KR101735341B1 - An antibacterial composition comprising an extract of corylopsis coreana - Google Patents

Abstract

The present invention relates to a composition for preventing or treating pathogenic microorganisms, particularly, diseases caused by infection with an antibiotic-resistant infectious microorganism, which comprises a herbal extract as an active ingredient. In particular, the herbal extract, which is an active ingredient of the present invention, is an infectious microorganism, especially Methicillin-Resistant Staphylococcus aureus ) and vancomycin-resistant Staphylococcus (Vancomycin-Resistant Staphylococcus aureus ), as well as being free from cytotoxicity and being derived from natural materials, so that it is not problematic in terms of side effects, so that the treatment, improvement or prevention of diseases caused by microbial infection Can be widely used.

Description

AN ANTIBACTERIAL COMPOSITION COMPRISING AN EXTRACT OF CORYLOPSIS COREANA [0002]

The present invention relates to an antimicrobial composition comprising a heather extract as an active ingredient.

Antibiotics are generally referred to as antimicrobial agents in general, and in particular, substances which have antimicrobial activity against bacteria, in particular, agents that inhibit systems for synthesizing cell walls and proteins, it means. Antibiotics are predominantly extracted from molds and are used today to treat diseases caused by bacterial infections.

The most common antibiotic is penicillin manufactured by the British physician Alexander Fleming in 1928. Penicillin was the first antibiotic manufactured by mankind to respond to bacteria in earnest. A representative antibiotic developed after penicillin is methicillin, which is considered to be more effective than penicillin. Methicillin was made by partially modifying the chemical structure of penicillin.

The methicillin is classified as a beta-lactam class of antibiotics. The beta-lactam antibiotic binds to penicillin-binding proteins (PBPs) And thus the drug efficacy is exerted.

Antibiotic-resistant bacteria refer to bacteria that are resistant to certain antibiotics and that do not respond to antibiotics. For example, penicillin-resistant Staphylococcus aureus, which has no effect on penicillin as described above, is applicable. In addition, methicillin-resistant Staphylococcus , which has been reported to academia for the first time in 1961 and has become a major infectious disease in hospitals worldwide since then aureus , and MRSA) have been reported. The MRSA has been found to have a unique gene that is resistant to penicillin or methicillin antibiotics.

It is reported that the MRSA is mainly infected to a healthy person, mainly to a patient having weak immunity or a patient who has undergone surgery, but in case of infection, it causes sepsis or pneumonia to die.

Recently, healthy people are increasingly infected with MRSA. The US medical community or public health academics are concerned about the increased infections caused by pathogens (multidrug resistant pathogens) that do not respond to many antibiotics such as MRSA. For example, the spread of Staphylococcus aureus in Alaska indigenous people in 1984 and the spread of skin infections in its residents in August 2000.

This confirms that MRSA can be transmitted not only from the patient in the hospital, but also from public life in the community, as is conventionally known. In Korea, about 40 children in Gyeongnam area were reported to have been infected with MRSA during the last two years. Children who were treated for infectious staphylococcal cutaneous skin syndrome were re-examined by Seoul National University's Department of Diagnostic Medicine and were confirmed as MRSA infection. Have not been admitted to the hospital or have taken antibiotics, and are estimated to have been infected with MRSA from the surrounding environment of the community.

According to the Korea Food & Drug Administration (KFDA) and the Center for Disease Control and Prevention, the antimicrobial resistance of 10 university hospitals nationwide, including Seoul National University Hospital, was 10% higher than the previous year. , And the proportion of MRSA is reported to be 71.6%, which is 10% higher than 61.2% in 2004.

In recent years, interest in natural antibiotics having antimicrobial activity against antibiotic-resistant strains has been increased as compared with other natural plants, especially natural products, which have been used as natural foods. Recently, various drugs or antibiotics using seaweed have been developed There is an active effort to do so.

10-0876922B 10-0729437B 10-2014-0009717A

Disclosure of the Invention The object of the present invention is to solve the problems of the prior art as described above, and it is an object of the present invention to provide an antimicrobial activity against common infectious strains, and more particularly to a method for producing methicillin-Resistant Staphylococcus The present invention also provides an antimicrobial composition comprising, as an active ingredient, a herbal extract having a strong antimicrobial activity against an antibiotic resistant strain including aureus .

In order to achieve the above object, the present invention provides an antimicrobial composition. The antimicrobial composition may preferably be an antimicrobial composition having an antimicrobial activity against an antimicrobial-resistant strain. The antimicrobial composition may be a pharmaceutical composition or a pharmaceutical composition.

