KR101437814B1 - Food Composition Comprising Fucoxanthin Derived from Microalgae and The Preparation Method thereof - Google Patents

Abstract

The present invention provides a food composition for improving liver functions and healing a hangover, and to a method for producing the same, wherein the food composition comprises: a fucoxanthin separated and refined from Phaeodactylum tricornutum or Isochrysis aff. galbana which is microalgae; and at least one extract of a wild flower selected from Justicia brandegeeana flowers, Cymbidium goeringii, Potentilla discolor Bunge, or Lychnis cognata Maxim.

Description

TECHNICAL FIELD The present invention relates to a food composition containing fucoxanthin derived from microalgae and a method for preparing the same.

More particularly, the present invention relates to a food composition containing microalgae-derived fucosanthin and a method for producing the same, and more particularly, to a method for producing microcurrent-derived fucoxanthin, which is capable of minimizing the loss of fucosanthin during purification from microalgae, To a food composition having a liver function and a hangover effect by being added as an additive to a composition, and a process for producing the same.

Recently, research has been reported that the ingredients contained in seaweeds are beneficial to human health. Particularly, the fuco xanthine component is a specific carotenoid found abundantly in brown algae, and although it has been reported to have some anti-obesity effect, no effect related to elimination of liver function or hangover has been reported.

It is known that fucosanthin can be obtained by extracting from a brown algae with acetone extraction method or hexane extraction method, and separating and purifying it. However, when fucosanthin is purified by this method, safety is low and the yield of fucozanthin is greatly decreased. But it is also pointed out that the supply is not smooth enough to be extended to an industrial scale such as the above.

[Prior Art Document Information]
1. Korean Patent Publication No. 10-526434 (Oct. 28, 2005)
2. Korean Patent Publication No. 10-828069 (Apr. 30, 2008)

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a fucoxanthin of high purity and high purity by minimizing the loss of fucosanthin during purification from microalgae, Function and a hangover effect, and a method for producing the same.

Another object of the present invention is to provide a food composition comprising four wildflower extracts which are selected by confirming the industrial availability of domestic wildflower and which are determined to improve the liver function and the hangover resolution effect of fucoxanthin And a method for producing the same.

The technical problem of the present invention as described above is achieved by the following means.

(1) fucoxanthin isolated from Phaeodactylum tricornutum or Isochrysis aff. Galbana in a microalgae and at least one selected from shrubs, A food composition for the improvement of liver function and a food for hangover which comprises one kind of extract.

(2) The method according to (1)

The above-mentioned shrimp flower, Lycoris spp., Cotton floricultur and Rhododendron flower extract are prepared by mixing L. fermentum and L. casei in a weight ratio of lyophilized powder to freeze-dried powder of shrimp, Wherein the extract is an ethanol extract of a fermentation product obtained by adding 0.1 to 1.0% by weight of a 6: 4 to 4: 6 mixed strain at 30 to 33 占 폚.

(3) a step of pulverizing Phaeodactylum tricornutum or Isochrysis aff. Galbana powder into fine algae by a ball mill, followed by ultrasonic extraction;

Adding a mixed solvent of petroleum ether, isopropyl alcohol and cyclohexane to the ultrasonic extract, filtering off the chlorophyll and obtaining the remaining solids;

Adding 70 to 75% ethanol to the solid to dissolve it, and then adsorbing and concentrating fucosanthin using an adsorption resin;

A step of eluting fucosanthin by adding 95 to 100% ethanol to the adsorbent resin adsorbed on the fucoxanthin;

Adding water at 60 to 70 캜 to the fucosanthin eluate, allowing the mixture to instantly cool to 30 to 40 캜, and allowing the mixture to stand for 2 to 3 hours to obtain fucosanthin crystals; And

Adding 0.1 to 10% by weight of the obtained fucoxanthin and 0.1 to 1.0% by weight of an extract of at least one selected from the group consisting of shrimp, mackerel, sunflower, and copepods to the food composition; A method of preparing a food composition useful for human consumption.

As described above, according to the present invention, it is possible to provide a food composition for improving liver function and eliminating hangover by adding fucosanthin at a high concentration and high purity by minimizing loss of fucosanthin in the process of refining from microalgae, .

