KR101760685B1 - Health functional food for preventing stomach cancer - Google Patents

Abstract

The present invention relates to a health functional food for preventing or ameliorating a tumor, and more particularly, to a health functional food which is free of toxicity including extracts derived from natural or natural substances and has high antitumor activity and is suitable for tumor prevention or improvement.

Description

{Health functional food for preventing stomach cancer}

The present invention relates to a health functional food for preventing or ameliorating a tumor, and more particularly, to a health functional food having a high antitumor activity without any toxicity and suitable for tumor prevention or improvement. .

Cancer refers to a malignant tumor that is abnormally developed in the cell cycle of a cell that normally constitutes a human tissue, and does not normally differentiate and grow so that growth can not be controlled. Cancer occurs through three stages of initiation, promotion, and progression. It can occur with only a sufficient amount of carcinogens, but in practice, a small amount of initiator It is known that cancer cells begin to form when abnormal cell division occurs due to stimulation of tumor promotor and the cell number increases exponentially as a result of cell mutation (initiation cell).

The global cancer incidence is increasing by more than 5% annually due to environmental deterioration and the increase of old age population. In 1997, 6 million deaths from cancer caused 12% of disease deaths. In Korea, 100,000 cancer patients develop annually, 50,000 deaths annually, and the rate of cancer patients growth reaches 10%. According to statistics of the National Cancer Center in 1999, the number of patients was 120,000, and men had gastric cancer (24%), liver cancer (16%), lung cancer (16%) and colon cancer ), Cervical cancer (12%), breast cancer (15%) and colorectal cancer (10%).

Surgical treatment, chemotherapy, biotherapy, radiation therapy, etc. have been used for the treatment of such cancers. Chemotherapy is a commonly used drug therapy that uses synthetic chemicals such as nucleic acid alkylating agents, metabolic antagonists, natural products, and hormones. Biotherapy restores or enhances the immune function of the body, weakening the activity of cancer cells Cytokine, immunotherapeutic agent, gene therapy agent, cancer vaccine, neovascularization inhibitor and the like. To date, these methods have been used either alone or in combination for treatment, but there is no complete treatment due to side effects, drug resistance, or recurrence.

So far, anticancer drug research has been carried out on the basis of cytotoxicity of cancerous cells, like cancer cells, and they are toxic to normal tissue cells such as skin, mucous membrane and bone marrow, which are rapidly dividing cells. Therefore, in order to develop an effective anticancer agent, it is necessary to search for an anticancer active substance which acts specifically on cancer cells and which is less toxic to normal cells. Currently, many researches on anticancer substances extracted from plants and microorganisms have been carried out, but most of them are mainly secondary metabolites of microorganisms or studies on the substances extracted from herbal medicines.

However, in order to solve the toxicity problem of anticancer substances, the stability is very high if we use food crops which are commonly consumed in everyday life and proven to be stable, rather than microorganisms or herbaceous plants.

(1-6) -glucanase [? - (1-6) -glucanase] in the case of the conventional mushroom-derived beta-glucan is disclosed in Patent Document 10-1345734 (Published on May 03, 2013) As an active ingredient, a modified beta-glucan having an intracellular content of 0.50 to 0.24 obtained by hydrolysis of a compound of the present invention as an active ingredient, and a pharmaceutical composition for the treatment of cancer diseases.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a health functional food having toxicity and high antitumor activity including extracts derived from natural or natural products.

The present invention provides a health functional food for preventing or ameliorating tumors, including Yumu and Agaricus mushroom.

In one preferred embodiment of the present invention, the yulmu comprises at least one of the group consisting of yulmu powder, yulmu concentrate and yulmu extract, and the agaricus mushroom is at least one kind selected from the group consisting of an agaricus mushroom fraction and an agaricus mushroom extract . ≪ / RTI >

In a preferred embodiment of the present invention, the health functional food may further include at least two of ginger, dandelion, pine needle, and glycyrrhizin.

In one preferred embodiment of the present invention, the health functional food may include 10-100 parts by weight of agaricus mushroom, 70-150 parts by weight of dandelion, and 30-90 parts by weight of ginger, based on 100 parts by weight of yulmu.

In one preferred embodiment of the present invention, the health functional food may further include 10 to 60 parts by weight of glycyrrhizin per 100 parts by weight of yulmu.

In one preferred embodiment of the present invention, the health functional food may further include 50 to 200 parts by weight of pine needles per 100 parts by weight of yulmu.

