KR101757358B1 - Apparatus for manufacturing polymer biochip - Google Patents

Apparatus for manufacturing polymer biochip Download PDF

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Publication number
KR101757358B1
KR101757358B1 KR1020150157355A KR20150157355A KR101757358B1 KR 101757358 B1 KR101757358 B1 KR 101757358B1 KR 1020150157355 A KR1020150157355 A KR 1020150157355A KR 20150157355 A KR20150157355 A KR 20150157355A KR 101757358 B1 KR101757358 B1 KR 101757358B1
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KR
South Korea
Prior art keywords
polymer
guide member
body member
biochip
guide
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KR1020150157355A
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Korean (ko)
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KR20170054776A (en
Inventor
곽봉섭
정기수
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한국기계연구원
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Priority to KR1020150157355A priority Critical patent/KR101757358B1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G77/00—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule
    • C08G77/04—Polysiloxanes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The polymeric biochip manufacturing apparatus of the present invention comprises a body member to which a master wafer on which a biochip pattern is formed is fixed, a detachable body member, a portion corresponding to the master wafer is formed to be open, A guide member extending from the inner side surface toward the center in a state where the guide member is in contact with an upper surface of the body member, and a cover member sealing the opened portion of the guide member.

Description

[0001] Apparatus for manufacturing polymer biochip [0002]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a polymer biochip manufacturing apparatus, and more particularly, to a polymer biochip manufacturing apparatus that can be used for manufacturing a biochip.

A biochip is a biological microchip that can analyze DNA defects, protein distribution, and reaction patterns by combining biological molecules such as DNA and protein on a small substrate.

Such biochips are attracting attention and are rapidly spreading in the field of scientific research, new drug development process, and clinical diagnosis.

Biochips can be divided into microarrays and microfluidics chips.

A microarray is a biochip capable of analyzing the binding behavior of a substance to be assayed by arranging thousands or tens of thousands of DNAs or proteins at regular intervals. The DNA chip, the protein chip, etc. This is representative.

A microfluidics chip is a microfluidic chip that implements microchannels, microchambers, mixers, and valves on a chip to control microfluidics, immobilizes biochemicals on a detection unit, and then uses the microfluidic flow to detect biochemicals to be detected. , Which is a system that detects the presence or absence of a reaction by reacting with an immobilized biochemical material. This is one of the most actively researched fields in the long term and in recent miniaturization trends.

Generally, a microfluidics chip-type biochip is prepared by directly attaching a guide made of a guide plastic or a silver foil to a silicon wafer having a biochip pattern formed thereon, and then introducing the liquid polymer into the guide so that the liquid polymer is in a solid state , It is made by separating the solid state polymer in which the biochip pattern is transferred from the silicon wafer.

However, when the guide made of plastic or silver foil for guiding is used, a bending force is applied to the silicon wafer in the process of detaching the polymer, and the silicon wafer may be destroyed during repeated use, rendering it impossible to use. In addition, when the thermosetting polymer is desorbed from the silicon wafer, the desorption force is not uniformly applied, and the polymer may be torn.

In addition, polymer is exposed to air during the process of making dam by using silver foil, for removing air of polymer, and during heat-hardening process, etc., Can be a major cause of death.

An embodiment of the present invention is to provide a polymer bio chip manufacturing apparatus capable of preventing breakage of a polymer and a silicon wafer in a polymer desorption process.

Also, an embodiment of the present invention is to provide a polymer bio chip manufacturing apparatus in which dust can be prevented from being inflow from the outside during a bio chip manufacturing process.

A polymer biochip manufacturing apparatus according to one aspect of the present invention includes a body member to which a master wafer on which a biochip pattern is formed is fixed, a detachable body member, a portion corresponding to the master wafer is formed to be open, A guide member extending from the inner side surface toward the center in a state of being coupled to the body member and including an extension portion formed to be in contact with the upper surface of the body member and a cover member sealing the opened portion of the guide member.

Meanwhile, the guide member may include at least one through-hole penetrating in the up-and-down direction, and a fastening member passing through the through-hole of the guide member and fastened to the body member.

Meanwhile, the cover member may be made of a transparent material.

Meanwhile, the cover member may include a sound pressure generator having a connection portion penetrating in the up and down direction, and connected to the connection portion, so that the cover member allows the internal space to be in a negative pressure state while the guide member is closed.

The body member may further include a first sealing member interposed between the body member and the guide member.

The cover member may further include a second sealing member interposed between the cover member and the body member.

The apparatus for manufacturing a polymer bio chip according to an embodiment of the present invention includes a guide member. The extended portion of the guide member stably supports the edge of the solid state polymer while the guide member is separated from the body member. Therefore, the solid state polymer can be easily detached from the master wafer without tearing.

