KR101700618B1 - Method for discriminating rice resistance to phosphoric acid deficient stress - Google Patents
Method for discriminating rice resistance to phosphoric acid deficient stress Download PDFInfo
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Abstract
Description
The present invention relates to a method for determining stress tolerance of phosphorus deficiency in rice using a rice straw as a raw material.
Rice is one of the world's four largest food crops and is a stock of Asian people including Korea. Many researchers are trying to improve economic efficiency and yield through various breeding methods such as cross breeding, molecular breeding and transformation methods .
Due to the rapid increase in population, food shortage is becoming a big problem all over the world, and it is required to cultivate multi-sex food crops to overcome this problem. In order to cultivate multiple food crops, it is necessary to supply sufficient nutrients to the crops. One of the main ingredients of fertilizer is phosphoric acid. Phosphoric acid is one of the essential nutrients needed by plants and plays an important role in all metabolism processes occurring in plant cells such as energy transfer, signal transduction, regulation of enzyme activity, biosynthesis of metabolites, photosynthesis and respiration. It is also an important component of phospholipids and nucleic acids, and is the nutrient element most needed by plants along with nitrogen.
Phosphoric acid is an important ingredient in the growth and development of crops, and plants can not synthesize, so absorption through the roots is absolutely necessary. However, most of the phosphoric acid present in the soil is insoluble organic phosphoric acid or phosphoric acid which is combined with iron, calcium, etc., and the available phosphoric acid form that plants can absorb is insufficient. Thus, the fertilizer treated to increase the effective phosphoric acid concentration of the soil required for crop growth causes the increase in agricultural production costs. In addition, fertilizers that have not been absorbed due to excessive use of fertilizer are accumulated in the soil and acidify the soil, adversely affecting the growth of crops, causing groundwater pollution and eutrophication of surface water. The pollution of the agricultural environment not only leads to quantitative and qualitative deterioration in the production of agricultural products, but also pollutes the living environment and threatens the public health. Phosphorus as a raw material of phosphoric acid chemical fertilizer is a mineral with limited reserves and is expected to be depleted in the next 50 to 100 years. Therefore, in order to develop a crop that can reduce application of phosphate fertilizer, Development of smooth crops is necessary.
Under the conditions of phosphorus deficiency, the plant promotes root growth and root hair formation to broaden the surface area, and induces the expression of genes involved in the biosynthesis and secretion of highly phosphorylated phosphoric acid carriers, dephosphorylated enzymes and organic acids, , And it has been reported to promote phosphate availability. Thus, by analyzing the function of rice-derived R2R3-MYB transcriptional regulatory gene (OsMYB4P) expressed in phosphate-deficient conditions and using the gene to enhance the phosphoric acid uptake efficiency of plants, a plant capable of adapting to phosphate- Korean Patent Publication No. 2015-0024574. Korean Patent No. 0640284 discloses a method for producing a high-affinity phosphate carrier gene, a promoter of the gene, and a method for producing a plant transformed using the gene, which is always expressed in all tissues of rice regardless of the change in the phosphoric acid concentration Korean Patent No. 0770203 discloses a method of increasing the absorption of phosphoric acid by a plant using a gene of a tobacco-derived phosphoric acid transporter, and a transgenic plant in which the rate of absorption of phosphoric acid is increased by the same method. However, no method has been disclosed to select a rice strain resistant to phosphorus deficiency, which is capable of securing growth even in a phosphate-deficient environment.
On the other hand, it is known that the cultivation of rice in the soil lacking phosphoric acid significantly decreases the yield and does not affect the plant height and the liver. In addition, it is known that rice is the most important factor for the decrease of yield due to phosphate deficiency, and it is well known that rice is a representative trait of rice which is easy to grow even in lack of soil phosphate. Generally, in order to ascertain the yield of rice according to the soil phosphate content, it is necessary to investigate the growth of the rice after the rice paddy and the highest freezing period. Therefore, in order to select genotypes with uniform growth in phosphate-deficient soil, a large amount of samples must be sown and examined for growth, so labor force and wide packing must be secured. In addition, it takes about 90 days from sowing to the highest freezing stage to maintain the growth up to the highest freezing point.
Under these circumstances, the inventors of the present invention have made intensive researches to develop a method for selecting a liver in a small area and in a short time of a phosphorus-deficient stress tolerance rice which can secure growth even in a phosphorus-deficient environment. As a result, The weight of the rice seedlings grown in the weaning box or pavement containing the seedlings was measured. The heavier the weights of the rice seedlings were, the heavier the mature stage of the rice was, and the more the rice yield was increased. Thus, it was confirmed that the phosphorus-deficient stress tolerance rice paddy can be easily selected and the degree of phosphorus deficiency stress tolerance can be determined by measuring the rice paddy rice grown in the soil without phosphoric acid. Completed.
