KR101693103B1 - Electrode generation method for dielectrophoresis based particle separation or capture - Google Patents

Electrode generation method for dielectrophoresis based particle separation or capture Download PDF

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KR101693103B1
KR101693103B1 KR1020150049961A KR20150049961A KR101693103B1 KR 101693103 B1 KR101693103 B1 KR 101693103B1 KR 1020150049961 A KR1020150049961 A KR 1020150049961A KR 20150049961 A KR20150049961 A KR 20150049961A KR 101693103 B1 KR101693103 B1 KR 101693103B1
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electrode
electric field
electrodes
calculation step
factor
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KR1020150049961A
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Korean (ko)
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KR20160120870A (en
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장재성
한창호
하현욱
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울산과학기술원
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells

Abstract

It is an object of the present invention to provide a method of generating electrodes for collecting and collecting particles based on dielectrophoresis which produces new types of microelectrodes capable of achieving optimal separation and collection efficiency in the same channel structure.
According to an aspect of the present invention, there is provided a method of forming electrodes for collecting and collecting particles based on dielectrophoresis, the method comprising: setting a straight electrode; An electrode discretization step of disposing the plurality of linear electrodes horizontally in the channel length direction; An electric field strength calculation step of calculating electric field intensities for each type of power source by setting various types of power sources to the discrete electrodes; And a final electrode calculation step of selecting an electrode shape of the maximum electric field intensity among the set power sources.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing an electrode for use in dielectrophoresis-based particle separation and collection apparatus,

The present invention relates to a method for producing an electrode for a particle separation and collection device based on dielectrophoresis, and more particularly, to a method for producing an electrode for collecting and separating particles based on dielectrophoresis to produce an electrode capable of producing excellent separation and collection efficiency will be.

Dielectrophoresis is a phenomenon in which dielectric particles that are not free-charged move to a larger or smaller electric field under a non-uniform electric field. In general, the magnitude of the force that can be obtained by such a dielectrophoresis phenomenon is very small, so that there is no example used in the conventional general mechanical system. However, recently, as the field of micro electro mechanical system (MEMS) As its size gets smaller, its use is rapidly increasing.

The above-mentioned dielectrophoresis phenomenon is widely used in the field of bio-MEMS, for example, separation of dead cells and living cells, separation of cancer cells and normal cells, separation of particles of different sizes or types, Etc., trapping, and changing the transport path.

The dielectrophoresis phenomenon of the above characteristic can be operated by only the electrode without any moving means and there is no need to label the sample. Especially, in the case of MEMS, since it can operate even at a small voltage of 10 V or less, And the success rate is very high in the case of cell separation and the like, and various configurations of the methods are proposed.

For example, Japanese Patent Application Laid-Open No. 2008-0087404 discloses a plasma display panel comprising a lower substrate, a plurality of strip-shaped first electrodes stacked in parallel on the upper surface of the lower substrate, And a second electrode formed on the spacer and covering the lower substrate and a second electrode coated on at least a part of the lower surface of the upper substrate, .

Also, Japanese Patent Application Laid-Open No. 2009-0002980 discloses a micro fluid flow path including minute particles; An electrode that forms an electric field to move the microparticles by dielectrophoresis with a voltage applied from a voltage source; And an insulator structure configured to deform the electric field to concentrate the fine particles by positive dielectrophoresis; And a microparticle treatment apparatus comprising the microparticle treatment apparatus.

As described above, a device for separating particles based on dielectrophoresis generally has a structure in which alternating voltage is applied to interdigitated electrodes (IDE) at the bottom of channels to separate or collect the particles passing through the channels according to the electrical properties of the particles , It is widely used for the separation and collection of fine particles.

However, in the separation apparatus according to the above method, when the flow velocity is small, the separation performance of the particles is excellent, but when the flow velocity is high, the inertial force due to the flow is larger than the electric force on the particles, Patent Application No. 2014-0029285.

