KR101692969B1 - A composition for diagnosing active oxygen and a analyzing method using the same - Google Patents
A composition for diagnosing active oxygen and a analyzing method using the same Download PDFInfo
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- KR101692969B1 KR101692969B1 KR1020140088922A KR20140088922A KR101692969B1 KR 101692969 B1 KR101692969 B1 KR 101692969B1 KR 1020140088922 A KR1020140088922 A KR 1020140088922A KR 20140088922 A KR20140088922 A KR 20140088922A KR 101692969 B1 KR101692969 B1 KR 101692969B1
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
본 발명은 활성산소 진단용 조성물 및 활성산소 분석방법에 관한 것이다.The present invention relates to an active oxygen diagnostic composition and an active oxygen analysis method.
Description
본 발명은 활성산소 진단용 조성물 및 활성산소 분석방법에 관한 것이다.The present invention relates to an active oxygen diagnostic composition and an active oxygen analysis method.
활성산소는 반응성이 풍부한 산소로 동맥 경화가 원인인 심장병과 뇌졸중, 당뇨병이나 비만과 같은 성인병 그리고 암, 노화 등 대부분의 질병과 밀접하게 연관되어 있다고 최근 연구에서 밝혀지고 있다.Active oxygen is a reactive oxygen that has been shown in recent studies to be linked to heart disease caused by atherosclerosis, adult diseases such as stroke, diabetes and obesity, and most diseases such as cancer and aging.
이에 사람들은 질병에 걸리지 않고 건강을 유지하기 위해서 지금까지 알고 있었던 영양이나 건강에 대한 지식만으로는 충분하지 못하며, 활성산소와 그 해에 대한 지식을 가지고 가능한 한 체내에서 활성산소를 발생시키지 않도록 생활할 필요가 있다.In order to maintain health without getting sick, people do not have enough knowledge about nutrition and health that they have known so far. They have to live with the knowledge of active oxygen and its solution so as not to generate active oxygen in body as much as possible. .
또한 활성산소의 해를 예방하는 것뿐만 아니라 체내에 항산화제를 증가시키고 신체의 항산화 시스템 기능을 높이는 등 건강유지를 위한 적극적인 대책이 필요하다. 이러한 적극적인 대책을 위해 체내의 활성산소를 검사할 수 있는 분석 방법이 필요하다.In addition, it is necessary not only to prevent the harmful effects of active oxygen, but also to increase the antioxidant in the body and to enhance the function of the body's antioxidant system. For this active measure, an analytical method that can examine the active oxygen in the body is needed.
종래에는 이러한 활성산소를 분석하는 방법으로 상용화된 일례로서 혈액을 채취하여 1차반응시약을 투입 혼합한 뒤 원심분리기에 넣고 혈장과 혈구를 원심분리 한 후, 혈장과 혈구를 분리한 혈액에 2차반응시약을 투입 혼합한 다음 일정시간이 경과된 뒤 활성산소를 분석하게 된다.Conventionally, as a method for analyzing such active oxygen, for example, blood is collected, first reaction reagent is added and mixed, and centrifugal separation of plasma and blood cells is carried out in a centrifuge. Then, After the reaction reagent is added and mixed, active oxygen is analyzed after a certain time has elapsed.
종래 정확한 활성산소의 분석을 위해 활성산소 분석장치를 사용한 분석은, 혈액을 채취해야 하고 정해진 시간 동안 기다린 뒤에 반응시약을 투입하는 등 절차가 매우 복잡하여 일반인이 직접 활성산소를 측정하기에는 매우 곤란한 문제점이 있다.Conventionally, the analysis using the active oxygen analyzer for analyzing the accurate active oxygen has a problem that it is very difficult for the general person to directly measure the reactive oxygen because the procedure is very complicated such as taking the blood and putting the reagent after waiting for the predetermined time have.
또한 이러한 종래의 활성산소 분석을 위한 장치는 고가이고, 또 분석방법 또한 사용되는 시약과 절차가 복잡하여 시간과 비용이 많이 소요되는 문제점이 있다.Also, the conventional apparatus for analyzing active oxygen is expensive, and the analytical method is complicated because reagents and procedures are used.
[선행 특허 문헌][Prior Patent Literature]
대한민국 특허공개번호 제10-2006-0002769호Korean Patent Publication No. 10-2006-0002769
본 발명은 상기의 문제점을 해결하고, 상기의 필요성에 의하여 안출된 것으로서 본 발명의 목적은 신규한 간편하고 신속한 활성산소분석 조성물을 제공하는 것이다. DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a novel simple and rapid active oxygen analysis composition.
본 발명의 다른 목적은 신규한 간편하고 신속한 활성산소분석 방법을 제공하는 것이다. Another object of the present invention is to provide a novel simple and rapid active oxygen analysis method.
상기의 목적을 달성하기 위하여 본 발명은 a)메틸인돌을 이소프로판올에 녹인 후 산을 첨가하여 제조된 제1시약; 및 b)염기 조건에서 메틸레드가 혼합된 제2시약을 유효성분으로 포함하는 활성산소 진단용 조성물을 제공한다.In order to accomplish the above object, the present invention provides a method for preparing a polyimide resin, comprising: a) a first reagent prepared by dissolving methyl indole in isopropanol and then adding an acid; And b) a second reagent mixed with methyl red under basic conditions as an active ingredient.
