KR101525733B1 - Surgical kits for autologous collagen-induced chondrogenesis - Google Patents

Surgical kits for autologous collagen-induced chondrogenesis Download PDF

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Publication number
KR101525733B1
KR101525733B1 KR1020140024202A KR20140024202A KR101525733B1 KR 101525733 B1 KR101525733 B1 KR 101525733B1 KR 1020140024202 A KR1020140024202 A KR 1020140024202A KR 20140024202 A KR20140024202 A KR 20140024202A KR 101525733 B1 KR101525733 B1 KR 101525733B1
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South Korea
Prior art keywords
cartilage
syringe
atelocollagen
collagen
filled
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KR1020140024202A
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Korean (ko)
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김석중
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가톨릭대학교 산학협력단
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/14—Surgical saws ; Accessories therefor
    • A61B17/15—Guides therefor
    • A61B17/154—Guides therefor for preparing bone for knee prosthesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/34—Trocars; Puncturing needles
    • A61B17/3472—Trocars; Puncturing needles for bones, e.g. intraosseus injections
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/45—For evaluating or diagnosing the musculoskeletal system or teeth
    • A61B5/4514—Cartilage

Abstract

The present invention relates to a surgical kit for autologous collagen-induced chondrogenesis, comprising: a microdrill for perforating at least one hole on a subchondral bone which is exposed since cartilaginous tissue thereof is damaged and removed; a CO_2 gas injector for discharging carbon dioxide gas pressurized for making the ambient air of the subchondral bone dry; a suction means for sucking moisture around the perforated subchondral bone and discharging the sucked moisture to the outside; and a two-way injector including a first injector filled with fibrinogen, a second injector filled with an atelocollagen-thrombin mixture, and a Y-type mixing catheter for mixing the substances filled in the first and second injectors respectively at a mixing ratio of 1:1, and then discharging the mixture.

Description

[0001] Description [0002] Surgical kits for autologous collagen-induced chondrogenesis derived from self-

The present invention relates to a surgical kit for cartilage regeneration derived from magnetic collagen. More particularly, the present invention relates to an operation kit for cartilage regeneration using magnetic arthroscopy, which comprises removing cartilage damaged by arthroscopy, The present invention relates to a surgical kit for cartilage regeneration derived from magnetic collagen.

Cartilage refers to the connective tissue of white elasticity attached to the bones that make up the joints. It acts as a buffer to absorb the shocks generated during joint movement or to make the friction between joints very low.

The cartilage is often worn out as it gets older and causes degenerative arthritis. In addition, it can cause instantaneous rupture of the cartilage due to flexion or extension of the cartilaginous joints beyond the joints.

If the cartilage damage is too severe, the artificial joint replacement should be done. However, if the cartilage damage is limited to some extent, chondrogenesis restoring the damaged cartilage is more preferable. Such cartilage regeneration has been studied for a long time.

Until now, autologous chondrocyte implantation has been known to be the most effective cartilage regeneration in that it forms hyaline-like cartilage similar to supernatural bone rather than fibrocartilage (Non-Patent Documents 1 and 2 Reference).

However, autologous chondrocyte transplantation is problematic in that it requires two procedures because the chondrocytes must be harvested after culturing and cultured, and the cost of culturing chondrocytes is also very high.

 Knutsen G, Engebretsen L, Ludvigsen TC, et al. Autologous chondrocyte implantation compared with microfracture in the knee. A randomized trial. J Bone Joint Surg Am. 2004; 86 (3): 455-464.  Brittberg M, Lindahle, Nilsson A, et al. Treat-ment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med. 1994; 331 (14): 889-895.

Accordingly, it is an object of the present invention to provide a surgical kit for cartilage regeneration derived from magnetic collagen, which can solve the drawbacks of conventional autologous chondrocyte transplantation and complete cartilage regeneration only by a single procedure using arthroscopy .

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a surgical kit for cartilage regeneration derived from magnetic collagen, which comprises a micro drill for puncturing at least one hole in an exposed cartilage bone with a damaged cartilage tissue removed, A CO 2 gas injector for injecting pressurized carbon dioxide gas; suction means for sucking moisture around the perforated cartilage and discharging it to the outside; A first syringe filled with fibrinogen, a second syringe filled with an atelocollagen-thrombin mixture, and a Y-type mixed material mixed and discharged at a volume ratio of 1: 1 in the first and second syringes, And a two-way syringe including a catheter.

Further, it may further comprise absorption means for absorbing and removing residual moisture around the cartilage.

The mixing ratio of the atelocollagen-thrombin mixture packed in the second syringe is 9: 1 (atelocollagen: thrombin).

It is preferable that the atelocollagen is high purity collagen of type 1 in which telopeptide is removed by pepsin treatment of collagen obtained from animal dermis.

The pressure of the carbon dioxide discharged from the CO 2 gas injector is preferably 18 to 22 mmHg and the flow rate is preferably 17 to 23 L / min.

