KR101834820B1 - Gene extraction device using magnetic force - Google Patents

Abstract

The present invention relates to a gene extraction device using a magnetic force, and more particularly relates to a gene extraction device using a magnetic force for efficiently extracting gene that is a target of detection in an effluent and promptly discriminating the gene. The present invention comprises: an extraction portion accommodating a detection target bacteria combined with a magnetic particle; and an extraction chamber portion for injecting an extraction solution that extracts gene from the detection target bacteria into the extraction portion, wherein the extraction portion is provided so as to selectively generate the magnetic force to fix the detection target bacteria combined with the magnetic particle in the extraction portion.

Description

[0001] GENE EXTRACTION DEVICE USING MAGNETIC FORCE [0002]

The present invention relates to a gene extracting apparatus using a magnetic force, and more particularly, to a gene extracting apparatus using a magnetic force for efficiently extracting a gene for detection in a desolvation solution and promptly discriminating the gene.

In recent years, studies on nano-biotechnology capable of directly confirming and manipulating biomolecule behavior of nano units such as genes, proteins and cells of an object have been actively conducted. Such nano-biotechnology can be used to diagnose a disease or to determine the type of a target object, and thus the recent industrial field is being expanded.

Particularly, in recent years, the development of a lab-on-a-chip has been actively carried out. A lab-on-a-chip is a type of biochip that allows a small chip to perform research that can be done in a laboratory. Specifically, the lab-on-a-chip is made of plastic, glass, silicon, etc. to create microchannels of nanometer (nm) or less. Through this, labs and researches that can be done in existing laboratories It is a chip made to replace. Because of this practicality, lab-on-a-chip is continuously being developed and used.

However, since the conventional gene discrimination apparatus is large, it is inconvenient to carry and it takes a long time to discriminate the gene, which is a problem in that it is not practical.

In general, in the case of a lab-on-a-chip, the bacteria are desorbed, the bacteria are concentrated, the gene is extracted from the bacteria, and the experiment is performed by injecting the gene extracted in the lab-on-a-chip. At this time, conventionally, there has been a problem that it is necessary to separately perform the steps of concentrating the defective bacteria and extracting the genes, and then injecting the genes individually into the lab-on-a-chip.

In other words, it is difficult to carry a gene discrimination device in the past, and since it takes a long time to prepare for gene discrimination, rapid experimentation is impossible and practicality is low.

Korean Patent Registration No. 10-1421098 (Apr. 14, 2014)

An object of the present invention to solve the above problems is to provide a gene extracting apparatus using magnetic force for efficiently extracting a gene for detection in a desolvating solution and promptly discriminating the gene.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a magnetic resonance imaging apparatus comprising: And an extraction chamber for injecting an extraction solution for extracting a gene in the bacteria to be detected into the extraction unit, wherein the extraction unit selectively generates a magnetic force to cause the detection target bacteria combined with the magnetic particles to be contained in the extraction unit Wherein the magnetic force is provided to be able to be fixed.

In an embodiment of the present invention, the extraction unit may include: an extraction container unit containing the detection target bacteria combined with the magnetic particles; And an extraction magnet unit provided adjacent to the extraction container unit and generating a magnetic force selectively in the extraction container unit.

In the embodiment of the present invention, the extraction unit may further include an extraction motor unit connected to the extraction magnet unit, wherein the extraction motor unit is arranged to adjust a distance between the extraction magnet unit and the extraction container unit .

In the embodiment of the present invention, the extraction magnet unit includes a plurality of magnets provided in the longitudinal direction of the extraction container unit, and adjacent magnets of the plurality of magnets are provided so as to be opposite in polarity have.

In the embodiment of the present invention, the extraction magnet unit may be provided with electromagnets to selectively generate magnetic force in the extraction container unit.

The extraction unit may include an extraction temperature control unit connected to the extraction container unit and heating and cooling the extraction container unit into which the extraction solution is injected at a predetermined temperature. And an extraction cooling unit connected to the extraction temperature control unit to cool the extraction temperature control unit.

In the embodiment of the present invention, the extraction temperature control unit may be characterized in that the extraction container unit is heated and cooled while the magnetic force acting on the extraction container unit is removed.

In the embodiment of the present invention, the extraction temperature control unit may heat the extraction container unit at 90 to 100 degrees for 5 to 20 minutes.

In the embodiment of the present invention, the extraction temperature control unit may cool the heated extraction container unit by 20 to 30 degrees.

The extracting unit may further include an extracting air injecting unit provided upstream of the extracting unit. The extracting air injecting unit injects air into the mixer unit to discharge the extracted gene toward the mixer unit provided downstream of the extracting unit. And air is injected into the extraction portion.

In the embodiment of the present invention, the air injection unit injects air into the extraction unit in a state where a magnetic force is applied to the extraction unit.

According to an aspect of the present invention, there is provided a gene discrimination apparatus to which a gene extracting apparatus using magnetic force is applied.

In order to accomplish the above object, the present invention provides a food poisoning discriminator to which a gene extracting apparatus using magnetic force is applied.

