KR101499908B1 - Composition comprising herbs extract for tenderizer of meat and manufacturing method of the same - Google Patents
Composition comprising herbs extract for tenderizer of meat and manufacturing method of the same Download PDFInfo
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- KR101499908B1 KR101499908B1 KR1020120067077A KR20120067077A KR101499908B1 KR 101499908 B1 KR101499908 B1 KR 101499908B1 KR 1020120067077 A KR1020120067077 A KR 1020120067077A KR 20120067077 A KR20120067077 A KR 20120067077A KR 101499908 B1 KR101499908 B1 KR 101499908B1
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- meat
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- powder
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- 235000012141 vanillin Nutrition 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
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- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
- A23L27/12—Natural spices, flavouring agents or condiments; Extracts thereof from fruit, e.g. essential oils
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
- A23L13/42—Additives other than enzymes or microorganisms in meat products or meat meals
- A23L13/426—Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/70—Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor
- A23L13/72—Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L23/00—Soups; Sauces; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2200/00—Function of food ingredients
- A23V2200/02—Antioxidant
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Abstract
본 발명은 육류의 연육 및 소화능을 향상시키는 생약제 추출물 함유 연육제 및 그 제조방법에 관한 것으로, 고기의 육질을 연육 시킴으로서 저작 및 소화능을 향상시키는 연육제를 제공하는 효과를 나타내고 단백질 분해 및 항산화 활성이 있는 천연물을 첨가함으로써 육류가공 및 요리상에서 생성되는 산화물과 아질산염을 제거하는 매우 뛰어난 효과가 있는 연육소스를 제공하는 뛰어난 효과가 있다.The present invention relates to a flavor enhancer containing an extract of a herb medicine which improves the meat meat's digestive and digesting ability and a method for producing the same. There is an excellent effect of providing a meat sauce having an extremely excellent effect of removing the oxides and nitrites produced in the meat processing and cooking by adding the natural material having the activity.
Description
본 발명은 육류의 연육 및 소화능을 향상시키는 연육제 및 그 제조방법에 관한 것으로 더욱 상세하게는 천연 단백질 분해, 항산화 및 아질산염 제거 활성이 우수한 생약제를 이용하여 육류가공 및 요리상에서 생성되는 산화물과 아질산염을 제거하는 생약제 추출물 함유 연육제 및 그 제조방법에 관한 것이다.TECHNICAL FIELD The present invention relates to a frying agent which improves the meat softening and digestibility and a preparation method thereof, and more particularly to a frying agent which is excellent in natural protein degradation, antioxidation and nitrite removal activity, And a method for producing the same.
생활수준이 향상됨에 따라 음식 문화도 다양하게 발전해왔고 경제성장과 더불어 국민 소득이 향상됨에 따라 육류의 소비가 증가하고 있다. 단백질의 섭취는 필수적이지만 사회가 점점 고령화 시대로 접어들면서 노인들의 육류섭취는 저작 및 소화력의 문제로 섭취가 힘든 실정이다. 또, 우리나라 식단에서 고기가 차지하는 비율이 높아짐에 따라 노인들과 어린이를 위해 연육에 대한 관심이 날로 높아지고 있다. As the standard of living has improved, the food culture has been diversified, and the consumption of meat has increased as the national income has improved along with the economic growth. Protein intake is essential, but as the age of society is becoming more and more aged, the consumption of meat by the elderly is difficult to ingest because of the problems of mastication and digestive power. In addition, as the proportion of meat in the Korean diet increases, there is a growing interest in agriculture for elderly people and children.
그러나 육류를 연하게 하기 위한 연육제로서 현재 시판되고 있는 식품첨가물은 주로 파파인(파파야) 또는 브로멜린(파인애플) 등 열대 과일에서 추출한 추출물 이며 수입에 의존하고 있는 실정이며 아직 가정에서의 연육제의 사용은 제한적이라고 할 수 있겠다. 또한 상기와 같이 시판되는 연육제들은 주로 스테이크 등의 서양조리에 적절한 것으로 우리나라에서처럼 간장, 설탕 등의 조미료가 들어가는 요리에서는 잘 맞지 않는 문제점도 있다.
However, as food additives for softening meat, the currently marketed food additives are mainly extracts from tropical fruits such as papaya (papaya) or bromelin (pineapple), and they depend on imports. Is limited. In addition, the above-described softening agents are suitable for Western cooking such as steak, and they are not suitable for dishes containing seasonings such as soy sauce and sugar as in Korea.
상기, 식품첨가물의 사용 목적은 식품의 품질보존 및 맛, 향기, 색상 등의 개량이다. 특히 식품의 품질보존을 위한 보존료는 식품의 변질과 부패, 화학적 변화를 방지하여 영양성분과 신선도를 유지시키기 위해 사용된다. 상기 보존료의 작용은 부패미생물의 발육저지를 위한 정균 및 살균작용과 식품에 함유된 지방의 산패억제, 산화반응에 의한 부패방지 등이 있으며 이로써 식품안정성을 확보하고 저장성과 상품성을 증대시킨다. The purpose of the food additives is to preserve the quality of the food, and to improve taste, aroma, and color. In particular, preservatives for preservation of food quality are used to prevent deterioration, decay, and chemical changes in food, thereby maintaining nutritional and freshness. The function of the above preservatives is to inhibit the growth of microorganisms and bacteriostatic action to inhibit the growth of microorganisms, to inhibit the acidity of fat contained in food, and to prevent corruption due to oxidation reaction, thereby securing food stability and enhancing storage and commerciality.
특히, 식품에서의 지방 산패억제와 산화반응 억제를 위한 첨가물은 항산화제가 대표적으로 사용되며, 상기 식품첨가물로서의 항산화제는 토코페롤, 아스코르브산, 몰식자산프로필(PG), 디부틸하이드록시톨루엔(BHT), 부틸하이드록시아니졸 (BHA), 에리소르브산, 구아약지(guaiac resin), 노르디하이드로구아야레트산 등이 있다. In particular, antioxidants as food additives are tocopherols, ascorbic acid, propyl gallate (PG), dibutylhydroxytoluene (BHT), and the like. Butyl hydroxyanisole (BHA), erisorbic acid, guaiac resin, and nordihydroguaiaretic acid.
한편, 육류를 가공하여 연하게 하는 방법은 가열, 가압, 단백질 분해효소처리 등의 방법이 있다. 그러나 상기 방법들은 변색, 변질, 과다 연육 등의 문제점들이 제기되어 식품첨가물을 처리하여 식육을 연육시키는 방법들이 개발되고 있다. 그리고 육류가공시 단백질 변성에 의하여 발생되는 발암물질인 니트로소아민의 제거와 지방의 산패 및 변질을 억제하기 위한 항산화제의 처리가 요구되고 있다. 따라서, 단백질 분해활성이 우수하여 연육효과가 있고, 비타민, 폴리페놀 등의 항산화 생리활성물질이 풍부한 과실류 및 생약제를 이용한 천연 연육제 개발이 절실히 필요하다.
