KR101761769B1 - Color response measuring device for spectrometer and color response measuring method using thereof - Google Patents

Color response measuring device for spectrometer and color response measuring method using thereof Download PDF

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KR101761769B1
KR101761769B1 KR1020150135882A KR20150135882A KR101761769B1 KR 101761769 B1 KR101761769 B1 KR 101761769B1 KR 1020150135882 A KR1020150135882 A KR 1020150135882A KR 20150135882 A KR20150135882 A KR 20150135882A KR 101761769 B1 KR101761769 B1 KR 101761769B1
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color
spectrophotometer
color space
coordinates
blue
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KR20160036007A (en
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박민철
문성철
김재헌
우덕하
이동수
누엔 티엔 통 도
루이 다비드 르펄티에 티보
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한국과학기술연구원
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
    • G01J3/50—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
    • G01J3/52—Measurement of colour; Colour measuring devices, e.g. colorimeters using colour charts

Abstract

The present invention relates to an apparatus for measuring a color response of a spectrophotometer and a method of measuring a color response using the apparatus. The apparatus for measuring a color response of a spectrophotometer according to an embodiment of the present invention changes the structure by absorbing light, A measurement unit for inputting a target image into the spectrophotometer and measuring a first color coordinate for at least one reference color, and a controller for inputting the first color coordinate to a red cone cell, And a second color space formed by at least one of a conical cell and a blue conical cell.

Description

Technical Field [0001] The present invention relates to an apparatus for measuring color response of a spectrophotometer, and a color response measuring method using the same. ≪ Desc / Clms Page number 1 >

More particularly, the present invention relates to an apparatus for measuring a color response of a spectrophotometer based on a photoreceptor protein and a color response measuring method using the same.

Photoreceptor proteins are proteins that are present in the cell membrane of optic nerve cells and absorb light. The photoreceptor protein is made up of a combination of retinal, a compound produced by the oxidation of vitamin A, and opsin, a membrane protein. Opposite is a membrane protein present in the optic nerve cell membrane. Human has four types of opsin, idosin, which is present in cone cells and distinguishes the colors of red, green and blue, There is an optic to distinguish. The iodine and retinal combine to form photopsin, and the opsin and retinal combine to form rhodopsin. When the photoreceptor protein absorbs light, the optic and retinene are decomposed and energy is generated, and the energy is transferred to the cerebrum through the optic nerve to recognize the light.

On the other hand, a spectrophotometer is a device for measuring the intensity of light by wavelength, and the intensity of light is used for measuring the reflectance, transmittance and absorption rate of an object. The spectrophotometer is usually composed of a light source, a monochromator and a detector, and is configured to disperse the light into a wavelength component by a monochromator by a light source and measure the intensity of each wavelength component dispersed by using a detector.

However, according to the conventional spectrophotometer, it is difficult to simultaneously measure a wide-band light source such as a human eye. Although the light intensity of each wavelength can be measured by dividing the light into monochromatic light by a monochromator, it is difficult to perform a measurement of a complex light such as human vision.

It is also difficult to compare how the color measured by the spectrophotometer is the same as the color sensed by a normal person.

KR 10-2002-0011385 A

Expression, Solubilization and Purification of a Human Olfactory Receptor from Escherichia coli ", Curr. Microbiol. (2009) 59: 309-314 "Human Taste Receptor-Functionalized Field Effect Transistor as a Human-Like Nanobioelectronic Tongue", Nano Lett. (2013) 13: 172-178 Kim, Tae-Hyun et al., "Single-Carbon-Atomic-Resolution Detection of Odorant Molecules using a Human Olfactory Receptor-based Bioelectronic Nose ", Adv. Mater. (2009) 21: 91-94 Hyeon Seok et al., "Polypyrrole Nanotubes Conjugated with Human Olfactory Receptors: High-Performance Transducers for FET-Type Bioelectronic Noses", Angew. Chem. Int. Ed. (2009) 48: 2755-2758 Kim, Tae-Hyun, et al., "Bioelectronic super-taster" device based on taste receptor-carbon nanotube hybrid structures ", Lab Chip (2011) 11: 2262-2267

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a photoreceptor protein which is combined with a field effect transistor (FET) or the like to be a spectrophotometer capable of reproducing human vision, It is an object of the present invention to provide a color response measuring apparatus of a spectrophotometer which can analyze a color response of a spectrophotometer and make it possible to compare a detectable color space with a color space recognized by an actual human being and a color response measuring method using the apparatus .

