KR101706284B1 - Fumigation type compositions having antimicrobial and antifungal activity and Fumigation apparatus comprising the same - Google Patents

Abstract

The present invention provides fumigant antimicrobial compositions including time oil, isothymol and chloroxylenol as fumigation and antibacterial components and fumigants containing the same. The composition of the present invention exhibits a wide range of antimicrobial effects against Gram-positive bacteria, Gram-negative bacteria and fungi through fumigation of time oil, isothymol and chloroxylenol. Therefore, the fumigant provided with the composition of the present invention can be sterilized Disinfection and so on.

Description

TECHNICAL FIELD The present invention relates to a fumigation type antimicrobial composition and a fumigation method including the same,

The present invention relates to fumigant antimicrobial compositions comprising time-oil, isothymol and chloroxylenol, and fumigants containing the same.

The fumigation (fumigation disinfection device) is used for interior disinfection of a building and disinfection of a vehicle interior. The fumigant is an antimicrobial effect that fumigates the fumigant that has the antimicrobial activity that the high heat generated by the exothermic reaction of water and calcium oxide is equipped in the fumigant.

Since fumigant is evaporated into the air, effective ingredients such as antimicrobial agents are more effective in airtight room, and fumigant medicines work in all corners of the room, which can kill fungi and insect pests that are not reachable by human hands .

Thus, since the fumigant vaporizes the fumigant to the air, it achieves the antimicrobial effect, so that the fumigant applicable to the fumigant must be able to be released to the air by heat, Should exhibit antibacterial activity.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

Korean Patent Publication No. 10-2014-0104011 (2014.08.27)

The present inventors have made efforts to develop an antimicrobial material for fumigation that exhibits excellent antimicrobial activity against bacteria and fungi even when they are diffused (fumigated) into the air in a gaseous state. As a result, it has been confirmed that a wide range of antibacterial effects against bacteria and fungi can be obtained by fuming time oil, which shows only a slight antimicrobial activity at fumigation, with isothymol and chloroxylenol, thereby completing the present invention .

Accordingly, it is an object of the present invention to provide a fuming antibacterial composition.

Another object of the present invention is to provide fumigant.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a fuming antibacterial composition comprising Thyme Oil, isothymol and chloroxylenol (PCMX).

The present inventors have made efforts to develop an antimicrobial material for fumigation that exhibits excellent antimicrobial activity against bacteria and fungi even when they are diffused (fumigated) into the air in a gaseous state. As a result, it was confirmed that a wide range of antimicrobial effects against bacteria and fungi could be obtained by fuming time oil, which shows only a slight antibacterial activity at fumigation, with isothymol and chloroxylenol.

As used herein, the term "fuming antibacterial composition" refers to a composition comprising a component that is capable of being released into the air by heat to inhibit the growth of bacteria and fungi, or exhibit a bactericidal action.

According to the present invention, the time oil, isothymol and chloroxylenol are included in the composition as fumigation and antibacterial components. The term "fumigant component" means a component that is heated to the air by heat, and the term "antibacterial component " means a component that inhibits the growth of bacteria and fungi or exhibits a bactericidal action.

According to one embodiment of the present invention, the time oil is contained in an amount of 0.8-2.0 wt% based on the total weight of the composition.

In one specific example, the time oil is comprised in the range of 0.8-1.9 wt.%, 0.8-1.8 wt.%, 0.8-1.7 wt.%, 0.8-1.6 wt.% Or 0.8-1.5 wt.%, 0.9-1.9 wt.%, 0.9-1.7 wt.%, 0.9-1.6 wt.% Or 0.9-1.5 wt.%, In another specific example 1-1.9 wt.%, 1-1.8 wt. %, 1-1.7% by weight, 1-1.6% by weight or 1-1.5% by weight.

According to one embodiment of the present invention, the weight ratio of isothiomol: time oil: chloroxylenol in the composition is 1: 0.05-0.14: 0.8-1.1. As described in the following examples, the fumigant cans (fumigant cans B and F) equipped with the composition containing the three components at the above-mentioned weight ratio showed a perfect antibacterial activity against all bacteria and fungi, unlike the other fumigants See Tables 8 and 9).

