KR101504783B1 - Method for diagnosing of Alzheimer's disease using giant magneto resistance and magnetic bead-polyprotein complex for Alzheimer's disease - Google Patents
Method for diagnosing of Alzheimer's disease using giant magneto resistance and magnetic bead-polyprotein complex for Alzheimer's disease Download PDFInfo
- Publication number
- KR101504783B1 KR101504783B1 KR1020100096495A KR20100096495A KR101504783B1 KR 101504783 B1 KR101504783 B1 KR 101504783B1 KR 1020100096495 A KR1020100096495 A KR 1020100096495A KR 20100096495 A KR20100096495 A KR 20100096495A KR 101504783 B1 KR101504783 B1 KR 101504783B1
- Authority
- KR
- South Korea
- Prior art keywords
- disease
- magnetoresistive sensor
- alzheimer
- cell
- magnetic bead
- Prior art date
Links
Images
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Neurology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Neurosurgery (AREA)
Abstract
The present invention relates to a method for diagnosing Alzheimer's disease using a giant magnetoresistive sensor and a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease. The method for diagnosing Alzheimer's disease using the giant magnetoresistive sensor according to the present invention can easily diagnose Alzheimer's disease by a simple method using a giant magnetoresistive sensor instead of a conventional fluorescent material or genetic analysis, Since it can be mass-produced, it can be useful for monitoring and treating Alzheimer's disease.
Description
The present invention relates to a method for diagnosing Alzheimer's disease using magnetic resistance sensors and a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease.
Alzheimer's disease is the leading cause of dementia and is the most common degenerative neurological disease. In fact, progressive adult Alzheimer's disease is characterized by memory loss, language abilities, situational judgment, and degeneration of discernment. The nature of the symptoms that are often confused with older physiological disorders, the severity of these symptoms, and the age at which these symptoms occur varies from person to person. As a result, the exact pathogenesis or treatment of Alzheimer's disease is not known, and it is difficult to diagnose Alzheimer's disease early.
The pathological features of Alzheimer's disease include senile plaques, neurofibrilary tangles, and neuronal loss. The aggregated amyloid - beta protein, which accounts for most of the elderly spots, is thought to be a major cause of Alzheimer 's disease due to various experimental evidences.
On the other hand, there are many ways to diagnose Alzheimer's disease. Korean Patent Laid-Open No. 10-2009-0048192 discloses a diagnostic, preventive and therapeutic agent for dementia (Alzheimer's disease) and a screening method thereof. Specifically, Fc? RIIb and its mutant, Fc? RIIb extracellular domain protein, anti-Fc? RIIb antibody , Fc [gamma] RIIb specific peptides, and Fc [gamma] RIIb, and in particular siRNA, reduce the signal transduction, intracellular migration, neurotoxicity, apoptosis and memory loss by Fc [gamma] RIIb and A [beta], so diagnosis, prevention and treatment of Alzheimer's disease .
Korean Patent Laid-Open No. 10-2007-0073778 discloses a method for measuring β-amyloid in a sample of a biological sample such as blood and is applied to the diagnosis of Alzheimer's disease. Amyloid 1-42 and? -Amyloid 1-42 in the biological specimen are measured by an immunoassay using an antibody recognizing the terminal portion of the? -Amyloid 1-42 and the? -Amyloid 1-42 fragment having the C-terminal portion of? -Amyloid 1-42 Lt; RTI ID = 0.0 > Alzheimer ' s < / RTI >
Korean Patent Laid-Open No. 10-2009-0098941 also discloses methods and compositions that can be used for detecting Alzheimer's disease in mammals, particularly humans, and in particular, methods used in serum markers for Alzheimer's disease and diagnostic procedures . Since the above methods use optical or chemical methods, they are difficult to be commercialized because of difficulties in real-time diagnosis, high cost of diagnosis, and mass production of an Alzheimer's disease diagnostic kit.
Accordingly, an object of the present invention is to provide a method of detecting Alzheimer's disease by a simple and easy method by detecting amyloid-beta protein using a magnetoresistive sensor.
