KR101459530B1 - Methods of treating poxviral infections - Google Patents

Abstract

The present invention provides a method of treating a disease or condition caused or associated with a virus belonging to the genus Poxvirus using an imino sugar (e. G. A DNJ derivative).

Description

METHODS OF TREATING POXVIRAL INFECTIONS < RTI ID = 0.0 >

Related application

The present application is a continuation-in-part of U.S. Pat. 61 / 272,252, which is incorporated herein by reference in its entirety.

Field

The present invention relates to the use of imino sugars for the treatment and / or prevention of viral infections with imino sugars and imino sugars, in particular viral infections caused by or associated with viruses belonging to the genus Poxvirus.

의 화합물One embodiment comprises administering to a subject in need of treatment or prevention of a disease or condition caused by or associated with a virus belonging to the poxvirus an effective amount of a compound of formula

Gt;

(here,

이고;R is selected from a substituted or unsubstituted alkyl group, a substituted or unsubstituted cycloalkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted oxaalkyl group; Or R is

ego;

R ₁ is a substituted or unsubstituted alkyl group;

X _1-5 is independently selected from H, NO ₂ , N ₃ or NH ₂ ;

Y is absent or a substituted or unsubstituted C ₁ -alkyl group other than carbonyl;

Z is selected from a bond or NH; With the proviso that when Z is a bond, Y is absent and when Z is NH, Y is a substituted or unsubstituted C ₁ -alkyl group other than carbonyl;

W _1-4 is hydrogen, a substituted or unsubstituted alkyl group, a substituted or unsubstituted haloalkyl group, a substituted or unsubstituted alkanoyl group, a substituted or unsubstituted aroyl group, or a substituted or unsubstituted haloalkane Selected independently from the furnace machine)

Or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for treating or preventing said disease or condition.

의 화합물Another embodiment provides a method of treating a cell infected with a virus belonging to < RTI ID = 0.0 > poxvirus &

Gt;

(here,

이고;R is selected from a substituted or unsubstituted alkyl group, a substituted or unsubstituted cycloalkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted oxaalkyl group; Or R is

ego;

R ₁ is a substituted or unsubstituted alkyl group;

X _1-5 is independently selected from H, NO ₂ , N ₃ or NH ₂ ;

Y is absent or a substituted or unsubstituted C ₁ -alkyl group other than carbonyl;

Z is selected from a bond or NH; With the proviso that when Z is a bond, Y is absent and when Z is NH, Y is a substituted or unsubstituted C ₁ -alkyl group other than carbonyl;

W _1-4 is hydrogen, a substituted or unsubstituted alkyl group, a substituted or unsubstituted haloalkyl group, a substituted or unsubstituted alkanoyl group, a substituted or unsubstituted aroyl group, or a substituted or unsubstituted haloalkane Selected independently from the furnace machine)

Or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable salt thereof.

Figures 1A-E illustrate the following imino sugars: A) N-butyldeoxynojirimycin (NB-DNJ, UV-1); B) N-nonyl deoxynojirimycin (NN-DNJ, UV-2); C) N- (7-oxadecyl) deoxynojirimycin (N7-O-DNJ, UV-3); D) N- (9-methoxynonyl) deoxynojirimycin (UV-4); E) The chemical structure of N- (N- {4'-azido-2'-nitrophenyl} -6-aminohexyl) deoxynojirimycin (UV-5).
Figure 2 is a synthetic reaction scheme for NN-DNJ.
Figures 3A-D illustrate the synthesis of N7-O-DNJ. In particular, Figure 3A shows the sequence of reactions leading to N7-O-DNJ; Figure 3B illustrates the preparation of 6-propyloxy-1-hexanol; Figure 3C illustrates the preparation of 6-propyloxy-1-hexanol; Figure 3D illustrates the synthesis of N7-O-DNJ.
Figures 4A-C relate to the synthesis of N- (9-methoxynonyl) deoxynojirimycin. In particular, Figure 4A illustrates the preparation of 9-methoxy-1-nonanol; Figure 4B illustrates the preparation of 9-methoxy-1-nonanal; Figure 4C illustrates the synthesis of N- (9-methoxynonyl) deoxynojirimycin.
Figure 5 shows in vivo survival data for mice infected with cow's virus.
Figure 6 shows in vivo safety data for UV-4 and UV-5.