In one particular example, the weight ratio of isotimol: time oil: chloroxylenol is 1: 0.05-0.13: 0.8-1.1, 1: 0.05-0.12: 0.8-1.1, 1: 0.05-0.11: 0.8-1.1 or 1: 0.05-0.10: 0.8-1.1, in other specific examples 1: 0.06-0.13: 0.8-1.1, 1: 0.06-0.12: 0.8-1.1, 1: 0.06-0.11: 0.8-1.1, 1: 0.06-0.10 : 0.8-1.1 or 1: 0.065-0.10: 0.8-1.1, and in another specific example 1: 0.05-0.13: 0.9-1, 1: 0.05-0.12: 0.9-1, 1: 0.05-0.11: 0.9-1 Or 1: 0.05-0.10: 0.9-1 and in another specific example 1: 0.06-0.13: 0.9-1, 1: 0.06-0.12: 0.9-1, 1: 0.06-0.11: 0.9-1, 1: 0.06 -0.10: 0.9-1 or 1: 0.065-0.10: 0.9-1.

According to one embodiment of the present invention, the composition of the present invention further comprises a wax. The wax is impregnated with fuming and antibacterial components (time oil, isothymol and chloroxylenol), and as the wax is melted by heating, the fumigant and antimicrobial components diffuse into the air in a gaseous state.

In the present invention, a solid at room temperature or a wax which is melted by heating to become a liquid phase can be used without limitation. Such waxes include natural waxes including vegetable waxes, animal waxes, mineral waxes, and petroleum waxes, and synthetic waxes.

Examples of the vegetable wax include carnauba wax, Kendilla wax, rice wax, wood wax, bayberry wax and jojoba oil; Examples of the animal wax include beeswax, lanolin, beeswax, wool wax, beeswax, shellac wax and superhemeteceti wax; Examples of the mineral wax include montan wax, ozokerite wax and ceresin wax; Examples of the petroleum wax include paraffin wax, microcrystalline wax, petrolatum and liquid paraffin; Examples of the synthetic wax include synthetic hydrocarbon waxes such as Fischer-Tropsch wax and polyethylene wax, modified waxes such as montan wax derivatives, paraffin wax derivatives and microcrystalline wax derivatives, hydrogenated waxes such as hydrogenated waxes Castor oil and derivatives thereof) and fatty acid amide ester ketone waxes (e.g., stearic acid amide, non-hydantoic acid amide, and chlorinated hydrocarbons).

The fuming antimicrobial composition of the present invention may further include a foaming agent, an insecticide component, an insect repellent component, a deodorant component, an aroma, or a combination thereof in addition to the above-mentioned components. These components may be provided in the form impregnated with the wax.

According to one embodiment of the present invention, the composition of the present invention exhibits antibacterial activity.

Examples of the bacterium that can exhibit the antibacterial activity of the composition of the present invention include Staphylococcus aureus , Streptococcus pneumoniae , Bacillus cereus , Escherichia coli , . S. aureus is widely known to be widely distributed in the living environment, and S. aureus and B. cereus induce food poisoning when food is infected with these bacteria, It is known to cause enteritis by toxins.

In particular, B. cereus is known to secrete Nhe (non-hemolytic enterotoxin), Hbl (hemolytic enterotoxin) and CytK (cytotoxin K), which are toxins that cause vomiting toxins such as cereulide or diarrhea and enteritis as soil and airborne microorganisms have. As described in the examples below, when the oil alone, including time oil, was applied to the fumed can, the antimicrobial effect against B. cereus could not be obtained (see Table 6). Combination of time oil with isothymol and PCMX, (See Tables 8 and 9). ≪ tb >< TABLE >

According to one embodiment of the present invention, the composition of the present invention exhibits antifungal activity.

The fungus in which the composition of the present invention can exhibit antifungal activity is, for example, Candida albicans .

According to another aspect of the present invention, there is provided a fumigant comprising the fuming antibacterial composition of the present invention.

The fuming antibacterial composition of the present invention is applicable to various fumigants. The fumigant is an apparatus that performs an antibacterial action by heating fumigant type antimicrobial composition through a heating means and fumigating the fumigant and antibacterial component into the air. The fumigant of the present invention can be prepared through a method of providing the fumigant antimicrobial composition of the present invention to various fumigants known in the art.

As the heating means, known heating means such as hydrothermal heating means, air oxidizing heat generating means and electric heating means can be used.

According to one embodiment of the present invention, a hydrothermal heating method is applied to the fumigant of the present invention. As the hydrothermal substance, substances such as calcium oxide, magnesium chloride, aluminum chloride, calcium chloride and iron chloride, which initiate an exothermic reaction by adding water, are used.

According to one embodiment of the present invention, the fumigant of the present invention is fumigant for indoor disinfection of a vehicle.

The features and advantages of the present invention are summarized as follows:

(I) The present invention provides fumigant antimicrobial compositions including time oil, isothymol and chloroxylenol as fumigation and antibacterial components, and fumigants containing the same.

