KR101855806B1 - Artificial blood vessel and manufacturing method thereof - Google Patents

Abstract

The present invention relates to an artificial blood vessel and a method for manufacturing the same. The artificial blood vessel comprises: a first cell layer formed in a tubular shape in a membrane form; a second cell layer formed on an outer side of the first cell layer and surrounding the first cell layer, and formed in a tubular shape in a membrane form; and a polymer layer formed on at least one position of a position inside the first cell layer and a position between the first cell layer and the second cell layer, bonded to at least one of the cell layers, and having a plurality of voids formed thereon. The artificial blood vessel is suitable of a living body due to low toxicity and immune response to a tissue, and has excellent durability since structural stiffness is enhanced to prevent from being twisted, bent or burst.

Description

Technical Field [0001] The present invention relates to an artificial blood vessel,

TECHNICAL FIELD The present invention relates to an artificial blood vessel and a method of manufacturing the same, and more particularly, to an artificial blood vessel suitable for a living body and capable of being well coupled with blood vessels and having improved structural rigidity,

Artificial blood vessels are artificial substitutes of arteries or veins and refer to artificial blood vessels made of synthetic or biopolymers.

In order to form the artificial blood vessel, several considerations must be taken into consideration. In order to be sutured to the blood vessels in the body, the suture should be relaxed.

 Above all, artificial blood vessels must be present in the body for a long time, so they have less toxicity and immune response, and should have structural rigidity and durability to prevent twisting, breaking, and popping.

That is, the artificial blood vessel may be formed of dacron, teflon, polyethylene, polytetraethylene or the like according to the consideration of the artificial blood vessel described above.

This is disclosed in Japanese Laid-Open Patent Publication No. 2014-050412 ("Artificial blood vessel manufacturing method, 2014.03.20.)", A composition for coating a tubular structure containing fibroin and a polyurethane resin and a method for producing an artificial blood vessel using the same .

However, when artificial blood vessels formed with the above-mentioned dexlon, teflon, polyethylene, polytetraethylene, etc. are introduced into the body, external substances are attracted to the body to make them feel a sense of heterogeneity, There is a drawback that it can cause an inflammatory reaction.

In order to solve the above problems, artificial blood vessels can be formed by using smooth muscle cells, fibroblasts and vascular endothelial cells collected from biopsy specimens of patients' skin and veins.

This artificial blood vessel and its preparation method are disclosed in Korean Patent Publication No. 2007-0022352 ("artificial blood vessel and its manufacturing method", 2007.02.26.), Which is excellent in tissue compatibility and suppresses infection or inflammation There is an advantage to be able to do.

However, the artificial blood vessel manufactured in this way has a disadvantage in that it has a low structural rigidity and can be twisted or torn and has low durability.

JP 2014-050412E (2014.03.20) KR 2007-0022352 A (2007.02.26.)

Disclosure of the Invention The present invention has been conceived to solve the above-mentioned problems. It is an object of the present invention to provide an artificial blood vessel which is suitable for a living body with less toxicity and immune response to tissues and has improved structural stiffness to prevent twisting, And a manufacturing method thereof.

To achieve these and other advantages and in accordance with the purpose of the present invention, as embodied and broadly described herein, there is provided an artificial blood vessel comprising: a first cell layer formed in a tubular shape; A second cell layer formed on the outer side of the first cell layer and surrounding the first cell layer, the second cell layer being formed into a tubular shape; And a polymer layer formed on at least one of the inner side of the first cell layer and the first cell layer and the second cell layer and bonded to at least one of the cell layers, the polymer layer having a plurality of voids; And a control unit.

In addition, the first cell layer is a vascular endothelial cell layer, and is formed by spraying a vascular endothelial cell solution, which is a mixed solution of cultured vascular endothelial cells and a gel.

The second cell layer is a smooth muscle cell layer, and is formed by spraying a solution of a smooth muscle cell, which is a solution in which cultured smooth muscle cells and a gel are mixed.

And a third cell layer formed on the outer side of the second cell layer and surrounding the second cell layer, the third cell layer being formed into a tubular shape in the form of a membrane.

In addition, the third cell layer is a fibroblast layer, and is characterized in that a fibroblast solution, which is a mixed solution of the cultured fibroblasts and the gel, is formed by spraying.