In order to accomplish the above object, the present invention provides a fish species discriminator to which a gene extracting apparatus using magnetic force is applied.

The effect of the present invention according to the above configuration is that the gene can be easily extracted using the magnetic force, and accurate test results can be obtained. Specifically, the present invention can extract the gene from the detection target bacteria by removing the magnetic force when heating the extraction solution in the state of being injected into the extraction container unit. When the extracted gene is transferred to the mixer unit, a magnetic force is applied to fix the detection target bacteria and the magnetic particles, so that only the extracted gene can be conveniently transferred to the mixer unit without a separate process.

In addition, since the process of concentrating the detection target bacteria and extracting the genes is performed automatically, it is possible to quickly and easily identify the genes.

In addition, the gene integration device including the enrichment and gene extraction device using the magnetic force is practical because it is convenient to carry and can identify the gene quickly so that it can be used immediately in the field.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram illustrating the configuration of a device for concentrating and extracting bacteria using a magnetic force according to an embodiment of the present invention. FIG.
FIG. 2 is a diagram illustrating the configuration of a bacterial concentration and gene extraction apparatus using magnetic force according to an embodiment of the present invention.
3 is a flow chart of a method for concentrating bacteria using magnetic force according to an embodiment of the present invention.
FIG. 4 is a flowchart illustrating a step of generating a primary mixed solution of a method for concentrating bacteria using a magnetic force according to an embodiment of the present invention.
FIG. 5 is a flowchart illustrating a step of injecting a generated primary mixed solution of a method for concentrating bacteria using magnetic force into a concentration unit according to an embodiment of the present invention.
6 is a flowchart illustrating a step of concentrating the bacteria to be detected in the method of concentrating bacteria using magnetic force according to an embodiment of the present invention.
FIG. 7 is a flowchart illustrating a step of transferring a second mixed solution of a method for concentrating bacteria using magnetic force to an extracting unit according to an embodiment of the present invention.
FIG. 8 is a flowchart illustrating a step of re-concentrating bacteria to be detected in the method of concentrating bacteria using magnetic force according to an embodiment of the present invention.
9 is a flowchart of a method of extracting a gene using magnetic force according to an embodiment of the present invention.
FIG. 10 is a flowchart illustrating a step of extracting genes of a gene extraction method using magnetic force according to an embodiment of the present invention.
11 is a flowchart illustrating a step of transferring extracted genes of a gene extraction method using magnetic force to a mixer apparatus according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a diagram illustrating a configuration of a bacterial concentration and gene extraction apparatus using magnetic force according to an embodiment of the present invention. FIG. 2 is a diagram illustrating an example of the configuration of a bacterial concentration and gene extraction apparatus using magnetic force according to an embodiment of the present invention. to be.

As shown in FIGS. 1 and 2, a bacterial concentration and gene extraction apparatus 100 using magnetic force includes a mixing unit 110, a buffer unit 120, a concentration pump unit 130, a concentration unit 140, The extraction unit 160, the extraction air injection unit 170, the extraction unit 180, and the mixer transfer valve unit 190 may be included.

The mixing unit 110 is provided upstream of the thickening unit 140 to mix the desorption solution and the magnetic particles and includes a mixing vessel unit 111, a mixing heating unit 112, a mixing motor unit 113, And a mixing valve unit (114).

The mixing vessel unit 111 may be provided so that the desorption solution and the magnetic particles are injected, and the desorption solution and the magnetic particles are supported for a predetermined time. Here, the desolvation solution may be a solution which has been desorbed from food, fish, etc., but is not limited thereto. The magnetic particles may be bonded to the detection object bacteria contained in the desorption solution, and may be particles having magnetic properties.

The mixing heating unit 112 is connected to the mixing container unit 111 and can heat the mixing container unit 111 to a predetermined temperature. At this time, the mixing heating unit 112 may heat the mixing vessel unit 111 to 32 to 42 degrees so that the desorption solution and the magnetic particles can be actively mixed.

The mixing motor unit 113 may be connected to the mixing container unit 111 to apply vibration to the mixing container unit 111. Specifically, the mixing motor unit 113 may mix the desalination solution and the magnetic particles contained in the mixing vessel unit 111 by applying vibration to the mixing vessel unit 111 for 20 to 120 minutes .

Since the mixing vessel unit 111 is heated by the mixing heating unit 112 and the mixing motor unit 113 generates vibration to mix the desorption solution and the magnetic particles, The mixing efficiency of the solution and the magnetic particles can be further improved. And, when the desalination solution and the magnetic particles are mixed, the detection subject bacteria contained in the desorption solution can be combined with the magnetic particles.

The mixing valve unit 114 is provided between the mixing vessel unit 111 and the thickening unit 140 and is capable of selectively providing the mixed primary solution to the thickening unit 140 . Specifically, the mixing valve unit 114 is closed during the mixing of the desorption solution and the magnetic particles in the mixing vessel unit 111, and when the desorption solution and the magnetic particles are mixed, The mixed solution may be supplied to the concentrating part 140.

