KR101816610B1

KR101816610B1 - SNP marker for prediction of dog's body height and prediction method using the same

Info

Publication number: KR101816610B1
Application number: KR1020150159299A
Authority: KR
Inventors: 최봉환; 박종은; 임다정; 임영조
Original assignee: 대한민국
Priority date: 2015-11-13
Filing date: 2015-11-13
Publication date: 2018-02-22
Also published as: KR20170056724A

Abstract

More particularly, the present invention relates to a SNP marker composition capable of predicting height of dog dogs, and a method of accurately and rapidly determining dog height using the SNP marker A microarray, and a dog height prediction method. By utilizing the SNP marker of the present invention, it is possible to predict the dog height quickly and accurately.

Description

SNP markers for predicting elevation height of dogs and prediction methods using the same

The present invention relates to a SNP marker for predicting height of a dog and a method for predicting height of a dog using the SNP marker.

The height of the dog means the length from the top of the shoulder to the point where the paw touches the ground, and the length means the length from the end of the sternum (anterior bone) to the tip of the sciatica.

The height of the dog is one of the representative economic traits along with the length of the dog. As dog owners have different preferences for the outer shape, the need for early prediction through genetic testing is emerging.

As a precedent literature on genetic markers for the traits of dogs, "Final Report on the Development of Small Slaughtering by Molecular Sarcoma" Research Institution: Kyungpook National University, organized by the Ministry of Agriculture and Forestry in 2004).

The use of the gene-related gene markers of the dogs can satisfy the preference of the dog lovers for the height of the body height, and can restrict the indiscriminate breeding for producing the high or low body height, It is expected that it will be able to establish cultural settlement and to acquire scientific value and basic medical information about growth of animals by discovering genes related to dog height.

Under these technical backgrounds, the present inventors have made intensive efforts to develop gene markers for early diagnosis by identifying genes related to the height of dogs.

Accordingly, it is an object of the present invention to provide a SNP marker composition for predicting dog height.

In addition, the present invention provides a method for predicting the height of a dog using the SNP marker at an early stage.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a polynucleotide comprising: (a) a polynucleotide having a 61st base of A or G in the polynucleotide of SEQ ID NO: 1, an inner nucleotide sequence of SEQ ID NO: 1, A polynucleotide consisting of 121 consecutive bases or a complementary polynucleotide thereof; (b) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 2 and the 61rd base is the internal base sequence of SEQ ID NO: 2; or Complementary polynucleotides thereof; (c) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 3 and the 61rd base is the internal base sequence of SEQ ID NO: 3, or Complementary polynucleotides thereof; (d) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 4 and the 61rd base is the base sequence of SEQ ID NO: 4; or Complementary polynucleotides thereof; (e) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 5, and the 61rd base is the internal base sequence of SEQ ID NO: 5, or Complementary polynucleotides thereof; (f) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 6, and the 61rd base is the internal base sequence of SEQ ID NO: 6; or Complementary polynucleotides thereof; (g) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or C in the polynucleotide shown in SEQ ID NO: 7, and the 61rd base is the base sequence of SEQ ID NO: 7; Complementary polynucleotides thereof; (h) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide of SEQ ID NO: 8 and the 61rd base is the base sequence of SEQ ID NO: 8, or Complementary polynucleotides thereof; (i) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 9 and the 61rd base is the internal base sequence of SEQ ID NO: 9, or Complementary polynucleotides thereof; (j) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 10 and the 61rd base is the internal base sequence of SEQ ID NO: 10; or Complementary polynucleotides thereof; (k) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 11 and the 61rd base is the internal sequence of SEQ ID NO: 11; or Complementary polynucleotides thereof; And (l) a polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 12, and the 61rd base is the base sequence of SEQ ID NO: 12 Or a complementary polynucleotide thereof. The SNP marker composition according to any one of claims 1 to 5,

According to another aspect of the present invention, there is provided a kit for estimating height of height of a dog including the composition.

According to another aspect of the present invention, there is provided a microarray for predicting dog height including the composition.

