KR101405053B1 - Apparatus and method for detecting target material using quantum dot - Google Patents

Apparatus and method for detecting target material using quantum dot Download PDF

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KR101405053B1
KR101405053B1 KR1020140034866A KR20140034866A KR101405053B1 KR 101405053 B1 KR101405053 B1 KR 101405053B1 KR 1020140034866 A KR1020140034866 A KR 1020140034866A KR 20140034866 A KR20140034866 A KR 20140034866A KR 101405053 B1 KR101405053 B1 KR 101405053B1
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quantum dots
solution layer
target material
tube
density solution
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변철우
문민오
김주현
장형구
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고센바이오비드 주식회사
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    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots

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Abstract

According to an embodiment of the present invention, as a device for analyzing a target material using quantum dots, provided is a device for analyzing a target material, which comprises a tube insertion hole in which a tube having a high-density solution layer formed at the lower part and a low-density solution layer formed at the upper part, where a target material, quantum dots and magnetic particles are mixed, is inserted; a magnetic force generating device which allows a complex of the target material, the quantum dots and the magnetic particles formed at the lower side of the tube insertion hole and combined in the low-density solution layer to move toward the lower part of the tube through the high-density solution layer; and a camera module responding to a specific wavelength to detect light emitted by the quantum dots of the complex in the high-density solution layer.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an apparatus and a method for detecting a target substance using quantum dots,

An embodiment of the present invention relates to an apparatus and a method for detecting a target material using quantum dots and, more particularly, to a method and apparatus for detecting a target material through light from a quantum dot forming a complex with a target material Apparatus and method.

Fluorescent materials are widely used for the detection of samples, study of materials, studies of physicochemical interactions, and biochemical studies of cells. On the other hand, a quantum dot (Quantum Dot), which is produced through a chemical synthesis process and occurs in a wavelength range where fluorescence is much stronger than a general fluorescent substance, has been developed.

The quantum dots are particles whose nano-sized II-IV semiconductor particles (CdSe, CdTe, CdS, etc.) form the core. The light from the quantum dots is the light generated by electrons falling from the conduction band to the valence band. Quantum dots show many properties different from general fluorescent dyes.

For example, quantum dots emit light of different wavelengths depending on the size of the particles, even if they consist of the same central material. The smaller the particle size, the shorter wavelength light is emitted. By adjusting the particle size using this property, the light of the desired wavelength can be emitted.

Due to the unique and useful optical properties of Qdots, fundamental studies of properties and interaction with other materials are actively being studied. So far, quantum dots have been developed largely as particles for application in biotech fields, but they are thought to be useful probes for researchers in materials engineering, chemistry, and chemical engineering. In particular, It is expected that various types of materials can be made.

Techniques for detecting various fungi, viruses, and proteins using light emitted from quantum dots have also been introduced in various forms.

Typically, there is "a method of detecting a target substance using selective aggregation of quantum dots ", which is disclosed in Korean Patent Application No. 2009-0043333.

This is a technique for detecting a target substance by utilizing the phenomenon that the quantum dots selectively flocculate on the target material. Specifically, when a target substance binds to a detection substance (i.e., a probe), electrostatic stabilization occurs and it can not bind to the quantum dots. When the target substance can not bind to the detection substance, It is a technique for detecting the presence or absence of a target substance by analyzing the selective aggregation of the quantum dots according to such properties.

However, it is necessary to directly detect the properties of different characteristics depending on the aggregation of the quantum dots. In other words, it is a technique that must follow the inconvenience of grasping the size change of the quantum dot due to cohesion and the change of the zeta potential.

Therefore, there is a need for a technique capable of detecting a target substance using a quantum dot in a more easy way.

SUMMARY OF THE INVENTION It is an object of the present invention to solve the above-described problems of the prior art.

In the present invention, a complex is formed by binding a quantum dot and magnetic particles to a target substance, and a complex attracted to a magnetic force is detected through quantum dot fluorescence, thereby enabling the target substance to be detected easily.