In a preferred embodiment of the present invention, the health functional food may include 80-100 parts by weight of agaricus mushroom, 100-150 parts by weight of dandelion, and 60-90 parts by weight of ginger, based on 100 parts by weight of yulmu.

In one preferred embodiment of the present invention, the agaricus mushroom, dandelion and ginger may be contained in a weight ratio of 1: 1 to 1.5: 0.6 to 0.9.

In one preferred embodiment of the present invention, the health functional food may include 80-100 parts by weight of agaricus mushroom, 100-150 parts by weight of dandelion, and 40-60 parts by weight of glycyrrhizin, based on 100 parts by weight of yulmu.

In a preferred embodiment of the present invention, the tumor may comprise at least one of the group consisting of pancreatic cancer, stomach cancer, duodenal cancer and small intestine cancer.

The present invention provides a health functional food which is free from toxicity and has high antitumor activity, including extracts derived from natural products or natural products, and is suitable for the prevention of gastric cancer.

Figs. 1 to 5 show the results of measuring the cancer cell proliferation inhibitory ability in Experimental Example 1. Fig.

Hereinafter, the present invention will be described in more detail.

As described above, most of conventional cancer treatment methods such as surgical treatment, chemotherapy, biotherapy, and radiation therapy are applied to treatment by using alone or in combination, but there is a problem that there is no perfect treatment method due to side effects, drug resistance, recurrence, and the like.

Accordingly, the present invention sought to solve the above-mentioned problems by providing a health functional food for preventing or ameliorating tumors, including Yumu and Agaricus mushroom. Accordingly, there is an effect of providing a health functional food which is less toxic than a conventional anticancer therapeutic agent and contains natural or natural-derived extracts, has high antitumor activity and is suitable for tumor prevention or improvement.

The Coix lachryma - jobi there is . ma - yuen ) is not particularly limited as long as it can be harvested and / or purchased normally, and the form thereof is not particularly limited, but may include one or more of the group consisting of yulmu powder, yulmu fractions and yulmu extract have.

The yulmu powder is not particularly limited as long as it can be generally harvested and / or purchased. For example, powders obtained by pulverizing and / or crushing the fruit of Yulmu can be used.

The yulmu fraction is not particularly limited as long as it is a conventional yulmu fraction that can be obtained using yulmu. Preferably, the yulmu fraction may be one prepared by extracting yulmu with an organic solvent 1 and then fractionating with an organic solvent 2.

The yulmu extract is not particularly limited as long as it is a conventional yulmu extract that can be obtained by using yulmu, but may be one prepared by extracting yulmu with an organic solvent 1.

The organic solvent 1 is not particularly limited as long as it is ordinarily used for extracting an extract from a natural product. Preferably, the organic solvent 1 is selected from the group consisting of water, C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, hexane and acetic acid And more preferably at least one kind selected from the group consisting of water, C ₁ -C ₄ alcohol, chloroform, hexane and dichloromethane.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The organic solvent 2 is not particularly limited as long as it is usually used for extracting an extract from a natural product. Preferably, the organic solvent 2 is extracted with hexane, a C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, methylene chloride, ethyl acetate, And more preferably at least one kind selected from the group consisting of chloroform, methylene chloride, ethyl acetate, hexane, butanol, and methanol.

The fraction is not particularly limited as long as it is usually used for fractionation of a fraction from a natural product. For example, chromatography, solvent fractionation or electrophoresis can be performed.

In one preferred embodiment of the present invention, the yulmu comprises at least one of the group consisting of yulmu powder, yulmu concentrate and yulmu extract, and the agaricus mushroom is at least one kind selected from the group consisting of an agaricus mushroom fraction and an agaricus mushroom extract . ≪ / RTI >

The agaricus mushroom ( Agaricus blazei Murill is not particularly limited as long as it can be conventionally prepared and / or purchased, and its form is not particularly limited, but may include at least one of the group consisting of agaricus mushroom powder and agaricus mushroom extract.

The above-mentioned agaricus mushroom powder is not particularly limited as long as it can be usually prepared and / or purchased. For example, a powder obtained by pulverizing and / or disrupting agaricus mushroom can be used.

The above-mentioned agaricus mushroom extract is not particularly limited as long as it is an agaricus mushroom extract which can be usually prepared and / or purchased, but it may be one prepared by extracting a mixture containing agaricus mushroom and an extraction solvent.

