KR101796920B1 - Fluidic Chip integrated cuvette and spectrophotometer with the same - Google Patents

Abstract

The present invention relates to a fluid-chip-integrated cuvette and a spectroscopic analysis apparatus including the same and, more specifically, relates to a fluid-chip-integrated cuvette and a spectroscopic analysis apparatus including the same which aim to facilitate an analysis of generation between a nanostructure of a fluid chip and a liquid sample using a spectroscopic analysis apparatus to measure a degree of transmission or absorption of light with respect to the liquid sample in accordance with wavelength. More specifically, the fluid-chip-integrated cuvette of the present invention relates to a cuvette which is a container where a liquid sample of the analysis apparatus using spectrometry is placed in which the fluid-chip-integrated cuvette includes a nanostructure formed on a surface of a substrate in which the nanostructure includes a nanopattern portion arranged in contact with the liquid sample contained in the cuvette.

Description

TECHNICAL FIELD [0001] The present invention relates to a fluid cube integrated cuvette and a spectrometer including the fluid cube.

[0001] The present invention relates to a fluid cube-integrated cuvette and a spectroscopic analysis apparatus including the same, and more particularly, to a spectroscopic analysis apparatus comprising a fluidic chip- The present invention relates to a fluid-chip-integrated cuvette and a spectroscopic analysis apparatus including the fluid-chip-integrated cuvette for easily detecting or analyzing various existing biological / chemical nanoparticles.

Spectrophotometry is a method of measuring the degree of transmission or absorption of light for a liquid sample according to wavelength.

Sensing using localized surface plasmon resonance (LSPR) also uses spectrophotometry, which is a change in the transmittance of a specific wavelength of light generated from metal nanoparticles present in a liquid sample or a metal nanostructure present on a specific surface .

This method is used to detect the presence or absence of a biomaterial or chemical substance present in a liquid sample by measuring a change in interaction of the liquid sample with the metal particle or the metal nanostructure through spectrum analysis.

The measurement using the method as described above is performed in a solution environment using a spectrophotometer, wherein the liquid sample is generally contained in a cuvette of a spectrophotometer.

FIG. 1 and FIG. 2 are conceptual diagrams of a conventional spectrophotometer using a cuvette, in which a liquid sample to be measured is contained in a cuvette C and measured.

Recently, in order to improve the measurement of local surface plasmon resonance, after forming a metal nanostructure on a substrate surface by using a nano patterning (lithography) technique, various biological / chemical particles existing in a liquid sample are mixed with a nanostructure Through interaction, we make minute changes in the nanostructures and observe the spectral changes resulting therefrom.

With the related art, a multi-nanogap is formed in Korean Patent Laid-Open Publication No. 2016-0037371 (name: a substrate including a plurality of nanogaps and a manufacturing method thereof, publication date: 2016.04.06), and an improved plasmon resonance effect A substrate including a plurality of nanogaps that can be obtained and a method of providing the same are disclosed.

In addition, the development of microfluidic devices and the like has also been performed using a minute amount of fluid.

The microfluidic chip has a function of flowing the fluid through the microfluidic channel and simultaneously performing various experimental conditions.

Specifically, a microchannel is made by using a substrate (or a chip material) such as plastic, glass, or silicon, and the fluid is moved through the channel. After that, sample separation, cell mixing, synthesis, , And cell proliferation can be observed.

Microfluidic chips are also referred to as "lab-on-a-chips " in that they perform experiments that were conventionally performed in the laboratory in small chips.

3 is a diagram illustrating a process of manufacturing a conventional microfluidic chip 10. The finally formed microfluidic chip 10 includes a substrate 11 on which a pattern is formed, a channel 12 for forming a microfluidic channel, (13) and a fitting (14).

However, in the microfluidic chip having the nanostructure forming the pattern, it is difficult to observe the spectral change using the spectrophotometer according to the geometrical limitation. That is, the spectrophotometer is measured by using a cuvette. Since the microfluid chip has a shape different from that of the cuvette, there is a problem that it is troublesome to use a separately manufactured spectrophotometer or to modify a conventional spectrophotometer. In this case, There is a disadvantage that the accuracy is lowered.

Patent Document 1) Korean Laid-Open Patent Publication No. 2016-0037371 (Title: Substrate Containing Multiple Nano Gaps and a Manufacturing Method Thereof, Publication Date: 2016.04.06) Patent Document 2) Korean Laid-Open Patent Publication No. 2016-0038207 (Name: Substrate having a plurality of nanogaps formed thereon and a method for manufacturing the same, published on Jun.