It is another object of the present invention to enable detection and identification of the presence of even a very small amount of target material and to omit sample preparation for target material detection.

According to an aspect of the present invention, there is provided an apparatus for analyzing a target material using quantum dots, the apparatus comprising: a high-density solution layer in a lower portion; a low-density solution in which a target material, a quantum dot, A tube insertion port into which the layered tube is inserted; A magnetic force generating device formed at a lower portion of the tube inserting port to cause a composite of the target substance, quantum dot, and magnetic particles bound in the low density solution layer to be led to the lower portion of the tube through the high density solution layer; And a camera module for detecting light emitted by quantum dots of the complex present in the dense solution layer in response to a specific wavelength.

Wherein the target material analyzing apparatus includes an information processing module for determining the presence or absence of the target material forming the complex together with the quantum dots according to the presence or amount of quantum dots determined based on the light detection result by the camera module .

The target material analyzing apparatus may further include a display unit for displaying information on the quantum dot or the target material determined by the information processing module.

The target material analyzing apparatus may further include a light irradiation unit for applying the specific wavelength to the tube.

The tube insertion port may be formed to have a narrow cross-sectional area as it approaches the magnetic force generating device.

According to another embodiment of the present invention, there is provided a method for analyzing a target material using a quantum dot, the method comprising: attaching a substance having an antibody or affinity to a specific target substance to a quantum dot and magnetic particles; Forming a high-density solution layer on the lower part of the tube, forming a low-density solution layer in which the quantum dots, the magnetic particles and the target material are mixed on the upper part, and forming a quantum dot having the antibody on the low- To form a complex with the target material; Applying a magnetic force to the lower portion of the tube so that the composite is led to the lower portion of the tube through the dense solution layer; And determining whether a quantum dot that emits light in response to the specific wavelength is present in the dense solution layer after applying a specific wavelength to the tube.

The attaching step may include attaching an antibody or affinity substance to the first target substance to a quantum dot having the first property and attaching a substance having an antibody or affinity to the second target substance to the quantum dot having the second property .

The target substance analyzing method may further include determining the presence or absence of the target substance based on the amount of quantum dots present in the high-density solution layer.

The target material analyzing method may further include, after the magnetic force application step, removing the low density solution layer.

The step of checking presence of the quantum dot may include the step of capturing light emitted from the quantum dot after applying the specific wavelength.

According to an embodiment of the present invention, a composite in which magnetic particles and quantum dots are combined with a target material is formed, and only a complex containing a target material is extracted using magnetic force. Then, the amount of the target material By judging, the presence and the amount of the target substance can be easily judged only by the properties of the light emitted from the quantum dots.

Further, according to the embodiment of the present invention, since the signal for detecting the target substance is amplified by the quantum dots and the concentration of the complex containing the target substance is naturally performed by the magnetic particles, even if a very small amount of the target substance is present Can be detected and identified.

Meanwhile, according to the present embodiment, the complex due to the specific reaction is naturally concentrated on the bottom of the tube by the magnetic force, without the process for eliminating the bond with the target material, that is, the specific binding excluding the specific binding, A sample preparation process for detection, and the like become unnecessary.

1 is a view illustrating a principle of analyzing a target material using quantum dots according to an embodiment of the present invention.
FIG. 2 is a view showing a configuration of a target material analyzing apparatus according to an embodiment of the present invention. FIG.
3 is a cross-sectional view showing a configuration of a target material analyzing apparatus according to an embodiment of the present invention.
4 is a flowchart illustrating a method of analyzing a target material using quantum dots according to an embodiment of the present invention.

The following detailed description of the invention refers to the accompanying drawings, which illustrate, by way of illustration, specific embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, certain features, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in connection with an embodiment. It is also to be understood that the position or arrangement of the individual components within each disclosed embodiment may be varied without departing from the spirit and scope of the invention. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is to be limited only by the appended claims, along with the full scope of equivalents to which such claims are entitled, if properly explained. In the drawings, like reference numerals refer to the same or similar functions throughout the several views.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can easily carry out the present invention.

