KR101796184B1 - Particle separation apparatus using pressure-driven flow and free-flow electrophoresis - Google Patents

Particle separation apparatus using pressure-driven flow and free-flow electrophoresis Download PDF

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KR101796184B1
KR101796184B1 KR1020150155190A KR20150155190A KR101796184B1 KR 101796184 B1 KR101796184 B1 KR 101796184B1 KR 1020150155190 A KR1020150155190 A KR 1020150155190A KR 20150155190 A KR20150155190 A KR 20150155190A KR 101796184 B1 KR101796184 B1 KR 101796184B1
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particles
flow
electrophoretic mobility
particle
pressure
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KR20170052988A (en
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임근배
전형국
김영규
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포항공과대학교 산학협력단
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L33/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
    • C08L33/04—Homopolymers or copolymers of esters
    • C08L33/06—Homopolymers or copolymers of esters of esters containing only carbon, hydrogen and oxygen, which oxygen atoms are present only as part of the carboxyl radical
    • C08L33/10—Homopolymers or copolymers of methacrylic acid esters
    • C08L33/12—Homopolymers or copolymers of methyl methacrylate
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L69/00—Compositions of polycarbonates; Compositions of derivatives of polycarbonates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

A particle separating apparatus according to an embodiment of the present invention includes a sample channel into which a fluid containing charged particles flows and a particle which flows from the sample channel and mixed with the fluid while the fluid for pressure- And an electrode portion for applying an electric field to the outside of the chamber, wherein the electrode portion includes an anode provided at one end of both ends of the separation channel, Wherein the particles are separated and moved to one end or the other end of the separation channel according to the electrophoretic mobility of the particles when the electric field is applied and the electrophoretic mobility Relatively large particles move in the direction opposite to the direction of the pressure-driven flow, and particles having relatively small electrophoretic mobility move in the direction of the pressure-driven flow It may be configured to move in a direction.

Description

TECHNICAL FIELD [0001] The present invention relates to a particle separating apparatus using pressure-driven flow and free-flow electrophoresis,

The present application relates to a particle separating apparatus.

Recently, studies on separation devices based on free-flow electrophoresis have been actively conducted.

In the free-flow electrophoresis separation method, the moving path is refracted by the electric field applied by the charged particles introduced by the external flow in the direction perpendicular to the flow. At this time, the angle of refraction is changed according to the electrophoretic mobility of the particles It is a method in which particles are separated.

The free-flow electrophoresis separation method has a great advantage in that the particles can be separated continuously with high resolution, but since the electrodes must be installed inside the device, the manufacturing process is complicated and difficult, and a large problem .

According to one embodiment of the present invention, since an electrode is required to be installed in the device, the manufacturing process is complicated and difficult, and the problem of bubbles being generated in the device is solved, and particles capable of continuously separating particles of various sizes Separation device.

According to one embodiment of the present invention, a sample channel into which a fluid containing charged particles is introduced, and a fluid for pressure-driven flow, A chamber having a T-shaped microchannel in which a separation channel is connected in a T-shape; And an electrode part for applying an electric field outside the chamber, wherein the electrode part includes an anode provided at one end of both ends of the separation channel and a cathode provided at the other end of both ends of the separation channel, When the electric field is applied, the particles are separated and moved to one end or the other end of the separation channel according to the electrophoretic mobility of the particles. Particles having a relatively large electrophoretic mobility among the particles, And the particles having a relatively small electrophoretic mobility move in the direction of the pressure-driven flow.

According to an embodiment of the present invention, the electrode portion can separate the particles by adjusting the intensity of the electric field.

delete

delete

According to an embodiment of the present invention, the surface of the T-shaped microchannel may be coated with 1 wt% bovine serum albumin (BSA).

According to one embodiment of the present invention, the particles may be particles having a negatively charged electrophoretic mobility of two sizes.

According to one embodiment of the present invention, the fluid may be a 1-mM DSP (Dibasic Sodium Phosphate) solution having a pH of 7.1.