1 is a process flow diagram of a food composition according to the present invention.

In order to achieve the above object, the food composition according to the present invention is characterized in that fucosanthin isolated from Phaeodactylum tricornutum (PT) or Isochrysis aff. Galbana (IG) As an active ingredient for improving and hangovers.

First, in order to use fucosanthin on an industrial scale, a refining technique for supplying a large amount of food additives should be preceded.

For this purpose, in order to enhance the extraction efficiency of fucosanthin from Phaeodactylum tricornutum or Isochrysis aff. Galbana to microalgae, the microcirculation is first pulverized with a ball mill, and then subjected to ultrasonic extraction . The fine algae are pulverized with a ball mill, and the ultrasonic wave extraction is preferably performed using an ultrasonic generator at 80 to 90 kHz for 10 to 20 minutes.

The ultrasound extract obtained as described above contains a large amount of impurities such as chlorophyll in addition to fucosanthin and it is necessary to remove these impurities from the ultrasound extract to remove the impurities.

In the present invention, a mixed solvent of petroleum ether, isopropyl alcohol and cyclohexane is used as an organic solvent for removing impurities including chlorophyll. Preferably, the petroleum ether, isopropyl alcohol and cyclohexane may be mixed in a weight ratio of 6: 2: 2 to 8: 1: 1. The mixed solvent is added to the ultrasonic extract, and impurities such as chlorophyll are filtered off by using a filter or the like to obtain a solid containing the remaining fucoxanthin.

The solid substance contains a large amount of fucoxanthine. In order to dissolve and extract fucoxanthin contained in the solid substance, ethanol is used in the present invention. At this time, 70 to 75% ethanol is used for the ethanol, and extraction efficiency can be improved by using an organic solvent in which 1: 1 by weight of butanol is mixed.

In order to obtain fucosanthin at a high concentration from the ethanol extract in which fucosanthin is dissolved as described above, an organic adsorption resin is used in the present invention. The organic adsorbent resin is preferably styrene divinylbenzene synthetic resin adsorbent such as Diaion HP20, HP21, SepharBiz SP70, SP700, SP825, SP850, SP207, Amberlite XAD2, XAD4, XAD16HP or methacrylic synthetic adsorbent resin Rhodiaion HP1MG, HP2MG and the like, Amberlite XAD7HP and the like can be used.

70-75% ethanol used for the extraction of fucosanthin from solids greatly improves the adsorption efficiency of fucoxanthin contained in the ethanol extract to the above organic adsorbent resin. Therefore, when the organic adsorbent resin is added to the ethanol extract, only fucosanthin can be adsorbed and separated at a high concentration.

After separating the organic adsorption resin adsorbed by fucoxanthin, 95 to 100% ethanol, preferably 100% ethanol, is added to elute fucosanthin adsorbed on the resin. The eluted product of fucosanthin thus obtained contains a large amount of fucosanthin at a high concentration.

In the present invention, a crystallization process is performed in order to purify fucosantine of high purity on a high concentration fucosanthin-containing eluate obtained through a two-step process of adsorption and elution with ethanol.

To this end, in the present invention, highly purified fucosanthin can be crystallized by adding water to the fucosanthin eluate at 60 to 70 ° C, allowing the solution to cool at 30 to 40 ° C for 2 to 3 hours.

Through the process described above, it is possible to supply fucosanthin to be used in various food compositions with an industrial scale at a high concentration and high purity of fuco xanthine which can be produced in a short time by a simple and inexpensive method in a large amount.

The high purity fucoxanthin obtained through the above process is added to the food composition in an amount of 0.1 to 20% by weight.

In a preferred embodiment of the present invention, a food composition for improving liver function and hangover resolution together with fucosanthin derived from microalgae is added to the food composition of the present invention, except for many wildflowers known to have liver function improvement or hangover resolution effect, And extracts of at least one species selected from the group consisting of shrimp grass flowers, lobsters, cotton wool and flower buds selected through experiments in search of species.

The wild-type extract according to the present invention includes a hot-water extract, an ethanol extract, or an extract using a mixture of water and ethanol, or a supercritical extract.