It is an object of the present invention to provide a method for determining phosphorylation stress resistance of rice.
In order to accomplish the above object, the present invention provides, as one embodiment, (a) a method for growing rice in a phosphate-deficient soil to a rice field stage; (b) measuring the height of the rice paddy field; And (c) determining that resistance to phosphorus deficiency stress is higher as the weight of the measured rice straw is higher .
In one embodiment of the present invention, the rice genetic resources required for selection were prepared in the best manner. In the untransplantation treatments, in which phosphorus-free yellow soil and phosphorus-free nitrogen and potassium were applied in a hair growth box or package, (R 2 = 0.76) were found to be significantly correlated with the growth rate of the rice seedlings and the ripening period. Further, the correlation of the construction of the rice germplasm resulting oil feat rice after irradiation the transfer of the 20 points of analyzing the correlation between of the oil feat construction grown in the unmanned acid treatment and rice cane confirmed the high (R 2 = 0.79).
Accordingly, the present invention relates to a method for producing a phosphate-deficient stress tolerant rice, which is capable of securing stable growth even in a phosphate-deficient soil, without growing the rice to a mature rice plant, And it was confirmed that phosphorus deficiency stress tolerance of rice could be determined.
The method for determining resistance to stress deficiency in rice of the present invention comprises the steps of: (a) growing rice in a phosphate-deficient soil to a stool stage.
The term "deficiency" means a deficiency in the absence or inadequacy of a particular element, and a nutritional disorder caused thereby is called a deficiency. The term "deficiency of phosphorus"
The term "phosphorus-deficient soil" means a soil with no or little phosphoric acid. Specifically, it means soil containing all of other elements in the soil but lacking or lacking phosphoric acid. Specifically, , But it means soil lacking or lacking phosphoric acid. Generally, the content of phosphoric acid contained in the conventional soil (normal soil) necessary for the stable growth of rice is 100 to 150 ppm, and the term "phosphate-deficient soil" means a soil having a phosphoric acid content of less than 50 ppm.
In the present invention, the term "milky " means a period in which the seed germinates and leaves the weevil beyond 2 to 4 leaves. In the present invention, the term "rice paddy rice" means a period in which the rice seeds germinate and appear at about 2 to 4 leaves after the weaning period.
In one embodiment of the present invention, first, the rice genetic resources required for selection are selected, and rice seeds are best grown before breeding rice. In the present invention, the term " Choi "means to sow seeds of a seed with a little bit of seed for the purpose of promoting germination and growth in rice, and the" genetic resource " It means the whole of the gene.
In the present invention, the "growth" is performed in the greenhouse for 40 to 60 days. In one embodiment of the present invention, the rice seeds grown in the phosphate-deficient soil were grown in a greenhouse for 50 days. In the present invention, the growth is performed for 100-180 days in the packaging. In one embodiment of the present invention, the rice seeds most cultivated in the phosphate-deficient soil are seeded on a mat and grown for 30 days, Day.
The method of the present invention for determining stress tolerance to phosphorus deficiency in rice comprises the steps of: (a) measuring the dry weight of the rice seedlings; And (c) determining that the tolerance to phosphorous deficiency stress is higher as the measured weight of the rice plant is higher.
In the present invention, the term "under building" refers to the weight of a plant in which any growth part (ground part, underground part, assimilated part, non-assimilated part) or the whole is completely dried for growth analysis. In the present invention, in measuring the dry weight of the above-mentioned rice, the dry weight of the ground part except the roots was measured.
In the present invention, some seeds were sown in the greenhouse for 50 days after sowing the best-grown rice seedlings, and after 120 days of growth in the greenhouse, samples were collected to measure the weight of the rice seedlings. , And the correlation between them was analyzed.
As a result, in the untreatment treatment of nitrogen and potassium except phosphoric acid in phosphorus free loess according to the seedling standard application, there was a significant correlation (R 2 = 0.76) between the building of Yumiji rice and the building of mature rice It was confirmed that the building of mature rice paddy was also heavy when the building of rice paddy was heavy. As a result, it was predicted that the mature rice plants would be heavier than the rice plants by selecting the higher rice plants from the rice plants without growing them to the maturity stage. As a result, the heavier the rice plants were, And it was predicted that the resistance to high temperature would be high.
