KR101700596B1 - Qtl analysis of stem diameter and caps marker therefor - Google Patents

Abstract

The present invention relates to a method for identifying single nucleotide polymorphisms (SNPs) specific to Milyang 23 and a preferred rice plant; Selecting a SNP located in a restriction enzyme site containing EcoR I, HindIII, Pst I and Xho I among the single nucleotide polymorphisms with a CAPS marker; Performing PCR using a primer specific to the CAPS marker; Preparing a genetic map shown in FIG. 3 using the PCR-separated CAPS marker; And analyzing a quantitative trait locus (QTL) related to the stem diameter of a recombinant subpopulation of Milyang 23 and a representative rice paddy on the basis of the genetic map; And a method for determining quantitative trait loci related to the stem diameter of a recombinant subpopulation of a representative rice paddy.

Description

{QTL ANALYSIS OF STEM DIAMETER AND CAPS MARKER THEREFOR}

The present invention relates to a composition for determining quantitative trait loci (QTL) associated with the stem thickness of rice.

Recently, the development of Next Generation Sequencing has resulted in a decrease in the cost of large-scale sequencing and acceleration of speed. In addition, when the rice genome is decoded, whole genome re-sequencing is performed based on a standard genome (reference sequence), and various markers such as SNP, insertion and deletion are mass- It can be developed in a short time. In China, a large number of SNPs were searched for by reabsence sequence analysis for native rice, and a high-density molecular genetic map was created and related genes for 14 major agricultural traits were reported (Non-Patent Document 2).

Agronomically important traits in crop breeding are not independent single genes but quantitative traits that are controlled by numerous genes on the entire chromosome. Quantitative traits are generally affected not only by a large number of genes but also by various environmental factors. Thus, in quantitative traits, it is extremely difficult to qualify traits such as individual gene actions or traits that affect them. Therefore, statistical methods have been used as a powerful tool for studying quantitative traits, and studies have been conducted by several scholars to effectively isolate genetic factors affecting quantitative traits from various environmental factors. The use of molecular genetic techniques for breed improvement will enable the study of the genetic predisposition of individuals at the DNA level, and it will be possible to identify genes related to the target traits to be improved, that is, major genes or loci of quantitative traits (QTL), or selection of genetic markers associated with quantitative trait loci (QTLs) can provide a tool for realizing a genetic locus. By using molecular genetic information rather than relying solely on phenotypic information, genetic improvement can be accelerated. Therefore, in order to study such a quantitative trait locus today, many approaches such as finding a gene through high density molecular genetic mapping and revealing its function are being performed. To date, various DNA markers have been developed, including restriction fragment-length polymorphism (RFLP), randomly amplified polymorphism (RAPD) and amplified fragment length polymorphism (AFLP) to generate high-density molecular genetic maps. In the rice, a high-density molecular genetic map consisting of 2,275 RFLP markers was prepared using Nipponbare, a Japonica variety and Kasalath mating F2 group, an Indica variety (Non-Patent Document 3), which was used for a rice genome decryption project.

Conventional DNA markers are not easy to identify, costly and take a long time. Moreover, the frequency of appearance is low. Recently developed SSR (Simple Sequence Repeat) and SNP (single nucleotide phymorphism) show much higher polymorphism among varieties or individuals, and they are widely used as markers because of their high utilization value because they are distributed evenly. However, SSRs are complex and difficult to identify because of small size of PCR products. SNPs using microarrays have the advantage of rapidly analyzing a large amount of samples, but development time and effort are needed.

Therefore, the present inventors developed a new CAPS marker using the next generation nucleotide sequence analyzing apparatus and used it for genetic and molecular mapping, and developed a QTL marker related to the stem size of rice.

1. Ji H et al. 2012. Development of rice genomic and physical maps using PCR-based DNA markers with the recombinant inbred population derived from Milyang23 / Gihobyeo cross. Korean J. Breed. Sci. 44: 273-281. 2. Huang X et al. 2010. Genome-wide association studies of 14 agronomic traits in rice landraces. Nature Genet. 42: 961-967 3. Harushima Y et al. 1998. A high-density rice genetic linkage map with 2275 markers using a single F2 population. Genetics 148: 479-494 4. Chen M et al. 2002. An integrated physical and genetic map of the rice genome. Plant Cell 14: 537-545 5. Cho YG et al. 2007. Identification of quantitative trait loci in rice for yield, yield components, and agronomic traits across years and locations. Crop Sci. 47: 2403-2417 6. Ashikari M et al. 2002. Loss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice 'green revolution'. Korean J. Breed. Sci. 52: 143-150.

It is an object of the present invention to provide a CAPS marker composition for determining quantitative trait loci (QTL) related to the stem diameter of a recombinant subpopulation of Milyang 23 and a representative rice paddy.

Another object of the present invention is to provide a genetic map for determining the Quantitative Trait Locus (QTL) associated with the stem thickness of the recombinant subpopulations of Miryang No.23 and Rice.

It is another object of the present invention to provide a method for determining quantitative trait loci (QTL) related to the stem diameter of a recombinant subpopulation of Milyang 23 and a representative rice paddy.

Another object of the present invention is to provide a kit for determining the quantitative trait locus related to the stem diameter of the recombinant subpopulations of Milyang 23 and the representative rice paddy.

It is yet another object of the present invention to provide quantitative trait loci (QTL) associated with stem diameters determined by the method.

In order to achieve the above object, the present invention provides a genetic map for determining quantitative trait loci related to the stem thickness of the recombinant subpopulations of Milyang No. 23 and Tong Rice.

As used herein, the term " rice silage 23 " is used interchangeably herein as " rice silicate 23 / The above-mentioned " rice silage No. 23 " is a group of 162 child strains fixed by progressing over 20 generations. There is no restriction on the extraction of DNA and it is possible to repeatedly experiment by various environments and periods Lt; / RTI >

As used herein, the term " quantitative trait loci (QTL) " refers to a trait that is expressed by variation of alleles present in a plurality of loci, such as stem thickness.

As used herein, the term " quantitative trait locus analysis " may be based on searching for markers associated with a phenotype between groups that exhibit characteristics that are contrasted for a particular trait. Through the QTL analysis, it is possible to investigate the degree of each gene locus expression, interaction between loci, and genetic loci by environment.

As used herein, the term " cleaved amplified polymorphic sequence (CAPS) marker " refers to a SNP located in a restriction enzyme site among single nucleotide polymorphisms (SNPs). In the embodiment of the present invention, the CAPS markers are selected from SNPs located in the restriction enzyme sites (EcoRI, HindIII, PstI, XhoI) among the SNPs specific to the two rice varieties 23 and the representative rice species present in the coding region sequence (CDS) It can be developed as a candidate CAPS marker.

One of the major traits of rice is the thickness of the rice, which is related to the productivity improvement of rice. The smaller the thickness of the stem, the weaker the resistance to environmental factors such as the weight of the grain itself and the wind and rain, and it is easy to collapse. As a result, if the stem is fallen, the anabolic dysfunction and nutrient migration are inhibited, resulting in reduced yield and poor quality of rice. Thus, in order to carry out the QTL study on the stem-thickness trait, the present invention was developed to develop cleaved amplified polymorphic sequences (CAPS) markers for specific SNPs between Milyang 23 and rice paddies through a next-generation mass sequencing analysis, This map is integrated with the constructed MGRIL genetic map (non-patent document 1), and provides a map that allows the analysis to be easily and uniformly distributed among the markers.

In an embodiment of the present invention, the map may be a genetic map as shown in Fig. 3 for determining the quantitative trait locus associated with the stem thickness of the recombinant subpopulation of Milyang No. 23 and a representative rice paddy.

In an embodiment of the present invention, the map may be the physical map shown in Fig. The physical map can be created by grasping the physical position of the rice marker between the DNA markers on the rice rice genome.

In the embodiment of the present invention, the genetic map was developed as a cleaved amplified polymorphic sequences (CAPS) markers for specific SNPs between Milyang No. 23 and representative rice paddies through a next-generation mass sequencing analysis in order to carry out a QTL study on stem diameter And then integrated with the MGRIL genetic map consisting of PCR-based markers (Non-Patent Document 1), so that the analysis can be easily made and can have an even distribution among the markers.

The present invention also relates to a method for the detection of single nucleotide polymorphisms (SNPs) in a mammal, 2) selecting a SNP located in a restriction enzyme site containing EcoR I, HindIII, Pst I and Xho I among the above single nucleotide polymorphisms with a CAPS marker; 3) performing PCR using a primer specific to the CAPS marker; And 4) analyzing the quantitative trait loci (QTL) related to the stem diameter of the recombinant subspecies of Milyang 23 and the rice paddy on the basis of the genetic map, and the stem of the recombinant subpopulation of the rice paddy Provides a method to determine quantitative trait loci related to thickness.

In the embodiment of the present invention, step 1) can be developed by analyzing the genomic sequence of Milyang 23 and the rice seedlings based on the recently developed next generation sequencing (NGS) no. In the embodiment of the present invention, the above steps 1) and 2) determine the nucleotide sequence of the Nipponbare genome by NGS at a multiple of 60 times the length of the Nipponbare genome, and SNPs that are specifically expressed between the two cultivars in the CDS can be used as CAPS markers. The step 3) can preferably perform PCR using the primer sequences shown in Table 4 of the present invention. In the embodiment of the present invention, in step 4), 146 CAPS markers newly developed in the present invention and 219 previously reported markers are integrated into a total of 365 markers, Molecular genetic map can be created for. In an embodiment of the present invention, step 4) can be determined by searching for QTL regarding the stem thickness of rice.

The present invention also provides a method for determining stem diameter, said method comprising the steps of: 5) determining stem diameter related quantitative trait loci (QTL) qI1D1, qI1D5, qI3D1 and qI4D1; And a method for determining a quantitative trait locus related to the stem diameter of a recombinant subpopulation of a representative rice paddy.

The present invention also provides qI1D1, qI1D5, qI3D1 and qI4D1, which are the stem-related quantitative trait loci (QTL) determined by the method described above.

As a result of the QTL analysis in the embodiment of the present invention, a total of 19 significant QTLs were found. Among these, QTLs related to four stem thickness traits were new QTLs that were not previously reported. QI1D5, which has the largest LOD value of the stem length QTL, was found on chromosome 5, and the variation in the one - fold thickness phenotype was 8.99%. Only a small number of SNPs identified through the recombinant sequence were used in this study. If SNP information revealed in this study is used in future, more marker can be developed and high density genetic mapping can be made. Furthermore, MGRIL can be used to enable more accurate QTL analysis and development of useful genes for agriculturally useful traits.

The present invention also provides a kit for determining a quantitative trait locus related to the stem diameter of a recombinant subpopulation of Milyang 23 and the rice paddy including the CAPS marker.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

By using the SNP information and the CAPS marker of the present invention, more markers can be developed, and high density genetic mapping can be made in the future. In addition, the QTL markers related to the stem-like traits developed in the present invention are effective for molecular breeding.

FIG. 1 shows the results of analysis of SNP, insertion, and deletion between the Nipponbare sequence according to the embodiment of the present invention, the Milyang 23 and two representative rice paddies. PCR product size was 585 bp, and alleles of Milyang 23 and rice paddy rice were digested with restriction enzymes 230 bp and 350 bp, respectively (MY: Milyang 23, GH: rice paddy).
Figure 2 shows a physical map depicting the physical location of a marker in accordance with embodiments of the present invention. Chromosome numbers are indicated on each map. The name of each marker is shown on the right side of the chromosome, and the physical distance from the top of each chromosome to the first marker is shown on the left. The physical distance unit is Mbp (Megabase pair).
Figure 3 shows the genetic map of a recombinant subpopulation of Milyang 23 and a representative rice paddy according to an embodiment of the present invention. Chromosome numbers are indicated on each map. The name of each marker is shown on the right side of the chromosome, and the genetic distance from the first marker from the top of each chromosome is shown on the left. The genetic distance unit is cM (centimorgan), which is calculated by the Kosambi function.
FIG. 4 is a graph showing the relationship between the 1 st thickness (I1D), the 2 st thickness (I2D), the 3 st thickness (I2D), and the 3 rd thickness of the Milyang 23, I3D), 4th section (I4D), liver (CL), and shoot (PL) phenotypes. Rice seeds and Milyang No. 23 are shown on the graph.
Figure 5 shows the stem thickness and the location of the QTL associated with liver traits measured in a recombinant control group of Milyang No. 23 and a representative rice paddy according to an embodiment of the present invention. The QTL location for the first section thickness (I1D), third section thickness (I3D), fourth section thickness (I4D), and liver (CL)

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Materials and methods

1. Genetic analysis group and Genomic DNA  extraction

The genetic analysis group used in this study consisted of 162 recombinant lines (Recombinant Inbred Lines), which were mated with Milyang 23 and Japonica, respectively. This group was crossed in 1988 and F2 was fused to F6 generation by single seed descent (SSD) method. Then, F7 generation was used to grow F10 seedlings per line, Respectively. The young leaf tissue of each strain was sampled to extract genomic DNA. The method was as previously reported in Non-Patent Document 1.

2. NGS  Mass sequencing

Milyang 23 and the leaves of the seed rice were harvested and DNA was extracted according to the manual provided by DNeasy Plant Maxi Kit (Qiagen). The extracted DNA was used for the next generation sequencing. Paired-end sequencing was performed using Illumina Hiseq 2000 for the sequencing of the rice samples. The length of the generated leads was 101 bp. The lead used a Phred Quality Value ≥ Q20 with an error rate of less than 1%. The nucleotide sequences of the two samples were assembled on a standard dielectric (Nipponbare) using a CLC Assembly Cell (ver. 3.3.2, http: //www.clcbio.com) and analyzed for mutation.