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and it is an object of the present invention to provide a spectroscopic analyzer, which is a device for measuring the degree of transmission or absorption of light with respect to a liquid sample, A fluidic chip-integrated cuvette in which a fluid chip is manufactured in the form of a cuvette used in a spectroscopic analysis apparatus so that particles can be easily analyzed, and a spectroscopic analysis apparatus including the same.

A fluid cube-integrated cuvette according to an embodiment of the present invention is a cuvette for receiving a liquid sample containing nanoparticles, the cuvette including a substrate and a nanostructure formed on the substrate surface, the nanoparticle for capturing the nanoparticle; And a micro channel part in which the nanopattern is mounted so that the liquid sample and the nanostructure are in contact with each other; And a control unit.

In addition, the nano pattern part according to the embodiment of the present invention can be manufactured from a material through which light passes.

In addition, the fluidic cube-integrated cuvette according to the embodiment of the present invention may have a window disposed on one side in the width direction and the nanopattern on the other side.

Further, it may include an inlet and an outlet of the fluid formed on the upper side in the height direction of the micro channel portion according to the embodiment of the present invention.

Also, the micro channel portion according to the embodiment of the present invention may be formed with a channel through which the liquid sample introduced through the inlet port is discharged to the outlet port.

In addition, the micro channel portion according to the embodiment of the present invention may be a hole formed on the flow path, a pattern attaching hole may be formed at a position opposite to the opposite side in the width direction, and a pattern attaching hole And the nano pattern portion or window may be coupled to the pattern attaching hole formed on the other surface.

In addition, the nano pattern part or window according to an embodiment of the present invention may be detachably attached to the pattern attaching hole.

In addition, when the nanotapp parts are attached to the pattern attaching holes formed on both sides in the width direction of the micro channel part according to the embodiment of the present invention, the shape or arrangement of the nanoparticles attached to both sides may be different from each other.

In addition, the fluidic cube-integrated cuvette according to the embodiment of the present invention may have a distance from the bottom surface to the center line of the pattern attaching hole of 15 mm or 8.5 mm.

In addition, if the fluidic cube-integrated cuvette according to the embodiment of the present invention has a distance from the bottom surface to the center line of the pattern attaching hole is 8.5 mm, an adapter of 6.5 mm may be further provided on the bottom side of the micro flow passage.

According to an embodiment of the present invention, the micro channel portion is divided into two regions in the width direction, one of which is a first body portion having an inlet port formed on the upper side thereof and the other is a second body portion having an outlet port formed on the upper side thereof. have.

According to another aspect of the present invention, there is provided a microfluidic channel assembly, comprising: a first fitting part having an inlet port of the first body part formed in a lower depth direction; A second fitting part formed with an outlet of the second body part being embedded in a lower depth direction; A channel part formed between the first body part and the second body part to form a flow path of the liquid sample connecting the end of the first fitting part and the end of the second fitting part; A first cover part coupled to the outside of the nano pattern part or window attached to the first pattern attaching hole, the area corresponding to the first pattern attaching hole formed in the first body part being hollow; A second cover part which is hollowed in a region corresponding to the second pattern attaching hole formed in the second body part and is coupled to the outside of the nano pattern part or window attached to the second pattern attaching hole; . ≪ / RTI >

In addition, the micro channel part according to an embodiment of the present invention is coupled to a surface on which the first pattern attaching hole and the second pattern attaching hole are formed before the nano pattern part or window is attached, And a hollow first sealing member and a second sealing member corresponding to the second pattern attaching hole; As shown in FIG.

In addition, the micro channel portion according to the embodiment of the present invention may include a first body portion and a second body portion in a longitudinal direction of the first body portion and the second body portion, And a second clamper that is coupled to the first body part and the second body part so as to surround and enclose a predetermined area on the other side in the longitudinal direction of the first body part and the second body part.

According to another aspect of the present invention, there is provided a microfluidic channel assembly, comprising: a first fitting part having an inlet port of the first body part formed in a lower depth direction; A second fitting part formed with an outlet of the second body part being embedded in a lower depth direction; A channel part formed integrally with the first body part or the second body part, the flow path of the liquid sample connecting the end of the first fitting part and the end of the second fitting part; A first cover part coupled to the outside of the nano pattern part or window attached to the first pattern attaching hole, the area corresponding to the first pattern attaching hole formed in the first body part being hollow; A second cover part which is hollowed in a region corresponding to the second pattern attaching hole formed in the second body part and is coupled to the outside of the nano pattern part or window attached to the second pattern attaching hole; . ≪ / RTI >

In addition, the micro channel part according to an embodiment of the present invention is coupled to a surface on which the first pattern attaching hole and the second pattern attaching hole are formed before the nano pattern part or window is attached, And a hollow first sealing member and a second sealing member corresponding to the second pattern attaching hole; As shown in FIG.

