KR101611850B1 - - Method for the extraction of -glucan from Lentinula edodes with high yield - Google Patents

- Method for the extraction of -glucan from Lentinula edodes with high yield Download PDF

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KR101611850B1
KR101611850B1 KR1020140063083A KR20140063083A KR101611850B1 KR 101611850 B1 KR101611850 B1 KR 101611850B1 KR 1020140063083 A KR1020140063083 A KR 1020140063083A KR 20140063083 A KR20140063083 A KR 20140063083A KR 101611850 B1 KR101611850 B1 KR 101611850B1
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beta
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shiitake
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이종성
옥주안
박효진
이균희
이윤복
오승현
이장운
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Abstract

본 발명은 열수 추출 및 초고압 추출을 순차적으로 병행하여 표고버섯으로부터 베타-글루칸을 고수율로 추출할 수 있는 방법에 관한 것으로, 단순 열수 추출 또는 단순 초고압 추출에 비해, β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸의 추출 수율이 약 20~30% 정도 향상되는 효과가 나타났다. The present invention relates to a method for extracting beta-glucan from a shiitake mushroom in high yield by sequentially performing hot water extraction and ultra-high pressure extraction, and more particularly, to a method for extracting beta-glucan from a mushroom, 1,3-linked beta-glucan was improved by about 20 ~ 30%.

Description

표고버섯으로부터 베타-글루칸을 고수율로 추출하는 방법 {Method for the extraction of β-glucan from Lentinula edodes with high yield}Method for Extracting Beta-Glucan from Shiitake Mushrooms in High Yield [0002]

본 발명은 표고버섯으로부터 베타-글루칸(β-glucan)을 추출하는 방법에 관한 것으로, 더욱 상세하게는, 열수 추출 및 초고압 추출을 순차적으로 병행하여 표고버섯으로부터 베타-글루칸을 고수율로 추출할 수 있는 방법에 관한 것이다.
The present invention relates to a method for extracting beta-glucan from shiitake mushroom, and more particularly, to a method for extracting beta-glucan from shiitake mushroom in succession in parallel with hot water extraction and ultra high pressure extraction How to do it.

베타-글루칸(β-glucan)은 다시마 등의 조류, 버섯, 효모 및 곡물 등에 있는 다당류 일종으로 세포막을 구성하는 성분이다. 베타-글루칸은 포도당 중합체인데, 포도당의 1, 3 위치에 β-글리코시드 결합을 기본 구조로 가지며, 이 외에 포도당이 결합되는 위치에 따라 β-1,4 또는 β-1,6의 구조를 가질 수 있다.Beta-glucan is a kind of polysaccharide found in algae, mushroom, yeast, and grains such as sea tangle, and constitutes a cell membrane. Beta-glucan is a glucose polymer, which has a basic structure of β-glycoside bonds at 1 and 3 positions of glucose, and has a structure of β-1,4 or β-1,6 depending on the position where glucose is bonded .

베타-글루칸은 β-1,3 결합, β-1,4 결합, β-1,6 결합 등 포도당 결합 위치 또는 분지 위치에 따라 종류가 다양한데, 기원에 따라서도 분지도, 용해도, 3차 구조 등에 많은 차이가 있어, 물리화학적 특성 및 생체기능이 다양한 것으로 알려져 있다 (E. Sonck et al., Veterinary Immunology and Immunopathology 135:199-207, 2010). 예를 들어, 다시마 유래의 베타-글루칸은 β-1,3 결합 및 β-1,6 결합(30:1)을 가지며 분자량이 약 7.7 kDa 이고, 표고버섯(Lentinus edodes)의 베타-글루칸은 β-1,3 주당쇄의 매 5개의 잔기 내에 2개씩 β-1,6-잔기를 갖는 β-1,6-분지-β-1,3-글루칸이며, 효모(Saccharomyces cerevisiae)의 베타-글루칸은 세포벽에서 추출되며, 분지가 거의 없고 불균일하며 불용성인 특징이 있다.Beta-glucan has various types depending on the position of glucose bonding or branching position such as β-1,3 bond, β-1,4 bond, β-1,6 bond, etc. Depending on the origin, degree of branching, solubility, tertiary structure It is known that there are many differences in physico-chemical properties and biological functions (E. Sonck et al., Veterinary Immunology and Immunopathology 135: 199-207, 2010). For example, beta-glucan derived from kelp has a beta-1,3 bond and a beta-1,6 bond (30: 1) and has a molecular weight of about 7.7 kDa, and mushroom ( Lentinus beta edodes) - glucan is a β-1,6- branched -β-1,3- glucan, with a two by two residues in the β-1,6- every five residues of the β-1,3 week sugar, yeast ( Saccharomyces The β-glucan of S. cerevisiae is extracted from the cell wall and has little branching, heterogeneity and insolubility.