Another object of the present invention is to provide a magnetic bead-multiple protein complex for diagnosing Alzheimer's disease, which is pretreated so as to bind magnetic beads used for diagnosing Alzheimer's disease using magnetoresistive sensors to protein biomarkers .
Another object of the present invention is to provide a method for preparing a magnetic bead-multiple protein complex for diagnosing Alzheimer's disease, which is bound to a protein biomarker by binding magnetic beads used for diagnosing Alzheimer's disease using a magnetoresistive sensor to multiple proteins .
In order to solve the above-mentioned problems, the present invention provides a method for preparing a protein biomarker, comprising the steps of: pre-treating magnetic beads so as to bind to a protein biomarker inducing Alzheimer's disease; Disposing a cell to be diagnosed on the magnetoresistive sensor; Placing the pretreated magnetic bead on the cell to be diagnosed and applying an external magnetic field to magnetize the pretreated magnetic bead; And detecting the presence of Alzheimer's disease in the subject cell by detecting the magnetic field change according to whether the pretreated magnetic bead is coupled with the protein biomarker by the magnetoresistive sensor. Provides a diagnostic method.
The magnetic field change may be caused by a stray field generated in the magnetic bead when the magnetic bead is bound due to the existence of the protein biomarker.
The protein biomarker may be an amyloid-beta protein.
The magnetic beads may range in diameter from 50 nm to 5 mu m.
The pretreatment can be carried out by coating magnetic beads with streptavidin, binding with biotin, and binding poly (ethylene glycol).
The magnetoresistive sensor may be an anisotropic magnetoresistive thin film, a giant magnetoresistive thin film, a tunnel magnetoresistive thin film, or the like.
The magnetoresistive sensor may include a free layer, a spacer, a pinned layer, and a pinning layer.
The magnetoresistive sensor may be in the shape of a cross or a rod.
The magnetoresistive sensor may be protected by an oxide or nitride thin film layer.
The cells may be brain cells, olfactory cells, taste cells, or visual cells capable of accumulating amyloid-beta protein.
The magnetization may be performed by applying an external magnetic field to the surface of the magnetoresistive sensor in a vertical or horizontal direction.
The present invention also relates to a method for producing a biomolecule comprising the steps of coating a magnetic bead with streptavidin, wherein the streptavidin is bound to biotin, and the biotin is linked to a protein biomarker, Diagnostic magnetic bead-multiprotein complex.
The protein biomarker is an amyloid-beta protein.
The linker may be polyethylene glycol (poly (ethylene glycol)) or the like.
The present invention also provides a method of preparing a magnetic bead, comprising: coating a magnetic bead with streptavidin; Binding the streptavidin-coated magnetic beads with biotin; And binding the biotin to a linker, wherein the linker is bound to a protein biomarker. The present invention also provides a method for producing a magnetic bead-multiple protein complex for diagnosing Alzheimer's disease.
The method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention can easily diagnose Alzheimer's disease by a simple method using a giant magnetoresistive sensor instead of a conventional fluorescent material or gene analysis.
In addition, the method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention can produce a large amount of biosensor for diagnosis of Alzheimer's disease and can be useful for monitoring and treating Alzheimer's disease.
FIG. 1 is a flow chart showing a method for diagnosing Alzheimer's disease using a magnetoresistive sensor according to the present invention.
FIG. 2 is a process flow diagram illustrating a method for producing a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease according to the present invention.
3 is a schematic diagram showing a giant magnetoresistive sensor for diagnosing Alzheimer's disease according to the present invention.
FIG. 4 is a schematic view showing a structure of a giant magnetoresistive thin film for diagnosing Alzheimer's disease according to the present invention.
5 is a schematic view showing the magnetic field direction of magnetic beads when the magnetic beads are magnetized in the direction perpendicular to the surface of the magnetoresistive sensor.
6 is a schematic view showing the magnetic field direction of magnetic beads when the magnetic beads are magnetized in the horizontal direction with respect to the surface of the magnetoresistive sensor.
7 is a schematic diagram showing a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease according to the present invention.