In addition, the micro channel portion according to an embodiment of the present invention may include the nano pattern portion or window attached to the first pattern attaching hole and the second pattern attaching hole, and the nano pattern portion or window attached between the first cover portion and the second cover portion A third sealing member and a fourth sealing member; As shown in FIG.

In addition, the first and second cover parts may be coupled to each other by bolts and nuts inserted from both sides in the width direction of the micro channel part according to the embodiment of the present invention.

Further, the spectroscopic analyzing apparatus according to the embodiment of the present invention includes a fluidic chip-integrated cuvette for performing a luminous intensity or a spectrophotometric analysis of a liquid sample sample in the cuvette.

In addition, the spectroscopic analyzer according to an embodiment of the present invention includes: a light source for irradiating light in front of a window or a nano pattern part mounted to face the cuvette in a width direction; A detector disposed on a side opposite to the light source with respect to the cuvette and analyzing light incident through the cuvette; And a cuvette holder part for mounting the cuvette between the light source and the detection part; . ≪ / RTI >

The cuvette holder according to the embodiment of the present invention is formed such that the light path through which the light passes is opened so that the light irradiated from the light source passes through all windows or nano pattern parts mounted on both sides in the width direction of the cuvette .

Accordingly, the fluidic chip-integrated cuvette and the spectroscopic analysis apparatus including the fluidic chip of the present invention can easily analyze various biological / chemical particles present in the liquid sample using the nanostructure of the fluidic chip.

That is, according to the present invention, by fabricating a fluid chip in the form of a cuvette used in a spectroscopic analysis apparatus, the spectral analysis apparatus is not required to be modified in order to analyze a spectrum change of light passing through the fluid chip, It is advantageous that the analysis work is simple and the analysis accuracy can be improved.

In addition, when the fluidic chip integrated cuvette according to the embodiment of the present invention forms a fluid chip, the nanopattern portion and the microfluidic channel portion are separately manufactured, so that the fabrication efficiency is very high and mass production is also possible.

In the fluid-cube-integrated cuvette according to the embodiment of the present invention, by attaching the nano-pattern portions to the pattern-attaching holes formed on both sides in the width direction, the detection performance twice as high as when the nano- So that it is possible to perform multiple detection with high sensitivity.

In addition, the present invention is advantageous in that it can be utilized without changing the measurement environment for diagnosis and measurement in various medical and bio fields in the future.

1 and 2 are conceptual diagrams of spectral analysis of a liquid sample using a conventional cuvette.
3 is a flowchart showing a conventional microfluidic chip manufacturing process.
FIG. 4 and FIG. 5 are schematic diagrams of a fluid chip integral type cuvette and spectrum analysis thereof according to an embodiment of the present invention;
6 to 8 are conceptual diagrams of fluid cube-integrated cuvettes according to various embodiments of the present invention.
9 is a conceptual view showing a state in which the nano-pattern part is detached from the fluidic chip-integrated cuvette of FIG.
10 is an exploded perspective view of a fluid cube-integrated cuvette according to the first embodiment;
11 is a front view, a side view, and a rear view showing the fluid-chip-integrated cuvette of Fig.
12 is an exploded perspective view including a nanopattern in the fluidic chip integral cuvette of FIG.
FIG. 13 is a front view, a side view, and a rear view showing a fluidic chip integral type cuvette with a nanopattern attached in the fluidic chip integrated cuvette of FIG. 10;
14 is an exploded perspective view of a fluid cube-integrated cuvette according to a second embodiment;
Fig. 15 is a front view, a side view and a rear view showing the fluid-chip-integrated cuvette of Fig. 14; Fig.
16 is an exploded perspective view including a nanopattern portion in the fluid-chip-integrated cuvette of Fig.
FIG. 17 is a front view, a side view, and a rear view showing a fluidic chip integrated cuvette with a nanopattern attached to the fluidic chip integrated cuvette of FIG. 14;
18 is a view showing a state in which an adapter is mounted on a fluidic cube-integrated cuvette according to an embodiment of the present invention.
19 is a front view showing a general cuvette standard;
20 is a perspective view showing a state in which a fluid chip integral type cuvette is mounted in the spectroscopic analysis apparatus according to the embodiment of the present invention.

