KR101741766B1 - Apparatus for capturing images of blood cell - Google Patents
Apparatus for capturing images of blood cell Download PDFInfo
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- KR101741766B1 KR101741766B1 KR1020160109890A KR20160109890A KR101741766B1 KR 101741766 B1 KR101741766 B1 KR 101741766B1 KR 1020160109890 A KR1020160109890 A KR 1020160109890A KR 20160109890 A KR20160109890 A KR 20160109890A KR 101741766 B1 KR101741766 B1 KR 101741766B1
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Abstract
According to one aspect of the present invention, there is provided a blood cell image capturing apparatus comprising: a camera section including at least one camera; a lens section including a plurality of lenses having different magnifications; A support for supporting the blood smear slide, a lens and a lens in an aligned state, a transfer part for adjusting a relative position of the blood smear slide, a light source for providing illumination, And a control section for controlling the camera section, the lens replacement section, the transfer section, and the illumination section, respectively, to obtain a predetermined image from the blood smeared on the slide.
Description
TECHNICAL FIELD The present invention relates to a blood cell image capturing apparatus.
An image analyzer (IA: Image Analyzer) is an apparatus for imaging and analyzing blood cells contained in blood-smear blood samples. Particularly, the image analyzer can measure the blood cell image captured through WBC (White Blood Cell) analysis by five kinds, basophil, neutrophil, acidic white blood cell (Eosinophil), mononuclear leukocyte (Monocyte) Lymphocyte (Lymphocyte) is classified as a device to distinguish.
Actually, leukocytes may exist in various forms depending on the patient's condition, and the hematocrit counting the number of each type is very important for diagnosing the condition of the patient, diagnosis and tracking of the disease.
Therefore, various methods are being developed to improve the accuracy of the image analyzer's discrimination results.
An object of the present invention is to provide a blood cell image capturing apparatus capable of improving an image capturing speed and an image quality.
According to an aspect of the present invention, there is provided a camera system including a camera unit including at least one camera, a lens unit including a plurality of lenses having different magnifications, A lens replacement portion for positioning the lens, a support for placing the blood smear slide, a camera and lens in an aligned state, a transfer portion for adjusting the relative position of the blood smear slide, and a light source for providing illumination. And a control section for controlling the camera section, the lens replacement section, the transfer section, and the illumination section to obtain a predetermined image from the blood smearing on the slide, respectively.
Further, the illumination unit may include a collimating lens and a collimating lens arranged to sequentially pass the light irradiated from the light source.
Further, the camera section may include a camera for photographing and a camera for auto-focusing.
Further, the lens portion may include a low magnification lens and a high magnification lens.
Further, the control unit may be provided to capture a first region of blood smeared on the slide with a low magnification lens, and to generate coordinate values of blood cells appearing in the image of the first region.
In addition, the control unit can select one or more necessary regions for obtaining a high-resolution image of the blood cells in the first region, and align the high magnification lens with respect to the camera in order to image the selected required region with the high magnification lens.
In addition, the control unit may be provided to match the center coordinate values of the blood cells in the first area and the necessary area.
In addition, the control unit may control to sequentially capture images through the high magnification lens along predetermined trajectories connecting the plurality of required areas.
As described above, the hemocyte image pickup apparatus according to at least one embodiment of the present invention can improve the image pickup speed and image quality.
1 is a conceptual diagram illustrating a blood cell differentiation system according to an embodiment of the present invention.
2 is a configuration diagram illustrating a blood-cell image capturing apparatus according to an embodiment of the present invention.
Figs. 3 to 5 are views showing some components of a blood cell image capturing apparatus according to an embodiment of the present invention. Fig.
FIG. 6 through FIG. 9 are views for explaining a blood image processing method according to an embodiment of the present invention.
FIGS. 10 to 11 are views for explaining an image capturing method of a blood smear sample according to an embodiment of the present invention.
