KR101401997B1 - Composition for removing chromaticity of dye wastewater and method for removing chromaticity of dye wastewater - Google Patents

Composition for removing chromaticity of dye wastewater and method for removing chromaticity of dye wastewater Download PDF

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KR101401997B1
KR101401997B1 KR20120017886A KR20120017886A KR101401997B1 KR 101401997 B1 KR101401997 B1 KR 101401997B1 KR 20120017886 A KR20120017886 A KR 20120017886A KR 20120017886 A KR20120017886 A KR 20120017886A KR 101401997 B1 KR101401997 B1 KR 101401997B1
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kuc9065
dyeing wastewater
chromaticity
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김규혁
김재진
최용석
서자연
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고려대학교 산학협력단
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Abstract

The present invention relates to a method for removing the color of the color removal composition and a dyeing waste water for the dyeing waste water, more specifically, suberic compartment Temple Adu star KUC9065 (Bjerkandera adusta KUC9065) (Accession No .: KACC93146P), and a method for removing the chromaticity of the dyeing wastewater used.

Figure R1020120017886

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for removing chromaticity of a dyeing wastewater and a method for removing chromaticity of dyeing wastewater,

The present invention relates to a composition for removing coloring of dyeing wastewater and a method for removing coloring of dyeing wastewater.

When dyeing wastewater containing colored dyes generated in the dyeing process is released into the water system, it may reduce the light transmittance of the water system, which can threaten the survival of aquatic life products that require sunlight. Therefore, the dyeing wastewater should be pretreated to remove chromaticity before the water system is discharged.

Currently, much research has been carried out on the basis of physical or chemical methods to remove the chromaticity of dyeing wastewater. However, color removal of dyeing wastewater by physical and chemical methods is costly and may lead to secondary environmental pollution by the chemicals used.

Recently, a method for removing chromaticity of dyeing wastewater using microbial metabolism has been studied to replace the physical and chemical methods. Among them, the biological method of removing chromaticity using white rot fungi is lignin secreted by white rot fungi It has been reported that the degrading enzyme is suitable for removing chromaticity of dyeing wastewater because it can decompose not only lignin but also various dyes having similar chemical structure to lignin.

Korean Patent Laid-Open Publication No. 2009-0106689 discloses a method of attaching a white rot fungi to a polyurethane foam carrier containing an organic sludge carbide and applying the same to a floating media biofilm reactor to remove chromaticity and the like. However, among the white rot fungi, Phanerochate Chysosporium (KCTC 6147) is only described, but the strains exhibiting high chromaticity removal efficiency among white rot fungi are not described.

In addition, the most basic consideration in the color removal technique of dyeing wastewater using white rot fungi is the type of strain. The chromaticity reduction study of dyeing wastewater using white rot fungi was conducted on several excellent strains reported in the past . Therefore, the efficiency of chromaticity removal is limited. Especially, some well-known strains in Korea are used for research in the overseas strain bank. Therefore, when used in the dyeing wastewater treatment process in Korea, it causes disturbance of domestic ecosystem by introduction of foreign species .

In addition, studies on the chromaticity of dyeing wastewater using white rot fungi generally focus on the possibility of removing chromaticity of dyes in laboratory scale. However, in dyeing wastewater discharged from actual dyeing factories, salt, surfactant, metal, toxic Organic compounds, fungicides, and toxic anions. Therefore, the possibility of dye chromaticity removal in a simple laboratory is limited when the actual dyeing waste water color removal of the strain is applied.

Korea Patent Publication No. 2009-0206689

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems of the prior art, and it is an object of the present invention to provide a white rot fungus which is excellent in removing chromaticity contained in dyeing wastewater, comprising Bjerkandera adusta KUC9065 (accession number: KACC93146P) And to provide a composition.

A further object of the present invention is to provide a method for the production of said white rot fungus, Berkerkandera KUC9065 adusta KUC9065) (accession number: KACC93146P). The present invention provides a method for removing chromaticity contained in dyeing wastewater.

In order to attain the above object, the present invention provides a composition for removing color of a dyeing wastewater comprising Bjerkandera adusta KUC9065 (accession number: KACC93146P).

In addition, the present invention relates to a method for the treatment of Berkandra < RTI ID = 0.0 > adusta KUC9065) (Accession No .: KACC93146P).