Hereinafter, a fluid-cube integrated cuvette according to the present invention as described above and a spectroscopic analysis apparatus including the same will be described with reference to the drawings.

The present invention is for analyzing nanoparticles contained in a liquid sample using a spectroscopic analyzer, which is a device for measuring the degree of light transmission or absorption to a sample according to wavelength. The nanoparticles contained in the liquid sample may include various biological / chemical particles such as metal nanoparticles, biomaterials, and chemicals, and the scope of the present invention is not limited to the specific particles in the liquid sample.

According to the present invention, a nanostructure is used for collecting nanoparticles contained in a liquid sample, and the nanostructure is installed in the cuvette so as to be in contact with the liquid sample, so that the liquid sample Can be detected and analyzed.

FIG. 4 and FIG. 5 conceptually show a spectroscopic analysis apparatus including a fluid-chip-integrated cuvette according to an embodiment of the present invention.

4 and 5, the fluid-chip-integrated cuvette 1 is largely formed by including the nano-pattern portion 100 and the micro channel portion 300.

The nano pattern part 100 includes a substrate 120 and a nanostructure 110 formed on the surface of the substrate 120. Resonance occurs at a specific wavelength of light according to the arrangement pattern and shape of the nanostructure 110 on the substrate 110, and light is absorbed at this wavelength. Accordingly, when the nanoparticles in the liquid sample are collected in the nanostructure 110 and the shape of the nanostructure in which the light is incident is changed, the frequency region where the light is absorbed is changed, and the presence of nanoparticles can be detected.

The nanostructure 110 may be formed in a shape capable of collecting nanoparticles, or a separate receptor may be formed on the nanostructure to collect nanoparticles in the liquid sample.

Next, the micro channel part 300 is formed to receive a liquid sample therein, and the nanopattern part 100 is mounted so that the liquid sample in the micro channel part 300 and the nanostructure 110 are in contact with each other .

In the embodiment shown in FIG. 4, the fluidic chip integral cuvette 1 has a structure in which the micro channel portion is formed in a substantially container shape, and a liquid sample is accommodated therein, a window 130 is disposed on one side in a width direction, And the nano pattern part 100 is disposed on the other side.

At this time, the substrate 120 of the nano pattern part 100 and the window 130 are both made of a material through which the light passes, so that the light source 510 of the spectroscopic analysis apparatus 1000 can be completely passed. As described above, the light source 510 passing through the window 130 and the nano pattern unit 100 is analyzed by the detector 520.

Next, FIG. 5 shows a fluidic chip-integrated cuvette 1 to which a form of a fluid chip having a finer flow path is applied to a cuvette than the embodiment of FIG.

As shown in Fig. 5, the fluidic chip integral cuvette 1 having a fine flow path can be modified in various forms, and an embodiment thereof is shown in Fig. 6 and Fig.

Hereinafter, with reference to FIG. 5 to FIG. 9, the structure of a fluid cube-integrated cuvette according to an embodiment of the present invention will be described in more detail.

6 is a front view (a) of a fluid cube-integrated cuvette shown in Fig. 5, a longitudinal sectional view (b), a side view (c) and a rear view (d) showing a flow path of a liquid sample formed in the micro- FIG. 7 is a front view (a) of the fluidic chip integrated cuvette attached so as to face the nano pattern part 100, a vertical sectional view (b), a side view (c) Figure (d) is shown.

6 and 7 includes a nano pattern part 100, a micro channel part 300, an inlet port 210 and an outlet port 220. The nano pattern part 100,

The micro channel part 300 forms the body of the cuvette, and the overall dimensions are formed to have the same dimensions as those of the cuvette used in the existing spectroscopic analysis apparatus 1000.

The micro channel part 300 is formed with a fine flow path 301 through which the liquid sample flowing through the inlet port 210 is discharged to the outlet port 220.

In addition, in the micro channel part 300, a pattern attaching hole 310 is formed in a hole shape formed on the flow path 301 and at positions opposite to each other in the width direction.

6, the nano pattern part 100 is coupled to the pattern attaching hole 310 formed on one side of both sides in the width direction, and the pattern attaching hole (not shown) formed on the other side of the micro passageway part 300, 310, and the nano pattern portion 100 may be attached to the pattern attaching holes 310 facing each other as shown in FIG.