The present invention also provides a composition for treating or preventing diseases caused by methicillin-resistant Staphylococcus aureus infection.

The present invention also provides a food composition for improving or preventing diseases caused by methicillin-resistant Staphylococcus aureus infection. The food composition may be a functional food composition.

The present invention also provides a composition for cleaning having antimicrobial activity, preferably an antimicrobial activity against an antibiotic resistant strain, more preferably an antimicrobial activity against a methicillin resistant Staphylococcus aureus.

Unless otherwise specified in the present specification, the effective ingredient means an antimicrobial effect, specifically, an antimicrobial effect on an antibiotic resistant strain, more specifically, an antimicrobial effect on a methicillin resistant Staphylococcus aureus &Quot;

As used herein, unless otherwise specified, pharmacologically acceptable means physiologically acceptable and does not cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like, when administered to an animal, preferably a human, do.

The inventors of the present invention have found that an antibiotic-resistant strain having an increased serious problem in relation to an infectious strain which is a causative agent of an infectious disease among the natural substances having a low side effect and easy to medicament, especially recently abuse of medicines and the like, specifically methicillin-resistant Staphylococcus aureus (Methicillin-Resistant Staphylococcus aureus , and MRSA), the herbicide ( Corylopsis coreana ), especially extracts of flower part of the herring have antibacterial activity against antibiotic resistant strains including methicillin resistant Staphylococcus aureus. The antimicrobial activity of these extracts is affected not only by the parts of the hair but also by the extraction solvent and fraction solvent , And it has a remarkably excellent antimicrobial effect upon fractionation of ethyl acetate unlike the other fraction solvents. The antimicrobial activity against these antibiotic resistant strains was confirmed by methicillin-resistant Staphylococcus aureus and Vancomycin-Resistant Staphylococcus aureus , VRSA), and thus the present invention has been completed.

Hereinafter, the present invention will be described in more detail.

The invention hieori (Corylopsis coreana ) flower extract as an active ingredient, methicillin-resistant Staphylococcus aureus ) and vancomycin-resistant Staphylococcus (Vancomycin-Resistant Staphylococcus aureus ) having an antimicrobial activity.

The Hereke flower extract may be the ethyl acetate fraction of a 70% ethanolic aqueous solution of the herbal flower.

In addition, the antimicrobial composition may be used in a variety of forms such as E. coli , Bacillus sp., Micrococcus sp., Staphylococcus sp., Enterococcus sp., Salmonella sp. bacteria (Salmonella sp.), when keulrep may be one having antimicrobial activity against Ella (Klebsiella sp.) and Pseudomonas bacteria (Pseudomonas. sp).

The present invention also relates to a method infection of at least one pathogenic microorganism selected from the group consisting of Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Staphylococcus aureus , which contains Coreana flower extract as an active ingredient. To a composition for preventing or treating diseases.

The disease caused by the infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus is selected from the group consisting of respiratory infections such as pneumonia, pulmonary pyogenosis, empyema, sepsis, bacteremia, infective endocarditis, Intestinal infectious disease, intractable pressure ulcer or wound infection, and more preferably, it may be any one selected from the group consisting of pneumonia, sepsis, bacteremia, infective endocarditis, and enteritis.

The present invention also relates to a method which comprises an antimicrobial composition having antimicrobial activity against Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Staphylococcus aureus , which comprises coreana flower extract as an active ingredient, To a cleaning composition.

The cleaning composition may be a detergent for clothes, a dishwashing detergent, a food cleaner or a household appliance cleaner.

The invention hieori (Corylopsis coreana ) extract as an active ingredient. The antimicrobial composition may preferably be an antimicrobial composition having an antimicrobial activity against an antimicrobial-resistant strain. The antimicrobial composition may be a pharmaceutical composition or a pharmaceutical composition.

The heron ( Corylopsis coreana ) is also known as a calligraphic plaster , and it is distributed in East Asia, rarely in North America or Central America. There are 29 species, 1 species in Japan and 5 species in India. One species is growing in Korea. Among them, the plants of heather ( Corylopsis coreana , Korean winter hazel). The herbarium was first discovered in 1910 in Sunchon Songgwang Temple in Chonnam Province. It is known to be native to the Jirisan region in Korea, and is a species registered as a special plant in the academic world in 1924. The herd is a deciduous broad-leaved shrub that grows up to 1 m to 5 m, has many shrubs on its stem, forms a cluster, and has a large group of closely-spaced buds with azaleas, zinnia, It is known to grow well in sunny places and to have strong cold resistance, so it does not wear the East Sea at temperatures below minus 30 degrees, and it is known to grow well on dry soil because of its strong weather resistance. The herb has been widely used as a garden water to see the yellow leaves that bloom in the early spring and the leaves of the yellow in autumn, but the growth rate is slow, but the biological activity and major constituents thereof are almost reported It is not.