The standard genome is Oryza sativa L. cv. Nipponbare (MSU Rice Genome Annotation Project, Pseudomolecules Build 7.0), and annotation information for analyzing the genome structure was RAP-DB (http://rapdb.dna.affrc.go.jp/).

3. SNP primer  Production and PCR  Perform

SNPs (EcoRI, HindIII, PstI, XhoI) located in the CDS (coding sequence) in the genomic DNA sequence of the decoded parents by the next-generation sequencing method and four As a candidate CAPS marker. After that, the primers were prepared using the Vector NTI 9.0.0 program based on the selected SNPs (Table 4).

For the PCR reaction, the total volume of the reaction solution was adjusted to 20 μl, and 2 μl of 10 × Buffer, 0.4 μl of dNTP and 0.5 μl of Taq polymerase were used. The PCR amplification conditions were denaturation at 95 ° C. for 5 minutes, , 58 ° C to 62 ° C for 30 seconds, and 72 ° C for 1 minute and 30 seconds, and the final extension was performed at 72 ° C for 10 minutes.

4. CAPS Marker  Development and Genotype Analysis

PCR was performed with the SNP primer previously developed with Milyang No. 23 and gDNA of rice paddy rice, followed by 5 μl of PCR product, 1 μl of 10 × buffer, and 5 μ of restriction enzyme. Lt; / RTI > After restriction enzyme treatment, the cells were electrophoresed on 1.2% agarose gel, and polymorphism was determined. Genetic analysis of the MGRIL population was performed with the polymorphic markers.

5. Physical and Genetic Mapping

In order to make physical mapping of this group, 219 PCR-based STS, InDel and SSR markers (non-patent document 1) were integrated with the CAPS markers developed. Using the primer sequence information and position information of DNA markers, the position on the rice genome was determined and physical map was created. For this, the location information of the standard dielectric Nipponbare (build 7.0) was used.

Genotype data were analyzed using the MapDisto program (Mathias 2012) for genetic mapping. After entering the genotype of the MGRIL population, the genetic distance between the markers of each association group was calculated using the "Draw all sequence" menu, and the genetic map was created. At that time, the Kosambi function formula was applied. The final genetic map was used by the MapChart program (Voorrips 2002).

6. phenotype investigation and QTL  analysis

For the QTL analysis, stem diameter, culm length and panicle length of the test materials were investigated. Stem thickness was determined by dividing a total of 4 segments into 5 segments per line using vernier calipers with a first internode, a second internode, a third internode, and a fourth internode. Respectively. The distance between the head of the ears and the first node is 1, and the distance between the nodes is 2, 3 and 4, respectively. The thickness of the stem was measured at the center between the nodes. QTL analysis was performed using Windows QTL Cartographer V2.5, and composite interval mapping (CIM) was used for analysis. Significant LOD (logarithm of the odds) value was adopted by performing 1,000 replications at 95% significance level for each trait.

Example  One. NGS  The mass sequence and / mapping

Repeated sequence analysis was performed on the Milyang 23 and the Nipponbare sequence (373 Mbp) of the recombinant genetic group. As a result, the sequence of Milyang 23 was determined to be 69.6x compared to the Nipponbare sequence and 68.5x for the representative rice (Table 1). The average depth of the readings mapped to the Nipponbare sequence, which is a standard genome, was 55.7x for Milyang 23 and 63.3x for pseudomonas, and the mapping area between the two samples and Nipponbare The coverage was 78.3% for Milyang 23 and 90.5% for rice, respectively (Table 2).

Sequence analysis of Milyang 23 and rice seedlings Variety Total Bases
( bp ) Depth  (X) Read Count GC  (%) Q20 ^z  (%) Q30 ^y  (%) Milyang23 26,590,233,034 69.6 263,269,634 44.92 93.26 86.27 Gihobyeo 26,150,864,147 68.5 258,919,447 43.82 93.45 86.51

^z : A quality score of 20 represents an error rate of 1 in 100, with a corresponding call accuracy of 99%.

^y : A quality score of 30 represents an error rate of 1 in 1000, with a corresponding call accuracy of 99.9%

Read mapping and assembly results on Nipponbare standard dielectric Variety Mapped reads
(#) Mapped reads
(%) Depth
(X) All mapped nucleotide
( bp ) Coverage
(%) Milyang23 205,997,634 78.25 55.7 344,161,476 92.05 Gihobyeo 234,289,038 90.49 63.3 365,807,427 97.84

Example  2. Mutation search and candidates CAPS Marker  Development

(SNP, Insertion, Deletion) between the Nipponbare sequence and the two samples. There were 1,564,167 and 162,631 varieties detected in Milyang 23 and Rice, respectively. Among them, the specific variation between two varieties were Milyang 23, 1,237,160, and Rice 87,271. The difference of these mutations is considered to be the difference in the nucleotide sequence of Japonica and Nipponbare, a varieties of Japonica and Indica, and Miryang 23, a varieties of Japonica, unlike those of Japonica. These mutations are classified into un-translated region (UTR), intron, inter-region and coding region sequence (CDS) according to the genome structure information provided by RAP-DB. The SNPs located in the restriction sites (EcoRI, HindIII, PstI, XhoI) of the two SNPs in the CDS were selected as candidate CAPS markers (Table 3, Table 4). Finally, 146 SNPs were used for molecular label development (Figure 1).

Milil 23 and the rice seed specific CAPS marker sequence information C hr P os marker _ name M ilyang23 G iHO Gene _ ID r egion e nzyme Chr1 1601850 RS011 G A LOC_Os01g03820.1 CDS Hind3 Chr1 24766160 RS0110 C T LOC_Os01g43330.1 CDS Hind3 Chr1 25370780 RS0111 G C LOC_Os01g44250.1 CDS Pst1 Chr1 27941461 RS0112 G C LOC_Os01g48720.1 CDS Pst1 Chr1 29459989 RS0113 G A LOC_Os01g51250.1 CDS EcoR1 Chr1 29617209 RS0114 T C LOC_Os01g51560.1 CDS Xho1 Chr1 30781803 RS0115 C T LOC_Os01g53610.1 CDS Hind3 Chr1 31358216 RS0116 A C LOC_Os01g54515.1 CDS Pst1 Chr1 33101571 RS0117 C G LOC_Os01g57280.1 CDS Hind3 Chr1 36486669 RS0118 A C LOC_Os01g62990.1 CDS Pst1 Chr1 38066048 RS0119 G A LOC_Os01g65580.1 CDS Hind3 Chr1 1959253 RS012 G C LOC_Os01g04409.1 CDS Xho1 Chr1 39389883 RS0120 T C LOC_Os01g67770.1 CDS Hind3 Chr1 39596921 RS0121 A G LOC_Os01g68120.1 CDS Xho1 Chr1 40596478 RS0122 C T LOC_Os01g70140.1 CDS EcoR1 Chr1 40986838 RS0123 C T LOC_Os01g70810.1 CDS Pst1 Chr1 42328372 RS0124 G T LOC_Os01g72970.1 CDS Pst1 Chr1 42552002 RS0125 T C LOC_Os01g73430.1 CDS Xho1 Chr1 3225214 RS013 T C LOC_Os01g06790.1 CDS Xho1 Chr1 4487164 RS014 T G LOC_Os01g08930.1 CDS Xho1 Chr1 7016670 RS015 A C LOC_Os01g12700.1 CDS Hind3 Chr1 11090143 RS016 T C LOC_Os01g19548.1 CDS EcoR1 Chr1 14812838 RS017 G A LOC_Os01g26160.1 CDS EcoR1 Chr1 22037567 RS018 G A LOC_Os01g39150.1 CDS Hind3 Chr1 22044959 RS019 C T LOC_Os01g39180.1 CDS Xho1 Chr2 2960072 RS0226 T G LOC_Os02g05950.1 CDS Pst1 Chr2 3953069 RS0227 C T LOC_Os02g07630.1 CDS Pst1 Chr2 6472561 RS0228 A G LOC_Os02g12400.1 CDS Pst1 Chr2 7317708 RS0229 A G LOC_Os02g13640.1 CDS Pst1 Chr2 8867251 RS0230 C G LOC_Os02g15730.1 CDS Pst1 Chr2 9563627 RS0231 C A LOC_Os02g16760.1 CDS Hind3 Chr2 10555862 RS0232 T C LOC_Os02g18180.1 CDS Xho1 Chr2 12402078 RS0233 C T LOC_Os02g20970.1 CDS Pst1 Chr2 16414962 RS0234 G A LOC_Os02g27710.1 CDS Pst1 Chr2 17886097 RS0235 T A LOC_Os02g30110.1 CDS Xho1 Chr2 18228684 RS0236 T C LOC_Os02g30620.1 CDS Hind3 Chr2 18237931 RS0237 T G LOC_Os02g30630.1 CDS Xho1 Chr2 19790251 RS0238 T C LOC_Os02g33310.1 CDS Hind3 Chr2 21101992 RS0239 G A LOC_Os02g35140.1 CDS EcoR1 Chr2 23436255 RS0240 G C LOC_Os02g38780.1 CDS Xho1 Chr2 23658021 RS0241 C G LOC_Os02g39160.1 CDS Xho1 Chr2 25320749 RS0242 A G LOC_Os02g42110.1 CDS Pst1 Chr2 32186260 RS0243 A T LOC_Os02g52610.1 CDS Pst1 Chr2 33194196 RS0244 A G LOC_Os02g54150.1 CDS Xho1 Chr2 33630297 RS0245 G A LOC_Os02g54910.1 CDS Pst1 Chr2 34766191 RS0246 A G LOC_Os02g56700.1 CDS Pst1 Chr3 4436656 RS0347 C T LOC_Os03g08610.1 CDS EcoR1 Chr3 4942068 RS0348 G A LOC_Os03g09920.1 CDS EcoR1 Chr3 5818943 RS0349 T G LOC_Os03g11310.1 CDS EcoR1 Chr3 10300785 RS0350 C T LOC_Os03g18380.1 CDS EcoR1 Chr3 10758304 RS0351 T A LOC_Os03g19190.1 CDS EcoR1 Chr3 16184967 RS0352 T C LOC_Os03g28140.1 CDS Xho1 Chr3 24431649 RS0353 T C LOC_Os03g43684.1 CDS Pst1 Chr3 26992096 RS0354 G A LOC_Os03g47650.1 CDS Hind3 Chr3 33689816 RS0355 T C LOC_Os03g59160.1 CDS Pst1 Chr3 36119119 RS0356 T A LOC_Os03g63920.1 CDS Hind3 Chr4 6638464 RS0457 C A LOC_Os04g12080.1 CDS Hind3 Chr4 12602077 RS0458 G A LOC_Os04g22240.1 CDS EcoR1 Chr4 13512519 RS0459 A G LOC_Os04g23600.1 CDS EcoR1 Chr4 17876462 RS0460 G A LOC_Os04g29960.1 CDS EcoR1 Chr4 17888569 RS0461 G A LOC_Os04g29990.1 CDS Xho1 Chr4 20589612 RS0462 G A LOC_Os04g34000.1 CDS Xho1 Chr4 21552430 RS0463 A G LOC_Os04g35420.1 CDS Xho1 Chr4 23772074 RS0464 A G LOC_Os04g39910.1 CDS Xho1 Chr4 27784875 RS0465 C T LOC_Os04g46880.1 CDS EcoR1 Chr4 30726432 RS0466 G A LOC_Os04g51820.1 CDS EcoR1 Chr4 31554999 RS0467 G T LOC_Os04g52970.1 CDS EcoR1 Chr4 31833178 RS0468 C A LOC_Os04g53460.1 CDS Hind3 Chr4 32824390 RS0469 C G LOC_Os04g55210.1 CDS EcoR1 Chr4 34273550 RS0470 A G LOC_Os04g57600.1 CDS Xho1 Chr4 34451488 RS0471 C T LOC_Os04g57860.1 CDS Hind3 Chr5 4309461 RS0572 A C LOC_Os05g07950.1 CDS Pst1 Chr5 6884680 RS0573 G T LOC_Os05g12040.1 CDS EcoR1 Chr5 6938692 RS0574 A G LOC_Os05g12140.1 CDS Xho1 Chr5 18564584 RS0575 A G LOC_Os05g31890.1 CDS Xho1 Chr5 19449628 RS0576 G C LOC_Os05g33160.1 CDS Xho1 Chr5 21706257 RS0577 C G LOC_Os05g37140.1 CDS Xho1 Chr5 22840944 RS0578 C T LOC_Os05g38950.1 CDS Pst1 Chr5 24076697 RS0579 G A LOC_Os05g41100.1 CDS Hind3 Chr6 4333751 RS0680 A G LOC_Os06g08690.1 CDS Xho1 Chr6 7407888 RS0681 T C LOC_Os06g13460.1 CDS Pst1 Chr6 11015790 RS0682 C T LOC_Os06g19360.1 CDS Xho1 Chr6 11089700 RS0683 A G LOC_Os06g19470.1 CDS EcoR1 Chr6 12796996 RS0684 T G LOC_Os06g22060.1 CDS EcoR1 Chr6 16265449 RS0685 T C LOC_Os06g28590.1 CDS EcoR1 Chr6 18931341 RS0686 A G LOC_Os06g32550.1 CDS Pst1 Chr6 20582800 RS0687 T A LOC_Os06g35300.1 CDS EcoR1 Chr6 22375507 RS0688 A G LOC_Os06g37810.1 CDS Pst1 Chr6 23139245 RS0689 C T LOC_Os06g38980.1 CDS Hind3 Chr6 25730803 RS0690 A T LOC_Os06g42800.1 CDS EcoR1 Chr6 27232021 RS0691 C T LOC_Os06g45020.1 CDS EcoR1 Chr6 28832535 RS0692 C T LOC_Os06g47620.1 CDS Hind3 Chr6 29734206 RS0693 T G LOC_Os06g49060.1 CDS Xho1 Chr7 16326841 RS07100 C T LOC_Os07g27980.1 CDS Pst1 Chr7 25687783 RS07101 T C LOC_Os07g42900.1 CDS Hind3 Chr7 29124412 RS07102 G T LOC_Os07g48640.1 CDS EcoR1 Chr7 945242 RS0794 C A LOC_Os07g02620.1 CDS Pst1 Chr7 3561493 RS0795 T C LOC_Os07g07194.1 CDS Xho1 Chr7 3866257 RS0796 A G LOC_Os07g07690.1 CDS Pst1 Chr7 6019346 RS0797 T C LOC_Os07g10970.1 CDS Xho1 Chr7 9252607 RS0798 A G LOC_Os07g15930.1 CDS Pst1 Chr7 14539888 RS0799 T C LOC_Os07g25440.1 CDS Xho1 Chr8 1198055 RS08103 T C LOC_Os08g02850.1 CDS Xho1 Chr8 2024065 RS08104 T C LOC_Os08g04170.1 CDS Xho1 Chr8 8788708 RS08105 C G LOC_Os08g14610.1 CDS Hind3 Chr8 12081057 RS08106 T C LOC_Os08g20160.1 CDS Hind3 Chr8 13473581 RS08107 C T LOC_Os08g22354.1 CDS Xho1 Chr8 17452677 RS08108 A G LOC_Os08g28570.1 CDS EcoR1 Chr8 21839328 RS08109 T C LOC_Os08g34740.1 CDS Xho1 Chr8 27025915 RS08110 A G LOC_Os08g42710.1 CDS Xho1 Chr8 27694486 RS08111 T C LOC_Os08g43980.1 CDS Xho1 Chr8 27902691 RS08112 A G LOC_Os08g44340.1 CDS Xho1 Chr9 415553 RS09113 G T LOC_Os09g01590.1 CDS Pst1 Chr9 6248346 RS09114 C A LOC_Os09g11250.1 CDS EcoR1 Chr9 10154341 RS09115 C T LOC_Os09g16540.1 CDS Pst1 Chr9 16479088 RS09116 C T LOC_Os09g27080.1 CDS EcoR1 Chr9 17988201 RS09117 C T LOC_Os09g29584.1 CDS EcoR1 Chr10 1746230 RS10118 A T LOC_Os10g03850.1 CDS EcoR1 Chr10 2662340 RS10119 A G LOC_Os10g05400.1 CDS EcoR1 Chr10 3474938 RS10120 G C LOC_Os10g06710.1 CDS Xho1 Chr10 11700655 RS10121 T C LOC_Os10g22570.1 CDS EcoR1 Chr10 16038003 RS10122 T C LOC_Os10g30790.1 CDS Pst1 Chr10 16853254 RS10123 G T LOC_Os10g32080.1 CDS Pst1 Chr10 19417856 RS10124 C A LOC_Os10g36350.1 CDS Hind3 Chr10 20284416 RS10125 T C LOC_Os10g37880.1 CDS Pst1 Chr11 1995714 RS11126 A G LOC_Os11g04680.1 CDS Hind3 Chr11 2126111 RS11127 C G LOC_Os11g04960.1 CDS Hind3 Chr11 3590980 RS11128 C A LOC_Os11g07180.1 CDS Hind3 Chr11 5088240 RS11129 C T LOC_Os11g09474.1 CDS Hind3 Chr11 5602009 RS11130 G C LOC_Os11g10310.1 CDS Hind3 Chr11 7603935 RS11131 T C LOC_Os11g13810.1 CDS Hind3 Chr11 15694355 RS11132 A T LOC_Os11g27264.1 CDS Hind3 Chr11 15791084 RS11133 C G LOC_Os11g27430.1 CDS Pst1 Chr11 21176969 RS11134 A T LOC_Os11g36020.1 CDS Hind3 Chr11 25433209 RS11135 A G LOC_Os11g42220.1 CDS Hind3 Chr11 26214259 RS11136 A G LOC_Os11g43410.1 CDS Pst1 Chr11 26954605 RS11137 T A LOC_Os11g44580.1 CDS Pst1 Chr12 1561008 RS12138 G A LOC_Os12g03816.1 CDS Hind3 Chr12 1804345 RS12139 T G LOC_Os12g04260.1 CDS Pst1 Chr12 4217458 RS12140 T A LOC_Os12g08280.1 CDS Hind3 Chr12 18470703 RS12141 T G LOC_Os12g30760.1 CDS Pst1 Chr12 19755602 RS12142 T C LOC_Os12g32710.1 CDS EcoR1 Chr12 24714226 RS12143 T G LOC_Os12g39980.1 CDS Pst1 Chr12 26005435 RS12144 G A LOC_Os12g41950.1 CDS Pst1 Chr12 27401061 RS12145 G A LOC_Os12g44170.1 CDS Hind3 Chr12 27095713 RS12146 G A LOC_Os12g43630.1 CDS EcoR1