The inventive Verkandera agusta KUC9065 ( Bjerkandera adusta KUC9065) (Accession No .: KACC93146P) can provide a composition suitable for the chromatic removal of dyeing wastewater discharged from an actual dyeing plant. In addition, as a white rot fungus, Berkandera Adusta KUC9065 ( Bjerkandera adusta KUC9065) (Accession No .: KACC93146P) can be substituted for existing physical and chemical processes by applying it to the actual dyeing waste water color removal process.

In addition, the problem of disturbance of the ecosystem can be solved through the composition for removing the color of the dyeing wastewater using the domestic species among the white rot fungi and the method for removing the chromaticity of the dyeing wastewater.

Fig. 1 is a graph comparing the absorbances of Example 1, Comparative Examples 1 to 3 and the raw wastewater.
Fig. 2 is a graph comparing the absorbances of Example 1, Comparative Examples 1 to 3, and wastewater stock solution.
3 is a graph showing the chromaticity removal rate of the dyeing wastewater of Example 1. Fig.
4 is a graph showing the difference between the dyeing wastewater of Example 1 before and after color removal.

Hereinafter, the present invention will be described in detail.

The present invention relates to a process for the preparation of Bjerkandera < RTI ID = 0.0 > adusta KUC9065) (accession number: KACC93146P).

White Rot Fungi is a wood rot fungus that breaks down the material that makes up the wood. It decomposes the complicated structure of lignin that keeps the wood firmly among the constituents of the wood, so the wood rotates and turns white. It is called dead bacteria. At this time, the lignin-decomposing enzyme secreted by the white rot fungus can decompose not only lignin but also various dyes having a chemical structure similar to that of lignin, so that it is suitable for removing chromaticity of dyeing wastewater.

One of the above-mentioned white rot fungi was the Berkandera adusta KUC9065) is deposited at the National Institute of Agricultural Science and Technology (KACC93146P), and is excellent in removing chromaticity contained in dyeing wastewater generated from dyeing factories. Specifically, it is useful for removing chromaticity contained in actual wastewater including heavy metals as well as chromaticity removal by laboratory level reagents.

Thus, the Berkandera KUC9065 adusta KUC9065) (Accession No .: KACC93146P) can be applied to a biological dyeing wastewater treatment process that replaces existing physical and chemical processes.

At this time, the dyeing wastewater may include a heavy metal, and the heavy metal is preferably at least one heavy metal selected from the group consisting of cadmium, chromium, copper, lead and arsenic, but is not limited thereto.

The present invention relates to a process for the preparation of Bjerkandera < RTI ID = 0.0 > adusta KUC9065) (Accession No .: KACC93146P). Specifically, Berkandera < RTI ID = 0.0 > KUC9065 adusta KUC9065) (accession number: KACC93146P), to prepare a culture medium, and a step of culturing the culture medium with the above-mentioned BUCKANDA adusta KUC9065) (Accession No .: KACC93146P) in a medium. At this time, it is preferable that the culturing step is carried out by shaking culture at 25 to 30 DEG C and 120 to 180 rpm for 10 days or more, but it is not limited thereto.

Berkandera Adusta KUC9065 ( Bjerkandera (mm / day) of 2% malt extract agar (MEA) medium temperature at 20 ° C was 6.92, 6.49 at 25 ° C, 7.13 at 30 ° C and 4.08 at 35 ° C ( adusta KUC9065) (Accession No .: KACC93146P) The growth inhibition can be reduced when cultivated under the conditions of 25 캜 to 30 캜. In addition, under shaking culture conditions of less than 120 rpm, the supply of oxygen to the culture medium may be restricted, and conditions for shaking culture in excess of 180 rpm are Bjerkandera adusta KUC9065) (Accession No .: KACC93146P). In addition, the Berkandera Adusta KUC9065 ( Bjerkandera adusta KUC9065) (Accession No .: KACC93146P) is preferably cultured for at least 10 days in order to sufficiently remove the color of the dyeing wastewater.

At this time, the dyeing wastewater may include a heavy metal, and the heavy metal is preferably at least one heavy metal selected from the group consisting of cadmium, chromium, copper, lead and arsenic, but is not limited thereto.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Reference Example  One: In solid medium  Dye bleaching>

A total of 221 white rot fungi isolated and collected from various host plants in Korea were tested for discoloration ability of three kinds of synthetic dyes congo red (CR), orange-Ⅱ (O-Ⅱ) and reactive blue-4 (RB-4) Respectively. In order to evaluate the decolorization ability, 221 malt extracts were inoculated at the center of a 2% malt extract agar (MEA) medium containing 100 mg / L of CR, O-Ⅱ and RB- And cultured at 27 DEG C for 6 days.