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and an apparatus for measuring a color response of a spectrophotometer according to an embodiment of the present invention is characterized in that the structure is changed by absorbing light, A spectroscopic photometer comprising a photoreceptor protein comprising a cell, a green cone cell and a blue cone cell, a measurement unit for inputting the target image into the spectrophotometer and measuring a first color coordinate for one or more reference colors, And a conversion unit for projecting the color coordinates in a second color space which is an xyY color space composed of chromaticity and brightness.

delete

For example, the output value measured by the measuring unit is represented by first color coordinates (R, G, B) composed of red, green, and blue coordinates, and the first color coordinates are expressed by Can be projected into the X, Y, and Z color space, where the response by red cones, green cones and blue cones is the value of each axis.

(Equation 1)

Figure 112015093532473-pat00001
,
Figure 112015093532473-pat00002
The conversion coefficient

Next, when the projection value into the XYZ color space is

Figure 112016125704583-pat00003
,
Figure 112016125704583-pat00004
And
Figure 112016125704583-pat00005
(X, y, Y) of the second color space can be expressed by the following (formula 2).

(Equation 2)

Figure 112015093532473-pat00006
,
Figure 112015093532473-pat00007
,
Figure 112015093532473-pat00008

Wherein the spectrophotometer includes a field effect transistor including a plurality of sets each including a source electrode, a drain electrode, and a channel layer at least partially including a region between the source electrode and the drain electrode, And a photoreceptor protein located on the region between the photoreceptor proteins.

The target image may be composed of one of red, green, and blue, and may be composed of a plurality of images having different wavelengths.

In the method of measuring the color response of a spectrophotometer according to another embodiment of the present invention, the structure is changed by absorbing light to cause a change in electrical characteristics, and includes a stem cell, a red cone cell, a green cone cell, and a blue cone cell Measuring a first color coordinate with respect to at least one reference color by inputting a target image into a spectrophotometer including a photoreceptor protein including a photoreceptor protein having a first color coordinate and a second color space with an xyY color space composed of chromaticity and brightness, As shown in FIG.

delete

Wherein the first color coordinates are represented by color coordinates (R, G, B) composed of red, green, and blue coordinates, and the first color coordinates are represented by the following formula (1): red cones, The response by each cone cell can be projected into the XYZ color space with the value of each axis.

(Equation 1)

Figure 112015093532473-pat00009
,
Figure 112015093532473-pat00010
The conversion coefficient

The second color space is an xyY color space composed of chromaticity and brightness, and compares a color response by a spectrophotometer in a second color space and a color response by a predetermined visual cell. When the projection value into the XYZ color space is

Figure 112016125704583-pat00011
,
Figure 112016125704583-pat00012
And
Figure 112016125704583-pat00013
, The coordinates of the second color space may be expressed by the following equation (2).

(Equation 2)

Figure 112015093532473-pat00014
,
Figure 112015093532473-pat00015
,
Figure 112015093532473-pat00016

The target image may be composed of one of red, green, and blue, and may be composed of a plurality of images having different wavelengths.

According to an embodiment of the present invention, a color response detected using a spectrophotometer can be compared and analyzed with a color response by an actual visual cell.

Also, according to an embodiment of the present invention, a spectrophotometer can be accurately and precisely analyzed, and it is possible to compare a color response by an artificial retina or the like to which a spectrophotometer can be applied and a color response of an actual person.