8 is a schematic diagram illustrating a method for diagnosing Alzheimer's disease using a giant magnetoresistive sensor according to the present invention.
Hereinafter, the present invention will be described in detail.
In the method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention, a process of pretreating a magnetic bead is required so as to be able to bind to a protein biomarker causing Alzheimer's disease.
At this time, the protein biomarker may be amyloid-beta protein, and the protein biomarker may be a cell existing on the surface.
The magnetic beads may be coated with streptavidin for selective binding to amyloid-beta protein known to cause Alzheimer's disease, and then selectively bound to biotin and then mixed with biotin such as poly (ethylene glycol) A pretreatment process is performed so as to bind amyloid-beta protein using a linker. The magnetic bead preferably has a diameter in the range of 50 nm to 5 mu m. If the diameter of the magnetic beads is less than 50 nm, it is difficult to sense the stray field generated by the magnetic beads. If the magnetic beads have a diameter of more than 5 탆, bonding with the amyloid-beta existing on the cell surface There is a difficult problem.
Through this pretreatment, magnetic beads can selectively bind amyloid-beta protein. That is, in the case of Alzheimer's disease, it binds to magnetic beads because it contains amyloid-beta protein, and when it is a normal cell, it does not bind to magnetic beads.
In the method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention, a step of disposing the cells to be diagnosed on the magnetoresistive sensor is performed.
The magnetoresistive sensor may be an anisotropic magnetoresistive thin film, a giant magnetoresistive thin film, a tunnel magnetoresistive thin film, or the like. The giant magnetoresistive thin film may be a spin valve thin film capable of obtaining a large resistance change of 5 to 10% Can be used.
The magnetoresistive sensor may include a free layer, a spacer, a pinned layer, and a pinning layer, and may have a cross shape or a rod shape.
The magnetoresistive sensor may be protected by an oxide or nitride thin film layer, and the cell disposed on the magnetoresistive sensor is preferably a brain cell, olfactory cell, taste cell, or visual cell capable of accumulating amyloid-beta protein .
In the method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention, the pre-processed magnetic bead is placed on the cell to be diagnosed, and magnetization is performed by applying an external magnetic field.
The preprocessed magnetic bead magnetizes an external applied magnetic field in a direction perpendicular or horizontal to the surface of the magnetoresistive sensor, and a fine stray field is generated from the magnetic bead due to magnetization.
In the method for diagnosing Alzheimer's disease using the magnetoresistive sensor according to the present invention, a magnetic field change depending on whether the pretreated magnetic beads are coupled with the protein biomarker is detected by the magnetoresistive sensor, and Alzheimer's disease And diagnose Alzheimer's disease.
The magnetic beads combined with the amyloid-beta protein can detect the change of the magnetic field due to the stray magnetic field generated by the magnetization by the external magnetic field by using the magnetoresistive sensor and can detect the presence of the amyloid-beta protein. Therefore, the presence or absence of Alzheimer's disease can be determined by the magnetoresistance value of the magnetoresistive sensor by the change of the applied voltage and the output voltage supplied to the magnetoresistive sensor. At this time, as the number of amyloid-beta proteins increases, the number of bonded magnetic beads also increases, so that the magnetoresistance value changes linearly.
In addition, the present invention relates to a method for producing a biomolecule comprising the steps of coating streptavidin around magnetic beads, linking streptavidin with biotin and linking the biotin with a protein biomarker, Diagnostic magnetic bead-multiprotein complex.
The protein biomarker is preferably an amyloid-beta protein, and the linker is preferably polyethylene glycol (poly (ethylene glycol)).
The present invention also provides a method of preparing a magnetic bead, comprising: coating a magnetic bead with streptavidin; Binding the streptavidin-coated magnetic beads with biotin; And binding the biotin to a linker, wherein the linker is bound to a protein biomarker. The present invention also provides a method for producing a magnetic bead-multiple protein complex for diagnosing Alzheimer's disease.