(Ii) Since the composition of the present invention exhibits a wide range of antimicrobial effects against Gram-positive bacteria, Gram-negative bacteria and fungi through fumigation of time oil, isothymol and chloroxylenol, the fumigant provided with the composition of the present invention can be sterilized And disinfecting the interior of the building.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Materials and Experiments

Published species

Staphylococcus aureus (ATCC 25923), Streptococcus pneumoniae (ATCC 49619), Bacillus cereus (ATCC 27438) and Escherichia coli (ATCC 25922) were used as fungi and Candida albicans (ATCC 10231) was used as fungi. The strains used in this experiment were purchased from the Korean Microorganism Conservation Center (KFCC, Hongje-dong, Seoul).

Paper disk diffusion method

The antimicrobial activity of each of the desired strains including pathogenic bacteria was measured using the paper disk method. Each strain was diluted in 0.85% physiological saline solution and the turbidity was adjusted to a suitable turbidity using a turbidimeter. The turbidity-matched bacteria were inoculated onto a muller hinton agar (MHA), and the cells were diluted three times in a sterilized paper disk (Advantec filter paper, Toyo Roshi Kaisha Ltd., Japan) having a diameter of 10 mm After 15 μl of 0.85% physiological saline was adsorbed to the sample (stock solution, 70%, 50%) and the negative control, the solvent was evaporated, and the mixture was adhered to the surface of the plate medium at 37 ° C. for 24 hours. Clear zone was measured.

Minimum inhibitory concentration and minimum killing concentration

The minimum inhibitory concentration (MIC) assay was performed using a broth dilution method (Mann, CM and Markham A, JL Journal of Applied Microbiology . 1998; 84: 538-44). The antimicrobial material was diluted with 99% alcohol and diluted to a concentration of 0-20 ㎍ / ml. For each strain, the concentration was adjusted by using a turbidimeter and then diluted 100 times to be used in this experiment. 500 μl of time oil was continuously diluted in 500 μl of MHB (muller hinton broth, MHB) in a sterilized Kahn tube, and then 500 μl of a bacterial dilution was sequentially added and cultured at 37 ° C. for 18-22 hours. Thereafter, turbidity was visually observed, and compared with the negative and positive control groups, the minimum concentration at which no bacterial growth was detected on the growth curve of the microorganism was set as MIC.

The minimum bactericidal concentration (MBC) was determined by taking 100 μl of the culture samples for each time zone for determination of MIC, plated on the basal selection medium of each microorganism, culturing for 24-48 hours at 37 ° C, When the number was observed, the minimum concentration to completely kill (99%) the target bacteria was set to MBC. All experiments performed three independent experiments and synthesized the results. All experiments with minimum inhibitory concentration (MIC) and minimum killing concentration (MBC) of microorganisms including disk diffusion method were performed in accordance with CLSI (Clinical & Laboratory Standards Institutes) guidelines.

Manufacture of fumigation cans

After the water bath was warmed, the wax was quantitatively weighed and sufficiently dissolved at a temperature of 70 ° C. Chloroxylenol and isothymol were added to the mixer in a fixed amount, followed by stirring for 10 minutes while continuously dissolving the powders so as not to aggregate. After confirming that the wax was sufficiently melted, the incense and time oil were added and mixed uniformly.

A can filled with calcium oxide at the lower end was filled with the wax formulation in a quantitative manner, cooled, and then filled with the mixed powder. A sticker was attached to the top of the can, and two holes were drilled on the top. The can was put into the PP container, and the upper surface was sealed to prepare the fumen can.

Indoor fumigation cans booth test

The McFarland turbidimetric method was used to adjust the number of bacteria to be inoculated to a certain number. To establish the McFarland standards, the reference scale was fitted using methylene blue and sterile saline. In the case of Gram-positive bacteria, 0.5, and 1.5 × 10 ⁸ CFU / ㎖ of bacteria were dissolved in physiological saline and inoculated into BAP medium with sterilized cotton swabs. Gram-negative bacteria were obtained from the same method using McFarland No. 1. 1.0 (3.0 × 10 ⁸ CFU / ml) and then inoculated on MAC medium. 2.0 (6.0 X 10 < ⁸ > CFU / ml) and a certain number of bacteria were inoculated into SDA. After adjusting the number of bacteria through the McFarland method, the medium was inoculated on a selective medium, and then the medium was placed in an acrylic box of a certain size while the lid was opened.

Test group cans (Fumigation cans A to F) for blowing 1 g of isothymol were put into an acrylic box, and the air speed was operated so that fumigation could spread evenly inside the box. After contact with the test material for 10 minutes after the start of fumigation, BAP medium and MAC medium were incubated at 37 ° C for 24 hours. SDA, a fungal laboratory medium, was cultivated at 28 ° C for 48 hours. In order to prevent the test substances from being counted, the test was conducted after all the tape around the acrylic box lid was covered during the experiment. After the incubation, the sterilization ability of the internal fuming cans was evaluated by comparing the media of the experimental group. In the same manner, the fungicidal ability of the fumigant containing only oil as an antibacterial component was evaluated.