베타-글루칸은 면역력을 증가시키고 세포의 활성을 증가시키는 물질로 알려져 있는데, 최근의 연구 보고에 의하면, 항암 효과, 항당뇨 효과, 항고지혈증 효과, 항비만 효과 등이 있는 것으로 알려져 있다.Beta-glucan is known as a substance that increases immunity and increases the activity of cells. According to recent research reports, it is known that there is anticancer effect, antidiabetic effect, antihyperlipidemic effect and anti-obesity effect.

한편, 표고버섯(Lentinula edodes)은 담자균류 주름버섯목 느타리과에 속하는 버섯으로서, 영양기관인 균사체와 번식기관인 포자를 지닌 자실체로 되어 있고, 뒷면이 하얗고, 깨끗한 주름이 있으며, 특별한 향미와 감칠맛이 있어 예로부터 식용으로 널리 이용된 버섯이다.Meanwhile, shiitake mushrooms ( Lentinula edodes ) is a mushroom belonging to the family Aspergillus spp., which is a fruiting body with mycelium and reproductive organs as reproductive organs. It has white back, clean wrinkles, and has a special flavor and rich taste. It is mushroom.

표고버섯에는 단백질, 지방, 탄수화물뿐만 아니라 칼슘, 칼륨, 인, 셀레늄,철분 등의 미네랄, B1, B2 등의 비타민, 식이섬유가 풍부하게 함유되어 있다. 또한, 프로비타민 D(provitamin D)인 에르고스테롤(ergosterol), 혈압 강하와 당뇨병에 효과적인 에리타데닌(erithadenine), 탄수화물ㆍ지방ㆍ단백질 대사 및 세포호흡과 당 분해작용에 관여하는 나이아신(niacin) 등 생리활성물질이 있어 식용 외에 약재로도 이용되었다.Shiitake mushrooms contain not only proteins, fats and carbohydrates, but also minerals such as calcium, potassium, phosphorus, selenium and iron, vitamins such as B1 and B2, and dietary fiber. It is also known that ergosterol, which is provitamin D, erithadenine, which is effective for blood pressure lowering and diabetes, carbohydrate, fat and protein metabolism, and niacin, which is involved in cell respiration and glucose degradation It was also used as a medicinal material in addition to edible because of its physiologically active substance.

한편, 표고버섯에는 베타-글루칸이 많이 함유되어 있는 것으로 알려져 있는데, 표고버섯으로부터 베타-글루칸을 추출하고자 하는 노력들이 종래에 많이 이루어져 왔다. 하지만, 대부분 단순 가압 열수 추출에 의존하고 있어, 추출 수율이 그다지 높지 않은 실정이다. On the other hand, shiitake has been known to contain a large amount of beta-glucan. Efforts to extract beta-glucan from shiitake have been made in the past. However, most of them depend on the simple pressurized hot water extraction, and the extraction yield is not so high.

따라서, 표고버섯으로부터 베타-글루칸을 고수율로 추출할 수 있는 새로운 기술을 개발할 필요가 있다.
Therefore, it is necessary to develop a new technique for extracting beta-glucan from shiitake at a high yield.

