KR101699232B1 - Micro device for selecting microalgae strains outstanding phototaxis or chemotaxis and selection method of microalgae strains using the same - Google Patents

Abstract

The present invention relates to a microdevice for microbial strain selection and a microbial strain selection method using the same.
The microdermabrasion microdevice for screening microbial strains according to the present invention and the method for screening microbial strains using the microdermabrasion microbes according to the present invention can rapidly select microbial strains sensitive to light and chemical stimuli. In particular, when a carbon source or a nitrogen source is supplied as the chemical stimulus, it is possible to specifically select microalgae strains having excellent growth characteristics.

Description

TECHNICAL FIELD The present invention relates to a micro-algae strain selecting micro-organism, and more particularly, to a micro-algae microorganism strain selecting method using microalgae strains,

The present invention relates to a microdevice for microbial strain selection and a microbial strain selection method using the same.

Microalgae generally contain a pigment such as chlorophyll, carotenoid, phycobillins, etc. It refers to a single cell organism that synthesizes organic matter and cell growth through photosynthesis. Microalgae have a faster growth rate than plants and can produce large amounts of light energy and neutral lipids capable of converting carbon dioxide and inorganic materials into biodiesel. As a result of the rapid increase in fossil fuel consumption, Which is one of the alternatives to solve the global warming problem.

So far, several hundred thousand species of microalgae have been reported to exist in freshwater and marine ecosystem. Research and development for various purposes have been attempted, but they are faced with difficulties such as strain improvement for productivity improvement due to limitation of genetic engineering. Chlamydomonas reinhardtii is the most studied microalgae so far. It is considered that it is a model organism of microalgae because it is easy to genetically manipulate such as transformation and development of related tools as well as its genome sequence as compared with other strains. Therefore, it has been actively used for the study of photosynthesis mechanism of microalgae, and it has also been used for lipid-related research and hydrogen production research for biodiesel production.

Microalgae, including Chlamydomonas reinhardtii, have a sensor inside the chloroplast that detects light called an eyespot. In order to find suitable conditions for photosynthesis, it is known that the reaction of light is moving by moving the flagellum toward the light or escaping to the defense against the strong light. Were mainly concentrated on the optimization of photosynthesis in large - scale culture, and the studies on photoreaction and photosynthesis through the analysis of individual cell units were insignificant. Also, it is referred to as chemotaxis that exhibits chemical reactivity by a carbon source or a nitrogen source. In addition, research has been conducted on prokaryotic cells such as E. coli, but it is insignificant in the case of photosynthetic single-celled organisms such as microalgae. In order to utilize microalgae as an efficient energy source, many problems remain. One of the most important problems is that the light absorption rate of the chlorophyll antenna of the culture surface cell exposed to the high intensity light source in the mass culture system exceeds the photosynthetic efficiency, Heat and fluorescence, and the light energy transfer to the inner cells is not performed properly, resulting in a decrease in the overall light conversion efficiency. To overcome these limitations, studies have been conducted to develop a mutant strain that reduces the size of a chlorophyll antenna of a cell. It has been reported that a mutant strain having a reduced size of the chlorophyll antenna exhibits more efficient light conversion efficiency in mass culture. Therefore, it is essential to understand the correlation and mechanism of photoreactivity and photosynthesis by photoluminescence at the individual cell level in order to improve photosynthesis and light conversion efficiency in a microalga mass culture system.

In recent years, development of a microalga culture and analysis system using a micro system has been attempted. When a micro system is used, it is possible to miniaturize an analysis system, thereby reducing sample and expensive biochemical reagents, It is possible to reduce the analysis time and expense. The flow of uniform laminar flow without turbulence also simplifies the experimental conditions and minimizes external influences. In addition, reproducible analytical data can be obtained. In addition, cell-based data have been widely used for drug screening, pharmacokinetics, and toxicity analysis, but they are now being used for metabolism exchange or intracellular mechanism between cells and tissues.

PDMS (Polydimethylsiloxane), a macromolecular material used in micro systems, has no toxicity to living organisms and has the advantage of facilitating the transfer of substances necessary for biological activity by being porous. The PDMS-based microsystem can be custom designed for the environment that is suitable for the target and purpose to be analyzed. In particular, based on the high transparency and microstructure, it enables continuous and detailed monitoring of individual cells through an optical microscope and efficient high-speed line separation, thus contributing to deep understanding of the photoreaction and the response to compounds depending on the photostability and chemotaxis . However, efforts to observe the photosynthetic efficiency of microalgae strains using a microsystem are in short supply.