1 is a view for explaining the principle of a method for analyzing a target material using quantum dots according to an embodiment of the present invention.

First, referring to FIG. 1A, a high-density solution layer 110 is formed on a tube 100, and a low-density solution layer 120 is formed on the high-density solution layer 110.

The term " high density " and " low density " refer to a degree of relative density. The high density solution layer 110 means a solution layer in which a solute having a relatively large molecular weight is dissolved compared to the low density solution layer 120, The solution layer 120 means a solution layer in which a solute having a relatively small molecular weight is dissolved as compared with the high density solution layer 110.

The high-density solution layer 110 and the low-density solution layer 120 can be naturally divided into the lower layer and the upper layer, respectively, according to the principle of the density gradient.

The high-density solution layer 110 may be formed of, for example, a solution of poly-ethylene glycol (PEG), a solution of sucrose, a solution of glycol, a solution of sodium iodide, a solution of cesium chloride, Percoll solution or the like. The high-density solution layer 110 functions as a buffer layer that becomes a movement path when the magnetic particles dispersed in the low-density solution layer 120 are attracted by the magnetic force applied to the lower portion of the tube 100, as described later.

The target substance (T), the magnetic particles (B), and the quantum dots (Q) to be detected are mixed in the low density solution layer (120). The target substance (T) may be any substance which is desired to be identified or diagnosed and examined. Fungi such as Salmonella typhimurium such as Salmonella typhi or Salmonella typhimurium, Escherichia coli, Vibrio cholera, Mycobacterium bovis, Shigella, Lactobacillus, Listeria monocytogenes and the like; Viruses such as influenza, HIV, HPV, and HCV; A substance comprising a specific protein; Nucleic acids such as DNA or RNA; Or other biopolymer material. The target material (T) can be replaced with all the biopolymer materials in addition to the examples enumerated above.

The target material (T) should be able to form a complex by linking with both the quantum dot (Q) and the magnetic particle (B). To this end, quantum dots (Q) and magnetic particles (B) should be attached to the target substance (T) with an antibody or affinity. A substance having an affinity may be, for example, a substance such as a probe of a complementary sequence to a target substance (T), wherein the probe of the complementary sequence may be a concept including both DNA or RNA. Attachment of a substance having an antibody or affinity to the quantum dot (Q) and the magnetic particle (B) can be performed using a covalent bond or a bond using an affinity.

For example, the magnetic particle (B) is conjugated with a target substance (T), that is, an antibody that binds to a specific antigen, and a quantum dot (Q) Can be attached. When the different types of antibodies are attached to the magnetic particles (B) and the quantum dots (Q), the magnetic particles (B) and the quantum dots (Q) both bind to the same target material (T) Since 'b' is a different antibody, the binding site for the target substance (T) is different. Therefore, in order to prevent the magnetic particles (B) and the quantum dots (Q) from competing with each other when binding to the target material (T), the kinds of antibodies attached to the magnetic particles (B) and the quantum dots .

Although only antibodies are described herein, different substances must be attached to the magnetic particles (B) and the quantum dots (Q) when attaching a substance having affinity to the target substance (T).

Since the target material T and the quantum dots Q and the target material T and the magnetic particles B must be combined in the low-density aqueous solution 120, the low-density aqueous solution 120 is mixed with the environment , Environment with an appropriate pH). ≪ / RTI > For example, the low-density aqueous solution 120 may be formed of a phosphate buffered saline, a Tris buffer, or the like.

On the other hand, the quantum dots Q and the magnetic particles B can be combined with the target material T to form a complex, but the quantum dots Q and the magnetic particles B can not bind to each other. That is, the quantum dot Q and the magnetic particles B form a complex only when the target substance T is present.