Related Application

The following patent documents, all of which are incorporated herein by reference, may be useful in understanding this disclosure.

1) US Patent No. 6,545,021;

2) US Patent No. 6,809,803;

3) US Patent No. 6,689,759;

4) US Patent No. 6,465,487;

5) US Patent No. 5,622,972;

6) U.S. Patent Application No. 12 / 656,992, filed February 22, 2010;

7) U.S. Patent Application No. 12 / 656,993, filed February 22, 2010;

8) U.S. Patent Application No. 12 / 813,882, filed June 11, 2010;

9) U.S. Provisional Application No. 61 / 282,507, filed February 22, 2010;

10) U.S. Provisional Application No. 61 / 272,252, filed September 4, 2009;

11) U.S. Provisional Application No. 61 / 272,253, filed September 4, 2009;

12) U.S. Provisional Application No. 61 / 272,254, filed September 4, 2009;

13) U.S. Provisional Patent Application No. 61 / 282,508, filed February 22, 2010;

14) U.S. Provisional Patent Application No. 61 / 353,935, filed on June 11, 2010.

Definition of Terms

Unless otherwise specified, the singular form "means one or more ".

As used herein, the term "viral infection" means that a virus penetrates into healthy cells and proliferates or replicates using cell propagation machinery and ultimately lyses cells, resulting in apoptosis, release of viral particles, and other Refers to a disease state that causes infection of the cell. Latent infection by certain viruses is also a possible consequence of viral infection.

As used herein, the term " treatment or prevention of a viral infection "refers to inhibiting the replication of a particular virus, inhibiting viral delivery, or preventing the virus from becoming lodged in the host, It means relieving or alleviating the symptoms. Treatment is considered therapeutic if there is a reduction in virus load, a decrease in mortality and / or morbidity.

IC50 or IC90 (inhibition concentration 50 or 90) is the concentration of the therapeutic agent, e.g., imino sugar, used to achieve a 50% or 90% reduction in viral infection, respectively.

Description

The present inventors have found that certain imino sugars, such as deoxynojirimycin derivatives, may be effective against viruses belonging to the genus Poxvirus.

In particular, such imino sugars may be useful in preventing or treating a disease or condition caused by or associated with a virus belonging to the poxvirus.

Poxviruses include Codopopsvirus subfamily and Entomopoxvirus subfamily. Codopoxvirus subfamilies belong to the genus Orthofox, Parafox; Abiro fox; Capri viruses; Repor pox virus genus; The genus Sui of the virus; Moruccus foxvirus genus and Yata fox genus. Entomopoxvirus subfamily includes entomopoxviruses A, B and C. Viruses in OrthoFox, Parafox, Yata Fox, and Molly Fox can infect humans.

The virus belonging to the Orthopox virus with the Fox virus, Orthopoxvirus, belongs to the genus Buffalo virus; Carmel < / RTI > Cow fox virus; Actromelia virus; Monkey < / RTI > Rabbit < / RTI > Raccoon fox virus; Sealpox virus; Skunk Fox virus; Tetra-pox virus; Elegans Shin-Kushi disease virus; Vaccinia virus; Variola virus; And the Volepox virus.

Diseases caused by or associated with orthopoxvirus include: buffalo fox; Carmel Fox; Cow fox; Mouse fox (caused by Actrophyla virus); Monkey Fox; Rabbit Fox (also known as Green Rabbit Syndrome); Raccoon fox; Silox fox; Skunk Fox; Tetrafox; Elegans Shin Kushi disease; Small Fox; And Bowl Fox.

Viruses belonging to paroxifyline with the poxviruses, namely parapoxviruses, include Orff virus, pseudokaufox and bovine stomatitis virus.

Diseases caused by or associated with parapoxvirus include Orff, pseudoxauex, and bovine stomatitis.

Viruses belonging to the genus Yata Fox with the Fox virus, i.e., the yata pox virus, include the tanapoxvirus and the yabamonkey tumor virus.

A contagious, continuous species virus is an example of a virus belonging to the genus Molus cv.

In many embodiments, the imino sugar can be an N-substituted deoxynojirimycin. In some embodiments, such N-substituted deoxynojirimycin may be a compound of the formula:

Wherein W _1-4 is hydrogen, a substituted or unsubstituted alkyl group, a substituted or unsubstituted haloalkyl group, a substituted or unsubstituted alkanoyl group, a substituted or unsubstituted aroyl group, Lt; RTI ID = 0.0 > haloalkanoyl < / RTI >

In some embodiments, R may be selected from a substituted or unsubstituted alkyl group, a substituted or unsubstituted cycloalkyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted oxaalkyl group.