The herring may be a ground part, a ground part or a mixture thereof, preferably a ground part, and more preferably a flower.

As described above, the alcoholic aqueous solution of the above-mentioned flowers, specifically the 60% to 80% aqueous alcoholic solution, more specifically, the aqueous 70% methanolic aqueous solution, may contain stem or roots having no antimicrobial activity against other areas, Compared with the extracts, the antimicrobial activity was remarkably excellent, and the antimicrobial activity against various antibiotic resistant strains was found to be excellent in terms of antibacterial effect.

The herbal extract of the present invention can be prepared by extracting with an extraction solvent or extracting with an extraction solvent and then fractionating the extract with a fraction solvent.

The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as methanol, ethanol or the like, an alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, a nonpolar solvent such as benzene, hexane or dichloromethane, or a mixed solvent thereof, preferably 1 to 5 carbon atoms More preferably 50 to 100% of an aqueous alcohol solution, more preferably 60 to 90% of an aqueous methanol solution may be selected from the group consisting of alcohols, water and mixtures thereof.

The herbal extract of the present invention may be one prepared by a conventional plant extract, specifically, a method for producing an on-land plant extract. More specifically, the method can be carried out by adding an extraction solvent to the herd having the impurities removed and performing the extraction process. The extraction process may be a cold extraction method, a warm extraction method, a pressure extraction method, or an ultrasonic pulverization extraction method.

The extract may be an extract prepared by the above-mentioned extraction method, specifically, a fraction obtained by further fractionating the crude extract. The fraction solvent may be a solvent selected from the group consisting of ethyl acetate, ether, chloroform, benzene, hexane, methylene chloride and a mixed solvent thereof, preferably ethyl acetate. Specifically, the fractionation step is performed by sequentially adding hexane, chloroform, ethyl acetate, butanol, and water to the crude extract in this order, then sequentially fractionating the hexane fraction, the chloroform fraction, the ethyl acetate fraction, the butanol fraction, . ≪ / RTI >

After the extraction or fractionation, the extract or fraction may be subjected to a vacuum filtration process or may be further concentrated and / or lyophilized to concentrate or remove the solvent. The obtained extract can be stored in a deep freezer until use.

Specifically, the herbal extract may be further subjected to a filtration step, a concentration step and / or a drying step after the solvent extraction step, either selectively or in duplicate. The filtration step may be performed using a filter paper, The process may be carried out through reduced pressure concentration or the like, and the drying process may be performed by a method such as hot air drying or freeze drying.

In this regard, the heather extract may preferably be a herring flower extract, more preferably a 70% methanol extract of Heather or an ethyl acetate fraction of 70% methanol extract of Heather.

In the present invention, the antimicrobial composition may have the same meaning as the antibiotic, which is generically referred to as an antimicrobial agent, and may have the same meaning as an antimicrobial agent, a bactericide, an antiseptic, a preservative or a bactericidal agent, Means a substance capable of inhibiting or inhibiting a living function.

The antimicrobial composition of the present invention may contain 0.001% by weight to 99.99% by weight, preferably 0.1% by weight to 99% by weight, of the above-mentioned Heather's extract based on the total weight of the composition. Accordingly, the content of the active ingredient can be appropriately controlled.

The antimicrobial composition may be an antimicrobial composition having an antimicrobial activity against a pathogenic microorganism or an infectious strain, specifically, a gram-positive microorganism or a gram-negative microorganism. More particularly, the antimicrobial composition may include methicillin-resistant Staphylococcus aureus (MRSA) vancomycin-resistant Staphylococcus aureus, the antimicrobial composition having an antimicrobial activity against (vancomycin-resistant Staphylococcus aureus, VRSA), preferably a methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, Escherichia coli (E. coli), Bacillus bacterium (Bacillus Micrococcus sp., Staphylococcus sp., Enterococcus sp., Salmonella sp., Klebsiella sp., and Pseudomonas sp. bacteria antimicrobial composition, more preferably from methicillin-resistant Staphylococcus aureus with anti-bacterial activity against (Pseudomonas. sp), Coma, who is resistant Staphylococcus aureus, Escherichia coli, Bacillus subtilis (Bacillus subtilis , Micrococcus luteus , Staphylococcus ( Staphylococcus aureus) aureus), Enterococcus faecalis (Enterococcus Faecalis), Salmonella typhimurium Moorim (Salmonella typhimrium ), Klebsiella pneumoniae or Klebsiella pneumonia , and Pseudomonas aeruginosa .

