KR101538024B1 - Multi nozzle type scaffold manufacturing device and method for using the same - Google Patents

Abstract

The present invention relates to an apparatus for manufacturing a multi-nozzle type cell culture support and a method for manufacturing a cell culture support using the same, wherein a plurality of injection means including a spray nozzle and a solution storage portion are formed, Not only is it easy to contact the distance sensor part formed at a certain distance from the collecting part and measures how far the end of each injection nozzle is actually spaced from the distance sensor part, The present invention relates to a device for manufacturing a cell culture support and an apparatus for manufacturing a cell culture support using the same.

Description

[0001] The present invention relates to a device for manufacturing a multi-nozzle type cell culture support and a method for manufacturing a cell culture support using the same,

The present invention relates to an apparatus for manufacturing a multi-nozzle type cell culture support and a method for manufacturing a cell culture support using the same, wherein a plurality of injection means including a spray nozzle and a solution storage portion are formed, Not only is it easy to contact the distance sensor part formed at a certain distance from the collecting part and measures how far the end of each injection nozzle is actually spaced from the distance sensor part, The present invention relates to a device for manufacturing a cell culture support and an apparatus for manufacturing a cell culture support using the same.

Tissue engineering is a technique in which a tissue is taken from the body of a patient and the cells are separated from the tissue. The separated cells are then expanded by the necessary amount by culturing and planted in a porous biodegradable polymer scaffold. And then implanting the hybrid cell / polymer construct back into the human body.

After transplantation, most tissues and organs are fed with oxygen and nutrients by diffusion of body fluids until new blood vessels are formed. When blood vessels enter the body and blood is supplied, cells multiply and differentiate into new tissues and organs And the application of the polymer scaffold to dissolve and disappear.

Therefore, it is important to prepare a biodegradable polymer cell culture support similar to a biomedical tissue for such tissue engineering studies.

The main requirement of the support material used for the regeneration of human tissue is that it should have mechanical strength sufficient to allow the tissue cell to adhere to the surface of the material to form a tissue having a three-dimensional structure, , It should also act as an intermediate barrier between the transplanted and host cells, which requires a non-toxic biocompatibility that does not result in blood clotting or inflammation after transplantation.

In addition, when the transplanted cells function as a new body tissue, they must have biodegradability that can be completely decomposed and disappeared in a desired period of time.

Currently widely used biodegradable polymers such as PGA, PLA and PLGA, biodegradable polymers that can be used in the human body have been used as a porous polymer cell culture support material for regenerating the body tissue.

The structure of the cell culture support for biomedical engineering requires a high porosity for a large surface area enabling high density of cell adhesion, and it is required that the formation of blood vessels and the transfer of nutrients such as growth factors and hormones after in vivo implantation It is necessary to have a large pore and an open cell structure interconnected with the pore space and to control the porosity and pore shape according to the characteristics of the tissue to be cultured.

Recently, several researchers have attempted various methods for making a polymer into a porous structure, one of which is to physically or mechanically form different cell culture supports in a single layer alternately or in a multi-layer structure, So that they have different characteristics.

In order to simultaneously form cell culture supporters having different characteristics, a solution storage tank 11, a spray tube 12, and a spray tube 12 are provided in a domestic registered patent No. 1153728 (registered on May 31, 2012, There is disclosed a cell culture support comprising a plurality of injection means 10 composed of an injection nozzle 13 and a transfer device 14, a collecting portion 15 and a controller 16, same.

When a cell culture support is manufactured using the cell culture support manufacturing apparatus as shown in FIG. 1, controlling the position where the biocompatible material is injected for each of the injection nozzles greatly affects the quality of the cell culture support.

In particular, the injection nozzle 13 may have a long nozzle as needed, and a short nozzle may be used. In the cell culture support production, the initial position in the z-axis of the injection nozzle, (15) is very important, it is necessary to reset the initial position every time the nozzle is changed.

In addition, the injection nozzle may be installed in a skewed manner during the installation process, so that initial height adjustment is necessarily required.

Conventionally, in order to adjust the initial height of the injection nozzle, the operator manually adjusts the distance between the nozzle and the floor while checking the distance between the nozzle and the floor. This increases the working time and reduces efficiency and accuracy.

In addition, the injection means of the apparatus for manufacturing a cell culture support as shown in FIG. 1 is formed such that the injection nozzle is fixed only by a thread catching portion which is inserted and fixed in the horizontal direction when the injection nozzle is assembled so that the end of the injection nozzle is shaken or skewed, Thereby reducing the accuracy of the measurement.

Domestic registered patent No. 1153728 (registered on May 31, 2012, name: Cell culture support support manufacturing apparatus and manufacturing method)

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to provide a multi-cell culture support comprising a plurality of spraying means composed of an injection nozzle and a solution storage portion, And it is also possible to measure how far the end of each injection nozzle is actually spaced apart from the distance sensor portion by contacting the distance sensor portion formed with a certain distance from the collecting portion, And a method of manufacturing a cell culture support using the same, which can improve the efficiency of manufacturing work and the manufacturing precision by automatically aligning the z-axis initial position of the injection nozzle.