In some embodiments, R can be a substituted or unsubstituted alkyl group comprising from 1 to 16 carbon atoms, from 4 to 12 carbon atoms or from 8 to 10 carbon atoms, and / or a substituted or unsubstituted oxalkyl group . The term "oxaalkyl" refers to alkyl derivatives which may contain from 1 to 5 or from 1 to 3 or from 1 to 2 oxygen atoms. The term "oxalkyl" includes hydroxy-terminal alkyl derivatives and methoxy-terminal alkyl derivatives.

In some embodiments, R is _{- (CH 2) 6 OCH 3} , - (CH 2) 6 OCH 2 CH 3, - (CH 2) 6 O (CH 2) 2 CH 3, - (CH 2) 6 O ( CH ₂ ) ₃ CH ₃ , - (CH ₂ ) ₂ O (CH ₂ ) ₅ CH ₃ , - (CH ₂ ) ₂ O (CH ₂ ) ₆ CH ₃ ; - (CH ₂ ) ₂ O (CH ₂ ) ₇ CH ₃ ; - (CH _{2) 9} -OH; - (CH ₂ ) ₉ OCH ₃ , but is not limited thereto.

In some embodiments, R can be a branched or unbranched, substituted or unsubstituted alkyl group. In certain embodiments, the alkyl group is a C6-C20 alkyl group; A C8-C16 alkyl group; Or a long chain alkyl group which may be a Cg-Cio alkyl group. In some embodiments, R may be a long chain alkyl group, which may contain from 1 to 5 or from 1 to 3 or from 1 to 2 oxygen atoms.

In some embodiments, R may have the formula:

In this formula,

R ₁ is a substituted or unsubstituted alkyl group;

X _1-5 is independently selected from H, NO ₂ , N ₃ or NH ₂ ;

Y is absent or a substituted or unsubstituted C ₁ -alkyl group other than carbonyl;

Z is selected from a bond or NH; Provided that when Z is a bond, Y is absent and when Z is NH, Y is a substituted or unsubstituted C ₁ -alkyl group other than carbonyl.

In some embodiments, Z is NH and R ₁ -Y is a substituted or unsubstituted alkyl group such as a C 2 -C 20 alkyl group or a C 4 -C 12 alkyl group or a C 4 -C 10 alkyl group.

In some embodiments, X ₁ is NO ₂ and X ₃ is N ₃ . In some embodiments, each of X ₂ , X _4, and X ₅ is hydrogen.

In some embodiments, the imino sugar is U.S. Or a DNJ derivative disclosed in Patent Application Publication No. 2007/0275998 (incorporated herein by reference).

In some embodiments, the imino sugar may be one of the compounds shown in Fig.

Methods for synthesizing deoxynojirimycin derivatives are described, for example, in U.S. Pat. Patent Nos. 5,622,972, 5,200,523, 5,043,273, 4,994,572, 4,246,345, 4,266,025, 4,405,714 and 4,806,650, and U.S. Pat. 2007/0275998 (all of which are incorporated herein by reference).

In some embodiments, the imino sugar may be in the form of a salt derived from an inorganic or organic acid. Pharmaceutically acceptable salts and methods for preparing salt forms are described, for example, in Berge et al. (J. Pharm. Sci. 66: 1-18, 1977). Examples of suitable salts include the following salts: acetates, adipates, alginates, citrates, aspartates, benzoates, benzenesulfonates, nisulfates, butyrates, camphorates, camphorsulfonates, digluconates, cyclopentanepropio Such as sodium carbonate, potassium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, sodium carbonate, But are not limited to, sulfonate, lactate, maleate, methanesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, palmoate, pectinate, persulfate, 3- phenylpropionate, But are not limited to, sodium, succinate, tartrate, thiocyanate, tosylate, mesylate and undecanoate, But is not limited thereto.

In some embodiments, imino sugars may also be used in the form of prodrugs. Prodrugs of DNJ derivatives, such as the 6-phosphorylated DNJ derivatives, No. 5,043,273 and 5,103,008.