The methicillin-resistant Staphylococcus aureus is a pathogenic bacterium belonging to the gram-positive staphylococcus, which is resistant to double penicillin antibiotics such as methicillin, oxacillin or nafcillin . The methicillin-resistant Staphylococcus aureus is resistant to most beta-lactam class of antibiotics, including cephalosporins and imipenem, and is resistant to aminoglycosides, quinolone antibiotics, It is common for most antibiotics to be resistant.

The methicillin-resistant Staphylococcus aureus has been reported as a cause of severe infections such as pneumonia and septicemia as well as skin infections such as abscess or wound infection. In order to treat infections caused by these diseases, vancomycin or teicoplanin It has been reported that very limited antibiotics such as teicoplanin can be used.

It has been reported that infections caused by methicillin-resistant Staphylococcus aureus cause respiratory infections such as pneumonia, pulmonary emphysema and empyema, sepsis, bacteremia, infective endocarditis, intestinal infections, intractable pressure ulcers or wound infections.

Since the above-mentioned methicillin-resistant Staphylococcus aureus can settle in any part of the body and cause infection, it is possible to infect not only by contact transmission but also by airborne transmission, It has been reported that medical personnel who have been hospitalized for long periods of time may be infected with airborne infections because of their high rate of nasal passages.

The above-mentioned Escherichia coli is a bacterium which lives in the intestines of a person or an animal. Especially, Escherichia coli exists in the large intestine and is called a Escherichia coli, and causes a disease in a region other than the intestine. Specifically, it is a pathogen that can cause cystitis, pyelitis, peritonitis or sepsis , And Escherichia coli causing infectious diarrhea is referred to as a pathogenic Escherichia coli.

The pneumococcal bacterium is a pathogenic bacterium that causes pneumonia and refers to gram negative bacillus.

The antimicrobial composition of the present invention can be applied directly to animals including humans to exhibit antimicrobial activity. The antimicrobial composition exhibits an antimicrobial effect against the harmful strain, that is, the infectious strain, and prevents the infection by the infectious strain, It is possible to effectively control the strain and, when applied to an animal ingested by a human, it is possible to prevent a secondary infection of a human by an infected animal.

The animal is an organism corresponding to a plant and mainly consumes organic matter as nutrients and refers to the digestion, reproduction and differentiation of the respiratory organs. Specifically, the animal is an animal such as an echinoderm, crustacean, mollusk, fish, amphibian, reptile, Mammals, preferably birds including poultry such as pheasants or chickens or mammals such as humans, pigs, and goats, more preferably mammals, and most preferably humans.

The antimicrobial composition of the present invention may contain carbohydrate, protein, lipid, vitamins and minerals in addition to the herbal extract. The saccharide may be appropriately selected depending on the purpose and use of the saccharide, and examples thereof may include honey, dextrin, sucrose, palatinose, glucose, fructose, starch syrup, sugar alcohol, sorbitol, xylitol, maltitol, But is not limited thereto. The protein may be selected depending on the purpose and use of the protein. Examples of the protein include milk-derived proteins such as casein and whey protein, soybean proteins, animal-derived enzymes such as trypsin and pepsin And hydrolysates by neutrase and alkylase, but are not particularly limited thereto. The lipid may be selected depending on the purpose and use of the lipid. Examples of the lipid include sunflower oil, rapeseed oil, safflower oil, safflower oil, corn oil, etc. containing a first unsaturated fatty acid, a polyunsaturated fatty acid, , A variety of plant-derived fats such as soybean oil, palm oil and palm oil, middle-chain fatty acid, EPA, DHA, soybean-derived phospholipids and milk-derived phospholipids. The above-mentioned vitamins are not particularly limited, and the minerals may be appropriately selected depending on the purpose and use of the minerals. Examples of the vitamins include potassium phosphate, potassium carbonate, potassium chloride, sodium chloride, calcium lactate, calcium gluconate, calcium pantothenate, , Magnesium chloride, ferrous sulfate, sodium hydrogencarbonate, but is not particularly limited thereto. The saccharide, protein, lipid, vitamins, and minerals may be contained in the composition as long as the antimicrobial activity of the composition is maintained. The content of each component is not limited as long as the antimicrobial activity is maintained By weight, preferably 0.1% by weight to 15% by weight, preferably 0.5% by weight to 10% by weight, based on the total composition.