The buffer unit 120 may be provided upstream of the thickening unit 140 and the buffer unit 120 may be provided to inject the cleaning solution and the transferring solution into the thickening unit 140 . Specifically, the buffer unit 120 may inject the cleaning solution into the thickening unit 140 so that the foreign matter solution contained in the primary mixed solution may be discharged through the foreign matter discharging unit 150. The buffer unit 120 can transfer the secondary mixed solution stored in the concentrating unit 140 to the extracting unit 180 by injecting the transferring solution into the concentrating unit 140. Here, the secondary mixed solution refers to a solution in which the foreign matter solution is discharged and concentrated in the primary mixed solution.

The concentration pump unit 130 may be disposed downstream of the mixing unit 110 and the buffer unit 120 and may be provided upstream of the concentration unit 140. The concentration pump unit 130 may inject the primary mixed solution, the cleaning solution, and the transfer solution into the concentration unit 140.

The concentrating unit 140 concentrates the detection target bacteria by discharging the foreign matter solution from the primary mixed solution in which the desorption solution and the magnetic particles are mixed, selectively generates a magnetic force, And is capable of concentrating the bacteria to be detected. The enrichment unit 140 includes a concentration vessel unit 141, a concentrated magnet unit 142, a concentration motor unit 143, a vibration motor unit 144 and a concentration valve unit 145.

The concentrating container unit 141 may be provided to receive and pass the primary mixed solution. For example, the concentrating container unit 141 may be provided in the form of a tube, and the liquid injected to one side may be discharged to the other side. However, the shape of the concentrating container unit 141 is not limited to the embodiment.

The concentrated magnet unit 142 is provided adjacent to the concentrating container unit 141 and may generate magnetic force selectively in the concentrating container unit 141. For example, the concentrated magnet unit 142 may be provided below the concentrating container unit 141 and may be provided to apply or remove the magnetic force of the concentrating container unit 141.

The condensing magnet unit 142 may be connected to the condensing motor unit 143 to adjust the distance between the condensing magnet unit 142 and the condensing container unit 141. That is, the concentrated magnet unit 142 may move adjacent to the concentrated container unit 141 by the concentrated motor unit 143 to apply a magnetic force to the concentrated container unit 141. Conversely, the condensing magnet unit 142 may be separated from the condensing container unit 141 by the condensing motor unit 143 to remove the magnetic force applied to the condensing container unit 141.

In more detail, the concentrated magnet unit 142 may have a plurality of magnets in the longitudinal direction of the concentrating container unit 141. At this time, the magnets may have different polarities of adjacent magnets. That is, the condensing magnet unit 142 may be provided such that the polarities of the magnets opposed to the concentrating container unit 141 are alternately repeated in the N and S poles.

Also, the concentrated magnet unit 142 may be electromagnetically generated to selectively generate a magnetic force in the concentrating container unit 141. In this way, when the concentrated magnet unit 142 is provided with an electromagnet, a magnetic force may be generated in the concentrated container unit 141 when a current is supplied to the concentrated magnet unit 142. On the contrary, if the current can not flow through the concentrated magnet unit 142, the magnetic force applied to the concentrated container unit 141 can be removed. When the concentrated magnet unit 142 is provided with an electromagnet, the concentrated motor unit 143 may not be provided.

In the case of the concentrated magnet unit 142 provided as an electromagnet as described above, since the magnetic force generated in the concentrating container unit 141 can be controlled by controlling the current flowing in the concentrated magnet unit 142, Easy magnetic control is possible.

The concentrated magnet unit 142 provided as described above can control the flow of the detection subject bacteria contained in the primary mixed solution by controlling the magnetic force applied to the concentrated container unit 141. [

Specifically, when the concentrated magnet unit 142 generates a magnetic force in the concentrating container unit 141, the magnetic particles combined with the detection target bacteria are moved toward the concentrated magnet unit 142 side by the magnetic force, And can be fixed to the inner wall of the container unit 141.

The vibration motor unit 144 is connected to the concentrating container unit 111 and generates vibration in the concentrating container unit 111 when the secondary mixed solution is transferred to the extracting unit 180 And the bacteria to be detected attached to the concentrating container unit (141) are peeled off.

Specifically, the secondary mixed solution can maintain the state of being attached to the concentrated magnet unit 141 inside the concentrated vessel unit 141 even when the concentrated vessel unit 141 is in a state where the magnetic force is removed. Therefore, when the vibration motor unit 144 transfers the secondary mixed solution in the concentrating container unit 141 to the extracting unit 180, Vibration can be applied to the mixed solution to separate from the inner wall. The vibration motor unit 144 provided as described above can increase the transport efficiency of the secondary mixed solution.

The foreign matter discharging unit 150 may be disposed downstream of the thickening unit 140 and the extracting unit 180 and the foreign matter discharging unit 150 may include the thickening unit 140 and the extracting unit 180 ) May be provided to discharge the foreign substance solution.

Specifically, the foreign matter discharge unit 150 includes a foreign matter outlet unit 151, a first discharge valve unit 152, and a second discharge valve unit 152.

