KR101644054B1 - Nucleic acids and proteins separation device - Google Patents

Abstract

The present invention relates to nucleic acid and protein separation apparatus. The nucleic acid and protein separation apparatus according to an embodiment of the present invention comprises a separating and supporting unit provided with a sample in a container together with beads for pulverization and centrifugal separation, And is rotatable together with the separating and supporting part while adjusting the inclination angle so as to maintain the inclined state at the time of crushing, An angle adjusting unit disposed to rotate while maintaining a state parallel to the ground during centrifugal separation; an angle adjusting unit disposed at a lower portion of the separation supporting unit, wherein an elastic force acts in an upward direction on one side in a state where the separation supporting unit is rotatably supported, When the angle is adjusted by the angle adjusting part, one direction is shifted upward, A rotation supporting part disposed at a lower portion of the adjustment supporting part and connected to rotate the angle adjusting part; a rotation supporting part disposed at a lower part of the rotation part to move up and down to adjust the angle of the angle adjusting part; And a base portion installed to support the vibration of the angle adjusting portion, the rotating portion, and the up and down moving portion while absorbing vibrations.

Description

[0001] NUCLEIC ACID AND PROTEIN SEPARATION DEVICE [0002]

The present invention relates to a nucleic acid and protein separation apparatus, and more particularly, to a nucleic acid and protein separation apparatus, which collects sample samples for molecular diagnosis and genetic recombinant food inspection and performs functions of crushing and centrifuging The present invention relates to a nucleic acid and a protein separation apparatus capable of shortening the separation time by preventing the contamination due to the sample movement during the separation process.

In general, molecular diagnostics is a molecular biology technique to identify molecular defects that are difficult to solve in genetic diseases. It is possible to collect samples of blood, hair, saliva, semen, RNA) is separated and nucleic acid is extracted and tested. It is used to differentiate species, to diagnose the cause of a specific disease and to estimate the prognosis.

In addition, as the unique information of living things (DNA) is discovered, it has become possible to insert a useful gene of one organism into another organism to make a new variety. Is a technique for extracting a useful gene and artificially inserting it into another organism.

GMO foods are GMO foods made from GMO crops cultivated with new recombinant technology. GMO crops have a positive advantage in developing excellent varieties that are more resistant to pests and increase yields through genetic manipulation. However, there is a lack of validity regarding the stability of GMO foods and concerns about human health such as allergy and weakened immune systems There is a problem. In addition, there are risks of ecosystem disturbance and biodiversity destruction due to long-term cultivation of GMO crops, and various measures are being prepared for the management of GMO crops at national level.

However, since GMO crops can not be distinguished from other crops in the form and shape of the same shape and taste as the normally cultivated crops, the nucleic acid or protein can be identified by analyzing the sample, To conduct GMO testing.

The above-described methods for conducting the molecular diagnosis or the GMO test are generally carried out by collecting samples through animal cells, plant tissues, blood, cervical entrance, oral cavity, rice grains, corn kernels and the like, RNA) or protein is extracted and extracted and analyzed by electrophoresis or other analytical methods to diagnose the molecule or perform a test to identify GMO.

Here, a method for separating a nucleic acid and a protein from each other is a method in which a user manually grinds the sampled sample through a pestle in a state of being placed in a mortar, and the pulverized sample is moved to a centrifuge to be.

The method of separating the nucleic acid and the protein as described above has a problem in that the pulverization efficiency is lowered by performing the pulverization through manual operation and the possibility of the contamination of the sample due to the inflow of foreign matter during the pulverization is increased.

Recently, milling is carried out automatically using a homogenizer (homogenizer) that crushes the sample. Such a homogenizer is shaken at a high speed by putting a bead in the form of a small bead together with a sample in a container, and grinding is performed by mutual collision of cells and beads.

Such a method of separating nucleic acid and protein from a sample of the prior art using a homogenizer can be carried out by shaking the sample in the container through the homogenizer so that the sample in the container collides with the bead and then moving the pulverized sample to the centrifuge, There is a problem that the separation time is delayed as the nucleic acid and protein are separated by separation.

Further, when the container crushed in the homogenizer is moved to a centrifuge, there is a problem that the crushed sample is deteriorated or contaminated due to a change in the external temperature and delay of time.

Accordingly, there is a need for a technique for shortening the separation time while minimizing the occurrence of deterioration or contamination by integrating a pulverization and centrifugal separation device for separating nucleic acid and protein from the sample into one.

Disclosure of Invention Technical Problem [8] The present invention has been made to solve the above-mentioned problems, and an object of the present invention is to provide a method for analyzing a sample, The present invention provides a nucleic acid and protein separation device that shortens the time for separating nucleic acids and proteins from each other and separates nucleic acids and proteins without migration of the sample, thereby minimizing the possibility of deterioration and contamination, thereby improving reliability.

The technical problem of the present invention is not limited to those mentioned above, and another technical problem which is not mentioned can be clearly understood by those skilled in the art from the following description.

According to an aspect of the present invention, there is provided a nucleic acid and protein separation apparatus comprising: a separating and supporting unit provided rotatably in a state in which a plurality of containers are inserted and supported; And is rotatable together with the separating and supporting part while adjusting the inclination angle so as to maintain the inclined state at the time of pulverization and maintain a state parallel to the ground at the time of centrifugal separation And an elastic force is applied to one side of the separation supporting portion in a rotatable manner so that when the angle adjusting portion adjusts the angle by one angle, So that it is rotatably supported in an inclined state, A rotation unit disposed at a lower portion of the adjustment support unit and connected to rotate the angle adjustment unit, a vertical movement unit disposed at a lower portion of the rotation unit and configured to move up and down to adjust an angle of the angle adjustment unit, And a base portion configured to support and absorb vibration of the angle adjusting portion, the rotating portion, and the up and down moving portion.