The prior art document related to the present invention discloses Korean Patent No. 10-1424855 (Patent Document 1), and Patent Document 1 discloses a fluorescence activated cell sorter (FACS) and Nile red, A method for rapidly isolating a microalgae strain having a high lipid content by using a hair dye and a method for regenerating the microalgae strain are disclosed.

Patent Document 1. Korean Patent No. 10-1424855

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a microdevice capable of rapidly screening microalgae strains excellent in reactivity against light and chemical stimuli or a method for selecting microalgae strains using the same The purpose is to provide. That is, it is an object of the present invention to provide a micro device capable of simultaneously measuring the photostability and the chemotaxis of a microalgae strain, and a method of selecting microalgae strains using the same.

According to an aspect of the present invention, there is provided a micro-

A microalgae strain inflow section, a nutrient source inflow channel, a nutrient source injection channel, a microalgae strain reaction section, a metering section, and a microalgae strain reaching section;

The microalgae strain inflow section, the microalgae strain reaction section, the metering section and the microalgae strain arrival section are sequentially connected and aligned on the plate; And

Wherein the nutrient source injection channel is formed by attaching the nutrient source injection channel to one side of the microalgae strain inlet, the microalgae strain reaction unit, the metering unit and the microalgae strait arrival unit sequentially aligned.

.

The method for selecting microalgae strains according to another aspect of the present invention uses the microarray,

1) introducing a microalgae strain into a microalgae strain inlet and injecting a nutrient source into a nutrient source inlet to cause microalgae strains to migrate toward the microalgae strains; And

2) measuring the microalgae strains reached at the metering section at the metering section, then selecting at the microalgal strain reaching section;

.

The microdermabrasion microdevice for screening microbial strains according to the present invention and the method for screening microbial strains using the microdermabrasion microbes according to the present invention can rapidly select microbial strains sensitive to light and chemical stimuli. In particular, when a carbon source or a nitrogen source is supplied as the chemical stimulus, it is possible to specifically select microalgae strains having excellent growth characteristics.

FIG. 1 is a schematic diagram illustrating a whole process of selecting strains having photosynthetic efficiency and carbon dioxide absorption ability by using photosynthetic microalgae strains in terms of daylighting and chemotaxis in a microdevice.
Figure 2 is a photograph of the microdevice used in the present invention.
Figure 3 is a device diagram showing the structure of a microdevice used in the present invention (the numerical values given are figures according to a preferred embodiment of each part).
FIG. 4 is a graph showing the fluorescence emission photographs of the outflow portion of the microdevice used in the present invention and the fluorescence intensity of the 0.1 mM fluorescent material over time in the device.
FIG. 5 is a graph showing the number of cells reaching eight outflows for 30 minutes depending on the bicarbonate concentration through the microdevice used in the present invention. FIG.
FIG. 6 is a graph showing the number of cells reached by eight outflows for 30 minutes for only wild-type strain, mutant strain 1, and mutant strain 2 when subjected to light stimulation only.
Figure 7 is a graph showing the number of cells reaching eight outflows for 30 minutes of wild type strain, mutant strain 1 and mutant strain 2 for 100 mM bicarbonate concentration.
FIG. 8 is a graph showing the growth performance of wild type strain, mutant strain 1 and mutant strain 2 used in the present invention.
9 is a graph showing the photosynthetic efficiency of the wild type strain, mutant strain 1, and mutant strain 2 used in the invention.
10 is a graph showing the skewness of the wild type strain, the mutant strain 1 and the mutant strain 2 graph obtained in FIG.
FIG. 11 is a graph showing the correlation between the growth performance of the wild type strain, the mutant strain 1 and the mutant strain 2 of FIG. 8, and the distortion value of the graph of each strain of FIG.
12 is a graph showing the correlation between the photosynthetic efficiency of the wild type strain, the mutant strain 1 and the mutant strain 2 of FIG. 9, and the distortion value of the graph of each strain of FIG. 10.

Accordingly, the inventors of the present invention have made extensive efforts to select microalgae strains having excellent daylighting and chemotaxis, and as a result, they have found a microarray for selecting microalgae strains according to the present invention and a method for selecting microalgae strains thereof, .