In addition, the apparatus for manufacturing a polymer bio chip according to an embodiment of the present invention is capable of preventing dust from entering from the outside by closing the internal space.

1 is a perspective view illustrating a polymer bio chip manufacturing apparatus according to an embodiment of the present invention.
2 is an exploded perspective view of the polymer-based biochip manufacturing apparatus shown in Fig.
Fig. 3 is a view showing the interior of the polymer biochip manufacturing apparatus shown in Fig. 1. Fig.
4 is a perspective view illustrating a polymer bio chip manufacturing apparatus according to another embodiment of the present invention.
FIG. 5 is a view showing a state in which a polymer in a solid state is separated from a guide member in the polymer-based biochip manufacturing apparatus of FIG. 4;

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein.

In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

In addition, in the various embodiments, elements having the same configuration are denoted by the same reference numerals and only representative embodiments will be described. In other embodiments, only the configurations other than the representative embodiments will be described.

Throughout the specification, when a part is referred to as being "connected" to another part, it includes not only "directly connected" but also "indirectly connected" between other parts. Also, when a part is referred to as "including " an element, it does not exclude other elements unless specifically stated otherwise.

FIG. 1 is a perspective view illustrating a polymer biochip manufacturing apparatus according to an embodiment of the present invention, FIG. 2 is an exploded perspective view of the polymer biochip manufacturing apparatus shown in FIG. 1, and FIG. 3 is a cross- 1 is a diagram showing the inside of a chip manufacturing apparatus.

1 to 3, an apparatus 100 for manufacturing a polymer bio chip according to an embodiment of the present invention includes a body member 110, a guide member 120, and a cover member 130.

In the body member 110, the master wafer 11 on which the biochip pattern 11a is formed is fixed. The bio chip pattern 11a may include at least one of a microchannel, a micro chamber, a mixer, and a valve. The master wafer 11 may be fixed to the body member 110 by means of an adhesive but is not limited thereto and any method can be used as long as the master wafer 11 can be fixed to the body member 110 have.

Meanwhile, the body member 110 may be made of a thermoplastic resin having heat resistance. The material of the body member 110 may be, for example, polycarbonate.

The guide member 120 is detachably attached to the body member 110. A portion of the guide member 120 corresponding to the master wafer 11 is formed to be open.

The guide member 120 may be made of a thermoplastic resin having heat resistance. For example, the material of the guide member 120 may be polycarbonate.

This guide member 120 includes an extension 121. The extension part 121 is formed to extend from the inner side to the center in a state where the guide member 120 is coupled to the body member 110 and to be in contact with the upper surface of the body member 110.

The shape of the extension part 121 may be formed to be inclined downwardly away from the inner surface of the guide member 120, for example. As another example, the shape of the extension portion 121 may be a plate-like shape having a predetermined thickness.

The extension portion 121 may be formed so as not to interfere with the master wafer 11. [ That is, the upper surface of the body member 110 may be formed to correspond to an area where the master wafer 11 is not positioned.

Between the guide member 120 and the body member 110 having such a structure, an internal space capable of accommodating a liquid polymer (not shown) used for forming a polymer biochip can be formed.

Here, the liquid polymer (not shown) may include polydimethylsiloxane (PDMS), for example.

The cover member (130) seals the open portion of the guide member (120). For this purpose, the cover member 130 may have a shape corresponding to the open portion of the guide member 120.

Meanwhile, the cover member 130 may be made of a transparent material. The material of the cover member 130 may be, for example, transparent plastic or glass.

The guide member 120 may include at least one through hole 122. The guide member 120 may be formed in the body member 110. The guide member 120 may include at least one through hole 122. [ The through hole 122 is formed in the guide member 120 so as to penetrate in the vertical direction.

Here, the apparatus 100 for manufacturing a polymer bio chip according to an embodiment of the present invention may include a fastening member 140. The fastening member 140 passes through the through hole 122 of the guide member 120 and is fastened to the body member 110. The fastening member 140 may be, for example, a bolt.

Meanwhile, the apparatus 100 for manufacturing a polymer-based chip according to an embodiment of the present invention may further include a first sealing member 150.

The first sealing member 150 is interposed in a portion where the body member 110 and the guide member 120 are in contact with each other.

The apparatus 100 for manufacturing a polymer bio chip according to an embodiment of the present invention may further include a second sealing member 160. The second sealing member 160 is interposed in a portion where the cover member 130 and the body member 110 are in contact with each other.

4 is a perspective view illustrating a polymer bio chip manufacturing apparatus according to another embodiment of the present invention.