표고버섯으로부터 베타-글루칸을 추출하는 방법에 관한 특허문헌으로는, 비록 최종 등록 거절된 것이지만, 대한민국 특허공개번호 제10-2004-0090374호 (공개일자 2004년 10월 22일)가 있다. 이 특허문헌에는, "건조된 표고버섯(Letinus edodes) 자실체로부터 알카리를 이용하여 추출물을 수득하는 1단계, 상기 추출물을 산용액으로 중화시키고 생성된 단백질 및 기타 불용성분의 침전을 제거하는 2단계, 탈염 및 농축시키는 3단계, 초한외여과 장치를 이용하여 재농축 및 불순물을 제거하여, 분자량 1만 이상의 베타-글루칸을 함유하는 수용성 다당체 용액을 분리 정제하는 4단계, 상기에서 수득된 수용성 다당체 용액을 동결건조하는 5단계를 포함하는 분자량 1만 이상의 베타-글루칸을 함유하는 고분자의 수용성 다당체의 분리 정제 방법"이 기재되어 있다.Patent literature on a method for extracting beta-glucan from mushrooms has been disclosed in Korean Patent Publication No. 10-2004-0090374 (published on October 22, 2004) although the final registration has been rejected. In this patent document, "a step of obtaining an extract from dried letitious mushroom (Letinus edodes) fruiting body by using alkali, a step of neutralizing the extract with an acid solution and a step of removing the precipitated protein and other insoluble components, Removing and concentrating the water-soluble polysaccharide solution obtained in the above step (4) to remove and concentrate the water-soluble polysaccharide solution containing the beta-glucan having a molecular weight of 10,000 or more by re- A method of separating and purifying a water-soluble polysaccharide of a polymer containing beta-glucan having a molecular weight of 10,000 or more and containing 5 steps of lyophilization ".

본 발명에서는 표고버섯으로부터 베타-글루칸을 고수율로 생산할 수 있는 새로운 추출 방법을 개발하여 제공하고자 한다.
In the present invention, a new extraction method capable of producing beta-glucan at a high yield from shiitake is developed and provided.

본 발명은 슬라이스된 표고버섯에 100~120℃의 열수를 표고버섯 중량의 15~25배만큼 첨가하고 1.00~1.5 kgf/cm2의 압력을 가하여 2~4시간 동안 열수 추출하는 단계(a); 상기 단계(a) 후, 온도를 50~70℃로 조정하고, 90~110 MPa의 초고압을 가하여 2~4시간 동안 초고압 추출하는 단계(b);를 포함하는 것을 특징으로 하는 표고버섯으로부터 베타-글루칸을 추출하는 방법을 제공한다. (A) adding hot water at a temperature of 100 to 120 ° C. to sliced mushroom at a rate of 15 to 25 times the weight of mushroom and extracting the mushroom at a pressure of 1.00 to 1.5 kgf / cm 2 for 2 to 4 hours; (B) a step (b) of adjusting the temperature to 50 to 70 ° C and extracting the mixture at an ultra-high pressure of 90 to 110 MPa for 2 to 4 hours after the step (a) A method of extracting glucan is provided.

상기 본 발명의 방법에 의할 경우, 베타-글루칸, 특히 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸을, '단순 가압 열수 추출' 또는 '단순 초고압 추출'에 비해 20~30% 높은 수율로 생산할 수 있다. 구체적으로, β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸이 단순 가압 열수 추출의 경우, 9.17% 함량으로 추출되고, 단순 초고압 추출의 경우, 9.73% 함량으로 추출됨에 반해, 본 발명의 '가압 열수 추출 → 초고압 추출'의 경우, 13.15%의 함량으로 추출되었다. 이때, 가압 열수 추출과 초고압 추출의 순서를 바꾼 '초고압 추출 → 가압 열수 추출'의 경우는, 10.97% 함량으로 추출되었다. According to the method of the present invention, beta-glucan, especially beta-glucan having beta-1, 6-branch beta-1,3 bond, It can be produced at a yield of 30%. Specifically, beta-glucan having beta-1, 6-branching beta-1,3 linkages is extracted with 9.17% in the case of simple pressurized hot water extraction and 9.73% in the case of simple hyperbaric extraction. In the case of the "hot pressurized hydrothermal extraction → ultra-high pressure extraction" of the invention, the content was extracted at a content of 13.15%. At this time, in case of 'Extra-high pressure extraction → Pressurized hot water extraction' in which the order of pressurized hot water extraction and ultra high pressure extraction was changed, the content was extracted to 10.97%.

한편, 본 발명의 표고버섯으로부터 베타-글루칸을 추출하는 방법은, 단계(b) 후, 동결건조하는 단계(c)를 추가로 포함할 수 있다. 동결건조는 당업계에 널리 알려진 방법을 어느 것이나 사용하여도 무방하다. On the other hand, the method for extracting beta-glucan from the shiitake of the present invention may further include a step (c) of lyophilizing after step (b). The lyophilization may be carried out by any method well known in the art.