According to one embodiment, the magnetic particles (B) are selected from the group consisting of Mg, Mn, Al, Ba, Cu, Ni, Co, Fe, Cr, Zn, Nb, Mo, Pd, Ag, Cd, W, And may comprise at least one component. In one example, the magnetic particles (B), magnetite (Fe 3 O 4), hematite (α-Fe 2 O 3) , MAG H. boehmite (γ-Fe 2 O 3) , iron sesquioxide (β-Fe 2 O 3, Fe 2 O 3 ), iron oxide (FeO), ferrite (a form in which one Fe atom in Fe 3 O 4 is changed to another magnetic atom, for example, CoFe 2 O 4 , MnFe 2 O 4, etc.) Or one or more of them. The synthesis of the magnetic particles (B) can be carried out using one or more of the known methods.

Referring to FIG. 1 (b), binding between the target material T and the quantum dot Q in the low-density solution layer 120 and binding between the target material T and the magnetic particles B are induced. The quantum dots Q and the magnetic particles B are bonded to the surfaces of the quantum dots Q and the magnetic particles B, Can be formed in the low-density solution layer 120. [0044] FIG.

Next, as shown in FIG. 1 (c), when the magnetic force generating device 130 is brought to the lower part of the tube 100, that is, the lower part of the high density solution layer 110, The magnetic particles B are directed toward the magnetic force generating device 130. To this end, the magnetic force generator 130 must generate a magnetic force enough to apply magnetic force to the magnetic particles B that are spaced apart by the height of the low density solution layer 120.

When a magnetic force is generated by the magnetic force generating device 130 because the complex of the "quantum dot (Q) -target material (T) -magnetic particle (B)" is formed in the low density solution layer 120, (Q) -target material (T) -magnetic particle (B) "through the high-density solution layer (110) to the lower region of the tube (100).

Referring to FIG. 1 (d), the continuous magnetic force provided by the magnetic force generating device 130 causes the complex of the "quantum dot Q-target material T-magnetic particle B" And concentrated at the lower part. In the absence of the target material T, the quantum dots Q and the magnetic particles B do not form a complex. Therefore, if the target material T is absent, all the quantum dots Q remain in the low density solution layer 120 alone. On the other hand, the presence of the quantum dot Q at the bottom of the tube 100 means that the target substance T exists in the initial low density solution layer 120. If the quantum dots Q exist in the lower part of the tube 100, light of the second wavelength in the visible light region is emitted by the quantum dots Q when the first wavelengths such as ultraviolet rays are irradiated to the corresponding regions. Therefore, if the first wavelength is irradiated and light of the second wavelength emitted from the lower portion of the tube 100 is observed, it can be determined that the target substance T to be detected exists in the initial low density solution layer 120 .

In the above description, it is exemplified that one type of antibody or a substance having an affinity binds to the quantum dots Q. However, two or more quantum dots Q may be bonded to different types of antibodies or substances having affinity.

For example, to detect whether a first target material T and a second target material T are different from each other in the low-density aqueous solution 120, a first group of quantum dots Q may contain a first target material Antibody or affinity for the second target substance (T) can be bound to the quantum dots (Q) of the second group and the 'B' substance having affinity to the second target substance (T). Since the quantum dots Q emit visible light of different wavelengths with respect to the light radiated from outside according to their characteristics (for example, sizes), the quantum dots Q of the first group and the quantum dots Q of the second group have different characteristics , It can be determined whether any target material was present in the low-density aqueous solution 120. [

For example, the size of the quantum dots Q of the first group is manufactured so that visible light of the first wavelength is emitted by ultraviolet (UV) irradiation, the size of the quantum dots Q of the second group is irradiated with ultraviolet (UV) The visible light of the second wavelength can be emitted by irradiating ultraviolet (UV) light under the high density solution layer 110 in the process of FIG. 1 (d) do.

According to the present invention, whether or not the luminescence phenomenon from the quantum dot Q is detected in the lower portion of the high density solution layer 110 is confirmed by photographing the camera, and the inspection solution, that is, the initial low density solution layer 120 to determine whether the target substance (T) was present in the solution.