It is also an object of the present invention to provide a method and an apparatus for measuring an accuracy of an injection position by measuring the distance of each of the plurality of injection nozzles at a point of time when a CCD camera is in contact with a reference point, And a method of manufacturing a cell culture support using the same.

It is another object of the present invention to provide a fixing device capable of pressing and fixing an upper end of a solution storage part to prevent the injection nozzle from being shaken or skewed because there is no pressing part due to its own weight during assembly of the injection device, And to provide a device for manufacturing a cell culture support.

The apparatus for manufacturing a multi-nozzle type cell culture support according to the present invention comprises a solution storage part for storing a biocompatible material, an injection nozzle for spraying a solution supplied from the solution storage part, a heating part for heating the solution stored in the solution storage part A plurality of injection means formed to include a portion; A planar collecting unit provided below the injecting unit to collect a solution injected through the injecting nozzle of the injecting unit and having a temperature adjusting unit therein; An injection position regulating unit formed to include an x-axis transfer unit and a y-axis transfer unit for transferring a plurality of the injection units at the same time in the x-axis and the y-axis, and a z-axis transfer unit for transferring the plurality of the injection units in the z- And a distance sensor unit spaced apart from the collecting unit by a predetermined distance and measuring a distance from the injection unit in the z-axis direction until the end of the injection nozzle contacts the nozzle unit. A control unit for calculating an absolute coordinate of each of the injection nozzles through a vertical distance between the distance sensor unit and the injection nozzle end measured by the distance sensor unit, and controlling the injection position and the collection position; As shown in FIG.

In addition, the multi-nozzle type cell culture support body is spaced apart from the distance sensor unit by a predetermined distance, and the ends of each of the injection nozzles are sequentially positioned at a reference point for measuring distances in x and y directions between adjacent injection nozzles And a position alignment means which is a CCD camera to be focused when the CCD camera is in focus.

The control unit may measure the x and y axis travel distances of the respective injection nozzles when the end of the injection nozzle is positioned at the reference point and the CCD camera is focused, And the position of the injection nozzle can be adjusted through the x-axis transfer part and the y-axis transfer part.

Wherein the injection unit is formed with a hollow portion in which the injection nozzle is separately connected to a lower portion of the solution storage portion and into which the solution storage portion is inserted, and a first support portion and a second support portion, And a fixing unit connected to the z-axis conveying unit through a support formed to include the solution reservoir and the spray nozzle.

The fixing means is hinged to both ends of the third supporting base of the supporting base protruding in the y-axis direction on the surface connected to the z-axis conveying unit so as to be positioned on both sides in the x-axis direction of the heating unit, A ' ' shaped < / RTI > A first fixing part formed on a surface of the solution storage part facing the upper surface of the solution storage part in a state where the rotation link rotates and is parallel to the z axis and presses and fixes the solution storage part in the z axis direction; A second fixing part formed on the first support and pressing and fixing the solution storage part in the y axis direction; As shown in FIG.

In the method for manufacturing a cell culture support using a multi-nozzle type cell culture support production apparatus of the present invention, it is preferable that: a) the injection means is transported in the x- and y- So as to face the distance sensor unit vertically; b) the injection nozzle is lowered to contact the distance sensor unit; c) calculating a z-axis absolute coordinate of the injection nozzle by measuring a distance by which the injection nozzle contacts the distance sensor unit by the control unit; d) performing steps a) to c) for the remaining injection means to calculate the absolute coordinates of each of the injection means; e) automatically aligning the initial position so that any one of the spraying nozzles of the spraying means is located at a position where the biocompatible material of the collecting portion is to be sprayed by transferring the spraying means in the x-axis, y-axis and z-axis; . ≪ / RTI >

In the method of manufacturing a multi-nozzle type cell culture support, the separation distance between the collecting part and the injection nozzle may be set differently according to the diameter of the injection nozzle in step e), so that the initial position of the injection nozzle may be aligned .

In addition, the method for manufacturing a cell culture support may further comprise the steps of: d) between steps d) and e); f) moving the injection means in the x-, y-, and z- A focusing step for focusing the light beam on the light source; g) a transfer distance measurement step in which the transfer distance of each of the injection nozzles 120 is measured by the control unit 500 after the CCD camera is focused; h) calculating a distance between neighboring injection nozzles by measuring the distance by which the injection nozzle contacts the reference point by the control unit; Can be performed.

In addition, in the method for manufacturing a cell culture support, the steps f) to h) may be performed at the initial setting of the cell culture support manufacturing apparatus.

In addition, in the method for manufacturing a cell culture support, a cell culture support having a multi-layer structure in which different biocompatible materials are injected for each layer can be produced.

In addition, in the cell culture support manufacturing method, a cell culture support in which different biocompatible materials are alternately injected into the same layer can be produced.