In the case of the hot-water extract, it is preferable to add 1 to 10 times of water and extract at 1 to 2 atm and 80 to 120 캜 for 1 to 12 hours. The ethanol extract may be an extraction solvent composed of 50 to 100% ethanol, and the supercritical extract may be performed under the pressure of 250 to 350 bar using ethanol as an extraction solvent.

The above wildflower can be safely used as a natural plant material in the present invention, and is excellent in liver function improvement and hangover resolution effect.

Preferably, the wild-type extract is a mixture of Lactobacillus perfringens L. fermentum and B. longum at a weight ratio of 4: 6 to 6: 4, To 0.1 to 1.0% by weight of fermented lactic acid bacteria at 30 to 33 ° C for 1 to 7 days, which is extracted with ethanol.

The wildflower extract is preferably added in an amount of 0.1 to 10% by weight based on the total weight of the food composition, and the effect of improving the liver function and hangover is further enhanced within the above range.

Examples of the food composition of the present invention include various foods, beverages, gums, tea, vitamin complexes, and health supplement foods.

The food composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and heavy stabilizers (cheese, chocolate etc.), pectic acid and its salts, , Organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the food compositions of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 30 parts by weight per 100 parts by weight of the food composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these embodiments are for the purpose of understanding the present invention and should not be construed as limiting the scope of the present invention.

EXAMPLES Purification of fucoxanthin

1 kg of the dried product of Phaeodactylum tricornutum or Isochrysis aff. Galbana was pulverized with a ball mill for 10 minutes in the microalgae and ultrasonicated for 10 minutes at 90 kHz using an ultrasonic generator . To remove the chlorophyll, a mixed solvent of 8: 1: 1 by weight of petroleum ether, isopropyl alcohol and cyclohexane was added to the ultrasonic extract obtained through the above process, and the mixture was filtered using a filter. 75% ethanol was added to the solid to dissolve sufficiently to obtain 0.5 kg of the extract. The ethanol extract was mixed with Amberlite XAD2 to adsorb and concentrate fucosanthin.

Through the above process, the adsorbent resin adsorbed on fucoxanthin was treated with 100% ethanol to elute fucosanthin adsorbed on the resin. Next, in order to increase the purity of fucosanthin, 70 ° C water was added to the fucosanthin eluate, and the solution was left to cool at 40 ° C for 2 hours to obtain fucosanthin crystals.

HPLC analysis was performed for the analysis of fucosanthin, using an Agilent 1200 HPLC apparatus for HPLC analysis and a methanol / water double solvent system for the solvent system. The column used was YMC carotenoid (250x4.5 mm, 3 μm) and the solvent gradient program was to raise methanol from 90% to 100% for 20 minutes and hold for 5 minutes. Detection of fucosanthin was carried out at 450 nm.

According to the above experimental results, the purity of fucoxanthin obtained by this Example was 99.2% for PT and 99.1% for IG.

[Example 1] Purification

The following ingredients were combined and tableted according to the conventional preparation method of tablets to prepare tablets.

ingredient content Fukosanthin (purity 99.8%) derived from microalgae PT 100 mg Hot water extract of shrimp flower 50 mg Hot spring water extract 50 mg Hot water extract of cotton floret 50 mg Hot spring water extract 50 mg Lactose 100 mg Starch 100 mg Magnesium stearate Suitable amount

[Example 2]

The following ingredients were blended and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

ingredient content Fucosantine from microalgae IG (purity 99.8%) 100 mg Hot water extract of shrimp flower 50 mg Hot spring water extract 50 mg Hot water extract of cotton floret 50 mg Hot spring water extract 50 mg Lactose 100 mg Starch 100 mg Talc 5 mg Magnesium stearate Suitable amount

[Example 3]

The following ingredients were mixed, filled into a container and sterilized to prepare a beverage.

ingredient content Fukosanthin (purity 99.8%) derived from microalgae PT 20g Hot water extract of shrimp flower 10g Hot spring water extract 10g Hot water extract of cotton floret 10g Hot spring water extract 10g Sugar 20g Isomer 20g Apple 2g Purified water Total volume of drink 1ℓ
control

[Example 4]

Four wildflower extracts were prepared by adding 1.0 wt% of mixed lactic acid bacteria (L. fermentum and L. casei in a weight ratio of 7: 3 by weight) to the lyophilized powder, The capsule was prepared in the same manner as in Example 2, except that the ethanol extract of the fermented product obtained by fermentation was used.