The above patent discloses that a partition wall is provided on a sidewall of a channel formed in the inner side to exhibit a collecting efficiency similar to that of a conventional one at a low flow rate and to reduce the inertial force of particles by the partition wall when the fluid flow rate increases, It is possible to prevent collection and reduction of particle separation efficiency.

However, the improvement of the separability according to the shape of the electrode is not considered in the conventional method in which a plurality of electrodes are provided perpendicularly in the longitudinal direction of the channel.

The present invention has been made in order to overcome the disadvantages of the prior art as described above, and it is an object of the present invention to provide a method and apparatus for separating and collecting dielectrophoretic particles, which can produce a new type of microelectrode capable of achieving optimal separation and collection efficiency in the same channel structure It is an object of the present invention to provide a method of generating an electrode.

According to an aspect of the present invention, there is provided a method of generating electrodes for collecting and collecting particles based on dielectrophoresis, the method comprising: a plurality of linear electrodes disposed vertically in a channel length direction; An electrode discretization step of disposing the plurality of linear electrodes horizontally in the channel length direction; An electric field strength calculation step of calculating electric field intensities for each type of power source by setting various types of power sources to the discrete electrodes; And a final electrode calculation step of selecting an electrode shape of the maximum electric field intensity among the set power sources.

Preferably, the electric field strength calculation step includes a power setting step, an electrode connection step of connecting the electrodes to the set power state, and a factor calculation step of calculating the electric field strength based on the connected dioxide electrode .

More preferably, in the factor calculation step, the electric field intensity in the x direction (channel length direction) of the planar angle grid i with the connected dioxide electrode is E xi and the electric field intensity in the y direction (vertical direction in the x direction) E yi , the factor of the grid i is E xi 2 + E yi 2 , and the overall factor S is the sum of the factors in all the grids for the connected dioxide electrode.

More preferably, in the final electrode calculation step, the electrode type having the highest total factor (S) value is selected.

More preferably, in the electrode discretization step, the number of electrodes discretized from the single elongated electrode is three or more.

The method for generating electrodes for dielectrophoretic-based particle separation and collecting apparatus according to the present invention is a method of disposing a commonly used electrode (IDE), calculating the intensity of an electric field at a discretized electrode, The present invention proposes a new type of microelectrode that is connected in a planar manner. The proposed microelectrode provides higher collection efficiency than a conventional straight electrode.

FIG. 1 is a flow chart of a method for separating and collecting dielectrophoretic based particles according to the present invention,
2 is an example of a prepared straight electrode,
Figure 3 is an example of discretizing Figure 2,
4 is voltage modeling at the discretized electrode,
5 is voltage modeling when the intermediate voltage value is known,
Figure 6 is voltage modeling near Figure 5,
Figure 7 is voltage modeling under different conditions,
8 is a boundary condition along one side,
9 is a boundary condition between two sides,
10 is a boundary condition between one region and two sides,
11 is an electric field intensity of a straight electrode,
12 is the electric field intensity of the reference electrode,
13 shows the electric field strength of the electrode according to the optimum value,
14 is a graph showing the dust collecting efficiency according to three electrodes,
Fig. 15 is a table showing the change of the factor value according to the number of grids per electrode.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

As shown in FIG. 1, a method of forming electrodes for collecting and collecting dielectrophoretic-based particles according to the present invention includes a linear electrode setting step S1, an electrode discretization step S2, an electric field strength calculation step S3, And a calculating step S4.

Each step will be described in detail below.

The straight electrode setting step (S1)

The straight-type electrode setting step S1 is a step of setting the electrodes formed in a straight line with respect to the conventional channel width.

At this time, as shown in FIG. 2, the conventional electrodes are uniformly arranged with a large number (six) of the conventional electrodes 2 arranged vertically in the longitudinal direction of the channel 1.

At this time, the conventional electrodes 2 are arranged at equal intervals with the same width, and the polarity of the connected power source is repeatedly arranged. That is, they are arranged as +, -, +, or may be arranged as -, +, -. In Fig. 2, the same poles are made of the same color.

On the other hand, if necessary, the conventional electrode 2 may be arranged at a different interval from the other widths.