In addition, when a plurality of cell culture supports are simultaneously produced through a plurality of the injection means, each cell culture support may be made of the same biocompatible material or may be made of the same biocompatible material.

The multi-nozzle type cell culture support and method for manufacturing a cell culture support using the same according to the present invention include a plurality of injection nozzles and contact with a distance sensor portion formed at a distance from the collecting portion, An actual distance from the distance sensor unit is measured and then the initial position of the z-axis of the injection nozzle is automatically aligned using the measured distance, thereby improving the manufacturing efficiency and manufacturing accuracy.

That is, according to the present invention, when a cell culture support manufacturing apparatus including a plurality of injection nozzles is used to form cell culture supports alternately with different materials in a multi-layer structure or a single layer, each of the injection nozzles Eliminating the trouble of manually adjusting the height of the injection nozzle by manually checking the position of the nozzle in order to align the nozzle at a predetermined position and precisely aligning the injection nozzle precisely to improve accuracy and precision of the process have.

In addition, the present invention measures the accuracy of the injection position and the manufacturing speed of the injection nozzle by calculating the x-axis and y-axis positions of the injection nozzle by measuring the distance of each of the plurality of injection nozzles when the CCD camera comes into contact with the reference point, The efficiency of the work can be improved.

In addition, the apparatus for manufacturing a multi-nozzle type cell culture support according to the present invention is characterized in that there is no pressing portion due to its own weight during assembly of the injection means, so that the injection nozzle is prevented from being shaken or skewed, There is an effect that the work precision can be improved.

Therefore, according to the present invention, it is possible to solve the problem of machining inaccuracies or impossibility which can be caused by shaking the injection nozzle or deviating from the correct position during the operation of mounting the injection nozzle in a process of assembling the injection means, Can be originally prevented.

In other words, the apparatus for manufacturing a multi-nozzle type cell culture support according to the present invention can precisely align the injection nozzle with no deformation at an accurate position, thereby realizing the precise cell culture support to be manufactured, and the quality of the prepared cell culture support There is a big effect that can greatly improve.

In addition, the apparatus for manufacturing a multi-nozzle type cell culture support of the present invention can form a complex cell culture support made of various materials through a plurality of injection means, Compared to the support, the properties can be changed in one cell culture support according to the characteristics of different materials, and the degradation rate can be changed, so that applicable fields can be further diversified.

1 is a conventional apparatus for manufacturing a cell culture support.
FIG. 2 is a perspective view illustrating a device for manufacturing a multi-nozzle type cell culture support according to an embodiment of the present invention. FIG.
FIG. 3 is a front view illustrating a state in which a spray nozzle contacts a distance sensor unit in an apparatus for manufacturing a multi-nozzle type cell culture support according to an embodiment of the present invention. FIG.
FIG. 4 and FIG. 5 are a perspective view and an exploded perspective view showing the injection means in the cell culture support manufacturing apparatus of FIG. 2;
6 is a perspective view of a multi-nozzle type cell culture support manufacturing apparatus according to another embodiment of the present invention.
FIG. 7 is a front view showing a state in which an injection nozzle is disposed on a position alignment means in the cell culture support manufacturing apparatus of FIG. 6 to measure x-axis and y-axis coordinates;
8 is a view showing reference points on a CCD camera in the cell culture support manufacturing apparatus of FIG. 6;
9 to 11 are perspective views showing various embodiments of a cell culture support that can be manufactured using the multi-nozzle type cell culture support manufacturing apparatus of the present invention.

Hereinafter, an apparatus for manufacturing a multi-nozzle type cell culture support and a method of manufacturing a cell culture support using the same according to the present invention will be described in detail with reference to the accompanying drawings.

Example 1

In Embodiment 1, a multi-nozzle type cell culture support manufacturing apparatus 1 according to an embodiment of the present invention will be described with reference to FIGS. 2 and 3. FIG.

2, the apparatus 1 for manufacturing a cell culture support according to the present invention mainly includes a spraying unit 100, a collecting unit 200, a spraying position adjusting unit 300, a distance sensor unit 400, (500).

Although FIG. 2 shows an example in which three injection means 100 are formed, the number of the injection means 100 may be two, four, or more.

For convenience of explanation, the following description will be made on the assumption that the number of the injecting means 100 is three, and it is referred to as a first injecting means 100, a second injecting means 100, and a third injecting means 100 do.

The injection means 100 includes a solution storage portion 110 for storing the biocompatible material, a spray nozzle 120 for spraying the solution supplied from the solution storage portion 110, And a heating unit 130 for controlling the temperature of the solution.