And a polymer layer formed between the second cell layer and the third cell layer and surrounding the second cell layer, the polymer layer having a plurality of voids formed therein.

The polymer layer may be a linear structure formed in a straight line or a curved shape along the longitudinal direction and spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Or a combination of two or more of them.

The polymer layer may further comprise: a first layer; And a second layer formed to surround the outer side of the first layer; And a control unit.

The method of manufacturing the artificial blood vessel comprises sequentially collecting solutions from the upper side of the collecting part in the form of tubes or rods rotatable with respect to the central axis through the receiving parts containing the solutions serving as raw materials of the respective cell layers and polymer layers, And the cell layer and the polymer layer are formed through the rotation of the collecting part and the relative movement of the collecting part and the receiving part.

The polymer layer may have a linear structure formed in a straight line or a curved shape along the longitudinal direction of the collecting portion and spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Or a combination of two or more of them.

In addition, the solution contained in each of the receiving portions is adjusted to a predetermined temperature, and the solution is sprayed to the collecting portion to form each cell layer and a polymer layer.

In addition, the receiving portion containing the raw material of the polymer layer is heated and maintained at a temperature higher than the melting temperature of the raw material of the polymer layer, and the receiving portions containing the raw materials of the respective cell layers are maintained at a temperature of -4 to 40 캜 .

INDUSTRIAL APPLICABILITY The artificial blood vessel and the method for producing the same of the present invention are advantageous for a living body because of low toxicity and immune response, and have improved structural rigidity to prevent twisting, breaking, and breaking, thereby providing excellent durability.

1 to 6 are a cross-sectional view and a partial cutaway perspective view of an artificial blood vessel according to a first embodiment of the present invention;
FIGS. 7 and 8 are a cross-sectional view and a partial incision view of artificial blood vessels according to a second embodiment of the present invention;
9 and 10 are a cross-sectional view and a partial incisional view showing artificial blood vessels according to a third embodiment of the present invention.
11 to 14 are a perspective view and a plan view showing a polymer layer 1 layer, a two-layer structure, and a linear structure, a ring structure, a spiral structure, and a mesh structure according to the present invention.
15 is a perspective view showing an embodiment of an artificial blood vessel manufacturing apparatus according to the present invention.

Hereinafter, the artificial blood vessel of the present invention and its manufacturing method as described above will be described in detail with reference to the accompanying drawings.

[Example 1]

1 to 6 are a cross-sectional view and a partially cutaway perspective view of an artificial blood vessel according to a first embodiment of the present invention.

As shown, the artificial blood vessel 1000 of the present invention includes a first cell layer 100 formed in a tubular shape in the form of a membrane; A second cell layer 200 formed on the outer side of the first cell layer 100 so as to surround the first cell layer 100 and formed into a tubular shape in the form of a membrane; And at least one of the inner layer of the first cell layer 100 and the inner layer of the first cell layer 100 and the second cell layer 200 is bonded to one or more of the cell layers 100 and 200, A polymer layer 400 on which the pores 400a are formed; . ≪ / RTI >

First, the first cell layer 100 is formed into a tubular shape in the form of a membrane, and can be formed into a cylindrical tube such as an inner wall of a blood vessel to surround the lumen of the blood vessel. At this time, the first cell layer 100 is preferably formed in a cylindrical shape, but may be formed in various shapes without being limited to a cylindrical shape. That is, the first cell layer 100 may be formed as a tube having both ends open and a peripheral surface closed. The first cell layer 100 may be formed of a material suitable for a living body having less toxicity and immune response to the corresponding blood vessel tissue.

The second cell layer 200 is formed into a tubular shape in the form of a membrane, and can be formed in a cylindrical shape. At this time, the second cell layer 200 is also preferably formed in a cylindrical shape, but may be formed in various shapes without being limited to a cylindrical shape. That is, the second cell layer 200 may be formed as a tube having both ends open and a peripheral surface closed, and may be formed so as to surround the outer side of the first cell layer 100. And the second cell layer 200 may be formed of a material suitable for a living body having less toxicity and immune response to the corresponding blood vessel tissue.