The extraction solvent is not particularly limited as long as it is usually used for extracting an extract from a natural product, but it is preferably selected from the group consisting of water, a C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, methylene chloride and acetic acid And more preferably at least one kind selected from the group consisting of water, methanol, ethanol, chloroform and dichloromethane.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The health functional food may contain 10 to 100 parts by weight of agaricus mushroom per 100 parts by weight of agaricus, preferably 100 to 100 parts by weight of agaricus mushroom, 50 to 100 parts by weight, and more preferably 80 to 100 parts by weight.

If 100 parts by weight of agaricus is contained in an amount less than 10 parts by weight of agaricus mushroom, antitumor activity is low and it may be difficult to use it as a health functional food. If 100 parts by weight of agaricus mushroom The antitumor activity is low compared to the added agaricus mushroom, which may result in an economical problem.

According to an embodiment of the present invention, the health functional food may further include at least two of the group consisting of ginger, dandelion, pine needle, and glycyrrhizin.

The ginger (Zingiber officinale is not particularly limited as long as it can be conventionally prepared and / or purchased, but it may preferably include at least one kind selected from the group consisting of ginger powder, ginger extract and ginger fraction.

The ginger extract is not particularly limited as long as it can be usually prepared and / or purchased. Preferably, the ginger extract may be obtained by adding organic solvent 3 to at least one part of the group consisting of ginger leaf, stem and root.

The organic solvent 3 is not particularly limited as long as it is generally used for extracting an extract from a natural product. Preferably, the organic solvent 3 is selected from the group consisting of water, C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, methylene chloride and acetic acid , And more preferably at least one kind selected from the group consisting of water, ethanol, butanol, chloroform and dichloromethane.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The ginger fraction is not particularly limited as long as it is a ginger fraction that can be obtained by a conventional fractionation method. Preferably, the ginger fraction may be prepared by extracting ginger with an organic solvent 3 and then fractionating it with an organic solvent 4.

The organic solvent 4 is not particularly limited as long as it is usually used for fractionating a fraction from a natural product. Preferably, the organic solvent 4 is a mixture of hexane, C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, methylene chloride, ethyl acetate, And more preferably at least one kind selected from the group consisting of chloroform, methylene chloride, ethyl acetate, hexane, and methanol.

The fraction is not particularly limited as long as it is usually used for fractionation of a fraction from a natural product. For example, chromatography, solvent fractionation or electrophoresis can be performed.

Furthermore, the health functional food may be contained in an amount of 30 to 90 parts by weight, preferably 60 to 90 parts by weight, based on 100 parts by weight of iridium.

If ginger is contained in an amount of 30 parts by weight per 100 parts by weight of ginseng, there may be a problem of low antitumor activity which may be caused by the addition of ginseng, and more than 90 parts by weight of ginseng per 100 parts by weight of ginseng , The antitumor activity is lower than that of the added ginger, resulting in an economical problem.

The dandelion ( Taraxacum platycarpum Dahlst . ) Is not particularly limited as long as it can be usually prepared and / or purchased, but it may preferably include at least one of the group consisting of a dandelion powder, a dandelion extract and a dandelion fraction.

The above-mentioned Dandelion extract is not particularly limited as long as it can be produced and / or purchased in a usual manner, but it may be obtained by adding an organic solvent 5 to at least one part of the group consisting of flowers, leaves, stems and roots of dandelion .

The organic solvent 5 is not particularly limited as long as it is generally used for extracting an extract from a natural product. Preferably, the organic solvent 5 is at least one selected from the group consisting of water, a C ₁ -C ₄ alcohol, acetonitrile, methylene chloride and acetic acid And more preferably at least one of the group consisting of water and a C ₁ to C ₄ alcohol.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The dandelion fraction is not particularly limited as long as it is a dandelion fraction that can be obtained by a conventional fractionation method. Preferably, the dandelion fraction may be prepared by extracting dandelion with an organic solvent 5 and then fractionating it with an organic solvent 6.

The organic solvent 6 is not particularly limited as long as it is generally used for fractionating fractions from natural products, but is preferably selected from the group consisting of hexane, C ₁ -C ₄ alcohol, chloroform, dichloromethane, acetonitrile, methylene chloride, ethyl acetate, And more preferably one or more of the group consisting of water, methylene chloride, ethyl acetate, hexane, and ethanol.

The fraction is not particularly limited as long as it is usually used for fractionation of a fraction from a natural product. For example, chromatography, solvent fractionation or electrophoresis can be performed.

In addition, the health functional food may contain 70 to 150 parts by weight of dandelion, preferably 100 to 150 parts by weight, based on 100 parts by weight of iridium.