In addition, the correlation between the number of rice plants grown in the untreated rice field and the number of rice plants in the rice field was found to be highly correlated with the rice yield (R 2 = 0.79), indicating that rice yields (number of eggs) will also increase by confirming the structure of the rice seedlings without growing rice to maturity. In heavy cases, it could be predicted that the soil would be resistant to phosphate-deficient soil.
According to the present invention, it can be predicted that the resistance to phosphorus deficiency stress is higher when the rice is not grown up to the maturity stage and the measured weight of the rice seedlings is higher. Specifically, when selecting the rice that corresponds to the top 40% of the measured rice plants of Yumiji rice, the rice can be distinguished from the rice deficiency stress tolerant rice, but it is not limited thereto. Specifically, , And more specifically, when the rice of the top 20% among the buildings of Yumiji rice is selected, it can be discriminated as a phosphorus-deficient stress tolerant rice.
Therefore, according to the present invention, it is possible to determine the rice as a rice-deficient stress-tolerant rice by selecting the rice in the building of rice rice with a heavier weight, by measuring the weight of the rice in the rice field before the completion of growth of the rice. More specifically, The selected 40% of the selected rice plants can be selected as phosphorus deficient stress tolerant rice.
According to the present invention, the phosphorus-deficient stress tolerant rice, which is capable of securing growth even in a phosphorus-deficient environment, is not grown up to the maturity stage, In this study, it is possible to select rice-deficient stress-tolerant rice within a short period of time in a small area by analyzing the structure of rice seedlings at the stage of weeding, , And the degree of phosphorus-deficient stress tolerance of rice can be determined in advance.
Fig. 1 shows that the rice seedlings were divided into 20 chambers and 480 of the most genetically modified rice genetic resources were planted in the loess soil without phosphoric acid, nitrogen and potassium except for phosphoric acid in accordance with the standard fertilization rate, It shows the building of the ground part of the rice paddy.
Fig. 2 is a view showing 50 of the rice genetic resources selected from among the above-mentioned ground building of Fig.
FIG. 3 is a graph showing the correlation between the nitrogen content and the potassium content of phosphorus-free yellow clay, the nitrogen content and potassium content in the untreated untreated rice according to standard application rates, ).
FIG. 4 is a graph showing the correlation between nitrogen and potassium except for phosphoric acid in the conventional soil (low soil temperature), low phosphoric acid fertilizer applied in accordance with the standard fertilizer amount, and the structure of the rice in the mature stage Phosphate treatment).
Fig. 5 is a graph showing the correlation between phosphorus, nitrogen and potassium in the conventional soil and the standardized fertilizer application ratio in the mature rice and the mature rice plant (Practical treatment) .
FIG. 6 is a graph showing the correlation between the number of rice seedlings and the number of rice seedlings in the untreated rice field treated with untreated rice grown in the absence of phosphoric acid, nitrogen and potassium except phosphoric acid, according to standard application rates.
Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.
Example One: Unmanned mountain At the treatment A milestone Rice In building Measure
First, the rice genetic resources required for selection were selected, and the rice seeds were prepared most recently.
Thereafter, the hair growth box was divided into 20 chambers and filled with loess without phosphoric acid, and then 480 genetically modified rice genetic resources were grown in the greenhouse for 50 days after planting 10 laps each by one point in one seedling box. The phosphorus-nitrogen-potassium content in the hair growth box was 0-2-3 / box. After growing, samples were taken, dried and examined for dryness. As a result, the dryness of the top portion of the rice seedlings of 480 rice paddies of the best-known rice genome was the same as that shown in Fig. 1, and 50 points of genetic circle among the 480 points shown in Fig. 1 were selected. Respectively.
Example 2: A milestone In the building of rice and the maturity of rice In-building Correlation analysis
First, the rice genetic resources were prepared and prepared. For the purpose of the pavement test, the pavement was filled with unphosphorous soil and conventional soil (normal soil). Nitrogen and potassium except phosphoric acid were added to the standard soil (0-9-5.7 kg / 10a) (Low phosphoric acid treatment), nitrogen (phosphorus) and nitrogen (nitrogen) except for phosphoric acid were applied according to standard dosage (0-9-5.7 kg / 10a) , And potassium were applied to the test area (conventional treatment) according to the standard dosage (4.5-9-5.7 kg / 10a).
Thereafter, the most genetically modified rice genetic resources were sown on the seeding plate and grown for 30 days. After that, the seedlings were grown in 3 test pouches (uninfected fish paste, low phosphoric acid fish paste, conventional fish paste) (120 days). After analyzing the growth and agronomic traits of mature rice, we analyzed the correlation of building and agricultural traits according to phosphate fertilization in pavement.