According to yet another aspect of the present invention, there is provided a method for detecting a nucleic acid molecule comprising: a) separating a nucleic acid molecule from a dog; And b) identifying the base type of the SNP corresponding to the 61st base of the polynucleotide of any one of SEQ ID NOS: 1 to 12 in the separated nucleic acid molecule.

According to the embodiment of the present invention, the height of the dogs can be predicted early, and the effect of improving the height of the dogs and the preference of the dogs can be achieved.

In addition, it is possible to limit the indiscriminate breeding to produce high and low individuals, and thus it can contribute to the protection welfare policies of the dogs, and it is possible to settle the dogs culture.

Furthermore, it is expected that it will be possible to obtain academic value and basic medical information about the growth of animals by discovering genes related to height of dogs.

Hereinafter, the present invention will be described in more detail.

According to one embodiment of the present invention, the composition comprises an agent capable of detecting or amplifying the SNP marker.

In the present invention, the kit may be, but is not limited to, an RT-PCR kit or a microarray chip kit including a preparation capable of detecting or amplifying SNP markers.

The RT-PCR kit can comprise a respective pair of primers specific for the marker gene and can be used in combination with other test tubes or other appropriate containers, reagents necessary for PCR amplification, such as buffers, DNA polymerases (e.g., Thermus aquaticus Taq), Thermus thermophilus (Tth), Thermus filiformis, Thermisflavus, Thermococcus literalis or Pyrococcus furiosus (Pfu)), DNA polymerase joins and dNTPs.

The kit may be made from a number of separate packaging or compartments containing the reagent components described above.

In the present invention, a microarray means a group of polynucleotides immobilized on a substrate at a high density, and the polynucleotide group means a microarray immobilized in a constant region. Such microarrays are well known in the art. The microarrays are described, for example, in U.S. Patent Nos. 5,445,934 and 5,744,305, the contents of which are incorporated herein by reference.

According to an embodiment of the present invention, the separated nucleic acid molecule is amplified in step b).

According to an embodiment of the present invention, the amplified gene product may be purified to analyze the base sequence or hybridize with the SNP marker of the present invention.

Methods for amplifying the nucleic acid molecules include PCR, ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification system, , Strand displacement amplification or amplification through a Q [beta] replicase, or any other suitable method for amplifying nucleic acid molecules known in the art. Among them, PCR is a method of amplifying a target nucleic acid from a pair of primers that specifically bind to a target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used. The PCR can be carried out using a PCR reaction mixture containing various components known in the art necessary for the PCR reaction.

In the present invention, hybridization refers to a process in which two complementary strands of a nucleic acid are combined to form a double stranded molecule (hybrid). In the method of the present invention, the hybridization is carried out under high stringency hybridization conditions.

To detect the degree of hybridization, the target sequence may be labeled with a detectable labeling substance. In one embodiment, the labeling material may be a fluorescent, phosphorescent or radioactive substance, but is not limited thereto. Preferably, the labeling substance is Cy-5 or Cy-3. When the target sequence is amplified, PCR is carried out by labeling the 5'-end of the primer with Cy-5 or Cy-3, and the target sequence may be labeled with a detectable fluorescent labeling substance. In addition, if the radioactive isotope such as P32 or S35 is added to the PCR reaction solution during the PCR, the amplification product may be synthesized and the radioactive substance may be incorporated into the amplification product and the amplification product may be labeled as radioactive.

According to one embodiment of the present invention, when the base type of the SNP is identified from the isolated nucleic acid molecule, when the 61st base is A in the polynucleotide of SEQ ID NO: 1; When the 61st base in the polynucleotide represented by SEQ ID NO: 2 is A; When the 61st base is G in the polynucleotide represented by SEQ ID NO: 3; When the 61st base is G in the polynucleotide represented by SEQ ID NO: 4; When the 61st base in the polynucleotide shown in SEQ ID NO: 5 is A; When the 61st base in the polynucleotide of SEQ ID NO: 6 is A; When the 61st base in the polynucleotide represented by SEQ ID NO: 7 is C; When the 61st base in the polynucleotide of SEQ ID NO: 8 is A; The polynucleotide of SEQ ID NO: 9 has the 61st base of G; When the 61st base in the polynucleotide of SEQ ID NO: 10 is A; When the 61st base is G in the polynucleotide shown by SEQ ID NO: 11; And when the 61st base is G in the polynucleotide represented by SEQ ID NO: 12, it is possible to provide a method of determining that the body weight is relatively high when at least one selected from the group consisting of:

Hereinafter, the present invention will be described in more detail with reference to Examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.