≪ Example 1 >
Magnetic bead Preprocessing step ( S100 )
Magnetic beads with a diameter of about 100 nm were pretreated to detect protein biomarkers that cause Alzheimer's disease. The pretreatment was performed with streptavidin around the magnetic beads, and then the streptavidin was bound to biotin. The biotin was bound to a linker, polyethylene glycol, in order to bind the amyloid-beta protein biomarker. Through the pretreatment, the magnetic beads selectively bind to the amyloid-beta protein. In the case of Alzheimer's disease, the magnetic beads bind to magnetic beads because they contain amyloid-beta protein. When they are normal cells, they do not bind to magnetic beads.
Cell placement stage for diagnosis ( S120 )
A giant magnetoresistive sensor with a spin valve structure protected by an oxide thin film layer placed a brain cell capable of accumulating amyloid-beta protein on the upper surface thereof. The giant magnetoresistive sensor includes a free layer, a spacer layer ), A pinned layer, and a pinning layer.
Magnetic bead Magnetization phase ( S130 )
The pre-processed magnetic bead was placed on the giant magnetoresistive sensor and an external magnetic field was applied. The preprocessed magnetic bead applied an external magnetic field in a direction perpendicular to the surface of the magnetoresistive sensor, and a microscopic stray magnetic field was generated in the magnetic bead magnetized by the external magnetic field.
Alzheimer's disease presence determination step ( S140 )
The magnetic beads combined with the amyloid-beta protein can detect the change of the magnetic field due to the stray magnetic field generated by the magnetization due to the external magnetic field by the magneto-resistive sensor. The magnetoresistance value in the presence of the normal cell and the magnetoresistance value of the Alzheimer's cell And the magnetoresistance value at the time of existence was compared to diagnose Alzheimer's disease. At this time, as the number of amyloid-beta proteins increased, the number of bound magnetic beads also increased. Therefore, the magnetoresistance value changed linearly.
≪ Example 2 >
Streptavidin is bound to biotin (S210) after streptavidin is coated around the magnetic bead having a diameter of 100 nm (S210). To bind the biotin with amyloid-beta protein, streptavidin is coated with polyethylene glycol (S220) to prepare a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease.
The present invention will now be described in detail with reference to the accompanying drawings. Hereinafter, a repeated description, a known function that may obscure the gist of the present invention, and a detailed description of the configuration will be omitted. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Accordingly, the shapes and sizes of the elements in the drawings and the like can be exaggerated for clarity.
FIG. 1 is a flow chart showing a method for diagnosing Alzheimer's disease using a magnetoresistive sensor according to the present invention.
FIG. 2 is a process flow diagram illustrating a method for producing a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease according to the present invention.
3 is a schematic diagram showing a giant magnetoresistive sensor for diagnosing Alzheimer's disease according to the present invention.
Referring to FIG. 3, a layer of
FIG. 4 is a schematic view showing a structure of a giant magnetoresistive thin film for diagnosing Alzheimer's disease according to the present invention.
4, the structure of the giant magnetoresistive sensor used in the present invention is a spin valve composed of a free layer, a spacer, a pinned layer, and a pinning layer. Structure.
This spin valve structure is suitable for measuring the magnetoresistance value of magnetic particles combined with amyloid-beta protein in a giant magnetoresistive sensor because it has the advantage of obtaining a large resistance change of 5 to 10% even in a small magnetic field.
The giant magnetoresistive sensor used in the present invention can measure a change in an external applied magnetic field due to a microscopic stray field generated from spherical magnetic particles having a size of several 탆 to several 탆, The resistance change characteristic of the magnetoresistive sensor can be represented by an electrical output signal.
5 and 6 are schematic views showing magnetic field directions of the magnetic beads when the magnetic beads are magnetized in a direction perpendicular or horizontal to the surface of the magnetoresistive sensor.
5 and 6, the direction of the magnetic field applied from the outside can be applied to the surface of the giant magnetoresistive sensor in the vertical direction or in the horizontal direction, and the magnetic bead can be applied to the giant magnetoresistive sensor The stray field generated in the magnetic beads at this time affects the magnetization of the giant magnetoresistive sensor, thereby changing the resistance characteristic of the giant magnetoresistive sensor, and the magnetic bead The characteristics of the output voltage caused by the output voltage vary.