Referring to FIG. 4, in the polymer bio chip manufacturing apparatus 100 according to another embodiment of the present invention, the cover member 130 may be formed with a connecting portion vertically penetrating therethrough.

The apparatus 100 for manufacturing a polymer-based chip according to another embodiment of the present invention may include a sound pressure generator 170. The sound pressure device 170 is connected to the connection portion 132 so that the inner space of the sound pressure device 170 is in a state of being in a sound pressure state when the cover member 130 hermetically seals the guide member 120. For this purpose, the sound pressure device 170 may include a connector. The connector is connected to the connection part (132).

In the polymer bio chip manufacturing apparatus 100 according to another embodiment of the present invention having such a structure, a liquid polymer (not shown) may be formed only by connecting the sound pressure device 170 to the connection portion of the cover member 130, It is possible to quickly remove the air bubbles inside.

Hereinafter, a process of manufacturing a biochip by the polymer biochip manufacturing apparatus 100 according to an embodiment of the present invention will be described.

The guide member 120 is coupled to the body member 110. [ The liquid polymer is injected into the inner space formed by the guide member 120 so that the master wafer 11 is completely locked.

Next, the cover member 130 is engaged with the guide member 120 to seal the inner space. Then, air bubbles in the polymer solution 10 are removed by using the sound pressure device 170.

Next, the liquid polymer is cured. As a method for curing the polymer, for example, a method using an electric heater may be used, but the method is not limited thereto, and it may be possible to cure the polymer over time.

Finally, when the portion of the solid state polymer 10 that has been manufactured is transferred and the biochip pattern is transferred, the polymer biochip can be obtained.

FIG. 5 is a view showing a state in which a polymer in a solid state is separated from a guide member in the polymer-based biochip manufacturing apparatus of FIG. 4;

5, in the process of separating the guide member 120 from the body member 110 (see FIG. 2), the extension portion 121 of the guide member 120 is separated from the solid polymer 10) can apply a uniform force to the entire edge.

Referring back to FIG. 2, the polymer bio chip manufacturing apparatus 100 according to an embodiment of the present invention includes a guide member 120. The extension part 121 of the guide member 120 stably supports the edge of the polymer 10 in the solid state while the guide member 120 is separated from the body member 110. Therefore, the polymer 10 in a solid state can be easily detached from the master wafer 11 without tearing.

In addition, the apparatus 100 for manufacturing a polymer-based biochip according to an embodiment of the present invention can prevent dust from entering from the outside by closing the internal space.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, And are not used to limit the scope of the present invention described in the scope. Therefore, those skilled in the art will appreciate that various modifications and equivalent embodiments are possible without departing from the scope of the present invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

10: polymer 11: master wafer
100: polymer bio chip manufacturing apparatus 110: body member
120: guide member 121:
130: cover member 140: fastening member
150: first sealing member 160: second sealing member
170: Sound pressure machine

Claims (6)

A body member on which a master wafer on which a biochip pattern is formed is fixed,
A portion corresponding to the master wafer is formed to be open and filled with a polymer and is extended from the inner side to the center in a state of being coupled to the body member, A guide member formed to contact and including an extension for supporting a lower portion of the polymer,
And a cover member for sealing the opened portion of the guide member. The method according to claim 1,
Wherein at least one through-hole penetrating in the up-and-down direction is formed in the guide member,
And a fastening member passing through the through-hole of the guide member and fastened to the body member. The method according to claim 1,
Wherein the cover member is made of a transparent material. The method according to claim 1,
The cover member is formed with a connecting portion penetrating in the vertical direction,
And a sound pressure unit connected to the connection unit to allow the interior space to be in a negative pressure state in a state in which the cover member hermetically seals the guide member. The method according to claim 1,
Further comprising a first sealing member interposed in a portion where the body member and the guide member are in contact with each other. The method according to claim 1,
And a second sealing member interposed in a portion where the cover member and the body member are in contact with each other.
KR1020150157355A 2015-11-10 2015-11-10 Apparatus for manufacturing polymer biochip KR101757358B1 (en)

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KR101757358B1 true KR101757358B1 (en) 2017-07-12

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005053143A (en) * 2003-08-06 2005-03-03 Apic Yamada Corp Resin molding method and resin molding apparatus
JP2015107883A (en) * 2013-12-03 2015-06-11 コニカミノルタ株式会社 Method and apparatus for manufacturing glass molding

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005053143A (en) * 2003-08-06 2005-03-03 Apic Yamada Corp Resin molding method and resin molding apparatus
JP2015107883A (en) * 2013-12-03 2015-06-11 コニカミノルタ株式会社 Method and apparatus for manufacturing glass molding

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