The present invention having the above-described configuration can complete cartilage regeneration by only a single procedure, since it does not require a procedure for collecting and culturing normal cartilage cells as in the conventional autologous chondrocyte transplantation. The collagen mixture has good shape and adhesion so that the effect of cartilage regeneration is greatly improved.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a view showing the overall structure of a surgical kit for cartilage regeneration derived from magnetic collagen according to the present invention;
FIG. 2 is an MRI photograph of a patient having undergone cartilage regeneration derived from magnetic collagen using the surgical kit of the present invention. FIG.

Hereinafter, preferred embodiments of a surgical kit 10 for cartilage regeneration derived from magnetic collagen according to the present invention will be described in detail with reference to the accompanying drawings. The present invention is not limited to the embodiments described below, but may be implemented in various other forms, and the present embodiment is not intended to be exhaustive or to limit the scope of the present invention to those skilled in the art. .

FIG. 1 shows a surgical kit 10 (hereinafter abbreviated as "surgical kit of the present invention") for cartilage regeneration derived from magnetic collagen according to the present invention.

As shown in FIG. 1, the surgical kit 10 of the present invention includes a micro drill 100, a CO 2 gas injector 200, a suction unit 300, and a two-way injector 400.

Here, all the components included in the surgical kit 10 of the present invention approach the surgical site through the portal provided at the surgical site while observing the arthroscope. Therefore, will be.

The micro drill 100 is for drilling at least one hole in the exposed lower cartilage bone with the damaged cartilage tissue removed, and it is preferable that the drill is bent at an angle of about 45 degrees for smooth operation.

The perforation of the cartilage bone with the micro drill 100 is intended to increase the contact area so that the injected atelocollagen mixture has a proper bonding force to the cartilage bone. The number of perforated holes may vary depending on the area of the exposed cartilaginous bone, and it is preferable to perforate the perforations to a depth of approximately 3 mm and a depth of 6 mm.

The CO 2 gas injector 200 is configured to eject pressurized carbon dioxide gas to form a dry atmosphere around cartilage where cartilage regeneration is performed.

The injection of carbon dioxide gas is mainly intended to form a dry atmosphere for injecting a later-described atelocollagen mixture to prevent the bonding force from dropping due to moisture when the artificial cartilage is formed. Further, To ensure that the space in which the collagen mixture is injected and to prevent gravity from flowing down the atelocollagen mixture until it is adequately hardened.

In order to perform the above function, the pressure of the carbon dioxide emitted from the CO 2 gas injector 200 is preferably 18 to 22 mmHg, and the flow rate is preferably 17 to 23 L / min.

Particularly, since carbon dioxide has been already proven to be harmless to human body as widely used in laparoscopic surgery, it is easy to apply to the present invention.

On the other hand, the suction means 300 is a means for sucking moisture around the perforated cartilage and discharging it to the outside to smoothly remove moisture along with the injection of the carbon dioxide gas.

It is also possible, if necessary, to suck and remove the residual moisture around the cartilage by means of an absorbing means 500, for example an absorbing means 500 such as a swab, and the absorbing means 500, such as a swab, It is inserted through the inside of the inserted tube.

The two-way syringe 400 includes a first syringe 410 filled with fibrinogen, a second syringe 420 filled with the atelocollagen-thrombin mixture, and a second syringe 420 filled with the respective materials filled in the first syringe 410, Type mixed catheter 430 for mixing and discharging the mixture at a volume ratio of 1: 1.

That is, the material filled in each of the syringes 410 and 420 of the two-way syringe 400 is mixed with the perforated cartilage and mixed around the perforated cartilage to regenerate the cartilage. Particularly, the fibrinogen, which is filled in the first syringe 410, The cartilage tissue regenerated by the coagulation multistage phenomenon caused by thrombin in the atelocollagen-thrombin mixture packed in the reservoir 420 maintains its shape stably for about 5 minutes.

Atelocollagen is a connective tissue protein that plays an important role for maintaining the morphology of tissues. It is preferable that the collagen obtained from animal dermis is treated with pepsin to remove telopeptide and is a high-purity type 1 collagen. This high purity type 1 collagen does not cause an immune response because it is non-immunogenic and has very similar tissue properties to normal cartilage tissue.

The volume ratio of atelocollagen to thrombin is preferably 9: 1 in the mixing ratio of the atelocollagen-thrombin mixture filled in the second syringe 420.

The follow-up results (retrospective analysis) of the patients who have undergone the self-collagen-derived cartilage regeneration using the surgical kit 10 of the present invention are briefly summarized as follows.

The cartilage defects in all patients were classified as ICRS grade 3 and 4 (International Cartilage Repair Society grade Ⅲ, Ⅳ).

The knee joint function score (Lysholm score), MRI observation score (MOCART score) and color technique (T2 mapping) evaluation were performed with statistical values of mean ± standard deviation to compare the state of the cartilage before and after the operation.