If the dandelion is contained in an amount of 70 parts by weight based on 100 parts by weight of the yulmu, the antitumor activity which may be caused by the addition of the dandelion may be low. If the dandelion exceeds 150 parts by weight per 100 parts by weight of yulmu , The antitumor activity is lower than that of the added dandelion, which is not economical.

The pine needle refers to the leaf part of the pine, which is an evergreen evergreen tree, and is not particularly limited as long as it can be usually obtained and / or purchased, but preferably includes at least one of the group consisting of pine leaf extract, pine needles fraction and pine needle powder can do.

The pine leaf extract is not particularly limited as long as it can be prepared and / or purchased normally, but may be extracted by adding an organic solvent 7 to pine needles.

The organic solvent 7 is not particularly limited as long as it is generally used for extracting an extract from a natural product, but is preferably water, a C ₁ -C ₄ alcohol, acetonitrile, methylene chloride, chloroform, 1,3-butylene glycol And ethyl acetate, and more preferably at least one kind selected from the group consisting of water, methanol, ethanol, ethyl acetate and chloroform.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The pine needles fraction is not particularly limited as long as it is a pine needles fraction that can be obtained by a usual fractionation method. Preferably, the pine needles may be prepared by extracting the pine needle extract with an organic solvent 7 and then fractionating with an organic solvent 8.

The organic solvent 8 is not particularly limited as long as it is generally used for fractionating fractions from natural products, but is preferably selected from the group consisting of C ₁ -C ₄ alcohols, chloroform, dichloromethane, acetonitrile, methylene chloride, ethyl acetate and water And more preferably one or more of water, methylene chloride, ethyl acetate, hexane, butanol, ethanol, and methanol.

The fraction is not particularly limited as long as it is usually used for fractionation of a fraction from a natural product. For example, chromatography, solvent fractionation or electrophoresis can be performed.

In addition, the health functional food may be contained in 50 to 200 parts by weight, preferably 150 to 200 parts by weight, of pine needle in relation to 100 parts by weight of yulmu.

If the pine needle is contained in an amount of less than 50 parts by weight with respect to 100 parts by weight of the yulmu, there may arise a problem that the antitumor activity which can be caused by the addition of the pine needle is low, When pine needle is included, antitumor activity is lower than the amount of pine needle added, which may cause a problem that it is not economical.

Glycyrrhizin represented by the following formula (1) is taken from roots of licorice root and has a sweetness of 30 to 50 times that of sugar, and is used as an artificial sweetener or the like, and is not particularly limited as long as it can be manufactured and / , Preferably root-derived extract of licorice root, or glycyrrhin powder.

[Chemical Formula 1]

The glycyrrhizin is not particularly limited as long as it can be usually prepared and / or purchased, but it may be contained in the licorice extract which is extracted by adding the organic solvent 9 to the root portion of licorice.

The organic solvent 9 is not particularly limited as long as it is generally used for extracting an extract from a natural product. Preferably, the organic solvent 9 is at least one selected from the group consisting of water, C ₁ -C ₄ alcohol, acetonitrile, chloroform and acetone And more preferably at least one kind selected from the group consisting of water, ethanol, chloroform and acetone.

The extraction is not particularly limited as long as it is generally used for extracting an extract from a natural product. Supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction or ultrasonic extraction may be used, High pressure extraction or ultrasonic extraction may be used.

The health functional food may include 10 to 60 parts by weight of glycyrrhizine, preferably 40 to 60 parts by weight, based on 100 parts by weight of yulmu.

If 10 parts by weight of glycyrrhizin is contained per 100 parts by weight of the yulmu, the antitumor activity which may be caused by the addition of glycyrrhizin may be low, and more than 60 parts by weight of glycyrrhizin may be added to 100 parts by weight of yulmu , The antitumor activity is lower than that of the added glycyrrhizin, which may result in an economical problem.

In one preferred embodiment of the present invention, the health functional food may include 10-100 parts by weight of agaricus mushroom, 70-150 parts by weight of dandelion, and 30-90 parts by weight of ginger, based on 100 parts by weight of iridium. When the above-mentioned range of the above-mentioned agaricus mushroom, dandelion and ginger is included in the above range, it can exhibit excellent anti-tumor activity.

In one preferred embodiment of the present invention, the health functional food may contain 80-100 parts by weight of agaricus mushroom, 100-150 parts by weight of dandelion, and 60-90 parts by weight of ginger, relative to 100 parts by weight of yulmu, It is preferable that 90 to 100 parts by weight of agaricus mushroom, 120 to 150 parts by weight of dandelion and 80 to 90 parts by weight of ginger are contained in 100 parts by weight of yulmu.