7, in the nano pattern part 100 attached to both sides of the micro channel part 300, the patterns or the arrangement of the nanostructures 110 forming the pattern may be the same or different from each other, Shape or arrangement.

Accordingly, by attaching the nanopattern portions to the pattern attaching holes formed on both sides in the width direction of the fluidic chip integrated cuvette according to the embodiment of the present invention, the detection performance twice as much as when the nanopattern portions are attached in one place So that it is possible to perform multiple detection with high sensitivity.

Unlike the conventional fluid chip, in which the pattern forming process and the microchannel fabrication process are manufactured through a single process, the fluidic cube-integrated cuvette 1 according to the embodiment of the present invention includes the nano- (100) are separately manufactured, it is possible to configure various materials and patterns, and the pattern forming process and the fine flow path forming process can be separated to improve manufacturing efficiency.

8 is a front view (a) of a fluid cube-integrated cuvette formed so that the nano pattern part 100 is detachably attached thereto, a vertical cross-sectional view (b) and a side view c) and a rear view (d) are shown in FIG. 9. FIG. 9 is a conceptual view showing a state in which the nano pattern part is removed in FIG.

8 and 9, the micro channel part 300 includes a separate first cover part 341 and a second cover part 342, so that the nano pattern part 100 or the window 130 Can be detachably attached.

The first cover portion 341 and the second cover portion 342 may be mechanically coupled to the body portion of the micro flow path portion 300. The shape of the first cover portion 341 and the second cover portion 342 will be described in more detail in the following embodiments do.

In the conventional spectroscopic analysis apparatus 1000, considering the height of the window 130 of the cuvette positioned from the bottom of the cuvette, the fluid-cube integrated cuvette 1 is moved from the bottom to the center line of the pattern attachment hole 310 Is preferably one of 15 mm or 8.5 mm.

Particularly, when the distance between the bottom surface of the cuvette 1 and the center line of the pattern attaching hole 310 is 8.5 mm, a 6.5 mm adapter 400 is attached to the lower side of the micro channel section 300 It is possible to use the spectroscopic analyzer 1000 without modification of the spectroscopic analysis apparatus 1000 (see Figs. 18 and 19).

The channel part 330 for detachably attaching the nanopattern part 100 or the window 130 and forming a flow path of a liquid sample in the micro channel part 300 in the fluid cube integrated cuvette 1, And an example formed by a separate structure will be described.

Example 1.

10 to 13, the present invention relates to a fluidic chip-integrated cuvette 1 in which a channel portion 330 for forming a flow path of a liquid sample in a fine channel portion 300 is formed in a different configuration.

FIG. 10 is an exploded perspective view of the fluidic chip integrated cuvette according to the first embodiment, FIG. 11 is a front view (a), a side view (b) and a rear view (c) 10 is an exploded perspective view including a nano pattern portion in the fluid chip integrated cuvette of Fig. 10, Fig. 13 is a front view (a), a side view (b), and a rear view c).

10 and 11, the fluidic chip-integrated cuvette 1 includes an inlet 210 and an outlet 220 formed on the upper side in the height direction of the cuvette, And a fine channel portion 300 in which a pattern attaching hole 311 and a second pattern attaching hole 312 are formed.

10 and 11, the micro channel part 300 includes a first body part 321, a second body part 322, a first fitting part 211, a second fitting part 211, (221) and a channel portion (330).

The first body portion 321 is one in which the body of the cuvet is divided into two regions in the width direction. The inlet body 210 is formed on the upper side, the second body portion 322 is formed on the upper side, An outlet 220 is formed.

At this time, the inlet 210 and the outlet 220 may be formed in any one of the first body portion 321 and the second body portion 322, but the cuvette It is preferable to arrange them staggered with each other in the width direction, as described above, in consideration of the size of the fitting portion and the size of the commercially available fitting portion.

The first fitting part 211 is formed with an inlet port 210 of the first body part 321 and a second fitting part 222 coupled to the second body part 322 Is formed with a predetermined depth in a downward direction.

11, the first fitting 212 is inserted into the first fitting part 211 and the second fitting 222 is inserted into the second fitting part 221. As shown in FIG.

The channel part 330 has a thin plate shape such as silicon or the like and has a flow path of a liquid sample connecting between the end of the first fitting part 211 and the end of the second fitting part 221, And is mounted between the first body portion 321 and the second body portion 322.

The channel portion 330 may be mechanically fixed to the first body portion 321 and the second body portion 322 by fastening means such as screw fastening or a separate clamper.