In some embodiments, imino sugars can be used as part of a composition, which further comprises a pharmaceutically acceptable carrier and / or an ingredient useful for delivering the composition to an animal. Numerous pharmaceutically acceptable carriers useful for delivering the composition to humans, and other ingredients useful for delivering the composition to other animals, such as cattle, are well known in the art. It is within the ordinary skill in the art to add such carriers and components to the compositions of the present invention.

In some embodiments, the pharmaceutical composition may consist essentially of an N-substituted deoxynojirimycin, which may mean that the N-substituted deoxynojirimycin is the only active ingredient in the composition.

In another embodiment, the N-substituted deoxynojirimycin may be administered with one or more additional antiviral compounds.

In some embodiments, imino sugars can be used in liposome compositions such as those disclosed in US Publications Nos. 2008/0138351 and 2009/0252785, as well as US Application No. 12/732630 (filed March 26, 2010).

Imino sugars, such as DNJ derivatives, may be administered to cells or animals infected with the virus. The iminosugar can inhibit the morphogenesis of the virus or treat the individual. Treatment can reduce, attenuate, or reduce the viral infection in an animal.

Animals that can be infected with the virus include mammals such as bovine, such as buffalo, sheep, goats and cows; camel; Rodents such as mice, rodents and zebras; Rabbits, such as rabbits and horses; Raccoon; seal; skunk; Horses, such as horse; Primates, such as monkeys and humans.

The amount of imino sugar administered to the animal or animal cells by the method of the present invention may be an amount effective to inhibit morphogenesis in the cells of the poxvirus. As used herein, the term "inhibition" may refer to the detectable reduction and / or elimination of the biological activity present in the absence of imatinos. The term "effective amount" can refer to the amount of imino sugar needed to achieve the specified effect. The term "treatment ", as used herein, refers to reducing or alleviating a symptom in a subject, preventing worsening or progressive symptoms, inhibiting or eliminating a causative agent, or infection or disorder associated therewith in a non- Can be prevented.

Thus, for example, the treatment of a disease caused by or associated with a virus can include destruction of the infectious agent, inhibition or inhibition of its growth or maturation, and neutralization of its pathological effects. The amount of imino sugar that can be administered to a cell or animal is preferably an amount that does not induce any toxic effects beyond those associated with its administration.

The actual dosage level of the active ingredient of the pharmaceutical composition may be varied to administer the amount of active compound (s) effective to achieve the desired therapeutic response in a particular patient.

The selected dose level will depend on the activity of the iminosugar, the route of administration, the severity of the condition being treated, and the condition and previous history of the patient being treated. It is within the skill in the art, however, to start the dose of the compound (s) at a level lower than that required to achieve the desired therapeutic effect, and to gradually increase the dose until the desired effect is achieved. If desired, an effective daily dose may be divided into multiple doses for administration purposes, for example, two to four doses per day. It will be understood, however, that the specific dose level for any particular patient will vary depending upon various factors, including weight, overall health, diet, time and route of administration, and combination with other therapeutic agents, and the severity of the condition or disease being treated will be. In some embodiments, the adult human daily dose can range from about 1 microgram to about 1 gram, or from about 10 mg to 100 mg, per imino of imino, per 10 kilogram body weight. In some embodiments, the total daily dose is 0.1 mg / kg body weight to 100 mg / kg body weight, or 1 mg / kg body weight to 60 mg / kg body weight, or 2 mg / kg body weight to 50 mg / mg / kg body weight to 30 mg / kg body weight. The daily dose may be administered via one or more dosing events throughout the day. For example, in some embodiments, the daily dose may be divided over an administration event twice a day (BID), three times a day (TID), or four times a day (QID) . In certain embodiments, a single dose event dose ranging from 1 mg / kg body weight to 10 mg / kg body weight is administered to a human by BID or TID to provide a dose of 2 mg / kg body weight to 20 mg / kg body weight, or 3 mg / kg body weight to 30 mg / kg body weight for a total of 1 day. Of course, the amount of imino sugar that must be administered to a cell or animal can vary depending on a number of factors well understood by those skilled in the art, such as the molecular weight of imino sugars and the route of administration.

