KR101805450B1 - Structure for preventing microorganism attachment and manufacturing method thereof - Google Patents

Abstract

A nanostructure comprising a plurality of protruding structures with sharp ends, the nanostructure comprising a resin composition; And nano metal particles distributed in the nanostructure. A method of manufacturing a microbe attachment preventive structure includes the steps of preparing a liquid resin (S100); Mixing the nano metal particles with the liquid resin (S200); Applying the liquid resin to the substrate (S300); (S400) pressurizing the liquid resin with a master template in which a pattern corresponding to a plurality of protruding structures is formed; And curing the phase resin (S600).

Description

TECHNICAL FIELD [0001] The present invention relates to a structure for preventing adhesion of microorganisms and a method of manufacturing the same,

The present invention relates to a structure for preventing microbial adhesion and a method for manufacturing the same, and more particularly, to a microbial adhesion preventive structure capable of preventing microbes from adhering to a surface of an object and using the nanomatrix and nano- .

In general, microorganisms float alone and rarely survive. Most of the microorganisms form a three-dimensional structure between the microorganisms by their own polymer material, which is called a biofilm. Microbial biofilms can be formed in almost all types of solid surfaces and in living organisms.

FIG. 1 shows a process in which a microorganism attaches to a surface of a solid to form a biofilm. When microorganisms suspended in the air are attached to the surface of the solid, the microorganisms secrete the polymer material to form a biofilm. When the growth of microorganisms is continued, the microorganisms grow at a certain moment and part of the microorganisms separate from each other and float in the air.

In particular, pathogens can form biofilms in medical devices such as catheters, implanted implants, and artificial organs, and furthermore, can be used in all kinds of artificial facilities accessible to microorganisms such as water pipes, sewage pipes, water purifiers, Preventing the formation of biofilms is one of the concerns that has long been addressed in various technical fields such as civil engineering, architecture, urban and environmental engineering, as well as medical fields, since Edo microorganisms can form biofilms.

Therefore, in order to prevent the growth of microorganisms, it is necessary to prevent the microorganisms from adhering to the surface of the solid to form a biofilm. For this purpose, a technique of forming fine patterns on the surface has been known. FIG. 2 is a cross-sectional view showing a fine pattern according to Patent Document 1, in which a synthetic polymer film 34A is formed on a base film 42A, and a plurality of tip portions 34Ap are formed on the synthetic polymer film 34A . The tip portion 34Ap of the above-described structure is formed in the form of a sharp protrusion, and the tip portion 34Ap destroys the cell wall of the microorganism or the bacteria, thereby preventing the microorganism from adhering to the surface and proliferating.

As another microbial growth preventing technique, there is known a technique of coating nanoparticles of a metal such as copper or silver on the surface of a solid. It is known that when copper or silver nanoparticles penetrate into microorganisms, they interfere with microbial metabolism and lead to bactericidal effects. In order to form or coat the above metal nanoparticles on the surface of a solid, deposition techniques such as sputtering or ion plating are generally used.

As described above, various techniques for preventing the microorganisms from adhering to the surface and growing have been known, but there has been a limit to obtaining a sufficient sterilizing power only by the metal nanoparticle coating or the microstructure itself having the tip portion.

Furthermore, metal nanoparticles may be coated on the surface by sputtering, or metal nanoparticles formed by reacting a metal precursor with a microporous carrier may be used to form metal nanoparticles in the metal nanoparticle colloid. (Patent Document 2), the above-described conventional metal nanoparticle coating techniques are required to be costly or complicated, leading to an increase in production cost.

[Patent Document 1] European Patent Publication No. 2 979 844 (Feb. [Patent Document 2] JP-A-2009-174031 (Aug.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art, and it is an object of the present invention to provide a microbial adhesion preventive structure which is superior in microbial adhesion prevention effect compared with conventionally used microbial adhesion preventive structure, And a method for producing the same.

The present invention relates to a microorganism adhesion preventive structure for preventing microorganisms from adhering to a surface of an object and proliferating, the nanostructure having a protruding structure which hinders adhesion of microorganisms to the surface, the nanostructure comprising a resin composition; And a plurality of nano-metal particles 120 distributed in the nanostructure.