FIG. 1 is a schematic view showing the shapes of opsin and retinal forming a photoreceptor protein.
2 is a graph showing the wavelength band of light absorbed by each of the four kinds of human photoreceptor proteins.
3 is a view schematically showing an apparatus for measuring a color response of a spectrophotometer according to an embodiment of the present invention.
4 is a schematic view of a spectrophotometer according to an embodiment of the present invention.
FIG. 5A is a graph showing the color response by the visual cell, and FIG. 5B is a chromaticity diagram showing the color response of FIG. 5A in the xyY color space.
6A is a graph showing various reference images of red, green and blue, FIG. 6B is a graph showing a color response of a spectrophotometer by reference images of FIG. 6A, FIG. 6C is a graph showing a color response of FIG. Fig.
FIG. 7A is a graph showing the Gaussian transformation with respect to the red wavelength in the color response of the spectrophotometer, and FIG. 7B is a chromaticity diagram showing the color response of FIG. 7A.
FIG. 8A is a graph showing the Gaussian transformation with respect to the green wavelength in the color response of the spectrophotometer, and FIG. 8B is a chromaticity diagram showing the color response of FIG. 8A.
FIG. 9A is a graph showing a Gaussian transformation and a noise region with respect to a blue wavelength in a color response of a spectrophotometer, and FIG. 9B is a chromaticity diagram showing a color response of FIG. 9A.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately The present invention should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and do not represent all the technical ideas of the present invention. Therefore, It is to be understood that equivalents and modifications are possible.

Embodiments of the present invention are directed to a color response measurement device and a color response measurement method using a photoreceptor protein-based spectrophotometer. Since the photoreceptor protein absorbs the applied light and its electrical characteristics change, a photoreceptor protein-based spectrophotometer can be realized.

According to an embodiment of the present invention, a protein-based spectrophotometer can be implemented in a form in which a photoreceptor protein and a field effect transistor are combined. It is possible to provide a color response measuring apparatus and a color response measuring method which can analyze the color response of a photoreceptor protein-based spectrophotometer compared with an actual human color perception by measuring the color response of the photoreceptor protein-based spectrophotometer.

FIG. 1 is a view schematically showing the shapes of opsin and retinal forming photoreceptor proteins used in a spectrophotometer.

The photoreceptor protein can function only when the opsin (FIG. 1 (a)) and the chromophore retinal (FIG. 1 (b)) are combined. Retinal is separated from the opsin as it changes in its form from 11- cis -retinal to all-trans-retinal upon receiving light. Obsin is a membrane protein belonging to G protein-coupled receptor (GPCR). As shown in the figure, its structure is complex and strong in its hydrophobic nature, so that it is difficult to express and produce it in xenogeneic cells.

However, a technique for producing olfactory receptors or taste receptors belonging to the same GPCR family in Escherichia coli is known and can be used to produce opsin from E. coli.

For example, a method for producing olfactory receptors or taste receptors in Escherichia coli is well known from Non-Patent Documents 1 to 5 described above. Specifically, the opsin gene may first be inserted into a carrier for expressing Escherichia coli to overexpress an opsin in E. coli. Next, the opsin can be produced by a method of purifying opsin from E. coli expressing ops. The produced opsin induces cell membrane remodeling with a commercial lipid membrane or induces refolding using a surfactant. Next, the opsonin induced by cell membrane reconstitution or refolding with a surfactant has a binding site where the retinal can selectively bind, so that the retinal can bind selectively thereto. Thus, by finally binding the retinal to the produced opsin, a photoreceptor protein for use in the examples can be obtained.

2 is a graph showing the wavelength band of light absorbed by each of the four kinds of human photoreceptor proteins.

2, the human photoreceptor protein comprises blue cones, rods, green cones, and red cones, and Fig. 2 is a graph showing that each of the cells (X-axis) and relative sensitivity (y-axis).

The four types of photoreceptor proteins absorb light of different wavelengths, allowing the human brain to distinguish light from light. A spectrophotometer according to an embodiment of the present invention can simulate human vision through one or more of four types of human photoreceptor proteins.

FIG. 3 is a view schematically showing an apparatus 1 for measuring a color response of a spectrophotometer according to an embodiment of the present invention.

3, a color response device 1 of a spectrophotometer according to an embodiment of the present invention includes a photoreceptor protein-based spectrophotometer 10, a measurement unit 20, and a conversion unit 30 .

The spectrophotometer 10 includes a photoreceptor protein as described above, which changes its structure by absorbing light, thereby causing a change in electrical characteristics. And, in the spectrophotometer 10, the photoreceptor protein can be implemented in a field effect transistor (FET), for example.

The measuring unit 20 inputs the target image to the spectrophotometer to measure a first color coordinate with respect to one or more reference colors, and the converting unit 30 converts the first color coordinate into a second color coordinate And can project in the color space.

In this specification, inputting an image to a spectrophotometer refers to irradiating the spectrophotometer with light corresponding to the image.

