KR101713475B1 - Injectable tissue volume replacement material comprising cartilage - Google Patents

Abstract

The present invention relates to a bulk replacement material for injection transplantation, and more particularly, to a reconstructed volume replacement material containing a cartilage for skeletal tissue defect repair and skeletal tissue bulking as well as skin repair and cosmetic improvement of skin due to trauma, Lt; RTI ID = 0.0 > transplantation < / RTI > The cartilage-containing volume replacement material containing cartilage according to the present invention can be manufactured by a relatively simple treatment process, a simple procedure using a syringe can be applied, scars are scarcely left after the procedure, inconvenience, Less swelling and a shorter recovery period.

Description

Injectable tissue volume replacement material comprising cartilage < RTI ID = 0.0 >

The present invention relates to a body volume replacement material for injection implantation including a cartilage, and more particularly, to a method for repairing damage or increasing the volume of soft tissues such as restoration of skin tissue damage due to trauma or the like, And to cartilage-containing injectable transplantable body bulk replacements that can be applied for the repair of skeletal tissue defects and the enhancement of skeletal tissues.

Methods of increasing body volume using conventional injections have been used to increase the volume of soft tissues such as skin by using synthetic materials such as autologous adipose tissue, homogenous or heterogeneous collagen, PLGA (poly lactic coglycolic acid) particles and gore-tex Soft tissue augmentation methods have been used predominantly.

These methods can increase the volume of soft tissues when the soft tissues and skeletal tissues are simultaneously defected. However, there are limitations in restoring or increasing the volume of the skeletal tissues, and it is not permanent, .

In Korean Patent Laid-Open Nos. 10-2002-0084056 and 10-2004-0058818, chondroblasts, osteoblasts, and lipid precursor cells are collected and chitosan sponge powder having a solid sponge structure, polyethylene, polytetrafluoroethylene (PTFE) Particles, PLGA (poly lactic coglycolic acid), etc., as a support structure, and then injected. These methods can increase the amount of the graft material, but they can be cultured by culturing the cells The complication of culturing process, the cost of expensive treatment, the prolongation of the operation period, and the inconvenience of patients suffering from side effects due to foreign substances due to the use of support structures made of foreign substances, There was a problem accompanying it.

KR 10-2002-0084056 A KR 10-2004-0058818 A KR 10-2004-0048382 A

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and it is an object of the present invention to provide a cartilage which is separated from a human body, And to provide a body bulk substitute for injection grafts containing cartilage, which is capable of recovering bone defects and enhancing volume.

DISCLOSURE OF THE INVENTION In order to solve the above problems, the inventor of the present invention has conducted intensive research and found that, as a result, the cartilage is pulverized to a predetermined size and then mixed with one or more kinds selected from physiological saline and body fluid, As a result, it was surprisingly found that the bulk substitution and the enhancing effect were maintained without side effects for a long period of time, thereby completing the present invention.

Prior to the present invention, it has been attempted to apply soft tissue such as adipose tissue as an injection, but no attempt has been made to apply the cartilage itself directly as an injection.

Therefore, according to the present invention, a simple procedure such as injecting a body-volume replacement material containing cartilage in a state of preserving the structure of the cartilage and the fibrous material of the cartilage as it is, does not show side effects such as scarring It has been shown for the first time that damage to soft tissue or skeletal defect can be effectively treated.

In a preferred embodiment, the present invention provides cartilage separated from the body, comprising cartilage comminuted to a size of 0.5 to 1.5 mm.

The cartilage is preferably softened.

The cartilage is preferably at least one selected from the group consisting of ear cartilage, septum cartilage, and rat cartilage.

The volume substitute also includes at least one selected from physiological saline and body fluids.

It is preferable that a mixing volume ratio of at least one selected from the cartilage, physiological saline, and body fluid is 3: 7 to 7: 3.

The cartilage-containing volume replacement material for cartography according to the present invention can be manufactured by a comparatively simple treatment process, and can be applied not only to soft tissue but also to restore and increase volume of skeletal tissue. Also, a simple procedure using a syringe can be applied There is little scar left after the procedure, the patient feels discomfort after the procedure, the swelling is small, and the recovery period is shortened.

Figure 1 shows cartilage separated from the body.
Fig. 2 shows a state in which cartilage is crushed.
Fig. 3 shows a state in which the pulverized cartilage is filled into a syringe.
Fig. 4 shows a state in which the cartilage mixed with the physiological saline is filled in the syringe.
FIG. 5 shows a photomicrograph of a transplanted cartilage cell one year after transplantation of a volume replacement material for injection injection of the present invention into a patient. The part dyed in purple represents living chondrocytes.

Hereinafter, the present invention will be described in detail.

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bulk replacement material capable of being injected and injected for injection (hereinafter, referred to as 'injection implantation') for the recovery and volume enhancement of soft tissues and skeletal tissues of a human body, The present invention relates to a body-size substitute manufactured by a simple treatment process of extracted cartilage itself.

Since the body volume replacement material of the present invention can be injected into a region to be implanted by filling the syringe and injected by injection, unlike the conventional implantation method through incision, there is almost no scar It can be applied to the volume of the remaining body or skeletal replacement procedure.

According to the present invention, there is provided a bulk replacement material for injectable grafts, which comprises cartilage isolated from the body, and comprising cartilage ground to a size of 0.5 to 1.5 mm.

The term " body " refers to the body of a mammal, preferably a human body, and specifically refers to human soft tissue and skeletal tissue.

The term 'cartilage' includes not only the cartilage (autologous cartilage) of the patient himself / herself to be treated but also cartilage of the other, and may be selected from the group consisting of the ear cartilage, the septum cartilage and the rheumatoid cartilage, The donor cartilage may be determined depending on the patient's condition. Preferably, autologous bone is used.