12 and 13, the fluidic cube-integrated cuvette 1 further includes a first cover portion 341 and a second cover portion 342, so that the microchannel 300 is provided with the nano- The portion 100 or the window 130 may be detachably attached.

The first cover part 341 is hollow in the area corresponding to the first pattern attaching hole 311 formed in the first body part 321 and the nano pattern part attached to the first pattern attaching hole 311, (100) or the window (130).

The second cover portion 342 is hollow in a region corresponding to the second pattern attaching hole 312 formed in the second body portion 322 and the nano attached to the second pattern attaching hole 312 And is coupled to the outside of the pattern unit 100 or the window 130.

The fluidic cube-integrated cuvette 1 is attached to the surface on which the first pattern attachment hole 311 and the second pattern attachment hole 312 are formed before the nano pattern part 100 or the window 130 is coupled. And a hollow first sealing member 351 and a second sealing member 352 are further mounted in a region corresponding to the first pattern attaching hole 311 and the second pattern attaching hole 312, Can be prevented.

At this time, the first sealing member 351 and the second sealing member 352 are made of elastic material such as silicon or polymer, and have a thin plate shape.

10 and 12, the micro channel portion 300 includes a first sealing member 351 and a second sealing member 352, and a first guide (not shown) between the nanopattern portion 100 381 and a second guide 382 can be further mounted.

The first guide 381 and the second guide 382 include a hole formed in a hollow corresponding to the nano pattern part 100 to guide the position where the nano pattern part 100 is to be attached .

10, the micro channel portion 300 may be formed in the first body portion 321 and the second body portion 322 such that the first body portion 321 and the second body portion 322 are coupled to each other in the width direction. A first clamper 361 which surrounds a predetermined region of the outer surface at one side in the longitudinal direction of the second body portion 322 and a second clamper 362 which surrounds the other side in the longitudinal direction of the first body portion 321 and the second body portion 322, And a second clamper 362 which surrounds the outer circumferential surface of the first clamper.

The first clamper 361 and the second clamper 362 are configured to mechanically couple the first body portion 321 and the second body portion 322. At this time, the first and second clamper 361 and 362 are inserted into the first body part 321 and the second body part 322, respectively, so that the fluidic- A first clamper insertion groove 371 and a second clamper insertion groove 372 may be formed so as to be inserted inward by a corresponding size so as to be inserted and coupled.

Accordingly, in the fluidic cube-integrated cuvette 1 according to the first embodiment, the liquid sample introduced from the inlet 210 flows in the channel portion 330 along the flow path and flows into the pattern attaching hole 310 The attached nano pattern part 100 and the liquid sample are brought into contact with each other so that the light irradiated from the light source 510 passes through the nano pattern part 100 and the liquid sample and is guided to the detection part 520 ), And spectrum analysis is performed.

Example 2.

14 to 17, the channel portion 330 forming the flow path of the liquid sample in the micro channel portion 300 is formed in the first body portion 321 or the second body portion 322 (1) integrally formed with a fluid chip.

Fig. 14 is an exploded perspective view of the fluid cube integrated cuvette according to the second embodiment, Fig. 15 is a front view (a), a side view (b) and a rear view (c) Fig. 17 is a front view (a), a side view (b) and a side view (b) showing a state in which the nanopattern is attached to the fluidic chip-integrated cuvette of Fig. 14 The back view is (c).

Referring to FIG. 14, the fluidic chip integrated cuvette 1 includes an inlet 210 and an outlet 220 formed on the upper side in the height direction of the cuvette, And a fine channel portion 300 having a hole 311 and a second pattern attaching hole 312 formed therein.

The micro channel part 300 includes a first body part 321, a second body part 322, a first fitting part 211, a second fitting part 221, a channel part 330, .

The first body portion 321 is one in which the body of the cuvet is divided into two regions in the width direction. The inlet body 210 is formed on the upper side, the second body portion 322 is formed on the upper side, An outlet 220 is formed.

At this time, the inlet 210 and the outlet 220 may be formed in any one of the first body portion 321 and the second body portion 322, but the cuvette It is preferable to arrange them staggered with each other in the width direction, as described above, in consideration of the size and the size of the commercially available fitting portion.

The first fitting part 211 is formed to have an inlet 210 of the first body part 321 and a second fitting part 221 inserted into the second body part 322 are formed with a predetermined depth in the lower direction.

14 and 15, the first fitting 212 is inserted into the first fitting part 211 and the second fitting 222 is inserted into the second fitting part 221, do.