The antimicrobial composition of the present invention can be used for various purposes and applications requiring antimicrobial activity. More specifically, the antimicrobial composition of the present invention is useful as a composition for preventing or treating diseases caused by the pathogenic microorganism infection or for preventing or improving diseases caused by the pathogenic microorganism infection Food composition, animal feed composition, animal feed additive, food, food additive, food preservative, beverage, cosmetic composition, cosmetic additive or detergent.

In this respect, the present invention provides a composition for the prevention or treatment of diseases caused by infection of one or more pathogenic microorganisms selected from the group consisting of pathogenic microorganisms, methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus.

The disease caused by infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus refers to various infectious diseases which can be caused by infection with Staphylococcus aureus. Specifically, Infectious endocarditis, intestinal infections, intestinal infections, intractable pressure ulcers, or wound infections. More preferably, the infective endocarditis is caused by pneumonia, sepsis, bacteremia, infective endocarditis, and enteritis. Lt; / RTI >

The composition for preventing or treating disease caused by infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus according to the present invention may be used pharmacologically according to the formulation and use method in addition to the herbal extract The composition according to the present invention may further comprise a carrier or an excipient capable of additionally containing the composition of the present invention or a disease caused by infection of at least one pathogenic microorganism selected from the group consisting of Staphylococcus aureus or methicillin resistant Staphylococcus aureus and vancomycin resistant Staphylococcus aureus May be administered in combination with other pharmacologically active ingredients to enhance efficacy as a therapeutic agent for < RTI ID = 0.0 > In this case, the content of the herbal extract in the composition for preventing or treating diseases caused by methicillin-resistant Staphylococcus aureus infection may be 0.001 wt% to 99 wt%. When the content of the active ingredient in the composition is less than 0.001% by weight, a large dose may be necessary for effective efficacy, and when it is more than 99% by weight, the efficacy may be constant compared with the usage amount, which may be uneconomical.

The composition for preventing or treating disease caused by infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus according to the method of use and the purpose of use may appropriately control the content of the herbal extract .

The composition for preventing or treating disease caused by infection of one or more pathogenic microorganisms selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus of the present invention may be administered orally or parenterally, However, the dosage form may vary depending on the method of use, and therefore, the dosage form is not limited to those described below.

Examples of such formulations include, but are not limited to, PLASTERS, GRANULES, LOTIONS, POWDERS, SYRUPS, LIQUIDS AND SOLUTIONS, AEROSOLS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, INFUSIONS, TABLETS, INJECTIONS, CAPSULES, and PILLS.

Furthermore, compositions for the prevention or treatment of diseases caused by the infection of one or more pathogenic microorganisms selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus of the present invention can be prepared using a suitable method known in the art Or Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA).

The excipient such as the above pharmacologically acceptable carrier or diluent, preservative, stabilizer, wetting agent, emulsifier, solubilizer, sweetener, colorant, osmotic pressure regulator and antioxidant may be a conventional substance according to the formulation, , Extender, wetting agent, disintegrant or surfactant. Representative diluents or excipients include water, dextrin, calcium carbonate, lactose, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, liquid paratine and saline.

Specifically, in the case of oral administration according to the formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant or the like may be used which is usually used. In the case of parenteral administration, a sterilized aqueous solution, Suspensions, emulsions, freeze-dried preparations, suppositories, etc. may be used. The non-aqueous solutions or suspensions may be, for example, vegetable oils such as propylene glycol, polyethylene glycol, olive oil, injectable esters such as ethyl oleate, and the like. Examples of the suppository include wipesol, macrogol, 61, cacao paper, laurin or glycerogelatin.

In addition, the composition for preventing or treating diseases caused by infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus according to the present invention may be used as a pharmaceutical agent such as physiological saline or organic solvent Can be used in admixture with various acceptable carriers and can be formulated with various excipients such as carbohydrates such as glucose, sucrose or dextran, antioxidants such as ascorbic acid or glutathione, chelating agents chelating agents, low molecular weight proteins or other stabilizers.

An object to be administered of the composition for preventing or treating diseases caused by the infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus of the present invention is an epidermis animal, a crustacean, a mollusk, Amphibians, reptiles, birds, mammals, preferably mammals, more preferably humans.