FIG. 2 is a view showing a configuration of a target substance analyzing apparatus using quantum dots according to an embodiment of the present invention. 3 is a side cross-sectional view showing the internal structure of a target material analyzing apparatus using quantum dots according to an embodiment of the present invention.

Referring to FIGS. 2 and 3, the target material analyzing apparatus 200 according to the embodiment may include a tube insertion port 210 and a display unit 220.

The tube insertion port 210 is a portion into which the tube 100 containing the solution to be inspected is inserted. 1) and a low-density solution layer 120 (see FIG. 1) are formed in the tube 100, and the low-density solution layer 120 is provided with a target material T), quantum dots (Q), and magnetic particles (B).

When the complex of "quantum dot (Q) -target material (T) -magnetic particle (B)" formed in the low density solution layer 120 is moved to the lower part of the high density solution layer 110 by the magnetic force, And a result of photographing light generated by the quantum point Q is displayed.

The photographed result of the light generated by the quantum point Q may be displayed as it is, but a value obtained by quantitatively quantizing it may be displayed.

The target material analyzing apparatus 200 may further include an input unit (not shown) for inputting information by a user. The user can input the kind and amount of any one of the quantum dots Q, the magnetic particles B and the target substance T added to the low density solution layer 120 of the tube 100 through the input unit. According to this, the information displayed on the display unit 220 is used to quantitatively display the amount of the target substance T currently detected based on the quantities of the added quantum dots Q, magnetic particles B and target substance T . Data processing and data generation for such quantitative display are performed by the information processing module 260 to be described later. The input unit may be separately provided, but the display unit 220 itself may be implemented as a touch screen to serve as an input unit.

Referring to FIG. 3, a magnetic force generator 230 is disposed below the tube insertion port 210. The magnetic force generating device 230 is configured to generate a magnetic field from the quantum dot Q-target material T-magnetic particles B existing in the low-density solution layer 120 located in the upper portion of the tube 100 inserted into the tube- "It works by pulling the complex using magnetic force.

The light irradiation unit 240 functions to irradiate the tube 100 inserted in the tube insertion port 210 with light of a specific wavelength. As described above, since the quantum dot Q radiates the visible light of the second wavelength when the first wavelength such as ultraviolet (UV) is irradiated, the light irradiation unit 240 irradiates the first wavelength of ultraviolet (UV) It is preferable that the module is composed of a module for

The camera module 250 is disposed at one side of the tube insertion port 210 and is disposed in the high density solution layer 110 of the tube 100 inserted into the tube insertion port 210 when light is irradiated by the light irradiation part 240 And performs a function of capturing light emitted from the quantum point Q.

(Q) -target material (T) corresponding to a certain amount by the magnetic force because the amount of the quantum dot Q attracted by the magnetic force can be detected accurately, - " magnetic particle (B) "composite is drawn.

Since the magnetic force generating device 230 is disposed below the tube insertion port 210, the cross-sectional area of the lower portion of the tube insertion port 210, that is, a portion close to the magnetic force generating device 230, is preferably narrow. If the lower portion of the tube insertion port 210 has a large cross-sectional area, the quantity of the "quantum dot Q-target material (T) -magnetic particle (B)" complex drawn by the magnetic force generating device 230 is large and small , The difference in height formed by the composite will not be large. In this case, even when the light emitted from the quantum dots Q is photographed using the camera module 250, the difference in height at which light is emitted will not be large. Therefore, it is preferable that the tube insertion port 210 is formed in a shape such that the cross-sectional area thereof becomes narrower as it approaches the magnetic force generating device 230, and the tube 100 inserted into the tube insertion port 210 is also formed with a tube insertion port 210 It is preferably formed in a corresponding shape.

3, at least the inner surface of the lowermost portion of the tube insertion port 210, that is, the inner surface of the region near the magnetic force generating device 230, , And may be formed to have a shape parallel to the plane. 3, the tube insertion port 210 has a first inner surface S1 having a first angle with respect to the longitudinal direction (the longitudinal direction in the figure) of the tube insertion port 210, And at least a portion of the second inner surface S2 may be substantially parallel to the lens of the camera module 250. In this case, The first inner surface S1 and the second inner surface S2 meet with each other with the bent portion P as a starting point.