Pharmaceutical compositions useful in the methods of the present invention may be administered systemically, such as oral solid preparations, ophthalmic preparations, suppositories, aerosols, topical agents or other similar agents. For example, it may be in the form of a powder, a tablet, a capsule, a lozenge, a gel, a solution, a suspension, a syrup, and the like. In addition to imino sugars, the pharmaceutical compositions may contain pharmaceutically acceptable carriers and other ingredients known to facilitate and enhance drug administration. Other possible agents such as nanoparticles, liposomes, re-sutured red blood cells, and immunology based systems may also be used to administer imino sugars. Such pharmaceutical compositions can be administered by a number of routes. The term "parenteral " as used herein includes, but is not limited to, subcutaneous, intravenous, intraarterial, intrathecal, and injection and infusion techniques. By way of example, the pharmaceutical composition may be administered by oral, topical, parenteral, systemic or pulmonary routes.

These compositions may be administered in a single dose, or in multiple doses administered at different times. Since the inhibitory effect of the composition against the poxvirus can be sustained, the dosage regimen can be adjusted so that the host cell is minimally affected while the virus proliferation is delayed. By way of example, animals can be dosed once weekly with a dose of the composition of the invention, whereby host cell function is suppressed only once a week for a short period of time, while virus proliferation is delayed throughout the week.

The embodiments described herein are further exemplified by the working examples below, but are not so limited in any way.

Working Example

1. Synthesis of N-nonyl DNJ

Procedure: DNJ (500 mg), ethanol (100 mL), furnace (530 mg) and acetic acid (0.5 mL) were charged to a 50-mL one-necked round bottom flask equipped with magnetic stirrer at room temperature. The reaction mixture was heated to 40-45 < 0 > C and stirred under nitrogen for 30-40 min. The reaction mixture was cooled to ambient temperature and Pd / C was added. The reaction flask was evacuated and replaced with hydrogen gas in the balloon. This procedure was repeated three times. Finally, the reaction mixture was stirred overnight at ambient temperature. The progress of the reaction was monitored by TLC (Note 1). The reaction mixture was filtered through a pad of celite and washed with ethanol. The filtrate was concentrated in vacuo to give a crude product. The crude product was purified by column chromatography (230-400 mesh silica gel). The product was eluted from the column using a solvent gradient of methanol in dichloromethane (10- > 25%). All fractions containing the desired product were combined and concentrated in vacuo to give the pure product (420 mg). The completion of the reaction was monitored by thin layer chromatography (TLC) using a thin layer silica gel plate (eluent: methanol: dichloromethane = 1: 2).

2. Synthesis of N-7-oxadecyl DNJ

2a. Synthesis of 6-propyloxy-1-hexanol

Procedure: A 500-mL one-neck round bottom flask equipped with magnetic stirrer was charged with 1,6-hexanediol (6.00 g), potassium tert-butoxide (5.413 g) at room temperature. The reaction mixture was stirred for 1 hour and then 1-iodopropane (8.63 g) was added. The reaction mixture was heated to 70-80 < 0 > C and stirred overnight. The progress of the reaction was monitored by TLC (Note 1). After the reaction was complete, water was added to the reaction mixture and extracted with ethyl acetate (2 x 100 mL). The combined organic layers were concentrated in vacuo to give a crude product. The crude product was dissolved in dichloromethane, washed with water, then brine, and dried over sodium sulfate. The organic layer was concentrated in vacuo to give a crude product. The crude product was purified by column chromatography using 230-400 mesh silica gel. The product was eluted from the column using a solvent gradient (10-45%) of ethyl acetate in hexanes. All fractions containing the pure desired product were combined and concentrated in vacuo to give pure 6-propyloxy-1-hexanol (lot D-1029-048, 1.9 g, 25%). The completion of the reaction was monitored by thin layer chromatography (TLC) (eluent: 60% ethyl acetate in hexanes).

2b. Preparation of 6-propyloxy-1-hexanal

(1.0 g), PDC (4.7 g), dichloromethane (10 mL) and celite (1.0 g) were added to a 50-mL 1-necked round bottomed flask equipped with a magnetic stirrer at room temperature. , And sodium acetate (100 mg). The reaction mixture was stirred at room temperature under nitrogen for 5 minutes. PDC (4.70 g) was added to the reaction mixture and stirred overnight. The progress of the reaction was monitored by TLC (Note 1). After the reaction was complete, the reaction mixture was loaded directly onto a column (230-400 mesh silica gel). The product was eluted from the column using a solvent gradient of dichloromethane in ethyl acetate (10- > 20%). All fractions containing the pure desired product were combined and concentrated in vacuo to give pure 6-propyloxy-1-hexane (Lot D-1029-050, 710 mg, 71%). The completion of the reaction was monitored by thin layer chromatography (TLC) (eluent: 60% ethyl acetate in hexanes).