According to one embodiment of the present invention, the particles may be nanoscale or micro sized particles, including fluorescent dyes (BODIPY 2 - , PTS 4 - ), polystyrene particles, glass particles. The particle separating apparatus described above can also be applied to separation of biomolecules such as cells, proteins, and DNA.

According to an embodiment of the present invention, the T-shaped microchannel is formed on a substrate, and the substrate is made of glass, silicon, polymer plastic, pyrex, silicon dioxide, But not limited to, silicon nitride, quartz, polymethylmethacrylate (PMMA), polycarbonate (PC), acrylic or cyclic olefin copolymer (COC) .

According to one embodiment of the present invention, since an electric field is applied in the direction opposite to the direction of the pressure driving flow outside the T-shaped microchannel to separate the particles according to the electrophoretic mobility of the particles, And it is possible to continuously separate particles of various sizes while solving the problem that bubbles are generated inside the apparatus.

1 is a configuration diagram of a particle separating apparatus according to an embodiment of the present invention.
FIG. 2 is a cut-away plan view of the particle separating apparatus of FIG.
FIG. 3 is a view for explaining the measurement process of electroosmotic flow mobility.
4 is a graph showing the relationship between an applied electric field and particle flow rates of various sizes
FIG. 5 is a graph showing the relationship between the particle size, the electrophoretic mobility, and the electric field intensity of FIG.
FIG. 6 illustrates a process of separating micro-sized particles according to electrophoretic mobility according to an embodiment of the present invention,
7 is a view illustrating a process of separating nano-sized particles according to electrophoretic mobility according to an embodiment of the present invention.

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. However, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below. The shape and the size of the elements in the drawings may be exaggerated for clarity and the same elements are denoted by the same reference numerals in the drawings.

1 is a configuration diagram of a particle separating apparatus according to an embodiment of the present invention. FIG. 2 is a plan view of the particle separator of FIG.

1 and 2, a particle separator according to an embodiment of the present invention includes a substrate 111, a chamber 110 including a T-shaped microchannel 112 formed on the substrate 111, , And an external electrode (113). The T-shaped microchannel 112 may be composed of a sample channel 112a and a separation channel 112b.

The substrate 111 located under the chamber 110 may be formed of a material such as glass, silicon, polymer plastic, pyrex, silicon dioxide, silicon nitride, quartz, (PMMA), a polycarbonate (PC), an acrylic or a cyclic olefin copolymer (COC), and the like.

On the other hand, the T-shaped microchannel 112 may be fabricated on the surface of the substrate 111 using, for example, PDMS (Polydimethylsiloxane) and general photolithography.

The aforementioned T-shaped microchannel 112 includes a sample channel 112a into which a fluid containing charged particles is introduced and a sample channel 112b through which the fluid for pressure-driven flow is introduced and simultaneously flows from the sample channel 112a, And the separation channel 112b in which the separated particles are separated may be connected in a T-shape.

Exemplary diameters and electrophoretic mobility of particles applied according to one embodiment of the present invention are shown in Table 1 below.

[Table 1]

Figure 112015107855130-pat00001

That is, the fluid for pressure-driven flow is introduced into the one end 114a of the separation channel 112b through the first inlet reservoir 114 and the introduced fluid is introduced into the other end 116a of the separation channel 112b And may exit to a connected outflow reservoir 116.

In addition, a fluid comprising charged particles may be introduced through a second inlet reservoir 115 that is thermally coupled to the sample channel 112a. In addition, a sludge pump can be connected to the first inflow passage 114 and the second inflow passage 115, and the fluid can be supplied at a constant flow rate by the operation of the sludge pump.

Here, the particles may be particles having two sizes of electrophoretic mobility negatively charged with nano-sized or micro-sized particles. For example, fluorescent dyes (BODIPY 2 - , PTS 4 - ), polystyrene particles, and glass particles can be exemplified and can be applied to the separation of biomolecules such as cells, proteins and DNA. In addition, the fluid may be a 1-mM DSP (Dibasic Sodium Phosphate) solution having a pH of 7.1.