As described above, when the number of the injection means 100 is three, the first injection means 100, the second injection means 100, and the third injection means 100, and the first injection means 100 Includes a first solution storage part 111, a first injection nozzle 121 and a first heating part 131 and the second injection device 100 includes a second solution storage part 112, The third spraying unit 123 and the third heating unit 133. The third spraying unit 123 includes a third spraying nozzle 123 and a third heating unit 133, . ≪ / RTI >

At this time, biocompatible materials having different characteristics can be stored in the first solution storage part 111, the second solution storage part 112, and the third solution storage part 113, Biocompatible polymer, biomaterial or bioprotein. Specific examples thereof include polycaprolactone (PCL), polylactide (PLA), polyglycolide (PGA), polylactic glycolide (PLGA), polydioxane (PDO), polylactic acid (PLLA), and the like. Of course, any other biomaterial that can be melted and printed by heating can be changed.

The injection nozzle 120 injects the solution supplied from the solution storage part 110 and is supplied to the first injection nozzle 121, the second injection nozzle 122 and the third injection nozzle 123, May have different diameters.

The injection nozzle 120 may be separately connected to the lower part of the solution storage part 110 and may be detachable.

Accordingly, the multi-nozzle type cell culture support manufacturing apparatus 1 of the present invention includes a plurality of injection means 100, so that a composite cell culture support can be manufactured from different materials.

The heating part 130 may be in the form of a heat wire surrounding the solution storage part 110 and may include a hollow part 134 inside to hollow the solution storage part 110 .

The collecting unit 200 is provided at a lower portion of the injecting unit 100 to collect the solution injected through the injecting nozzle 120 of the injecting unit 100 in the form of a flat plate, Feeds the injection means 100 in the x-, y-, and z-axis directions to adjust the injection position.

The collecting unit 200 may further include a temperature adjusting unit 210 in the form of a hot wire to adjust the temperature from room temperature to about 100 ° C depending on the material and the environment. At this time, the temperature regulating means 210 can be changed in any form other than the hot wire.

The injection position regulating unit 300 includes an x-axis feed unit 310 for feeding the first injecting unit 100, the second injecting unit 100 and the third injecting unit 100 at one time in the x and y axes, a y-axis transferring unit 320 and a z-axis transferring unit 330 for transferring the first injecting unit 100, the second injecting unit 100 and the third injecting unit 100 along the z axis, .

That is, the injection position regulating unit 300 controls the first injecting unit 100, the second injecting unit 100, and the third injecting unit 100 simultaneously in the x-axis and the y-axis, while the z- Respectively.

Of course, the first injecting means 100, the second injecting means 100 and the third injecting means 100 can be controlled individually also in the x-axis and the y-axis as well as in the z-axis, Control over x and y axes does not have a significant effect on the process of injecting a biocompatible material even if they are controlled at the same time, You can help.

The distance sensor unit 400 is spaced apart from the collecting unit 200 by a predetermined distance and the distance from the injection unit 100 to the end of the injection nozzle 120 in the z- It plays a role of measuring.

The control unit 500 calculates the absolute coordinates of each of the injection nozzles 120 through a vertical distance between the distance sensor unit 400 and the end of the injection nozzle 120 measured by the distance sensor unit 400 , The injection position and the collecting position.

That is, the distance sensor unit 400 detects a distance from a moment when any one of the first injecting unit 100, the second injecting unit 100, and the third injecting unit 100 is positioned at a vertical position in the z- The second injection nozzle 122, and the third injection nozzle 123 can be measured by moving the first injection nozzle 121, the second injection nozzle 122, and the third injection nozzle 123, The control unit 500 can calculate the absolute coordinates of each of the spray nozzles 120 to accurately align the spray nozzles 120 at the initial position for the cell culture support S manufacturing process And the automatic control by the control unit 500 is enabled.

For example, if the upper end of the first injection nozzle 121, the second injection nozzle 122 and the third injection nozzle 123 are located at the same position and the distance of the collecting part 200 from the upper end is 50 mm Is 2 mm from the collecting part 200 when the length of the first injection nozzle 121 is 3 mm, the second injection nozzle 122 is 2 mm and the third injection nozzle 123 is 1 mm The first injection nozzle 121 may be lowered by 45 mm in the z-axis direction, the second injection nozzle 122 may be 46 mm, the third injection nozzle 123 ) Is lowered by 47 mm.

Accordingly, the present invention provides a method for forming a cell culture support (S) by alternately forming a cell culture support (S) in a multi-layer structure or a single layer with different materials using a cell culture support manufacturing apparatus (1) including a plurality of injection nozzles It is possible to eliminate the trouble of manually adjusting the height of the injection nozzle 120 while manually checking the positions of the respective injection nozzles 120 at a predetermined position, The accuracy and precision of the process can be greatly improved.

Example 2

In Embodiment 2, referring to Figures 4 and 5, the injection means 100 in the apparatus for manufacturing a multi-nozzle type cell culture support 1 of the present invention will be described.

3, the injection unit 100 is connected to the spray nozzle 120 separately below the solution storage unit 110, and the solution storage unit 110 And a support 150 formed with a first support 151 and a second support 152 for supporting the upper and lower surfaces of the heating unit 130 And a fixing unit 160 connected to the z-axis transfer unit 330 and fixing the solution storage unit 110 and the injection nozzle 120 to the support 150.