At this time, the agaricus mushroom, dandelion and ginger may be contained in a weight ratio of 1: 1 to 1.5: 0.6 to 0.9, more preferably 1: 1.2 to 1.3: 0.8 to 0.9. When the above-mentioned range of the above-mentioned agaricus mushroom, dandelion and ginger is included in the above range, it can exhibit excellent anti-tumor activity.

In one preferred embodiment of the present invention, the health functional food may include 80-100 parts by weight of agaricus mushroom, 100-150 parts by weight of dandelion and 40-60 parts by weight of glycyrrhizin, It is preferable that 90 to 100 parts by weight of agaricus mushroom, 120 to 150 parts by weight of dandelion and 40 to 60 parts by weight of glycyrrhizin are contained. The health functional food produced in the above composition range exhibits excellent antioxidant activity.

According to another embodiment of the present invention, the tumor may comprise at least one of the group consisting of pancreatic cancer, stomach cancer, duodenal cancer and small intestine cancer.

The kind of health functional food is not particularly limited as long as it is usually produced and / or sold. For example, dairy products such as meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums and ice cream, soups, drinks, tea, drinks, alcoholic beverages, And can be used in the form of pills, powders, granules, infusions, tablets, capsules or beverages, all of which include health functional foods in the conventional sense.

The health beverage composition of the present invention is not particularly limited to a liquid ingredient except that it contains the above-mentioned anti-inflammatory active composition and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages.

Usually, the amount of the anti-inflammatory active composition contained in the health functional food composition may include 0.1 to 50% by weight, preferably 1 to 40% by weight of the total food. In addition, in the case of long-term ingestion intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

Hereinafter, the present invention will be described in more detail with reference to the following Examples. However, these drawings and the following embodiments are only illustrative of the contents and scope of the technical idea of the present invention, and the technical scope of the present invention is not limited or changed. Also, various modifications and alterations within the scope of the technical idea of the present invention based on these examples can be easily determined by those skilled in the art.

[ Example ]

Preparation Example  1. Yulmu Powder

The yulmu used in the present invention was purchased from Korean market at Yeongcheon market in Yeoncheon-gun, Gyeonggi-do, washed, lyophilized and ground to prepare 1 kg of yulmu powder.

Preparation Example  2. Ethyl alcohol extract

1 kg of the yulmu powder obtained in Preparation Example 1 and ethanol were mixed at a ratio of 1: 5 by volume and then extracted three times at 25 ° C to prepare an extract. The extract was filtered on a filter paper and concentrated under reduced pressure to obtain 200 g of yuumu ethanol extract .

Preparation Example  3. Yulmu Water extract

100 g of yulmu water extract was obtained in the same manner as in Preparation Example 2, except that water was used as the extraction solvent and Yulmu powder and water were mixed at a ratio of 1:10 by volume and extracted at 80 캜.

Preparation Example  4. Yulmu fraction

200 g of ethyl alcohol extract was obtained in the same manner as in Preparation Example 2 except that methanol was used as the extraction solvent. Then, 200 g of ethyl alcohol extract was suspended in 2 L of distilled water, and the mixture was partitioned with hexane, dichloromethane, ethyl acetate and butanol. The fractions were concentrated using a fractional rotary vacuum concentrator, and then fractionated with hexane, dichloromethane, ethyl acetate and butanol Fractions were obtained.

Preparation Example  5. Agaricus mushroom ethanol extract

Agaricus mushroom was dried and purchased from medicinal mushroom farming association and powdered using a pulverizer (WM-500, Unam Co., Yangsan, Korea) and passed through a standard mesh (40 mesh). Agaricus mushroom powder (1 kg) was mixed with 5 L of 70% ethanol and stirred at 25 ° C for 8 hours. The mixture was extracted three times to prepare an extract. The extract was filtered through a filter paper and concentrated under reduced pressure to obtain 350 g of an agaricus mushroom ethanol extract Respectively.

Preparation Example  6. Agaricus mushroom Water extract

200 g of Agaricus mushroom hot water extract was obtained in the same manner as in Preparation Example 2 except that water was used as the extraction solvent and the agaricus mushroom powder and water were mixed at a ratio of 1:20 by volume and then the mixture was heat-extracted at 95 ° C for 1 hour .

Preparation Example  7. Pine needles Water extract

The pine leaves used in the present invention were obtained by pulverizing and drying powdered leaves harvested from the mountains of Yeoncheon, Gyeonggi-do for 3 days, and then pulverizing them into 100 mesh sieves to prepare 500 g of pine leaf powder. Then, 100 g of the pine needle dried powder and water were mixed at a volume ratio of 1: 30, and the mixture was stirred at 100 ° C for 6 hours for thermal extraction, filtered through a filter paper and concentrated under reduced pressure to obtain 150 g of pine needle extract.