The separating and supporting portion may include a separating bracket having a container inserting hole into which the container is inserted in a plurality of through holes at a radial position so as to be inserted and supported by the plurality of containers, A separating body having an upper portion and a lower portion opened in the inside and connected to the angle regulating portion by insertion so that the angle is adjusted and rotated together; and a cover body disposed on the upper portion of the separating body, And an upper cover provided to support the upper portion of the container supported by the separation bracket in a state where the angle adjusting portion is inserted and the upper portion is fixedly supported.

The angle adjusting portion is inserted into and supported by the separating and supporting portion. The angle adjusting portion is hinge-coupled to the lower portion so as to be inclined with the separating and supporting portion at an inclined angle when the upper and lower moving portions move up and down. And a separating and supporting unit that moves in a state where the separating and supporting unit moves in parallel with the ground during a downward movement of the adjusting and linking unit, An adjusting fixture disposed on an upper portion of the adjusting link body and having an upper portion of the adjusting link body inserted into the separating and supporting portion and having an enlarged sectional area so as to fix and support the upper portion of the separating and supporting portion, And the upper portion is hinged to the adjusting link body And a control shaft rotatably supported by the upper and lower moving parts so as to be moved up and down. The adjusting and rotating shaft is hinge-coupled to the adjusting and rotating shaft, The adjustment hinge is disposed at a center of the hinge hole and the shaft hinge hole, the adjustment hinge being disposed at a central position where the adjustment link member is angularly adjusted by rotation. The adjustment hinge is inserted at the center of the adjustment rotation shaft, A pair of upper and lower supporting members disposed on the lower portion of the adjusting and rotating shaft to be supported by the up and down moving unit and configured to move the adjusting and rotating shaft up and down by driving the up and down moving unit .

In addition, the adjustment support portion may include a control bracket disposed at a lower portion of the separation support portion and provided with the angle control portion rotatably supported, a plurality of protrusions protruding from a radial position of the control bracket, A support bar supporting the lower one side of the separating and supporting part in a state where the lower part is in contact with the separating and supporting part in a radial position and the separating and supporting part supporting the plurality of positions in a parallel state, A bearing support provided at one lower side position of the separating and supporting part which is moved upwards so as to be inclined, an upper supporting part disposed on the upper side of the bearing supporting part and supported to be vertically movable on the bearing supporting part, In the form of bearings that can be contacted and supported And an adjustable bearing disposed between the adjusting bracket and the adjustable bearing to provide an elastic force to move the adjustable bearing upward so that the angle adjuster is driven when the adjuster is moved upward by the operation of the up and down moving part, And a bearing elastic body provided so as to provide an urging force to provide an elastic force to the one side of the separating and supporting portion that is in contact with the separating and supporting portion and provide a tight contact force to be rotatably supported by the regulating bearing while the separating and supporting portion is parallel to the ground .

The rotation unit may include a rotary support disposed at a lower portion of the adjustment support unit and configured to support rotation of the angle adjustment unit, a rotary support disposed at a lower portion of the rotary support unit, A clutch bearing in which a key insertion groove is formed to be key-engaged with a rotating portion of the adjusting portion, and the angle adjusting portion that is key-engaged is configured to rotate only in one direction, a clutch bearing provided on the outer circumference of the clutch bearing, A support body having a control bearing disposed therein for rotatably supporting a rotating portion of the angle adjusting portion that is rotated by the rotary gear, , And is disposed on one side of the rotary gear A drive shaft connected to transmit a rotational force for rotating the drive gear, a drive shaft disposed at a lower portion of the drive gear and disposed at a lower portion of the drive gear, A drive motor provided to rotate the rotation gear provided to the drive shaft by being connected to the drive gear to be engaged with the drive gear, and a driving motor disposed below the rotation gear, And a rotation support frame provided in a plate shape to support the up and down movement unit.

The up and down moving unit is disposed at a lower portion of the rotating unit and includes a shaft insertion hole into which the rotating part of the rotating unit is inserted, and a cam projection protruding downward in part from the shaft insertion hole position is formed The rotation part is lowered at the time of passing the cam protrusion in a state that the upper part of the rotation part is in contact with the lower surface by the rotation, and when the cam part is out of the cam protrusion part, A cam driving shaft disposed in a lower direction at the center of the up and down cam and provided in a shaft shape for transmitting a rotational force for rotating the up and down cam, A cam drive motor installed to provide power for rotating the cam drive shaft, Is disposed at an upper side of the cam driving motor, it characterized in that the rotation part may include a motor installed in the fixed body so as to secure the cam drive motor.

The base portion may include a vibration absorbing frame disposed at a lower portion of the rotation portion and having a frame space that is a space spaced at a predetermined interval below the rotation portion supported by the angle adjusting portion and the up and down moving portion, A vibration absorber disposed between the vibration absorber frames and configured to absorb vibrations generated at the at least one rotation portion in the frame space; and a vibration absorber disposed at a lower portion of the vibration absorber frame, And a base frame provided at a position to be supported by the vibration absorbing frame and connected to and supported by the vibration absorbing frame.

The details of other embodiments are included in the detailed description and drawings.