The white rot fungi which had the decolorizing ability of the dyestuff among the test strains grew in a circular shape around the center of the medium in which the mycelium was inoculated with the lapse of the culture period, and the unique color of each dye was removed. After the completion of the culture, the diameter of the portion where the unique color of the dye was removed was measured and compared with the total diameter (90 mm) of the medium used to evaluate the dye decoloring ability.

As a result, as shown in Table 1, 25 white rot fungi having an average decolorization rate of 70% or more were selected for the three kinds of dyes.

Fungal isolate KUC No. Congo red Orange II Reactive
blue 4 Average Removal Rate (%) Phanerochaete calotricha KUC8003 ++ ++++ +++++ 70 Phanerochaete sordida KUC8032 ++++ +++++ +++++ 92 Ceriporia lacerata KUC8090 ++++ +++++ +++++ 95 Ceriporia lacerata KUC8138 ++++ +++++ +++++ 95 Ceriporia lacerata KUC8139 ++++ ++++ ++++ 88 Bjerkandera adusta KUC8204 ++ ++++ ++++ 71 Phanerochaete velutina KUC8301 ++ +++++ +++++ 78 Phanerochaete sp.1 KUC8370 ++++ +++++ +++++ 92 Phanerochaete sordida Ⅰ KUC8405 ++ +++++ +++++ 78 Phlebia radiata KUC8406 ++ +++++ +++++ 77 Irpex lacteus KUC8508 ++++ ++++ +++++ 80 Porostereum spadiceum KUC8602 ++ +++++ +++++ 77 Ceriporia lacerata KUC8614 +++ ++++ ++++ 78 Phanerochaete velutina KUC8727 ++ +++++ +++++ 79 Perenniporia fraxinea KUC8728 ++++ ++++ +++++ 86 Phanerochaete velutina KUC8801 ++ +++++ +++++ 77 Bjerkandera adusta KUC8808 ++ +++++ +++++ 78 Ceriporia lacerata KUC8814 +++++ +++++ +++++ 97 Phanerochaete velutina KUC8815 ++ +++++ +++++ 80 Irpex lacteus KUC9013 ++++ +++ +++++ 85 Irpex lacteus KUC9014 ++++ +++ ++++ 76 Phanerochaete aculeata KUC9032 ++ +++++ ++++ 72 Bjerkandera adusta KUC9065 +++ ++++ ++++ 76 Bjerkandera adusta KUC9107 ++ ++++ ++++ 74 Phanerochaete sordida KUC9201 ++++ +++++ +++++ 91

< Reference Example  2: In a liquid medium  Dye discoloration Outstanding strain  Selection>

The bleaching ability of the liquid dye was evaluated on the above 25 white rot fungi. First, 100 mg / L of three kinds of synthetic dyes CR, O-Ⅱ and RB-4 were added to a 250 mL Erlenmeyer flask containing 100 mL of 2% malt extract (ME) solution and sterilized at 121 캜 for 15 minutes Liquid medium was prepared. The strains pre-cultured in a 2% malt extract agar (MEA) medium were inoculated into a prepared liquid medium at a size of 5 mm in diameter and cultured at 27 ° C and 150 rpm for 10 days with shaking. After completion of the culture, 2 ml of the culture was collected and used as a sample for evaluation of decolorization ability after removing mycelia using a 0.45 μm syringe filter.

The decolorization of the sample was measured by using a spectrophotometer. The absorbance of the sample was measured at the wavelength at which the maximum absorbance of each dye was measured, and the decolorization degree was compared with the absorbance of the liquid medium to which the strain was not inoculated. In order to select the strains among them, strains with high chromaticity removal rate in the medium containing all of CR, O-Ⅱ and RB-4 were used as the strains of Ceriporia lacerata KUC8090) (Accession No .: KACC93147P), Verankendraadusta KUC9065 ( Bjerkandera (Accession No .: KACC93145P), adusta KUC9065 (Accession No .: KACC93146P), Phanerochaete calotricha KUC8003 (Accession No .: KACC93144P) and Porostereum spadiceum KUC8602 (Accession No .: KACC93145P) Respectively.