On the other hand, there is a method such as heating, pressurization, proteolytic enzyme treatment, etc. for processing meat and softening it. However, the above methods have raised problems such as discoloration, degeneration, and overcooking, and methods for treating food additives by processing food additives have been developed. It is required to remove the nitrosoamine, which is a carcinogenic substance caused by protein denaturation in meat processing, and to treat antioxidants to inhibit sourness and deterioration of fat. Therefore, there is an urgent need to develop a natural flavor enhancer which has excellent protein decomposition activity, has a meat effect, has an abundance of antioxidant physiologically active substances such as vitamins and polyphenols, and uses a herb medicine.
상기의 단백질 분해활성이 우수하고 항산화 생리활성이 뛰어난 것으로는 감, 키위, 배, 천마(산약) 등이 알려져 있다. 감은 떫은맛을 내는데 이는 수용성 탄닌에 의한 것이며, 탄닌 성분은 수렴성, 지혈작용, 향균, 항산화, 항종양작용 및 중금속 제거기능 등의 약리적 효과가 있다. 더불어 단백질이나 알칼로이드와 결합하는 특성을 지니고 있다. 또한, 키위에는 비타민 C의 함량이 높아 우수한 항산화활성이 있고 단백질 분해효소가 함유되어 있어 육류의 연육 효과도 기대된다. 그리고 통상적으로 고기의 연육을 목적으로 배를 이용하는데, 이는 배에 함유된 단백질 분해효소에 의한 것으로 보고되었다(Cheo et al., 1996). 그리고 상기 산약에는 가스트로딘(gastrodin), 페놀성 배당체, 유황 함유 페놀성 화합물, 유기산, 당 및 β-sitosterol 등의 성분이 보고되었으며, 그 이외에도 sterol류, cholesterol, p-hydroxylbenvyl alcohol과 vanillin 등의 성분에 대해서도 알려져 있다. 천마의 가스트로딘은 폴리페놀의 일종으로서 유해산소 제거능이 우수하다. 산약을 이용한 상업적 가공제품은 추출음료, 환, 타브렛 정도로 제품이고 항산화, 혈당강하, 항명증, 항암, 항비만, 지질대사 개선, 면역력 증가 등의 생리활성이 알려진 생약제로써 육류섭취로 유발될 수 있는 성인질환의 예방을 목적으로 식품첨가물로 이용하기에 적합하다.As for the above-mentioned proteolytic activity and excellent antioxidative physiological activity, persimmon, kiwi, pear, and chamaemyeo are known. It has a pharmacological effect such as astringent, hemostatic, antibacterial, antioxidant, antitumor action and removal of heavy metals. In addition, it has the property of binding to proteins and alkaloids. In addition, kiwi has high antioxidant activity because it has a high content of vitamin C, and it contains proteolytic enzymes, so that it is expected to have a meat effect. It is also commonly used to breed meat, which has been reported to be caused by proteolytic enzymes contained in the abdomen (Cheo et al., 1996). In addition, the above-mentioned ingredients include gastrodin, phenolic glycosides, sulfur-containing phenolic compounds, organic acids, sugars and β-sitosterol, and other ingredients such as sterols, cholesterol, p-hydroxylbenvyl alcohol and vanillin Are also known. Gastrodin is a kind of polyphenol which has excellent ability to remove harmful oxygen. Commercially processed products using acidic beverages are products of extract beverages, rings and tabrets, and are known to have physiological activities such as antioxidation, hypoglycemia, anemia, anticancer, anti-obesity, improvement of lipid metabolism and immunity. Which is suitable for use as a food additive for the purpose of preventing adult diseases.
상기와 같은 이유로 저작 및 소화력이 떨어지는 노인들의 단백질 먹을거리로서 육류를 제공하기 위하여 저작이 용이하고 소화에 도움을 줄 수 있을 뿐만 아니라 기능성이 부가된 연육제의 개발은 고령화 사회에 필수적인 제품이 되리라 사료된다.
For the above reasons, it is easy to chew and provide digestion to provide meats as a protein food for seniors whose chewing and digestion power is low, and the development of a functional food additive will become an indispensable product for an aging society. do.
연육제에 관한 선행기술 대한민국 공개특허 제10-2003-0035205호에서는 키위와 무화과를 이용한 생약제 추출물 함유 연육제 및 이의 제조방법에 관하여 개시하고 있으며, 대한민국 공개특허 제10-2010-0115481호에서는 배, 키위, 오디, 생강, 겨우살이 잎 및 줄기 분말들로 조성된 연육제를 개시하였으나 상기의 종래기술들은 단지 고기의 연화정도만을 평가한 것이다. 또 대한민국 공개특허 제10-2010-0082681호에서는 오매, 모과, 단풍을 침출하여 그 추출물을 혼합하여 구성한 연육제를 고기에 처리하고 상기 처리한 고기의 물성과 관능평가 결과를 개시하였다. 이상에서 살펴 본바 육류 요리중 생성되는 아질산염과 관련하여 이를 제거하기 위해 고안된 선행기술은 공지된바 없다.Prior Art Korean Patent Laid-Open No. 10-2003-0035205 discloses a concentrate containing an extract of a herb medicine using a kiwi and a fig and a preparation method thereof, and in Korean Patent Publication No. 10-2010-0115481, Kiwi, gum, ginger, mistletoe leaves and stem powders. However, the above-mentioned conventional techniques only evaluate the degree of softening of meat. Korean Patent Laid-Open No. 10-2010-0082681 also discloses the physical properties and sensory evaluation results of the meat treated with a frying agent prepared by leaching oats, quince and autumn leaves and mixing the extracts. As described above, there is no known prior art designed to remove the nitrite produced in the meat dish.
따라서 본 발명의 목적은 상기 문제점을 감안하여 육류의 연육 및 소화능을 향상시키는 생약제 추출물을 함유하는 연육제를 제공하는 데 있다. DISCLOSURE OF THE INVENTION Accordingly, it is an object of the present invention to provide a frying agent containing an extract of a herb medicine which improves the meat softening and digestibility of meat.
본 발명의 다른 목적은, 생약제를 이용하여 육류 중 단백질 분해, 항산화활성을 증대하고 요리 중 생성되는 아질산염을 제거하는 활성이 있는 연육제 분말을 제공하는 데 있다. Another object of the present invention is to provide a soft meat powder having an activity of increasing proteolytic activity and antioxidative activity in meats by using a herbal medicine and removing nitrite formed during cooking.
본 발명의 상기 목적은, 감, 키위, 배 및 산약을 각각 추출하는 단계와; 상기에서 얻은 4종의 추출물을 동결건조하여 분말화하는 단계와; 상기에서 얻은 4종의 분말을 조합하여 3종의 혼합물을 제조하는 단계와; 상기에서 얻은 3종의 혼합물의 항산화활성 및 아질산염 소거능력을 평가하는 단계와; 이와 별도로 상기에서 얻은 3종의 혼합물을 고기에 시간별로 처리하여 고기의 품질평가를 하는 단계와; 또 이와 별도로 상기 3종의 혼합물을 유효성분으로 함유하는 연육소스을 제조하는 단계와; 상기 연육소스를 시간별로 처리한 고기의 품질평가를 하는 단계를 통하여 달성하였다.The above object of the present invention can be achieved by a method of extracting persimmon, kiwifruit, Lyophilizing the four kinds of extracts obtained in the above-described manner and pulverizing them; Preparing a mixture of three kinds by combining the four kinds of powders obtained above; Evaluating the antioxidative activity and the nitrite scavenging ability of the three kinds of mixtures obtained above; Separately evaluating the quality of the meat by treating the mixture of the three species obtained above with time; Separately preparing a meat sauce sauce containing the above three kinds of mixtures as an active ingredient; And evaluating the quality of the meat treated with the meat sauce over time.