Specifically, the microdevice for microbial strain selection according to the present invention comprises

A microalgae strain inflow section, a nutrient source inflow channel, a nutrient source injection channel, a microalgae strain reaction section, a metering section, and a microalgae strain reaching section;

The microalgae strain inflow section, the microalgae strain reaction section, the metering section and the microalgae strain arrival section are sequentially connected and aligned on the plate; And

Wherein the nutrient source injection channel is formed by attaching the nutrient source injection channel to one side of the microalgae strain inlet, the microalgae strain reaction unit, the metering unit and the microalgae strait arrival unit sequentially aligned.

.

The micro-algae microinfection apparatus according to the present invention is characterized in that a nutrient source such as a carbon source or a nitrogen source is injected into the nutrient source injection channel and supplied to the microalgae strain through the nutrient source injection channel, It is possible to judge the growth potential, that is, the chemotaxis. In addition, in the case of measuring the chemotaxis through the micro device under an environment in which light is irradiated, it is possible to measure not only the chemotaxis but also the photometric property. In addition, even if nutrient source is fed to the feeding channel of the nutrient source and the nutrient source is fed to the microalgae reaction unit through the feeding channel, the supplied nutrient source concentration becomes lower as the distance from the feeding channel increases. The drawing phenomenon can be performed more precisely. Therefore, it is possible to measure the number of cells reached for a certain period of time according to the chemotaxis and chemotaxis of the microalgae strains according to the concentration gradient. Also, the microalgae strains excellent in reactivity to the mainstream and chemotaxis can be selected from the microalgae strains reaching section, and can be selected from the zones classified according to the concentration of the nutrient source. To this end, (Storage tank).

Meanwhile, the microalgae strains inlet, the microalgae strainer reactors, the measuring unit and the microalgae strainer reaching unit may be sequentially connected and aligned on a plate. At this time, the microalgae strain inlet and the microalgae strain reaction section can be connected by the connection channel, whereby the microalgae strains can be moved to the microalgae strain reaction section. Preferably, the microalgae strain inflow portion may have a diameter of 4 to 10 mm. Also, the width of the connection channel may preferably be 0.1-16 mm. The microalgae cultured in the microalgae strain reaction part can reach to the microalgae strains after reaching the metering part and measuring the growth ability according to their daylighting or chemotaxis. At this time, the microalgae strains can be sorted by each zone (storage tank) according to the order of arrival according to the concentration gradient of the nutrient source and reactivity to daylight.

The nutrient source injected through the nutrient source injection channel may be passed through the nutrient source injection channel and then transferred to the microalgae strain reaction unit to supply the nutrient source to the microalgae strains. The nutrient source is not particularly limited as long as it can be used as a nutrient source of microalgae strains, but it may preferably be a carbon source or a nitrogen source. Meanwhile, the width of the nutrient source injection channel may be preferably 1-4 mm, and the width of the nutrient source injection channel may preferably be 0.010-0.020 mm.

In addition, the nutrient source injection channel may pass the nutrient source to the microalgae reaction unit through a plurality of nutrient inlet ports 160. When the nutrient source is divided and supplied through the plurality of the nutrient inlet ports, It is possible to supply the nutrient source at a uniform concentration over the whole area attached to the plant. On the other hand, the width of the nutrient inlet may be preferably 0.01-0.05 mm.

In addition, although there is no particular limitation, the microalgae strain reaction unit may preferably have a length of 1-10 mm and a width of 0.1-1 mm.

In addition, the microalgae strain reaction unit may be a microalgae strain that uses a nutrient source or light supplied through the nutrient source injection channel. That is, since the microdevice according to the present invention is to select a microalgae strain that grows in response to a nutrient source or light, it may be a microalgae strain having excellent photostability or chemotaxis. Also, in the present invention, the main light property refers to a property of reacting with light, and the chemotactic property may be a term that includes a property of reacting to a chemical stimulus and sensitive to a chemical nutritional source. In addition, the microalgae strain reacting unit may move the microalgae strain to a preferred chemical concentration while using the nutrient source or light supplied through the nutrient source injection channel.

Also, it is preferable that the measuring unit has a width of 0.05-0.2 mm, and it is possible to measure the growth of the microalgae strains more precisely and precisely as long as the width of the measuring unit falls within the above-mentioned numerical range. In addition, the length of the measuring portion may be preferably 1-4 mm.

In addition, the microalgae strains reaching portion preferably comprises one or a plurality of reservoirs having a diameter of 1-2 mm. The microalgae strains which have passed through the measuring unit can be stored in the storage tank and sorted. In addition, the microorganism strains can be selected in the storage tank by dividing the microorganism strains into a plurality of storage tanks according to the growth order.