Hereinafter, a blood cell differentiation system, a blood cell image imaging device, a blood image processing method, and an image capturing method of a blood smear sample according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.
In addition, the same or corresponding reference numerals are given to the same or corresponding reference numerals regardless of the reference numerals, and redundant description thereof will be omitted. For convenience of explanation, the size and shape of each constituent member shown in the drawings are exaggerated or reduced .
Fig. 1 is a conceptual diagram showing a blood
The blood cell differentiation system includes an image analyzer (IA) 100 and a manual review center (MRC) In this document, 'blood cells' may mean red blood cells or white blood cells, but for convenience of explanation, 'blood cells' refers to 'white blood cells' as an example.
The
The
In addition, the patient information (also referred to as "meta information") may include one or more of age, sex, white blood cell count, red blood cell count, hemoglobin count, hematocrit count and platelet count.
In addition, the
Specifically, the
Particularly, the
In one embodiment, the
Specifically, the
The
In addition, the
On the other hand, the
In addition, the
In addition, the
In addition, the
Fig. 2 is a configuration diagram showing a hemocyte
As described above, the
2, the hemocyte
Specifically, the blood cell
The
In addition, the
The
The
In addition, the
In addition, the
In addition, the
Hereinafter, a blood image processing method using the blood cell
FIG. 6 through FIG. 9 are views for explaining a blood image processing method according to an embodiment of the present invention.
The blood image processing method includes a first imaging step of acquiring an image (1000) of an entire area of blood smeared on a slide with a low magnification lens (221), and a second imaging step of obtaining, based on the distribution of blood cells in the image A selection step of selecting one or more necessary areas for acquiring a
Further, the correcting step may include matching the center coordinate values of the blood cells in the whole area and the necessary area. Referring to FIG. 7, FIG. 7A shows a
6, in the selecting step, the number of blood cells in each divided
In addition, the second imaging step may include a step of sequentially taking an image through the high magnification lens along a predetermined locus 313 (see Fig. 9) that successively connects the plurality of necessary areas. That is, by adjusting the relative positions of the camera and the lens in the alignment state and the blood smear slide through the above-described transfer unit, it is possible to continuously capture a high magnification image of blood cells.
Further, each imaging step may include the step of irradiating light from the
Further, as described above, each imaging step can be carried out through a CMOS camera.
Referring to FIG. 8, the blood image processing method may further include a generation step of generating an
For example, the
The above-described
6 and 9, a blood image processing method includes capturing an entire region of the
FIGS. 10 to 11 are views for explaining an image capturing method of a blood smear sample according to an embodiment of the present invention.
Referring to FIGS. 9 and 10, when the
The image capturing method of the
Further, the image capturing method of the blood smear sample may further include a selection step of selecting a plurality of
In addition, the image capturing method of the blood smear sample may further include an image capturing step of performing image capturing through a high magnification lens along a
On the other hand, the whole image of the blood smear sample can be obtained by obtaining a partial image for some areas of the blood smear sample, and merging the partial images.
Further, partial images may be distinguished by a first grid line (e.g., 300 in FIG. 6) on the entire image. Alternatively, the required area may be distinguished by a first grating line (e.g., 300 in FIG. 6) and a second grating line (e.g., 300 in FIG. 11).
Also, the required
The foregoing description of the preferred embodiments of the present invention has been presented for purposes of illustration and various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention, And additions should be considered as falling within the scope of the following claims.