본 발명은 고기의 육질을 연육시킴으로서 기호성을 증대시키고 저작 및 소화능을 향상시키는 연육제 분말을 제공하는 효과가 있다. The present invention has the effect of providing a fleshy powder which enhances palatability and improves masticatory and digestibility by flesh meat.
또 본 발명은, 단백질 분해, 항산화 및 아질산염 제거 활성이 있는 생약제를 함유하는 연육소스를 제공하는 뛰어난 효과가 있다.Further, the present invention has an excellent effect of providing a meat source containing a herb medicine having proteolytic, antioxidant and nitrite-removing activity.
도 1은 본 발명 생약제 추출물 함유 연육제 분말 조성에 따른 각각의 전자소거능 활성에 관한 결과이다.
도 2는 본 발명 생약제 추출물 함유 연육제 분말 조성에 따른 각각의 SOD 유사활성에 관한 결과이다.
도 3은 본 발명 생약제 추출물 함유 연육제 분말 조성에 따른 pH 1.2 반응조건에서의 아질산염 제거활성에 관한 결과이다.
도 4는 본 발명 생약제 추출물 함유 연육제 분말 조성에 따른 pH 3.0 반응조건에서의 아질산염 제거활성에 관한 결과이다.
도 5는 본 발명 생약제 추출물 함유 연육제 분말 조성에 따른 pH 6.0 반응조건에서의 아질산염 제거활성에 관한 결과이다.
도 6은 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 저장기간별 pH 변화에 관한 결과이다.
도 7은 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 저장기간별 수분함량 변화에 관한 결과이다.
도 8은 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 저장기간별 미생물 총균수 변화에 관한 결과이다.
도 9는 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 명도(L)를 측정한 결과이다.
도 10은 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 적색도(a)를 측정한 결과이다.
도 11은 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 황색도(b)를 측정한 결과이다.
도 12는 본 발명 생약제 추출물 함유 연육제 분말을 처리한 고기의 관능평가 결과이다.
도 13은 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의저장기간별 pH 변화에 관한 결과이다.
도 14는 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의저장기간별 수분함량 변화에 관한 결과이다.
도 15는 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 저장기간별 미생물 총균수 변화에 관한 결과이다.
도 16은 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 명도(L)를 측정한 결과이다.
도 17은 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 적색도(a)를 측정한 결과이다.
도 18은 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 황색도(b)를 측정한 결과이다.
도 19는 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 관능평가 결과이다.
도 20은 본 발명 생약제 추출물 함유 연육제 분말을 유효성분으로 함유하는 연육소스를 처리한 고기의 외형을 나타낸 그림이다.FIG. 1 shows the results of the respective electron scavenging activity according to the composition of the soft meat powder containing the crude extract of the present invention.
FIG. 2 shows the results of SOD-like activities according to the composition of the soft granule powder containing the herbal medicine extract of the present invention.
FIG. 3 shows the nitrite scavenging activity at the pH 1.2 reaction condition according to the composition of the soft meat powder containing the crude extract of the present invention.
FIG. 4 shows the results of nitrite removal activity at pH 3.0 reaction conditions according to the composition of the soft granule powder containing the crude extract of the present invention.
FIG. 5 shows the nitrite scavenging activity at the pH 6.0 reaction condition according to the composition of the soft meat powder containing the herbal medicine extract of the present invention.
FIG. 6 shows the results of pH change of meat treated with the extract powder containing crude extract of the present invention for each storage period.
FIG. 7 is a graph showing changes in moisture content of meat treated with the extract powder containing crude extract of the present invention for each storage period.
8 is a graph showing changes in the total microbial counts of the meat treated with the crude extract powder containing the crude extract of the present invention by storage period.
Fig. 9 shows the results of measuring the lightness (L) of meat treated with the extract powder containing the herb extract of the present invention.
Fig. 10 shows the results of measuring the redness (a) of the meat treated with the extract powder containing the herbal medicine extract of the present invention.
Fig. 11 shows the results of measuring the yellowness (b) of the meat treated with the extract powder containing the herbal medicine extract of the present invention.
Fig. 12 shows the results of sensory evaluation of meat treated with the extract powder containing crude extract of the present invention.
Fig. 13 shows the results of pH change of meat treated with sauce containing safflower powder containing the crude extract powder of the present invention as an active ingredient.
Fig. 14 shows the results of moisture content changes of meat treated with sauce containing safflower powder containing the crude extract powder of the present invention as an active ingredient.
Fig. 15 shows the results of the change in microbial counts of the meat treated with the sauce containing the crude extract powder containing the crude extract powder of the present invention as an active ingredient by storage period.
Fig. 16 shows the results of measuring the lightness (L) of meat treated with a sauce containing an extract powder containing the crude extract of the present invention as an active ingredient.
Fig. 17 shows the results of measuring the redness (a) of meat treated with a sauces containing an extract powder containing the herbal medicine extract of the present invention as an active ingredient.
Fig. 18 shows the results of measurement of yellowness (b) of meat treated with a sauces containing an extract powder containing the crude extract of the present invention as an active ingredient.
Fig. 19 shows the results of sensory evaluation of meat treated with a sauces containing an extract powder containing the crude extract of the present invention as an active ingredient.
FIG. 20 is a diagram showing the appearance of meat treated with a sauces containing an extract powder containing the crude extract of the present invention as an active ingredient. FIG.
<< 실시예Example 1> 본 발명 생약제 추출물 함유 연육제 분말제조 1> Production of granular powder containing extract of herbal medicine
본 발명에서 사용한 감(청도), 키위(영암), 배(안성), 산약(안동, 건조분말)을 구입하여 본 발명의 공시재료로 사용하였다. 상기 감, 키위, 배는 껍질 등을 손질한 후 0.2 cm 정도로 얇게 썰어서 50℃ 건조기에서 30시간 동안 건조하여 조분쇄 하였다. 상기 분쇄한 4종의 조분쇄물 20 g에 증류수 100 mL를 가하여 85℃에서 3시간 동안 2회 추출하였다. 상기 4종의 추출물은 Whatman No. 1로 여과한 후 회전식증발농축기(Tokyo, Rikakikai Co., Japan)로 농축하고 그 농축물을 동결건조기(SFDSF24, Sanwon, Korea)로 동결건조하여 분말화 하여 본 발명의 공시시료로 사용하였다. (Qingdao), kiwi (young rock), pear (Anseong), and liquor (Andong, dry powder) used in the present invention were purchased and used as the disclosure material of the present invention. The persimmon, kiwi, and pear were cut into thin pieces of about 0.2 cm, dried at 50 ° C for 30 hours, and then pulverized. 100 g of distilled water was added to 20 g of the coarse pulverized product, and the mixture was extracted twice at 85 캜 for 3 hours. The extracts of the four species were identified as Whatman No. 1, concentrated using a rotary evaporator (Tokyo, Rikakikai Co., Japan), and the concentrate was lyophilized with a freeze dryer (SFDSF24, Sanwon, Korea) and powdered to be used as a test sample of the present invention.