For reference, the protruding structure that hinders the attachment of microorganisms may be a plurality of sharp-pointed structures with sharp ends. If the tip has a shape that can damage the cell membrane of the microorganisms due to its sharp point, The shape is not limited, but generally has a pyramid or conical shape. Further, the protruding structure may be a sinusoidal structure, a columnar structure, or a U-shaped structure in an inverted form.

The plurality of nano metal particles 120 may be at least one metal selected from copper (Cu), silver (Ag), platinum (Pt), gold (Au), zinc (Zn), and palladium The nanometer-sized metal particles 120 are concentrated on the surface of the nanostructure 130, or the distribution density becomes lower as they enter the surface of the nanostructure 130 inward.

The present invention provides a method of manufacturing a microbial adhesion preventive structure that prevents a microorganism from adhering to and growing on a surface, the method comprising: preparing a liquid resin (S100); Mixing the nano metal particles with the liquid resin (S200); Applying the liquid resin to the substrate (S300); (S400) pressurizing the liquid resin with a master template in which a pattern corresponding to a plurality of protruding structures is formed; And curing the liquid resin (S600).

The method further includes a step (S500) of controlling the arrangement state of the nano-sized metal particles by forming an electric field in the master template between the step (S400) of pressing the master template and the step (S600) of curing the liquid resin can do. Then, after the step (S600) of curing the liquid resin, a step (S700) of post-treating the nanocomposite particles to be exposed to the surface of the cured resin may be included.

The microbial protection structure according to the present invention is formed of a polymer resin which is relatively inexpensive and easy to process, has a protruding shape preventing the microorganisms from adhering to the surface, and nanomaterials are distributed on the surface thereof, It is possible to delay the attachment, and at the same time, the nano-metal particles can penetrate into the cells of the microorganism, thereby having a sterilizing power.

Furthermore, when the nanostructure having the projection shape is a plurality of sharp-pointed structures with sharp ends, the cell membrane of the microorganism is broken by the tip portion, and at the same time, the nanometer metal particles easily penetrate into the cells of the fine water, . ≪ / RTI >

Furthermore, in the present invention, nano metal particles are not produced by a method such as sputtering but are manufactured by a method in which nano metal particles suspended in a polymer resin before curing are electrically induced and guided to the surface of a structure, The cost can be greatly reduced.

1 is a conceptual diagram showing a process in which a microorganism is placed on a surface of a structure to form a biofilm.
2 shows a microbial adhesion preventive structure according to the prior art.
3 is a perspective view of a microbial adhesion preventive structure according to a first embodiment of the present invention.
4 is a side cross-sectional view of the microbial adhesion preventive structure according to the first embodiment of the present invention.
5 is a side cross-sectional view of a microbial adhesion preventive structure according to a second embodiment of the present invention.
FIG. 6 is a flowchart showing a manufacturing process of a microbial adhesion preventive structure according to the present invention.
Fig. 7 shows a state in which nano metal particles are mixed in the liquid resin.
8 shows a state in which a liquid resin is applied to a substrate.
9 shows a state in which a liquid resin coated on a substrate is pressed into a master template having a pattern to form a pattern on the liquid resin.
10 shows a state in which the electric field is applied to the master template to control the distribution of metal nanoparticles mixed in the liquid resin.
11 shows a state in which a solid resin nanostructure is formed by curing the liquid resin.
FIG. 12 shows a state in which the resin material on the surface is removed from the solid structure of FIG. 11 to expose the metal nanoparticles to the outside of the nanostructure.

3 is a perspective view illustrating an embodiment of the microbial adhesion preventive structure according to the first embodiment of the present invention. The microbial adhesion preventive structure consists of a collection of a plurality of pointed structures with sharp ends, which can usually be made in the form of a pyramid or a cone.

In order to maximize the sterilization effect, the embodiment shown in FIG. 3 has a structure in which the microorganism attachment prevention structure is composed of a plurality of sharp-tipped structures having sharp ends, but the microorganisms may adhere to the surface The shape of the protrusion is not particularly limited. For example, a plurality of sinusoidal, columnar or inverted U-shaped structures protruding in a plane may also interfere with the attachment of microorganisms to the surface.