* reference: Nipponbare genome (IRGSP build 7.0)

The primer sequence for detecting the CAPS marker of the present invention and the PCR information marker _ name Forward _ primer  (5'-3 ') Reverse _ primer  (3'-5 ') Pro.len ( bp ) annealing tmp (° C) RS011 TCCAGAAGTCAGGATAGGAGTGGCA TGGAAGAACCACAACTGCATCATG 683 59 RS0110 TGAAACCCTTCTTCAGGAAACCAGC CATTGTGCAGCCAGGAAAAGGATAA 747 58 RS0111 CAGTGCAGCGATCTTTATCAGCTTC TCGATCCTCGAGTCCATGCATG 444 58 RS0112 TCCTGCTTTTATGTTGGCTGTCACT GGCTGCTGCTTCAAAATTCCCTTT 783 58 RS0113 TGCATTTTGGAGCATTGTAGACGC TGCAAGCACAGAAGTGGGCAGT 679 58 RS0114 CACACGCAGCGCAGAAACTAAAA CCATCGAAAATCCACTGCAACATT 711 58 RS0115 AATGGTCTTGGTGACGAAGCAACTG CGAATAGGTGACCGAGCAGCACT 701 58 RS0116 TGCAGCGCCATCATCATGAGC AGTTGAGGAAGCTGAGCACGGC 658 58 RS0117 AGGGATTAATTGATTCACACAGCCC CCCAGTTGATGCAACTCATATCCCT 730 58 RS0118 ATATGATGTCACCCAAAGTTGCCCT GTGTTCCGTGTTCTTGATTTCGTGT 696 58 RS0119 GGGACATTGGCTTGACACATAAGTG ACGAACACTCTGGCAAGGACCA 682 58 RS012 TTGTTCTTGTAAAGGCGCTTCACTG ACTGTCCTTCGTTTCAACAGGGG 753 58 RS0120 ACACGTGCTGCCAAATATTGCG TTTTGCAGTCCTGCAGACCATTTAA 731 58 RS0121 TTTACCAAGCAGGGGATCAGAAATG TGCATTGCATTACCTGTGTCCTTTT 716 58 RS0122 GGCAGAACAGGAGCATTAGCAAGC CCTGGACTGGACTGGAGTATAGGGA 800 61 RS0123 CTGCAACTCAATGGCTCCTAGGTG GCTGGCAGAGCTTTATGTAATGGGA 804 58 RS0124 CAGGAGGAAAACACACCGGATGG CCGTCCACTGTAAGAACTGAATGCC 708 59 RS0125 ATGCGGAGGAAATGCCAAACA GGATCGATGCGTGAAATATTGAAGA 848 55 RS013 ATCAGGGAGTTTGCAATCAAAATGG CCCTTGTGGTATCTGTCCAGTCAAA 439 58 RS014 TCAGAAGATTGTACCTTTGGCTCCC GGGAAGTTCCTGCTGAAGAGAGGTA 723 58 RS015 TGTGTTCGTGATAGGATGCACAGG CGCACCTCCCCTCCATGTAAA 527 58 RS016 ATCCCATCCACATCCTTTGAATCC CGAGGTCAAGAGAATCTGGGAGTCT 432 62 RS017 TCAGCAAATGGAGTTGGCTAAACAA AGTGATGGAGAGGCCAAAATGTCTT 762 58 RS018 TGAAGGGGCTGATCGAGCTCCA TGGATCCGGCGACGACTACTTATT 502 58 RS019 ACTTCATGGGGACGAGAAGGTACG CATAGGTAGGAGGCACACATCCGT 703 55 RS0226 TGAAGCTGCTTCTAAATCACCATCG CTCACAGGACCAATACCAAGATGGA 753 58 RS0227 ATCTGGCTCAAGACTGTCCCTGATC GGCACCACTGCATCATACGTCTACT 685 62 RS0228 GAGATGTTTAGCGGGAAGAGACCAA GGATGGAATCCTGCGGGTAGTTAA 703 62 RS0229 GAAGTTGCAAAGATGGAGGAACTCG TGCATGGCCAGTGAGGATGGT 678 62 RS0230 AAATAAAAGACAAACGGGGAGCACA CAAAACCGAATCGCCCCTATAAATT 707 58 RS0231 CTGCTGGCCAGCGTTCTACAAG CCAACCGCAAATCAGCCTACAA 690 58 RS0232 TTGGGCTGAAGCAAAGAGCGA CGTCTGCTGGAAGACAAAATGAAGG 761 58 RS0233 TGTCATCTGGAAGTATGGAGGCTCA TGTTGCTCTAGCTGGGGCATTAAAT 814 62 RS0234 AAAATGGTGCACGAAGCAATTGATG CCCCTGCTGAAAAAGGAAAATGAG 684 58 RS0235 AGCATCTTAATAGGTTTGCGGATGG CAAACACGGGAGCTCTCCATACATT 748 55 RS0236 TACCTGAATCACAACCTTCTGTGCC CCTTTGGCTGCAATATGTCTTGTTG 692 58 RS0237 CTGGCATTGATCCGCATTGAGA TCGTGTGATGACAGTTGAGCAAACA 739 58 RS0238 CGCTGACATAGCAAATCTGATCTCC CCGGTCGAGAGAAGCTCTGTAGAAG 689 58 RS0239 AGAAAATGCCTGCAGTTGTGTTGTC TTCCAAACAATCTGCAGCCAGC 680 58 RS0240 GGAAGTCATCCTGATCCAGCTTGTG ACGCCTCCAGATTCAAGCAAGAG 702 58 RS0241 GCCAAGTGGCACATTCCCAAC AGCCGGAAGGATACACGAATCAG 876 58 RS0242 TGTTAATGAGGTTGCCATCTTGTCG CCATTACAGAAATGGGTTGGCACTT 743 62 RS0243 TTGACAGTACATGCTGGGTTAGGGA GCAGCTTTTCATTCTGAGCGCA 679 59 RS0244 TTCCAGAGTAGCAGCATCTTGTGCA TGGTTTTAGTGGAGGTGATTCGTTG 719 58 RS0245 TGTACGGCTACACAGCTGAAATTCC ACCATTTTCCGAACTGCTTTCTTTC 763 59 RS0246 ACGATTATTGCCATGACAAGATCGA GGTTTGTGTTCTCCCACTCTCCAA 729 59 RS0347 TTCGAAAATAAAAACCCTGCTCCAG CCTCAAAATGCCTTTCCAAATTCAG 770 58 RS0348 TTCACTTTCCTCCTCACTTTCGTCA GGCAGTGCATTTTGGACACACAGTA 758 58 RS0349 TGGCTTGTTTTGTGCGAAGGG CCCATTGACCAAGTCTTTCACAAGA 768 58 RS0350 TCTCAATCTTAACCCCACTCTTCGG TGACCACTGTCTGACATGGGTC 682 58 RS0351 TCAAAATTCTGTGAAGACCGATGGA CAACAATTTGCCAACCTCATTGC 713 58 RS0352 TTTCGCAATACAAGTAGGACTGCCA GTGGTGTCGCTCAAACAGGTTTG 724 58 RS0353 ATCTAGCAGTCGATTGTTGGCATTG TGGTGCTGTAATTCCATTTCCATCA 720 59 RS0354 TTGCTGCCTCCAGTTGGAAGTCT CCCAACAGCAAGCATACACACACTC 607 58 RS0355 TGACCAGCATCAGCATATCAAAACA GCAGCAAGGTCATGATGACCATATT 603 59 RS0356 ATGTAATCCATGCCCCTTATTTTGC TCAGAGCTTCTGGAGGTGAGGAATT 674 58 RS0457 CATCATTGTGCAGGTATGGGAGAAA CCCGCTTAAATCGTCCCTTTTGTA 851 58 RS0458 GATGATGAAAATGACACTGCTTGGG GCAAAGTAAACAGGGCAAGCAAAA 724 58 RS0459 AGCAATTTTTCGCCTGCATTCC TGGAACATGCCATTCATTGACAGAG 678 58 RS0460 ATAGGAAAATTCACCTCGACAGCCG TGGCAGCTCAACATGACTGGC 585 59 RS0461 CCTGGAACAACCAAACACTGACTTG CGCCGTCGCAATTAAAATGTGC 678 59 RS0462 GGAACTGGCTACAGGAAAGACTTGG AGCCCTCCAAGTCACTTTTGTGTTT 727 58 RS0463 TTAGCAGCCACTTTCTCACAATCCA GCAGTTCGCATTTCCAGATCACTAG 726 58 RS0464 GGGGTTCTGCAATGAAGGTGC GCGCTGAAGCATTAGCAGTAGATGT 843 58 RS0465 TTGAAATTGCACACAAGACTAGGGG TTTCTCGGAGTATATGCAATCGGC 698 58 RS0466 AACCTGTCACTATGCGAACCAACAC CATTTCCAGAGCCAGATGTGTGG 533 58 RS0467 ATGAAGAAACTTTGGTCCAGGCTTG TTGCCAACACCAGCTATCGCA 697 58 RS0468 GGTACCACAGCAGATAACGGTTGTG GCACACAAGATAGCTCAAACATCCG 682 58 RS0469 ATGGTTTTCCTGTTGTTGATGAGCC TCATGAGCAATGAAAGCGATAATGC 689 58 RS0470 CTTCAGCCTCAAGTTTGTCACCATG AGCGGAGATCAAGTGAACTTGCTTT 432 58 RS0471 ATTAATTACCATTTGATGGCGAGGG GGATTGGTTATCCAGGTGGTCTCAT 597 58 RS0572 ACAAAAAGCTGAGATACGATTGGCA GGGGATGTTGATGATTGGAAGAAAAA 415 59 RS0573 GCCGGCTTCATGATATCACCAA GAGGGGGCCCAAAAATATCTTTAAT 846 58 RS0574 ATCAATGTTGCCAAATGATGCTGC TGGAGACCTCGATCGAACTAGCTTT 716 58 RS0575 TCGGCAGTATCGTCAGGCACTC GGCCCATGCAAATACACCTGC 712 58 RS0576 TGACGACGAAAGGGAGCCAGT GCAGTATCCCCAGTTCCCCACTAAT 719 58 RS0577 AGAGTTCTTCCTCCTTGTGGGTGTG TTAGTTGCGGATCTTCCATCAGCTA 683 58 RS0578 ACACTCCAACTCTTCCATCGAAGGA TCCACTGTTGATTCAGGCATTGAGT 757 59 RS0579 GCAGACGATCCATCTTGCCCA CGAGGAGAAGAAGGAGACTGAGCAG 696 58 RS0680 TCGCCGGAGAGCATGTTGTC CCTTGGTAACAATCTCCTGGAGGG 708 60 RS0681 TATCAATAATCGTACCTGGGAGCGC CCAGGCGTCAGGTGATTATATTGCT 896 59 RS0682 CAGGGAAACGATGGTGACAACG TGAAGCCCATGCGAATCCATT 706 60 RS0683 