The foreign matter outlet unit 151 is disposed on the downstream side of the concentrating unit 140 and the extracting unit 180 so that the foreign matter solution can be discharged.

The first discharge valve unit 152 is provided between the enrichment unit 140 and the discharge port unit 151. When the foreign matter solution having passed through the enrichment unit 140 is transferred to the discharge port unit 151 , And can be opened. The first discharge valve unit 152 may be closed when the second mixed solution is transferred from the enrichment unit 140 to the extraction unit 180.

The second discharge valve unit 152 is provided between the extraction unit 180 and the discharge unit 151 so that when the foreign matter solution having passed through the extraction unit 180 is transferred to the discharge unit 151 , And can be opened. The second discharge valve unit 152 may be closed when the gene extracted by the extractor 180 is transferred to the mixer 200.

The extraction chamber unit 160 can inject the extraction solution for extracting the genes in the detection target bacteria into the extraction unit 180 and the extraction chamber unit 161, the extraction chamber pump unit 162, Unit 163 and an extraction chamber pressure regulating unit 164.

The extraction chamber unit 161 may be provided to receive the extraction solution and may be provided on the upstream side of the extraction unit 180.

The extraction chamber pump unit 162 may be connected to the extraction chamber unit 161 to inject air to supply the extraction solution contained in the extraction chamber unit 161 to the extraction unit 180.

The extraction chamber valve unit 163 is provided between the extraction chamber unit 161 and the extraction unit 180 and may be provided to control whether the extraction solution contained in the extraction chamber unit 161 is supplied or not have.

The extraction chamber pressure regulating unit 164 is connected to the extraction chamber pump unit 162 and the extraction chamber pressure regulating unit 164 is connected to the extraction chamber unit 161 via an air injection tube (Not shown) is connected, the air between the extraction chamber pump unit 162 and the air injection tube is discharged to the outside, and then, the air is sealed. The extraction chamber pressure regulating unit 164 thus provided is configured such that when the air injection tube is connected to the extraction chamber pump unit 162, air between the extraction chamber pump unit 162 and the air injection tube is supplied to the extraction chamber pump It is possible to prevent a problem of flowing into the extraction chamber unit 161 through the unit 162. That is, only a predetermined amount of air can always be injected into the extraction chamber unit 161.

The extraction chamber pressure regulating unit 164 may be opened when the extraction chamber pump unit 162 discharges the extraction solution contained in the extraction chamber unit 161 to regulate the internal pressure. The extraction chamber pressure regulating unit 164 thus provided can improve the safety by allowing the air injection tube to be separated from the extraction chamber pump unit 162 while the internal pressure is adjusted.

The extraction air injection unit 170 is provided on the upstream side of the extraction unit 180 and may include an extraction air injection unit 171 and an extraction air injection valve 172.

The extracting air injection unit 171 extracts air from the extracting unit 180 to discharge the gene extracted by the extracting unit 180 toward the mixer 200 provided downstream of the extracting unit 180 Can be injected. At this time, the extraction air injection unit 171 can inject air into the extraction unit 180 in a state where a magnetic force is applied to the extraction unit 180. In this way, when the extraction unit 180 is applied with a magnetic force, the detection target bacteria and the magnetic particles are attached to the extraction unit 180 and fixed. Therefore, in a state where a magnetic force is applied to the extracting unit 180, only genes extracted from the detection subject bacteria can be transferred to the mixer apparatus 200.

When the extraction air injection unit 171 injects air into the extraction unit 180 and transfers the gene in the extraction unit 180, the extraction chamber unit 160 is also connected to the extraction unit 180 The extraction solution may be further injected to more smoothly transfer the gene.

The extracting unit 180 can re-concentrate the detection target bacteria by discharging the foreign matter solution from the secondary mixed solution in a state in which the detection target bacteria are concentrated by the concentration unit 140, The gene to be detected can be extracted. In particular, the extraction unit 180 may selectively generate a magnetic force to fix the detection target bacteria combined with the magnetic particles in the extraction unit 180, thereby re-concentrating the detection target bacteria and extracting the gene to be detected have.

Specifically, the extraction unit 180 may include an extraction container unit 181, an extraction magnet unit 182, an extraction motor unit 183, an extraction temperature control unit 184, and an extraction cooling unit 185 .

The extraction container unit 181 accommodates the detection target bacteria combined with the magnetic particles, and may be provided so that the secondary mixed solution passes through the extraction container unit 181. For example, the extraction container unit 181 may be provided in a tube shape, but the shape of the extraction container unit 181 is not limited thereto.

The extraction magnet unit 182 is provided adjacent to the extraction container unit 181 and can selectively generate magnetic force in the extraction container unit 181. For example, the extraction magnet unit 182 may be provided below the extraction container unit 181 and may be provided to apply or remove the magnetic force of the extraction container unit 181.