상기 4종의 추출분말의 바람직한 배합비를 결정하기 위하여 하기 표 1과 같이 생약제 추출물 함유 연육제 분말을 제조하였다.In order to determine a preferable blending ratio of the above four kinds of extracted powders, a crude powder containing crude drug extract was prepared as shown in Table 1 below.
< < 실시예Example 2> 본 발명 생약제 추출물 함유 연육제 분말의 항산화 및 2> Antioxidative and antioxidative activities of the extract powder containing the herb extract 나이트로젠Nitrogene 소거 활성 검정 Scavenging activity test
항산화능 검증 실험에 사용된 시약인 1-1- diphenyl-2-picryl hydeazyl (DPPH), Pyrogallol, Trichloroacetic acid (TCA) Griess reagent (sulfanilic acid, naphthlamine) 등은 Sigma Chemical Co. (St. Louis, MO., U.S.A)에서 구입하였고, 2-thiobarbituric acid (TBA)는 Eastern Organic Chemical (Roochester, NY.)에서 구입하여 사용하였다. 실험에 사용된 기기는 UV/vis spectrophotometer (Hitachi, Japan), Rotaaary vacuum evaporator (Tokyo, Rikakikai Co., Japan), Centrifuge (Hitachi, Japan), CO2 Incubator (Hanbaek Scientific Co., Korea), pH meter (HM-20S, TOA, Japan), Rheometer (Sun Sci. compac 100Ⅱ), Color meter (Minolta, CR-300, Japan), Autoclave (SH-AC60, Korea) 등을 사용하여 측정하였다.
1-diphenyl-2-picryl hydeazyl (DPPH), Pyrogallol, Trichloroacetic acid (TCA) Griess reagent (sulfanilic acid, naphthlamine) (St. Louis, Mo., USA) and 2-thiobarbituric acid (TBA) was purchased from Eastern Organic Chemical (Roochester, N.Y.). The instrument used was a UV / vis spectrophotometer (Hitachi, Japan), a rotary vacuum evaporator (Tokyo, Rikakikai Co., Japan), Centrifuge (Hitachi, Japan), CO2 Incubator (Hanbaek Scientific Co., HM-20S, TOA, Japan), Rheometer (
실험예Experimental Example 2-1. 본 발명 생약제 추출물 함유 연육제 분말의 2-1. In the case of the crude extract powder containing the crude extract of the present invention 전자공여능Electron donating ability
전자공여능 측정은 DPPH 라디칼 소거법으로 측정하였으며, DPPH는 비교적 안정한 라디칼을 갖는 물질로 항산화 활성이 있는 물질과 만나면 라디칼이 소거되어 탈색되는 점을 이용하여 항산화 활성을 검정한다. The electron donating ability was measured by the DPPH radical scavenging method. DPPH is a substance having a relatively stable radical, and antioxidant activity is assayed using a point where radicals are eliminated and decolorized when a substance having antioxidative activity is encountered.
전자공여능(EDA: electron donating ability)은 상기 실시예 1에서 제조한 3종의 본 발명 생약제 추출물 함유 연육제 분말 시료를 100, 300, 500 ppm 농도로 제조하였다. 그 시료 2 mL에 2×10-4 M DPPH 1.0 mL를 넣고 vortex한 후 30분 동안 방치한 다음 517 nm에서 흡광도를 측정하였다. 전자공여능은 [1-(시료첨가구의 흡광도-시료의 흡광도/시료 무첨가구의 흡광도)]×100으로 나타내었다.
The electron donating ability (EDA) of each of the three crude extract powder preparations of the present invention prepared in Example 1 was 100, 300 and 500 ppm. 1.0 mL of 2 × 10 -4 M DPPH was added to 2 mL of the sample, vortexed, left for 30 minutes, and then absorbance was measured at 517 nm. The electron donating ability is represented by [1- (absorbance of sample addition sphere - absorbance of sample / absorbance of sample not added sphere)] x 100.
본 발명 생약제 추출물 함유 연육제 분말 3종(제조예 1 내지 제조예 3)의 전자소거능을 측정한 결과는 도 1과 같이 제조예 3 > 제조예 2 > 제조예 1의 순서로 나타났다. 제조예1 내지 제조예 3을 각각 농도별로 처리하였을 때 그 처리 농도가 증가함에 따라 유의적으로 높은 전자공여능을 나타냈다. 또 감 추출분말 단독으로 처리한 전자소거능 결과보다 감 추출분말과 키위 추출분말을 1:1로 혼합한 분말의 전자공여능이 더 높았다. 특히, 감, 키위, 배 및 산약 추출분말을 각각 2:3:3:2 비율로 혼합하여 제조(제조예 3) 했을때 전자공여능이 가장 우수하게 나타났다. 전자공여능 작용은 활성 라디칼에 전자를 공여하여 식품 중에 지방질 산화를 억제하는 목적으로 사용되고 있을 뿐만 아니라, 인체 내에서 활성라디칼에 의한 노화를 억제하는 작용의 목적으로 이용되고 있다. 따라서 본 발명 생약제 추출물 함유 연육제 분말을 고기 가공 및 요리에 사용하면 조리시 발생하는 산화물질의 제거가 용이하다.
The electron scavenging ability of the three kinds of soft powdery powders containing the crude extract of the present invention (Production Examples 1 to 3) was measured in the order of Production Example 3> Production Example 2> Production Example 1 as shown in FIG. When
실험예Experimental Example 2-2. 본 발명 생약제 추출물 함유 연육제 분말의 2-2. In the case of the crude extract powder containing the crude extract of the present invention SODSOD 유사활성Similar activity 검정 black
항산화 효소 중의 하나인 superoxide dismutase(SOD)는 세포에 유해한 환원 산소종을 과산화수소로 전환 시키는 반응(2O2- + 2H+ → H2O2 +O2)을 촉매하는 효소이며, SOD에 의해 생성된 H2O2는 peroxidase나 catalase에 의하여 무해한 물 분자와 산소분자로 전화시켜 산소 상해로부터 생체를 보호하는 기능으로 알려져 있다. SOD 유사활성 물질은 효소는 아니지만 SOD와 유사한 역할을 하는 저분자 물질로 주로 phytochemicals에 속하며 superoxide의 반응성을 억제하여 산화적 장애를 방어할 수 있다. One of the antioxidant enzymes, superoxide dismutase (SOD), is an enzyme that catalyzes a reaction (2O 2 - + 2H + → H 2 O 2 + O 2 ) that converts harmful reducing oxygen species to hydrogen peroxide 2 O 2 is known to be a function to protect living organisms from oxygen injury by dialing harmless water molecules and oxygen molecules by peroxidase or catalase. The SOD-like active substance is not an enzyme but is a low molecular substance similar to SOD, mainly belonging to phytochemicals, and can inhibit the oxidative damage by suppressing the reactivity of superoxide.
SOD 유사활성 측정은 상기 실시예 1에서 제조한 3종의 본 발명 생약제 추출물 함유 연육제 분말 시료를 100, 300, 500 ppm 농도로 제조하였다. 상기 각 시료 0.2 mL에 pH 8.5로 보정한 tris-HCl buffer(50 mM tris[hydroxymethyl] aminomethane + 10 mM EDTA) 3 mL와 7.2 mM pyrogallol 0.2 mL를 가하고 25℃에서 10분간 방치한 후 1 N HCl 1 mL로 반응을 정지시킨 후 420 nm에서 흡광도를 측정하여 100-[(시료참가구의 흡광도/무첨가구의 흡광도)×100]으로 나타내었다.