AATGAAGCTGAAGTGATGATGCCAC TGGCAGCCATAGTGACTGAAGGATA 709 58 RS0684 AACAAGCATTTTCCCTATGCCACA AGATTTCCCAAGATGGACCAAGTTG 706 58 RS0685 TTTGAATGAAAGCTCGCAGATGATC TGGAACTTCAAGTGAAGGCTGTCAG 743 58 RS0686 TTGAGCTTATGAAACCAACAGCCAA TGAAATGGCCGGGAGTTACAATTA 656 59 RS0687 GAACTTGGGGTTCACCAGAGAGATG GGAAATGACCCCTTGATCATGTGAG 760 58 RS0688 AGGAAGTGAACAACAGCACTCGTGT TTTATTCGAGTTGCGGGAGACGT 865 59 RS0689 CACAACCCTTGCTCCTTCCATG CCTCTCCAGTAAGGTTGCCACAGAT 740 58 RS0690 AGGAATCTCAGGGATATTTGGAGCA TGGAAACCTCACTGCACTTTCTGAA 724 58 RS0691 GCTGCTAGACTAGTTGATGCCATGC GAATTGTGGTTCCTGCTCCATTAGG 765 58 RS0692 GCACGCCGATGTAGTAGAATCCG GGAAGTTCTATGCCGCGTGAATTG 906 58 RS0693 ACTTCCCTTCCTACCCTCGTTTTCA AACCATAGGAGCCAATTCTTGGAAA 702 60 RS07100 GAGCACCTGGCCAAGCAAATCTA CTGTACCGGGAGGTCAGAGGTGA 690 59 RS07101 ACATTGAGACCACTGCTAGGCACAA GCCGATGCTAATAGACTCGCTGAAT 485 58 RS07102 GGCAGTAAAATGCCTCAACACCAA CCATGCAAAACGATTCAAAAGTGG 777 58 RS0794 TGGAATCGAGTTTCCCGTTATTCAT TCGGTATCAATGGCGATGTTTAATG 803 59 RS0795 CGTCATCCAAGCCAGAGACCGT TGGAAGCGTTTCTCCCATGTCTG 711 60 RS0796 CTTCTAGAGAAGCGGTTGCTTGCA GCGATAACCTCATCCTTCTGCTGA 902 59 RS0797 CATTGAGTGACTCGGGATGATATGG GATCTGCTCCCTGCTATTTTGCG 710 60 RS0798 ATACGACCATGAAGCCTGCGTTCT TACTTAGCGGAAACAAGCGGCTTC 700 59 RS0799 TTTGTAACCTGCCAGCATTCATTTC GGGGCAATGTTGTCTTAATGGGTAT 700 60 RS08103 TCAATGCGTCTGGCCTGACAA GAGATATTCCGGTGTGTGACACCAG 682 60 RS08104 TCAGTGACCGTGGATCTGAATGG CATGAGAACTTGCCTGCACAGAATT 684 60 RS08105 TGATTACTACCCAGTCGGCTTCAGC TGCTTCTGCCCTTGTCTATGATTCA 723 58 RS08106 TCAAGTTACCGTAGGCCAGTGTGTG GCCTCTCCCCACAGAATGATCAG 680 58 RS08107 AGGTGTATGTAGGGCCTTTTGTTCG TGGTTTTCAAATATGATGCCCAGAG 696 60 RS08108 AATGATGGGTGTGAATCTATGCAGC GCCCTCTCCTCTCATCTCTCCATTA 815 58 RS08109 CAATCATCCCTAATGGTGAGCAGG ACACCGTGCGCGTCATTGTC 744 60 RS08110 AGGTATGGACGGTTATTGAGGCG CAGCTTCTGGTGTCATCCTCATCAT 683 60 RS08111 CTAGACCTCTGCAACGCCATTCA CATCTGAATAGCGAGGCCGTATCA 774 60 RS08112 TCACTGGCTTCAATGAACTTGCAG TCCAAACACGGCTCTTGAAGTTAAA 745 60 RS09113 GCATGCAGTGCTTTCTAACAAATCC TGCATGTTTGGTCTGTACACCTCAA 848 59 RS09114 TCCTTCTCAAGAACTAGCTGCACCA CAAATACCGGCGCATCTTTGAG 680 58 RS09115 AATGCTAATAGTCCGCCATTTGGAA TTGATCCAGAAGGTGACTGCCAGTA 779 59 RS09116 TTGATAGCCACTTTGGACATGTTGG GCATTATGGCCACACATTGATTGAA 691 58 RS09117 AATTGCGTCTCATCTGTTGGAGCT TTGGGTTGGGACTGGCCTTACTA 727 58 RS10118 GGAGATTTCGTCATCGTGAACGC TGGAGTTGAGAGCTGGGGATTCA 680 58 RS10119 TGTGCATCTGTACACCTCTTGTCCC GACGCCCCTCCTGGTAATACAGAG 773 59 RS10120 ATTGATTTCAACAACAAGCATCCGG TGATGCAATGTCATGTTGGAAGTGA 811 60 RS10121 TTATATTTCTTCACCCGACATGGCC TTGTGTTGCCCTTAAAACCTAAGCA 781 58 RS10122 ATGGAGCAGATGACCATCATCAGC CGTTCACTCGGGAGATTAAAATCCA 773 59 RS10123 GTGGTAGATCAGCGCCAGCATGT CCCTAGCTTGGTCATTGCACAACA 724 59 RS10124 TCAGTGCACTTACTGTCCCCATCTC TTGGTGTTTGAGGAATTGTCTCGC 681 58 RS10125 TTGTAGGTCTACACTGCATCGCGTT TTGGCAACAGAAAATACAGTGGAGG 814 59 RS11126 CGAGCTGTGTTTTGCTTGACATTG CCTTCATGAAAGATGCACGGTGAA 739 58 RS11127 ATCGATCAATGTACTTGCAGATGGG AAAGCCTGACGCATCATCGCTA 758 58 RS11128 GGTAGCATCCCAAGAGAGCTCTTCA GCTCTGGAAAGATCGTCGAATGAAA 665 58 RS11129 TGCATTTGGTCATCTTCAGCTCTTC TCATGCCTATCTAGGTTCCTCCACC 714 59 RS11130 GAGATGACAAGAAGCCCATATTCCC GGCAGATCCCGTCTTTTCTATTCAA 684 58 RS11131 CCTTTATACCACAGGTACCCGGTCA TGGGTTCAAGTCCTTCCATCGAT 680 58 RS11132 CTAACTCATCAATGAAGGCCTGCTG GGACAAGGGCACAGTCTCCAAAA 808 58 RS11133 GTGCTTGCTTGGCAATCACCATT CTCCACAGTTCATCTATCACCCGAA 629 59 RS11134 GCTTCTCGATAGTTTGATGCATTGC GCAACTCGCAGATAGAACCTGGAAT 752 58 RS11135 AGGTAACGGACAGTCCTGATTGACC GATGCATGAGGCCAGTAAGATTGG 684 58 RS11136 TGGCCGAGTCTTTCTCTGCGTATA CGAGATGATGAATGCAAGGACTTGA 693 59 RS11137 AAATCGTGAAGCAGATCATGAACCG GCATTGAGTCGAAAACGGTTCCTTT 704 61.8 RS12138 GACTGACTTTGCCATCATGACCTTG CCAACAGCAAGAGCAAGGCAGATA 685 62 RS12139 TCTTCTTTTGGAACAGTGATGGCTG GCACCGGTTGTGTCTGAAAGAAGTA 755 59 RS12140 CCTTTGTGTGGAAAACGCAAGTAAA GCCATGTAAAAGGCATCCTGTCAAA 688 59 RS12141 ACTCCAGCTTAAAAAAGGGATCCGAG TATTGTAGTTGGCAGGGCCATGTC 755 59 RS12142 GAGGCAATCCATCACCATCAGGT GCGGCTCTTAAAGTTTGATGGACAT 734 58 RS12143 TTTTGAGTTCCCACCAAGAGAATCC GCGAGAAGATATACGGAACGGTGTT 799 59 RS12144 TAATGGAGCTTTCACGGTTCATGC TCGTGGTATGTAACTATGGCCGACA 929 59 RS12145 AGAATGATAATTTGTGTGGCGCTGA TGCACGGTCCTTCTACGTTAATGG 703 58 RS12146 TGATCTGGCAATCAAGTCATGAAGG GGCGATTCCTGTGTACTGTTGGTTT 688 59

Example  3. Genetic and physical mapping

The genotypes of MGRIL were tested using the 146 CAPS markers developed previously. For genetic mapping of this population, 219 DNA markers developed based on PCR were integrated (Non-Patent Document 1) (Table 5). Genetic maps were generated using a total of 365 DNA markers including 146 CAPS markers. The total genetic distance was 1,572 cM and the mean distance between markers was 4.6 cM (Fig. 3). The CAPS marker developed on the basis of the SNP analyzed by the NGS of the present invention is advantageous in that it can be analyzed quickly and efficiently and is composed of PCR-based markers that are easy to analyze. Mapping to the nipponbare genome (build7.0) using the primer sequence of the DNA marker integrated in the genetic map analysis as the standard genome, the physical location of each marker on the rice genome was determined and the physical map was created. In this physical map, the sum of physical distances between markers was 361 Mbp, and the average physical distance per cM was 230 kbp. Based on the results of an integrated analysis of the reported physical and geographical maps of rice, 244 kbp per 1 cM (Non-Patent Document 4), based on the MGRIL hereditary map and physical map created at this time, The distance can be said to be a highly accurate figure (Fig. 2).