The dosage or amount of the composition for preventing or treating diseases caused by infection of one or more pathogenic microorganisms selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus of the present invention may be appropriately determined depending on the weight, age, sex, For example, an effective daily dose of 10 mg / kg to 5000 mg / kg, preferably 100 mg / kg, in consideration of the health condition, diet, administration time, administration method, excretion rate and severity of disease. mg / kg to 3000 mg / kg, and may be administered once to several times a day, preferably one to three times a day.

It will be apparent to those skilled in the art that the above dosage may vary depending on various conditions, so that the dose is not limited in any way by the scope of the present invention. The number of administrations can be administered once or several times a day within a desired range, and the administration period is not particularly limited.

In addition, the composition for preventing or treating diseases caused by infection of one or more pathogenic microorganisms selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus of the present invention may be administered orally, And may be ingested on a daily basis.

The composition for preventing or treating diseases caused by infection of at least one pathogenic microorganism selected from the group consisting of methicillin-resistant Staphylococcus aureus and vancomycin-resistant Staphylococcus aureus according to the present invention is characterized by an antimicrobial effect against an excellent methicillin-resistant Staphylococcus aureus It is expected that it can be used for a long period of time since it contains natural plant as an effective ingredient as well as providing an effect of preventing or treating infection.

The present invention also provides a cleaning composition comprising the above-mentioned antimicrobial composition. The cleaning composition may preferably be a detergent for clothes, a dishwashing detergent, a food cleaner or a household appliance cleaner. The antimicrobial composition is a natural ingredient and is not harmful to human body. Therefore, it is more preferably used for the purpose of washing or antibacterial property of various daily necessities including kitchen utensil or food washing.

The detergent may further contain an adversely selected additive depending on the purpose and use of the detergent. The detergent may be mixed with other active ingredients, such as detergents, and additives such as pigments, surfactants and preservatives. The detergent may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the detergent, and a conventional method may be used for packaging for commercialization.

The amount of the antimicrobial composition to be used in the cleaning agent may be suitably used according to the purpose of use, the application form, the application site, the article to be applied and the like, for example, 0.01 to 50 parts by weight based on the total composition, The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

The herbal extract, which is an effective ingredient of the antimicrobial composition of the present invention, has an antibacterial effect against an infectious microorganism, specifically, an antimicrobial effect against an infectious microorganism such as Staphylococcus aureus, Escherichia coli, Clevesiella, Bacillus, Microcarcus, (MRSA) and vancomycin-resistant Staphylococcus aureus (VRSA) as well as natural antimicrobial agents and antibiotic-resistant Staphylococcus aureus And it is considered that the antimicrobial activity against Escherichia coli and pneumoniae bacillus is highly possible to be used as a cleaning composition capable of effectively preventing various diseases. Compositions can be used in a variety of applications, including the food industry, the pharmaceutical industry, and the personal care industry. It can be widely used in industries related to the control of bacteria.

FIG. 1 is a flowchart illustrating a process for producing a herbal extract and a fraction of the present invention, according to an embodiment of the present invention.
FIG. 2 is a photograph showing an antibacterial effect according to an extraction target site and an extraction solvent of a heather extract according to an embodiment of the present invention, wherein D represents an aqueous solution of 70% aqueous methanol solution of A, and A represents an aqueous solution of 70% methanol B is the hexane fraction of 70% aqueous methanolic aqueous solution extract, and C is the ethyl acetate fraction of 70% aqueous methanolic aqueous solution of the herbal flower.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

All experimental results were expressed as mean value repeatedly at least three times, and comparison of each group was determined to be significant in the case where each p <0.05 using ANOVA test.

[ Experimental Example . Heal  Preparation of extract and measurement of antibacterial activity]

Manufacturing example  One. Heal  Preparation of extract

Herring extract was prepared by the method shown in Fig.

First, after purchasing a herring cultivated in Naju area, Jeollanam-do, the herring was washed and washed three times to remove impurities. Then, the flower was divided into flowers and stems, and the flowers, herring roots, The washed herring was completely dried by performing drying for 30 hours. Each of the dried heather flowers, herring roots and herring stems was pulverized to prepare respective dry samples. 5 kg of a 70% methanol aqueous solution was added to 1 kg of each of the above dried flower, root dry or stem dry sample, and the mixture was extracted at 15 DEG C for 72 hours and then concentrated under reduced pressure using a vacuum evaporator. The concentrated aqueous methanol aqueous solution of each herring site was sterilized by using a 400 meah filter cloth as a filter and lyophilized at -20 ° C to prepare a powdery crude extract. The crude extract was dissolved in distilled water to prepare a sample of the herring extract for each herring site. The sample of the herbal extract prepared was stored at -20 ° C. until use.