Electrode discretization step (S2)

The electrode discretization step S2 is a step of discretizing the conventional electrode 2 set through the straight type electrode setting step S1 into a plurality of electrodes.

At this time, the continuous conventional electrode 2 is divided into a plurality of divided electrode groups 3.

FIG. 3 shows an example in which six conventional electrodes 2 shown in FIG. 2 are composed of seven discrete electrodes 3, but if necessary, the number of discrete electrodes 3 can be different and advantageous as the number increases. However, The calculation time is increased.

It is preferable that the number of the above-mentioned electrodes 3 is three or more per conventional electrode 2.

If the number of the electrodes 3 is less than 3, it means 1 or 2, and if it is 1, it is the same as that of the conventional electrode 2, which is inadequate.

Electric field calculation step (S3)

The electric field calculation step S3 is a step of calculating the electric field intensity of the electrode 3 produced through the electrode discretization step S2.

The electric field calculation step S3 comprises three sub-steps.

That is, a power supply setting step of setting the power supply state to the entire electrode 2, an electrode connection step of connecting the electrodes 2 to the set power supply state, and a step of calculating the electric field strength based on the connected electrode 2 Factor calculation step.

The power setting step allocates each power state to 50% in a step of assigning the power state of each of the electrodes 3 to + or -.

At this time, it is set except for the state where the power is not applied because the electrode is not connected to the side surface of the electrode (3).

Then, an electrode connection step for connecting the respective electrodes 2 is performed.

The factor calculating step calculates a factor indicating the electric field intensity based on the electrode state set in the power setting step.

The above sub-steps are not repeatedly performed once, and when the electrode state is changed in the power source setting step, the electrode is connected in the changed state, and the factor indicating the electric field strength is repeatedly performed .

Hereinafter, the factor S representing the dielectrophoretic force will be described.

First, when the dislocation electrode 3 is discretized into a two-dimensional grid (hxh), the voltage can be expressed as shown in Fig.

And, when applying the Laplace equation and the boundary conditions, the magnitude (S) of the dielectrophoretic force is calculated as follows.

Laplace equation:

Figure 112015034530960-pat00001

Applying the Finite Difference Method (FDM) to each term of the above Laplace equation,

Figure 112015034530960-pat00002

The above equation is substituted into the Laplace equation and expressed as follows.

Equation 1:

Figure 112015034530960-pat00003

If the voltage grid is located in the middle of the electric field, the relationship shown in FIG. 5 can be obtained.

Equation 2:

V i = V 0

When the condition of FIG. 6 is applied, the following equation can be obtained.

Equation (3)

Figure 112015034530960-pat00004

If the voltage is known at the two positions shown in FIG. 7, the following equation can be obtained.

Equation 4:

Figure 112015034530960-pat00005

Then, as shown in FIG. 8, the following equation can be obtained under the condition that the upper end is insulated (Neumann).

Equation 5:

Figure 112015034530960-pat00006

In the case where the two sides shown in Fig. 9 are insulated, the following equation can be obtained.

Equation (6)

Figure 112015034530960-pat00007

As shown in FIG. 10, the condition for the case where the lower side is insulated and the voltage value on the other side is specified is given by the following equation.

Equation (7)

Figure 112015034530960-pat00008

Equation (2) to Equation (7) correspond to a boundary condition, so that the following electric field strength can be calculated when applied to each condition.

Electric field strength:

Figure 112015034530960-pat00009

Unit factor:

Figure 112015034530960-pat00010

And the electric field strength or dielectric constant factor S:

Figure 112015034530960-pat00011

Where V is the electric potential, E is the electric field strength, x and y are the two-dimensional coordinates, and i is the respective grid number on the plane with the connected dioxide electrode. Thus S is the sum of the factors in all the grids (including on the electrode and the inter-electrode channel plane) for the connected dioxide electrode.

Therefore, the S value is calculated at the set electrode 3 every time.