1: hemocyte differentiation system
10: Manual Review Center
100: Image Analyzer
200: hemocyte image pickup device
210:
220:
230: Support
240:
250:
300: Grid line
310:
311: imaging effective area
312: imaging invalid area
313: Trajectory
400: blood smear slide
1000: Low magnification image
1001: High magnification image
1002: Image for discrimination
Claims (8)
A lens unit including a plurality of lenses having different magnifications;
A lens replacement unit for positioning a specific lens among the plurality of lenses to be aligned with the camera;
A support on which the blood smear slide is seated;
A camera and a lens in an aligned state, and a transfer unit for adjusting a relative position of the blood smear slide;
An illumination unit including a light source for providing illumination; And
And a control unit for controlling the camera unit, the lens replacement unit, the transfer unit, and the illumination unit to obtain a predetermined image from the blood smeared on the slide,
The lens portion includes a low magnification lens and a high magnification lens,
The control section is adapted to image a first region of blood smeared on the slide with a low magnification lens and to generate coordinate values of blood cells appearing in an image of the first region,
The control unit selects at least one necessary region for obtaining a high resolution image of the blood cells in the first region and aligns the high magnification lens with respect to the camera in order to image the selected required region with the high magnification lens,
And the control unit is arranged to match the center coordinate value of the blood cell in the first area and the necessary area.
And the illuminating unit includes a collimating lens and a collimating lens arranged so as to sequentially pass the light irradiated from the light source.
Wherein the camera section includes a camera for imaging and an auto focusing camera.
And the control unit controls imaging so that the imaging through the high magnification lens is sequentially performed along the predetermined trajectory connecting the plurality of necessary areas.
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KR20150129449 | 2015-09-14 | ||
KR1020150129449 | 2015-09-14 |
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KR1020160109891A KR101741765B1 (en) | 2015-09-14 | 2016-08-29 | Cytometry system |
KR1020160109892A KR101741764B1 (en) | 2015-09-14 | 2016-08-29 | Image precessing method of blood |
KR1020160109890A KR101741766B1 (en) | 2015-09-14 | 2016-08-29 | Apparatus for capturing images of blood cell |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20190022027A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Cytometry apparatus and method |
KR20190022026A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Cytometry apparatus and method |
KR20190022028A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Apparatus for capturing images of blood cell |
KR20190023886A (en) | 2017-08-30 | 2019-03-08 | (주)뉴옵틱스 | Apparatus for capturing images of blood cell and image analyzer with the same |
KR20200030959A (en) | 2018-09-13 | 2020-03-23 | (주)뉴옵틱스 | High speed image capture apparatus and method |
KR20200030958A (en) | 2018-09-13 | 2020-03-23 | (주)뉴옵틱스 | Auto focus apparatus and mehtod for image analysis |
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KR102576041B1 (en) * | 2021-02-09 | 2023-09-07 | (주)유아이엠디 | Acquisition method of high magnification image of slide sample |
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2016
- 2016-08-29 KR KR1020160109891A patent/KR101741765B1/en active IP Right Grant
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KR100843620B1 (en) * | 2007-01-11 | 2008-07-03 | 주식회사 코윈디에스티 | Microscope unit for the inspection / processing, and, a method |
JP2013221960A (en) * | 2012-04-13 | 2013-10-28 | Nikon Corp | Adjustment method for microscope, and microscope |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20190022027A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Cytometry apparatus and method |
KR20190022026A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Cytometry apparatus and method |
KR20190022028A (en) | 2017-08-25 | 2019-03-06 | (주)뉴옵틱스 | Apparatus for capturing images of blood cell |
KR20190023886A (en) | 2017-08-30 | 2019-03-08 | (주)뉴옵틱스 | Apparatus for capturing images of blood cell and image analyzer with the same |
KR20200030959A (en) | 2018-09-13 | 2020-03-23 | (주)뉴옵틱스 | High speed image capture apparatus and method |
KR20200030958A (en) | 2018-09-13 | 2020-03-23 | (주)뉴옵틱스 | Auto focus apparatus and mehtod for image analysis |
Also Published As
Publication number | Publication date |
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KR20170032176A (en) | 2017-03-22 |
KR101741765B1 (en) | 2017-05-31 |
WO2018043958A1 (en) | 2018-03-08 |
KR20170032175A (en) | 2017-03-22 |
KR20170032177A (en) | 2017-03-22 |
KR101741764B1 (en) | 2017-05-31 |
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