Specifically, the extraction magnet unit 182 may be connected to the extraction motor unit 183 to adjust the distance from the extraction container unit 181. That is, the extraction magnet unit 182 may be moved adjacent to the extraction container unit 181 by the extraction motor unit 183 to apply a magnetic force to the extraction container unit 181. Conversely, the extraction magnet unit 182 may be separated from the extraction container unit 181 by the extraction motor unit 183 to remove the magnetic force applied to the extraction container unit 181.

More specifically, the extraction magnet unit 182 may have a configuration in which a plurality of magnets are provided in the longitudinal direction of the extraction container unit 181. At this time, the magnets may have different polarities of adjacent magnets. That is, the extraction magnet unit 182 may be provided such that the polarities of the magnets opposed to the extraction container unit 181 are alternately repeated in the N and S poles. At the intersection of the N pole and the S pole, a strong magnetic flux is formed. Therefore, since the extraction magnet unit 182 captures and captures magnetic particles intensively at the point where the N pole and the S pole intersect, it is possible to prevent the problem that the detection target bacteria combined with the magnetic particles are lost.

The extraction magnet unit 182 may be electromagnetically generated to selectively generate magnetic force in the extraction container unit 181. When the extraction magnet unit 182 is provided with an electromagnet, a magnetic force may be generated in the extraction container unit 181 when a current is supplied to the extraction magnet unit 182. Conversely, if the current can not flow through the extraction magnet unit 182, the magnetic force applied to the extraction container unit 181 can be removed. If the extraction magnet unit 182 is provided with an electromagnet, the extraction motor unit 183 may not be provided.

In the case of the extraction magnet unit 182 provided as an electromagnet as described above, since the magnetic force generated in the extraction container unit 181 can be controlled by controlling the current flowing in the extraction magnet unit 182, Easy magnetic control is possible.

The extraction magnet unit 182 provided as described above can control the flow of the detection subject bacteria contained in the secondary mixed solution by controlling the magnetic force applied to the extraction container unit 181. [

Specifically, when the extraction magnet unit 182 generates a magnetic force in the extraction container unit 181, the magnetic particles combined with the detection target bacteria are moved toward the extraction magnet unit 182 by the magnetic force, And can be fixed to the inner wall of the container unit 181.

The extraction temperature control unit 184 may be connected to the extraction container unit 181 to heat and cool the extraction container unit 181 into which the extraction solution is injected to a predetermined temperature. The extraction temperature control unit 184 can heat and cool the extraction container unit 181 with the magnetic force acting on the extraction container unit 181 being removed so that the temperature of the secondary mixture solution is uniform .

The extraction container unit 181 can be heated at 90 to 100 degrees for 5 to 20 minutes. If the temperature of the extraction container unit 181 is less than 90 degrees, the extraction solution may not actively active. If the temperature of the extraction container unit 181 exceeds 100 degrees, the gene may be destroyed, The function of the solution may be lost. Thus, the extraction container unit 181 can be heated from 90 to 100 degrees for 5 minutes to 20 minutes.

As described above, the extraction solution contained in the extraction container unit 181 heated in an appropriate range can actively break down the cells of the detection target bacteria to rapidly extract genes.

The extraction temperature control unit 184 can cool the heated extraction container unit 181 to room temperature. At this time, the extraction temperature control unit 184 may cool the temperature of the extraction container unit 181 by 20 to 30 degrees.

The extraction cooling unit 185 may be connected to the extraction temperature control unit 184 to cool the extraction temperature control unit 184. The extraction cooling unit 185 is adapted to be operated simultaneously when the extraction temperature control unit 184 heats the extraction container unit 181 to prevent the failure of the extraction temperature control unit 184 due to overheating And may be operated when the extraction temperature control unit 184 exceeds a predetermined temperature. The extraction cooling unit 184 can operate even when the extraction temperature control unit 184 cools the extraction vessel unit 181 to room temperature so that cooling can be performed more quickly.

The extraction unit 180 may further include a first extraction vessel valve 186 and a second extraction vessel valve 187. The first extraction vessel valve 186 and the second extraction vessel valve 187 may be provided adjacent to both sides of the extraction vessel unit 181, respectively. The first extraction vessel valve 186 and the second extraction vessel valve 187 are connected to the extraction vessel unit 181 when the extraction vessel unit 181 is heated by the extraction temperature control unit 184, It can be controlled to adjust the internal pressure and prevent the contained solution from flowing out.

The mixer transfer valve unit 190 is provided downstream of the extraction unit 180 and may be opened when the extracted gene is transferred to the mixer apparatus 200.

The bacterial concentration and gene extraction device as described above can be applied to a gene integrated chip, a food poisoning discriminator, and a fish species discriminator.

Hereinafter, a method of concentrating bacteria using magnetic force will be described in detail with reference to FIGS. 1, 2 and the following figures.

3 is a flow chart of a method for concentrating bacteria using magnetic force according to an embodiment of the present invention.

3, the method for concentrating bacteria using magnetic force includes a step (S210) of producing a primary mixed solution by mixing a desorption solution and magnetic particles, a step of injecting the generated primary mixed solution into a concentrating part S220), a step S230 of discharging the foreign substance solution from the injected primary mixed solution to concentrate the detection target bacteria (S230), a step S240 of transferring the secondary mixed solution in the concentrated state to the extraction part, And discharging the foreign substance solution from the transferred secondary mixed solution to re-concentrate the detection target bacteria (S250).