2c. Synthesis of N-7-oxadecyl-DNJ

Procedure: A 50-mL 1-neck round bottom flask equipped with magnetic stirrer was charged with DNJ (500 mg), ethanol (15 mL), 6-propyloxy-1-hexane (585 mg) and acetic acid (0.1 mL) . The reaction mixture was heated to 40-45 < 0 > C and stirred under nitrogen for 30-40 min. The reaction mixture was cooled to ambient temperature and Pd / C was added. The reaction flask was evacuated and replaced with hydrogen gas in the balloon. This procedure was repeated three times. Finally, the reaction mixture was stirred overnight at ambient temperature. The progress of the reaction was monitored by TLC (Note 1). The reaction mixture was filtered through a pad of celite and washed with ethanol. The filtrate was concentrated in vacuo to give a crude product. The crude product was purified by column chromatography (230-400 mesh silica gel). The product was eluted from the column using a solvent gradient of methanol in dichloromethane (10-40%). All fractions containing the desired product were combined and concentrated in vacuo to give the pure product (lot: D-1029-052 (840 mg)). The completion of the reaction was monitored by thin layer chromatography (TLC) (eluent: 50% methanol in dichloromethane).

3. Synthesis of N- (9-methoxy) -nonyl DNJ

3a. Preparation of 9-methoxy-1-nonanol

Procedure: To a 500-mL 1-neck round bottom flask equipped with magnetic stirrer and stir bar was added 1,9-nonanediol (10.00 g, 62.3 mmol) in dimethyl sulfoxide (100 mL) and H ₂ O (100 mL) I filled it. A solution of sodium hydroxide (5.0 g, 125.0 mmol) in H ₂ O (10 mL) was added slowly at room temperature. During the addition of sodium hydroxide, the reaction mixture generated heat and the temperature rose to about 40 ° C. The mixture was stirred for 1 hour and then dimethylsulfate (16.52 g, 131 mmol) was added in four portions while maintaining the temperature of the reaction mixture at about 40 < 0 > C. The reaction mixture was stirred overnight at room temperature. The progress of the reaction was monitored by TLC (Note 1). TLC monitoring showed the reaction to be 25% conversion. At this stage additional dimethylsulfate (24.78 g, 196.44 mmol) was added and the resulting mixture was stirred at room temperature for a further 24 hours. After the reaction was complete, sodium hydroxide (10% solution in water) was added to the reaction mixture to adjust the pH of the solution to 11-13. The mixture was stirred at room temperature for 2 hours and extracted with dichloromethane (3 x 100 mL). The combined organic layers were washed with H ₂ O (200 mL), brine (150 mL), dried over anhydrous sodium sulfate (20 g), filtered and concentrated in vacuo to give the crude product (14 g). The crude product was purified by column chromatography using 250-400 mesh silica gel. The product was eluted from the column using a solvent gradient of ethyl acetate in hexane (10-50%). All fractions containing the pure desired product were combined and concentrated in vacuo to give pure 9-methoxy-1-nonanol (Lot D-1027-155, 2.38 g, 21.9%). The completion of the reaction was monitored by thin layer chromatography (TLC) using a thin layer silica gel plate (eluent: 60% ethyl acetate in hexanes).

3b. Preparation of 9-methoxy-1-nonane

Procedure: 9-Methoxy-nonanol (1.0 g, 5.9 mmol), dichloromethane (10 mL), molecular sieves (1.0 g, 3A) were added to a 50-mL 1-neck round bottom flask equipped with magnetic stirrer and stir bar at room temperature. , And sodium acetate (0.1 g). The reaction mixture was stirred at room temperature under nitrogen for 5 minutes. The reaction mixture was charged with pyridinium dichromate (4.7 g, 12.5 mmol) and stirred overnight. The progress of the reaction was monitored by TLC (Note 1). After the reaction was completed, the reaction mixture was filtered through a silica gel layer (about 15 g). The filtrate was evaporated in vacuo to give the crude compound. Which was purified by column chromatography using a silica gel column (250-400 mesh, 40 g). The product was eluted from the column using a solvent gradient of ethyl acetate in hexane (10-50%). All fractions containing the pure desired product were combined and concentrated in vacuo to yield 9-methoxy-nonane (lot: D-1027-156, 553 mg, 54.4%). The completion of the reaction was monitored by thin layer chromatography (TLC) using a thin layer silica gel plate (eluent: 60% ethyl acetate in hexanes).