Evaluation of knee functional score improved from 51.7 ± 27.1 before surgery to 81.3 ± 24.6 (P <0.05) after 2 years.

In addition, the MRI score at 1 year postoperatively was 70.4 ± 20.2 (15 to 95), and the results were fairly good. "4A" and "4B" (Double-echo steady-state MRI).

The arrow labeled " 4A " in FIG. 2 indicates ICRS grade 4 knee cartilage damage, and according to the "4B" image after 12 months, the atelocollagen injected by the surgical kit of the present invention .

The analysis of the "4C" MRI image by the color scheme (T2 mapping) shows that the reproduced cartilage and its surrounding cartilage are 34 ± 6 (ms) and 36 ± 6 (ms) According to the "4D" image of dGEMRIC (Delayed Gadolinium-Enhanced MRI of Cartilage), which is a photographic technique to observe the biochemical changes of cartilage, 505 ± 147 (ms) and 560 ± 120 (ms) This means that the regenerated cartilage is a hyaline-like cartilage similar to supernatural bone and its composition is close to the normal proteoglycan component.

Therefore, according to the above evaluation results, it can be seen that the atelocollagen injected and molded by the surgical kit of the present invention is very effective for cartilage regeneration, and in particular, it has a disadvantage of conventional autologous chondrocyte transplantation, It is possible to solve the disadvantage that it is necessary and expensive and time consuming.

As described above, the surgical kit for cartilage regeneration derived from magnetic collagen according to the present invention has been described with reference to the drawings. However, the present invention is not limited to the embodiments and the drawings disclosed herein, It will be understood by those skilled in the art that various changes may be made therein without departing from the spirit and scope of the invention.

10: surgical kit 100: micro drill
200: CO 2 gas injector 300: Suction means
400: Two-way syringe 410: First syringe
420: second syringe 430: Y-type mixed catheter
500: absorption means

Claims (5)

The present invention relates to a surgical kit for cartilage regeneration derived from magnetic collagen,
A micro drill for drilling at least one hole in the exposed cartilage bone with the damaged cartilage tissue removed;
A CO 2 gas injector for injecting pressurized carbon dioxide gas to form a dry atmosphere around the cartilage;
Suction means for sucking moisture around the perforated cartilage and discharging it to the outside; And
A first syringe filled with fibrinogen, a second syringe filled with a mixture of atelocollagen and thrombin, and a Y type mixed catheter for mixing and discharging the respective materials filled in the first syringe and the second syringe at a volume ratio of 1: A two-way syringe;
Wherein said cartilage regenerating cartilage regenerating cartilage is a cartilage cartilage regenerating cartilage. The method according to claim 1,
Further comprising absorbing means for absorbing and removing residual moisture around the cartilage. The method according to claim 1,
Wherein the mixing ratio of the atelocollagen-thrombin mixture packed in the second syringe is 9: 1 (atelocollagen: thrombin) volume ratio. The method according to claim 1 or 3,
Wherein the atelocollagen is a type 1 collagen in which telopeptide is removed by pepsin treatment of collagen obtained from animal dermis. The method according to claim 1,
Wherein the pressure of the carbon dioxide emitted from the CO 2 gas injector is 18 to 22 mmHg and the flow rate is 17 to 23 L / min.
KR1020140024202A 2014-02-28 2014-02-28 Surgical kits for autologous collagen-induced chondrogenesis KR101525733B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101860883B1 (en) * 2017-12-19 2018-05-24 주식회사 더블유앤지 Balloon infuser with multi chamber

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060077327A (en) * 2004-12-30 2006-07-05 학교법인연세대학교 Biodegradable anti-adhesivie membrane for promoting tissue regeneration and its preparation method
JP2008504895A (en) * 2004-06-29 2008-02-21 スパイン・ウェイブ・インコーポレーテッド Method for treating disc defects and injuries
KR20110044616A (en) * 2009-10-23 2011-04-29 세원셀론텍(주) A cartilage repair constituent and manufacturing method thereof
KR20130135340A (en) * 2011-04-15 2013-12-10 가톨릭대학교 산학협력단 Composition and method for regeneration of connective tissue

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008504895A (en) * 2004-06-29 2008-02-21 スパイン・ウェイブ・インコーポレーテッド Method for treating disc defects and injuries
KR20060077327A (en) * 2004-12-30 2006-07-05 학교법인연세대학교 Biodegradable anti-adhesivie membrane for promoting tissue regeneration and its preparation method
KR20110044616A (en) * 2009-10-23 2011-04-29 세원셀론텍(주) A cartilage repair constituent and manufacturing method thereof
KR20130135340A (en) * 2011-04-15 2013-12-10 가톨릭대학교 산학협력단 Composition and method for regeneration of connective tissue

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101860883B1 (en) * 2017-12-19 2018-05-24 주식회사 더블유앤지 Balloon infuser with multi chamber

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