7 is a schematic diagram showing a magnetic bead-multiprotein complex for diagnosing Alzheimer's disease according to the present invention.
Referring to FIG. 7, magnetic beads (or magnetic particles, 300) used in the present invention are coated with
Through such a binding method, the magnetic beads selectively bind to the amyloid-beta protein. In the case of cells containing the amyloid-beta protein that induces Alzheimer's disease, the magnetic beads bind to the magnetic beads, and the amyloid- In normal cells, it does not bind to magnetic beads.
8 is a schematic diagram illustrating a method for diagnosing Alzheimer's disease using a giant magnetoresistive sensor according to the present invention.
Referring to FIG. 8, the magnetoresistive sensor senses a change in magnetic field due to a table dielectric flow generated in a magnetic bead after disposing a cell to be diagnosed on a magnetoresistive sensor and applying an external magnetic field to magnetize the magnetic bead. It is possible to detect the presence of the beta protein. Therefore, it is possible to judge Alzheimer's disease by comparing the magnetoresistance value in the presence of normal cells and the magnetoresistance value in the presence of Alzheimer's disease cells using a giant magnetoresistive sensor. Also, as the number of amyloid-beta proteins increases, the number of bound magnetic beads increases, and thus the magnetoresistance value changes linearly. As a result, it is possible to diagnose not only the early diagnosis of Alzheimer's disease but also the progress of Alzheimer's disease by measuring the magnetoresistance value changed by magnetic beads combined with amyloid-beta protein.
The present invention has advantages in that it can be easily and easily diagnosed using a conventional fluorescent material or gene analysis or comparison method, and can be commercialized as a biosensor for diagnosis of Alzheimer's disease.
As described above, an optimal embodiment has been disclosed in the drawings and specification. Although specific terms have been employed herein, they are used for purposes of illustration only and are not intended to limit the scope of the invention as defined in the claims or the claims. Therefore, those skilled in the art will appreciate that various modifications and equivalent embodiments are possible without departing from the scope of the present invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.
100: electrode (Ta: Au)
110: passivation (oxide)
120: Giant magnetoresistive sensor (GMR sensor)
130: substrate
300: magnetic bead
310: Streptavidin < RTI ID = 0.0 >
320: Biotin
330: Polyethylene glycol (Polyethylene glycol)
340: Amyloid-beta protein (Amyloid-beta)
Claims (16)
Disposing a cell to be diagnosed on the magnetoresistive sensor;
Placing the pretreated magnetic bead on the cell to be diagnosed and applying an external magnetic field to magnetize the pretreated magnetic bead; And
Detecting the presence of Alzheimer's disease in the cell to be diagnosed by detecting the magnetic field change according to whether or not the pre-treated magnetic bead is coupled with the protein biomarker by the magnetoresistive sensor, and diagnosing Alzheimer's disease using the magnetoresistive sensor The method comprising:
Wherein the protein biomarker is an amyloid-beta protein,
The pretreatment is carried out by coating magnetic beads with streptavidin, binding with biotin, and binding poly (ethylene glycol)
Wherein the magnetoresistive sensor is an anisotropic magnetoresistive thin film or a tunnel magnetoresistive thin film.
Wherein the magnetic field change is caused by a stray field generated in the magnetic bead when the magnetic bead is present due to the presence of the protein biomarker, and information for diagnosing Alzheimer's disease using the magnetoresistive sensor is provided How to.
Wherein the magnetic beads have diameters in the range of 50 nm to 5 mu m.
Wherein the magnetoresistive sensor includes a free layer, a spacer, a pinned layer, and a pinning layer. The magnetoresistive sensor includes information for diagnosing Alzheimer's disease using the magnetoresistive sensor, How to provide.
Wherein the magnetoresistive sensor has a shape of a cross or a rod. The method for providing information for diagnosing Alzheimer's disease using the magnetoresistive sensor.