The DNA marker information included in the genetic map and physical map of the present invention M arker type no . of markers Marker list InDel ^z 36 R1M7, R1M3, R1M37, R1M47, R2M10, R2M37, R2M50, R3M10, R3M23, R3M37, R4M17, R4M43, R4M50, R5M13, R5M20, R5M30, R6M14, R6M30, R6M44, R7M7, R7M20, R7M37, R8M23, R8M33, R8M46, R9M10, R9M20, R9M30, R9M42, R10M10, R10M17, R10M30, R10M40, R11M17, R11M40, R12M43 RTM ^z 8 RTM4211, RTM10147, RTM3211, RTM5697, RTM3550, RTM10742, RTM9188, RTM5516 STS ^z 88 STS01007, STS01009, STS01017, STS01021, STS01031, STS01039, STS01043, STS02012, STS02017, STS02025, STS02027, STS02030, STS02033, STS02036, STS02038, STS03009, STS03020, STS03025, STS03032, STS03036, STS03040, STS04001, STS04007, STS04009, STS04017, STS04024, STS04037, STS04042, STS05018, STS05020, STS05025, STS05027, STS05028, STS05037, STS05043, STS05045, STS05048, STS06013, STS06019, STS06023, STS06037, STS06040, STS07005, STS07010, STS07015, STS07021, STS07023, STS07025, STS07029, STS07036, STS07040, STS08001, STS08003, STS08005, STS08008, STS08019, STS08021, STS08023, STS08029, STS08033, STS08035, STS08038, STS08043, STS09003, STS09004, STS09007, STS09011, STS09031, STS09033, STS09035, STS09036, STS09048, STS10003, STS10005, STS10010, STS10014, STS10017, STS10037, STS11016, STS11019, STS11025, STS11039, STS12008, STS12011, STS12012, STS12019, STS12023, STS12030 SSR ^z 87 RM110, RM1036, RM11, RM1155, RM1227, RM12368, RM1247, RM1300, RM1370, RM1375, RM167, RM16789, RM17, RM17303, RM17377, RM17960, RM17962, RM1880, RM19218, RM19620, RM201, RM205, RM20882, RM212, RM2136, RM214, RM224, RM226, RM22608, RM22630, RM22694, RM234, RM23736, RM23779, RM240, RM242, RM246, RM247, RM253, RM25366, RM257, RM26062, RM276, RM277, RM27970, RM28400, RM370, RM3199, RM3252, RM332, RM3394, RM3472, RM3481, RM349, RM3664, RM3765, RM401, RM408, RM420, RM4355, RM44, RM4771, RM5055, RM5349, RM536, RM5526, RM5608, RM5633, RM5753, RM5807, RM5814, RM5907, RM6367, RM6467, RM6775, RM6840, RM6841, RM6842, RM7000, RM7389, RM7492, RM7631 SNP 146 Wherein R is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, arylalkynyl, arylalkynyl, arylalkynyl, arylalkynyl, arylalkynyl, arylalkynyl, Wherein the at least one amino acid is selected from the group consisting of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 10, 11, 12, 13, 14, 15, 16, 19, The present invention also relates to the use of a polynucleotide encoding a polynucleotide selected from the group consisting of: RS0576, RS0577, RS0578, RS0579, RS0680, RS0681, RS0682, RS0683, RS0684, RS0685, RS0686, RS0687, RS0688, RS0689, RS0690, RS0691, RS0692, RS0693, RS0794, RS0795, RS0796, RS0797, RS0798, RS07101, RS07102, RS08103, RS08104, RS08105, RS08106, RS08107, RS08108, RS08109, RS08110, RS08111, RS08112, RS09113, RS09114, RS09115, RS09116, RS09117, RS10118, RS10119, RS10120, RS10121, RS10122, RS12144, RS12145, RS12144, RS12145, RS12144, RS12145, RS12144, RS12145, RS12144, RS12145, RS12144, RS12144, RS12144, RS12144, RS12145, RS11125, RS11126, RS11127, RS11128, RS11129, RS11130, RS11131, RS11132, RS11133, RS11134, RS11135,

^z Ji et al. (2012)

Example  4. MGRIL  Phenotypic correlations and QTL  analysis

(I1D), 2 section thickness (I2D), 3 section thickness (I3D), 4 section thickness (I4D), liver (CL), and shoot (PL) phenotype for Milyang 23, (Fig. 4). Variation distributions of irradiated traits showed continuous normal distribution and showed transcendental separation beyond the range of the sample. As a result of correlation analysis between six traits, it was found that there was a significant correlation between length and length of four stems, and length of length of stem increased with thickening of stems. (Table 6). It was confirmed that the liver and stem thickness had a low correlation or no correlation.

There are no reports on stem diameter QTL for the recombinant heritable populations of Milyang 23 and Rice. In this study, a total of 4 stem-related QTLs were newly discovered (Table 7). Two of the QTLs related to the traits in the first section were named qI1D1 and qI1D5, respectively. qI1D1 was found between RS0124-RS0125 on chromosome 1, and the one-fold phenotypic variation of this QTL was 5.02%. In alleles of representative rice, the 1st section thickness increased by 0.07 cm and the LOD value was 3.26 (Table 7). qI1D5 was found between RM6841-RS0578 on chromosome 5, and the variant of the first-fold thickness of this QTL was 8.99%. The allele of Milyang 23 increased 0.10 cm in the thickness of the first section, and the LOD value was 6.09, the highest value of newly found QTL (Table 7). In addition, one QTL was identified for the traits related to the thickness of Section 3, which was named qI3D1. This QTL was found between STS01039-RS0124 on chromosome 3, and the phenotypic variation of the 3-fold was 6.53%. The LOD value of this QTL was 3.17, and the allele of the representative rice increased the 3-fold thickness by 0.18 cm (Table 7). One QTL for the 4-fold thickness trait was searched and named qI4D1. This QTL was found between STS01039-RS0124 on chromosome 1, and the phenotype of the 4-fold phenotype was 9.97%. The LOD value of this QTL was 4.61, and the allele of the seed rice increased the 4-fold thickness by 0.25 cm (Table 7). qI1D1, qI3D1, qI4D1 were found in similar regions of chromosome 1. In particular, QTLs related to curves 3 and 4 were searched between the same markers. However, stem size measurement and QTL analysis were independent, and several traits may be dominated by one gene, but different QTLs were arbitrarily named because they may be dominated by different genes. Further studies are required to determine whether each trait is actually a different gene.

This QTL analysis is expected to be the basis for genetic tagging by identifying the linkage between useful traits and DNA markers, and for carrying out substantive breeding using these markers.

Correlation between six traits measured in MGRIL subjects Trait I1D I2D I3D I4D CL PL I1D ^z 0.8441 ^** 0.6546 ^** 0.6025 ^** 0.0557 (ns ^y ) 0.5298 ^** I2D 0.8578 ^** 0.7987 ^** 0.1154 (ns) 0.5264 ^** I3D 0.9347 ^** 0.3184 ^** 0.4964 ^** I4D 0.3602 ^** 0.4688 ^** CL 0.2343 ^**

^** p < 0.01

^z I1D = first internode diameter; I2D = second internode diameter; I3D = third internode diameter; I4D = fourth internode diameter; CL = culm length; PL = Panicle length.

^y ns, not significant.

Characteristics of QTL markers detected by 6 traits of MGRIL Traits No. QTL name ^z Chr ^y Position LOD ^w Additive R ² Interval marker Reference (cM) ^x effect ^v (%) ^u First
Internode One One 27.73 12.56 0.15 21.19 RS014 , RM1 sdm1 (Kashiwagi et al. 2008) 2 qI1D1 One 196.62 3.26 -0.07 5.02 RS0124 , RS0125 3 qI1D5 5 84.14 6.09 0.10 8.99 RM6841 , RS0578 4 6 119.17 3.75 0.08 6.06 RS0693 , RM5814 Kashiwagi & Ishimaru (2004) Second
Internode 5 One 27.73 5.86 0.17 11.11 RS014 , RM1 sdm1 (Kashiwagi et al. 2008) 6 6 110.87 6.10 0.18 12.18 RS0691 , RM1370 Kashiwagi & Ishimaru (2004) 7 7 57.00 3.19 0.13 6.31 STS07015 , RS0798 sdm7 (Kashiwagi et al. 2008) Third
Internode 8 qI3D1 One 192.10 3.17 -0.18 6.53 STS01039 , RS0124 9 4 74.84 4.12 -0.20 7.76 RS0462 , RS0463 qCD -4 (Wang et al. 2011) 10 6 113.39 6.45 0.26 12.94 RM1370 , RS0692 Kashiwagi & Ishimaru (2004) Fourth
Internode 11 qI4D1 One 193.10 4.61 -0.25 9.97 STS01039 , RS0124 12 6 107.87 7.47 0.34 19.25 RS0691 , RM1370 Kashiwagi & Ishimaru (2004) 13 12 20.24 3.22 0.19 5.95 RS12140 , STS12019 sdm12 (Kashiwagi et al. 2008), Culm
length 14 One 171.43 31.14 -9.99 58.23 RS0119 , RS0120 sd- 1 ( Ashikari et al. 2002,
Cho et al. 1994,
Monna et al. 2002,
Sasaki et al. 2002,
Spielmeyer et al. 2002) 15 qCL5 5 41.08 3.51 2.72 4.24 R5M13 , RS0573 16 8 12.16 5.37 3.33 6.44 STS08005 , STS08008 pl8  ( Xiao meat get . 1995),
ph8  ( Xiong meat get . 1999) 17 qCL12 12 94.2 3.09 2.47 3.59 RS12143 , RM1300 Panicle
length 18 One 134.68 3.32 0.64 6.59 R1M37 , RS0115 pl1 (Xiong et al. 1999),
pl1 .1 (Septiningsih meat al . 2003) 19 3 41.08 5.44 0.84 11.03 R3M10 , RTM5697 Pl3. 1 (Septiningsih et al. 2003),
pl3b (Zhuang et al. 1997)

^z : QTLs were newly detected and tentatively named.

^y : The number of chromosome.

^x : Position of QTL from the top of each chromosome.

^w : The logarithm of the ratio of two likelihoods.

^v : Positive and negative values indicate the additive effect contributed by the alleles of Milyang23 and Gihobyeo, respectively.

^u : Percentage of variance explained by each QTL.