SOD-like activities were measured at the concentrations of 100, 300 and 500 ppm of the three crude powdery extracts containing the herb extracts of the present invention prepared in Example 1 above. To each 0.2 mL of each sample, add 3 mL of tris-HCl buffer (50 mM tris [hydroxymethyl] aminomethane + 10 mM EDTA) and 0.2 mL of 7.2 mM pyrogallol adjusted to pH 8.5 and incubate at 25 ° C for 10 minutes. Add 1 N HCl mL, and the absorbance at 420 nm was measured to be 100 - [absorbance of the sample boiled / absorbance of the untreated sample] × 100].
SOD 유사활성을 측정한 결과는 도 1과 같이 제조예 3 > 제조예 2 > 제조예 1의 순서로 나타났다. 제조예1 내지 제조예 3을 각각 농도별로 처리하였을 때 그 처리 농도가 증가함에 따라 유의적으로 높은 SOD 유사활성을 나타냈다. 또 감 추출분말 단독으로 처리한 SOD 유사활성보다 감 추출분말과 키위 추출분말을 1:1로 혼합한 분말의 SOD 유사활성이 더 높았다. 특히, 감, 키위, 배 및 산약 추출분말을 각각 2:3:3:2 비율로 혼합하여 제조(제조예 3) 했을때 SOD 유사활성이 가장 우수하게 나타났다. 추출물의 농도가 증가함에 따라 유의적으로 높은 SOD 유사활성을 나타내었다. 즉 제조예 1은 500 ppm에서 36.31%를 나타내었지만 제조예 3은 500 ppm 에서 60.34%로 가장 높은 SOD 유사활성을 나타냈다.
SOD-like activity was measured in the order of Production Example 3> Production Example 2> Production Example 1 as shown in FIG. As a result, the SOD-like activity was significantly increased as the treatment concentration increased. In addition, SOD - like activity was higher in powder mixture of sensory and kiwifruit extract powder than SOD - like extract powder alone. In particular, SOD-like activity was highest when the extract was prepared by mixing 2: 3: 3: 2 ratios of persimmon, kiwi, pear and acid extract powder (Preparation Example 3). As the concentration of extract increased, SOD - like activity was significantly higher. That is, while Production Example 1 showed 36.31% at 500 ppm, Production Example 3 showed the highest SOD-like activity at 500 ppm to 60.34%.
실험예Experimental Example 2-3. 본 발명 생약제 추출물 함유 연육제 분말의 아질산염 제거활성 검정 2-3. Determination of Nitrite Removal Activity of Soft Drink Powder Containing Extract of Herbal Drug
식품 중에 존재하는 아질산염은 그 자체로도 독성이 강하지만 특히 2급 아민과 함께 반응하여 강력한 발암물질인 nitrosoamine을 생성하는 것으로 알려져 있다. Nitrite present in food is toxic by itself, but it is known to react with secondary amines to produce nitrosoamine, a potent carcinogen.
아질산염 소거작용 측정은 1 mM NaNO2용액 2 mL에 상기 실시예 1에서 제조한 3종의 본 발명 생약제 추출물 함유 연육제 분말 시료를 100, 300, 500 ppm 농도로 1 mL을 첨가하여 혼합물을 제조하였다. 상기 혼합물을 0.1 N HCl(pH 1.2), 0.2 M 구연산 완충액(pH 3.0, pH 6.0)으로서 각각 pH 1.2, 3.0, 6.0으로 보정한 다음 반응용액의 부피를 10 mL로 하였다. 상기 반응 용액을 37℃에서 1시간 반응시킨 후 각 반응액을 1mL씩 취하여 2% 초산용액 2 mL와 30% 초산용액으로 반응을 종결하였다. 상기 반응 종결액에 Griess reagent(1% sulfanilic acid : 1% naphthylamine = 1 : 1) 0.4 mL를 가한 후 혼합하여 실온에서 15분간 방치 후 520 nm에서 흡광도를 측정하였다. 대조구는 Griess reagent 대신 증류수를 가하여 측정하였다.Nitrite scavenging activity was measured by adding 1 mL of each of the three crude powdery extracts of the present invention prepared in Example 1 at concentrations of 100, 300 and 500 ppm to 2 mL of 1
본 발명 제조예 1 내지 3의 처리 농도에 따라 pH 1.2, pH 3.0 및 pH 6.0에서 반응시켜 아질산염을 조사한 결과는 도 3 내지 도 5에 나타내었다. 상기 아질산염 제거 반응액의 pH를 1.2, 3.0, 6.0으로 각각 보정하여 반응하였을 경우 제조예 1내지 제조예 3 각각의 농도가 증가할수록 유의적으로 높은 아질산염 소거능을 나타내어 농도 의존적인 활성을 보였다. 특히 상기 반응액의 pH가 6.0일 때 제조예 3은 62.74%로 가장 좋은 활성을 나타냈다.
The results of nitrite irradiation at pH 1.2, pH 3.0 and pH 6.0 according to the treatment concentrations of Production Examples 1 to 3 of the present invention are shown in FIG. 3 to FIG. When the pH of the nitrite removal reaction solution was adjusted to 1.2, 3.0, and 6.0, respectively, the nitrite scavenging ability was significantly increased as the concentration of each of Preparation Examples 1 to 3 was increased, indicating a concentration-dependent activity. Particularly, when the pH of the reaction solution was 6.0, Production Example 3 showed the best activity as 62.74%.
따라서, 본 발명 생약제 추출물 함유 연육제는 상기 표 1의 제조예 3과 같이 제조되었을 때 가장 좋은 항산화 활성과 아질산염제거 활성을 나타냈다.
Therefore, the concentrate containing the crude extract of the present invention exhibited the best antioxidative activity and nitrite eliminative activity when prepared as in Preparation Example 3 of Table 1 above.
<< 실시예Example 3> 본 발명 생약제 추출물 함유 연육제 분말을 처리한 육질의 품질평가 3> Quality Evaluation of Meat Quality Treated with Crude Powder Containing the Extract of Herbal Drug
상기 표 1에 기재한 제조예 1 내지 제조예 3을 고기표면에 각각 15 g의 양으로 처리한 후 시간별로 육질의 변화를 조사하였다. After the meat surface was treated with 15 g of each of Production Examples 1 to 3 described in Table 1, the change in meat quality was examined over time.
실험예Experimental Example 3-1. 3-1. pHpH 측정 Measure
상기 본 발명 생약제 추출물 함유 연육제 분말 제조예 1 내지 제조예 3이 처리된 고기의 pH 측정은 A.O.A.C법에 준하여 측정하였다. 즉, 일반적인 방법에 따라 상기 본 발명 생약제 추출물 함유 연육제 분말이 처리된 3종의 고기 10 g을 증류수 90 mL를 넣고 homogenizer로 균질화한 후 여과한 여과액을 시험용액으로 하였다. 상기 시험용액은 pH meter(Model DP-135M)를 사용하여 측정하였다.