3c. Synthesis of N- (9-methoxy) -nonyl DNJ

Procedure: DNJ (300 mg, 1.84 mmol), ethanol (20 mL), 9-methoxy-1-nonanal (476 mg, 2.76 mmol) was added to a 50-mL 2-necked round bottom flask equipped with magnetic stirrer and stir bar at room temperature. ). The reaction mixture was stirred under nitrogen for 5-10 minutes and Pd / C was added at room temperature. The reaction mixture was evacuated and replaced with hydrogen gas in the balloon. This process was repeated three times, and then the reaction mixture was stirred under atmospheric hydrogen at room temperature. The progress of the reaction was monitored by TLC (Note 1). The reaction mixture was filtered through a celite layer and washed with ethanol (20 mL). The filtrate was concentrated in vacuo to give a crude product. The crude product was purified by column chromatography using 250-400 mesh silica gel (20 g). The product was eluted from the column using a solvent gradient of methanol in ethyl acetate (5 - > 25%). All fractions containing the pure desired product were combined and concentrated in vacuo to give an off-white solid. The solid was triturated in ethyl acetate (20 mL), filtered and dried under high vacuum to give a white solid (Lot: D-1027-158 (165.3 mg, 28.1%)). The completion of the reaction was monitored by thin layer chromatography (TLC) using a thin layer silica gel plate (eluent: 50% methanol in dichloromethane).

4. Effect of Iminosugar on Vaccinia virus

NJ-DNJ (UV-1), NN-DNJ (UV-2), N7-O-DNJ (UV- To provide data on the inhibition of infectivity of Vaccinia virus.

<Table 7>

step. Compound screening for inhibition of infectious virus production was performed on UV compounds at concentrations ranging from 4 [mu] M to 250 [mu] M. Orthopoxvirus vaccinia NYCBOH virus strains were evaluated for viral suppression. BSC-40 cells (Burkitt Monkey kidney epithelial cell line) were obtained from the American Type Culture Collection (ATCC, Manassas, Va.). Cells were cultured in a 24-well flat bottom plate in a cell culture incubated in a 5% CO ₂ incubator at 37 ° C, with 5% fetal bovine serum, 2 mM L-glutamine, 100 U / ml penicillin, 100 [mu] g / ml streptomycin (MEM, Gibco). Cells were pretreated with compounds of final concentration 0.5% DMSO for 1 hour and then virus inoculum in EMEM containing 5% FBS was added. Three days later, the supernatant containing the virus was collected and subjected to a 10-fold dilution of the virus-containing supernatant in the viral plaque assay. For the activity, a 12-well plate containing BSC-40 cells at an 80% complete culture rate in growth medium was used. Virus supernatants were diluted from ^10-3 to ^10-8 , added to the cells, and incubated at 37 [deg.] C for 1 hour (with shaking every 5-10 minutes). Aspirating the virus infection medium and, 2X MEM (5% fetal calf serum final concentration) in a 1: 1 pre-mixed in - heating a 2% low melting point and replaced with an agarose 1 mL, in 37 ℃, 5% CO _{2 2} After incubation for one day, plaques were visualized by neutral red staining.

Example 5

The study assessed the efficacy of the imino sugar compound, UV-4, in the survival of mice vaccinated with cow Fox Brighton. This compound was tested in both in vitro (CC50: 125 to> 2,000 uM) and in vivo (both weight loss or no adverse effects observed in multiple mouse studies) and was shown to possess low toxicity. In this study, the compound was administered as a free drug dissolved in water. The UV-4 compounds were administered via the oral route (twice daily, intra-stomally via the oral gavage-IG) for a total of 10 days after the start of compound administration. The study animals were infected with cow Fox Brighton 1 hour prior to the first UV-4 administration (intranasal into LD90 (1.00e6 pfu / mouse) of about 1).

Way:

Infection: Female BALB / C mice aged 4 to 6 weeks were anesthetized with isofluorene and inoculated with 100 uL of cow Fox Brighton at a concentration of 1xLD90 (where did this virus strain come from, is this officially available?).