The polymer layer 400 may be formed in a tubular shape having a plurality of voids 400a penetrating the inside and the outside. The polymer layer 400 may be formed on the inner side of the first cell layer 100 and between the first cell layer 100 and the second cell layer 200 to form one or more of the cell layers 100, Lt; / RTI > That is, as shown in FIGS. 1 and 2, a first cell layer 100 is formed in the innermost layer, and a second polymer layer 402 and a second cell layer 200 are sequentially disposed on the outer layer, have. Alternatively, the first polymer layer 401 may be formed on the innermost layer as shown in FIG. 3, and the first cell layer 100 and the second cell layer 200 may be sequentially disposed on the outer side of the first polymer layer 401. The first polymer layer 401 is formed on the innermost layer as shown in FIG. 4 and the first cell layer 100, the second polymer layer 402, and the second cell layer 200 are sequentially arranged on the outer side of the first polymer layer 401, The layers can be combined. At this time, the cavity 400a may be formed in various shapes so that the polymer layer 400 may have elasticity while maintaining structural rigidity.

Thus, the first cell layer 100 and the second cell layer 200 may play a role corresponding to endothelial cells and smooth muscle cells of the blood vessels, respectively, and they may be combined with corresponding vascular tissues to function as artificial blood vessels. At this time, the polymer layer 400 having the gap 400a formed thereon is resilient and structural rigidity can be maintained. Accordingly, the artificial blood vessel of the present invention is suitable for a living body because of its low toxicity and immune response, and its structural rigidity is improved so as not to be twisted, bent or popped, thereby providing excellent durability.

Here, the first cell layer (100) is a vascular endothelial cell layer and may be formed by injecting a vascular endothelial cell solution, which is a mixed solution of cultured vascular endothelial cells and a gel.

That is, the vascular endothelial cells cultured so as to resemble the vascular endothelial cell layer located in the innermost part of the blood vessel are injected with a vascular endothelial cell solution mixed with the gel to form the first cell layer 100 May be formed. At this time, the first cell layer 100 may be formed into a tubular shape in the form of a membrane by spraying or applying the vascular endothelial cell solution to a collecting part in the form of a round bar or a pipe, And the endocytic cell solution is coated on the collecting part to form the first cell layer 100. That is, the first cell layer 100 may be formed into a vascular endothelial cell layer using a vascular endothelial cell solution in which the cultured vascular endothelial cells and the gel are mixed.

The second cell layer 200 is a smooth muscle cell layer, and may be formed by injecting a solution of a smooth muscle cell, which is a mixture of cultured smooth muscle cells and a gel.

That is, the second cell layer 200 is formed by injecting a smooth muscle cell solution mixed with the smooth muscle cells cultured so as to resemble the smooth muscle cell layer existing outside the endothelial cells of the blood vessel, . At this time, the second cell layer 200 may be formed into a tubular shape by spraying or applying a smooth muscle cell solution to the outside of the polymer layer 400 formed on the outer side of the first cell layer 100, and the first cell layer 100 The second cell layer 200 may be formed by immersing the polymer layer 400 on the outer side of the polymer layer 400 and coating it with the solution of the smooth muscle cells on the outer side of the polymer layer 400. [ Alternatively, the first cell layer 100 may be formed into a tubular shape by spraying or applying a smooth muscle cell solution to the outside of the first cell layer 100. In a state where the first cell layer 100 is formed, the first cell layer 100 is immersed in the smooth muscle cell solution, The second cell layer 200 may be formed by coating a smooth muscle cell solution on the outer side of the second cell layer 200.

That is, the second cell layer 200 may be formed as a smooth muscle cell layer by using the smooth muscle cell solution in which the cultured smooth muscle cells and the gel are mixed.

[Example 2]

7 and 8 are a cross-sectional view and a partial incisional perspective view of an artificial blood vessel according to a second embodiment of the present invention.