한편, 본 발명의 표고버섯으로부터 베타-글루칸을 추출하는 방법에 있어서,상기 표고버섯은, 일 예로 건(乾) 표고버섯인 것일 수 있다. On the other hand, in the method for extracting beta-glucan from the shiitake mushroom of the present invention, the shiitake may be, for example, dried shiitake.

한편, 본 발명의 표고버섯으로부터 베타-글루칸을 추출하는 방법에 있어서, 상기 베타-글루칸은, 바람직하게 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸일 수 있다. 본 발명에 의할 경우, 베타-글루칸 중 특히 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸의 추출 수율이 높게 나타났다.
Meanwhile, in the method of extracting beta-glucan from the shiitake mushroom of the present invention, the beta-glucan may be preferably beta-glucan having beta-1, 6-branch beta-1,3 bonds. According to the present invention, the extraction yield of beta-glucan having β-1, 6-branching β-1, 6-branching was particularly high among the β-glucans.

본 발명의 열수 추출 및 초고압 추출의 순차적 병행 추출방법에 의할 경우, 단순 열수 추출 또는 단순 초고압 추출에 비해 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸의 추출 수율이 약 20~30% 정도 향상됨을 확인할 수 있었다.
According to the sequential simultaneous extraction method of hot water extraction and ultra-high pressure extraction according to the present invention, the extraction yield of beta-glucan having β-1,6-branching β-1,3 bond is about 20 ~ 30%.

이하, 본 발명의 내용을 하기 실시예 및 실험예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다.
Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[[ 실시예Example : 건(乾) 표고버섯으로부터 베타-: From dry shiitake mushrooms to beta - 글루칸Glucan 함유 추출물의 제조] Preparation of containing extracts]

본 실시예에서는 하기 표 1 및 표 2에 기재된 조건에 의해 건(乾) 표고버섯으로부터 베타-글루칸 함유 추출물을 제조하고자 하였다. 표 1에서는 건(乾) 표고버섯을 칼로 컷팅하여 3 mm의 크기로 절편한 샘플(슬라이스) 350 g을 사용하였고, 표 2에서는 건(乾) 표고버섯을 100 메쉬 정도로 분말화한 샘플(분말) 350 g을 사용하였다. In this Example, the extracts containing beta-glucan were prepared from dried shiitake mushrooms under the conditions described in Tables 1 and 2 below. In Table 1, 350 g of a sample (slice) cut into a size of 3 mm by cutting a dried shiitake mushroom with a knife was used. In Table 2, a sample (powder) obtained by pulverizing dried shiitake mushroom to about 100 mesh was used. 350 g was used.

'가압 열수 추출'은 가압 열수 추출조(DAIHAN)에 슬라이스 또는 분말화한 건(乾) 표고버섯 샘플을 넣고, 하기 표 1 및 2에 기재된 만큼의 용매(물)를 넣은 후, 하기 표 1 및 2에 기재된 가압 열수 조건을 사용하여 추출한 것이다. In the "pressurized hot water extraction", a sliced or powdered dried shiitake mushroom sample was placed in a pressurized hot water extraction tank (DAIHAN), and the solvent (water) as shown in Tables 1 and 2 was added, 2 < / RTI >

'초고압 추출'은 초고압 추출조(TOYO KOATSU CO., LTD)에 슬라이스 또는 분말화한 건(乾) 표고버섯 샘플을 넣고, 하기 표 1 및 2에 기재된 만큼의 용매(물)를 넣은 후, 하기 표 1 및 2에 기재된 초고압 추출 조건을 사용하여 추출한 것이다.'Extra-high pressure extraction' is a method in which a slice or powdered shiitake mushroom sample is put into an ultra-high pressure extraction tank (TOYO KOATSU CO., LTD.), And the solvent (water) And extracted using the ultra-high pressure extraction conditions described in Tables 1 and 2.

'가압 열수 추출 → 초고압 추출'은 추출조에 슬라이스 또는 분말화한 건(乾) 표고버섯 샘플을 넣은 후, 하기 표 1 및 2에 기재된 만큼의 용매(물)를 넣고, 하기 표 1 및 2에 기재된 가압 열수 조건을 사용하여 추출한 후, 추출물을 그대로 초고압 추출조에 옮겨 담고, 하기 표 1 및 2에 기재된 초고압 추출 조건을 사용하여 추출한 것이다.In the "pressurized hot water extraction → ultra-high pressure extraction", a slice or powdered dried shiitake mushroom sample was placed in an extraction tank, and the solvent (water) as shown in Tables 1 and 2 below was added, Pressure hydrothermal condition, and then the extract was directly transferred to the ultra-high pressure extraction tank and extracted using the ultra-high pressure extraction conditions described in Tables 1 and 2 below.