The term 'isolated cartilage' refers to a cartilage already separated from the body by surgical operation, and does not mean cartilage attached to the body.

The cartilage should be milled to a size suitable for operation with a syringe, such as a cannula, and the size of the mill may vary depending on the needle size of the syringe used. The volume replacement material for injectable implants of the present invention is preferably injected using, for example, a cannula having a needle outer diameter of 1.8-2.5 mm of the syringe. If the needle diameter of the syringe is too small, It may be difficult to inject the substitute material, and if the needle diameter of the syringe is too large, the injection needle hole remaining in the body during the procedure may become large and the scar may remain, which is undesirable. When the bulky substitute of the present invention is injected using, for example, a cannula having a needle outer diameter of 1.8-2.5 mm of the needle of a syringe, the cartilage is crushed to a size of 0.5 to 1.5 mm in width, . The grinding may be performed using a surgical knife, a cutter, or the like. The pulverization is preferably carried out along the texture of the cartilage so that the possible cartilage tissue is not damaged. When the size is less than 0.5 mm, various kinds of fiber structures may be broken in the matrix quality of the cartilage tissue structure, and the cartilage cells in the cartilage lobes may be destroyed to receive the surrounding nutrients and oxygen, And when it exceeds 1.5 mm, there is a problem in transplanting into a patient with a syringe.

It is preferable that the cartilage undergoes a softening process before or after being crushed. The softening process may be performed by a conventional method using a medical cartilage softening device (e.g., mallet) or the like.

The pulverized cartilage is mixed with at least one selected from physiological saline and body fluids. Herein, the " body fluid " is a liquid substance obtained by separating from the body, and includes a liquid substance But are not limited to, serum, platelet rich plasma (PRP), and stromal vascular fraction (SVF), for example. The mixing ratio of the cartilage, the physiological saline solution and the body fluid may be adjusted in consideration of the needle size of the syringe used and the easiness of injection at the time of operation. For example, one kind selected from crushed cartilage: physiological saline and body fluids Is preferably in the range of 3: 7 to 7: 3, more preferably in the range of 4: 6 to 6: 4. When the content of cartilage is less than the above range, the amount of physiological saline or the like is relatively large, swelling at the time of injection is increased, and it is difficult to precisely confirm the volume replacement effect at the time of the procedure, Is more than the above range, there is a problem that it may be difficult to inject.

The body cavity substitute for injectable transplantation of the present invention thus prepared can be injected directly into a transplantation site through a syringe or the like.

Hereinafter, the present invention will be described in more detail with reference to specific examples.

[ Example ]

1. Manufacture of body bulk substitutes

Ear cartilage which can be easily obtained from the body was used as donor cartilage (Fig. 1). Since the cartilage tissue structure has various kinds of fiber structures in the matrix and the cartilage cells are contained in the cartilage lobe, the fibrous structure and cartilage cells are basically not damaged, and the contact area with the transplantation tissue is widened, The cartilage was first cut to length of 1 mm in the longitudinal direction. The soft tissue was slightly different depending on the type of donor cartilage, but the soft tissue was retained in the direction of preserving the elastic fibers and other fibers contained in the cartilage. (Fig. 2). Thereafter, the crushed cartilage was filled in a 1 cc syringe (Fig. 3), and physiological saline was mixed (Fig. 4), and the crushed cartilage: physiological saline weight ratio was 4: 6.

2. Skin transplantation

Using a 2-0 cannula (needle outer diameter: 2.0 mm) used for injecting fat, a hole of about 2 mm was made in a region to be injected using the tip of a syringe, A substitute was injected.

3. Follow-up after transplantation

When the nutrients are not diffused and supplied from the surrounding tissue to the injected cartilage, the cartilage cells die. However, as shown in FIG. 5, when the cartilage cells were treated using the volume replacement material of the present invention, the cartilage cells of the implanted cartilage were alive .

When the transplanted cartilage cells die, the cartilage tissue is absorbed by the surrounding tissues and the volume is turned off. As a result, the volume replacement effect decreases with time. As a result of the operation using the skin transplanting composition of the present invention, As shown in FIG. 5, even when the cartilage is stored as it is and the cartilage is piled up in several layers, nutrients can easily diffuse from the surrounding tissues. Therefore, even after one year from the injection, It was confirmed that no absorption or off phenomenon occurred.

Claims (4)

(1) a softening step for softening the cartilage separated from the body;
(2) a grinding step of cutting and crushing cartilage of softened cartilage in a size of 0.5 to 1.5 mm in width, height, and height along the cartilage texture so as not to damage cartilage tissue;
(3) a step of mixing cartilage cut to a predetermined size through the crushing step, at least one selected from physiological saline and body fluid at a volume ratio of 4: 6 to 7: 3;
≪ / RTI > The method of claim 1, (1) a step of grinding cartilage separated from the body by cutting and crushing the cartilage to a size of 0.5 to 1.5 mm in width, height and height along the cartilage texture so as not to damage cartilage tissue;
(2) a softening step of subjecting the cartilage cut and ground to a predetermined size through the grinding step;
(3) a step of mixing at least one selected from the group consisting of physiological saline and body fluids at a volume ratio of 4: 6 to 7: 3 by cutting, crushing and softening the cartilage to a predetermined size through the grinding and softening step;
≪ / RTI > The method of claim 1, 3. The method according to claim 1 or 2, wherein the cartilage is selected from the group consisting of ear cartilage, septum cartilage, and rat cartilage. 3. The method of claim 1 or 2, wherein the body fluid is selected from serum, platelet-rich plasma (PRP), and adipose stem cell fraction (SVF).

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