In this embodiment, the height of the separation channel 112b and the sample channel 112a of the T-shaped microchannel 112 is 45 占 퐉, the separation channel 112b is 600 占 퐉 wide, and the separation channel 112b is 2 cm . The width of the sample channel 112a may be 100 占 퐉 and the length of the sample channel 112a may be 300 占 퐉. It should be noted that the above-described specific numerical values may be changed according to the needs of those skilled in the art.

The surface of the T-shaped microchannel 112 described above may be coated with 1 wt% bovine serum albumin (BSA). The reason for this is that the particle flow rate can be expressed by the sum of the flow rate by the pressure drive flow, the flow rate by the electroosmotic flow, and the flow rate by the electrophoresis. By coating the surface of the T-shaped microchannel 112 with 1 wt% , To exclude the influence of the flow rate due to the electroosmotic flow.

Meanwhile, the electrode unit 113 can separate particles by applying an electric field outside the chamber 110 and adjusting the intensity of the applied electric field. The electrode unit 113 described above includes a positive electrode 113a provided on one end 114a side of the separation channel 112b and a negative electrode 113b provided on the other end 116a side of the separation channel 112b Lt; / RTI >

Particles having a relatively high electrophoretic mobility among the particles are one end 114a of the separation channel 112b and particles having a relatively small electrophoretic mobility among the particles are separated from the other end 116a of the separation channel 112b ) To separate the particles.

Hereinafter, the operation principle of the particle separating apparatus according to one embodiment of the present invention will be described with reference to Fig.

1 and 2, the basic principle of the present invention is that the particle-free fluid is introduced at a constant flow rate through the inlet passageway 114 of the separation channel 112b and the inlet passageway 114 of the sample channel 112a The fluid containing the particles P1 and P2 is introduced through the fluid passage 115. Here, the particles (P1, P2) are negatively charged particles, and may be particles having electrophoretic mobility of two sizes. The anode 113a may be disposed at one end 114a of the separation channel 112b and the cathode 113b may be disposed at the other end 116a of the separation channel 112b. The electrophoretic mobility is assumed to be larger than particle P2.

First, in a state in which no electric field is applied by the electrode unit 113, the fluid introduced from one end 114a of the separation channel 112b flows by a pressure-driven flow (PDF), and the sample channel 112a The particles P1 and P2 introduced through the inlet passages 115 of the separation channels 112b flow to the other end 114b of the separation channel 112b.

However, when a voltage is applied by the electrode unit 113, the particles P1 and P2 introduced through the inlet channel 115 of the sample channel 112a can be separated according to the electrophoretic mobility.

Particularly, the particles P1 having a large electrophoretic mobility move over the force by the pressure drive flow PDF and move to the end 114a of the separation channel 112b by electrophoresis (EP) Particles P2 having a relatively small degree of migration can migrate to the other end 114b of the separation channel 112b without overcoming the force due to the pressure drive flow PDF, thereby separating the particles according to the electrophoretic mobility .

As described above, the particle flow rate can be represented by the sum of the flow rate due to the pressure-driven flow, the flow rate due to the electroosmotic flow, and the flow rate due to the electrophoresis. In order to separate the particles, This will be described in detail.

The particle flow rate in the T-shaped microchannel (

Figure 112015107855130-pat00002
) Can be expressed by the following equation (1).

[Equation 1]

Figure 112015107855130-pat00003

here,

Figure 112015107855130-pat00004
The particle flow rate,
Figure 112015107855130-pat00005
The particle flow rate due to the pressure-driven flow,
Figure 112015107855130-pat00006
Lt; RTI ID = 0.0 > electroosmotic &
Figure 112015107855130-pat00007
An electric field,
Figure 112015107855130-pat00008
Is an electrophoretic mobility diagram.

Generally, electro-osmotic flow is the effect of the electrical double layer on the particle and T-shaped microchannel 112 surface. Therefore, by coating the surface of the T-shaped microchannel 112 with 1 wt% of bovine serum albumin (BSA), the velocity (and direction) of the particles can be measured by the pressure-driven flow and electric field Can be controlled by the electrophoretic force generated by the electrophoresis.

FIG. 3 is a view for explaining the measurement process of electroosmotic flow mobility.