Preparation Example  8. Pine needles Fraction

80% ethanol was used as an extraction solvent, and 100 g of pine needle powder and 2 L of ethanol were mixed. The mixture was refluxed for 4 hours and lyophilized to obtain a pine needle methanol extract. Thereafter, the pine needle ethanol extract was suspended in 2 L of distilled water and fractionated with hexane, chloroform, ethyl acetate and butanol. The fractions were concentrated using a fractional rotary vacuum concentrator to obtain hexane fractions, chloroform fractions, ethyl acetate fractions and butanol fractions .

Preparation Example  9. Dandelion Water extract

The dandelion used in the present invention ( Taraxacum platycarpum Dahlst . ) Was prepared by drying only the dandelion leaves, stems and roots after sterilization of green dandelion flowers grown in environmentally friendly Buonnam-myeon, Gyeongsangbuk-do, and sterilized at 121 ℃ for 30 minutes. 1 kg of dandelion powder was prepared. Then, 500 g of the dandelion powder and water were mixed at a volume ratio of 1:20, and the mixture was stirred for 3 hours at 100 ° C for one hour. The mixture was filtered three times and filtered. The filtrate was concentrated under reduced pressure to obtain 180 g of a water extract of dandelion.

Preparation Example  10. Dandelion Fraction

200 g of ethanol extract of dandelion was obtained in the same manner as in Preparation Example 9, except that ethanol was used as the extraction solvent. Then, 200 g of the ethanol extract of dandelion was suspended in 2 L of distilled water, and the fractions were fractionated with water, hexane, methylene chloride, ethyl acetate and ethanol, and concentrated using a fractional rotary vacuum concentrator. The water fraction, hexane fraction, methylene chloride fraction , Ethylacetate fractions and ethanol fractions were obtained.

Preparation Example  11. Glycyrrhizin  extract

Glycyrrhizin used in the present invention was cultivated at a planting density of 50 cm * 20 cm at the planting area of the medicinal crop test package of the crop field, and was washed with water after being harvested and dried in a dryer at 60 ° C to obtain a root diameter of 0.7 cm. Specifically, 250 mg of distilled water was added to 20 g of the above-mentioned licorice by-product, and the mixture was stirred at room temperature for 5 hours while being repeatedly extracted three times.

Preparation Example  12. Ginger Powder

The ginger used in the present invention was purchased from Gapwal Food Co., Ltd. (Korea) and pulverized with a blender (SFM-2800B, Shinil Industrial Co., Ltd., Seoul, Korea) 1 Kg of powder was used.

Preparation Example  13. Ginger Water extract

The ginger powder obtained in Preparation Example 12 and water were mixed at a volume ratio of 1:10, and the mixture was stirred at 90 ° C for 5 hours for extraction. The extract was extracted three times in total, and the extract was filtered on a filter paper And concentrated under reduced pressure to obtain 360 g of ginger hot-water extract.

Example  1. Manufacture of Health Functional Foods

A health functional food was prepared by mixing 100 g of the yulmu powder of Preparation Example 1 and 50 g of the ethanolic extract of the agaricus mushroom of Preparation Example 5.

Example  2 ~ 15. Manufacture of Health Functional Food

Were mixed at the mixing ratios shown in Table 1 below to prepare health functional foods.