Hereinafter, the present invention will be described with reference to an advanced type structure that provides the most maximized effect. However, the following description is not limited to a protrusion structure (sine wave structure, columnar structure, inverted structure U-shaped structure) of the present invention can be obviously understood by those of ordinary skill in the art.

4 is a cross-sectional view of a microbial adhesion preventive structure according to a first embodiment of the present invention. The microbial adhesion preventive structure 100 is formed on the substrate 200. The microbial adhesion preventive structure 100 includes a nanostructure 130 made of a polymer resin and nanostructured metal particles 120 formed on the surface of the nanostructure 130 ). Where the substrate 200 may be the surface of a device where a microbial anti-adhesion structure needs to be formed.

The nanostructure 130 is formed of a plurality of sharp-pointed structures with sharp ends. The sharpened structures may be generally pyramidal or conical, but they are sharp-pointed, The specific shape of the structure is not limited.

The nanostructure 130 is made of a resin composition for convenience of processing. Preferably, the nanostructure 130 is made of a photo-curing resin composition which is solidified when irradiated with ultraviolet rays while maintaining a liquid state before curing. The photo-curing resin composition generally used may be acrylic or epoxy-based, but the material itself is not limited if it is in a liquid form prior to photo-curing but becomes a solid phase after photo-curing. Further, the nanostructure 130 according to the present invention may be made of a thermosetting resin composition such as a phenol resin and an epoxy resin.

The tip-type structure constituting the nanostructure 130 may vary in size depending on the object to be sterilized, but the distance (pitch: D) between the tips of the vertically adjacent structures, which are generally adjacent to each other ) Is 200-300 nm and the vertical distance (height, H) from the bottom to the tip of the nanostructure 130 is 300-500 nm.

For reference, the higher the height (H) of the structure, the greater the effect. However, it can be determined at a reasonable level (more than twice the width of the projection structure) considering the limitations and costs of nanostructure fabrication technology. The pitch of the nanostructure is preferably designed to be ½ to ⅓ of the size of the microorganism (bacteria).

The metal nanoparticles 120 can be used without limitation for a variety of metals as long as they are known to be effective for sterilization. Generally, the metal nanoparticles 120 are made of copper (Cu), silver (Ag), platinum (Pt) (Zn) and palladium (Pd) nanoparticles are known to be effective. The size of nano-metal particles may vary depending on the target to be sterilized.

According to the first embodiment of the present invention, the microorganisms approaching the microbial adhesion preventive structure 100 can prevent the nanomaterial particles 120 present on the surface of the microbial adhesion preventive structure 100 from penetrating into microbes, In this case, when the leading end of the nanostructure 130 damages the cell membrane of the microorganism, the sterilizing effect is amplified, and the increased effect can be obtained as compared with the case where only the nanostructure or the nanomaterial is present.

5 shows a cross section of the microbial adhesion preventive structure 100 according to the second embodiment. In the first embodiment, the nano metal particles 120 are gathered on the surface of the nano structure 130, whereas the nano metal particles 120 are dispersed in the nano metal particles 120 in the first embodiment. The particles 120 are distributed to the inside of the nanostructure 130.

Generally, in terms of cost-effectiveness, it is preferable that all of the nano-metal particles 120 are concentrated on the surface of the nanostructure 130. However, when the micro-organism attachment preventive structure 100 is to be used in an environment in which it is difficult to replace the nanomaterial 130, nanomaterial particles on the surface of the nanostructure 130 may be lost due to abrasion or the like due to long- It is possible to maintain the sterilizing function in place of the nano metal particles in which the metal nanoparticles 120 existing inside the surface are lost. In this case, the metal nanoparticles 120 may be uniformly distributed on the inner surface of the nanostructure 130, but preferably have the highest distribution density on the surface of the nanostructure 130, The distribution density of the metal nanoparticles 120 may be lowered.

FIG. 6 is a flowchart illustrating a process for manufacturing a microorganism attachment prevention structure according to the present invention, and FIGS. 7 to 12 illustrate processes corresponding to the respective steps of the flowchart.

