the plurality of switches and the output of the applied voltage using the measurement condition data. Specifically, the microcontroller 140 controls the switching module 130 to short-circuit or open at least one of the switches connected to the specific electrode according to the type of the target material. In addition, the microcontroller 140 controls the potential adjuster 120 to apply a voltage to the biosensor 20 according to the voltage magnitude and the application time information of the applied voltage.

Then, the microcontroller 140 outputs the measurement result of the concentration of the target substance according to the reaction current. Meanwhile, the microcontroller 140 sets the first amplification factor or the second amplification factor corresponding to the current range of the reaction current.

The converter module 150 converts the digital signal received from the microcontroller 140 into an analog signal and transmits the converted analog signal to the potential varying unit 120. The converter module 150 converts the analog signal received from the potential varying unit 120 into a digital signal And transmits the converted signal to the microcontroller 140, and includes a digital-analog converter 151 and an analog-digital converter 152.

Specifically, the digital-to-analog converter 151 converts the control signal input from the microcontroller 140 into an analog signal and transmits the converted analog signal to the potential changer 120. The microcontroller 140 sends a control signal to the potential adjuster 120 to control the applied voltage output from the potential adjuster 120 to the biosensor 20 or to set the gain of the potential adjuster 120 , And the control signal transmitted at this time is a digital signal. However, since the potential adjuster 120 can be controlled by an analog signal, the digital-to-analog converter 151 converts the digital control signal into an analog control signal and transmits it to the potential adjuster 120.

The analog-to-digital converter 152 converts an output signal obtained by converting the reaction current to a voltage value from the voltage-potential changer 120 into a digital signal, and transmits the digital signal to the microcontroller 140. At this time, the output signal is an analog signal, and the microcontroller 140 processes the digital signal.

According to the embodiment of the present invention, since the biosensor 20 coupled to the biosensor measurement apparatus 100 is a dual sensor, the digital-to-analog converter 151 and the analog-to-digital converter 152 can be dual- have.

Next, the connector 160 connects the biosensor 20 and the biosensor 100. For example, the user can attach and detach the biosensor 20 to the biosensor measuring instrument 100 via the connector 160.

Next, referring to FIG. 4, the circuit configuration of the electric potential varying device according to the embodiment of the present invention will be described. 4 is a circuit diagram of a potentiometer according to an embodiment of the present invention.

The potential adjuster 120 according to the embodiment of the present invention includes four operational amplifiers 121 to 124. [ First, the first operational amplifier 121 is connected to the second stage of the third resistor 125 and the first stage of the fourth resistor 126, the non-inverting terminal is grounded, the output terminal is connected to the counter electrode C, Lt; / RTI > At this time, the first resistor 125 is connected to the signal GND.

The second operational amplifier 122 has an inverting terminal connected to the output terminal, a non-inverting terminal connected to the reference electrode D, and an output terminal connected to the second terminal of the fourth resistor 126.

The third operational amplifier 123 has a non-inverting terminal connected to the microcontroller 140 so that the inverting terminal is connected to the first working electrode E and the first gain setting unit 127, And the output terminal is connected to one end of the analog-to-digital converter and the first gain setting unit 127.

The non-inverting terminal of the fourth operational amplifier 124 is connected to the microcontroller 140 to receive a voltage and the inverting terminal thereof is connected to the second working electrode F and the second gain setting unit 128, And the output terminal is connected to one end of the analog-to-digital converter and the second gain setting unit 128.

On the other hand, the circuit including the third operational amplifier 123 and the first gain setting unit 127 includes a first current-to-voltage conversion amplifier circuit (not shown) for converting a reaction signal detected through the first working electrode E into a voltage signal, And the fourth operational amplifier 124 and the second gain setting unit 128 mean a second current-voltage conversion amplifier circuit for converting a reaction signal detected through the second working electrode F into a voltage signal do.