In other words, the solution storage part 110 is inserted into the hollow part 134 of the heating part 130, and the injection nozzle 120 is detachably coupled to the lower end of the solution storage part 110. The support 150 may include a first support 151 directly connected to the z-axis transfer part 330 or connected to the z-axis transfer part 330 through a separate bracket and contacting the upper surface of the heating part 130, A second support 152 which is in contact with and supports the lower side of the heating unit 130 and a second support 152 which is in contact with the z-axis transport unit 330 to be positioned on both sides of the heating unit 130 in the x- And a third support 153 protruding from the second support 153.

Particularly, in the apparatus for manufacturing a multi-nozzle type cell culture support 1 of the present invention, the injection means 100 is arranged such that the injection nozzle 120 and the solution storage portion 110 are connected to the heating portion 130 and the support 150 The fixing unit 160 may further include a fixing unit 160 that is stably fixed so as not to be shaken or to change the position of the injection nozzle 120.

The fixing means 160 includes a rotation link 161 having a U-shaped shape that is hinged to the third support 153 and hinged to both ends of the third support 153, A first fixing part 162 formed on the surface of the solution storage part 110 facing the upper surface of the solution storage part 110 in parallel with the z axis and for pressing and fixing the solution storage part 110 in the z- And a second fixing part 163 formed on the first support 151 and pressing and fixing the solution storage part 110 in the y-axis direction.

4 and 5, the assembling process of the injecting unit 100 will be briefly described. First, the heating unit 130 (upper and lower surfaces) fixed to the first and second supports 151 and 152 The solution storage part 110 and the injection nozzle 120 are inserted into the hollow part 134 of the nozzle body 110. [

The rotation link 161 hinged to both ends of the third support 153 is rotated to be positioned at the upper end of the solution storage unit 110 and then the first fixing unit 162 is moved downward And presses the solution storage part 110 and the injection nozzle 120 in the gravity direction.

Next, the second fixing portion 163 is rotated to press the solution storage portion 110 in the y-axis direction so that the solution storage portion 110 can be stably fixed in the heating portion 130 .

In this case, the first fixing part 162 and the second fixing part 163 are bolts formed with flat bolt heads at both ends thereof, and may be rotated and inserted in a clockwise direction, and may be variously modified. Is possible.

Accordingly, the apparatus for manufacturing a multi-nozzle type cell culture support 1 according to the present invention is characterized in that there is no pressing portion due to its own weight when assembling the injection means 100, and therefore, in order to prevent the injection nozzle 120 from being shaken or skewed, The fixing unit 160 can be fixed by pressing the upper end of the part 110, thereby improving the working accuracy.

That is, according to the present invention, when the injection nozzle 120 is installed in a distorted state during the assembly of the injection means 100, or when the cell culture support S is manufactured, the injection nozzle 120 is shaken or deviated The machining inaccuracies or inconveniences that can occur can be prevented at the source.

Example 3

In Embodiment 3, a multi-nozzle type cell culture support manufacturing apparatus including a positioning means 600 will be described with reference to FIGS. 6 and 8. FIG.

The apparatus for manufacturing a multi-nozzle type cell culture support of the present invention may further include a positioning unit 600 as shown in FIG. 6, wherein the positioning unit 600 is spaced apart from the distance sensor unit by a predetermined distance And the end of each of the injection nozzles 120 is sequentially positioned at a reference point 610 for measuring the distance between the adjacent injection nozzles 120 in the x and y axes.

At this time, the reference point 610 may be a crossing point of a cross shape as shown in FIG.

The control unit measures the x and y axis transfer distances of the injection nozzles 120 when the end of the injection nozzle 120 is positioned at the reference point 610 and the CCD camera is focused, The distance between the adjacent injection nozzles 120 is calculated through the distance of each of the injection nozzles 120, and the position of the injection nozzle 120 is adjusted through the x-axis transfer part and the y-axis transfer part.

As described above, the apparatus for manufacturing a cell culture support of the present invention includes a plurality of injection nozzles 120, and the injection nozzles 120 are sequentially transferred to be positioned above the collecting unit 200, The cell culture support S having different characteristics can be formed into a multi-layered structure.

At this time, in order to form a cell culture support S having a multi-layered structure, the cell culture support manufacturing apparatus of the present invention is adjusted so that the injection position of the injection nozzle 120 is constant for each layer, Through the control unit to sequentially position the end of each of the injection nozzles 120 at a reference point 610 for measuring the intervals d1 and d2 between adjacent injection nozzles 120 through the control unit The distances d1 and d2 between the injection nozzles 120 can be calculated through the difference in the transfer distance of each of the injection nozzles 120 after measuring the transfer distance from the reference point 610 to the reference point 610. [

Here, d1 means an interval in the x-axis direction between adjacent injection nozzles 120, and d2 means an interval in the y-axis direction between adjacent injection nozzles 120. [

The control unit 500 calculates the distance between the injection nozzles 120 and then dispenses the droplets onto the collecting unit 200 by the first injection nozzle 120. Then, the y-axis transfer unit is controlled to transfer the spraying unit 100 by a distance between the first spraying nozzle 120 and the second spraying nozzle 120 so that the second nozzle is sprayed.