FIG. 4 is a flowchart illustrating a step of generating a primary mixed solution of a method for concentrating bacteria using a magnetic force according to an embodiment of the present invention.

4, in step S210 of mixing the desorption solution and the magnetic particles to form a primary mixed solution, a step S211 of injecting the desorption solution and the magnetic particles into the mixing vessel unit is performed .

Next, the step S212 of heating the mixing vessel unit to a predetermined temperature may be performed. At this stage, the mixing vessel unit 111 can be heated to a temperature at which the mixed heating unit 112 can actively mix the desorption solution and the magnetic particles. To this end, the mixing vessel unit 111 may be heated from 32 degrees to 42 degrees.

Next, vibration may be applied to the mixing vessel unit to mix the desorption solution and the magnetic particles to generate a primary mixed solution (S213). In this step, the mixing vessel unit 111 is subjected to vibration for 20 to 120 minutes by the mixing motor unit 113, so that the desorption solution and the magnetic particles can be mixed. In the primary mixed solution thus produced, the detection target microorganism and the magnetic particles may be combined.

FIG. 5 is a flowchart illustrating a step of injecting a generated primary mixed solution of a method for concentrating bacteria using magnetic force into a concentration unit according to an embodiment of the present invention.

Referring to FIG. 5, the step of injecting the generated primary mixed solution into the concentrating unit (S220) may be performed by first performing a step S221 of generating a magnetic force in the concentrating container unit using the concentrated magnet unit have.

Concretely, in the step S221 of generating a magnetic force to the concentrating container unit by using the concentrated magnet unit, the concentrated magnet unit 142 is disposed adjacent to the concentrating container unit 141 by the concentrating motor unit 143 So that a magnetic force can be generated in the concentrating container unit 141.

Alternatively, when the concentrated magnet unit 142 is provided with an electromagnet, in the step S221 of generating a magnetic force in the concentrating container unit using the concentrated magnet unit, a current is supplied to the concentrated magnet unit 142, It is also possible to generate a magnetic force in the magnet 141.

Next, the step of injecting the primary mixed solution into the concentrating container unit using the concentration pump unit (S222) may be performed. At this stage, the detection subject bacteria contained in the primary mixed solution may be attached to the inner wall of the concentrating container unit 141 by magnetic force in a state of being combined with the magnetic particles.

6 is a flowchart illustrating a step of concentrating the bacteria to be detected in the method of concentrating bacteria using magnetic force according to an embodiment of the present invention.

Referring to FIG. 6, the step S230 of discharging the foreign matter solution from the injected primary mixed solution to concentrate the detection target bacteria is performed first (step 231) of injecting the cleaning solution into the concentration part . Prior to this step, the first discharge valve unit 152 is opened and the buffer unit 120 can inject the cleaning solution into the concentrating container unit 141.

Next, the foreign substance solution may be discharged to the foreign substance discharging unit together with the washing solution (S232) to concentrate the detection target bacteria. At this stage, the cleaning solution can transfer the foreign matter solution in the primary mixed solution contained in the concentrating container unit 141 to the foreign matter outlet unit 151 and discharge it. At this time, the bacteria and the magnetic particles to be detected attached to the inner wall of the concentrating container unit 141 are not conveyed to the foreign matter outlet unit 151 together with the cleaning solution, but by the magnetic force of the concentrated magnet unit 142 By keeping the fixed state, it can be concentrated.

FIG. 7 is a flowchart illustrating a step of transferring a second mixed solution of a method for concentrating bacteria using magnetic force to an extracting unit according to an embodiment of the present invention.

7, in step S240, the magnetic force generated in the concentrating container unit is removed, and the extraction magnet unit is used to extract the secondary mixed solution in the extracting container (S241) of generating a magnetic force to the unit. At this stage, the extraction magnet unit 182 may move adjacent to the extraction container unit 181 by the extraction motor unit 183 to generate a magnetic force in the extraction container unit 181.

Alternatively, in the step (S241) of removing magnetic force generated in the concentrating container unit and generating magnetic force in the extraction container unit using the extraction magnet unit when the extraction magnet unit 182 is provided as an electromagnet, 182 to generate a magnetic force in the extraction container unit 181. [

Conversely, the concentrating unit 140 separates the concentrated magnet unit 142 from the concentrating container unit 141, or prevents current from flowing through the concentrated magnet unit 142, Can be removed.

Next, the step of generating vibration (S242) in the concentrating container unit may be performed. At this stage, the vibration motor unit 144 provided in the enrichment unit 140 generates vibration in the enrichment container unit 141 to remove the detection target bacteria attached to the enrichment container unit 141 . That is, the detection target bacteria adhering to the inner wall of the thickening part 140 can be more quickly transferred to the extracting part 180.

Next, the transferring solution may be injected into the concentrating unit to transfer the secondary mixed solution to the extraction container unit (S243).