At this time, the first gain setting unit 127 and the second gain setting unit 128 can select the resistance value through the analog multiplex. Specifically, when the microcontroller 140 sets the first amplification factor and the second amplification factor, the first gain setting section 127 selects the first resistance value corresponding to the first amplification factor set using the analog multiplex, The second gain setting unit 128 selects a second resistance value corresponding to the second amplification factor set using the analog multiplex.

Hereinafter, a biosensor measuring method using the biosensor measuring apparatus according to an embodiment of the present invention will be described with reference to FIG. 5 is a flowchart of a biosensor measurement method according to an embodiment of the present invention.

First, the input module 110 measures the concentration of the target substance, the magnitude of the applied voltage for detecting the concentration of the target substance, the application time of the applied voltage, and the response of the biosensor 20 And the measurement condition data including the current range of the current (S510).

Then, the microcontroller 140 selects the working electrode of the biosensor 20 measuring device 100 according to the type of the target material (S520). For example, the microcontroller 140 can select the working electrode by shorting the switch of the first working electrode for detecting the concentration of hemoglobin when the type of the target material is hemoglobin.

Then, the microcontroller 140 sets the amplification factor corresponding to the current range of the reaction current (S530). The current range of the reaction current means a range of the magnitude of the current generated by the reaction of the biosensor 20 according to the applied voltage. For example, the microcontroller 140 may store the first to fourth current ranges and corresponding amplification ratios, and the microcontroller 140 compares the current range of the input reactive current with the stored first to fourth 4 current ranges are compared, and then the amplification factor of the potential adjuster 120 is set according to the corresponding amplification factor.

Next, the potential adjuster 120 applies the first applied voltage having the first voltage value to the biosensor 20 through the selected working electrode for the first time (S540).

The potential adjuster 120 applies the second applied voltage having the second voltage value to the biosensor 20 through the selected working electrode for a second time (S550).

For example, when the selected working electrode is the first working electrode, the potential adjuster 120 applies the first voltage value of 4V to the biosensor 20 through the first working electrode for the first time (10 seconds) The second voltage value of 3V may be applied to the biosensor 20 through the first working electrode for a second time (20 seconds).

Then, the potential changer 120 detects the reaction current according to the second applied voltage (S560).

The potential changer 120 converts the reaction current into a voltage value according to the set amplification rate (S570).

Then, the microcontroller 140 outputs the converted voltage value (S580). At this time, the microcontroller 140 can remove the noise of the voltage value through the filtering operation, and the noise-removed voltage value is transmitted to the user terminal 10 through the input module 110.

The present invention can quickly and accurately measure the concentrations of hemoglobin and glycosylated hemoglobin in a blood sample by using a current method in place of a measurement method such as a high performance liquid chromatography (HPLC) or an impedance method which has been conventionally used and the measurement of hemoglobin and glycosylated hemoglobin The concentration of both substances can be measured at the same time without going through the separation step, and the ratio can be obtained. Thus, it is possible to effectively diagnose diabetes by the disposable current analysis method of on-the-spot type.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

10: user terminal 20: biosensor
100: Biosensor measuring device 110: Input module
111: USB / Serial converter 112: Isolator
113: Regulator 120: Potentiometer
121: first operational amplifier 122: second operational amplifier
123: third operational amplifier 124: fourth operational amplifier
125: third resistor 126: fourth resistor
127: first gain setting unit 128: second gain setting unit
130: switching module 140: microcontroller
150: converter module 151: digital-to-analog converter
152: Analogue digital converter 160: Connector

Claims (10)