The channel part 330 is a flow path of a liquid sample that connects the end of the first fitting part 211 and the end of the second fitting part 221 to the first body part 321 or And is integrally formed with the second body portion 322.

17, the fluid-cube integrated cuvette 1 includes a first cover portion 341 and a second cover portion 342, so that the nano-pattern portion (not shown) 100 or the window 130 can be detachably attached.

The first cover part 341 is hollow in the area corresponding to the first pattern attaching hole 311 formed in the first body part 321 and the nano pattern part attached to the first pattern attaching hole 311, (100) or the window (130).

The second cover portion 342 is hollow in a region corresponding to the second pattern attaching hole 312 formed in the second body portion 322 and the nano attached to the second pattern attaching hole 312 And is coupled to the outside of the pattern unit 100 or the window 130.

The first cover part 341 and the second cover part 342 are connected to the first body part 321 and the second body part 322 by the bolts 410 and the nuts 420 inserted at both sides in the width direction, And may be coupled to each other in any other manner.

14 and 16, before the nano pattern part 100 or the window 130 is coupled to the micro channel part 300, the first pattern attaching hole 311 and the second pattern attaching hole 311 The first patterning hole 311 and the second patterning hole 312 are coupled to the surface on which the pattern attaching hole 312 is formed and the region corresponding to the first pattern attaching hole 311 and the second pattern attaching hole 312 is a hollow first sealing member 351, A member 352 may be further mounted to prevent leakage of the liquid sample.

16, the nano pattern part 100 or the window 130 attached to the first pattern attaching hole 311 and the second pattern attaching hole 312 is formed in the micro channel part 300, And a third sealing member 353 and a fourth sealing member 354 mounted between the first cover portion 341 and the second cover portion 342 may be further mounted.

At this time, the first to fourth sealing members 351 to 354 are made of elastic material such as silicon or polymer, and have a thin plate shape.

The fluidic chip-integrated cuvette 1 of the second embodiment has an advantage that it is more compact than the first embodiment and is easier to manufacture.

Example 3.

Embodiment 3 relates to a spectrophotometric apparatus for performing the luminous intensity or spectral luminous intensity analysis of a liquid sample in the cuvette, including the fluidic chip-integrated cuvette 1 of Embodiment 1 or Embodiment 2.

As shown in FIG. 20, the spectrophotometric apparatus includes a fluidic chip-integrated cuvette 1 and a window 130 or nano-pattern unit 100 mounted to face the cuvette in the width direction, A detector 520 disposed on a side opposite to the light source 510 with respect to the cuvette and analyzing light incident through the cuvette 510; And a cuvette holder part 530 for mounting the cuvette between the cuvette holder 520 and the cuvette holder part 530.

At this time, the cuvette holder part 530 is arranged so that the light emitted from the light source 510 passes through the window 130 or the nano pattern part 100 mounted on both sides in the width direction of the cuvette, The light path should be open.

Accordingly, by fabricating a fluid chip in the form of a cuvette used in the spectroscopic analysis apparatus 1000, the spectroscopic analysis apparatus 1000 is not required to be modified in order to analyze a spectrum change of light passing through the fluid chip, Since the fluidic chip-integrated cuvette 1 is simply mounted on the cuvette holder of the spectroscopic analysis apparatus 1000, the analysis work is simple and the analysis accuracy can be improved.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It goes without saying that various modifications can be made.

1: Integrated fluid cube
100: nano pattern part 110: nano structure
120: substrate 130: window
210: inlet
211: first fitting part 212: first fitting
220: Outlet
221: second fitting coupling part 222: second fitting
300: fine flow path portion
301: Euro
310: pattern attaching hole
311: first pattern attaching hole 312: second pattern attaching hole
321: first body part 322: second body part
330:
341: first cover part 342: second cover part
351 to 354: first to fourth sealing members
361: first clamper 362: second clamper
371: first clamper insertion groove 372: second clamper insertion groove
381: first guide 382: second guide
400: Adapter
410: Bolt 420: Nut
510: light source 520:
530: Cuvette holder part
1000: Spectroscopic analyzer

Claims (17)