Specifically, the buffer unit 120 injects the transfer solution into the concentrating unit 140 while the enrichment valve unit 145 is opened, thereby to transfer the secondary mixed solution to the extraction container unit 181 .

FIG. 8 is a flowchart illustrating a step of re-concentrating bacteria to be detected in the method of concentrating bacteria using magnetic force according to an embodiment of the present invention.

Referring to FIG. 8, in step S250 of discharging the foreign substance solution from the transferred secondary mixed solution to re-concentrate the detection target bacteria, the detection target bacteria combined with the magnetic particles are collected in the extraction container Unit (step S251).

Specifically, in this step, the magnetic force generated in the concentrating container unit is removed, and the magnetic force applied to the extraction container unit 181 in the step (S241) of generating magnetic force to the extraction container unit using the extraction magnet unit The bacteria to be detected contained in the secondary mixed solution passing through the container unit 181 can be attached to the extraction container unit 181. [

Next, the foreign substance solution transferred together with the transporting solution may be discharged to the foreign matter discharging unit, and the step of re-concentrating the detection target bacteria (S252) may be performed. Prior to this step, the second discharge valve unit 153 is switched to the open state. At this stage, the foreign matter solution other than the detection target bacteria combined with the magnetic particles may be transferred to the foreign matter discharging unit 150 together with the transferring solution and discharged. When the foreign matter solution is completely discharged through the discharge unit 150, only the detection target bacteria adhering to the inner wall of the extraction container unit 181 are left by the magnetic force, so that the re-concentration can be performed.

The method of concentrating bacteria using the magnetic force provided as described above can be applied to gene integrated chips, food poisoning discriminators, fish species discriminators, and the like.

Hereinafter, a method of extracting genes using magnetic force will be described in detail with reference to FIGS. 1, 2, and the following drawings.

9 is a flowchart of a method of extracting a gene using magnetic force according to an embodiment of the present invention.

9, the method for extracting a gene using a magnetic force includes a step S310 of injecting an extraction solution into an extraction part containing a detection target bacteria combined with magnetic particles (S310), heating the extraction part to a predetermined temperature, (S320) a step of extracting the gene from the bacteria, and a step (S330) of cooling the extracting unit to room temperature, and transferring the extracted gene toward the mixer apparatus (S340).

More specifically, in the step S310 of injecting the extraction solution into the extraction unit containing the detection target bacteria combined with the magnetic particles, the extraction chamber unit 160 includes the extraction unit 180 ). ≪ / RTI > Here, the extraction solution may be a solution capable of extracting the gene in the bacteria to be detected by disrupting the bacteria to be detected.

FIG. 10 is a flowchart illustrating a step of extracting genes of a gene extraction method using magnetic force according to an embodiment of the present invention.

10, the step of extracting a gene from the detection target bacteria by heating the extracting unit to a predetermined temperature (S320) includes first removing the magnetic force applied to the extracting unit by using the extracting magnet unit (S321).

More specifically, in the step S321 of removing the magnetic force applied to the extraction unit using the extraction magnet unit, the extraction magnet unit 182 is separated from the extraction container unit 181 and is connected to the extraction unit 180 The applied magnetic force can be removed.

Alternatively, in a case where the extracted magnet unit 182 is provided with an electromagnet, in the step S321 of removing the magnetic force applied to the extraction unit using the extracted magnet unit, the current flowing in the extracted magnet unit 182 is controlled So that the magnetic force applied to the extraction unit can be removed.

Next, the extracting unit may be heated to a predetermined temperature to extract a gene from the detection target organism (S322). At this stage, the extraction section 180 can be heated at 90 to 100 degrees for 5 to 20 minutes. If the temperature of the extraction container unit 181 is less than 90 degrees, the extraction solution may not actively active. If the temperature of the extraction container unit 181 exceeds 100 degrees, the gene may be destroyed, The function of the solution may be lost. Thus, the extraction container unit 181 can be heated from 90 to 100 degrees for 5 minutes to 20 minutes.

As described above, the extraction solution contained in the extraction container unit 181 heated in an appropriate range can actively break down the cells of the detection target bacteria to rapidly extract genes.

After the extracting unit is heated to a predetermined temperature and the gene is extracted from the detection target bacteria (S320), the extraction unit may be cooled to room temperature (S330). At this stage, the extraction container unit 181 can be quickly cooled by 20 to 30 degrees by the extraction temperature control unit 184 and the extraction cooling unit 185.

11 is a flowchart illustrating a step of transferring extracted genes of a gene extraction method using magnetic force to a mixer apparatus according to an embodiment of the present invention.

Referring to FIG. 11, in step S340 of transferring the extracted gene toward the mixer device, a step S341 of applying a magnetic force to the extraction unit may be performed. At this stage, the detection target bacteria contained in the extraction container unit 181 are in a state of being fixed to the inner wall of the extraction container unit 181 by the magnetic force because they are in a state of being coupled with the magnetic particles. However, the gene extracted from the detection target bacteria is not fixed to the inner wall of the extraction container unit 181 because it is not coupled with the magnetic particles.