Further, the above-mentioned 70% aqueous solution of methanol aqueous solution was concentrated under reduced pressure to remove the methanol layer. After the solvent was removed and dried, the obtained extract was dissolved in water, and hexane was mixed at a ratio of 1: 1 on the basis of the volume ratio to the extract dissolved in the water solvent, and the hexane layer was separated twice. The hexane layer was separated, and the hexane layer was separated. The remaining water layer was fractionated twice by mixing ethyl acetate, acetone and butanol at a ratio of 1: 1 on the basis of the volume ratio as described above, and then removing the water layer , And each solvent fraction was prepared.

Experimental Example  1: Measurement of antimicrobial activity

Experimental Example  1-1. Target strain Culture medium  And a sample

The strains used for the determination of the antibacterial activity were separated from the published strains from the depository institutions such as the KTCT or the ATCC, or from the clinical patients of the general hospitals located in Suncheon area, Jeollanam-do, And the strain that was administered to the patient was used. Specifically, the accession numbers of the strains in which the antimicrobial activity is measured are as shown in Table 1 below.

In particular, the antibiotic-resistant strains of the strains which measured the antimicrobial activity were MRSA 693E, MRSA 4-5, MRSA5-3, MRSA S1, MRSA S3, MRSA U4, MRSA P8 and MRSA in the case of methicillin- VRE 3, VRE 4, VRE 5, VRE 6, VRE 82, and VRE 83 were used in the case of vancomycin-resistant Enterococci . In the case of Vancomycin-resistant Enterococci , MRSA2-32 was used for vancomycin resistant Staphylococcus aureus, IMP 100, IMP 102, IMP 123 or IMP 129 and Enterococcus faecalis were used in the case of imipenem resistant resistant bacteria proudcing carbapenemase (IMP), and methicillin Resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enkerocobacter, imipenem-resistant carbapenemase-producing bacterium, Enterococcus faecalis, Klebsiella oxytoca and Klebsiella pneumoniae In addition, Escherichia coli, pneumoniae bacillus, and Salmonella typhimurium strains were used as the isolates of antibiotics resistant bacteria, which were isolated from clinical patients of the general hospitals located in Suncheon area in Jeollanam-do. Respectively.

Each of the test strains was cultured in a medium (muller hinton broth) at a temperature of 37 DEG C and cultured until a McFarland turbidity of 0.5, and then used for an antibacterial test.

Experimental Example  1-2. Measurement of antimicrobial activity

The antimicrobial activity of the preparation example was examined using a paper disk diffusion method (Toama et &lt; RTI ID = 0.0 &gt; al ., 1978). E. coli (KCTC 1923), which is a test strain or a test strain described in Experimental Example 1-1 and Table 1 below, was cultured by the method of Experimental Example 1-1, and then cultured in agar medium (MHB, Muller Hinton agar, Merk Co., Germany) and 0.1 ml of the culture solution was prepared. An extract sample was prepared by dissolving the herbal extract prepared in the above preparation example in a concentration of 10 mg / ml in a 70% aqueous methanol solution, The cells were added to a paper disk (Advantac, USA) having a diameter of 0.8 mm and cultured at 37 ° C. Thereafter, the antimicrobial activity was measured in terms of the presence or absence of growth inhibition and growth inhibition.

First, methicillin-resistant Staphylococcus aureus (MRSA S1), which is a test strain, was plated in order to measure the antimicrobial activity according to the extracted site, that is, a herring flower, a herring roots and a herring stalk. Were measured. As a result of the measurement, antimicrobial effect was not recognized in the case of other extracts of the field except for the flower extracts of Heeraria.

Based on the above results, it has been confirmed that it is appropriate to extract an aqueous methanol solution of a herring flower in order to prepare an antimicrobial composition for an antibiotic-resistant infectious strain.

Further, the fraction of the methanol aqueous solution extract was also measured for antibacterial activity in the same manner as described above. Specifically, the herbal flower 70% ethanol aqueous solution extract prepared in Preparation Example 1 was fractionated using a fraction solvent, and 10 μl of the ethanol aqueous solution extract and its fraction were added to a test strain of methicillin-resistant Staphylococcus aureus MRSA S1) was added to a paper disk (Advantac, USA) having a diameter of 0.8 mm placed on the agar agar medium and cultured at 37 ° C. The antimicrobial activity was measured as the presence or absence of growth inhibition and growth inhibition Respectively. The antimicrobial activity was determined by measuring the size of the inhibitory circle, and the results are shown in FIG.