In other words, in the cell culture support manufacturing apparatus of the present invention, the control unit 500 sequentially positions the respective injection nozzles 120 at a predetermined position of the collecting unit 200 to spray the solution, A cell culture support S having different characteristics is formed on different layers so that the solutions stored in the solution storage part 110 are injected alternately through the injection nozzle 120, (S) can be formed.

The position alignment unit 600 not only allows the end of the injection nozzle 120 to be positioned at the reference point 610 but also transmits the time point of the reference point 610 to the control unit 500, So that the distance to which the injection nozzle 120 is transferred from the reference point 610 can be calculated.

In this case, the position alignment unit 600 may be a CCD camera that is focused when the end of the injection nozzle 120 is positioned at the reference point 610 and can transmit certain data to the control unit.

Referring to FIG. 6, the process for producing a cell culture support S having a multilayer structure will be described. In the cell culture support production apparatus of the present invention, the intervals d1 and d2 between the injection nozzles 120 are measured, Th layer is completed, the control unit 500 causes the x-axis transport unit and the y-axis transport unit to be transported by the distance d1 and d2 between the injection nozzles 120, so that the second layer is ejected.

That is, when the end of the injection nozzle 120 is positioned at the reference point 610 and the CCD camera is focused, the control unit measures the distance of each of the injection nozzles 120, D2 of the adjacent injection nozzles 120 through the transfer distance of the collecting unit 200 so that the injection nozzle 120 is positioned at a predetermined position of the collecting unit 200, So that the conveying portion is adjusted.

Example 4

In Embodiment 4, a method of manufacturing a cell culture support using the apparatus for manufacturing a multi-nozzle type cell culture support 1 of the present invention will be described with reference to Figs. 9 to 11. Fig.

The method for manufacturing a cell culture support according to the present invention comprises the steps of: a) transferring the injection means 100 in the x-axis and y-axis directions so that the end of any one of the plurality of injection means 100, (400) in a vertical direction; b) the injection nozzle 120 is lowered to contact the distance sensor unit 400; c) measuring a distance by which the injection nozzle 120 contacts the distance sensor unit 400 by the controller 500 and calculating the absolute coordinates of the injection nozzle 120; d) performing steps a) to c) for the remaining injection means (100) to calculate the absolute coordinates of each of the injection means (100); e) the injection means 100 is moved in the x-axis, the y-axis and the z-axis so that any one of the injection nozzles 120 of the injection means 100 Automatically aligning the initial position so that the initial position is located; .

A method for manufacturing the cell culture support (S) shown in Fig. 9 using the apparatus for manufacturing a multi-nozzle type cell culture support (1) of the present invention will be described.

First, the injection means 100 is transferred on the x-axis and the y-axis through the x-axis transferring portion 310 and the y-axis transferring portion 320 so that the first injection nozzle 121 of the first injection means 100 And the first injection nozzle 121 is brought into contact with the distance sensor unit 400 through the z-axis transporting unit 330. The distance sensor unit 400 may be disposed at a predetermined position.

At this time, the controller 500 measures the distance until the first injection nozzle 121 contacts the distance sensor unit 400, and calculates the absolute coordinates of the first injection nozzle 121 .

Next, after the end of the second injection nozzle 122 of the second injection means 100 is vertically opposed to the distance sensor unit 400, the second injection nozzle 122 is moved to the distance sensor unit 400 so that the absolute coordinates of the second injection nozzle 122 are calculated by the controller 500. [

Next, after the third injection nozzle 123 of the third injection means 100 is vertically opposed to the distance sensor unit 400, the third injection nozzle 123 is moved to the distance sensor unit 400 so that the absolute coordinates of the third injection nozzle 123 are calculated by the controller 500. [

Next, after the spraying unit 100 is positioned above the collecting unit 200, the first spraying nozzle 121 is aligned at a position where the biocompatible material is sprayed on the collecting unit 200 , And automatically aligns the initial position so as to be spaced apart from the surface of the collecting unit 200 by a predetermined height based on the absolute coordinates of the first injection nozzle 121 calculated through the above process.

When the cell culture supporting body S1 of the first layer is formed by the first injection nozzle 121, the second injection nozzle 122 is arranged at a position where the injection of the first injection nozzle 121 is completed The initial position is automatically aligned so as to be spaced apart from the cell culture support S1 of the first layer by a certain height based on the absolute coordinates of the second injection nozzle 122. [

When the cell culture support S2 of the second layer is formed by the second injection nozzle 122, the third injection nozzle 123 is arranged at a position where the injection of the second injection nozzle 122 is completed The initial position is automatically aligned so as to be spaced apart from the cell culture support S of the second layer by a predetermined height based on the absolute coordinates of the third injection nozzle 123 to form a cell culture support S3 of the third layer .