Wherein the magnetoresistive sensor is protected by an oxide or nitride thin film layer. ≪ RTI ID = 0.0 > 11. < / RTI >
Wherein the cell is a brain cell, olfactory cell, taste cell or visual cell capable of accumulating amyloid-beta protein.
Wherein the magnetization is performed by applying an external magnetic field to the surface of the magnetoresistive sensor in a vertical or horizontal direction. ≪ RTI ID = 0.0 > 15. < / RTI >
Disposing a cell to be diagnosed on the magnetoresistive sensor;
Placing the pretreated magnetic bead on the cell to be diagnosed and applying an external magnetic field to magnetize the pretreated magnetic bead; And
Detecting the presence or absence of Alzheimer's disease in the cell to be diagnosed by detecting the magnetic field change depending on whether the pretreated magnetic bead is coupled with the protein biomarker by the magnetoresistive sensor, As a method for diagnosing a disease,
Wherein the protein biomarker is an amyloid-beta protein,
The pretreatment is carried out by coating magnetic beads with streptavidin, binding with biotin, and binding polyethylene glycol,
Wherein the magnetoresistive sensor is an anisotropic magnetoresistive thin film or a tunnel magnetoresistive thin film.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/078,312 US20110244484A1 (en) | 2010-04-05 | 2011-04-01 | Method of diagnosing alzheimer's disease using giant magnetoresistance sensor and magnetic bead-polyprotein complex for diagnosing alzheimer's disease |
| JP2011082594A JP2011221017A (en) | 2010-04-05 | 2011-04-04 | Diagnosis method of alzheimer's disease using magnetoresistive sensor, and magnetic bead-polyprotein complex for diagnosing alzheimer's disease |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20100030815 | 2010-04-05 | ||
| KR1020100030815 | 2010-04-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20110112177A KR20110112177A (en) | 2011-10-12 |
| KR101504783B1 true KR101504783B1 (en) | 2015-03-23 |
Family
ID=45028065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020100096495A KR101504783B1 (en) | 2010-04-05 | 2010-10-04 | Method for diagnosing of Alzheimer's disease using giant magneto resistance and magnetic bead-polyprotein complex for Alzheimer's disease |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2011221017A (en) |
| KR (1) | KR101504783B1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9541561B2 (en) | 2012-06-14 | 2017-01-10 | Electronics And Telecommunications Research Institute | Method for diagnosing Alzheimer's disease using biomaterial |
| US9645141B2 (en) | 2012-11-30 | 2017-05-09 | Korea Institute Of Science And Technology | Method for diagnosing biomarkers and biomarker diagnosis kit |
| KR101979256B1 (en) * | 2012-12-26 | 2019-08-28 | 한국전자통신연구원 | Analysis device and analysis method using the device |
| JP6982099B2 (en) | 2018-07-27 | 2021-12-17 | ゼプト ライフ テクノロジー, エルエルシーZepto Life Technology, Llc | Systems and methods for detecting test substances in the detection of biomarkers by GMR |
| CN111722165B (en) | 2019-03-20 | 2023-02-28 | Tdk株式会社 | Magnetic sensor, detection device, and detection system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100949804B1 (en) * | 2007-12-14 | 2010-03-30 | 한국전자통신연구원 | Magnetic field detection device |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04240561A (en) * | 1991-01-25 | 1992-08-27 | Nkk Corp | Method and apparatus for magnetic flaw detection of wire rope |
| DE19952955A1 (en) * | 1999-11-03 | 2001-05-17 | Acgt Progenomics Ag | Methods for the characterization and separation of molecular associates |
| US7106051B2 (en) * | 2001-12-21 | 2006-09-12 | Koninklijke Philips Electronics, N.V. | Magnetoresistive sensing device, system and method for determining a density of magnetic particles in fluid |
| DE602004012330T2 (en) * | 2004-04-29 | 2009-03-19 | Marc Ramael | METHOD AND KIT FOR DETECTING COMPONENTS IN A SAMPLE |