As shown in the figure, the artificial blood vessel 1000 of the present invention is formed on the outer side of the second cell layer 200 so as to surround the second cell layer 200, and the third cell layer 300 ). ≪ / RTI >

That is, the third cell layer 300 may be formed on the outer side of the second cell layer 200 to surround the second cell layer 200, and the third cell layer 300 may be formed into a film-like tubular shape. At this time, the third cell layer 300 may be formed as a cylindrical tube, and the third cell layer 300 may be formed in a cylindrical shape, but may be formed in various shapes without being limited to a cylindrical shape. That is, the third cell layer 300 is formed as a tube having both ends open and a peripheral surface closed to surround the second cell layer 200, so that the third cell layer 300 is formed on the outer surface of the second cell layer 200, The inner circumferential surface of the base 300 can be coupled. And the third cell layer 300 may be formed of a material suitable for a living body having less toxicity and immune response to the corresponding blood vessel tissue. Further, a polymer layer 400 may be further formed on the outer side of the third cell layer 300.

Here, the third cell layer 300 is a fibroblast layer, and may be formed by injecting a fibroblast solution, which is a mixed solution of the cultured fibroblasts and a gel.

That is, the third cell layer 300 is formed by injecting a fibroblast solution mixed with a fibroblast and a gel cultured so as to be similar to the outer membrane existing outside the smooth muscle cells of the blood vessel, . The third cell layer 300 may be formed into a tubular shape by spraying or applying a fibroblast solution to the outside of the second cell layer 200. The first cell layer 100, the polymer layer 400, 2 cell layer 200 is formed, the third cell layer 300 may be formed by immersing the second cell layer 200 in a fibroblast solution and coating the fibroblast solution on the outer side of the second cell layer 200. That is, the third cell layer 300 can be formed into a fibroblast layer by using a fibroblast solution in which cultured fibroblasts and gel are mixed. In addition, the fibroblast solution may be injected with a collagen material in addition to the fibroblasts and the gel, so that the third cell layer 300 can be formed.

At this time, the solution for forming the cell layers refers to a mixture of cultured cells and gel in each cell layer. The gel may be a variety of materials which are used for spraying the cells to be cultured or for binding between the injected cells. For example, a hydrogel in which most of the solution components are present is recommended, but not limited thereto, Type material can be used.

[Example 3]

9 and 10 are a cross-sectional view and a partial cutaway perspective view of an artificial blood vessel according to a third embodiment of the present invention.

The artificial blood vessel 1000 of the present invention is formed between the second cell layer 200 and the third cell layer 300 so as to surround the second cell layer 200, And a polymer layer 400 on which the first electrode 400a is formed.

This is because the polymer layer 400 is further formed between the second cell layer 200 and the third cell layer 300 and the polymer layer 400 is interposed between the second cell layer 200 and the third cell layer 300 . That is, a polymer layer 400 is further formed on the outer side of the second cell layer 200 so as to surround the second cell layer 200 so that the inside of the polymer layer 400 is bonded to the outside of the second cell layer 200, A third cell layer 300 may be formed on the outer side of the layer 400 to surround the polymer layer 400 so that the inner side of the third cell layer 300 may be bonded to the outer side of the polymer layer 400. The polymer layer 400 formed between the first cell layer 100 and the second cell layer 200 becomes the second polymer layer 402 and the second polymer layer 402 is formed between the second cell layer 200 and the third cell layer 300 The polymer layer 400 to be formed may be the third polymer layer 402. Thus, for example, the first cell layer 100, the second polymer layer 402, the second cell layer 200, the third polymer layer 403, and the third cell layer 300 may be sequentially formed in a radially outward direction have. Further, a polymer layer 400 may be further formed on the outer side of the third cell layer 300. Also, as shown, the first polymer layer 401 and the third polymer layer 403 may be formed without the second polymer layer 402, and the first polymer layer 401 and the second polymer layer 403 may be formed. The layer 402 and the third polymer layer 403 may be both formed. In this case, when the second polymer layer 402 is not present, the second cell layer 200 may be directly bonded to the first cell layer 100. If the third polymer layer 403 is not present, The cell layer 200 may be directly bonded to the third cell layer 300.

[Example of polymer layer]

11 to 14 are a perspective view and a plan view showing a one-layer, two-layer structure and a linear structure, a ring structure, a spiral structure, and a mesh-like structure of the polymer layer according to the present invention.

As shown in the figure, the polymer layer 400 has a linear structure formed in a straight or curved shape along the longitudinal direction and spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Or a combination of two or more of them may be formed.