Administration: Twice a day, mice (n = 10) were orally administered the compound diluent (prepared in H ₂ O), 100 μl, orally. Treatment was continued for 10 days.

result:

Figure 5 shows survival data for mice infected with a 1xLD90 dose of cow Fox Brighton followed by water (control) or UV-4 (treated) three times a day for 10 days. Table 8 shows the percent survival of mice in the groups indicated in the left column a) water treated control group and b) UV-4 treated group. Each control and treatment group included 10 mice.

Kaplan-Meier analysis of control and UV-4 treated groups. A log-rank (Mantel Cox) analysis showing the p-values between the arms. A p value of <0.05 indicates significance. The mouse P-value for UV-4 0.2 mg was 0.046.

Example 6

Immediate safety study

Methods and Discussion: BALB / c and C57 / Bl / 6 mice were treated with an oral suspension of UV-1, UV-4, UV-5 [2 times a day for 7 days, 100 μl per mouse for 7 days 100 and 10 mg / kg (2 and 0.2 mg / mouse, respectively) at 8 hour intervals], weight loss and overall health. Seven days after treatment, the mice showed no significant signal of weight loss as compared to the "vehicle only" control. The results of these experiments are shown in Fig.

When BALB / c mice were treated with the highest concentration of UV-5, they did not show signs of weight loss, but showed diarrhea, red urine, and harsh looks. C57 / Bl / 6 mice exhibited the same symptoms except for the matted appearance. These symptoms were promptly discontinued at the time of treatment and BALB / c mice in these groups were very healthy until day 11 (4 days after compound treatment).

Conclusion: These compounds have been shown to be relatively non-toxic in the mouse model and compounds at these concentrations were considered safe.

* * *

While the foregoing is directed to certain preferred embodiments, it will be understood that the invention is not limited thereby. Those skilled in the art will appreciate that various modifications may be made to the disclosed embodiments and that such modifications are intended to be included within the scope of the present invention.

All publications, patent applications, and patents, all of which are incorporated herein by reference, are incorporated herein by reference in their entirety.

Claims (19)

A pharmaceutical composition for treating or preventing a disease or condition caused by a virus belonging to the genus Poxvirus in a subject, comprising a compound of the formula: or a pharmaceutically acceptable salt thereof.
&Lt;

(here,
R is C ₁ -C ₁₆ alkyl group or a C ₁ -C ₁₆ oxa selected from an alkyl group or; Or R is

ego;
R ₁ -Y is a C ₂ -C ₂₀ alkyl group;
X _1-5 is independently selected from H, NO ₂ , N ₃ or NH ₂ ;
Z is NH;
Each of W ₁ , W ₂ , W ₃ and W ₄ is hydrogen) The pharmaceutical composition of claim 1, wherein R is a C ₁ -C ₁₆ alkyl group or a C ₁ -C ₁₆ oxaalkyl group. _{7. The} pharmaceutical composition of claim 1, wherein R is a C6- _C12 alkyl or an oxalkyl group. The method of claim 1, wherein, R is C ₈ -C ₁₀ alkyl or oxa-alkyl due to the pharmaceutical composition. The method of claim 1, wherein the compound is selected from the group consisting of a) N-butyldeoxynojirimycin or a pharmaceutically acceptable salt thereof; b) N-nonyl deoxynojirimycin or a pharmaceutically acceptable salt thereof; c) N- (7-oxadecyl) deoxynojirimycin or a pharmaceutically acceptable salt thereof; or d) N- (9-methoxynonyl) deoxynojirimycin or a pharmaceutically acceptable salt thereof. 2. The compound of claim 1, wherein R is

&Lt; / RTI &gt; The method of claim 6, wherein X ₁ is NO _2, the pharmaceutical composition X ₃ is N _3. The method of claim 6, wherein each of X _2, X ₄ and X ₅ are hydrogen in the pharmaceutical composition. The pharmaceutical composition of claim 1, wherein said compound is N- (N- {4'-azido-2'-nitrophenyl} -6-aminohexyl) deoxynojirimycin or a pharmaceutically acceptable salt thereof. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the subject is a mammal. 11. The pharmaceutical composition according to claim 10, wherein the subject is a human. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the virus belongs to Orthopoxvirus. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the virus is vaccinia virus. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the virus is cow virus. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the virus is Variola virus. 10. The pharmaceutical composition according to any one of claims 1 to 9, wherein the disease or condition is small Fox. delete delete delete

Images