According to the apparatus for separating nucleic acid and protein according to an embodiment of the present invention, when nucleic acid and protein are separated from a sample in order to carry out a molecular diagnosis or a recombinant food test, pulverization and centrifugation are simultaneously carried out in one apparatus, And the reliability can be improved by minimizing deterioration or contamination.

Further, in the nucleic acid and protein separation apparatus of the present invention, when the sample is crushed, the sample is rotated in an inclined state such that the sample and the bead are collided with each other while being accommodated in the container. By combining the two apparatuses in one unit by operating the grinding and centrifugal separating mechanism in such a manner that the rotation and the rotation in the inclined state and the rotation in the state parallel to the ground are performed in one apparatus, Can be saved.

The apparatus for separating nucleic acids and proteins according to the present invention comprises a container for holding a sample and a small bead in a rotatable state while being supported by a plurality of containers, The grinding speed and the degree of grinding can be controlled by controlling the inclination angle so that the grinding efficiency can be improved.

In addition, the nucleic acid and protein separation apparatus of the present invention is provided with a clutch bearing that rotates in one direction so as to suppress an acting force acting in a reverse rotation direction during rotation in a tilted state for sample crushing, So that the rotation efficiency can be improved.

In addition, the apparatus for separating nucleic acids and proteins according to the present invention comprises a structure in which an angle is adjusted during crushing and centrifugation, a vibration absorbing body is provided between a frame supporting the rotating structure and a frame supporting the ground, And absorbs vibration generated during high-speed rotation according to absorption, thereby preventing flow.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the description of the claims.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a front view showing a crushed state of a nucleic acid and protein separation apparatus according to an embodiment of the present invention; FIG.
FIG. 2 is a right-side outline view showing a state of pulverization in the nucleic acid and protein separating apparatus of FIG. 1; FIG.
FIG. 3 is a front view showing the centrifuged state of the nucleic acid and protein separation apparatus of FIG. 1; FIG.
FIG. 4 is a cross-sectional view showing the nucleic acid and protein separation apparatus of FIG. 1 in a pulverized state. FIG.
FIG. 5 is a partial cutaway front view showing the state of pulverization in the nucleic acid and protein separation apparatus of FIG. 1;
6 is a partial cutaway front view showing the state of centrifugation in the nucleic acid and protein separation apparatus of FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can easily carry out the present invention.

In the following description of the embodiments of the present invention, descriptions of techniques which are well known in the technical field of the present invention and are not directly related to the present invention will be omitted. This is for the sake of clarity of the present invention without omitting the unnecessary explanation.

For the same reason, some of the components in the drawings are exaggerated, omitted, or schematically illustrated. Also, the size of each component does not entirely reflect the actual size. In the drawings, the same or corresponding components are denoted by the same reference numerals.

Hereinafter, the present invention will be described with reference to the drawings for explaining a nucleic acid and protein separation apparatus according to embodiments of the present invention.

FIG. 1 is a front view of the nucleic acid and protein separation apparatus according to an embodiment of the present invention. FIG. 2 is a right side outline view showing the nucleic acid and protein separation apparatus of FIG. 1, FIG. 4 is a cross-sectional view showing the nucleic acid and protein separation apparatus of FIG. 1 in a pulverized state, and FIG. 5 is a cross-sectional view of the nucleic acid and protein separation apparatus of FIG. FIG. 6 is a partial cutaway front view showing the state of centrifugation in the nucleic acid and protein separation apparatus of FIG. 1; FIG.

Referring to FIGS. 1 to 6, an apparatus 100 for separating nucleic acids and proteins according to an embodiment of the present invention is a device for simultaneously performing pulverization and centrifugation to separate nucleic acids and proteins from a sample. The sample is inserted into and supported by the nucleic acid and protein separating apparatus 100 in a state of being housed in the container 10 together with beads in the form of a small bead in which they are crushed due to mutual collision, followed by pulverization and centrifugation to separate nucleic acids and proteins .

 The nucleic acid and protein separation apparatus 100 includes a separating and supporting unit 110, an angle regulating unit 120, an adjusting and supporting unit 130, a rotation unit 140, A moving part 150, and a base part 160. [

The separation supporting portion 110 includes a separating bracket 111, a separating body 113, and an upper cover 115 into which a plurality of containers 10 are inserted.

The separation bracket 111 has a container insertion hole 112 in which a plurality of containers 10 are inserted and supported and in which the container is inserted in a plurality of through holes at a radial position. The separating bracket 111 is provided in the form of a cylindrical plate, and is supported in a container insertion hole 112 formed at a plurality of radial positions with a container 10 in which a sample and a bead are housed together. The separating bracket 111 is moved to the inclined position after the container 10 is supported and then rotated. Then, the sample and the bead collide with each other in the container 10 while colliding with each other. After the crushing, the separating bracket 111 is moved to a position parallel to the ground, and then the crushed sample is centrifuged in the vessel 10.

The separating body 113 is disposed at the center of the separating bracket 111. The separating body 113 is formed with a separating space 114 through which the upper and lower portions are opened to insert the angle adjusting portion 120. In the separation space 114, an angle is adjusted together with the separation body 113, and the angle adjustment unit 120 is installed so as to be rotatably supported.

When the separating body 113 is moved at an inclined angle by the operation of the angle regulating part 120, the separating bracket 111 moves to the inclined position together with the rotating body 140, (Not shown).