'초고압 추출 → 가압 열수 추출'은 초고압 추출조에 슬라이스 또는 분말화한 건 표고버섯 샘플을 넣은 후, 하기 표 1 및 2에 기재된 만큼의 용매(물)를 넣고, 하기 표 1 및 2에 기재된 초고압 추출 조건을 사용하여 추출한 후, 추출물을 그대로 열수 가압 추출조에 옮겨 담고, 하기 표 1 및 2에 기재된 가압 열수 조건을 사용하여 추출한 것이다. In the "ultra-high pressure extraction → pressurized hot water extraction", a slice or powdered dried shiitake mushroom sample was put in an ultra-high pressure extraction tank, and the solvent (water) as shown in Tables 1 and 2 below was added, And then the extract was directly transferred into a hot water pressure extracting tank and extracted using the pressurized hot water condition described in Tables 1 and 2 below.

슬라이스한 건(乾) 표고버섯 사용Use a slice of shiitake mushroom 실시예Example 추출법Extraction method 추출 조건Extraction condition 용매비Solvent ratio 추출온도Extraction temperature 추출시간Extraction time 압력pressure 동결freezing
건조dry
1One 가압 열수 추출Pressurized hot water extraction 1:201:20 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2

48시간


48 hours
22 초고압 추출Ultra high pressure extraction 1:201:20 60℃60 ° C 3시간3 hours 100MPa100 MPa 33 가압 열수 추출
→ 초고압 추출
Pressurized hot water extraction
→ Extra-high pressure extraction
1:201:20 열수Heat number 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2
초고압Ultrahigh pressure 60℃60 ° C 3시간3 hours 100MPa100 MPa 44 초고압 추출
→ 가압 열수 추출
Ultra high pressure extraction
→ Pressurized hot water extraction
1:201:20 초고압Ultrahigh pressure 60℃60 ° C 3시간3 hours 100MPa100 MPa
열수Heat number 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2

분말화한 건(乾) 표고버섯 사용 Powdered (dried) shiitake 실시예Example 추출법Extraction method 추출 조건Extraction condition 용매비Solvent ratio 추출온도Extraction temperature 추출시간Extraction time 압력pressure 동결freezing
건조dry
55 가압 열수 추출Pressurized hot water extraction 1:201:20 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2

48시간


48 hours
66 초고압 추출Ultra high pressure extraction 1:201:20 60℃60 ° C 3시간3 hours 100MPa100 MPa 77 가압 열수 추출
→ 초고압 추출
Pressurized hot water extraction
→ Extra-high pressure extraction
1:201:20 열수Heat number 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2
초고압Ultrahigh pressure 60℃60 ° C 3시간3 hours 100MPa100 MPa 88 초고압 추출
→ 가압 열수 추출
Ultra high pressure extraction
→ Pressurized hot water extraction
1:201:20 초고압Ultrahigh pressure 60℃60 ° C 3시간3 hours 100MPa100 MPa
열수Heat number 110℃110 ° C 3시간3 hours 1.25kgf/㎠1.25 kgf / cm 2

상기의 방법대로 추출한 실시예 1~8의 추출 분말 (동결건조 분말)에 대해, 하기의 실험을 수행하여 베타-글루칸의 함량을 측정하였다.
For the extracted powders (lyophilized powders) obtained in Examples 1 to 8, the content of beta-glucan was measured by the following experiment.

[[ 실험예Experimental Example 1: 상기  1: 실시예Example 샘플의 베타- The beta - 글루칸Glucan 함량 측정] Content measurement]

본 실험예에서는 상기에서 추출한 실시예 1~8의 추출물에 대해 베타-글루칸 함량 및 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸 함량을 측정하였다. In this Experimental Example, the content of beta-glucan and the content of beta-glucan having beta-1, 6 branching beta-1,3 bonds were measured for the extracts of Examples 1 to 8 extracted above.