Ingredients and content Example 2 Preparation Example 1 100 g of powder of yulmu Preparation Example 5 Ethanol extract of Agaricus mushroom 80 g Example 3 10 g of yu-mu ethanol extract of Preparation Example 2 Preparation Example 5 Agaricus mushroom ethanol extract 5 g Preparation Example 7 Pine needles water extract 5g Example 4 10 g of yu-mu ethanol extract of Preparation Example 2 Preparation Example 5 Agaricus mushroom ethanol extract 5 g Preparation Example 8 Pine needle leaf hexane fraction 5 g Example 5 10 g of yu-mu ethanol extract of Preparation Example 2 Preparation Example 6 Agaricus mushroom water extract 5 g Preparation Example 8 Pine needle leaf hexane fraction 15 g Example 6 10 g of Yulmu water extract of Preparation Example 3 Preparation Example 5 Agaricus mushroom ethanol extract 8 g Preparation Example 9 Dandelion seed extract 7 g Example 7 10 g of Yulmu water extract of Preparation Example 3 Preparation Example 6 Agaricus mushroom water extract 8 g Preparation Example 9 Dandelion water extract 13 g Example 8 10 g of the urumuhexane fraction of Preparation Example 4 Preparation Example 6 Agaricus mushroom water extract 8 g Preparation Example 10 Dandelion ethanol fraction 13 g Example 9 Preparation Example 4 10 g of ethylmethyldichloromethane fraction Preparation Example 6 Agaricus mushroom water extract 10 g Preparation Example 10 Dandelion ethanol fraction 13 g Preparation Example 12 3 g of ginger powder Example 10 Preparation Example 4 10 g of ethyl acetate fraction Preparation Example 5 Agaricus mushroom ethanol extract 10 g Preparation Example 10 Dandelion ethanol fraction 13 g Preparation Example 13 Ginger hot water extract 6 g Example 11 10 g of the yulmu butanol fraction of Preparation Example 4 Preparation Example 5 Agaricus mushroom ethanol extract 10 g Preparation Example 10 Dandelion ethanol fraction 13 g Preparation Example 13 Ginger hot water extract 9 g Example 12 10 g of the yulmu butanol fraction of Preparation Example 4 Preparation Example 6 Agaricus mushroom water extract 10 g Preparation Example 10 Dandelion ethanol fraction 13 g Preparation example 11 Glycyrrhizin water extract 5 g Example 13 Preparation Example 1 Yulmu powder 10 g Preparation Example 5 Agaricus mushroom ethanol extract 10 g Preparation Example 8 Pine needle leaf hexane fraction 15 g Preparation example 11 Glycyrrhizin water extract 5 g Example 14 Preparation Example 1 Yulmu powder 10 g Preparation Example 6 Agaricus mushroom water extract 10 g Preparation Example 7 Pine needles water extract 16g Preparation Example 12 ginger powder 8 g Example 15 10 g of yu-mu ethanol extract of Preparation Example 2 Preparation Example 5 Agaricus mushroom ethanol extract 10 g Preparation Example 10 Dandelion ethanol fraction 13 g Preparation Example 13 8 g of ginger powder Example 16 10 g of yu-mu ethanol extract of Preparation Example 2 Preparation Example 6 Agaricus mushroom water extract 10 g Preparation Example 12 ginger powder 8 g Preparation example 11 Glycyrrhizin water extract 5 g

Experimental Example  1. Cancer cells Proliferation inhibitory ability  Measure

The ability to inhibit the proliferation of cancer cells was measured by measuring cell viability, and specifically measured by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetra-zolium bromide (MTT) assay.

First, AGS human gastric adenocarcinoma cells were purchased from Korean Cell Line Bank (Seoul National University). Specifically, the cancer cell is 100 units / mL of penicillin-streptomycin (GIBCO, USA) and 10% fetal bovine serum (FBS) is contained 37 ℃ using RPMI 1640 _{medium, 5% CO 2 incubator (model311} , Forma , USA). Each cultured cancer cell was refeeded 2-3 times a week, washed with PBS in 6-7 days, and cells attached with 0.05% trypsin-0.02% EDTA were separated and centrifuged . After that, the culture medium was added to the integrated cancer cells, and the cells were well mixed to uniformly disperse the cancer cells with a pipette and used for the experiment while subculturing in a cell culture flask.

The cultured cancer cells were dispensed in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well in a volume of 180 μL, and the components of Preparation Examples 1 to 13 and Examples 1 to 15 were dissolved in a concentration of 10 μM, 30 μM, 50 μM, 100 μM, 200 μM or 350 μM . The cells were added to each well and incubated at 37 ° C in a 5% CO ₂ incubator for 72 hours. To this, 20 μL of MTT solution prepared at a concentration of 5 mg / mL was added to phosphate buffer saline (PBS) and cultured for 4 hours in the same culture condition. The supernatant was then removed and 150 μL of DMSO was added to each well. After stirring for 30 minutes, the absorbance was measured at 540 nm with an ELISA reader. The measured cell viability results are shown in Figs. 1 to 5 and Table 2.