Example Canine 170K SNP chip analysis for 1. dog

DNA was extracted from the blood of 50 dogs using the Wizard Genomic DNA Purification Kit (Promega, Madison, Wis., USA) and SNP genotyping was performed using CanineHDBeadChip (Illumina, San Diego, CA, USA).

&Lt; Amplification >

○ Materials and equipment

1. Reagents

2. Organization

Centrifuge, Vortex, Illumina Hybridization oven

○ Experimental Method

1. Place 20 μl of MA1 on a MIDI plate (labeled MSA3 plate) with an MSA3 bar code.

2. Place 4 ul of DNA into MSA3 plate.

3. Record the DNA ID and the location of the MSA3 plate on the lab tracking form.

4. Add 4 ul of 0.1 N NaOH to each well of MSA3 plate containing MA1 and DNA.

5. Cover the MSA3 plate using a 96-well cap mat and vortex for 1 minute at 1600 rpm.

6. Centrifuge at 280 × g for 1 minute.

7. React at room temperature for 10 minutes.

8. Add 34ul MA2 to each well of the MSA3 plate containing the sample.

9. Add 38ul MSM to each well of the MSA3 plate containing the sample.

10. Cover the cap mat and centrifuge at 280 × g for 1 minute.

11. React for 20-24 hours in an Illumina Hybridization oven at 37 ° C.

(Amplification)

<Day 2 of experiment - Fragment>

○ Materials and equipment

○ Experimental Method

1. Remove the plate from the oven and centrifuge at 50 × g for 1 minute.

2. Place 25 ul of FMS into each well containing sample.

3. Cover the MSA3 plate with a cap mat and vortex for 1 minute at 1600 rpm.

4. Remove the plate and centrifuge at 50 × g for 1 min.

5. Allow to react for 1 hour at 37 ℃ heat block.

<Day 2 - Precipitation>

○ Materials and equipment

○ Experimental Method

1. Remove the cap mat and place 25 ul of PM1 in each well containing the sample.

2. Cover the cap mat and centrifuge at 1600 rpm for 1 minute.

3. React at 37 ° C for 5 minutes.

4. Remove the plate and centrifuge at 50 × g for 1 min.

5. Remove the cap mat and place 155 ul of 2-propanol in each well containing the sample.

6. Cover the plate using a new cap mat, turn over 10 times, mix and store at 4 ° C for 30 minutes.

7. Centrifuge at 4 ° C at 3,000 rpm for 20 minutes and immediately remove the MSA3 plate from the centrifuge.

8. Immediately remove the cap mat and turn it over quickly to discard the supernatant.

9. Tap on the absorbent pad (kitchen towel, kim towel, etc.) 10 times.

10. Put the inverted plate on the tube rack and dry naturally for 1 hour.

<Day 2 - Resuspend >

○ Materials and equipment

○ Experimental Method

1. Place 23ul of RA1 in each well containing DNA pellet and store the remaining RA1 for XStain HD Bead Chip (freeze storage).

2. Place the foil seal on the MSA3 plate and seal it by pressing the heat-sealer block for 5 seconds.

3. React for 1 hour in an Illumina Hybridization oven at 48 ° C

4. Vortex for 1 minute at 1800 rpm.

5. Centrifuge at 280 × g for 1 minute.

<Day 2 - Hybridization>

○ Materials and equipment

○ Experimental Method

1. MSA3 plate is denatured for 20 minutes at 95 ℃ heat block.

2. After 20 minutes, remove the MSA3 plate from the heat block and allow to cool to room temperature for 30 minutes.

3. Insert the HybChamber Gaskets into the HybChamber as the plate is nearly 30 minutes cool.

4. Place 400ul of PB2 in 8 humidifying buffer reservoirs in HybChamber, close HybChamber lid and place at room temperature.