(1) 총 베타-글루칸 함량 측정  (1) Total beta-glucan content measurement

건강기능식품공전에 기재된 베타-글루칸 측정 시험방법을 사용하여, 총 베타-글루칸 함량을 측정하였다. 측정된 총 베타-글루칸 함량을 표 3에 나타내었고, 추출물 중량 중 총 베타-글루칸의 함량을 %로 표시하였다. The total beta-glucan content was measured using the beta-glucan measurement test method described in the Health Functional Food Cycle. The measured total beta-glucan content is shown in Table 3, and the content of total beta-glucan in the extract weight was expressed in%.

(2) β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸 함량 측정 (2) Measurement of beta-glucan content having beta-1, 6-branch beta-1,3 bonds

"Mushroom and yeast beta-glucan assay procedure kit (Megazyme)"를 사용하여, 해당 키트에 기재된 프로토콜대로 실험을 수행하여, β-1,3 1,6 결합을 갖는 베타-글루칸 함량을 측정하였다.Using the "Mushroom and yeast beta-glucan assay procedure kit (Megazyme)", an experiment was conducted according to the protocol described in the kit to determine the content of beta-glucan having β-1,3 1,6 linkages.

베타-글루칸 함량 측정 결과Results of beta-glucan content measurement 실시예
Example
베타-글루칸 함량(%)Beta-glucan content (%)
총 베타-글루칸 함량Total beta-glucan content β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸beta-glucan with beta-1, 6-branch beta-1,3 linkages 1One 9.649.64 9.179.17 22 13.8613.86 9.739.73 33 13.5913.59 13.1313.13 44 11.8511.85 10.9710.97 55 11.8911.89 11.0611.06 66 11.8211.82 10.8010.80 77 11.6111.61 11.0711.07 88 13.0713.07 11.3611.36

실험 결과, 상기 표 3에서 살펴본 바와 같이, 실시예 3 샘플(슬라이스 건(乾) 표고버섯에 대해 '열수 가압 추출 → 초고압 추출'을 수행한 샘플)에서, 표고버섯에 주되게 함유되어 있는 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸의 함량이 다른 실시예 샘플들에 비해 20~30% 정도 높게 나타났다. As a result of the experiment, as shown in Table 3, in the sample of Example 3 (sample obtained by performing hydrothermal pressurization → ultra-high pressure extraction on sliced dried shiitake), the β- The content of beta-glucan having 1,6-branched beta-1,3 linkages was 20 to 30% higher than those of other example samples.

Claims (4)

슬라이스된 건(乾) 표고버섯에 100~120℃의 열수를 표고버섯 중량의 15~25배만큼 첨가하고 1.25 kgf/cm2의 압력을 가하여 2~4시간 동안 열수 추출하는 단계(a);
상기 단계(a) 후, 온도를 50~70℃로 조정하고, 90~110 MPa의 초고압을 가하여 2~4시간 동안 초고압 추출하는 단계(b);를 포함하여 표고버섯으로부터 베타-글루칸을 추출하며,
총 베타-글루칸 추출함량에서 β-1,6 분지 β-1,3 결합을 갖는 베타-글루칸의 함량을 증진시키는 것을 특징으로 하는 표고버섯으로부터 베타-글루칸을 추출하는 방법.
(A) adding hot water at a temperature of 100 to 120 ° C to sliced dried shiitake at a rate of 15 to 25 times the weight of shiitake and applying a pressure of 1.25 kgf / cm 2 for 2 to 4 hours;
(B) extracting beta-glucan from the shiitake mushroom by adjusting the temperature to 50 to 70 ° C and extracting the extract at an ultra-high pressure of 90 to 110 MPa for 2 to 4 hours; ,
The method of extracting beta-glucan from a mushroom characterized in that the content of beta-glucan having beta-1, 6-branch beta-1,3 bonds is enhanced in the total beta-glucan extract content.
제1항에 있어서,
상기 표고버섯으로부터 베타-글루칸을 추출하는 방법은,
단계(b) 후, 동결건조하는 단계(c);를 추가로 포함하는 것을 특징으로 하는 표고버섯으로부터 베타-글루칸을 추출하는 방법.
The method according to claim 1,
The method for extracting beta-glucan from the mushroom comprises:
(C) a step of lyophilization after step (b). ≪ Desc / Clms Page number 20 >
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