As described above, the shape of the tube 100 has a corresponding shape so that it can be inserted into the tube insertion port 210. According to the structure shown in FIG. 3, the composite drawn by the magnetic force generating device 230 is inserted into the tube The camera module 250 can concentrate on the entire area of the second inner surface S2 and the camera module 250 can concentrate on the second inner surface S2 of the tube insertion hole 210. [ Accordingly, the imaging of the visible light emitted from the quantum dot Q and the detection of the target substance T can be performed smoothly.

3, the camera module 250 may be disposed between the tube insertion port 210 and the magnetic force generating device 230, or may be disposed in another area. That is, the camera module 250 can photograph the light emitted from the quantum dot Q existing in the high-density solution layer 110 in the tube 100 inserted into the tube insertion port 210.

On the other hand, as described above, the amount of the target material T mixed in the initial low density solution layer 120 can be grasped as the amount of the quantum point Q existing in the high density solution layer 110 after application of the magnetic force . For this reason, the camera module 250 should be able to capture only the quantum dots Q existing in the high-density solution layer 110, except for the quantum dots Q existing in the low-density solution layer 120. Since the quantum dots Q present in the low-density solution layer 120 are those that do not form a complex with the target material T. [

Therefore, in the tube 100 inserted into the tube insertion port 210, the low-density solution layer 120 must be removed before the camera module 250 captures images. For this, a process may be required to remove the low-density solution layer 120 after the tube 100 is inserted into the tube insertion port 210 and the magnetic force is sufficiently applied by the magnetic force generator 230.

After the magnetic force is sufficiently applied, the bent portion is bent so that the low density solution layer 120 is inserted into the tube insertion opening 210, So that the camera module 250 can be out of sight. That is, the high density solution layer 110 and the low density solution layer 120 are formed at the lower portion of the tube 100 and the low density solution layer 120 at the bent portion as a boundary. After the magnetic force is applied, the low density solution layer 120 exists The portion may be located outside the tube insertion port 210 or may be removed so as not to be taken by the camera module 250.

Although the camera module 250 has been described above, it is needless to say that the camera module 250 may be replaced with another device capable of measuring the amount of light emitted from the quantum point Q.

According to one embodiment, the image data photographed by the camera module 250 is transmitted to the information processing module 260. The information processing module 260 determines the amount of the quantum point Q based on the image photographed by the camera module 250. The amount of quantum dots Q to be grasped depends on the amount of quantum dots Q present in the dense solution layer 110 of the tube 100, that is, the quantum dot Q - target material (T) - magnetic particle (B) The quantity of the quantum dots Q drawn by the magnetic force generating device 230 can be determined and the quantity and quantity of the target material T can be determined on the basis of the quantum dots Q. [ The amount of quantum dots Q is determined through the image taken by the camera module 250 since the amount of quantum dots Q is the amount of the quantum dots Q-target material T-magnetic particles B complex The amount of the target substance T added to the initial low density solution layer 120 can be determined in proportion thereto.

On the other hand, when the user inputs the amount of at least one of the quantum dots Q, the target substance T and the magnetic particles B added to the low density solution layer 120 in advance, the target substance T ) Can be grasped more accurately.

For example, when the amount of quantum dots Q added to the low-density solution layer 120 is insufficient to bond with the target material T present and determined based on the image obtained by the camera module 250, The information processing module 260 can determine that the amount of the target material T is "above a specific value" if it is determined that all of the quantum dots Q previously added have been attracted to the high density solution layer 110. [

Likewise, since magnetic particles B are attracted by the magnetic force generator 230, when the amount of the magnetic particles B initially added is input, the amount of the target material T "Exceeding a specific value ", and the like.

The information determined and generated by the information processing module 260 is displayed in a form that can be confirmed by the user through the display unit 220.