The present invention can measure and compare the color response by a spectrophotometer by projecting color coordinates measured by a spectrophotometer onto a color space that can be derived by visual cells.

More specifically, the first color coordinates of a target image are measured through a photoreceptor protein-based spectrophotometer 10. Then, the first color coordinates are projected in the second color space. The second color space corresponds to a color space that can be observed by a normal person by visual cells. By projecting the first color coordinate in the second color space, the color measured by the spectrophotometer can be observed by a normal person You can see what color is in the available color space.

Accordingly, when a spectrophotometer is applied to an artificial retina of a visually impaired person, it is possible to confirm which color corresponds to a color in a color space of a normal person, and to provide visual information similar or identical to a real human visual system It can be analyzed accurately.

FIG. 4 is a diagram specifically showing a photoreceptor protein-based spectrophotometer 10 according to an embodiment of the present invention.

The spectrophotometer 10 according to an embodiment of the present invention may be composed of a field effect transistor (FET) 100 and a photoreceptor protein 200.

In this embodiment, the photoreceptor protein 200 is embodied in the FET 100. However, the present invention is not limited thereto, and the electrical characteristics of the photoreceptor protein 200 whose structure is changed by absorbing light Various means of sensing and measuring can be applied thereto.

The FET 100 includes a substrate 110, a source electrode 131 mounted on the substrate 110, a channel layer 133, and a drain electrode 135.

The substrate 110 may be made of silicon or various materials known in the art and an insulating layer 120 may be disposed between the substrate 110 and the electrodes 131,

The source electrode 131 and the drain electrode 135 may be disposed at predetermined intervals and the channel layer 133 may be disposed at least partially between the source electrode 131 and the drain electrode 135 . The source electrode 131 and the drain electrode 135 may be made of metal or other conductive material.

The channel layer 133 is a layer for forming a channel through which a current flows between the source electrode 131 and the drain electrode 135, and may be formed of a semiconductor material, for example.

4, since the entire channel layer 133 is located between the source electrode 131 and the drain electrode 135, the entire region between the source electrode 131 and the drain electrode 135 described above is located between the channel layer 133 and the drain electrode 135, (133). It is needless to say that the channel layer 133 has a wider region and only a part of the channel layer 133 is positioned between the source electrode 131 and the drain electrode 135.

The photoreceptor protein 200 may be located on the surface of the FET 100. In one embodiment, the photoreceptor protein 200 may be located at least partially on the area between the source electrode 131 and the drain electrode 135 in the FET 100. 2, the photoreceptor protein 200 may include one or more of four kinds of proteins.

For example, when all four kinds of photoreceptor proteins 200 are bonded to the FET 100, since each photoreceptor protein reacts to light of different wavelengths, it absorbs light from the human eye and is recognized by the brain The process can be simulated by using the spectrophotometer 10. At this time, the FET 100 may include a source electrode 131, a drain electrode 135, and a channel layer 133 connected to the respective photoreceptor proteins 200. That is, the source electrode 131, the drain electrode 135, and the channel layer 133 are formed of a plurality of sets depending on the type of the photoreceptor protein 200, and each set includes a photoreceptor protein 200 of a different kind Can be connected.

The spectrophotometer 10 simulating human vision can be realized by measuring the change in the current generated in the FET 100 as the photoreceptor protein 200 absorbs light. For example, an electrode through which a current flows among the plurality of source electrodes 131 and the plurality of drain electrodes 135 is determined depending on the kind of the light-absorbing photoreceptor protein 200. The current flowing between the source electrode 131 and the drain electrode 135 is influenced by the intensity of light absorbed by the photoreceptor protein 200 and the like.

Further, referring to FIG. 2, different types of photoreceptor proteins 200 absorb light of different wavelengths. Therefore, by measuring the current in the measuring unit 20 and detecting the current from the electrode, the type of the photoreceptor protein 200 that absorbed the light, the wavelength of the absorbed light and / or the intensity of the absorbed light Can be calculated.

More specifically, when light is applied to the photoreceptor protein 200, the FET 100 functions to convert the light absorbed by the photoreceptor protein 200 into an electric signal. When the photoreceptor protein 200 absorbs light, the photoreceptor protein 200 undergoes a structural change such as separation of optic and retinal or conversion of photoreceptor protein structure.