On the other hand, during centrifugal separation, the centrifugal separator is moved together with the separation bracket 111 to a position parallel to the paper surface, and then rotated together with the rotation of the angle regulator 120. That is, the separating body 113 is provided at the center of the separating bracket 111 so that the angle regulating part 120 is inserted into the separating space 114 and rotated together with the separating bracket 111 while adjusting the angle do.

The upper cover 115 is disposed on the upper portion of the separating body 113 and has a through hole 116 through which the angle adjusting portion 120 is inserted. The upper cover 115 is provided so that the upper portion of the upper cover 115 is fixedly supported by the angle adjusting portion 120 inserted into the cover hole 116 while supporting the upper portion of the container 10.

The upper cover 115 is provided to contact and support an upper portion of a plurality of containers 10 inserted into and supported by the separation bracket 111 from the upper portion of the separation body 113. The upper cover 115 is installed to fix the upper part in a state where the angle adjusting part 120 is inserted into the cover hole 116 penetrating through the center. Accordingly, when the upper cover 115 covers the upper part of the container 10, (10) can be prevented from flowing.

The angle adjusting unit 120 includes an adjusting link member 121 for moving the separating bracket 111 to an inclined position, an adjusting fixing member 123, an adjusting rotating shaft 124, an adjusting hinge 126, And an upper and lower support member 129.

The adjusting link body 121 is inserted into and supported by the separating body 113 and moves downward at an inclined angle with the separating body 113 when the up and down moving unit 150 is moved up and down by the operation of the up and down moving unit 150 A hinge hole 122 in the form of a through hole is formed. The adjusting link body 121 moves the separating body 113 in an inclined manner about the hinge hole 122 when the upper linking unit 150 is moved upward by the operation of the up and down moving unit 150, And is provided in the form of a link moving in a parallel state. That is, the adjusting link member 121 is rotated at the time of up-and-down movement by the operation of the up-and-down moving unit 150 about the hinge hole 122 formed at the lower part, ). At this time, the separation bracket 111 moved together with the separation body 113 moves to a centrifugal separation position parallel to the ground at an inclined crushing position, and the angle can be adjusted.

The adjustment fixture 123 is disposed on the upper portion of the adjustment link member 121 and the adjustment fixture 123 is enlarged in cross section on the upper portion of the adjustment link member 121 inserted in the cover hole 116, (115). The adjustment fixture 123 is provided to fix the upper portion of the upper cover 115 supporting the upper portion of the container 10 to the adjustment link body 121. The adjusting fixture 123 fixes the upper part of the upper cover 115 to prevent the flow of the container 10 during rotation after the angle is adjusted or in parallel with the ground, 113) can be improved.

The adjusting rotation shaft 124 is disposed at a lower portion of the adjusting link body 121 and an axial hinge hole 125 is formed at an upper portion of the adjusting rotation shaft 124 to be hinged to the adjusting link body 121. A coupling key groove 127 is formed at a driving position of the rotation part 140 of the adjustment rotation shaft 124 so as to be inserted into the rotary part 140 in a keyed manner. The adjustment rotary shaft 125 is provided in the form of a shaft supported so as to be moved up and down by the operation of the vertical movement unit 150.

The adjustment rotation shaft 124 is vertically moved by the operation of the up and down movement unit 150 supported at the lower portion thereof. The adjustment linkage body 121 is hinged to adjust the angle of the adjustment linkage 121, As shown in Fig.

That is, when the up-and-down movement unit 150 is operated, the adjustment rotation axis 124 is moved up and down so that the hinge-coupled adjustment link member 121 is rotated and operated to adjust the angle. When the adjusting rotation shaft 124 is moved upward, the adjusting link member 121 is rotated and the separating bracket 111 is inclined to one side. When the adjusting link shaft 121 is moved downward, the separating bracket 111 is returned to its original position, To be moved in a parallel state.

The adjustment rotation shaft 124 is key-engaged with the rotation unit 140 so that when the adjustment rotation shaft 124 is rotated according to the operation of the rotation unit 140, the adjustment linkage 121 is hinged, 121 are rotated, the separating body 113 and the separating bracket 111 are rotated together. That is, at the time of grinding, the separation bracket 111 transmits the rotation force of the rotation unit 140 at the inclined position to the adjustment rotation axis 124 and rotates. When the separation bracket 111 is in parallel with the ground, (140) is transmitted from the adjustment rotary shaft (124) and rotated.

In other words, the rotation force of the rotation unit 140 is transmitted to the adjustment rotation axis 124 to rotate the separation body 113 and the separation bracket 111 together with the adjustment link body 121 to crush or centrifuge the sample in the vessel 10 Can be separated.

The adjusting hinge 126 hinges on the adjusting link 121 and the adjusting rotating shaft 124 and is inserted into the hinge hole 122 and the shaft hinge hole 125 respectively so that the adjusting link 121 is rotated And is installed at a position where the angle is adjusted.

The coupling key 128 is disposed at the center of the adjustment rotation shaft 124 and is provided to key-couple the adjustment rotation shaft 124 and the rotation unit 140. The engaging key 128 is inserted into the engaging key groove 127 of the regulating rotation shaft 124 and the rotating part 140 to be key-engaged. The coupling key 128 is provided to key-couple the adjustment rotation shaft 124 and the rotation unit 140 to transmit the rotation force of the rotation unit 140 to the adjustment rotation axis 124.

The upper and lower support members 129 are disposed at the lower portion of the adjustment rotary shaft 124 and are installed to be engaged with the vertical movement unit 150 so as to be enlarged in sectional area at the lower portion of the adjustment rotary shaft 124. The upper and lower support member 129 is vertically movable when the upper and lower movable members 150 are moved by the engagement and supported movement.