<110> REPUBLIC OF KOREA <120> QTL ANALYSIS OF STEM DIAMETER AND CAPS MARKER THEREFOR <130> P14R12D1002 <160> 292 <170> Kopatentin 2.0 <210> 1 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS011 FORWARD PRIMER <400> 1 tccagaagtc aggataggag tggca 25 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS011 REVERSE PRIMER <400> 2 tggaagaacc acaactgcat catg 24 <210> 3 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0110 FORWARD PRIMER <400> 3 tgaaaccctt cttcaggaaa ccagc 25 <210> 4 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0110 REVERSE PRIMER <400> 4 cattgtgcag ccaggaaaag gataa 25 <210> 5 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0111 FORWARD PRIMER <400> 5 cagtgcagcg atctttatca gcttc 25 <210> 6 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0111 REVERSE PRIMER <400> 6 tcgatcctcg agtccatgca tg 22 <210> 7 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0112 FORWARD PRIMER <400> 7 tcctgctttt atgttggctg tcact 25 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0112 REVERSE PRIMER <400> 8 ggctgctgct tcaaaattcc cttt 24 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0113 FORWARD PRIMER <400> 9 tgcattttgg agcattgtag acgc 24 <210> 10 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0113 REVERSE PRIMER <400> 10 tgcaagcaca gaagtgggca gt 22 <210> 11 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0114 FORWARD PRIMER <400> 11 cacacgcagc gcagaaacta aaa 23 <210> 12 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0114 REVERSE PRIMER <400> 12 ccatcgaaaa tccactgcaa catt 24 <210> 13 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0115 FORWARD PRIMER <400> 13 aatggtcttg gtgacgaagc aactg 25 <210> 14 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0115 REVERSE PRIMER <400> 14 cgaataggtg accgagcagc act 23 <210> 15 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0116 FORWARD PRIMER <400> 15 tgcagcgcca tcatcatgag c 21 <210> 16 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0116 REVERSE PRIMER <400> 16 agttgaggaa gctgagcacg gc 22 <210> 17 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0117 FORWARD PRIMER <400> 17 agggattaat tgattcacac agccc 25 <210> 18 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0117 REVERSE PRIMER <400> 18 cccagttgat gcaactcata tccct 25 <210> 19 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0118 FORWARD PRIMER <400> 19 atatgatgtc acccaaagtt gccct 25 <210> 20 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0118 REVERSE PRIMER <400> 20 gtgttccgtg ttcttgattt cgtgt 25 <210> 21 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0119 FORWARD PRIMER <400> 21 gggacattgg cttgacacat aagtg 25 <210> 22 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0119 REVERSE PRIMER <400> 22 acgaacactc tggcaaggac ca 22 <210> 23 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS012 FORWARD PRIMER <400> 23 ttgttcttgt aaaggcgctt cactg 25 <210> 24 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS012 REVERSE PRIMER <400> 24 actgtccttc gtttcaacag ggg 23 <210> 25 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0120 FORWARD PRIMER <400> 25 acacgtgctg ccaaatattg cg 22 <210> 26 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0120 REVERSE PRIMER <400> 26 ttttgcagtc ctgcagacca tttaa 25 <210> 27 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0121 FORWARD PRIMER <400> 27 tttaccaagc aggggatcag aaatg 25 <210> 28 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0121 REVERSE PRIMER <400> 28 tgcattgcat tacctgtgtc ctttt 25 <210> 29 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0122 FORWARD PRIMER <400> 29 ggcagaacag gagcattagc aagc 24 <210> 30 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0122 REVERSE PRIMER <400> 30 cctggactgg actggagtat aggga 25 <210> 31 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0123 FORWARD PRIMER <400> 31 ctgcaactca atggctccta ggtg 24 <210> 32 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0123 REVERSE PRIMER <400> 32 gctggcagag ctttatgtaa tggga 25 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0124 FORWARD PRIMER <400> 33 caggaggaaa acacaccgga tgg 23 <210> 34 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0124 REVERSE PRIMER <400> 34 ccgtccactg taagaactga atgcc 25 <210> 35 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0125 FORWARD PRIMER <400> 35 atgcggagga aatgccaaac a 21 <210> 36 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0125 REVERSE PRIMER <400> 36 ggatcgatgc gtgaaatatt gaaga 25 <210> 37 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS013 FORWARD PRIMER <400> 37 atcagggagt ttgcaatcaa aatgg 25 <210> 38 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS013 REVERSE PRIMER <400> 38 cccttgtggt atctgtccag tcaaa 25 <210> 39 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS014 FORWARD PRIMER <400> 39 tcagaagatt gtacctttgg ctccc 25 <210> 40 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS014 REVERSE PRIMER <400> 40 gggaagttcc tgctgaagag aggta 25 <210> 41 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS015 FORWARD PRIMER <400> 41 tgtgttcgtg ataggatgca cagg 24 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS015 REVERSE PRIMER <400> 42 cgcacctccc ctccatgtaa a 21 <210> 43 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS016 FORWARD PRIMER <400> 43 atcccatcca catcctttga atcc 24 <210> 44 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS016 REVERSE PRIMER <400> 44 cgaggtcaag agaatctggg agtct 25 <210> 45 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS017 FORWARD PRIMER <400> 45 tcagcaaatg gagttggcta aacaa 25 <210> 46 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS017 REVERSE PRIMER <400> 46 agtgatggag aggccaaaat gtctt 25 <210> 47 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS018 FORWARD PRIMER <400> 47 tgaaggggct gatcgagctc ca 22 <210> 48 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS018 REVERSE PRIMER <400> 48 tggatccggc gacgactact tatt 24 <210> 49 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS019 FORWARD PRIMER <400> 49 acttcatggg gacgagaagg tacg 24 <210> 50 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS019 REVERSE PRIMER <400> 50 cataggtagg aggcacacat ccgt 24 <210> 51 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0226 FORWARD PRIMER <400> 51 tgaagctgct tctaaatcac catcg 25 <210> 52 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0226 REVERSE PRIMER <400> 52 ctcacaggac caataccaag atgga 25 <210> 53 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0227 FORWARD PRIMER <400> 53 atctggctca agactgtccc tgatc 25 <210> 54 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0227 REVERSE PRIMER <400> 54 ggcaccactg catcatacgt ctact 25 <210> 55 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0228 FORWARD PRIMER <400> 55 gagatgttta gcgggaagag accaa 25 <210> 56 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0228 REVERSE PRIMER <400> 56 ggatggaatc ctgcgggtag ttaa 24 <210> 57 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0229 FORWARD PRIMER <400> 57 gaagttgcaa agatggagga actcg 25 <210> 58 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0229 REVERSE PRIMER <400> 58 tgcatggcca gtgaggatgg t 21 <210> 59 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0230 FORWARD PRIMER <400> 59 aaataaaaga caaacgggga gcaca 25 <210> 60 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0230 REVERSE PRIMER <400> 60 caaaaccgaa tcgcccctat aaatt 25 <210> 61 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0231 FORWARD PRIMER <400> 61 ctgctggcca gcgttctaca ag 22 <210> 62 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0231 REVERSE PRIMER <400> 62 ccaaccgcaa atcagcctac aa 22 <210> 63 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0232 FORWARD PRIMER <400> 63 ttgggctgaa gcaaagagcg a 21 <210> 64 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0232 REVERSE PRIMER <400> 64 cgtctgctgg aagacaaaat gaagg 25 <210> 65 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0233 FORWARD PRIMER <400> 65 tgtcatctgg aagtatggag gctca 25 <210> 66 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0233 REVERSE PRIMER <400> 66 tgttgctcta gctggggcat taaat 25 <210> 67 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0234 FORWARD PRIMER <400> 67 aaaatggtgc acgaagcaat tgatg 25 <210> 68 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0234 REVERSE PRIMER <400> 68 cccctgctga aaaaggaaaa tgag 24 <210> 69 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0235 FORWARD PRIMER <400> 69 agcatcttaa taggtttgcg gatgg 25 <210> 70 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0235 REVERSE PRIMER <400> 70 caaacacggg agctctccat acatt 25 <210> 71 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0236 FORWARD PRIMER <400> 71 tacctgaatc acaaccttct gtgcc 25 <210> 72 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0236 REVERSE PRIMER <400> 72 cctttggctg caatatgtct tgttg 25 <210> 73 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0237 FORWARD PRIMER <400> 73 ctggcattga tccgcattga ga 22 <210> 74 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0237 REVERSE PRIMER <400> 74 tcgtgtgatg acagttgagc aaaca 25 <210> 75 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0238 FORWARD PRIMER <400> 75 cgctgacata gcaaatctga tctcc 25 <210> 76 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0238 REVERSE PRIMER <400> 76 ccggtcgaga gaagctctgt agaag 25 <210> 77 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0239 FORWARD PRIMER <400> 77 agaaaatgcc tgcagttgtg ttgtc 25 <210> 78 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0239 REVERSE PRIMER <400> 78 ttccaaacaa tctgcagcca gc 22 <210> 79 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0240 FORWARD PRIMER <400> 79 ggaagtcatc ctgatccagc ttgtg 25 <210> 80 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0240 REVERSE PRIMER <400> 80 acgcctccag attcaagcaa gag 23 <210> 81 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0241 FORWARD PRIMER <400> 81 gccaagtggc acattcccaa c 21 <210> 82 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0241 REVERSE PRIMER <400> 82 agccggaagg atacacgaat cag 23 <210> 83 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0242 FORWARD PRIMER <400> 83 tgttaatgag gttgccatct tgtcg 25 <210> 84 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0242 REVERSE PRIMER <400> 84 ccattacaga aatgggttgg cactt 25 <210> 85 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0243 FORWARD PRIMER <400> 85 ttgacagtac atgctgggtt aggga 25 <210> 86 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0243 REVERSE PRIMER <400> 86 gcagcttttc attctgagcg ca 22 <210> 87 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0244 FORWARD PRIMER <400> 87 ttccagagta gcagcatctt gtgca 25 <210> 88 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0244 REVERSE PRIMER <400> 88 tggttttagt ggaggtgatt cgttg 25 <210> 89 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0245 FORWARD PRIMER <400> 89 tgtacggcta cacagctgaa attcc 25 <210> 90 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0245 REVERSE PRIMER <400> 90 accattttcc gaactgcttt ctttc 25 <210> 91 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0246 FORWARD PRIMER <400> 91 acgattattg ccatgacaag atcga 25 <210> 92 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0246 REVERSE PRIMER <400> 92 ggtttgtgtt ctcccactct ccaa 24 <210> 93 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0347 FORWARD PRIMER <400> 93 ttcgaaaata aaaaccctgc tccag 25 <210> 94 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0347 REVERSE PRIMER <400> 94 cctcaaaatg cctttccaaa ttcag 25 <210> 95 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0348 FORWARD PRIMER <400> 95 ttcactttcc tcctcacttt cgtca 25 <210> 96 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0348 REVERSE PRIMER <400> 96 ggcagtgcat tttggacaca cagta 25 <210> 97 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0349 FORWARD PRIMER <400> 97 tggcttgttt tgtgcgaagg g 21 <210> 98 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0349 REVERSE PRIMER <400> 98 cccattgacc aagtctttca caaga 25 <210> 99 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0350 FORWARD PRIMER <400> 99 tctcaatctt aaccccactc ttcgg 25 <210> 100 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0350 REVERSE PRIMER <400> 100 tgaccactgt ctgacatggg ctc 23 <210> 101 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0351 FORWARD PRIMER <400> 101 tcaaaattct gtgaagaccg atgga 25 <210> 102 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0351 REVERSE PRIMER <400> 102 caacaatttg ccaacctcat tgc 23 <210> 103 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0352 FORWARD PRIMER <400> 103 tttcgcaata caagtaggac tgcca 25 <210> 104 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0352 REVERSE PRIMER <400> 104 gtggtgtcgc tcaaacaggt ttg 23 <210> 105 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0353 FORWARD PRIMER <400> 105 atctagcagt cgattgttgg cattg 25 <210> 106 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0353 REVERSE PRIMER <400> 106 tggtgctgta attccatttc catca 25 <210> 107 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0354 FORWARD PRIMER <400> 107 ttgctgcctc cagttggaag tct 23 <210> 108 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0354 REVERSE PRIMER <400> 108 cccaacagca agcatacaca cactc 25 <210> 109 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0355 FORWARD PRIMER <400> 109 tgaccagcat cagcatatca aaaca 25 <210> 110 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0355 REVERSE PRIMER <400> 110 gcagcaaggt catgatgacc atatt 25 <210> 111 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0356 FORWARD PRIMER <400> 111 atgtaatcca tgccccttat tttgc 25 <210> 112 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0356 REVERSE PRIMER <400> 112 tcagagcttc tggaggtgag gaatt 25 <210> 113 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0457 FORWARD PRIMER <400> 113 catcattgtg caggtatggg agaaa 25 <210> 114 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0457 REVERSE PRIMER <400> 114 cccgcttaaa tcgtcccttt tgta 24 <210> 115 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0458 FORWARD PRIMER <400> 115 gatgatgaaa atgacactgc ttggg 25 <210> 116 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0458 REVERSE PRIMER <400> 116 gcaaagtaaa cagggcaagc aaaa 24 <210> 117 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0459 FORWARD PRIMER <400> 117 agcaattttt cgcctgcatt cc 22 <210> 118 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0459 REVERSE PRIMER <400> 118 tggaacatgc cattcattga cagag 25 <210> 119 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0460 FORWARD PRIMER <400> 119 ataggaaaat tcacctcgac agccg 25 <210> 120 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0460 REVERSE PRIMER <400> 120 tggcagctca acatgactgg c 21 <210> 121 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0461 FORWARD PRIMER <400> 121 cctggaacaa ccaaacactg acttg 25 <210> 122 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0461 REVERSE PRIMER <400> 122 cgccgtcgca attaaaatgt gc 22 <210> 123 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0462 FORWARD PRIMER <400> 123 ggaactggct acaggaaaga cttgg 25 <210> 124 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0462 REVERSE PRIMER <400> 124 agccctccaa gtcacttttg tgttt 25 <210> 125 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0463 FORWARD PRIMER <400> 125 ttagcagcca ctttctcaca atcca 25 <210> 126 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0463 REVERSE PRIMER <400> 126 gcagttcgca tttccagatc actag 25 <210> 127 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0464 FORWARD PRIMER <400> 127 ggggttctgc aatgaaggtg c 21 <210> 128 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0464 REVERSE PRIMER <400> 128 gcgctgaagc attagcagta gatgt 25 <210> 129 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0465 FORWARD PRIMER <400> 129 ttgaaattgc acacaagact agggg 25 <210> 130 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0465 REVERSE PRIMER <400> 130 tttctcggag tatatgcaat cggc 24 <210> 131 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0466 FORWARD PRIMER <400> 131 aacctgtcac tatgcgaacc aacac 25 <210> 132 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0466 REVERSE PRIMER <400> 132 catttccaga gccagatgtg tgg 23 <210> 133 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0467 FORWARD PRIMER <400> 133 atgaagaaac tttggtccag gcttg 25 <210> 134 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0467 REVERSE PRIMER <400> 134 ttgccaacac cagctatcgc a 21 <210> 135 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0468 FORWARD PRIMER <400> 135 ggtaccacag cagataacgg ttgtg 25 <210> 136 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0468 REVERSE PRIMER <400> 136 gcacacaaga tagctcaaac atccg 25 <210> 137 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0469 FORWARD PRIMER <400> 137 atggttttcc tgttgttgat gagcc 25 <210> 138 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0469 REVERSE PRIMER <400> 138 tcatgagcaa tgaaagcgat aatgc 25 <210> 139 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0470 FORWARD PRIMER <400> 139 cttcagcctc aagtttgtca ccatg 25 <210> 140 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0470 REVERSE PRIMER <400> 140 agcggagatc aagtgaactt gcttt 25 <210> 141 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0471 FORWARD PRIMER <400> 141 attaattacc atttgatggc gaggg 25 <210> 142 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0471 REVERSE PRIMER <400> 142 ggattggtta tccaggtggt ctcat 25 <210> 143 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0572 FORWARD PRIMER <400> 143 acaaaaagct gagatacgat tggca 25 <210> 144 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0572 REVERSE PRIMER <400> 144 ggggatgttg atgattggaa gaaaa 25 <210> 145 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0573 FORWARD PRIMER <400> 145 gccggcttca tgatatcacc aa 22 <210> 146 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0573 REVERSE PRIMER <400> 146 gagggggccc aaaaatatct ttaat 25 <210> 147 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0574 FORWARD PRIMER <400> 147 atcaatgttg ccaaatgatg ctgc 24 <210> 148 