Next, the step of transferring the gene extracted by the extracting unit to the mixer apparatus (S342) may be performed. At this stage, the extraction air injection unit 170 may open the extraction air injection valve 172 to inject air into the extraction unit 180. At this time, the second discharge valve unit 153 is closed, and the mixer transfer valve unit 190 may be in an open state. In this state, the air injected into the extraction unit 180 may transfer the gene to the mixer apparatus 200. At this time, the bacteria to be detected and the magnetic particles attached to the extraction container unit 181 are not transferred to the mixer device 200, so they can be separated from the gene and discharged naturally.

In the step S342 of transferring the gene extracted by the extracting unit toward the mixer apparatus, the extraction chamber 160 further injects the extraction solution into the extraction unit 180 to smoothly transfer the gene .

The magnetic extraction method using the magnetic force as described above can be applied to a gene integrated chip, a food poisoning discriminator, a fish species discriminator, and the like.

The method of concentrating and extracting a gene using a magnetic force and the method of concentrating and extracting a gene using the magnetic force described above can quickly and easily discriminate a gene because the processes of concentrating bacteria and extracting genes are all performed automatically.

In addition, the gene integrating apparatus including the enriching and gene extracting apparatus using the magnetic force of the present invention is practical because it is convenient to carry and can identify the gene quickly so that it can be used immediately in the field.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

100: Bacterial concentration and gene extraction device using magnetic force
110: mixing part 111: mixing container unit
112: mixing heating unit 113: mixing motor unit
114: mixing valve unit 120: buffer unit
121: buffer container unit 122: buffer valve unit
130: enrichment pump unit 140: enrichment unit
141: Enrichment container unit 142: Enrichment magnet unit
143: Enrichment motor unit 144: Vibration motor unit
145: Concentration valve unit 150: Foreign matter discharging unit
151: Foreign matter outlet unit 152: First discharge valve unit
153: second discharge valve unit 160: extraction chamber part
161: Extraction chamber unit 162: Extraction chamber pump unit
163: Extraction chamber valve unit 164: Extraction chamber pressure regulation unit
170: extraction air injection unit 171: extraction air injection unit
172: Extraction air injection valve 180: Extraction section
181: Extraction container unit 182: Extraction magnet unit
183: Extraction motor unit 184: Extraction temperature control unit
185: extraction cooling unit 186: first extraction vessel valve
187: Second extraction container valve 190: Mixer feed valve part
200: Mixer device

Claims (14)

An extraction unit for containing the detection target bacteria combined with the magnetic particles;
An extraction chamber for injecting an extraction solution for extracting genes in the bacteria to be detected into the extraction unit; And
And an extraction air injection unit provided on the upstream side of the extraction unit,
Wherein the extraction unit is provided to selectively generate a magnetic force to fix the detection target bacteria combined with the magnetic particles in the extraction unit,
The extraction air injection unit,
Wherein the extracting unit injects air into the extracting unit so as to discharge the extracted gene toward a mixer unit provided downstream of the extracting unit. The method according to claim 1,
The extracting unit extracts,
An extraction container unit in which the detection target bacteria combined with the magnetic particles are accommodated; And
And an extracting magnet unit provided adjacent to the extraction container unit and generating a magnetic force selectively in the extraction container unit. 3. The method of claim 2,
The extracting unit extracts,
And an extraction motor unit connected to the extraction magnet unit,
Wherein the extraction motor unit is arranged to adjust a distance between the extraction magnet unit and the extraction container unit. The method of claim 3,
Wherein the extraction magnet unit comprises:
And a plurality of magnets provided in the longitudinal direction of the extraction container unit,
And the adjacent magnets of the plurality of magnets are provided so that their polarities are opposite to each other. 3. The method of claim 2,
Wherein the extraction magnet unit comprises:
Wherein the electromagnet generates a magnetic force selectively in the extraction container unit. 3. The method of claim 2,
The extracting unit extracts,
An extraction temperature control unit connected to the extraction container unit and heating and cooling the extraction container unit into which the extraction solution is injected at a predetermined temperature; And
Further comprising an extraction cooling unit connected to the extraction temperature control unit to cool the extraction temperature control unit. The method according to claim 6,
Wherein the extraction temperature control unit comprises:
And the extraction container unit is heated and cooled in a state in which the magnetic force acting on the extraction container unit is removed. 8. The method of claim 7,
Wherein the extraction temperature control unit comprises:
And the extraction container unit is heated at 90 to 100 degrees for 5 to 20 minutes. 9. The method of claim 8,
Wherein the extraction temperature control unit comprises:
And cooling the heated extraction container unit by 20 to 30 degrees. delete The method according to claim 1,
The extraction air injection unit,
And injecting air into the extraction unit in a state where a magnetic force is applied to the extraction unit. A gene discrimination apparatus to which the gene extracting apparatus using the magnetic force according to claim 1 is applied. Food poisoning discriminator using the magnetic extraction device according to claim 1. A fish species discriminator applying the magnetic extraction apparatus according to claim 1.

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