A cuvette for receiving a liquid sample comprising nanoparticles,
A nanopattern for collecting the nanoparticles, the nanoparticle being formed on a surface of the substrate; And
And a micro channel part in which a space for accommodating the liquid sample is formed and in which the nanoparticle part is mounted so that the liquid sample and the nanostructure are in contact with each other,
The micro-
A flow path is formed in which a liquid sample flowing through an inlet through which the fluid flows is discharged to an outlet through which the fluid flows,
Wherein one of the two regions is divided into two regions in the width direction and one of them forms a first body portion having the inlet port on the upper side and the other one is a second body portion having the outlet port on the upper side thereof.
delete The method according to claim 1,
The fluidic chip integral cuvette
Wherein the window is disposed on one side in the width direction, and the nanopattern is disposed on the other side of the cuvette.
delete The method according to claim 1,
The micro-
A pattern attaching hole is formed at positions opposite to each other in the width direction,
Wherein the nano pattern part is coupled to the pattern attaching hole formed on one side of the both side faces and the nano pattern part or the window is coupled to the pattern attaching hole formed on the other side face.
6. The method of claim 5,
The nano pattern portion or window
And is detachably attached to the pattern attaching hole.
6. The method of claim 5,
The micro-
Wherein the nano-pattern portions attached to both sides of the nano-pattern portion are different in shape or arrangement when the nano-pattern portion is attached to the pattern attaching holes formed on both sides in the width direction.
delete The method according to claim 1,
The micro-
A first fitting engagement portion formed with an inlet port of the first body portion being embedded in a lower depth direction;
A second fitting part formed with an outlet of the second body part being embedded in a lower depth direction;
A channel part formed between the first body part and the second body part to form a flow path of a liquid sample connecting the end of the first fitting part and the end of the second fitting part;
A first cover part coupled to the outside of the nano pattern part or window attached to the first pattern attaching hole, the area corresponding to the first pattern attaching hole formed in the first body part being hollow; And
A second cover portion that is hollowed in a region corresponding to the second pattern attaching hole formed in the second body portion and is coupled to the outside of the nano pattern portion or window attached to the second pattern attaching hole; And a fluid-cushion-integrated cuvette.
10. The method of claim 9,
The micro-
Wherein the first pattern attaching hole and the second pattern attaching hole are bonded to a surface on which the first pattern attaching hole and the second pattern attaching hole are formed before the nano pattern part or window is attached, A first sealing member and a second sealing member; Further comprising: a fluid-cushion-integrated cuvette.
11. The method of claim 10,
The micro-
The first body portion and the second body portion are coupled in the width direction,
A first clamper that is coupled to one side of the first body portion and the second body portion in a longitudinal direction and surrounds a predetermined region of the outer side surface,
And a second clamper which is coupled to the first body part and the second body part so as to surround and enclose a predetermined area on the outer side in the longitudinal direction of the first body part and the second body part.
11. The method of claim 10,
The micro-
A first fitting engagement portion formed with an inlet port of the first body portion being embedded in a lower depth direction;
A second fitting part formed with an outlet of the second body part being embedded in a lower depth direction;
A channel part integrally formed with the first body part or the second body part with a flow path of a liquid sample connecting the end of the first fitting part and the end of the second fitting part;
A first cover part coupled to the outside of the nano pattern part or window attached to the first pattern attaching hole, the area corresponding to the first pattern attaching hole formed in the first body part being hollow; And
A second cover portion that is hollowed in a region corresponding to the second pattern attaching hole formed in the second body portion and is coupled to the outside of the nano pattern portion or window attached to the second pattern attaching hole; And a fluid-cushion-integrated cuvette.
13. The method of claim 12,
The micro-
Wherein the first pattern attaching hole and the second pattern attaching hole are bonded to a surface on which the first pattern attaching hole and the second pattern attaching hole are formed before the nano pattern part or window is attached, A first sealing member and a second sealing member; Further comprising: a fluid-cushion-integrated cuvette.
14. The method of claim 13,
The micro-
A third sealing member and a fourth sealing member mounted between the first cover portion and the second cover portion; Further comprising: a fluid-cushion-integrated cuvette.

Comprising a fluidic chip integral cuvette according to any one of claims 1, 3, 5 to 7 and 9 to 14, wherein the intensity or spectrophotometric analysis of the liquid sample in the cuvette A spectroscopic analyzer for performing the method.
16. The method of claim 15,
The spectroscopic analyzer
A light source for irradiating light in front of a window or nano pattern part mounted to face the cuvette in a width direction;
A detector disposed on a side opposite to the light source with respect to the cuvette and analyzing light incident through the cuvette; And
A cuvette holder part for mounting the cuvette between the light source and the detection part; Wherein the spectroscopic analyzing apparatus comprises:
17. The method of claim 16,
The cuvette holder portion
The light irradiated from the light source passes all the window or nano pattern part mounted on both sides in the width direction of the cuvette,
And an optical path through which light passes is opened.

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