That is, the polymer layer 400 is formed in a tubular shape having a plurality of air gaps 400a. The polymer layer 400 may have a linear structure, a spiral structure, a ring structure, a mesh structure, or the like so that the air gap 400a may be formed in various shapes And two or more of the above-described forms may be formed in a combined form. The first cell layer 100, the second cell layer 200, and the second cell layer 400, which are bonded together with the polymer layer 400 therebetween, may be easily structured by the polymer layer 400, And the third cell layers 300 can be easily and firmly coupled to each other. The polymer layer 400 may be formed in various shapes other than the above-described structure.

In addition, the polymer layer 400 may have a two-layer structure. That is, as shown in the drawing, a multilayer structure in which a linear structure, a spiral structure, a ring structure, a mesh structure, or the like is stacked in two layers may be formed, and two or more structures may be combined to form a stacked structure of two layers .

Wherein the polymer layer (400) comprises a first layer (410); And a second layer (420) formed to surround the outer side of the first layer (410); . ≪ / RTI >

12 and 13, the polymer layer 400 is formed by combining a linear structure and a ring structure, or a combination of a linear structure and a helical structure, and the polymer layer 400 is formed by combining two or more structures, The bending rigidity acting in the direction perpendicular to the longitudinal direction, and the rigidity against the twist can be improved. At this time, the first layer 410 and the second layer 420 are formed in a structure opposite to the above-described shape, so that a ring structure or a spiral structure is formed in the first layer 410, (420) may be formed. Here, the linear structure may be replaced with a mesh-like structure as shown in Fig. In addition, as described above, the polymer layer may be formed in a two-layer structure, and a variety of modifications such as a fiber bundle or a mesh-like structure in which two or more layers are laminated may be performed.

In particular, the polymer layer 400 in the artificial blood vessel of the present invention can be manufactured by using the artificial blood vessel manufacturing apparatus 1 as shown in FIG. Here, the artificial blood vessel manufacturing apparatus 1 may include a jetting section 10, a heating section 13, a collecting section 20, a jetting position adjusting section 30, and a collecting position adjusting section 40 , The polymer solution may be injected to form the respective layers of the polymer layer 400. More specifically, the polymer solution can be supplied from the receiving portion 11 of the jetting portion 10 and sprayed through the nozzle portion 12. The solution storing portion 11 is heated by the heating portion 13, The polymer stored in the storage part 11 can be melted and the storage part 11 can be maintained at an appropriate temperature. The collecting unit 20 may be provided below the jetting unit 10 to collect the polymer solution injected through the nozzle unit 12 of the jetting unit 10, The polymer layer 400 may be formed on the outer side of the first cell layer 100 by spraying the polymer solution through the nozzle unit 12 in a state where the cell layer 100 is formed. The injection position adjusting unit 30 may move the spraying unit 10 or the collecting unit 20 in the x, y, and z directions to adjust the injection position of the injected polymer solution. The collecting position adjusting unit 40 may include a fixing shaft 41 coupled to both ends of the collecting unit 20 and a driving unit 42 for rotating the fixing shaft 41. The control unit 50 may be connected to the driving unit 42 and the collecting position adjusting unit 40 to control the movement of the jetting unit 10 and the rotation of the collecting unit 20. [ The injection unit 10 can be moved by the injection position adjusting unit 30 and the collecting unit 20 can be rotated by the collecting position adjusting unit 40 so that the polymer layer 400 is formed . The jetting position adjusting unit 30 and the collecting position adjusting unit 40 can be formed such that the jetting unit 10 and the collecting unit 20 can be relatively moved in the x, y, and z directions, 20 can be rotated so that the injection position of the injected polymer solution can be easily adjusted. In addition, the rotation speed of the driving unit 42 is controlled by the control unit 50 so that various types of polymer layers can be formed. Further, the control unit 50 controls the opening and closing of the nozzle unit 12

 In addition, a plurality of jetting portions 10 may be formed to control various jetting portions 10 to form various types of polymer layers. A plurality of jetting portions may contain polymers having different materials, A polymer layer may be formed. In addition, the artificial blood vessel manufacturing apparatus 1 may be provided with a jetting section for forming the first cell layer 100, a second cell layer 200 and a third cell layer 300, or a receiving part containing the respective cell solutions. Also, the opening and closing of the nozzle portions can be controlled through the control unit 50, so that the polymer solution and the respective cell solution can be controlled so as not to be sprayed or injected.