Cancer cell survival rate (% of control.) 10 μM 30 μM 50 μM 100 [mu] M 200 μM 350 μM Example 1 99 95 88 75 70 67 Example 2 98 93 84 79 71 63 Example 3 96 92 82 77 63 61 Example 4 95 90 77 65 61 59 Example 5 97 91 79 63 55 51 Example 6 96 93 78 70 64 51 Example 7 95 91 78 69 61 52 Example 8 98 89 80 71 65 50 Example 9 99 89 75 63 51 46 Example 10 96 85 74 59 50 45 Example 11 95 83 71 58 49 41 Example 12 95 83 70 65 57 50 Example 13 99 92 79 72 61 55 Example 14 97 84 80 68 55 52 Example 15 95 86 74 61 50 44 Example 16 95 88 79 66 57 51 Preparation Example 1 100 99 97 94 93 91 Preparation Example 2 99 98 93 90 90 88 Preparation Example 3 99 96 91 88 85 83 Preparation Example 4 (Hexane) 98 94 88 85 83 81 Preparation Example 5 98 91 86 83 81 80 Preparation Example 6 97 95 93 92 88 83 Preparation Example 7 98 95 93 90 88 86 Preparation Example 8
(Hexane) 97 94 92 90 87 85 Preparation Example 9 99 95 91 89 84 78 Preparation Example 10
(Hexane) 97 95 91 87 80 75 Preparation Example 11 96 95 92 88 87 85 Preparation Example 12 94 93 90 88 85 83 Preparation Example 13 95 92 90 85 83 80

As can be seen from Figs. 1 to 3 and Table 2, it was confirmed that the mixture of Examples 1 to 15 improved cancer cell proliferation inhibition effect in a concentration-dependent manner.

On the other hand, as shown in Figs. 1 to 5 and Table 2, the components prepared in Preparative Examples 1 to 13 showed a cancer cell proliferation inhibitory effect in a dose-dependent manner. However, .

In particular, the survival rates of cancer cells in Examples 6 to 8 were lower than those of Examples 3 to 5, and it was found that the mixture of yulmu, agaricus mushroom and dandelion showed better effects. In addition, it can be confirmed that the best effect is obtained in Examples 8 to 8 among Examples 6 to 8, which is superior to the case where a mixture of yulmu, agaricus mushroom and dandelion is mixed at a weight ratio of 1: 0.8: 1.3 .

In addition, in the case of Examples 9 to 11 and Example 15, it was confirmed that the cancer cell survival rate was the lowest by incorporating ginger into the health functional food prepared by mixing at the above weight ratio, It can be seen that the health functional food exhibits the best efficacy when it contains yulmu, agaricus mushroom, dandelion and ginger in a weight ratio of 1: 0.8 ~ 1: 1 ~ 1.5: 0.6 ~ 0.9.

In addition, it can be confirmed that the tumor cell survival rate is also excellent in Example 12, and it can be seen that even when containing yulmu, agaricus mushroom, dandelion and glycyrrhizin, it shows excellent efficacy.

As can be seen from Preparation Examples, Examples and Experimental Examples, the mixture of the present invention contains extracts derived from natural or natural products and has no toxicity and high antitumor activity, thus providing a health functional food suitable for tumor prevention or improvement It was confirmed that there is an effect to be achieved.

Hereinafter, a preparation example of a health functional food containing a composition according to a preferred embodiment of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

< Manufacturing example >

(Vitamin A acetate 70 ㎍, Vitamin E 1.0 mg, Vitamin B 1 0.13 mg, Vitamin B 2 0.15 mg, Vitamin B 6 0.5 mg, Vitamin B 12 0.2 쨉 g, (1.75 mg of ferrous sulfate, 0.82 mg of zinc oxide, 25.3 mg of magnesium carbonate, 15.1 mg of potassium phosphate monobasic, 15 mg of biotin, 10 μg of biotin, 1.7 mg of nicotinic acid amide, 50 μg of folic acid and 0.5 mg of calcium pantothenate) Mg of calcium phosphate, 55 mg of calcium phosphate, 90 mg of potassium citrate, 100 mg of calcium carbonate, and 24.8 mg of magnesium chloride) were mixed to prepare a granule, and a health food was prepared according to a conventional method.

Claims (10)

An ethanol extract of Agaricus mushroom, an ethanol fraction of dandelion ethanol extract, and a mixture of hot water extract of ginger, as an active ingredient,
Wherein the mixture comprises 90 to 100 parts by weight of an agaricus mushroom ethanol extract, 120 to 150 parts by weight of a dandelion ethanol fraction, and 80 to 90 parts by weight of a ginger hot water extract, based on 100 parts by weight of the urumu butanol fraction,
Wherein the concentration of the mixture is 200 μM to 350 μM.
delete The method according to claim 1,
Wherein the health functional food is a dairy product including beef, bread, chocolate, candy, snack, pizza, noodles, gum, ice cream, a drink, an alcoholic beverage or a vitamin complex
Health functional food for gastric cancer prevention.

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