This structural change causes the potential change of the photoreceptor protein 200 and the amount of current flowing between the source electrode 131 and the drain electrode 135 of the FET 100 due to the potential change of the photoreceptor protein 200 .

That is, the potential change by the photoreceptor protein 200 in the spectrophotometer 10 plays a role similar to the gate voltage in the FET 100, and changes the amount of current between the source electrode 131 and the drain electrode 135 .

Through the spectrophotometer 10 according to an embodiment of the present invention, the photoreceptor protein 200 can absorb the light from the human eye and simulate the brain recognition process. For example, when all of the above-described four types of photoreceptor proteins are used, the human eye can be reproduced using the principle that the human eye detects light.

The spectrophotometer 10 can simultaneously measure the wavelength and the intensity of light in a wide wavelength band, thereby overcoming the limitations of the conventional spectrophotometer.

In addition, the spectrophotometer 10 according to the embodiment of the present invention can reproduce the human vision, and thus can be utilized for artificial vision and the like. For example, the spectrophotometer 10 can be realized as an artificial retina.

The spectrophotometer 10 according to an embodiment of the present invention can be manufactured by utilizing various techniques known in the technical field of the present invention.

Although not limited thereto, the FET 100 may be formed by stacking and patterning an insulating material, a conductive material, a semiconductor material, or the like by various known methods. FET 100 may be a silicon-based FET or a nanomaterial-based FET, and is not limited to a particular structure and material.

After the FET 100 is fabricated, the photoreceptor protein-based spectrophotometer 10 can be fabricated by immobilizing the photoreceptor protein 200 on the FET 100. For example, the photoreceptor protein 200 may be prepared in advance by a method such as the expression of E. coli, but the present invention is not limited thereto.

The photoreceptor protein 200 can be immobilized on the surface of the FET 100 in a chemical and / or physical manner. The photoreceptor protein 200 is immobilized on the surface of the FET 100 such that it is at least partially located in the region between the source electrode 131 and the drain electrode 135 of the FET 100. In this embodiment, .

In order to immobilize the photoreceptor protein 200 on the surface of the FET 100, the FET 100 may be placed in the phosphate solution. Then, a functional group on the surface of the FET 100 can be formed through the surface treatment of the FET 100. [ The functional group is a substance capable of binding to the photoreceptor protein 200, and is not necessarily limited thereto, but a substance capable of peptide bonding with the photoreceptor protein 200 can be applied thereto.

Then, a photoreceptor protein 200 of about several milligrams (mg) is injected into the phosphate solution to bind the photoreceptor protein 200 to a functional group such as a carboxyl group or an amine group on the surface of the FET 100.

In the above embodiments, the photoreceptor protein-based spectrophotometer 10 has been described based on the implementation using the FET, but the present invention is not limited thereto. Any sensor capable of operating in a manner that detects light using the activation of photoreceptor proteins by light can be used as a photoreceptor protein-based spectrophotometer.

The color response measuring apparatus 1 of the spectrophotometer manufactured in the above manner comprises a measuring unit 20 for detecting a change in electrical characteristics from the spectrophotometer 10 to derive a first color coordinate, And a conversion unit (30) for conversion to project in the two color spaces.

The first color coordinates may be coordinates of at least one of red (R), green (G), and blue (B) as a coordinate of the first color space by one or more reference colors. According to one embodiment, the output value measured by the measuring unit 20 may be expressed by first color coordinates (R, G, B) composed of red, green, and blue coordinates.

Then, according to one embodiment, the first color coordinates (R, G, B) are converted into red, green, and blue cones (Coordinates (X, Y, Z)) corresponding to the values of the respective axes.

(Equation 1)

Figure 112015093532473-pat00017
,
Figure 112015093532473-pat00018
The conversion coefficient

The sRGB color space, the XYZ color space, and the xyY color space, which are described in accordance with an embodiment of the present invention, are defined by the International Lighting Association (CIE)

Figure 112015093532473-pat00019
May consist of a matrix of constants determined from the definition of each space. Specifically,
Figure 112015093532473-pat00020
Can be expressed as follows.

Figure 112015093532473-pat00021

The color can be expressed by chromaticity and brightness, and the color response by the spectrophotometer and the color response by the visual cell can be compared in the xyY color space composed of chromaticity (x, y) and brightness (Y) have. According to an embodiment of the present invention, the xyY color space may be a second color space.