Fungal isolate KUC No. Congo
red Orange
II Reactive blue 4 Three
dyes
mix Ceriporia lacerata KUC8090 89.8 99.0 94.9 94.2 Bjerkandera adusta KUC9065 92.0 97.4 97.7 93.8 Ceriporia lacerata KUC8814 87.9 98.4 92.0 93.6 Ceriporia lacerata KUC8614 84.8 98.3 84.8 92.8 Ceriporia lacerata KUC8138 88.9 99.0 92.8 92.7 Ceriporia lacerata KUC8139 88.6 98.9 93.2 90.6 Phanerochaete calotricha KUC8003 64.0 61.7 90.8 87.3 Bjerkandera adusta KUC9107 91.8 93.0 96.5 83.0 Porostereum spadiceum KUC8602 87.7 99.0 97.8 80.1 Bjerkandera adusta KUC8808 90.8 92.1 97.0 76.0 Phlebia radiata KUC8406 55.9  0.5 42.5 36.9 Perenniporia fraxinea KUC8728 48.7 -0.2 26.2 27.5 Irpex lacteus KUC8508 78.4 13.6 86.1 27.0 Phanerochaete velutina KUC8301 39.1 -4.3 -4.9 25.9 Phanerochaete velutina KUC8801 48.0 -4.8 -2.7 23.9 Irpex lacteus KUC9014 73.5 30.9 85.2 22.8 Phanerochaete sordida KUC9201 36.9 -6.1 -5.4 19.3 Phanerochaete sordida KUC8032 44.2 24.5 37.4 18.2 Phanerochaete velutina KUC8727 40.2 -4.2  5.7 17.8 Phanerochaete sordida Ⅰ KUC8405 38.2 -3.5 17.6 16.8 Phanerochaete velutina KUC8815 44.5 -3.0  2.3 15.4 Phanerochaete aculeata KUC9032 32.6 46.4 97.4 12.6 Irpex lacteus KUC9013 64.3 -2.5 58.5  4.8 Bjerkandera adusta KUC8204 75.3 -3.7 49.6  4.3 Phanerochaete sp .One KUC8370 46.7  6.3 90.2  0.9

< Example  One: Vercandera Adusta  KUC9065 ( Bjerkandera adusta KUC9065 )>

Berkerkandera KUC9065 deposited at the National Institute of Agricultural Science and Technology Center of Agricultural Genetic Resources ( Bjerkandera adusta KUC9065) (Accession No .: KACC93146P) was evaluated for its ability to remove color from the dyeing wastewater discharged from the actual dyeing plant. The dyeing wastewater is dyeing wastewater taken from the cooperative association of dyeing business of Gyeonggi-do, and the composition and characteristics thereof are shown in Table 3 below.

The dyeing wastewater containing Basal-salts medium was inoculated with the above-mentioned Bjerkandera adusta KUC9065 (accession number: KACC93146P) with a diameter of 5 mm and cultured at 27 ° C and 150 rpm for 2 weeks with shaking Respectively. After completion of the culture, the culture solution was collected and used as a sample for evaluation of decolorization ability after removing mycelia using a 0.45 μm syringe filter.

pH 9.03 Dissolved oxygen amount (D.O.) 1.63 mg / L Salinity 0.097% Conductivity 1.359ms / cm Total dissolved solids (TDS) 1.234 g / L Heavy metal concentration CD 0.12 ppm Cr 0.05 ppm Cu 0.03 ppm Pb 0.12 ppm As 0.05 ppm

< Comparative Example  One: Three Li Pori La Serra  KUC8090 ( Ceriporia lacerata KUC8090 )>

In Example 1, a white rot fungus was used as Verankendraadusta KUC9065 (Bjerkandera adusta KUC9065), which was deposited at the National Institute of Agricultural Science and Technology,Ceriporia lacerata KUC8090) (Accession No .: KACC93147P) was cultured in the same manner as in Example 1. [

< Comparative Example  2: Paneroccaete Kalotrica  KUC8003 ( Phanerochaete calotricha KUC8003 )>

In Example 1, the white rot fungus was Berkandera KUC9065 adusta KUC9065), instead of the fungicide KAC8003 ( Phanerochaete calotricha KUC8003) (Accession No .: KACC93144P) were cultured.