The upper and lower support member 129 is provided so as to have a larger cross sectional area at the lower portion of the adjustment rotary shaft 124 and is supported by the upper and lower movable unit 150 so that the adjustment rotary shaft 124 is moved up and down Respectively.

The adjustment support 130 includes an adjustment bracket 131 rotatably supporting the adjustment rotary shaft 124, a support bar 132, a bearing support 133, a control bearing 134, and a bearing elastic body 135 .

The adjustment bracket 131 is disposed at a lower portion of the separation body 113 and is installed with the adjustment rotation shaft 124 being rotatably supported therein.

A plurality of support bars 132 are provided at radial positions of the adjustment bracket 131 and the lower portion of the separation body 113 is supported to be contacted at radial positions. The support bars 132 protrude radially from the upper side of the adjustment bracket 131 and are supported to contact and support the lower part of the upper separation body 113. The supporting bar 132 supports the inclined lower side of the separating body 113 when the separating body 113 is tilted by the operation of the adjusting link 121. When the separating body 113 is kept parallel to the ground, As shown in Fig.

The bearing support 133 is disposed on one side of the adjustment bracket 131 so as to protrude upward and is installed at one lower position where the separation body 113 is moved upward so as to be inclined.

The adjustment bearing 134 is disposed on the upper portion of the bearing support 133 and is supported on the bearing support 133 so as to be movable up and down. The adjustment bearing 134 is in contact with the lower one side, And is provided in a bearing form capable of being supported. The adjustment bearing 134 is provided in the form of a bearing that is rotatably supported at a lower portion of the lower portion where the separate body 113 to be rotated is moved up to an inclined position. The adjustment bearing 134 is rotated in the direction in which the adjustment link body 121 is moved when the lower body is moved upward in a state in which the separation body 113 is rotatably supported at the time of upper movement by the operation of the up- So as to determine the direction in which the separating body 113 is inclined.

The bearing elastic body 135 is disposed between the adjustment bracket 131 and the adjustment bearing 134 and is provided to provide an elastic force such that the adjustment bearing 134 is moved upward.

When the pressing force is removed while the separating body 113 is moved upward by the operation of the up-down moving part 150, the elastic force of the bearing elastic body 135 continuously provides the elastic force of the adjusting bearing 135, As shown in Fig.

At this time, when the one side of the separating body 113 moves upward, the adjusting link body 121 is rotated about the adjusting hinge 126, and the separating body 113 is moved obliquely while moving one side of the separating body 113 upward .

The bearing elastic body 135 moves in a state in which the separating body 113 is parallel to the ground at the time of downward movement by the operation of the up and down moving part 150 and the control bearing 134 is moved by the elastic force of the separating body 113 So that the rotation support efficiency can be improved.

That is, the bearing elastic body 135 is installed to press the regulating bearing 134 upward by the resilient force, so that the resilient force for moving the lower side of the separating body 113 upward moves the regulating bearing 134 around the regulating hinge 126 To provide an action force to move the adjustment rotary shaft 124 in an upward direction by providing an action force that is rotated and moved in an inclined direction.

In other words, the bearing elastic body 135 provides an elastic force to move the separating body 113, which is moved together with the adjusting rotation shaft 124, in an inclined upward direction, It is installed so as to ascend to a tilted position by elasticity.

When the upper part of the separating body 113 is moved upward together with the adjusting link 121 by the operation of the up-down moving part 150 in the state where elastic force is applied to one side of the separating body 113, (121) is rotated around the adjusting hinge (126) so that one side of the separating body (113) moves upward and is kept tilted and crushed at the time of rotation.

When the adjusting link member 121 is moved downward by the operation of the up-down moving unit 150, the adjusting link member 121 and the adjusting rotating shaft 124 are in a straight line so that the separating body 113 is parallel to the ground So that the adjustment bearing 134 is kept in a pressurized state at one side of the separation body 113 to support the rotation.

The adjustment bearing 134 is moved upward by the elastic force of the bearing elastic body 135 when the upper movable part 150 is moved upward by the operation of the vertical movement part 150 so that the adjustment link body 121 rotates about the adjustment hinge 126 And is rotated and moved to a tilted position. When the lower part of the separating body 113 is pressed down by the operation of the up-down moving part 150, while the lower part of the separating body 113 is pressed, the regulating bearing 134 is kept in close contact with the separating body by the elastic force of the elastic body 135 So as to support the rotation of the separation body 113.

The rotary unit 140 includes a rotary support 141 provided at a lower portion of the adjustment bracket 131, a clutch bearing 142, a rotary gear 143, a support body 144, a drive gear 146, a drive shaft 147, A motor 148, and a rotation support frame 149.

The rotary support 141 is disposed under the adjustment bracket 131 and is installed to support the rotation of the adjustment rotary shaft 124 therein.

The clutch bearing 142 is disposed at the lower portion of the rotary support 141 and is inserted into the inner surface of the clutch bearing 142 in a slot shape movable upward and downward to be key- And the key-fitted adjustment rotary shaft 124 is installed so as to rotate only in one direction. The clutch bearing 142 restricts the rotation direction of the clutch bearing 142 in one direction so as to prevent the rotation of the clutch bearing 142 in the other direction so that the adjustment rotation shaft 124 that is key- And is prevented from being reversely rotated.