According to an embodiment of the present invention, for projection into the xyY color space, the projection value into the XYZ color space is

Figure 112016125704583-pat00022
(Or "X"),
Figure 112016125704583-pat00023
(Or "Y") and
Figure 112016125704583-pat00024
(Or "Z"), the coordinates of the xyY color space can be expressed by the following equation (2).

(Equation 2)

Figure 112015093532473-pat00025
,
Figure 112015093532473-pat00026
,
Figure 112015093532473-pat00027

According to an embodiment of the present invention, the target image input to the spectrophotometer 10 may be composed of one of red, green, and blue, and may be composed of a plurality of images having different wavelengths.

In the case of a reference image composed of a plurality of images having different wavelengths, since the color response of the visual cell is already known, the color response derived by inputting into the spectrophotometer 10 can be easily .

Hereinafter, a color response measurement method using the color response device of the above-described spectrophotometer will be described in more detail. The configuration corresponding to the color response device can be equally applied to the color response measurement method.

A method of measuring a color response of a spectrophotometer according to an embodiment of the present invention includes inputting a target image to a spectrophotometer including a photoreceptor protein in which a structure is changed by absorbing light, Measuring a first color coordinate with respect to the reference color, and projecting the first color coordinate in a second color space, which is an xyY color space composed of chromaticity and brightness.

The target image is one of red, green, and blue, and is a reference image composed of a plurality of images having different wavelengths. The color response of the visual cell with respect to the reference image can be acquired from known data.

5A is a graph showing a color response by a human eye. 5B is a chromaticity diagram showing the result of FIG. 5A as a color space (xyY).

More specifically, the graph in FIG. 5A shows the color response of each of red cones, blue cones, and green cones by wavelength. The color response of visible light by these three kinds of cones can be expressed by three - dimensional information.

On the other hand, the color can be expressed in terms of brightness and chromaticity, and the above three-dimensional information can be projected in a space composed of dimensions of brightness and chromaticity.

Responses by red cones, green cones and blue cones, respectively

Figure 112015093532473-pat00028
,
Figure 112015093532473-pat00029
And
Figure 112015093532473-pat00030
, The color coordinates (x, y, Y) in the second color space are expressed by the following equation (1) when the result of FIG. 5A is projected onto the xyY color space defined by x, y and Y coordinate axes .

(Equation 1)

Figure 112015093532473-pat00031
,
Figure 112015093532473-pat00032
,
Figure 112015093532473-pat00033

X and y correspond to the chromaticity, and Y corresponds to the brightness. The color coordinates (x, y, Y) of the visual cell with respect to the reference image can be obtained through known data.

On the other hand, the color response by the visual cell appears in the chromaticity diagram of Fig. 5B, and corresponds to the color space recognized by the eye of the normal person as the region of the designated thimble shape. The color response of the spectrophotometer can be measured by comparing the color response area of the visual cell as shown in FIG. 5B with the color response of the spectrophotometer obtained through the following procedure.

Hereinafter, how the color measured through the photoreceptor protein-based spectrophotometer 10 is projected onto the color of the second color space will be described in more detail.

The target images are converted into light by irradiating the photoreceptor protein-based spectrophotometer 10, and the electrical change of the spectrophotometer 10 is measured by the measuring unit 20 in response thereto.

For example, in the case of a spectrophotometer based on a photoreceptor protein based on an FET type, the output value can be compared by measuring the intensity of current flowing between the source electrode and the drain electrode.

According to an embodiment of the present invention, the target image to be measured may be a reference image. More specifically, images that are used for measuring the color response of the photoreceptor protein-based spectrophotometer 10, the wavelength of which is known in advance, can be used. However, the present invention is not limited thereto.

A plurality of reference images are used for each wavelength, and an output value of the photoreceptor protein-based spectrophotometer 10 for each wavelength can be obtained.

6A are reference images according to various embodiments of the present invention. 6B is a graph showing an output value of the spectroscopic photometer 10 to the measurement part 20 with respect to one reference image of the reference images.

As shown in FIG. 6A, a plurality of images of red, green, and blue having different wavelengths are input as reference images, and the intensity of the output value for each wavelength is plotted as a graph Can be obtained.

The conversion unit 30 performs the following steps to project the output values obtained by the measurement unit 20 into the xyY color space, which is the second color space.