5. After cooling the DNA for 30 min at room temperature, centrifuge the MSA3 plate at 280 × g for 1 min.

6. Take the stored chips one by one from the refrigerator to get the chips guarantee, align the barcode of the HybChamber insert with the bar code of the chips, and load the samples on both sides of the chips after 15ul per sample using a multi-channel pipette.

7. As soon as the sample loading of each chip is finished, place it in the HybChamber and repeat the following chips in the same way.

8. When the chamber is filled, close the chamber lid and place in the Illumina Hybridization oven at 48 ° C. Set it to speed 5 and react for 16-24 hours.

<Day 3 - Washing bead chips>

○ Materials and equipment

1. Reagents

2. Organization

- Multi-sample beadChip Alignment fixture

- Te-Flow Flow-Through chambers (black frames, spacers, glass back plate and clamps)

- Wash Dish

- Wash Rack

○ Experimental Method

1. Remove the Hyb chamber from the Hybridization oven.

2. Open the lock of the Hyb chamber and take out one insert at a time in the chamber.

3. Pull the seal on the chip to remove it from the chip.

4. Insert the chip with the seal removed into the Wash Rack and dip into the WB1 Wash dish.

5. Once all chips are in the WB1, wash the Wash Rack in a dish for 1 minute, transfer the Wash Rack to another Wash Dish containing PB1 and repeat this process for 1 minute.

6. Immerse again in PB1 wash dish, wash Wash Rack in dish for 1 minute, transfer Wash Rack to another Wash Dish containing PB1 and repeat this process for 1 minute.

7. After washing, place the back frame on the BeadChips Alignment fixture, place the chip one by one in the direction of the barcode, and then fit the space of the transparent part separated from the white part to the top and bottom of the alignment fixture.

8. Raise the space, place the Alignmet bar on the top part of the chip (no barcode), cover the glass with the end of the glass facing the bar, and insert the clip.

(Flow-through chamber assembly completed)

9. After inserting the clip, remove the alignment bar and cut off the space at both ends of the flow-through chamber assembly with scissors.

<Day 3 - XStain Beadchips >

○ Materials and equipment

1. Reagents

2. Organization

- Water circulator

- Chamber Rack

- 2 wash dishes, staining rack, tube rack

○ Experimental Method

1. When the chamber temperature reaches 44 ° C, insert the flow-through chamber assembly into the chamber rack.

2. Add 150ul of RA1 to each chips and let it react for 30 seconds. Repeat this process five more times.

3. Add 450ul of XC1 to each chip and incubate for 10 minutes.

4. Add 450ul of XC2 to each chip and incubate for 10 minutes.

5. Add 200 ul of TEM to each chip and incubate for 10 minutes.

6. Add 450 μl of 95% formamide / 1 mM EDTA to each chip and incubate for 1 minute.

7. React for 5 minutes.

8. Check the temperature on the label of the LTM tube and change the temperature of the chamber rack according to the temperature.

9. Add 450ul of XC3 to each chip and allow to react for 1 minute, then insert again and wait until the temperature reaches 8 times.

10. Add 250 ul of LTM to each chip and let it react for 10 minutes.

11. Add 450ul of XC3, add one more time after 1 minute, and react for 5 minutes.

12. Add 250 ul of ATM to each chip and let it react for 10 minutes.

13. Add 450ul of XC3, add one more time after 1 minute, and react for 5 minutes.

14. Add 250 ul of LTM to each chip and let it react for 10 minutes.

15. Add 450ul of XC3, add one more time after 1 minute, and react for 5 minutes.

Add 250ul of ATM to each chip and let it react for 10 minutes.

17. Add 450ul of XC3, add one more time after 1 minute, and react for 5 minutes.

18. Add 250 ul of LTM to each chip and react for 10 minutes.

19. Add 450ul of XC3, add one more time after 1 minute, and react for 5 minutes.

20. At the end of this process, immediately remove the chamber rack from the flow-through chamber, move it to a room temperature laboratory table and place it flat.

21. Place 310 ml of PB1 in the wash container and immerse the dyeing rack in the container.

22. Using a tool, remove the clips from the chamber rack, lift the glass block, and remove the space on both ends of the bead of chips.