The method for manufacturing the artificial blood vessel includes the steps of injecting a solution through the injecting portions each containing a solution serving as a raw material of each cell layer and the polymer layer on the upper side of the collecting portion 20 in the form of a tube or rod formed to be rotatable about the central axis The cell layer and the polymer layer can be formed through the rotation of the collecting part 20 and the relative movement of the collecting part and the accommodating part, while being sequentially injected into the collecting part.

In other words, although artificial blood vessels can be manufactured by various methods, in the present invention, when the collecting part 20 (see FIG. 1) is moved relative to the collecting part 20 in the form of a tube or rod, Can be rotated to form a cell layer and a polymer layer, and thereby an artificial blood vessel can be manufactured. The collecting unit 20 is connected to the driving unit 42 so as to be rotatable. The collecting unit 20 includes a plurality of jetting units 10 and the first cell layer 100, the second cell layer 200, and the third cell layer 300 And a single injection part in which a material for forming the polymer layer is stored, may be formed as a total of four injection parts. At this time, if the third cell layer 300 is not required, it may be formed as a total of three spraying parts. The case where the artificial blood vessel 1000 is manufactured such that the polymer layer 400 is formed on the outer side of the first cell layer 100 and the second cell layer 200 is formed on the outer side of the first cell layer 100 will be described. While the tubular collecting part 20 is rotated by the centrifugal force, the blood vessel endothelial cell solution is continuously or discontinuously injected into the collecting part 20 in the injection part containing the vascular endothelial cell solution, which is the material of the first cell layer 100, The tab 10 may gradually move to one side of the central axis direction (x-axis direction) of the collecting part 20 to form the tubular first cell layer 100. After the first cell layer 100 is cured, the molten polymer solution, which is the material of the polymer layer 400, is continuously or discontinuously injected into the collecting part 20 from the stored jet part, Various forms of polymer layer 400 with pores 400a may be formed through relative movement of the rotating and collecting part 20 and the jetting part. After the polymer layer 400 is cured, the collecting unit 20 is rotated, and the smooth muscle cell solution is continuously supplied to the collecting unit 20 in a jetting part containing the smooth muscle cell solution, which is the material of the second cell layer 200, The spraying part gradually moves toward one side in the central axis direction of the collecting part 20 so that the tubular second cell layer 200 can be made. At this time, in forming the first cell layer 100 and the second cell layer 200, the collecting unit 20 may be moved in the x-axis direction in a state in which the injecting unit is fixed so as not to move in the x-axis direction. In addition, various artificial blood vessels can be manufactured by changing the moving speed of the jetting part, the rotating direction of the jetting part, the rotating speed of the collecting part, the opening and closing order of the jetting parts, etc. according to the shape of the artificial blood vessel and the arrangement of the material constituting each layer. In this case, the cell layers 100, 200, and 300 may be formed in the same manner as the first cell layer 100, and the polymer layers 401, 402, and 403 may have a length A linear structure formed in a straight line or a curved line along the direction (the central axis direction of the collection portion; the x-axis direction) and spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Or a combination of two or more of them may be formed. In addition, the polymer layer 400 may be formed as a multilayer structure in which a linear structure, a spiral structure, a ring structure, a mesh structure, or the like is stacked in two layers, and two or more structures are combined to form a two- .

In addition, the solution contained in each of the receiving portions 11 may be adjusted to a predetermined temperature, and the solution may be sprayed to the collecting portion 20 to form each cell layer and a polymer layer.

That is, the temperature of the accommodating portion containing the raw material of the polymer layer can be adjusted so that the solid state polymer as the raw material of the polymer layer can be melted. The receiving portion containing the raw material of the polymer layer can be heated up to 300 ° C. Further, the accommodating portions containing the solution serving as the raw material of each cell layer can also be cooled and heated to a preset temperature and the temperature can be adjusted.