The sRGB color space coordinates (R, G, B) for reference images of red, green, and blue can be obtained based on the output value obtained by the measuring unit 20. [

The output value in sRGB coordinates can be projected in the XYZ color space to reflect the standard colorimetry. More specifically, the predetermined conversion coefficient

Figure 112015093532473-pat00034
(X, Y, Z) (X, Y, Z color space) by the following equation (2) by the following equation (2).

(Equation 2)

Figure 112015093532473-pat00035

Wherein the coordinates (X, Y, Z) are converted into second color coordinates (x, y, Y) in the second color space by the following (formula 3).

(Equation 3)

Figure 112015093532473-pat00036
,
Figure 112015093532473-pat00037
,
Figure 112015093532473-pat00038

6C is a chromaticity diagram showing a color response in which the output value of FIG. 6B is projected in the xyY color space by the above-described process. The area of the thimble shape designated by the chromaticity diagram of Fig. 6C corresponds to the color space recognized by the eyes of the normal person, and the area of the triangle A represents the color space detected by the spectrophotometer 10. Fig.

For example, when the spectrophotometer 10 is constructed as an artificial retina and is implanted, the triangle (A) region becomes a color space recognizable by the subject. The color space of the thimble-shaped visual cell can be compared with the color space of the spectrophotometer 10 of the triangle (A) region, and it can be confirmed which region can be recognized by the spectrophotometer 10. This allows the subject to easily see what color he or she is normally seeing and which color is hard to see.

The color response of the spectrophotometer 10 projected in the color space seen by the eyes of a normal person is affected by the detection characteristics of the spectrophotometer 10 with respect to the applied light. That is, the wavelength response of the light detected by the spectrophotometer 10, the peak position of the detected wavelength band, and the width of the line width centered on the peak position affect the color response.

According to an embodiment of the present invention, the influence of the spectrophotometer 10 on the color response according to the detection characteristics of the spectrophotometer 10 can be accurately compared and analyzed.

Fig. 7A is for explaining the change in the detection characteristic of the spectrophotometer, and Fig. 7B is for showing the color response in accordance with the change of the detection characteristic shown in Fig. 7A.

7A is a graph showing the results obtained by replacing the values corresponding to the red wavelengths in the output value of the spectrophotometer 10 with Gaussian distributions having standard deviations of 5, 6, 7, 8, and 10 with a wavelength of 610 nm as the center .

7B, the color response (B) of the spectrophotometer 10 indicates that as the line width of the peak corresponding to the red wavelength is narrowed as shown by the arrow b, the red region is narrowed Able to know.

8A is a graph for explaining changes in detection characteristics of a spectrophotometer, which is a graph in which output values of a green wavelength are changed, FIG. 8B is a graph showing changes in detection characteristics shown in FIG. 8A, to be.

8A is a graph showing the results obtained by replacing the output values of the spectrophotometer 10 with Gaussian distributions with standard deviations of 3, 5, 6, and 10, respectively, centering on a wavelength of 540 nm corresponding to green.

In this case, the color response C of the spectrophotometer 10 shows that the color space of the green region becomes narrower as the line width of the peak corresponding to the green wavelength is narrowed, as indicated by arrow c 1 and arrow c 2 have.

FIG. 9A is a graph for explaining a change in the detection characteristic of the spectrophotometer 10, and is a graph showing changes in output values of a blue wavelength. FIG. 9B is a chromaticity diagram showing a change in the color response as a change in the detection characteristic shown in FIG. 9A. FIG.

9A, when the values corresponding to the blue wavelengths in the output values of the photoreceptor protein-based spectrophotometer 10 are replaced with Gaussian distributions having standard deviations of 2 and 20 with a wavelength of 470 nm as the center, The color response C shows that the color space D that can be detected by the spectrophotometer 10 becomes narrow as the line width of the peak corresponding to the blue wavelength narrows as shown by the arrows d 1 and d 2 Able to know.

9A, noise (e) in the vicinity of the wavelength of 45 nm appears as noise (E) in the color space in FIG. 9B.

Photoreceptor proteins are activated by absorbing light, resulting in changes in electrical properties. Since a spectrophotometer based on a photoreceptor protein can analyze human vision using this, the absorption wavelength of each photoreceptor may be different from the absorption wavelength of a human specific receptor. Therefore, the photoreceptor protein-based spectroscopy The color of light detected using a photometer and the color of the actual human being may be different from each other.