23. Once all attachments on the chip have been removed, immerse them in PB1 of the staining rack contained in PB1. Process all chips in the same way.

24. Slowly douse the chip by moving the dye rack up or down about 10 times and immerse for 5 minutes.

25. Fill 310 ml of XC4 in another flushing vessel, quench 10 times in the same manner as 24, and soak for 5 minutes.

26. After 5 minutes, remove the dyeing rack from the cleaning container and place it on the tube rack as shown in the following figure.

27. Using the forceps, carefully remove the chips from the rack and place them on the tube rack.

28. Carefully put the tube rack on the Chips into a vacuum dryer and allow to dry for 55-55 minutes under a vacuum of 508 mm Hg (0.68 bar).

29. Once the chips are dried, use a kim wipe moistened with ethanol to close the edges of the chip. Be careful not to touch the bead.

30. Beadchips allows you to image with a Scanner within 72 hours after completion of the experiment.

A total of 12 SNPs were selected for high density SNP 170K chip analysis for 50 dogs. The information on these SNPs was as shown in Table 1 below.

The present invention can be used as a genetic marker which can predict the height of dog height early by selecting 12 SNPs significantly correlated with height of dog height by high density SNP 170K chip analysis and genotyping using these SNPs. As an expected effect, it can meet the preference of dog lovers for the height of dogs. By restricting indiscriminate breeding to produce dogs with high or low height of dogs, By identifying genes related to the height of the dog, academic value and basic medical information on the growth of animals can be obtained.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

<110> RURAL DEVELOPMENT ADMINISTRATION <120> SNP marker for prediction of dog's body height and prediction method using the same <130> NPF-28990 <160> 12 <170> PatentIn version 3.2 <210> 1 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 1 aggtctaaat atccttgggt aaataagtag ttttccagat taagatttaa cgaagtaagg 60 aaagtctgtg acagaataca acagttttaa ctcagctcaa agactctcct taagggtccc 120 a 121 <210> 2 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 2 tgtgcaggta tctagaataa cgttgacctg ccaactggat ggccatcctg gtctcctctt 60 atcactgtgc cggaagtata tgttacacct tgcggaaaag agaaacattt aattttttaa 120 a 121 <210> 3 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 3 ggccactacc aaatggaagg ataatgagtg aygctgtgga ttcagggccc tagccgggtc 60 atggagcaag aatgcctgtt gatgtgcaca ctcaagaaag ctgatgctag aaaggggctc 120 a 121 <210> 4 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 4 tatgaagtca tttattaatg agaaatagtt tgccaagatk tttattrgta agttacagat 60 atacagagaa aggagtaaca aagtgcagtc tactgccaaa aatatgaagt gagactttta 120 a 121 <210> 5 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 5 cttcctgtac gccttcttag taatcggtac ccttagggac cttatgacag acgtgcctaa 60 acacaaaacc agagcagctc gtcgctacat tttaaaaaca gagtcaaagt tggaaaaaga 120 t 121 <210> 6 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 6 tgacagtttt gaacgaaata cctatgtacc tgcctgcctt cgtttcggat gcattcttca 60 actcctcact gtgtgtgtca gttccatcca grtggccttt gaactcaacc tcagttgaag 120 t 121 <210> 7 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 7 aatgagggac agttatctgg gctcaatacc tctccctgat aaactggtaa tctacaaaaa 60 ataaaactag aaaaaatctg tgttagcaag actgaaacag ttctcagcca tcctgtgtca 120 a 121 <210> 8 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 8 tcttttctcc tatttagaaa tccacataaa tcatcaaaat tttcatttga gttcaccacc 60 attgtctgaa tttgtatggt aacttgaggc agatgttgcc aaggtgattg ccaaacaaat 120 g 121 <210> 9 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 9 gccttaatgt gttaattcat gggatgctca cctttttgtg ygtatgtaga agatacggat 60 agagagtgag ggtgggggag aaaaaaaaga tgttcttcat caattgcctg tatgtattaa 120 a 121 <210> 10 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 10 gctgttttag aaaggaacca tagcatttcc cattgcttta taaaatatct aaaaatgtgt 60 agttgatggc gtgtgagaca gtcactatct gttcctattc ctcttgagtc agtcctggtg 120 a 121 <210> 11 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 11 ctcctttttg cctatcacta cttaatcagt aaccaagaaa acagaatacc actaccccag 60 atttaatgcc ataccctctc ccagctagta acacattcat ttaagttaag ctttaaagac 120 a 121 <210> 12 <211> 121 <212> DNA <213> Canis lupus familiaris <400> 12 atccaattca aaattgtctg tagaataaaa ggaggactac agaacaaaaa gcaattttcc 60 agaaaatgaa aggagttcat attttgggat gagaggttac ctgtagagag tctaggacag 120 c 121