Hereinafter, a method of analyzing a target material using quantum dots according to an embodiment of the present invention will be described.

4 is a flowchart illustrating a process for performing target material analysis using quantum dots according to an embodiment of the present invention.

Referring to FIGS. 1 to 4, an antibody or affinity substance capable of binding with a target substance T to be detected is attached to the quantum dots Q and the magnetic particles B (S410). Antibodies or affinity attaching substances to the quantum dots Q and the magnetic particles B can bind to the same target material T but are required to be of different kinds so as not to compete with each other. On the other hand, as described above, the quantum dots Q may be divided into two or more groups to have different characteristics, and each group may be bonded with a different kind of antibody or affinity substance.

Next, the target substance (T), the quantum dots (Q) and the magnetic particles (B) are added to the low density solution and mixed (S420). After the high density solution layer 110 is formed at the bottom of the tube 100, The low density solution is added to the upper portion to form a low density solution layer 120 in which the target material T, the quantum dot Q and the magnetic particles B are mixed (S430).

Here, the method of adding the target substance (T), the quantum dots (Q) and the magnetic particles (B) to the low density solution and then adding the mixed solution to the high density solution layer 110 is exemplified. However, The target substance (T), the quantum dots (Q), and the magnetic particles (B) may be added and mixed after the low-density solution layer 120 is formed on the substrate.

In the low-density solution layer 120, a bonding reaction occurs between the target material T and the quantum dots Q, the target material T and the magnetic particles B, and thereby the quantum dot Q - the target substance T ) -Magnetic particle (B) "complex is formed.

Thereafter, the tube 100 is inserted into the tube insertion port 210 of the target material analyzer 200, and the magnetic force applying device 230 is operated to generate a magnetic force (S440). The magnetic force application device 230 may be a device that generates magnetic force without supplying external power, but may be implemented as an apparatus that operates when a power is applied to generate a magnetic force.

After the magnetic force is sufficiently applied, the light irradiating unit 240 is operated to irradiate the first wavelength such as ultraviolet rays (UV) to the tube 100 inserted into the tube inserting hole 210 (S450). The light to be irradiated may be a light that allows the quantum point Q to react and cause a luminescence phenomenon.

After the application of the magnetic force, a process of removing the low-density solution layer 120 of the tube 100 or a process of bending a portion where the low-density solution layer 120 is formed may be performed as described above.

After the light irradiation, the light emitted by the quantum point Q is photographed through the camera module 250 (S460). The camera module 250 may be replaced with another light receiving unit or the like as described above.

The image photographed by the camera module 250 is transmitted to the information processing module 260 and analyzed (S470). The information processing module 260 determines the amount of quantum dots Q existing in the high density solution layer 110 of the tube 100 through the photographed image and thereby determines the amount of the quantum dots Q mixed in the initial low density solution layer 120 The amount of the target substance (T) can be calculated quantitatively. An application program for quantitatively calculating the amount of the quantum dots Q and quantitatively calculating the amount of the target substance T may be included in the information processing module 260 in the form of a module.

As described above, if the quantum dots Q are divided into a plurality of groups having different characteristics and combined with materials having different kinds of antibodies or affinities, images captured by the camera module 250 may have different wavelengths May be photographed.

If the quantum dots Q have different characteristics, they can emit visible light of different wavelengths by the same ultraviolet ray irradiation. Since they are bonded to substances having different kinds of antibodies or affinities, visible light having different wavelengths are detected It can be judged that different target substances T exist.

The result calculated by the information processing module 260 is transmitted to the display unit 220 and is displayed in a form that can be confirmed by the user through the display unit 220. Quantitatively calculated data can be expressed in numerical, graphical or other visual ways.