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0574 REVERSE PRIMER <400> 148 tggagacctc gatcgaacta gcttt 25 <210> 149 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0575 FORWARD PRIMER <400> 149 tcggcagtat cgtcaggcac tc 22 <210> 150 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0575 REVERSE PRIMER <400> 150 ggcccatgca aatacacctg c 21 <210> 151 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0576 FORWARD PRIMER <400> 151 tgacgacgaa agggagccag t 21 <210> 152 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0576 REVERSE PRIMER <400> 152 gcagtatccc cagttcccca ctaat 25 <210> 153 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0577 FORWARD PRIMER <400> 153 agagttcttc ctccttgtgg gtgtg 25 <210> 154 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0577 REVERSE PRIMER <400> 154 ttagttgcgg atcttccatc agcta 25 <210> 155 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0578 FORWARD PRIMER <400> 155 acactccaac tcttccatcg aagga 25 <210> 156 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0578 REVERSE PRIMER <400> 156 tccactgttg attcaggcat tgagt 25 <210> 157 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0579 FORWARD PRIMER <400> 157 gcagacgatc catcttgccc a 21 <210> 158 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0579 REVERSE PRIMER <400> 158 cgaggagaag aaggagactg agcag 25 <210> 159 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> RS0680 FORWARD PRIMER <400> 159 tcgccggaga gcatgttgtc 20 <210> 160 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0680 REVERSE PRIMER <400> 160 ccttggtaac aatctcctgg aggg 24 <210> 161 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0681 FORWARD PRIMER <400> 161 tatcaataat cgtacctggg agcgc 25 <210> 162 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0681 REVERSE PRIMER <400> 162 ccaggcgtca ggtgattata ttgct 25 <210> 163 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0682 FORWARD PRIMER <400> 163 cagggaaacg atggtgacaa cg 22 <210> 164 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS0682 REVERSE PRIMER <400> 164 tgaagcccat gcgaatccat t 21 <210> 165 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0683 FORWARD PRIMER <400> 165 aatgaagctg aagtgatgat gccac 25 <210> 166 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0683 REVERSE PRIMER <400> 166 tggcagccat agtgactgaa ggata 25 <210> 167 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0684 FORWARD PRIMER <400> 167 aacaagcatt ttccctatgc caca 24 <210> 168 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0684 REVERSE PRIMER <400> 168 agatttccca agatggacca agttg 25 <210> 169 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0685 FORWARD PRIMER <400> 169 tttgaatgaa agctcgcaga tgatc 25 <210> 170 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0685 REVERSE PRIMER <400> 170 tggaacttca agtgaaggct gtcag 25 <210> 171 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0686 FORWARD PRIMER <400> 171 ttgagcttat gaaaccaaca gccaa 25 <210> 172 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0686 REVERSE PRIMER <400> 172 tgaaatggcc gggagttaca atta 24 <210> 173 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0687 FORWARD PRIMER <400> 173 gaacttgggg ttcaccagag agatg 25 <210> 174 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0687 REVERSE PRIMER <400> 174 ggaaatgacc ccttgatcat gtgag 25 <210> 175 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0688 FORWARD PRIMER <400> 175 aggaagtgaa caacagcact cgtgt 25 <210> 176 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0688 REVERSE PRIMER <400> 176 tttattcgag ttgcgggaga cgt 23 <210> 177 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0689 FORWARD PRIMER <400> 177 cacaaccctt gctccttcca tg 22 <210> 178 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0689 REVERSE PRIMER <400> 178 cctctccagt aaggttgcca cagat 25 <210> 179 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0690 FORWARD PRIMER <400> 179 aggaatctca gggatatttg gagca 25 <210> 180 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0690 REVERSE PRIMER <400> 180 tggaaacctc actgcacttt ctgaa 25 <210> 181 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0691 FORWARD PRIMER <400> 181 gctgctagac tagttgatgc catgc 25 <210> 182 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0691 REVERSE PRIMER <400> 182 gaattgtggt tcctgctcca ttagg 25 <210> 183 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0692 FORWARD PRIMER <400> 183 gcacgccgat gtagtagaat ccg 23 <210> 184 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0692 REVERSE PRIMER <400> 184 ggaagttcta tgccgcgtga attg 24 <210> 185 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0693 FORWARD PRIMER <400> 185 acttcccttc ctaccctcgt tttca 25 <210> 186 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0693 REVERSE PRIMER <400> 186 aaccatagga gccaattctt ggaaa 25 <210> 187 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS07100 FORWARD PRIMER <400> 187 gagcacctgg ccaagcaaat cta 23 <210> 188 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS07100 REVERSE PRIMER <400> 188 ctgtaccggg aggtcagagg tga 23 <210> 189 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS07101 FORWARD PRIMER <400> 189 acattgagac cactgctagg cacaa 25 <210> 190 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS07101 REVERSE PRIMER <400> 190 gccgatgcta atagactcgc tgaat 25 <210> 191 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS07102 FORWARD PRIMER <400> 191 ggcagtaaaa tgcctcaaca ccaa 24 <210> 192 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS07102 REVERSE PRIMER <400> 192 ccatgcaaaa cgattcaaaa gtgg 24 <210> 193 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0794 FORWARD PRIMER <400> 193 tggaatcgag tttcccgtta ttcat 25 <210> 194 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0794 REVERSE PRIMER <400> 194 tcggtatcaa tggcgatgtt taatg 25 <210> 195 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS0795 FORWARD PRIMER <400> 195 cgtcatccaa gccagagacc gt 22 <210> 196 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0795 REVERSE PRIMER <400> 196 tggaagcgtt tctcccatgt ctg 23 <210> 197 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0796 FORWARD PRIMER <400> 197 cttctagaga agcggttgct tgca 24 <210> 198 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0796 REVERSE PRIMER <400> 198 gcgataacct catccttctg ctga 24 <210> 199 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0797 FORWARD PRIMER <400> 199 cattgagtga ctcgggatga tatgg 25 <210> 200 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS0797 REVERSE PRIMER <400> 200 gatctgctcc ctgctatttt gcg 23 <210> 201 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0798 FORWARD PRIMER <400> 201 atacgaccat gaagcctgcg ttct 24 <210> 202 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS0798 REVERSE PRIMER <400> 202 tacttagcgg aaacaagcgg cttc 24 <210> 203 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0799 FORWARD PRIMER <400> 203 tttgtaacct gccagcattc atttc 25 <210> 204 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS0799 REVERSE PRIMER <400> 204 ggggcaatgt tgtcttaatg ggtat 25 <210> 205 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RS08103 FORWARD PRIMER <400> 205 tcaatgcgtc tggcctgaca a 21 <210> 206 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08103 REVERSE PRIMER <400> 206 gagatattcc ggtgtgtgac accag 25 <210> 207 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS08104 FORWARD PRIMER <400> 207 tcagtgaccg tggatctgaa tgg 23 <210> 208 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08104 REVERSE PRIMER <400> 208 catgagaact tgcctgcaca gaatt 25 <210> 209 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08105 FORWARD PRIMER <400> 209 tgattactac ccagtcggct tcagc 25 <210> 210 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08105 REVERSE PRIMER <400> 210 tgcttctgcc cttgtctatg attca 25 <210> 211 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08106 FORWARD PRIMER <400> 211 tcaagttacc gtaggccagt gtgtg 25 <210> 212 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS08106 REVERSE PRIMER <400> 212 gcctctcccc acagaatgat cag 23 <210> 213 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08107 FORWARD PRIMER <400> 213 aggtgtatgt agggcctttt gttcg 25 <210> 214 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08107 REVERSE PRIMER <400> 214 tggttttcaa atatgatgcc cagag 25 <210> 215 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08108 FORWARD PRIMER <400> 215 aatgatgggt gtgaatctat gcagc 25 <210> 216 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08108 REVERSE PRIMER <400> 216 gccctctcct ctcatctctc catta 25 <210> 217 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS08109 FORWARD PRIMER <400> 217 caatcatccc taatggtgag cagg 24 <210> 218 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> RS08109 REVERSE PRIMER <400> 218 acaccgtgcg cgtcattgtc 20 <210> 219 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS08110 FORWARD PRIMER <400> 219 aggtatggac ggttattgag gcg 23 <210> 220 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08110 REVERSE PRIMER <400> 220 cagcttctgg tgtcatcctc atcat 25 <210> 221 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS08111 FORWARD PRIMER <400> 221 ctagacctct gcaacgccat tca 23 <210> 222 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS08111 REVERSE PRIMER <400> 222 catctgaata gcgaggccgt atca 24 <210> 223 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS08112 FORWARD PRIMER <400> 223 tcactggctt caatgaactt gcag 24 <210> 224 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS08112 REVERSE PRIMER <400> 224 tccaaacacg gctcttgaag ttaaa 25 <210> 225 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09113 FORWARD PRIMER <400> 225 gcatgcagtg ctttctaaca aatcc 25 <210> 226 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09113 REVERSE PRIMER <400> 226 tgcatgtttg gtctgtacac ctcaa 25 <210> 227 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09114 FORWARD PRIMER <400> 227 tccttctcaa gaactagctg cacca 25 <210> 228 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS09114 REVERSE PRIMER <400> 228 caaataccgg cgcatctttg ag 22 <210> 229 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09115 FORWARD PRIMER <400> 229 aatgctaata gtccgccatt tggaa 25 <210> 230 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09115 REVERSE PRIMER <400> 230 ttgatccaga aggtgactgc cagta 25 <210> 231 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09116 FORWARD PRIMER <400> 231 ttgatagcca ctttggacat gttgg 25 <210> 232 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS09116 REVERSE PRIMER <400> 232 gcattatggc cacacattga ttgaa 25 <210> 233 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS09117 FORWARD PRIMER <400> 233 aattgcgtct catctgttgg agct 24 <210> 234 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS09117 REVERSE PRIMER <400> 234 ttgggttggg actggcctta cta 23 <210> 235 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS10118 FORWARD PRIMER <400> 235 ggagatttcg tcatcgtgaa cgc 23 <210> 236 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS10118 REVERSE PRIMER <400> 236 tggagttgag agctggggat tca 23 <210> 237 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10119 FORWARD PRIMER <400> 237 tgtgcatctg tacacctctt gtccc 25 <210> 238 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS10119 REVERSE PRIMER <400> 238 gacgcccctc ctggtaatac agag 24 <210> 239 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10120 FORWARD PRIMER <400> 239 attgatttca acaacaagca tccgg 25 <210> 240 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10120 REVERSE PRIMER <400> 240 tgatgcaatg tcatgttgga agtga 25 <210> 241 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10121 FORWARD PRIMER <400> 241 ttatatttct tcacccgaca tggcc 25 <210> 242 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10121 REVERSE PRIMER <400> 242 ttgtgttgcc cttaaaacct aagca 25 <210> 243 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS10122 FORWARD PRIMER <400> 243 atggagcaga tgaccatcat cagc 24 <210> 244 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10122 REVERSE PRIMER <400> 244 cgttcactcg ggagattaaa atcca 25 <210> 245 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS10123 FORWARD PRIMER <400> 245 gtggtagatc agcgccagca tgt 23 <210> 246 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS10123 REVERSE PRIMER <400> 246 ccctagcttg gtcattgcac aaca 24 <210> 247 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10124 FORWARD PRIMER <400> 247 tcagtgcact tactgtcccc atctc 25 <210> 248 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS10124 REVERSE PRIMER <400> 248 ttggtgtttg aggaattgtc tcgc 24 <210> 249 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10125 FORWARD PRIMER <400> 249 ttgtaggtct acactgcatc gcgtt 25 <210> 250 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS10125 REVERSE PRIMER <400> 250 ttggcaacag aaaatacagt ggagg 25 <210> 251 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS11126 FORWARD PRIMER <400> 251 cgagctgtgt tttgcttgac attg 24 <210> 252 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS11126 REVERSE PRIMER <400> 252 ccttcatgaa agatgcacgg tgaa 24 <210> 253 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11127 FORWARD PRIMER <400> 253 atcgatcaat gtacttgcag atggg 25 <210> 254 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> RS11127 REVERSE PRIMER <400> 254 aaagcctgac gcatcatcgc ta 22 <210> 255 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11128 FORWARD PRIMER <400> 255 ggtagcatcc caagagagct cttca 25 <210> 256 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11128 REVERSE PRIMER <400> 256 gctctggaaa gatcgtcgaa tgaaa 25 <210> 257 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11129 FORWARD PRIMER <400> 257 tgcatttggt catcttcagc tcttc 25 <210> 258 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11129 REVERSE PRIMER <400> 258 tcatgcctat ctaggttcct ccacc 25 <210> 259 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11130 FORWARD PRIMER <400> 259 gagatgacaa gaagcccata ttccc 25 <210> 260 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11130 REVERSE PRIMER <400> 260 ggcagatccc gtcttttcta ttcaa 25 <210> 261 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11131 FORWARD PRIMER <400> 261 cctttatacc acaggtaccc ggtca 25 <210> 262 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS11131 REVERSE PRIMER <400> 262 tgggttcaag tccttccatc gat 23 <210> 263 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11132 FORWARD PRIMER <400> 263 ctaactcatc aatgaaggcc tgctg 25 <210> 264 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS11132 REVERSE PRIMER <400> 264 ggacaagggc acagtctcca aaa 23 <210> 265 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS11133 FORWARD PRIMER <400> 265 gtgcttgctt ggcaatcacc att 23 <210> 266 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11133 REVERSE PRIMER <400> 266 ctccacagtt catctatcac ccgaa 25 <210> 267 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11134 FORWARD PRIMER <400> 267 gcttctcgat agtttgatgc attgc 25 <210> 268 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11134 REVERSE PRIMER <400> 268 gcaactcgca gatagaacct ggaat 25 <210> 269 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11135 FORWARD PRIMER <400> 269 aggtaacgga cagtcctgat tgacc 25 <210> 270 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS11135 REVERSE PRIMER <400> 270 gatgcatgag gccagtaaga ttgg 24 <210> 271 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS11136 FORWARD PRIMER <400> 271 tggccgagtc tttctctgcg tata 24 <210> 272 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11136 REVERSE PRIMER <400> 272 cgagatgatg aatgcaagga cttga 25 <210> 273 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11137 FORWARD PRIMER <400> 273 aaatcgtgaa gcagatcatg aaccg 25 <210> 274 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS11137 REVERSE PRIMER <400> 274 gcattgagtc gaaaacggtt ccttt 25 <210> 275 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12138 FORWARD PRIMER <400> 275 gactgacttt gccatcatga ccttg 25 <210> 276 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS12138 REVERSE PRIMER <400> 276 ccaacagcaa gagcaaggca gata 24 <210> 277 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12139 FORWARD PRIMER <400> 277 tcttcttttg gaacagtgat ggctg 25 <210> 278 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12139 REVERSE PRIMER <400> 278 gcaccggttg tgtctgaaag aagta 25 <210> 279 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12140 FORWARD PRIMER <400> 279 cctttgtgtg gaaaacgcaa gtaaa 25 <210> 280 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12140 REVERSE PRIMER <400> 280 gccatgtaaa aggcatcctg tcaaa 25 <210> 281 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12141 FORWARD PRIMER <400> 281 actccagctt aaaaagggat ccgag 25 <210> 282 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS12141 REVERSE PRIMER <400> 282 tattgtagtt ggcagggcca tgtc 24 <210> 283 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RS12142 FORWARD PRIMER <400> 283 gaggcaatcc atcaccatca ggt 23 <210> 284 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12142 REVERSE PRIMER <400> 284 gcggctctta aagtttgatg gacat 25 <210> 285 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12143 FORWARD PRIMER <400> 285 ttttgagttc ccaccaagag aatcc 25 <210> 286 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12143 REVERSE PRIMER <400> 286 gcgagaagat atacggaacg gtgtt 25 <210> 287 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS12144 FORWARD PRIMER <400> 287 taatggagct ttcacggttc atgc 24 <210> 288 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12144 REVERSE PRIMER <400> 288 tcgtggtatg taactatggc cgaca 25 <210> 289 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12145 FORWARD PRIMER <400> 289 agaatgataa tttgtgtggc gctga 25 <210> 290 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RS12145 REVERSE PRIMER <400> 290 tgcacggtcc ttctacgtta atgg 24 <210> 291 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12146 FORWARD PRIMER <400> 291 tgatctggca atcaagtcat gaagg 25 <210> 292 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RS12146 REVERSE PRIMER <400> 292 ggcgattcct gtgtactgtt ggttt 25