At this time, the accommodation portion containing the raw material of the polymer layer can be maintained at a temperature higher than a temperature at which the raw material of the polymer layer can be melted, and the accommodating portions containing the raw materials of the respective cell layers can be maintained at a temperature It can be kept at a low temperature or a normal temperature.

Thus, the polymer can be injected in a state where the polymer is melted at a temperature suitable for forming the polymer layer and maintained at a temperature, so that the formation of the polymer layer can be facilitated. In addition, the receptacles containing the raw material of each cell layer may be cooled or heated to the above-mentioned temperature range to maintain the temperature. Thus, depending on the raw material of the cell layer, the temperature of the body temperature may be maintained to improve the cell characteristics, So that the characteristics of the hydrogel mixed with the cells can be improved.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It goes without saying that various modifications can be made.

1000: Artificial blood vessel
100: first cell layer (vascular endothelial cell layer)
200: second cell layer (smooth muscle cell layer)
300: Third cell layer (fibroblast layer)
400: polymer layer 400a: air gap
401: first polymer layer 402: second polymer layer
403: Third polymer layer
410: first layer 420: second layer
1: Artificial blood vessel manufacturing device
10:
11: receiving part 12: nozzle part
13:
20; Collecting section
30:
40: collecting position adjusting section
41: fixed shaft 42:
50:

Claims (12)

A vascular endothelial cell layer formed by injecting a vascular endothelial cell solution, which is a solution of a cultured vascular endothelial cell and a gel, a first cell layer formed in a tubular shape separated from an adjacent portion;
A smooth muscle cell layer formed by spraying a solution of smooth muscle cells, which is a mixture of cultured smooth muscle cells and a gel, formed on the outer side of the first cell layer to surround the first cell layer, A second cell layer formed of: And
A polymer layer disposed on at least one of the inner side of the first cell layer and the first cell layer and the second cell layer and having a plurality of voids; , ≪ / RTI >
Wherein the first cell layer, the second cell layer, and the polymer layer are bonded to each other.
delete delete The method according to claim 1,
And a third cell layer formed on the outer side of the second cell layer and formed to surround the second cell layer, the third cell layer being formed into a tubular shape in the form of a membrane.
5. The method of claim 4,
The third cell layer is a fibroblast layer,
Wherein the fibroblast solution is a mixture of the cultured fibroblasts and the gel.
5. The method of claim 4,
Further comprising a polymer layer formed between the second cell layer and the third cell layer and surrounding the second cell layer, the polymer layer having a plurality of voids formed therein.
The method according to claim 1,
Wherein the polymer layer
A linear structure formed in a straight line or a curved shape along the longitudinal direction and spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Or a combination of two or more selected from the group consisting of:
8. The method of claim 7,
Wherein the polymer layer
A first layer; And a second layer formed to surround the outer side of the first layer; And an artificial blood vessel.
9. A method for producing an artificial blood vessel according to any one of claims 1 to 8,
While the solution is sequentially injected into the collecting portion through the injecting portions including the receiving portion containing the solution serving as the raw material of each cell layer and the polymer layer on the upper side of the collecting portion shaped like a tube or rod formed to be rotatable with respect to the central axis,
And a cell layer and a polymer layer are formed through rotation of the collecting part and relative movement of the collecting part and the receiving parts.
10. The method of claim 9,
Wherein the polymer layer
A linear structure formed in a straight line or a curved shape along the longitudinal direction of the collecting part and arranged to be spaced apart in the circumferential direction; Spiral structure; A ring-shaped structure formed in a ring shape and spaced apart in the longitudinal direction; And a mesh-like structure formed in a lattice shape; Wherein the first and second electrodes are formed so as to form a combination of any one or two or more selected from the group consisting of the first electrode and the second electrode.
10. The method of claim 9,
Wherein the solutions contained in the respective receptacles are adjusted to a predetermined temperature, and sprayed with a solution to the collecting part to form respective cell layers and polymer layers.
12. The method of claim 11,
The holding portion containing the raw material of the polymer layer is heated and maintained at a temperature higher than a temperature at which the raw material of the polymer layer can be melted and the holding portions containing the raw materials of the respective cell layers are maintained at a temperature of -4 to 40 캜 Wherein the method comprises the steps of:

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