Experiment result

Disk diffusion method result

The time oil was diluted with 100% ethanol from the undiluted solution to make 70% and 50%, and then subjected to disk diffusion method to measure the clear zone. As a result, in the case of the time oil stock solution, a clear zone was maximally formed in all Gram-positive bacteria, Gram-negative bacteria and fungi ('max' means 35 mm or more in diameter). Similar results were obtained with the 70% and 50% time oils (Table 1 to 3).

Minimal inhibitory concentration (MIC) measurement results

The minimum inhibitory concentration was 0.0625 ㎍ / ㎖ for S. aureus , E. coli and C. albicans , but no significant effect was observed for B. cereus .

The results of MBC measurement

S, aureus was inhibited at 0.1250 ㎍ / ㎖, E. coli was inhibited at 0.0625 ㎍ / ㎖, and C. albicans was inhibited at 0.0625 ㎍ / ㎖, . All of the bacteria except B. cereus , which is frequently observed under normal environment, showed antibacterial effect (Table 5).

Indoor Fumigation Cans Booth Test Results

First, the fumigation cans were filled with antimicrobial oil (time oil, tea tree oil, or Aki Helper F-55) as an antimicrobial agent.

As a result, as shown in Table 6, an antibacterial effect could not be obtained by fumigating oil alone.

Next, indoor fumigation can booth tests were conducted with fumigation cans containing isothymol and PCMX as well as time oil as an antibacterial component. The test was carried out by varying the content of time oil (0.3, 1.0, 1.5, 3.0, 18.0, 20.0% by weight) as shown in Table 7 (unit in Table 7 is wt%). The experimental results are shown in Table 8.

As shown in Table 8, the fumigant can A containing 0.3% by weight time oil did not inhibit the fungus, but the fumigant can F containing 1.0% time oil and the fumigant can containing 1.5% B inhibited all the species. In the fumigants F and B, haemolysis was distinctly observed in gram-positive bacteria ( S. aureus , S. pneumonia ). In the case of fumigant can C containing 3.0 wt% time oil, no inhibitory effect was exhibited in B. cereus as in the case of disk diffusion method and MIC / MBC. Fumigation cans D containing 18% by weight time oil showed antimicrobial effects, but showed less antimicrobial effect than fumigation cans B and F containing less time oil, and the most abundant 20% Was not able to show antimicrobial effect against all the species. The results of the second experiment were the same as those of the first (Table 9).

Finally, as shown in Table 10 below, the room fumigation can booth test was conducted with fumigant can not contain time oil (the unit in Table 10 is wt%). The experimental results are shown in Table 11.

As shown in Table 11, fumigation cans not containing time oil exhibited a slight antibacterial effect (Identification substance A) or showed almost no antimicrobial effect (Identification substance B).

As described above, the time oil exhibiting excellent antimicrobial activity in the disk diffusion method and the minimum inhibitory concentration / minimum killing concentration test showed almost no antibacterial activity at fumigation (Table 6). In addition to isothiomol and PCMX, Showed excellent antimicrobial activity against the experimental strains (Tables 8 and 9) and showed only slight antibacterial effects even when isothymol and PCMX were fumed without time oil (Table 11).

The results of the above experiments show that when applied to fumigation, time oil is used in combination with isothymol and PCMX to obtain a wide range of antimicrobial effects against gram-positive bacteria, gram-negative bacteria and fungi through fumigation of these components.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

Time oil, isothymol and chloroxylenol, wherein the time oil is contained in an amount of 0.8-2.0 wt% based on the total weight of the composition.
delete The composition of claim 1, wherein the weight ratio of isothiomol to time-oil: chloroxylenol in the composition is 1: 0.05-0.14: 0.8-1.1. The composition of claim 1, wherein the composition further comprises a wax, wherein the wax is impregnated with time oil, isothymol, and chloroxylenol, the time oil, isothymol, and chloroxylenol ≪ / RTI > The composition of claim 1, wherein the composition is selected from the group consisting of Staphylococcus aureus , Streptococcus pneumoniae , Bacillus cereus , Escherichia coli , and combinations thereof. Wherein the composition exhibits bacterial activity. The composition of claim 1, wherein the composition exhibits an antifungal activity against Candida albicans . 7. A fumigation comprising the fuming antimicrobial composition of any one of claims 1 and 6 to 6.