In addition, the present invention provides a mutant strain of a photosynthetic single-celled organism, which is more easily reacted with a photoreactive substance than a conventional method for selecting a strain by biochemical analysis, The number of cells can be selected by statistical analysis such as peak histogram analysis, graph distortion analysis, and number of cells reached.

Particularly, it is possible to analyze the strains reacting with light on an optical microscope from a microscopic viewpoint, an individual cell unit, and also to perform a statistical analysis on a strain cluster easily through a micro apparatus having excellent monitoring, But is not limited to monitoring on an optical microscope.

In addition, the present invention is based on the finding that various indicators (histogram peak, number of reaching cells, analysis of distortion of the graph, etc.) obtained by statistical analysis of the photoreactivity of individual photosynthetic single-celled organisms and chemical reactivity to carbon sources, And photosensitivity to compounds, as well as the effects of chlorophyll-related mechanisms and factors on photosynthetic activity, such as chlorophyll, caused by mutation, It is possible to more easily search for the change and to contribute to the effective selection of strains having excellent photosynthesis and light conversion efficiency.

In addition, by using the measurement method including the analysis of the growth of the mutant strain, the correlation between the photostability and the chemotaxis in the selection of the strain having the excellent ability to absorb carbon dioxide of the mutant strain was proved. It is possible to contribute to

In accordance with another aspect of the present invention, there is provided a method of selecting microalgae strains using the microarray.

Specifically, the micro device is used,

1) introducing a microalgae strain into a microalgae strain inlet and injecting a nutrient source into a nutrient source inlet to cause microalgae strains to migrate toward the microalgae strains; And

2) measuring the microalgae strains reached at the metering section at the metering section, then selecting at the microalgal strain reaching section;

.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Example

< Example  1: Various Light sensitive  &Lt; / RTI &gt; and preparation of mutant strains with reaction characteristics to compounds &

The strain used in this example was Chlamydomonas renihardtii, and the wild type strain used for the production of the mutant strain was CC125. From this wild type strain, electroporation (Chlamydomonas reinhardtii) ), And mutagenic strains were obtained. Various mutant strains were analyzed for photoreactivity due to photostability in a microdevice and reaction characteristics for compounds according to carbon source concentration by chemotaxis.

The medium used in this experiment is TAP-C medium, and the constituents thereof are shown in Table 1 below.

< Example  2: Cultivation of microalgae and production of selective microdevices based on daylighting / chemotaxis>

The Chlamydomonas reinhardtii wild-type strain (CC125) and the mutant strain were cultured in TAP agar medium in a TAP-C liquid medium for 24 hours at 40 μmol photon m? Lt; 0 &gt; C for 2 days. This is an adaptation process for increasing the chemical reactivity to the carbon source and for controlling it efficiently and constantly. The cells were cultured for 2 days and then diluted to a certain cell concentration (7.5 × 10 ⁴ cells ml- ¹ ) at the exponential phage stage and stored in a dark room for 30 minutes. This is an adaptive process for efficiently and constantly controlling the photoreactivity and photosensitivity of cells to light.

The microdevice used in the present invention was manufactured by spin-coating a negative photoresist SU-850 on a silicon substrate, covering the designed mask and exposing it to ultraviolet rays using an ultraviolet ray exposer to perform photo-lithography, Polydimethylsiloxane (PDMS) and a curing agent were mixed at a ratio of 10: 1 and formed on a SU-8 mold manufactured by photolithography. The completed PDMS microdevice was coupled with a slide glass through plasma treatment. Subsequently, 2% liquid agarose at 60 ° C is injected into the microchannel portion and solidified to form a stable and constant carbon source concentration gradient in the microdevice. For carbon source concentration gradients in the microdevice, add 100 mM bicarbonate and allow a constant concentration gradient to form for 6 hours.

Then, 40 [mu] l of the cells stored in the dark room for 30 minutes were added to the Inlet portion of Fig. 1, and 40 [mu] l of TAP-C medium was placed in the Out let portion. And the number of cells reached at 8 outlets on an optical microscope after 30 minutes was observed.