Specifically, the microchannel was a linear type having a width of 200 mm, a height of 46 mm, and a length of 2 cm, and its surface was coated with 1 wt% of BSA. The microchannel was prepared by using PDMS (polydimethylsiloxane) on the substrate. A 0.1-mM DSP solution was applied to one side of the microchannel and a 1-mM DSP was applied to the other side of the microchannel After filling, a voltage of 100 V was applied to both ends of the microchannel, and the time (approximately 400 seconds) until the current reached a constant value was measured.

It can be seen that with 1, negligible compared to the electrophoretic mobility of the particles shown in Table 1 to the above-described electro-osmotic flow mobility measured on the basis of Figure 3 is 1 × 10 -4 ㎠V -1 s.

Meanwhile, FIG. 4 is a diagram showing the relationship between the applied electric field and the particle velocity of various sizes. The linear microchannel has a width of 200 μm, a height of 46 μm, a length of 2 cm, a particle flow rate of 299 nL min -1 , a particle diameter of 4.8 μm, an electrophoretic mobility of -4.35 × 10 -4 , A diameter of 9.9 mu m, an electrophoretic mobility of 6.18 x 10 < -4 >, a particle diameter of 10 mu m, and an electrophoretic mobility of -8.44 x 10 < -4 & gt ;.

As shown in FIG. 4, it can be seen that the flow velocity of all the particles (A, B, C) decreases linearly as the intensity of the electric field increases. In the electric field of a specific intensity, do. This means that the particles are moved in opposite directions, for example between about 4.5 and 7 (kV / m), assuming that two particles (A, B) with different electrophoretic mobility are introduced into the microchannel When an electric field is applied so as to have an intensity, it means that the particle A and the particle B move in the opposite direction, so that the particle A and the particle B can be separated.

FIG. 5 is a graph showing the electrophoretic mobility of the particles on the left side, the intensity of the electric field on the right side, and the intensity of the electric field on the lower side in FIG. 4 in relation to the particle size, electrophoretic mobility, Reference numeral 501 denotes a line connecting the electrophoretic mobility of each particle A, B and C, reference numeral 502 denotes a value obtained by connecting the intensity value of the critical electric field in which each particle A, B, It is.

As shown in FIGS. 4 and 5, it can be seen that the electric field of the particles is inversely proportional to the electrophoretic mobility of the particles and the intensity of the critical electric field in which each particle (A, B, C) changes directions.

6 is a diagram illustrating a process of separating micro-sized particles according to electrophoretic mobility in a specific critical electric field according to an embodiment of the present invention. The particles shown in Table 1 were used.

As can be seen in Figure 6 (a), Particle B (Particle B with an electrophoretic mobility value of -6.18) with a particle size of 9.9 占 퐉 has a particle size of 4.8 占 퐉 on the left (Particle A having an electrophoretic mobility value of -4.35) is moved to the right (in the pressure-driven flow direction) to separate particles A and B from each other. Further, as shown in FIG. 6 (b), the number of particles was measured, and the separation rate was 97% or more at one end and the other end of the microchannel.

Similarly, as can be seen from Fig. 6 (c), the particle C having a particle size of 10 mu m (particle C having an electrophoretic mobility value of -8.44) has a particle size of 9.9 mu m Particle B (Particle B with an electrophoretic mobility value of -6.18) is moved to the right (in the direction of pressure drive flow) to show that particles B and C are separated. Also, as shown in FIG. 7 (d), the number of particles was measured, and the separation rate was 97% or more at one end and the other end of the microchannel.

In Figure 6, T-shaped flow speed of the fluid flowing in the flow rate of the fluid (see Fig. 112a of 1) 400nLmin -1, and the channel sample flowing into the one end (114a) of the microchannel (see 112 in FIG. 1) is 100nLmin - 1 . In FIG. 6A, the applied voltage is 130V, and in FIG. 6C, the applied voltage is 80V.

Finally, FIG. 7 illustrates a process of separating nano-sized particles according to electrophoretic mobility according to an embodiment of the present invention.