The pH of the meat treated with the crude powdery powders Production Examples 1 to 3 containing the crude herbal medicine extract according to the present invention was measured according to the AOAC method. That is, 90 g of distilled water was added to 10 g of the three kinds of meat treated with the granule-containing powder of the herbal extract according to the general method, homogenized with a homogenizer, and the filtered solution was used as a test solution. The test solution was measured using a pH meter (Model DP-135M).
상기 본 발명 생약제 추출물 함유 연육제 분말 처리 고기는 저장기간별(7일)로 pH를 측정하여 결과는 도 6에 나타냈다. 제조예 1은 저장 초기에는 pH 6.14이었으나 7일이 경과 후에는 pH 5.84로 조금 감소하는 경향이 있었다. 또 제조예 3을 처리한 고기는 저장 초기에는 pH 6.10이었으나 7일 경과 후에는 pH 5.70으로 감소하였다. 저장기간에 따른 pH 감소의 원인은 육류로부터 CO2 발생에서 기인한 듯하다.
The pH of the meat-treated meat containing the herbal extract of the present invention was measured by storage period (7 days). The results are shown in FIG. In Preparation Example 1, the pH was 6.14 at the initial stage of storage, but tended to decrease slightly to pH 5.84 after 7 days. The meat treated with Preparation Example 3 was pH 6.10 at the initial stage of storage but decreased to pH 5.70 after 7 days. The reason for pH decrease over storage period seems to be caused by CO 2 generation from meat.
실험예Experimental Example 3-2. 수분 정량 3-2. Moisture determination
수분(moisture)정량은 본 발명 생약제 추출물 함유 연육제 분말이 각각 처리된 고기를 시간별로 취하여 상압건조법에 따라 측정하였다. 즉, 칭량병의 항량을 먼저 구한 후 시료를 넣고, 105℃의 열건조기에서 2~3시간 가열 후 desiccator에서 30분간 방냉한 후 칭량병의 무게를 측정하여 항량이 될 때까지 반복 측정하였다.
Moisture content was determined according to the atmospheric pressure drying method by taking the meat treated with the granular powder containing the crude extract of the present invention with time. That is, the weight of the weighing bottle was firstly measured, the sample was added, the sample was heated in a heat drier at 105 ° C. for 2 to 3 hours and then left to stand for 30 minutes in a desiccator. The weight of the weighing bottle was measured repeatedly until it became constant.
도 7에 나타난 바와 같이 전반적으로 수분량은 제조예 1 내지 제조예 3을 처리함에 의해서 높게 나타났으며 저장기간이 경과 하여도 고기의 수분량에는 큰 변화는 없었다. 그러나 제조예 3을 처리한 고기에서는 수분함량이 조금 높게 나타난 경향이었으나 조직감에 큰 영향을 미치지는 못한 것으로 사료된다.As shown in FIG. 7, the overall moisture content was high in the case of Preparation Examples 1 to 3, and there was no significant change in the moisture content of the meat even after the storage period. However, the moisture content of the meat treated with Preparation Example 3 tended to be slightly higher, but it did not affect the texture significantly.
실험예Experimental Example 3-3. 3-3. 총균수Total number of bacteria 측정 Measure
본 발명 생약제 추출물 함유 연육제 분말이 각각 처리된 고기 10 g을 90 mL의 희석수와 함께 균질화한 후 각각의 균질액에 0.1% 멸균 펩톤용액을 사용하여 적정 희석하였다. 상기 희석액 0.1 mL를 TSA배지에 접종하여 35℃에서 48시간 배양하여 생균수를 측정하였다. 생균수의 변화는 3회 반복하여 실험한 값을 평균하였으며, colony forming unit(CFU/g)로 나타내었다.
10 g of the meat treated with the crude extract powder containing the crude extract powder of the present invention was homogenized together with 90 mL of diluted water and appropriately diluted with each of the homogenates using a 0.1% sterilized peptone solution. 0.1 mL of the diluted solution was inoculated into TSA medium and cultured at 35 DEG C for 48 hours to measure viable cell count. Changes in viable counts were averaged three times and the results were expressed as colony forming unit (CFU / g).
상기 표 1의 제조예 1 내지 3을 각각 고기에 처리한 후 저장기간에 따른 총균수 측정결과는 도 8에 나타냈다. 저장기간이 경과함에 따라 총균수는 증가하였다. 저장 초기에는 총 균수가 제조예 1 > 제조예 2 > 제조예 3의 순서로 3.1×103 cfu/g, 3.3×103 cfu/g, 3.3×103 cfu/g이였으나, 저장 7일째에는 총 균수가 제조예 1 > 제조예 2 > 제조예 3의 순서로 4.8×103 cfu/g, 4.4×103 cfu/g, 4.2×103 cfu/g로 나타났다. 따라서 상기 제조예 3의 처리에 의해서 미생물의 생장이 가장 뛰어나게 억제되어 가장 적은 총균수를 나타내었다. 상기 결과로서 제조예 3이 항산화 및 아질산염 제거활성과 더불어 미생물 생장 억제 효과도 우수함을 알 수 있었다.
The results of the measurement of the total number of bacteria according to the storage period after treating the meat of each of Production Examples 1 to 3 in Table 1 are shown in FIG. Total bacterial counts increased with storage period. At the initial stage of storage, the total number of bacteria was 3.1 × 10 3 cfu / g, 3.3 × 10 3 cfu / g and 3.3 × 10 3 cfu / g in the order of Production Example 1> Production Example 2> Production Example 3, 4.8 × 10 3 cfu / g, 4.4 × 10 3 cfu / g and 4.2 × 10 3 cfu / g in the order of Production Example 1> Production Example 2> Therefore, the growth of microorganisms was most effectively inhibited by the treatment of Preparation Example 3 and the smallest total number of bacteria was obtained. As a result, it was found that Production Example 3 has excellent antioxidative and nitrite removal activity as well as microorganism growth inhibition effect.
실험예Experimental Example 3-4. 3-4. 색차Color difference
본 발명 생약제 추출물 함유 연육제 처리 고기의 색차 측정은 Color Difference Meter(CM-3600d, Minolta, Japan)을 이용하여 측정하였다. 측정시료는 공기 중 30분간 발색 시킨 후 Hunter값 즉, 명도(L : lightness), 적색도(a : redness), 황색도(b : yellowness)값으로 나타내었으며, 시료당 3회 반복하여 그 평균값을 나타내었다.
The color difference measurement of the processed meat containing the crude extract of the present invention was measured using a Color Difference Meter (CM-3600d, Minolta, Japan). The measured values were expressed as Hunter values (L: lightness), redness (a: redness), and yellowness (b) values after coloring for 30 minutes in air. Respectively.
고기의 신선도의 척도로 이용이 되는 육색은 고기의 품질평가에 있어서 중요한 부분이다. 육색은 육의 상태와 등급의 판단기준에 속하기 때문에 육색의 유지는 신선도 유지로도 여겨진다. 저장기간에 따라 명도의 변화는 도 9에 나타냈다. 명도는 제조예 2에서 가장 높게 나타났으며, 적색도는 도 10에 나타냈으며 전반적으로 제조예 1에서 가장 높게 나타났으나 유의적인 차이는 나타나지 않았다. 황색도는 도 11에 나타낸 바와 같이 제조예 1에서 가장높게 나타났으나 저장기간에 따른 유의적인 차이는 나타나지 않았다.