Referring to FIG. 7, a liquid phase resin is prepared (S100) and a liquid phase resin is mixed with a nanometer metal particle (S200). The liquid phase resin is preferably a photo-curable resin, May be used. Although it is possible to distribute nano metal particles evenly in a liquid resin, it may help the subsequent process. However, since the nano metal particles are redistributed in the liquid resin due to the electric field, uniformity is not a big problem.

8 shows a step S300 of applying the liquid resin 300 mixed with the nano-metal particles prepared by the step S200 onto the substrate 400. [ Here, the substrate 400 is a manufacturing tool temporarily used in the process of manufacturing the microbial adhesion preventive structure, and is different from the substrate 200 described above.

9 shows a step 400 of placing a master template 500 on a liquid resin 300 applied on a substrate 400 and pressing the resin 300 on the liquid resin 300. The surface of the master template 500 A pattern corresponding to the nanostructure 130 including the apical shape structure is formed and the surface shape of the liquid resin 300 in the shape corresponding to the above pattern is formed.

10 shows a step S500 of applying an electric field to the master template 500. Preferably, when a positive electric field is applied, the nano metal particles 120 distributed in the resin 300 of the liquid phase are attracted to the electric field And moves to the portion where the master template 500 and the liquid resin 300 are in contact with each other. Therefore, by controlling the strength of the electric field and the time for applying the electric field, the distribution of the nano metal particles 120 in the liquid resin 300 can be controlled.

FIG. 11 shows a step (S600) of thermosetting or photo-curing a liquid resin. When the resin is subjected to a curing process, the resin having fluidity loses its fluidity and solidifies to form the nanostructure 120.

12 shows a step S700 of post-treating the patterned surface of the cured resin, in which the nano metal particles 120 present near the surface of the nanostructure 120 are exposed to the outside of the nanostructure 120 A thin resin film covering the nano metal particles 120 is removed. Although the above process can be performed by blasting, if the thin resin film can be removed, the specific method is not limited.

The microbial adhesion preventive structure manufactured by the above-described method can form a biofilm in a medical instrument such as a catheter, various implants and artificial organs, and furthermore, a microorganism such as a water pipe, a water pipe, It can be applied to all kinds of artificial facilities.

100: microorganism adhesion preventive structure 120: nano metal particle
130: nano structure 200: substrate
300: liquid resin 400: substrate
500: Master template

Claims (9)

The present invention relates to a structure for preventing microorganisms from adhering to the surface of an object and preventing the microorganisms from propagating,
A nanostructure (130) comprising a plurality of projection structures for preventing adhesion of microorganisms, the resin structure being composed of a resin composition; And
And a plurality of nano metal particles 120 distributed in the nanostructure,
Wherein the nanostructured metal particles (120) are arranged in a biased state toward the surface of the nanostructure (130) by being controlled by an electric field.
The microbial adhesion preventive structure according to claim 1, wherein the protruding structure is a plurality of pointed structures having sharp ends.
[Claim 2] The anti-microbial structure according to claim 1, wherein the protrusion structure is one selected from the group consisting of a sine wave structure, a columnar structure, and an inverted U-shaped structure.
The method of claim 1, wherein the plurality of nano metal particles 120 are at least one metal selected from the group consisting of copper (Cu), silver (Ag), platinum (Pt), gold (Au), zinc (Zn), and palladium Wherein the microbial adhesion preventive structure is a microbial adhesion preventive structure.
The anti-microbial structure of claim 1, wherein the plurality of nano metal particles (120) are distributed on a surface (130) of the nanostructure.
The structure according to claim 1, wherein the plurality of nano metal particles (120) are distributed in the nanostructure (130), and the distribution density decreases as they enter the surface from the surface.
The present invention relates to a method of manufacturing a microorganism adhesion preventive structure that prevents microorganisms from adhering to a surface and proliferating,
Preparing a liquid resin (S100);
Mixing the nano metal particles with the liquid resin (S200);
Applying the liquid resin to the substrate (S300);
(S400) pressurizing the liquid resin with a master template in which a pattern corresponding to a plurality of protruding structures is formed;
Forming an electric field in the master template to control an arrangement state of the nano metal particles (S500); And
And curing the liquid resin (S600).
delete The method according to claim 7, further comprising a step (S700) of post-treating the nanocomposite particles so as to be exposed to the surface of the cured resin after the step (S600) of curing the liquid resin .

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