The micro-sized particles are composed of a fluorescent dye molecule, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a, 4a-diaza-s-indacene-2,6-disulfonic acid , disodium salt (BODIPY 2 - Invitrogen) and 1,3,6,8 - pyrene tetrasulfonic acid (PTS 4 - Sigma - Aldrich) were used. Their electrophoretic mobility was PTS 4 - > BODIPY 2 - .

As shown in FIGS. 7A and 7B, BODIPY 2 - having a low electrophoretic mobility is moved to the right (in the direction of pressure driving flow) and PTS 4 - having a high electrophoretic mobility is moved to the left The reverse direction of the driving flow), it can be seen that the nano-sized particles are also well separated.

In Figure 7, T-shaped flow speed of the fluid flowing in the flow rate of the fluid (see Fig. 112a of 1) 300nLmin -1, and the channel sample flowing into the one end (114a) of the microchannel (see 112 in FIG. 1) is 30nLmin - 1 , and the applied voltage is 120V.

To observe and record the behavior of the particles described above, a CCD camera (Sensicam qe, Cook Corp.) and a fluorescence microscope (Axiovert 200, Zeiss) were used.

As described above, according to one embodiment of the present invention, an electric field is applied outside the T-shaped microchannel in the direction opposite to the direction of the pressure-driven flow to separate the particles according to the electrophoretic mobility, Therefore, the manufacturing process is complicated and difficult, and the problem of bubbles generated inside the device can be solved, and particles of various sizes can be separated continuously.

The present invention is not limited to the above-described embodiments and the accompanying drawings. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It will be self-evident.

110: chamber 111: substrate
112: T-shaped microchannel 112a: Sample channel
112b: separation channel 113: electrode part
113a: positive electrode 113b: negative electrode
114: first inlet passage 115: second inlet passage
116: Outflow channel 114a: One end of the separation channel (Outlet1)
114b: the other end of the separation channel (Outlet2)

Claims (10)

A sample channel into which a fluid containing charged particles flows, a separation channel through which a fluid for pressure-driven flow is passed and which is introduced from the sample channel and into which particles mixed with the fluid are separated, A chamber provided with a microchannel; And
And an electrode portion for applying an electric field outside the chamber,
Wherein the electrode part includes an anode provided at one end of both ends of the separation channel and a cathode provided at the other end of both ends of the separation channel,
When the electric field is applied, the particles are separated and moved to one end or the other end of the separation channel according to electrophoretic mobility of the particles,
Wherein particles having relatively large electrophoretic mobility move in a direction opposite to the direction of the pressure actuation flow and particles having relatively small electrophoretic mobility move in the direction of the pressure actuation flow, .
The method according to claim 1,
The electrode unit includes:
Wherein the particles are separated by adjusting the intensity of the electric field.
delete delete The method according to claim 1,
The surface of the T-
Particle separation device coated with 1wt% bovine serum albumin (BSA).
The method according to claim 1,
The particles,
A particle separator comprising particles having two sizes of electrophoretic mobility charged negatively.
The method according to claim 1,
The fluid may comprise,
A particle separation device using 1-mM DSP (Dibasic Sodium Phosphate) solution with a pH of 7.1 using pressure-driven flow and free-flow electrophoresis.
The method according to claim 1,
The particles,
A particle separation device comprising nanoscale or micro sized particles.
The method according to claim 1,
The particles,
BODIPY 2 - , PTS 4 - , particle separator containing polystyrene particles and glass particles.
The method according to claim 1,
The T-shaped microchannel is formed on a substrate,
The substrate may be made of glass, silicon, polymer plastic, pyrex, silicon dioxide, silicon nitride, quartz, polymethylmethacrylate (PMMA) A polycarbonate (PC), an acrylic or a cyclic olefin copolymer (COC).
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005087868A (en) * 2003-09-17 2005-04-07 Keio Gijuku Particle isolating process and apparatus therefor
KR101511569B1 (en) * 2013-11-14 2015-04-14 포항공과대학교 산학협력단 Particle separation apparatus

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005087868A (en) * 2003-09-17 2005-04-07 Keio Gijuku Particle isolating process and apparatus therefor
KR101511569B1 (en) * 2013-11-14 2015-04-14 포항공과대학교 산학협력단 Particle separation apparatus

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