According to this embodiment, the complex of "quantum dot (Q) -target material (T) -magnetic particle (B)" is pushed into a narrow space by the magnetic force, and the target material is detected by the visible light from the quantum dot Q . Since the emission of visible light visible to the naked eye is performed by the quantum dots Q, the quantum dots Q serve to amplify signals for detecting the target substances. In addition, since the complex can be collected in a narrow space due to the role of the magnetic particles (B), the magnetic particles (B) serve to densify the target substance to be identified in a narrow space. It is possible to detect and identify the presence of a very small amount of a target substance by the quantum dots Q and the magnetic particles B. [

Meanwhile, according to the present embodiment, a sample preparation process for detecting a target material is not required.

Specifically, in a conventional method, a target substance is mixed with a substance coated with an antibody capable of binding to the target substance, and then the substance is allowed to bind to the target substance, and non-specific binding excluding the specific binding is removed Several washing steps were required. In order to measure the amount of specifically bound target substance after this washing step, a specific reaction (for example, an enzyme reaction) and amplification of the signal for the reaction were required. However, according to the embodiment of the present invention, the formation of the complex of "quantum dot (Q) -target material (T) -magnetic particle (B)" according to the specific reaction occurs in the low density solution layer, Since only the complex of the specific reaction is concentrated on the bottom of the tube, the extraction of the detection substance and the amplification of the signal can occur naturally without the sample preparation process as in the conventional method.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

Therefore, the spirit of the present invention should not be construed as being limited to the above-described embodiments, and all of the equivalents or equivalents of the claims, as well as the following claims, I will say.

100: tube
110: non-aqueous solution layer
120: aqueous solution layer
200: Target material analyzer
210: tube insertion port
220:
230: magnetic force generating device
240:
250: Camera module
260: Information processing module

Claims (10)

An apparatus for analyzing a target material using quantum dots,
A tube insertion port into which a tube having a high density solution layer at the bottom and a low density solution layer in which a target substance, a quantum dot, and magnetic particles are mixed is formed;
A magnetic force generating device formed at a lower portion of the tube inserting port to cause a composite of the target substance, quantum dot, and magnetic particles bound in the low density solution layer to be led to the lower portion of the tube through the high density solution layer; And
And a camera module for detecting light emitted by quantum dots of said complex present in said dense solution layer in response to a particular wavelength. The method according to claim 1,
Further comprising an information processing module for determining the presence or absence of the target material constituting the complex together with the quantum dots according to the presence or amount of quantum dots determined based on the light detection result by the camera module, Device. 3. The method of claim 2,
And a display unit for displaying information on the quantum dots or the target material as judged by the information processing module. The method according to claim 1,
Further comprising a light irradiating section for applying the specific wavelength to the tube. The method according to claim 1,
Wherein the tube insertion port is formed to have a narrow cross-sectional area as it approaches the magnetic force generating device. A method for analyzing a target material using a quantum dot,
Attaching a substance having an antibody or affinity to a specific target substance to the quantum dots and magnetic particles;
Forming a high-density solution layer on the lower part of the tube, forming a low-density solution layer in which the quantum dots, the magnetic particles, and the target material are mixed on the upper part, and attaching the quantum dots and the magnetic particles, To form a complex with the target material;
Applying a magnetic force to the lower portion of the tube so that the composite is led to the lower portion of the tube through the dense solution layer; And
Determining whether a quantum dot that emits light in response to the specific wavelength is present in the dense solution layer after applying a specific wavelength to the tube. The method according to claim 6,
Wherein the attaching comprises:
Attaching an antibody or affinity substance to the first target substance to the quantum dot having the first characteristic and attaching the antibody or affinity substance to the second target substance to the quantum dot having the second characteristic. Target material analysis method. The method according to claim 6,
Further comprising the step of determining the presence or amount of the target substance based on the amount of quantum dots present in the dense solution layer. The method according to claim 6,
After the magnetic force applying step,
Further comprising the step of removing said low-density solution layer. The method according to claim 6,
The step of determining whether or not the quantum dot is present comprises:
Capturing the light emitted from the quantum dots after applying the specific wavelength.
KR1020140034866A 2014-03-25 2014-03-25 Apparatus and method for detecting target material using quantum dot KR101405053B1 (en)

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