That is, the adjustment rotary shaft 124, which rotates the separation supporting portion 110 moved to the inclined position for grinding, is rotated in a state of being eccentrically rotated in one direction . In this way, when the rotation center is eccentric in one direction and the continuous rotation progresses, an action force is generated that is rotated in a direction opposite to the direction in which the centrifugal force is rotated out of the normal rotation direction. That is, if the separation supporting portion 110 is rotated in an inclined state for crushing, the rotation of the adjustment rotation axis 124 does not normally rotate in the forward direction, but idles due to the force acting in the reverse direction. .

Accordingly, a clutch bearing (142) for limiting the rotation direction so as to rotate the adjustment rotary shaft (124) in only one direction is provided to suppress the action of the reverse rotation force, so that the grinding operation is performed while maintaining the inclined state at a constant speed Whereby the crushing efficiency can be improved.

The key insertion groove 142a in which the engaging key is inserted into the inner surface of the clutch bearing 142 is moved by the operation of the up and down moving part 150 to move the adjusting rotation shaft 124 inserted with the engaging key 128 up and down And the adjustment rotary shaft 124 can be moved up and down while maintaining the key engagement state.

The rotary gear 143 is provided on the outer circumference of the clutch bearing 142 and is fixed to rotate the clutch bearing 142 by forming a gear on the outer surface thereof. The rotation gear 143 is fixedly installed to rotate the adjustment rotation shaft 124 which is key-engaged in the clutch bearing 142.

The support body 144 is disposed at a lower portion of the rotary gear 143 and is provided therein with an adjustment bearing 134 for rotatably supporting the adjustment rotary shaft 124 rotated by the rotary gear 143. The support body 144 is provided with an adjustable bearing 134 for supporting the rotation of the adjustment rotary shaft 124 rotated by the rotary gear from the lower side.

The drive gear 146 is disposed on one side of the rotary gear 143 and is gear-engaged to transmit the rotational power to one side of the rotary gear 143. The driving gear 146 is provided in the form of a gear that is gear engaged to rotate the rotary gear 143 and is installed to transmit the rotational force of the driving motor 148 to the rotary gear 143.

The drive shaft 146 is disposed in a downward direction from the center of the drive gear 146 and is connected to transmit rotational force to rotate the drive gear 146. The drive shaft 147 is installed to rotate the drive gear 146 while being rotated by the operation of the drive motor 148 in the form of an axis.

The driving motor 148 is disposed at the lower portion of the driving gear 146 and rotates the rotating gear 143 that is engaged with the driving gear 146 by providing rotational force to the driving shaft 147 connected to the driving gear 146. . When the drive gear 146 is rotated by the operation of the drive motor 148, the gear 143 coupled to the gear is rotated, and the clutch bearing 142 is rotated to restrict the rotation direction in one direction, (124) is rotated.

The rotary support frame 149 is disposed at a lower portion of the rotary gear 143 and is provided in a plate shape so as to support the support body 144, the drive shaft 147, the drive motor 148, and the up- do. The rotation support frame 149 supports both the structure in which the separation support portion 110 is supported to rotate the adjustment rotation shaft 124 and the structure in which the up and down movement portions 150 for vertically moving are supported, .

The vertical movement unit 150 includes a vertical movement cam 151 for vertically moving the adjustment rotary shaft 124 by rotation at a lower portion of the rotary support frame 149, a cam drive shaft 154, a cam drive motor 155, And a motor fixture 156.

The vertical movement cam 151 is disposed below the rotary support frame 149 and a shaft insertion hole 152 into which the adjustment rotary shaft 124 is inserted is formed inside the vertical movement cam 151. A cam protrusion 153 is formed at a lower portion of the up-and-down moving cam 151, the cam protrusion 153 partially protruding from the position of the shaft inserting hole 152. The upper and lower moving cam 151 is rotated by the rotation of the cam protrusion 153 in a state in which the upper and lower supporting members 129 are inserted into the shaft insertion hole 152 and the adjustment rotary shaft 124 is inserted, The adjusting rotary shaft 124 is lowered. When the upper and lower support member 129 moves away from the cam projection 153, the adjustment rotation shaft 124 is raised by the elastic force of the bearing elastic body 135. Therefore, the up-and-down moving cam 151 is provided so that the upper and lower support members 129 are allowed to move through the cam protrusion 153 by the rotation to move the adjusting rotation shaft 124 up and down.

The vertical movement cam 151 is rotatably supported by the shaft insertion hole 152 so that the upper and lower support members 129 fixed to the lower portion of the adjustment rotation shaft 124, which is elastically biased in the upward direction inclined by the bearing elastic member 135, The adjustment rotary shaft 124 is inserted. When the up-and-down moving cam 151 rotates, the upper and lower support members 129 reach the cam protrusion 153 protruding downward and move downward to be lowered so as to maintain the separation bracket 111 parallel to the paper. When the upper and lower support members 129 are moved out of the cam protrusion 153 by the rotation of the vertical movement cam 151, the elastic force of the bearing elastic member 135 in the sloping upward direction acts to raise the adjustment rotary shaft 124, Is moved to an inclined position.

The cam drive shaft 154 is disposed in a downward direction at the center of the vertical movement cam 151 and is provided in the form of a shaft for transmitting a rotational force for rotating the vertical movement cam 151.

The cam drive motor 155 is disposed below the cam drive shaft 154 and is provided to provide power for rotating the cam drive shaft 154. The cam drive motor 155 is installed to rotate the up-and-down movement cam 151 that moves the adjustment rotation shaft 124 up and down through the cam drive shaft 154 by operation.