Next, the above-described procedure is repeated several times according to the number of the cell culture support (S) to be produced to complete the cell culture support (S).

Likewise, when the cell culture support S having different materials is alternately formed in a single layer as shown in FIG. 10, any one of the injection nozzles 120 of the injection means 100 in the region where the biocompatible material is varied The initial position is automatically aligned.

Fig. 11 shows three types of cell culture supporters prepared by simultaneously spraying three different materials through three injection means.

At this time, in the method of manufacturing a cell culture support of the present invention, three kinds of cell culture supports are manufactured at the same time in the state where all the three injection nozzles 120 are aligned in the initial positions through a) through e).

As described above, the method of manufacturing a cell culture support may be such that a plurality of cell culture supports are simultaneously produced through a plurality of the injection means, and each cell culture support to be manufactured may be made of different kinds of biocompatible materials , And biocompatible materials of the same kind.

On the other hand, in the case of a multi-layer structure in which a cell culture support S having different materials is formed for each layer as shown in FIG. 9, any one of the injection means 100 The initial position is automatically aligned in consideration of the height of each layer.

At this time, in the method for producing a cell culture support of the present invention, a) to e) must be performed before the cell culture support (S) is prepared. So that the operation can be accurately performed even when the position of the injection nozzle 120 is shaken or changed during the process.

the separation distance between the collecting unit 200 and the injection nozzle 120 is set to be different according to the diameter of the injection nozzle 120 in the step of aligning the initial position of the injection nozzle 120 When the diameter of the injection nozzle 120 is 300 mu m, it is spaced 300 mu m from the collector 200. When the diameter of the injection nozzle 120 is 500 mu m, it may be spaced 500 mu from the collector 200 have.

As shown in FIGS. 9 to 10, one composite cell culture support made of various materials is capable of changing physical properties in a cell culture support according to the characteristics of different materials, compared with a conventional cell culture support , It is possible to change the decomposition rate, so that the applicable fields can be further diversified.

Example 5

In Embodiment 5, a method of manufacturing a cell culture support using the apparatus for manufacturing a multi-nozzle type cell culture support 1 of the present invention will be described with reference to FIGS. 6 to 8. FIG.

The cell culture support manufacturing method of the present invention may further include a focusing step A1, a distance measuring step A2, and a distance calculating step A3 between steps d) and e).

In addition, the method for manufacturing a cell culture support of the present invention may further comprise the steps of: f) between the steps d) and e), the firing means is transported in the x-, y-, and z- A focusing step (A1) in which each is positioned so as to focus the CCD camera; g) a transfer distance measuring step (A2) in which the transfer distance of each of the injection nozzles (120) is measured by the control unit (500) after the CCD camera is focused; h) calculating a separation distance (A3) for calculating a distance between the injection nozzle (120) and the reference point (610) by the controller (500) ; Can be performed.

The apparatus for manufacturing a cell culture support according to the present invention comprises a focusing step of feeding the injecting means in the x-axis, the y-axis and the z-axis so that each of the nozzle portions is successively positioned at the reference point 610 to focus the CCD camera A1).

It is preferable that the steps f) to h) are generally performed once at the initial setting of the cell culture support manufacturing apparatus.

At this time, the controller 500 measures the distances of the injection nozzles 120 from the original position to the reference point 610, and calculates the distances d1, d2 between the adjacent injection nozzles 120 according to the measured distance, d2). Then, the position of each of the injection nozzles 120 is controlled according to the calculated distance so that one end of the plurality of injection nozzles 120 is positioned at a predetermined position of the collecting unit 200.

In the apparatus for manufacturing a cell culture support of the present invention, the solution of the solution storage part 110 is injected into the collecting part 200 through the injection nozzle 120 positioned at a predetermined position to form a cell culture support S The step of causing the injection nozzle 120 to come to a certain position of the collecting part 200 and the step of spraying the solution to the collecting part 200 are repeated for the number of layers of the cell culture support S to be manufactured , A cell culture support (S) having a multi-layer structure can be produced.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It goes without saying that various modifications can be made.

1: Multi-nozzle type cell culture support manufacturing apparatus
S: Cell culture supporter
S1: cell culture support of the first layer
S2: cell culture support of the second layer
S3: cell culture support of the third layer
100: injection means
110:
111, 112 and 113: First to third solution storage parts
120: injection nozzle
121, 122, 123: First to third injection nozzles
130:
131, 132, 133: first to third heating parts
134: hollow
150: Support
151, 152, 153: first to third supports
160: Fixing means
161: rotation link 162: first fixing portion
163:
200: collecting section
210: Temperature control means
300: injection position adjusting section
310: x-axis conveying unit 320: y-axis conveying unit
330: z-
400: Distance sensor unit
500:
600: Position alignment means
610: Reference point

Claims (12)