An input module for receiving measurement condition data including a kind of a target substance to be measured, a voltage magnitude of an applied voltage for detecting the concentration of the target substance, and an application time of the applied voltage,
The method of claim 1, further comprising the steps of: outputting the applied voltage to the biosensor in accordance with the voltage magnitude and the applied time; and detecting a reaction current of the biosensor according to the applied voltage Potential shifter,
A switching module for shorting or opening at least one of the plurality of switches connected to the one counter electrode, the one reference electrode, and the plurality of working electrodes according to the type of the target material;
And a microcontroller for controlling the ON / OFF of the plurality of switches and the output of the applied voltage by using the measurement condition data, and outputting a result of measuring the concentration of the target substance according to the reaction current,
Wherein the biosensor includes a first sensor for detecting glycated hemoglobin and a second sensor for detecting hemoglobin,
Wherein the first sensor comprises:
A gold nanoparticle layer coated on the surface of the first sensor electrode, a conductive polymer layer electrodeposited on the gold nanoparticle layer, and a receptor of glycated hemoglobin covalently bonded on the conductive polymer layer and an organic electron transport mediator,
Wherein the second sensor comprises:
A conductive polymer composite layer formed on the surface of the second sensor electrode, the conductive polymer composite layer comprising a conductive polymer and an electrode catalyst material; and an organic electron transport mediator covalently bonded on the conductive polymer composite layer. delete The method according to claim 1,
The biosensor includes:
And a dual type sensor in which the first sensor and the second sensor are disposed to face each other. The method of claim 3,
Wherein the plurality of working electrodes comprise:
A first working electrode for measuring the concentration of glycated hemoglobin and a second working electrode for measuring the concentration of hemoglobin,
Wherein the first working electrode comprises:
The first electrode of the first sensor is connected to the electrode of the first sensor through a short circuit of the switch corresponding to the first working electrode,
Wherein the second working electrode comprises:
And is connected to an electrode of the second sensor through a short circuit of a switch corresponding to the second working electrode. 5. The method of claim 4,
The potential-
Converts the reaction current into a voltage value and outputs it,
A first gain setting unit for selecting a first resistance value to convert the reaction current detected through the first working electrode into a voltage value according to the set first amplification factor,
And a second gain setting unit for selecting a second resistance value to convert the reaction current detected through the second working electrode into a voltage value according to the set second amplification factor. 6. The method of claim 5,
Wherein the input module comprises:
Further receiving a current range of the reaction current,
The microcontroller includes:
And sets the first amplification factor or the second amplification factor corresponding to the current range of the reaction current. The method according to claim 1,
The biosensor includes:
And a connector connected to the switching module. A biosensor measuring method using a biosensor measuring device,
A measurement including a current range of the reaction current of the biosensor in accordance with the type of the target substance to be measured, the voltage magnitude of the applied voltage for detecting the concentration of the target substance, the application time of the applied voltage, Receiving condition data,
Selecting a working electrode of the biosensor measuring device according to the type of the target material,
Setting an amplification factor corresponding to a current range of the reaction current,
Applying an applied voltage to the biosensor through the selected working electrode according to the voltage magnitude and the applied time and detecting a reaction current according to the applied voltage,
Converting the reaction current into a voltage value according to the set amplification rate, and
And outputting the converted voltage value,
Wherein the biosensor includes a first sensor for detecting glycated hemoglobin and a second sensor for detecting hemoglobin,
Wherein the first sensor comprises:
A gold nanoparticle layer coated on the surface of the first sensor electrode, a conductive polymer layer electrodeposited on the gold nanoparticle layer, and a receptor of glycated hemoglobin covalently bonded on the conductive polymer layer and an organic electron transport mediator,
Wherein the second sensor comprises:
A conductive polymer composite layer formed on the surface of the second sensor electrode, the conductive polymer composite layer comprising a conductive polymer and an electrode catalyst material; and an organic electron transport mediator covalently bonded on the conductive polymer composite layer. 9. The method of claim 8,
Wherein the step of detecting the reaction current comprises:
Applying a first applied voltage having a first voltage value to the biosensor via the selected working electrode for a first time,
Applying a second applied voltage having a second voltage value to the biosensor through the selected working electrode for a second time, and
And detecting a reaction current according to the second applied voltage. 9. The method of claim 8,
The biosensor includes:
Wherein the first sensor and the second sensor are arranged to face each other.

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