The meat color which is used as a measure of freshness of meat is an important part in the quality evaluation of meat. The color of meat is regarded as a criterion for judging the condition and grade of meat. The change in brightness according to the storage period is shown in Fig. The lightness was highest in Production Example 2, and the redness was shown in Fig. 10, which was the highest in Production Example 1 as a whole, but no significant difference was observed. As shown in Fig. 11, yellowness was highest in Production Example 1, but no significant difference was observed during storage period.
실험예Experimental Example 3-5. 물성측정 3-5. Property measurement
물성측정은 Rheometer(COMPAC-100Ⅱ, SUN SCIENTIFICCO., LTD)를 사용하여 5회 이상 측정하여 그 평균치를 사용하였다. 상기 표 1의 제조예 1내지 3을 고기에 각각 처리한 후 저장 기간별로 각각의 고기를 2×2×1 cm로 절단하여 강도, 연도, 탄력성, 응집성, 씹음성 5가지 항목을 측정하였다. 상기 절단한 각각의 고기에 직경 15 mm 원형통 plunger를 부착시켜 최대하중 2 Kg, table speed 60mm/min, 압착률 50%에서 측정하였다.
The physical properties were measured five times or more using a Rheometer (COMPAC-100Ⅱ, SUN SCIENTIFICCO., LTD) and the average value was used. Each of the meat was cut into 2 × 2 × 1 cm for each storage period after preparing the meat of each of the preparation examples 1 to 3 of Table 1, and then five items such as strength, year, elasticity, cohesiveness and chewiness were measured. Each cut meat was attached to a 15 mm diameter circular plunger and measured at maximum load of 2 Kg, table speed of 60 mm / min, and compression ratio of 50%.
물성 측정 결과는 하기 표 2에 나타냈다. 강도는 저장기간이 경과함에 따라 조금씩 증가하는 경향을 보였으나 제조예 3이 가장 낮게 나타났다. 연도는 저장기간이 경과함에 유의적인 차이는 보이지 않았고 탄력성과 응집성 그리고 씹음성은 저장기간이 경과함에 따라 유의적으로 감소하는 경향을 보였다. 육의 연육정도를 비교하였을 때는 제조예 3의 수치가 가장 낮게 측정되어 고기의 육질의 연화과 진행된 것으로 사료된다. The results of physical property measurement are shown in Table 2 below. The strength tended to increase little by little as the storage period elapsed, while the lowest value was found in Manufacturing Example 3. There was no significant difference in the storage period of the year, and the elasticity, cohesiveness and chewiness tended to decrease significantly with storage period. When the degree of meat texture was compared, the value of Production Example 3 was measured to be the lowest, and it was considered that the meat was softened and the meat was softened.
실험예Experimental Example 3-6. 관능검사 3-6. Sensory test
관능검사는 시료의 물리적, 화학적 및 미생물학적 변화에 따라서 영향을 받는 것으로서 본 관능검사를 통하여 본 발명 제조예들의 기호성뿐만 아니라 제품평가도 동시에 확인 가능하다.The sensory test is influenced by the physical, chemical and microbiological changes of the sample. Through the sensory test, not only the palatability but also the product evaluation of the present invention can be confirmed at the same time.
관능검사는 색, 향, 맛, 그리고 조직감으로 평가하였다. 평가방법은 5점 검사법으로 실시하였으며 선정된 관능요원은 충분한 훈련을 거쳐 품질차이를 식별할 수 있는 능력을 갖추었다고 여기지는 6명으로 구성되었다. 관능검사 항목에 대해 매우 나쁘다 1점, 나쁘다 2점, 보통이다 3점, 좋다 4점, 매우 좋다 5점으로 각 시료를 평가하였다.
Sensory evaluation was evaluated by color, flavor, taste and texture. The evaluation method was carried out by the 5 - point test method, and the selected sensory agents were composed of 6 persons who were assumed to have the ability to identify the quality difference through sufficient training. For each sensory test item, each sample was evaluated with a score of 1, 2, 3, 4, and 5, which is very bad.
상기 표 1의 제조예 1 내지 제조예 2를 각각 고기에 뿌린 후 저장기간에 따른 관능검사 결과는 도 12에 나타냈다. 색, 향, 조직감 그리고 맛에 대한 기호도는 제조예3 > 제조예 2 > 제조예 1의 순서로 나타났다. The results of sensory evaluation according to the storage period after sprinkling on each of the preparation examples 1 to 2 of Table 1 are shown in FIG. The color, flavor, texture and taste were preferred in the order of Preparation Example 3> Production Example 2> Production Example 1.
상기 모든 실험예들의 결과에서 항산화 및 아질산염 제거 활성이 우수하였고 또 고기의 연화효과가 뛰어난 것으로 나타난 제조예 3이 기호성 면에서도 가장 좋은 결과를 나타냈다.
From the results of all the above examples, Production Example 3, which showed excellent antioxidative and nitrite removal activity and excellent softening effect of meat, showed the best results in terms of palatability.
<< 실시예Example 4> 본 발명 생약제 추출물 함유 연육제 분말을 함유하는 소스제조 4> Production of Sources Containing Granular Powder Containing the Herbal Drug Extract of the Invention
표 3에 기재한 제조예 4 내지 제조예 6을 고기표면에 각각 40 g의 양으로 처리한 후 시간별로 육질의 변화를 조사하였다. Production Examples 4 to 6 shown in Table 3 were treated with 40 g each on the meat surface, and the change in meat quality was examined over time.
본 발명 생약제 추출물 함유 연육제 분말을 함유한 소스의 효과는 상기 실험예 3-1 내지 3-6의 방법과 동일한 방법으로 실험을 수행하였다.
The effect of the sauce containing the extract powder containing the herbal medicine extract of the present invention was tested in the same manner as the methods of Experimental Examples 3-1 to 3-6.
실험예Experimental Example 4-1. 4-1. pHpH 측정 Measure
상기 표 3의 제조예 4 내지 제조예 6의 소스를 각각 고기에 양념한 후 저장기간 7일 동안 pH의 변화를 측정하였다. 그 결과 도 13에 나타난 바와 같이 저장초기와 저장 7일 이후의 pH의 변화가 거의 일어나지 않음을 알 수 있다. 이는 고기의 저장기간이 짧음에 따라 젖산이나 미생물의 번식이 거의 나타나지 않은 것으로 사료된다. Samples of Preparation Examples 4 to 6 of Table 3 were seasoned with meat, respectively, and then pH changes were measured for 7 days in storage period. As a result, as shown in FIG. 13, it can be seen that the pH changes little at the initial stage of storage and after 7 days of storage. This suggests that lactic acid or microbial propagation hardly occurs as the meat storage period is shortened.
실험예Experimental Example 4-2. 수분 정량 4-2. Moisture determination
저장기간에 따른 수분함량의 변화는 도 14에 나타난 바와 같이 상기 제조예 4 내지 제조예 6을 각각 처리한 고기의 저장기간이 경과함에 따라 수분 함량이 감소하는 경향이 있었으며 제조예 4(sample 1)의 수분함량이 약간 높은 경향을 보였고 제조예 5(sample 2)와 제조예 6(sample 3)은 거의 비슷한 경향을 보였다.