또 본 발명은 상기 본 발명의 활성산소 진단용 조성물을 뇨에 첨가하여 색변화 차이를 통한 활성산소 진단 방법을 제공한다.In addition, the present invention provides a method for diagnosing active oxygen through color difference by adding the active oxygen diagnostic composition of the present invention to urine.
이하 본 발명을 설명한다.Hereinafter, the present invention will be described.
본 발명은 소변에 함유되어 있는 활성산소 부산물 측정용 시약 조성물로, 자세하게는 활성산소의 주 부산물인 알데히드 농도에 따른 변색 효율이 우수한 시약을 포함하는 조성물 및 이를 이용한 활성 산소 분석 방법에 관한 것이다.The present invention relates to a reagent composition for measuring active oxygen side products contained in urine, and more particularly, to a composition comprising a reagent excellent in discoloration efficiency according to aldehyde concentration, which is a main byproduct of active oxygen, and an active oxygen analysis method using the same.
본 발명을 통하여 알 수 있는 바와 같이, 본 발명의 조성물을 이용하여 10분 이내의 짧은 시간 안에도 원하는 결과를 확인해볼 수 있는 효과가 있다. As can be seen from the present invention, the desired result can be confirmed even within a short time of 10 minutes or less by using the composition of the present invention.
도 1은 본 발명의 조성물의 사용예를 나타낸 사진임. 1 is a photograph showing an example of use of the composition of the present invention.
이하 본 발명을 비한정적인 실시예를 통하여 상세하게 설명한다. 단 하기 실시예는 본 발명을 예시하기 위한 의도로 기재한 것으로서 본 발명의 범위는 하기 실시예에 의하여 제한되는 것으로 해석되지 아니한다.Hereinafter, the present invention will be described in detail by way of non-limiting examples. The following examples are intended to illustrate the present invention and the scope of the present invention is not to be construed as being limited by the following examples.
실시예Example
시약 1의 제조 및 특성Preparation and Characterization of Reagent 1
메틸인돌을 0.1M의 농도로 용매인 이소프로판올에 교반시켜서 완전히 녹인다. 여기에 2N 염산을 20% 부피비로 넣어 잘 섞는다. Methylindole is dissolved in isopropanol as a solvent at a concentration of 0.1 M to completely dissolve. Add 2N hydrochloric acid in a 20% volume ratio and mix well.
메틸인돌은 산성 조건에서 인돌이 양성자를 가지게 되고, 반응성이 높아져 알데히드와 강하게 반응한다. 알데히드와 결합한 메틸인돌 용액은 시간이 진할수록 점점 진한 주황색으로 바뀌게 된다. Methylindole has an indole as a proton in an acidic condition and reacts strongly with aldehyde because of its high reactivity. The methylindole solution combined with the aldehyde gradually turns dark orange with time.
시약 2의 제조 및 특성 Preparation and Characterization of Reagent 2
증류수로 0.0025N 수산화나트륨 수용액을 만든다. 여기에 메틸레드 시약을 4%의 비율로 잘 섞는다. Make 0.0025N aqueous sodium hydroxide solution as distilled water. The methyl red reagent is mixed well at a ratio of 4%.
시약2는 단순 지시약인 메틸레드를 매우 약한 농도로 희석한 용액으로, 소변과 시약1과의 반응을 빠른 시간 안에 눈으로 확인하기 위한 도움을 주는 용도로 사용되었다. 소변에 알데히드가 많이 없는 경우엔, 연한 핑크색을 갖다가 활성산소를 많이 포함한 소변에서는 주황색으로 변화하여 그 정도를 시각적으로 확인할 수 있게 하였다. Reagent 2 was a diluted solution of Methyl Red, a simple indicator, at a very weak concentration, and was used to help identify the reaction of urine with Reagent 1 in a short time. When there is not much aldehyde in the urine, it is changed to orange color in the urine containing a lot of active oxygen by light pink color, so that it can be visually confirmed.
적용례Application
상기 제조된 시약1 과 시약2를 1:1의 양으로 소변 샘플 10ml에 넣어 반응시킨다.Reagent 1 and Reagent 2 were reacted in 10 ml of urine sample in an amount of 1: 1.
개개인의 소변에 시약1을 넣고 반응시키면 실험실에서 길게는 30분 이상까지도 반응시켜야 개인의 활성산소 양에 따른 색변화의 차이를 볼 수가 있다. 이 경우에는 UV 흡광영역이 발생되어 수치화할 수도 있다. 그러나 이는 시간이 많이 걸리고, 실험실에서 진행되어야 하는 제약이 있게 된다. 그래서 시약 2를 넣어줌으로써, 10분 이내의 짧은 시간 안에도 원하는 결과를 확인해볼 수 있었다(도 1 참조).
If you add 1 reagent to your urine and react it, you can see the difference of color change according to the amount of active oxygen by reacting for longer than 30 minutes in the laboratory. In this case, a UV light absorption region may be generated to be quantified. However, this is time-consuming and there are restrictions that must be made in the laboratory. Thus, by adding reagent 2, the desired result can be confirmed within a short time of 10 minutes (see FIG. 1).
Claims (2)
b)염기 조건에서 메틸레드가 혼합된 제2시약을 유효성분으로 포함하고, 상기 제1시약과 제2시약의 혼합비는 1:1인 것을 특징으로 하는 활성산소 진단용 조성물.a) a first reagent prepared by dissolving methyl indole in isopropanol and then adding an acid; And
b) a second reagent in which methyl red is mixed in a basic condition as an active ingredient, and a mixing ratio of the first reagent and the second reagent is 1: 1.
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