| JP2007170839A (en) * | 2005-12-19 | 2007-07-05 | Asahi Kasei Corp | Reagent with marker, and analyzing device and method using same |
| JP4801447B2 (en) * | 2006-01-13 | 2011-10-26 | 旭化成株式会社 | Measuring apparatus and measuring method using magnetic sensor |
| JP2010504515A (en) * | 2006-09-20 | 2010-02-12 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Sensor device and method for detecting particles |
| JP2009025193A (en) * | 2007-07-20 | 2009-02-05 | Canon Inc | Sensing method |
| JP2009250926A (en) * | 2008-04-10 | 2009-10-29 | Canon Inc | Magnetic sensor and detection method of magnetic particle |
-
2010
- 2010-10-04 KR KR1020100096495A patent/KR101504783B1/en not_active IP Right Cessation
-
2011
- 2011-04-04 JP JP2011082594A patent/JP2011221017A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100949804B1 (en) * | 2007-12-14 | 2010-03-30 | 한국전자통신연구원 | Magnetic field detection device |
Non-Patent Citations (4)
| Title |
|---|
| 논문1: ANGEWANDTE CHEMIE INTERNATIONAL EDITION * |
| 논문1: ANGEWANDTE CHEMIE INTERNATIONAL EDITION* |
| 논문2: JOURNAL OF ALZHEIMER'S DISEASE * |
| 논문2: JOURNAL OF ALZHEIMER'S DISEASE* |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2011221017A (en) | 2011-11-04 |
| KR20110112177A (en) | 2011-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101504783B1 (en) | Method for diagnosing of Alzheimer's disease using giant magneto resistance and magnetic bead-polyprotein complex for Alzheimer's disease | |
| Zhu et al. | Antibody‐mimetic peptoid nanosheet for label‐free serum‐based diagnosis of Alzheimer's disease | |
| Meyer et al. | CRP determination based on a novel magnetic biosensor | |
| EP2390651B1 (en) | GMR biosensor with enhanced sensitivity | |
| JP6422935B2 (en) | Magnetic tunnel junction sensor and method of using the same | |
| EP2579042B1 (en) | Method for detecting Aß-specific antibodies in a biological sample | |
| CN101868286A (en) | Analyte detection with magnetic sensors | |
| US8815610B2 (en) | Magnetic nanoparticle detection across a membrane | |
| CN108474779B (en) | Protein and autoantibody biomarkers for diagnosis and treatment of lung cancer | |
| Zhao et al. | Label-free detection of Alzheimer’s disease through the ADP3 peptoid recognizing the serum amyloid-beta42 peptide | |
| CN110869387A (en) | Method for quantifying autoantibody | |
| US20110244484A1 (en) | Method of diagnosing alzheimer's disease using giant magnetoresistance sensor and magnetic bead-polyprotein complex for diagnosing alzheimer's disease | |
| CN107923877B (en) | Apparatus and method for improving sensitivity of magnetic sensor | |
| US8802057B2 (en) | Force-induced magnetization contrast for diagnosis and imaging | |
| JP2015127694A (en) | One-chip assay internal correction method and method of measuring target substance using the same | |
| US20140057364A1 (en) | Method of diagnosing alzheimers disease using saliva | |
| US20210041434A1 (en) | Systems and Methods for Measuring Binding Kinetics of Analytes in Complex Solutions | |
| CN113646635A (en) | Method for generating a circulating analyte profile and apparatus for performing the method | |
| Li et al. | Biomarkers identification and detection based on GMR sensor and sub 13 nm magnetic nanoparticles | |
| CN111722165B (en) | Magnetic sensor, detection device, and detection system | |
| KR102328709B1 (en) | peptide for detecting small molecular weight amyloid beta and use thereof | |
| JP2009128234A (en) | Material detecting device and material detecting method | |
| US20190120794A1 (en) | Chemical sensor | |
| Li et al. | Biomarker Quantification in Unprocessed Human Sera by Using A New Nanomagnetic Protein Assay | |
| IMPEDANCE | that a human-like APP processing leads to various mechanisms occurring independently of plaques genesis and reinforce the importance to focus research on early events of AD. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| LAPS | Lapse due to unpaid annual fee |