Example 2-1: Unmanned mountain At the treatment A milestone In the building of rice and the maturity of rice In-building Correlation analysis
FIG. 3 is a graph showing the relationship between the nitrogen content and potassium content of phosphorus-free yellow clay in the untreated untreated rice according to the standard application rate, as compared with that of the rice grown in the mature rice plant Unmanned acid treatment).
As a result of the analysis of the correlation between the building of Yumiji rice and the building of mature rice, the correlation between the building of Yumiji rice and the building of mature rice was shown to be a significant correlation (R 2 = 0.76) Respectively. In this way, it was predicted that the mature rice plants would be heavier than the mature rice plants by confirming the structure of rice paddies without growing the rice to mature stage. In the case of heavy rice paddy, .
Example 2-2: Low phosphate At the treatment A milestone In the building of rice and the maturity of rice In-building Correlation analysis
FIG. 4 is a graph showing the relationship between the nitrogen content and the potassium content in the conventional soil (general soil), the nitrogen content in the soil of the mature rice plant (Low phosphate treatment).
As a result of analysis of the correlation between the building of Yumiji rice and the building of mature rice, there was not a significant correlation (R 2 = 0.42) between the building of Yumigi rice and the building of mature rice in the low phosphoric acid treatment area Respectively.
Example 2-3: Practices At the treatment A milestone In the building of rice and the maturity of rice In-building Correlation analysis
FIG. 5 is a graph showing the correlation between the composition of the soil of mature rice and that of mature rice in the conventional soil fertilized soil (general soil), phosphate fertilizer, nitrogen and potassium fertilized soil according to the standard fertilization rate Conventional treatment).
As a result of analysis of the correlation between the building of Yumigi rice and the building of mature rice, there was no significant correlation (R 2 = 0.29) between the buildings of Yumigi rice and the buildings of mature rice in the conventional treatment Respectively.
In conclusion, it was found that there was a significant correlation between the mature rice plant and the mature rice plant in the uninflammatory treatment area, but the correlation was weaker toward the low phosphoric acid treatment area and the conventional treatment area.
Example 3: Unmanned mountain At the treatment A milestone In the building of rice and the maturity of rice In-building Correlation analysis
Analysis of the correlation between the number of rice seedlings grown in the untreated rice field of Example 2-1 and the building of rice paddy rice showed that there was a high correlation between the rice yield of the rice paddy and the rice yield (R 2 = 0.79), confirming that the rice was not grown up to maturity and that the rice was grown in the mature stage, , And it was predicted that when the building of rice paddy was heavy, it would have tolerance in phosphate - deficient soil.
Therefore, it was confirmed that the rice grown in the untreated rice field after the completion of the growth can be easily screened in a short time in a small area, even in the phosphate deficient soil.
Claims (7)
(b) measuring the height of the rice paddy field; And
(c) predicting that the mature rice plant is heavily loaded or that the rice yield is high, and that the resistance to the phosphorus deficiency stress is high,
A method for determining stress tolerance of phosphorus deficiency in rice.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100770203B1 (en) * | 2005-12-19 | 2007-10-25 | 전북대학교산학협력단 | Method to improve plants phosphate uptake by increased Nicotiana tabacum phosphate transporter transgene expression and plants with improved phosphate uptake developed by the application of the method |
| JP4143380B2 (en) * | 2002-10-29 | 2008-09-03 | 日清製粉株式会社 | Grain characteristic measurement method and apparatus |
| KR20120089902A (en) * | 2010-12-20 | 2012-08-16 | 경기도 | Method of rice's detailed prescription use to digital image analysis |
| KR101509130B1 (en) * | 2010-05-19 | 2015-04-07 | 가부시끼가이샤 사따께 | Method for calculating weight ratio by quality in grain appearance quality discrimination device |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4143380B2 (en) * | 2002-10-29 | 2008-09-03 | 日清製粉株式会社 | Grain characteristic measurement method and apparatus |
| KR100770203B1 (en) * | 2005-12-19 | 2007-10-25 | 전북대학교산학협력단 | Method to improve plants phosphate uptake by increased Nicotiana tabacum phosphate transporter transgene expression and plants with improved phosphate uptake developed by the application of the method |
| KR101509130B1 (en) * | 2010-05-19 | 2015-04-07 | 가부시끼가이샤 사따께 | Method for calculating weight ratio by quality in grain appearance quality discrimination device |
| KR20120089902A (en) * | 2010-12-20 | 2012-08-16 | 경기도 | Method of rice's detailed prescription use to digital image analysis |
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