The motor fixing body 156 is disposed on the upper portion of the cam drive motor 155 and is provided to fix the cam drive motor 155 to the rotation support frame 149. The motor fixing body 156 is installed such that the cam driving motor 155 is fixed to the rotary support frame 149 on which the adjustment rotary shaft 124 is supported.

The base portion 160 includes a vibration absorbing frame 161, a vibration absorbing body 163, and a base frame 164 installed to absorb vibration at a lower portion of the rotary support frame 149.

The vibration absorbing frame 161 is disposed at a lower portion of the rotary support frame 149. The vibration absorbing frame 161 is disposed at a position where the frame space 162, which is a space apart from the rotary support frame 149, Respectively. That is, the vibration absorbing frame 161 is installed at a position having a frame space 162, which is a space spaced apart at predetermined intervals, below the rotation supporting frame 149 on which the adjusting rotary shaft 124 and the motor fixing body 156 are supported do.

The vibration absorber 163 is disposed between the rotary support frame 149 and the vibration absorbing frame 161 and is installed to absorb vibration generated in the rotary support frame 149 at least in the frame space 162 . The vibration absorbing member 163 is made of at least one material in which vibration is absorbed between the vibration absorbing frames 161 in order to absorb the vibration of the rotary support frame 149, So as to absorb the vibration generated when the angle is adjusted, rotated, and moved up and down, thereby minimizing the flow. Here, the vibration absorbing member 163 is made of a urethane material that absorbs vibrations, but this is for convenience of explanation, and it is apparent to those skilled in the art that any material capable of absorbing vibration can be used.

The base frame 164 is disposed at a lower portion of the vibration absorbing frame 161 and is disposed at a position below the vibration absorbing frame 161 so as to be supported on the ground surface to support the vibration absorbing frame 161 and the frame connecting body 165 And is connected to be supported.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, And is not intended to limit the scope of the invention. It is to be understood by those skilled in the art that other modifications based on the technical idea of the present invention are possible in addition to the embodiments disclosed herein.

Description of the Related Art
10: vessel 100: separator
110: separation supporting part 111: separation bracket
112: container insertion hole 113: separation body
114: separating space 115: upper cover
116: cover hole 120: angle adjusting portion
121: Adjustable link body 122: Hinge ball
123: Adjusting fixture 124: Adjustable rotary shaft
125: Axial hinge ball 126: Adjustable hinge
127: coupling keyway 128: coupling key
129: upper and lower support body 130:
131: Adjusting bracket 132: Support bar
133: Bearing support 134: Adjustable bearing
135: bearing elastic body 140:
141: rotating support 142: clutch bearing
142a: key insertion groove 143: rotary gear
144: Support body 145: Adjustable bearing
146: drive gear 147: drive shaft
148: drive motor 149: rotary support frame
150: Up and down moving part 151: Up and down moving cam
152: shaft insertion hole 153: cam protrusion
154: cam drive shaft 155: cam drive motor
156: motor fixing body 160: base portion
161: Vibration absorption frame 162: Frame space
163: Vibration absorber 164: Base frame
165: frame connector

Claims (7)