Final electrode calculation step S4

The final electrode calculation step S 4 is a step of calculating the S value by changing the shape of the electrode in the electric field strength calculation step S 3 and calculating the final electrode by selecting the electrode arrangement having the highest S value.

Example

A new type of electrode was calculated using six conventional electrodes as shown in FIG. 11 as a starting electrode.

At this time, the electric field analysis result of the conventional electrode is also shown on the right side of FIG. 11, and the calculated S value is 87.400.

Then, each electrode 2 was discretized into six electrodes.

An electrode was set on the above-mentioned electrode 2 in various manners to calculate an S value, and an intermediate electrode (reference design of FIG. 14) was obtained as shown in FIG.

The S value of the electric field analysis result of FIG. 12 was calculated to be 172.8795.

Finally, the arrangement of the electrode (the optimized design of FIG. 14) showing the highest S value is shown in FIG. The S value at this electrode was calculated to be 243.4763.

Fig. 14 shows the results of E. coli collection efficiency calculated by computer software (Comsol) for the three electrodes. As shown in FIG. 14, it was confirmed that the collection efficiency and the S value showed the same tendency.

On the other hand, FIG. 15 shows the result of calculating the S value while increasing the number of grids per one electrode.

As shown in FIG. 15, when the number of grid lines is increased, the S value tends to increase. However, when the number of grid lines is 66 or more, the increase amount is insignificant. Are confirmed to be within the appropriate range.

That is, the number of the above-mentioned divergence grids is in a suitable range of at least 3 to 90 or less per one electrode.

Where S_r = S_optimized / S_conventional.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, And all of the various forms of embodiments that can be practiced without departing from the technical spirit.

1: channel 2: conventional electrode
3: Dioxide electrode
S1: Set-up type electrode step S2: Electrode dioxide step
S3: Calculation of electric field intensity Step S4: Calculation of final electrode

Claims (5)

A method for generating an electrode for a dielectrophoretic-based particle separation and collection device,
A plurality of linear electrodes arranged vertically in a channel length direction;
An electrode discretization step of disposing the plurality of linear electrodes horizontally in the channel length direction;
An electric field strength calculation step of calculating electric field intensities for each type of power source by setting various types of power sources to the discrete electrodes; And
And a final electrode calculation step of selecting an electrode shape of the maximum electric field intensity among the set power sources.
[2] The method of claim 1, wherein the electric field strength calculation step comprises a power setting step, an electrode connection step of connecting the electrodes to the set power state, and a factor calculation step of calculating the electric field strength based on the connected dioxide electrode A method for generating an electrode for a particle separation and collection device based on dielectrophoresis.
3. The method of claim 2, wherein the factor calculation step comprises: calculating an electric field intensity in the x direction (channel length direction) of the planar angle grid i with the connected dioxide electrode to be E xi and an electric field intensity in the y direction (vertical direction in the x direction) E yi , the factor of the grid i is E xi 2 + E yi 2 and the total factor S is the sum of the factors in all the grids for the connected dioxide electrode. A method for generating an electrode.
4. The method of claim 3, wherein the electrode shape having the highest total factor (S) is selected in the final electrode calculation step.
[6] The method of claim 4, wherein the electrode is discretized into three or more electrodes in the electrode discretization step.
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KR102072129B1 (en) 2019-07-16 2020-01-31 이혁기 Complex aerosol filter and filter assembly using the same

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KR100899138B1 (en) * 2007-07-05 2009-05-26 한국과학기술원 Device for Focusing and Seperating Micro Particles
KR101269168B1 (en) * 2011-06-02 2013-05-29 연세대학교 산학협력단 high efficiency particle separation apparatus and method

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Title
안재민 외. 대한기계학회 논문집 A권, 2009, Vol.33, No.1, pp,49-55.
윤길호 외. 대한기계학회 추계학술대회 강연 및 논문 초록집, 2009.11, pp.422-426.

Cited By (1)

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Publication number Priority date Publication date Assignee Title
KR102072129B1 (en) 2019-07-16 2020-01-31 이혁기 Complex aerosol filter and filter assembly using the same

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