As shown in FIG. 2, in the case of the extract prepared for the Herb flower, an inhibitory effect was observed and the antimicrobial effect was confirmed. On the other hand, the water fraction or the hexane fraction of the Herb flower extract showed no inhibitory effect, . On the other hand, the ethyl acetate fraction of the 70% methanol aqueous solution prepared from the herbal flowers showed a broader inhibitory effect than the herberry flower extract, and the antibacterial effect, that is, the antimicrobial effect against the methicillin resistant Staphylococcus aureus, It was confirmed to be large.

Based on the above results, it has been confirmed that the extracts using 70% methanol aqueous solution as a extraction solvent of Herring flowers as an extraction target, which is the most effective antimicrobial activity, have antibacterial activity against infectious strains having various antibiotic resistance, Are shown in Table 1 below. In the following Table 1, "+" means that the growth inhibition loop is confirmed and the antimicrobial activity is recognized, and "-" means that the growth inhibition loop is not confirmed and the antimicrobial activity is not recognized.

NO Gram Strain type sensibility One - Alacligenes faecalis ATCC1004 - 2 + Enterococcus faecalis ATCC29212 + 3 + Bacillus subtilis ATCC6633 + 4 + Staphylococcus aureus KCTC1928 + 5 + Micrococcus luteus ATCC9341 + 6 + Mycrobacterium smegmatis ATCC9341 + 7 - Salmonella typhimrium KCTC1925 + 8 - Escherrichia coli KCTC1923 + 9 - Pseudomonas aeruginosa KCTC + 10 + MRSA 693E + 11 + MRSA 4-5 + 12 + MRSA 5-3 + 13 + VRE 82 + 14 + VRE 89 + 15 + VRE 98 + 16 + VRSA (MRSA2-32) + 17 + MRSA S1 + 18 + MRSA S3 + 19 + MRSA U4 + 20 + MRSA P8 + 21 + MRSA B15 + 22 IMP 100 + 23 IMP 102 + 24 IMP 120 + 25 IMP 123 + 26 IMP 129 + 27 + VRE 2 + 28 + VRE 3 + 29 + VRE 4 + 30 + VRE 5 + 31 + VRE 6 + 32 ESBL LMH-B1 + 33 ESBL LMH-P3 + 34 ESBL LMH-S1 + 35 ESBL LMH-U4 + 36 + Enterococcus faecium + 37 - Enterococcus cloacaa + 38 - Klepsiella oxytica - 39 - Klepsiella pneumonia -

As shown in Table 1, the 70% aqueous methanol solution of the present invention was found to have antimicrobial activity against various infectious strains, and in particular, all kinds of antibiotic resistant strains, Staphylococcus aureus, specifically methicillin resistant yellow It has also been found that it has antimicrobial activity against Staphylococcus aureus and vancomycin resistant Staphylococcus aureus, vancomycin resistant enterococci (VRE) and imipenem resistant carbapenemase producing bacteria (IMP). Therefore, it has been confirmed that the aqueous herbal flower alcoholic extract of the present invention has an excellent antimicrobial activity against antibiotic resistant strains including Staphylococcus aureus having antibiotic resistance. From these facts, it can be said that the treatment, improvement or improvement of infectious diseases caused by Staphylococcus aureus It was evaluated that it could be useful for prevention.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (8)

Antibacterial for hieori (Corylopsis coreana) flowers 70% methanol aqueous solution extract of ethyl acetate fraction of methicillin-resistant Staphylococcus aureus (Methicillin-Resistant Staphylococcus aureus) and vancomycin resistant Staphylococcus aureus (Vancomycin-Resistant Staphylococcus aureus), including the active ingredient of the Lt; / RTI &gt; delete The method according to claim 1,
The antimicrobial composition may be selected from the group consisting of methicillin resistant Staphylococcus aureus, vancomycin resistant Staphylococcus aureus, E. coli , Bacillus sp., Micrococcus sp., Staphylococcus sp. , Enterococcus bacteria (Enterococcus sp.), Salmonella (Salmonella sp.), keulrep when Ella (Klebsiella sp.) and Pseudomonas bacteria, characterized in that the antimicrobial composition having an antimicrobial activity on (Pseudomonas. sp). delete delete delete A cleaning composition comprising the antimicrobial composition of claim 1 as an active ingredient. 8. The method of claim 7,
Wherein the cleaning composition is a detergent for clothes, a dishwashing detergent, a food cleaner or a household appliance cleaner.

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