[Experimental Example 1] Test for improving liver function

In order to measure the detoxification degree of each preparation prepared according to Examples 1 to 4, the activity of GST, which is one of important liver detoxification mechanisms, was measured. Each of the samples prepared according to the examples was added to the prepared reaction reagent, followed by reaction at 37 ° C for 5 minutes. Then, 1-chloro-2,4-dinitrobenzene was added as a substrate and reacted at 37 ° C for 2 minutes. After the reaction, 20% TCA was added to terminate the reaction and centrifugation. The supernatant was taken and the absorbance at 340 nm was measured. The activity and the activity of GST were calculated as follows, and the results are shown in Table 1 below. In Comparative Example 1, the wild-type extract was not added in Example 2.

Total activity = (A340 / 9.6) x dilution times 占 (3 ml / 0.1) 占 crude extract (ml)

Inactive (Units / ml protein) = total active / total protein

Activity ratio (%) = inactivated _{experimental group} / inactive _{control group} 100

Liver function improvement (% / mg / ml) division Liver function improvement effect Example 1 322 Example 2 358 Example 3 354 Example 4 365 Comparative Example 1 153

As a result of the above experiment, it can be confirmed that the addition of the wild flower extract has a greater effect of improving the liver function as compared with the addition of fucoxanthin alone.

[Experimental Example 2] Hangover resolution test

As a result of measurement of alcohol decomposition effect according to a known method for each of the samples of Examples 1 to 4, the results as shown in Table 5 were obtained, and all the samples showed excellent alcohol decomposition effects. In Comparative Example 2, the wild-type extract was not added in Example 2.

Alcohol degradation effect (% / mg / ml) division Alcohololytic activity Example 1 65 Example 2 72 Example 3 69 Example 4 76 Comparative Example 2 43

As a result of the above experiment, it can be confirmed that the addition of the wild flower extract has a greater effect of decomposing alcohol than the addition of fucoxanthin alone.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (3)

Fucoxanthin isolated from Phaeodactylum tricornutum or Isochrysis aff. Galbana in the microalgae, and wildflower extract as a shrimp flower extract, Chochuwha extract, Cotton wilt extract and Rhizome flower extract A food composition for improving liver function and for preventing hangovers. The method according to claim 1,
The above-mentioned shrimp flower, Lycoris spp., Cotton floricultur and Rhododendron flower extract are prepared by mixing L. fermentum and L. casei in a weight ratio of lyophilized powder to freeze-dried powder of shrimp, Wherein the extract is an ethanol extract of a fermentation product obtained by adding 0.1 to 1.0% by weight of a 6: 4 to 4: 6 mixed strain at 30 to 33 占 폚. Pulverizing Phaeodactylum tricornutum or Isochrysis aff. Galbana powder into fine algae by a ball mill, and ultrasonically extracting the powder;
Adding a mixed solvent of petroleum ether, isopropyl alcohol and cyclohexane to the ultrasonic extract, filtering off the chlorophyll and obtaining the remaining solids;
Adding 70 to 75% ethanol to the solid to dissolve it, and then adsorbing and concentrating fucosanthin using an adsorption resin;
A step of eluting fucosanthin by adding 95 to 100% ethanol to the adsorbent resin adsorbed on the fucoxanthin;
Adding water at 60 to 70 캜 to the fucosanthin eluate, allowing the mixture to instantly cool to 30 to 40 캜, and allowing the mixture to stand for 2 to 3 hours to obtain fucosanthin crystals; And
0.1 to 1.0% by weight of the obtained fucoxanthin, 0.1 to 1.0% by weight of the shrimp flower extract, 0.1 to 1.0% by weight of the ginseng extract, 0.1 to 1.0% ≪ / RTI > wherein the method comprises the steps of:

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