< Example  3 In the micro device Fluorescence Factor  Constant and consistent use Concentration gradient  Check>

As shown in FIG. 4, in order to confirm whether the concentration gradient of the carbon source in the micro device is maintained constantly, a fluorescent material having a molecular weight similar to that of bicarbonate is used, And the fluorescence intensity was measured. As a result, the fluorescence intensities were increased due to the fact that the fluorescent material did not diffuse to all the eight reaching portions until the fluorescent material was added for 6 hours. After 6 hours, the fluorescent material reaches the eighth reaching part, and the fluorescence intensity of each reaching part is constantly maintained. These results demonstrate that it is possible to analyze the reaction characteristics of the compounds according to the concentration gradient of the carbon source as well as the light reaction by the light because the concentration gradient of the carbon source in the micro device is maintained constant after 12 hours.

< Example  4: In micro device Carbon source  Depending on concentration Photoreaction  And reaction characteristics of compounds>

As shown in FIG. 5, in order to determine carbon source concentration gradient conditions for efficient selection in a microdevice, initial concentrations of bicarbonate were varied in wild type strains. The initial concentrations of bicarbonate were 0, 30, 50, 100, and 200 mM, and 0 mM indicates cell migration by light stimulation alone. As a result, when the light stimulus was given, almost the same number of cells reached 8 reaching sites, and as the concentration increased to 30, 50, and 100 mM, the concentration of the carbon source in the micro- We confirmed the results that reached a lot. In addition, it was confirmed that most of the cells were present at the 6, 7, and 8 times of the low concentration when the carbon source having a high concentration of 200 mM was used. On the basis of this, in order to compare the response characteristics of the wild type strain and the mutant strain to the photoreaction and the compound, the concentration of 100 mM of carbon source close to the histogram of the ideal normal distribution is used as a reference.

< Example  5: Strain by light stimulus only Photoreaction  And Light sensitive  Analysis>

As shown in FIG. 6, when the light stimulus was applied, it was found that the number of cells was almost equal to that of the eight reaching regions by moving for a predetermined time (30 minutes). Not only the wild type strain as a control but also the mutant strain 1 and the mutant strain 2 show the same result. When only light stimulation was given, the differences in each strain were confirmed to be the total number of cells reached. The mutant strain 1 with excellent photoreactivity and photosensitivity showed a larger number of cells than that of the control group by moving for a certain period of time, and the mutant strain 2 with low photoreactivity and photosensitivity showed a smaller number of cells than the control group Respectively.

< Example  6: By light and chemical stimulation Photoreaction  And reaction characteristics of compounds>

In order to analyze the reaction characteristics of the compound by light source and simultaneous carbon source concentration gradient, a concentration gradient of carbon source was formed with bicarbonate at an initial concentration of 100 mM. Then, the wild type strain, the mutant strain 1, and the mutant strain 2 of the control group were placed in a microdevice and the light source was irradiated to the entrance portion for a predetermined time (30 minutes), and the number of cells reaching 8 reaching after 30 minutes Respectively. As a result, the mutant strain 1 was found to have a large number of cells reaching the second and third reaching parts having a higher carbon source concentration than the wild type strain as a control group. In addition, the number of total cells reached for a certain period of time was confirmed to be larger than that of the control group. Based on this, it was confirmed that the histogram represented by the number of cells reached by the reaching region was biased to the left as compared with the control group. On the other hand, in the case of the mutant strain 2, the number of the cells reaching the 6, 7, 8 reaching the lower carbon source concentration was larger than that of the wild type strain, which was the control group, and the number of total cells reached in a certain time was smaller. Based on this, it was confirmed that the histogram indicated by the number of cells reached was biased to the right as compared with the control group.

< Example  7: Analysis of growth, photosynthetic efficiency, and skewness of wild type and mutant strains>

The wild type and mutant strains 1 and 2, which were used to select strains with excellent photosynthetic efficiency and growth potential, were evaluated for their growth potential, photosynthetic efficiency, The results of the analysis were also analyzed.

First, as shown in FIG. 8, growth characteristics of each strain were analyzed. For growth analysis, initial inoculation concentrations were inoculated into TAP-C medium at a concentration of 0.1 at 800 nm wavelength using the absorbance method, and cultured for 6 days. Using the difference between the maximum and minimum concentrations, Growth potential. As a result, based on the wild type strain as the control group, the mutant strain 1 showed about 2 times or more growth potential and the mutant strain 2 showed 0.5 times the growth ability.