In a multi-nozzle type cell culture support manufacturing apparatus,
And the solution is biocompatible material storage reservoir, and the spray nozzle for spraying the received supplied from the solution reservoir solution, heating to control the temperature of the solution stored in the solution reservoir portion, the when upper and the heating element A plurality of spraying means connected to the z-axis conveying portion through a support formed to include a first support and a second support for supporting and fixing means for fixing the solution storage portion and the injection nozzle to the support ;
A planar collecting unit provided below the injecting unit to collect a solution injected through the injecting nozzle of the injecting unit and having a temperature adjusting unit therein;
An injection position regulating unit formed to include an x-axis transfer unit and a y-axis transfer unit for transferring a plurality of the injection units at the same time in the x-axis and the y-axis, and a z-axis transfer unit for transferring the plurality of the injection units in the z- And
A distance sensor which is spaced apart from the collecting part by a predetermined distance and which measures a distance from the spraying part to a point where the end of the spraying nozzle contacts the z-axis direction;
A control unit for calculating an absolute coordinate of each of the injection nozzles through a vertical distance between the distance sensor unit and the injection nozzle end measured by the distance sensor unit, and controlling the injection position and the collection position; Respectively,
The fixing means
Axis direction, and both end portions are hinged to a third support base of the support protruding in the y-axis direction on a surface connected to the z-axis conveyance portion so as to be located on both sides in the x-axis direction of the heating portion, Shaped rotation link,
A first fixing part formed on a surface of the solution storage part opposite to the upper surface of the solution storage part in a state where the rotation link rotates and is parallel to the z axis and presses and fixes the solution storage part in the z-
And a second fixing part formed on the first support and pressing and fixing the solution storage part in the y axis direction .
The method according to claim 1,
The multi-nozzle type cell culture support
The distance sensor unit being spaced apart from the distance sensor unit by a predetermined distance,
And a position alignment means which is a CCD camera for focusing when the end portions of the respective injection nozzles are sequentially positioned at a reference point for measuring distances between the adjacent injection nozzles in the x and y axes A device for manufacturing a multi-nozzle type cell culture support.
3. The method of claim 2,
The control unit
Measuring an x and y axis transfer distance of each of the injection nozzles when the end of the injection nozzle is positioned at the reference point and the CCD camera is focused,
Wherein the distance between the adjacent injection nozzles is calculated through the distance of each of the injection nozzles and the position of the injection nozzle is adjusted through the x-axis transfer part and the y-axis transfer part.
The method according to claim 1,
The injection means
The spray nozzle is separately connected to the lower part of the solution storage part,
And a hollow portion into which the solution storing portion is inserted is formed in the heating portion.
delete A method of manufacturing a cell culture support using a multi-nozzle type cell culture support manufacturing apparatus according to any one of claims 1 to 4 ,
a) transferring the injection means in the x-axis and y-axis directions so that the end of one of the plurality of injection means faces the distance sensor unit vertically;
b) the injection nozzle is lowered to contact the distance sensor unit;
c) calculating a z-axis absolute coordinate of the injection nozzle by measuring a distance by which the injection nozzle contacts the distance sensor unit by the control unit;
d) performing steps a) to c) for the remaining injection means to calculate the absolute coordinates of each of the injection means; And
e) automatically aligning the initial position so that any one of the spraying nozzles of the spraying means is located at a position where the biocompatible material of the collecting portion is to be sprayed by transferring the spraying means in the x-axis, y-axis and z-axis; ≪ / RTI >
The method according to claim 6,
The method for manufacturing a multi-nozzle type cell culture support
wherein the separation distance between the collecting part and the injection nozzle is set differently according to the diameter of the injection nozzle in step e) so that the initial position of the injection nozzle is aligned.
The method according to claim 6,
The cell culture support preparation method comprises
Between step d) and step e)
f) focusing the x-axis, y-axis, and z-axis of the injector means so that each of the injection nozzles is positioned at a reference point so that the CCD camera is focused;
g) a transfer distance measurement step in which the CCD camera is focused and then the transfer distance of each of the injection nozzles is measured by the control unit; And
h) calculating a distance between neighboring injection nozzles by measuring the distance by which the injection nozzle contacts the reference point by the control unit; Lt; RTI ID = 0.0 > cell culture < / RTI > support.
9. The method of claim 8,
The cell culture support preparation method comprises
Wherein the steps f) to h) are performed at the initial setting of the cell culture support manufacturing apparatus.
The method according to claim 6,
The cell culture support preparation method comprises
Wherein a cell culture support having a multi-layer structure in which different biocompatible materials are injected for each layer is manufactured.
The method according to claim 6,
The cell culture support preparation method comprises
Wherein a cell culture support in which different biocompatible materials are alternately injected into the same layer is produced.
The method according to claim 6,
The cell culture support preparation method comprises
When a plurality of cell culture supports are simultaneously produced through a plurality of the injection means,
Wherein each cell culture support is different or made from the same biocompatible material.

Images