A separation supporting portion 110 provided rotatably in a state where a plurality of containers 10 are inserted and supported;
And is rotatable together with the separating and supporting unit 110 while being adjusted to the angle of inclination by being diffracted in the form of a link according to the upward and downward movements, And an angle adjusting unit 120 installed to rotate while maintaining a state parallel to the ground during centrifugal separation.
In the state where the separation supporting portion 110 is rotatably supported, an elastic force acts on one side in the upward direction, and when the angle is adjusted by the angle adjusting portion 120, A control support 130 installed to be rotatably supported in a tilted state;
A rotation unit 140 disposed below the adjustment support unit 130 and connected to rotate the angle adjustment unit 120;
A vertical movement part 150 disposed below the rotation part 140 and installed to move up and down to adjust the angle of the angle adjustment part 120; And
And a base unit 160 installed to support and absorb vibration of the angle adjusting unit 120, the rotation unit 140, and the up and down moving unit 150,
The adjustment support part 130 is disposed at a lower portion of the separation support part 110 and includes an adjustment bracket 131 installed in a state where the angle adjustment part 120 is rotatably supported.
A plurality of protrusions are provided at radial positions of the control bracket 131 and the lower portion of the separation supporting portion 110 is contacted at a radial position so as to support a lower one side in a state where the separation supporting portion 110 is inclined, A support bar 132 installed to support a plurality of positions of the separation supporting portion 110 in a parallel state;
A bearing support 133 disposed on one side of the adjustment bracket 131 and projecting upwardly and installed at one lower position which is moved upward to be inclined by the separation support 110;
The bearing support body 133 is supported on the bearing support body 133 so as to be movable up and down and is in the form of a bearing capable of being rotatably supported by a lower one side of the separation support unit 110 An adjustable bearing 134; And
The adjustment bearing 134 is disposed between the adjustment bracket 131 and the adjustment bearing 134 to provide an elastic force to move the adjustment bearing 134 in the upward direction, The one side of the separation supporting part 110 which is in contact with the regulation bearing 134 is raised so that the regulating part 120 is driven so that the inclined supporting part 110 provides an elastic force and the separating supporting part 110 is parallel to the ground And a bearing elastic body (135) installed to provide an urging force to be closely contacted to be rotatably supported by the adjustment bearing (134)
The vertical movement part 150 is disposed at a lower portion of the rotation part 140 and includes a shaft insertion hole 152 into which the rotation part of the rotation part 140 is inserted, A cam protrusion 153 protruding in a downward direction from the cam protrusion 153 is formed on the lower surface of the cam protrusion 153. When the cam protrusion 153 passes through the lower surface of the cam protrusion 153, When the cam 140 protrudes from the cam protrusion 153 and the rotation part 140 moves up and down due to the elastic force of the adjustment support part 130, (151);
A cam driving shaft 147 disposed in a lower direction at the center of the up-and-down moving cam 151 and having a shaft shape for transmitting a rotating force for rotating the up-and-down moving cam 151;
A cam driving motor 148 disposed below the cam driving shaft 147 and provided to provide power for rotating the cam driving shaft 147; And
And a motor fixing body (156) disposed above the cam driving motor (148) and adapted to fix the cam driving motor (148) to the rotating part (140) .
The method according to claim 1,
The separating and supporting unit 110 includes:
A separation bracket (111) having a container insertion hole (112) in which a plurality of the containers (10) are inserted and supported and into which the container (10) is inserted in a plurality of through holes at a radial position;
The separation bracket 111 is disposed at the center of the separation bracket 111. The separation bracket 111 has an upper portion and a lower portion opened to connect the angle adjusting portion 120, (113); And
The angle adjusting part 120 is inserted into a cover hole 116 penetrating the center of the separating body 113 and is supported by the separating bracket 111 in a state where the upper part is fixedly supported. And an upper cover (115) adapted to support an upper portion of the container (10).
The method according to claim 1,
The angle adjusting unit 120 may adjust
And is inserted and supported in the separating and supporting part 110. The upper and lower moving parts 150 are hinge-coupled to the lower part to be inclined with the separating and supporting part 110 at an inclined angle when the upper and lower moving parts 150 are moved up and down. The hinge hole 122 is formed so that the separation supporting portion 110 is inclinedly moved about the hinge hole 122 when the upper moving portion 150 is moved upward, An adjusting link body 121 provided in a link shape in which the supporting portion 110 is moved in parallel with the ground;
The upper portion of the control link body 121 is enlarged in cross section on the upper portion of the control link body 121 inserted in the separation support portion 110 so that the upper portion of the separation support portion 110 is fixedly supported. An installed conditioning fixture 123;
A shaft hinge hole 125 is formed in the lower portion of the adjusting link member 121 so that an upper portion of the adjusting link member 121 is hinged to the adjusting link member 121, An adjusting rotation shaft 124 having a coupling key groove 127 formed therein and supported to be moved up and down by the operation of the up and down moving unit 150;
The adjustment link body 121 and the adjustment rotation shaft 124 are hinged to each other and inserted into the hinge hole 122 and the shaft hinge hole 125 so that the adjustment link body 121 is rotated An adjustable hinge 126 disposed at a central position where adjustment is made;
A coupling key 128 disposed at the center of the adjustment rotation shaft 124 and inserted into the coupling key groove 127 to be in key engagement with the rotation part 140; And
The upper and lower support member 150 is disposed at a lower portion of the adjustment rotation shaft 124 and is supported by the up and down movement unit 150 so that the adjustment rotation axis 124 is moved up and down by driving the up and down movement unit 150. (129). ≪ / RTI >
The method according to claim 1,
The rotation unit 140 includes:
A rotation support 141 disposed below the adjustment support 130 to support rotation of the angle adjustment unit 120;
A key insertion groove 142a is formed in the lower portion of the rotary support 141 and is in the form of an elongated hole that can be moved up and down on the inner surface to be engaged with the rotated portion of the angle adjusting portion 120, A clutch bearing 142 installed to rotate the angle adjusting unit 120 in only one direction;
A rotary gear 143 provided on an outer periphery of the clutch bearing 142 and having a gear formed on an outer surface thereof and fixed to rotate the clutch bearing 142;
And a support body 134 disposed at a lower portion of the rotary gear 143 and having an adjustable bearing 134 therein for rotatably supporting a rotating portion of the angle adjuster 120 rotated by the rotary gear 143 144);
A driving gear 146 disposed at one side of the rotary gear 143 and gear-coupled to transmit power to be rotated to one side of the rotary gear 143;
A driving shaft 147 disposed at a center of the driving gear 146 in a downward direction and connected to transmit rotational force to rotate the driving gear 146;
The rotation gear 143 is disposed below the driving gear 146 and rotatably supports the driving gear 146 coupled to the driving gear 146 by providing a driving force to the driving shaft 147 connected to the driving gear 146. A drive motor 148 installed to rotate the motor; And
The rotation gear 143 is disposed below the rotation gear 143 and rotates in a plate shape to support the support body 144, the driving shaft 147, the driving motor 148, And a support frame (149).
The method according to claim 1,
The base unit 160 includes:
A vibration space having a frame space which is a space spaced at predetermined intervals below the rotation unit 140 supported by the angle control unit 120 and the up and down movement unit 150, An absorption frame 161;
A vibration absorber 163 disposed between the rotation unit 140 and the vibration absorber frame 161 and adapted to absorb vibrations generated by the rotation unit 140 in at least one space in the space of the vibration absorber frame 161, ; And
And is disposed at a lower position of the vibration absorbing frame 161 and is supported at the bottom of the vibration absorbing frame 161 so as to be connected and supported by the vibration absorbing frame 161 and the frame coupling body 165. [ And an installed base frame (164). delete delete

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