However, according to one embodiment of the present invention, by detecting the color space measured by the photoreceptor protein-based spectrophotometer using the absorption wavelength band characteristic of the photoreceptor, and projecting it in a color space recognized by the human eye, A color range that can be detected with a protein-based spectrophotometer can be confirmed. When the photoreceptor protein-based spectrophotometer is implemented in the form of an artificial retina for the visually impaired, the range of colors that the visually impaired person can see through the color response measuring apparatus and the color response measuring apparatus of the present invention is compared with that of a normal person .

While the invention has been shown and described with reference to certain embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. However, it should be understood that such modifications are within the technical scope of the present invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

1: Color response measurement device of spectrophotometer
10: Spectrophotometer
20:
30: conversion section
100: field effect transistor
110: substrate
120: insulating layer
131: source electrode
133: channel layer
135: drain electrode
200: photoreceptor protein

Claims (11)

A spectrophotometer comprising a photoreceptor protein including a photoreceptor protein including a stem cell, a red cone cell, a green cone cell, and a blue cone cell, which is changed in structure due to the absorption of light, resulting in a change in electrical characteristics;
A measuring unit for inputting a target image into the spectrophotometer and measuring a first color coordinate for at least one reference color; And
And a conversion unit for projecting the first color coordinates into a second color space which is an xyY color space composed of chromaticity and brightness,
The output value measured by the measuring unit is represented by first color coordinates (R, G, B) composed of red, green, and blue coordinates,
Wherein,
The first color coordinates are projected in an XYZ color space in which the response by each of the red cones, the green cones and the blue cones is the value of each axis,
(Equation 1)
Figure 112016125704583-pat00070
,
Figure 112016125704583-pat00071
The conversion coefficient
Projecting the projected values into the XYZ color space in the second color space, and projecting the coordinates in the XYZ color space into (
Figure 112016125704583-pat00072
,
Figure 112016125704583-pat00073
,
Figure 112016125704583-pat00074
), The coordinates (x, y, Y) of the second color space are expressed by the following equation (2).
(Equation 2)
Figure 112016125704583-pat00075
,
Figure 112016125704583-pat00076
,
Figure 112016125704583-pat00077

 delete delete delete The method according to claim 1,
Wherein the spectrophotometer comprises:
Further comprising: a field effect transistor including a plurality of sets each including a source electrode, a drain electrode, and a channel layer at least partially including an area between the source electrode and the drain electrode,
Wherein the photoreceptor protein is located on a region between the source electrode and the drain electrode of the field effect transistor and a plurality of different photoreceptor proteins are located in the plurality of sets.
The method according to claim 1,
Wherein the target image is composed of one of red, green, and blue, and is composed of a plurality of images having different wavelengths.
A method of measuring a color response of a spectrophotometer,
By changing the structure by absorbing light, a change in electrical characteristics is caused, and a target image is input to a spectrophotometer containing a photoreceptor protein including a light cell, a red cone cell, a green cone cell, and a blue cone cell, Measuring a first color coordinate with respect to the reference color;
Projecting the first color coordinate in a second color space which is an xyY color space composed of chromaticity and brightness; And
And comparing the color response by the spectrophotometer projected in the second color space with a predetermined color response by the visual cell,
Wherein the first color coordinates are represented by color coordinates (R, G, B) composed of red, green, and blue coordinates,
Wherein the projecting in the second color space comprises:
Projecting the first color coordinates into an XYZ color space having a response of each of the red cones, the green cones and the blue cones according to (Equation 1); And
(Equation 1)
Figure 112016125704583-pat00078
,
Figure 112016125704583-pat00079
The conversion coefficient
The coordinates projected in the XYZ color space are (
Figure 112016125704583-pat00080
,
Figure 112016125704583-pat00081
,
Figure 112016125704583-pat00082
(X, y, Y) of the second color space using the following equation (2): " (1) "
(Equation 2)
Figure 112016125704583-pat00083
,
Figure 112016125704583-pat00084
,
Figure 112016125704583-pat00085

 delete delete delete 8. The method of claim 7,
Wherein the target image comprises one of red, green, and blue, and is comprised of a plurality of images having different wavelengths.
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