Next, the efficiency of intracellular photosystem was measured to show photosynthetic efficiency. Higher photosystem efficiency means higher photosynthetic efficiency, and lower photosystem efficiency means less photosynthetic efficiency. As shown in FIG. 9, mutant strain 1 had higher photosynthetic efficiency and mutant strain 2 had lower photosynthetic efficiency than the control group.

In order to analyze the correlation between growth and photosynthetic efficiency of the strain obtained in FIG. 8 and FIG. 9, the degree of distortion of the graph was analyzed in the histogram obtained in FIG. The strains with excellent photoreactivity and photosensitivity to light and reaction characteristics to compounds due to carbon source concentration gradients have a positive histogram distortion value and a low degree of diurnal and chemotaxis. The histogram is shifted to the right, and the degree of distortion of the graph has a negative value. In other words, the control group is close to the normal distribution and the distortion degree is close to zero. In the case of the histogram of the mutant strain 1, the histogram is biased to the left and has a positive value distortion. On the other hand, in the case of the mutant strain 2, the histogram is biased to the right and has negative distortion.

< Example  8: Analysis of correlation between growth and photosynthesis efficiency and daylight /

Based on the microdevice invented in this study, correlation between growth and photosynthetic efficiency of the indicator and the growth and photosynthetic efficiency of the microorganism were investigated.

First, as shown in FIG. 11, the correlation between the value shown in FIG. 10 and the growth performance of the strain shown in FIG. As a result of the analysis, the correlation index R ² = 0.95 shows a high correlation, indicating that the strain having excellent reactivity to daylight and chemotaxis has a high growth potential of the strain.

Next, as shown in FIG. 12, the correlation between the disturbance of the photostability index and the photosynthetic efficiency of the strain shown in FIG. 9 was analyzed. As a result of the analysis, correlation coefficient R ² = 0.985 showed a high correlation. This indicates that the photosynthetic efficiency of the strain is higher as the strain having excellent reactivity to daylight and chemotaxis.

In other words, in FIGS. 11 and 12, it was confirmed that strains having excellent growth and photosynthetic efficiency of strains can be easily selected by the microdevice and selection method of the present invention.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. It is natural.

100. Microalgae strain inflow section
110. Nutrient Infusion Oil
120. Nutrient injection channel
130. Microalgae strain reaction unit
140. Measurement Section
150. Microalgae strains reaching portion
160. Nutrient inlet

Claims (10)

A microalgae strain inflow section, a nutrient source inflow channel, a nutrient source injection channel, a microalgae strain reaction section, a metering section, and a microalgae strain reaching section;
The microalgae strain inflow section, the microalgae strain reaction section, the metering section and the microalgae strain arrival section are sequentially connected and aligned on the plate; And
Wherein the nutrient source injection channel is formed by attaching the nutrient source injection channel to one side of the microalgae strain inlet, the microalgae strain reaction unit, the metering unit and the microalgae strait arrival unit sequentially aligned.
A micro-algae strain selecting micro-apparatus.
The method according to claim 1,
Wherein the nutrient source injected through the nutrient source injection channel passes through the nutrient source injection channel and then moves to the microalgae strain reaction unit to supply a nutrient source to the microalgae strain.
3. The method of claim 2,
Wherein the nutrient source injection channel passes a nutrient source through a plurality of nutrient inlet ports to a microalgae strain reaction unit.
The method according to claim 1,
Wherein the microalgae strains have a diameter of 4 to 10 mm and feed microalgae strains to the microalgae strains through a connection channel connected to the microalgae strains reacting unit.
The method according to claim 1,
Wherein the microalgae strain reaction unit has a length of 1-10 mm and a width of 0.1-1 mm.
The method according to claim 1,
Wherein the microalgae strains react to the microalgae strains using a nutrient source or light supplied through the nutrient source injection channel to the microalgae strains.
The method according to claim 1,
Wherein the measuring unit has a width of 0.05-0.2 mm.
The method according to claim 1,
Wherein the microalgae strains reaching portion comprises one or a plurality of reservoirs having a diameter of 1-2 mm.
A method for selecting microalgae strains using a microarray for sorting microalgae strains according to claim 1.
10. The method of claim 9,
The method for selecting microalgae strains uses the microarray according to claim 1,
1) introducing a microalgae strain into a microalgae strain inlet and injecting a nutrient source into a nutrient source inlet to cause microalgae strains to migrate toward the microalgae strains; And
2) measuring the microalgae strains reached at the metering section at the metering section, then selecting at the microalgal strain reaching section;
Wherein the microbial strain is selected from the group consisting of:

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