Claims (4)

delete 1 and 2 for RS011, a pair of primers represented by SEQ ID NOs: 3 and 4 for RS0110, a pair of primers represented by SEQ ID NOs: 5 and 6 for RS0111, a pair of SEQ ID NOs: 7 and 8 for RS0112 , Primer pairs shown in SEQ ID NOs: 9 and 10 for RS0113, primer pairs shown in SEQ ID NOs: 11 and 12 for RS0114, primer pairs shown in SEQ ID NOs: 13 and 14 for RS0115, primer pairs shown in RS0116 A pair of primers represented by SEQ ID NOs: 15 and 16 for RS0117, a pair of primers represented by SEQ ID NOs: 17 and 18 for RS0117, a pair of primers represented by SEQ ID NOs: 19 and 20 for RS0118, a pair of primers represented by 21 and 22 for RS0119 Pair, a pair of primers represented by SEQ ID NOs: 23 and 24 for RS012, a pair of primers represented by SEQ ID NOs: 25 and 26 for RS0120, a pair of SEQ ID NOs: 27 and 28 for RS0121 The primer pair shown in SEQ ID NOs: 29 and 30 for RS0122, the primer pair shown in SEQ ID NOs: 31 and 32 for RS0123, the primer pair shown in SEQ ID NOs: 33 and 34 for RS0124, the sequence for RS0125 37 and 38 for RS013, a pair of primers represented by SEQ ID NOs: 39 and 40 for RS014, a pair of primers represented by SEQ ID NOs: 41 and 42 for RS015, a pair of primers represented by SEQ ID NOs: Primer pair shown in SEQ ID NO: 43 and 44 for RS016, primer pair shown in SEQ ID NO: 45 and 46 for RS017, primer pair shown in SEQ ID NO: 47 and 48 for RS018, SEQ ID NO: 49 And 50, a pair of primers represented by SEQ ID NOS: 51 and 52 for RS0226, a pair of primers represented by SEQ ID NOS: 53 and 54 for RS0227, a pair of primers represented by RS0 57, and 58 for RS0229, a pair of primers represented by SEQ ID NOs: 59 and 60 for RS0230, a pair of primers represented by SEQ ID NOs: 61 and 62 for RS0231, a pair of primers represented by SEQ ID NOs: , Primer pairs shown in SEQ ID NOs: 63 and 64 for RS0232, primer pairs shown in SEQ ID NOs: 65 and 66 for RS0233, primer pairs shown in SEQ ID NOs: 67 and 68 for RS0234, primer pairs shown in RS0235 The primer pair shown in SEQ ID NOs: 69 and 70 for RS0236, the primer pair shown in SEQ ID NOs: 71 and 72 for RS0236, the primer pair shown in SEQ ID NOs: 73 and 74 for RS0237, and the SEQ ID NOs: 75 and 76 for RS0238 Primer pair shown in SEQ ID NO: 77 and 78 for RS0239, primer pair shown in SEQ ID NO: 79 and 80 for RS0240, sequence for RS0241 A primer pair represented by SEQ ID NO: 81 and 82, a primer pair represented by SEQ ID NO: 83 and 84 for RS0242, a primer pair represented by SEQ ID NO: 85 and 86 for RS0243, a primer pair represented by SEQ ID NO: 87 and 88 for RS0244 Pair, a pair of primers represented by SEQ ID NOs: 89 and 90 for RS0245, a pair of primers represented by SEQ ID NOs: 91 and 92 for RS0246, a pair of primers represented by SEQ ID NOs: 93 and 94 for RS0347, And 96, a pair of primers represented by SEQ ID NOs: 97 and 98 for RS0349, a pair of primers represented by SEQ ID NOs: 99 and 100 for RS0350, a pair of primers represented by SEQ ID NOs: 101 and 102 for RS0351, The primer pair shown in SEQ ID NO: 103 and 104 for RS0352, the primer pair shown in SEQ ID NO: 105 and 106 for RS0353, the SEQ ID NO: 107 and 1 08, a pair of primers represented by SEQ ID NOs: 109 and 110 for RS0355, a pair of primers represented by SEQ ID NOs: 111 and 112 for RS0356, a pair of primers represented by SEQ ID NOs: 113 and 114 for RS0457, a pair of primers represented by RS0458 117 and 118 for RS0459, a pair of primers represented by SEQ ID NOs: 119 and 120 for RS0460, a pair of primers represented by SEQ ID NO: 121 and 122 for RS0461 The primer pair shown in SEQ ID NO: 123 and 124 for RS0462, the primer pair shown in SEQ ID NO: 125 and 126 for RS0463, the primer pair shown in SEQ ID NO: 127 and 128 for RS0464, the primer pair shown in SEQ ID NO: The primer pair shown in SEQ ID NO: 129 and 130, the primer pair shown in SEQ ID NO: 131 and 132 for RS0466, the SEQ ID NO: 133 134, a pair of primers represented by SEQ ID NOs: 135 and 136 for RS0468, a pair of primers represented by SEQ ID NOs: 137 and 138 for RS0469, a pair of primers represented by SEQ ID NOs: 139 and 140 for RS0470, a pair of primers represented by RS0471 The primer pair shown in SEQ ID NO: 141 and 142, the primer pair shown in SEQ ID NO: 143 and 144 for RS0572, the primer pair shown in SEQ ID NO: 145 and 146 for RS0573, and the SEQ ID NO: 147 and 148 for RS0574 The primer pair shown in SEQ ID Nos. 149 and 150 for RS0575, the primer pair shown in SEQ ID Nos. 151 and 152 for RS0576, the primer pair shown in SEQ ID Nos. 153 and 154 for RS0577, the primer pair for RS0578 A pair of primers represented by SEQ ID NOs: 155 and 156, a pair of primers represented by SEQ ID NOs: 157 and 158 for RS0579, a pair of SEQ ID NO: 159 for RS0680 And 160, a pair of primers represented by SEQ ID NOs: 161 and 162 for RS0681, a pair of primers represented by SEQ ID NOs: 163 and 164 for RS0682, a pair of primers represented by SEQ ID NOs: 165 and 166 for RS0683, The primer pair shown in SEQ ID NOs: 167 and 168 for RS0684, the primer pair shown in SEQ ID NOs: 169 and 170 for RS0685, the primer pair shown in SEQ ID NOs: 171 and 172 for RS0686, the SEQ ID NOs: 173 and 174 , Primer pairs shown in SEQ ID NOs: 175 and 176 for RS0688, primer pairs shown in SEQ ID NOs: 177 and 178 for RS0689, primer pairs shown in SEQ ID NOs: 179 and 180 for RS0690, primer pairs shown in RS0691 181 and 182 for RS0692, a pair of primers represented by SEQ ID NOs: 183 and 184 for RS0692, a pair of primers represented by SEQ ID NO: 185 and 186, a pair of primers represented by SEQ ID NOs: 187 and 188 for RS07100, a pair of primers represented by SEQ ID NOs: 189 and 190 for RS07101, a pair of primers represented by SEQ ID NOs: 191 and 192 for RS07102 , Primer pairs shown in SEQ ID NOs: 193 and 194 for RS0794, primer pairs shown in SEQ ID NOs: 195 and 196 for RS0795, primer pairs shown in SEQ ID NOs: 197 and 198 for RS0796, SEQ ID NO: 199 for RS0797, 200, a pair of primers represented by SEQ ID NOs: 201 and 202 for RS0798, a pair of primers represented by SEQ ID NOs: 203 and 204 for RS0799, a pair of primers represented by SEQ ID NOs: 205 and 206 for RS08103, a pair of primers represented by RS08104 A pair of primers represented by SEQ ID NOs: 207 and 208 for RS08105, a pair of primers represented by SEQ ID NOs: 209 and 210 for RS08105, A pair of primers represented by SEQ ID NOs: 211 and 212, a pair of primers represented by SEQ ID NOs: 213 and 214 for RS08107, a pair of primers represented by SEQ ID NOs: 215 and 216 for RS08108, a pair of SEQ ID NOs: 217 and 218 for RS08109 A primer pair shown in SEQ ID NOs: 219 and 220 for RS08110, a primer pair shown in SEQ ID NOs: 221 and 222 for RS08111, a primer pair shown in SEQ ID NOs: 223 and 224 for RS08112, a primer pair shown in SEQ ID NO: 225 and 226, a pair of primers represented by SEQ ID NOs: 227 and 228 for RS09114, a pair of primers represented by SEQ ID NOs: 229 and 230 for RS09115, a pair of primers represented by SEQ ID NOs: 231 and 232 for RS09116 233 and 234 for RS09117, a pair of primers represented by SEQ ID NOs: 235 and 236 for RS10118, a pair of primers represented by RS 239 and 240 for RS10120, a pair of primers represented by SEQ ID NOS: 241 and 242 for RS10121, a pair of primers represented by SEQ ID NOS: 243 and 244 for RS10122 245 and 246 for RS10123, a pair of primers represented by SEQ ID Nos: 247 and 248 for RS10124, a pair of primers represented by SEQ ID N0: 249 and 250 for RS10125, a pair of primers represented by RS11126 A pair of primers represented by SEQ ID NOS: 251 and 252 for RS11127, a pair of primers represented by Nos. 253 and 254 for RS11127, a pair of primers represented by Nos. 255 and 256 for RS11128, and Nos. 257 and 258 for RS11129 The primer pair shown in SEQ ID NO: 259 and 260 for RS11130, the primer pair shown in SEQ ID NO: 261 and 262 for RS11131 A pair of primers represented by SEQ ID NOS: 263 and 264 for RS11132, a pair of primers represented by SEQ ID NOS: 265 and 266 for RS11133, a pair of primers represented by SEQ ID NOS: 267 and 268 for RS11134, 269 and 270, a pair of primers represented by SEQ ID NOS: 271 and 272 for RS11136, a pair of primers represented by SEQ ID NOS: 273 and 274 for RS11137, a pair of primers represented by SEQ ID NOS: 275 and 276 for RS12138 279 and 278 for RS12139, a pair of primers represented by SEQ ID NOS: 279 and 280 for RS12140, a pair of primers represented by SEQ ID NOS: 281 and 282 for RS12141, an Nos. 283 and 283 for RS12142, 284, a pair of primers represented by SEQ ID NOS: 285 and 286 for RS12143, a pair of primers represented by SEQ ID NOS: 287 and 288 for RS12144 And the primer pairs shown in SEQ ID NOS: 289 and 290 for RS12145 and the primer pairs shown in SEQ ID NOS: 291 and 292 for RS12146, A primer set for cleaved amplified polymorphic sequences (CAPS) marker amplification for genetic mapping to determine quantitative trait loci associated with stem diameters (QTLs).
3. The method according to claim 2, wherein the stem-related quantitative trait loci (QTL) of the recombinant subspecies of Milyang 23 and the rice paddy are located between RS0124 and RS0125 on chromosome 1 and the logarithm of the odds value is 3.26 , The allele of the rice paddy is located between QTL named "qI1D1", RM6841 and RS0578 on chromosome 5, which increases the thickness of the 1st section by 0.07 cm and is characterized by a 5.02% 1-fold phenotypic variation , An LOD value of 6.09, an allele of Miryang 23 increased by 0.10 cm in thickness of the first section, a QTL named "qI1D5" with a characteristic of showing a one-fold thickness phenotypic variation of 8.99%, between STS01039 and RS0124 on chromosome 1 , QTL named "qI3D1" with an LOD value of 3.17, an allele of the common rice with a 3-fold thickness variation of 0.15 cm and a 6.53% 3-fold phenotypic variation, or STS01039 on chromosome 1 And RS0124 , The LOD value is 4.61, and the allele of the reference rice is a QTL named "qI4D1", which increases the 4-fold thickness by 0.25 cm and exhibits a 9.97% 4-fold phenotypic variation. And a primer set for CAPS marker amplification for genetic mapping to determine the quantitative trait loci associated with the stem thickness of the recombinant control group of rice.
PCR was carried out using a primer set for CAPS marker amplification according to the second aspect of the recombinant strains of Milyang 23 and Korean rice paddy as a genetic mapping group and PCR was performed using the CAPS marker isolated by PCR , Milyang No. 23, and the recombinant control group of the representative rice paddy, in order to determine the stem-related quantitative trait locus.

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