As shown in FIG. 14, the moisture content tended to decrease with the lapse of the storage period of the meat treated with each of the preparation examples 4 to 6, Showed a tendency to be slightly higher than that of Preparation Example 5, and the results of Preparation 5 (sample 2) and Preparation Example 6 (sample 3) were almost the same.
실험예Experimental Example 4-3. 4-3. 총균수Total number of bacteria 측정 Measure
저장기간 동안 총균수의 변화는 도 15와 같다. 상기 제조예 4 내지 제조예 6을 각각 처리한 고기에서 미생물 총 균수는 저장초기에 상기 3종의 제조예를 처리한 각각의 시료 모두에서 3.1×103 cfu/g으로 나타났다. 이후 저장 7일이 경과한 후에는 제조예 1 > 제조예 2 > 제조예 3의 순서로 미생물 총균수가 각각 4.3×103 cfu/g, 4.3×103 cfu/g, 4.1×103 cfu/g으로 측정되었다. 상기 결과에서 알 수 있듯이 균수의 증가는 크게 나타나지 않았고.
The change in total bacterial counts during the storage period is shown in FIG. The total number of microorganisms in the meat treated with each of the above Preparation Examples 4 to 6 was 3.1 × 10 3 cfu / g in all of the samples treated with the above three preparation examples at the initial stage of storage. After 7 days of storage, the total number of microorganisms was measured as 4.3 x 103 cfu / g, 4.3 x 103 cfu / g and 4.1 x 103 cfu / g, respectively, in the order of Production Example 1> Production Example 2> . As can be seen from the above results, the increase in the number of bacteria was not significant.
실험예 4-4. 색차EXPERIMENTAL EXAMPLE 4-4. Color difference
저장기간별 색도를 측정한 결과는 도 16 , 도 17, 도 18과 같다. 색차의 변화는 제조예 각각을 처리하여도 유의성 있는 차이는 나타나지 않았다. 명도(L)값은 저장기간이 길어질수록 감소하였고 이는 양념소스가 고기에 침투하여 명도(L)값이 낮아진 것으로 판단된다. 그러나 제조예 4를 처리한 고기보다 제조예 6을 처리한 고기의 명도가 조금 더 낮게 나타났다. 적색도(a)값은 저장기간이 지나면서 조금씩 감소하는 경향을 보였으나 제조예(4 내지 6) 처리에 따른 유의적인 차이는 나타나지 않았다. 황색도(b) 값 역시도 제조예(4 내지 6) 및 저장기간에 따른 큰 차이는 보이지는 않았다.
The results of measuring the chromaticity for each storage period are shown in FIG. 16, FIG. 17, and FIG. The difference in color difference was not significantly different even when each of the production examples was processed. The value of lightness (L) decreased as the storage period was longer. It is considered that the lightness (L) value of the sauce penetrated into the meat. However, the lightness of the meat treated with Preparation Example 6 was slightly lower than that of Preparation Example 4. The redness (a) value tended to decrease little by little over the storage period, but there was no significant difference according to the manufacturing processes (4-6). The value of yellowness (b) also did not show a large difference according to the production examples (4 to 6) and the storage period.
실험예Experimental Example 4-5. 물성측정 4-5. Property measurement
상기 제조예 4 내지 6 처리에 의한 고기의 저장기간에 따른 물성 측정 결과는 하기 표 4와 같다. 모든 처리구에서 강도나 연도는 저장기간이 경과함에 따라 조금씩 증가하는 경향을 보였다. 본 실험예 4-5에서 강도와 연도는 제조예 4 > 제조예 5 > 제조예 6의 순서로 낮게 나타났다. 또, 탄력성과 응집성은 저장기간이 경과함에 따라서 조금씩 감소하였으나 유의적인 차이는 보이지 않았다. 씹음성은 제조예 4 > 제조예 5 > 제조예 6 순으로 나타나 제조예 6이 가장 낮게 나타났다. 이로써 제조예 6을 처리한 고기의 육질이 가장 연하게 나타났고,
The results of measurement of physical properties according to the storage time of meat according to the production examples 4 to 6 are shown in Table 4 below. In all treatments, the intensity and the year tended to increase gradually with the passage of storage period. In the Experimental Example 4-5, the strength and the year were lower in the order of Production Example 4> Production Example 5> Production Example 6. In addition, the elasticity and cohesiveness decreased little by little as the storage time elapsed, but there was no significant difference. The chewiness was the lowest in the order of Production Example 4> Production Example 5> Production Example 6 in that order. As a result, the meat quality of the meat treated with Preparation Example 6 was the softest,
실험예Experimental Example 4-6. 관능검사 4-6. Sensory test
저장기간에 따른 관능검사 결과는 도 19와 같이 나타났다. 색, 맛 및 조직감에서는 제조예 4(sample 3)의 결과가 높게 나타났고 향은 모든 제조예 처리구에서 비슷한 결과를 보였으며, 저장기간이 경과함에 따라서 유의적으로 감소하는 경향이 나타났다.
The results of sensory evaluation according to the storage period were as shown in Fig. In the color, taste and texture, the results of Preparation Example 4 (sample 3) were high, and the incense was similar in all the preparation treatments, and showed a tendency to decrease with storage period.
최종적으로, 상기 제조예 4 내지 6을 처리한 고기는 도 20에 처리시간별로 정리하였고 상기 처리한 각각의 고기의 품질은 감, 키위, 배 및 산약 추출물 분말이 2:3:3:2의 비율로 배합된 혼합물로 제조된 분말 및 양념소스가 고기의 연육 및 항산화 효과 면에서 아주 뛰어남고 동시에 기호성 면에서도 우수한 것을 알 수 있었다. Finally, the meat processed in the above Production Examples 4 to 6 was sorted by the treatment time in FIG. 20, and the quality of each of the processed meat was evaluated as a ratio of 2: 3: 3: 2 in the sensory, kiwi, And safflower sauce were superior in terms of palatability and antioxidant effect of meat and excellent in palatability.
이상 설명한 바와 같이, 본 발명은 고기의 육질을 연육 시킴으로서 저작 및 소화능을 향상시키는 연육제를 제공하는 효과와 항산화 및 아질산염 제거 활성이 있는 생약제를 이용하여 육류가공 및 요리상에서 생성되는 산화물과 아질산염을 제거하는 연육소스를 제공하는 뛰어난 효과가 있으므로 식품가공 산업상 매우 유용한 발명이다.
INDUSTRIAL APPLICABILITY As described above, the present invention relates to a method for producing meat and meat products, which comprises kneading meat with meat to improve mastication and digestibility, and an antioxidant and nitrite- It is a very useful invention in the food processing industry because it has an excellent effect of providing a crispy sauce to be removed.
Claims (5)
Extracting persimmon, kiwi, pear, and liquor respectively; Lyophilizing each of the extracts obtained in the above step to form a powder; And mixing the powder obtained above in a weight ratio of 2: 3: 3: 2.
A granular powder prepared by the method of claim 2 and having antioxidant and nitrite removal activity.
A safflower sauce characterized by containing an acetic acid powder having antioxidative and nitrite-removing activity as defined in claim 3 as an active ingredient.
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