Claims

A polynucleotide having the 61st base of A or G in the polynucleotide shown in SEQ ID NO: 5 and consisting of 5 to 121 consecutive bases including the 61st base as the base sequence of SEQ ID NO: 5, or a complementary Polynucleotides;
A polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or C in the polynucleotide represented by SEQ ID NO: 7, and the 61rd base is the internal nucleotide sequence of SEQ ID NO: 7, or a complementary Polynucleotides;
A polynucleotide having a 61st base of A or G in the polynucleotide of SEQ ID NO: 8 and consisting of 5 to 121 consecutive bases including the 61st base as the base sequence of SEQ ID NO: 8, or a complementary Polynucleotides;
A polynucleotide having a 61st base of A or G in the polynucleotide of SEQ ID NO: 9 and consisting of 5 to 121 consecutive bases including the 61st base as the base sequence of SEQ ID NO: 9, or a complementary Polynucleotides;
The polynucleotide having the 61st base of A or G in the polynucleotide shown in SEQ ID NO: 11, the polynucleotide consisting of 5 to 121 consecutive bases containing the 61st base as the base sequence of SEQ ID NO: 11, or a complementary Polynucleotides; And
A polynucleotide having a 61st base of A or G in the polynucleotide of SEQ ID NO: 12 and consisting of 5 to 121 consecutive bases containing the 61st base as the base sequence of SEQ ID NO: 12, or a complementary A polynucleotide according to any one of claims 1 to 3, wherein the polynucleotide is a polynucleotide.

A polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide represented by SEQ ID NO: 1, and the 61rd base is an internal nucleotide sequence of SEQ ID NO: 1, or a complementary Polynucleotides;
A polynucleotide having a 61st base of A or G in the polynucleotide represented by SEQ ID NO: 2 and consisting of 5 to 121 consecutive bases containing the 61st base as the nucleotide sequence of SEQ ID NO: 2, or a complementary Polynucleotides;
A polynucleotide having a 61st base of A or G in the polynucleotide represented by SEQ ID NO: 3 and consisting of 5 to 121 consecutive bases including the 61st base as the nucleotide sequence of SEQ ID NO: 3, or a complementary Polynucleotides;
A polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide represented by SEQ ID NO: 4, and the 61rd base is the internal nucleotide sequence of SEQ ID NO: 4, or a complementary Polynucleotides;
A polynucleotide consisting of 5 to 121 consecutive bases, wherein the 61st base is A or G in the polynucleotide shown in SEQ ID NO: 6 and the base sequence of SEQ ID NO: 6 is the 61st base, or a complementary Polynucleotides; And
The polynucleotide having the 61st base of A or G in the polynucleotide shown in SEQ ID NO: 10, the polynucleotide consisting of 5 to 121 consecutive bases including the 61st base as the base sequence of SEQ ID NO: 10, A polynucleotide; and a polynucleotide. The SNP marker composition according to claim 1,

The method according to claim 1,
And an agent capable of detecting or amplifying the dog height height predicting SNP marker.

A kit for estimating height of height of a dog comprising the composition according to any one of claims 1 to 3.

4. A microarray for height-height prediction of dogs comprising the composition of any one of claims 1 to 3.

a) separating the nucleic acid molecule from the dog; And
b) identifying the base type of the SNP corresponding to the 61st base of the polynucleotide of SEQ ID NOS: 5, 7, 8, 9, 11, and 12 in the separated nucleic acid molecule.

The method according to claim 6,
Wherein step (b) comprises amplifying and identifying the isolated nucleic acid molecule.