< Comparative Example  3: Captosterium Spadesize  KUC8602 ( Porostereum spadiceum KUC8602 )>

In Example 1, the white rot fungus was Berkandera KUC9065 adusta KUC9065), instead of the wild-type strain KUC8602 ( Porostereum spadiceum KUC8602) (Accession No .: KACC93145P) were cultured in the same manner as in Example 1.

< Experimental Example  1: Evaluation of discoloration of dyeing wastewater>

The color removal ability of the dyeing wastewater discharged from the actual dyeing plant was evaluated for Example 1 and Comparative Examples 1 to 3.

The decolorization of the sample was measured by using a spectrophotometer. The absorbance of the sample was measured at the wavelength range of visible light (380 ~ 750nm), and the absorbance difference between the raw wastewater was compared. As a result, as shown in Tables 4 and 5 and Figs. 1 and 2, the Berkandera KUC9065 of Example 1 adusta KUC9065) (Accession No .: KACC93146P) exhibited the most excellent color removal ability of the dyeing wastewater. That is, due to the influence of the heavy metals contained in the wastewater, it is necessary to treat the actual wastewater by using Berkerkandera KUC9065 adusta KUC9065) (Accession No .: KACC93146P) is the most efficient.

Absorbance (Abs) Example 1 (Vercandera agusta KUC9065) COMPARATIVE EXAMPLE 1 (SEPRIORIA LA SERERATA KUC8090) Comparative Example 2 (Panerocca eticotrica KUC8003) Comparative Example 3 (Porosterium sp. Raw wastewater Purple 0.094 0.189 0.156 0.185 0.257 blue 0.044 0.120 0.096 0.110 0.173 Cyan 0.032 0.104 0.084 0.091 0.152 green 0.020 0.087 0.073 0.069 0.135 yellow 0.013 0.076 0.066 0.055 0.123 Orange 0.011 0.073 0.064 0.050 0.110 Red 0.009 0.069 0.061 0.039 0.065

Chromaticity Removal Rate (%) Example 1 (Vercandera agusta KUC9065) COMPARATIVE EXAMPLE 1 (SEPRIORIA LA SERERATA KUC8090) Comparative Example 2 (Panerocaetecalotrica KUC8003) Comparative Example 3 (Porosterium sp. Purple 63.3 26.5 39.3 28.0 blue 74.7 30.4 44.3 36.5 Cyan 78.9 31.4 44.5 40.2 green 84.9 35.4 45.7 48.5 yellow 89.6 38.4 46.2 55.0 Orange 90.3 33.5 41.6 54.5 Red 86.2 -5.0 7.2 40.2

National Institute of Agricultural Science KACC93144P 20120216 National Institute of Agricultural Science KACC93145P 20120216 National Institute of Agricultural Science KACC93146P 20120216 National Institute of Agricultural Science KACC93147P 20120216

Claims (7)

Includes Bjerkandera adusta KUC9065 (Accession No: KACC93146P)
Wherein said Vercandera agusta KUC9065 decomposes one or more of the purple, blue, cyan, green, yellow, orange, or red dyes present in the dyeing wastewater.
A composition for removing the color of dyeing wastewater. The composition of claim 1, wherein the dyeing wastewater comprises heavy metals. The composition according to claim 2, wherein the heavy metal is at least one heavy metal selected from the group consisting of cadmium, chromium, copper, lead and arsenic. Using the Bjerkandera adusta KUC9065 (Accession No: KACC93146P)
Wherein said Vercandera agusta KUC9065 decomposes one or more of the purple, blue, cyan, green, yellow, orange, or red dyes present in the dyeing wastewater.
A method for removing chromaticity of dyeing wastewater. The method of claim 4, wherein the step of removing the chromaticity of the dyeing wastewater comprises the steps of: preparing a medium containing dyeing wastewater, and adding to the medium the Berkerkandera KUC9065 adusta KUC9065) (Accession No .: KACC93146P). &lt; / RTI &gt; 5. The method of claim 4, wherein the dyeing wastewater comprises heavy metals. 7. The method of claim 6, wherein the heavy metal is at least one heavy metal selected from the group consisting of cadmium, chromium, copper, lead, and arsenic.
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Cited By (2)

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KR20170119929A (en) * 2016-04-20 2017-10-30 이화여자대학교 산학협력단 Novel dye-decolorizing fungus, Bjerkandera adusta NIBRKDFPFGC000002676, and method for treating dye wastewaters using the same
KR102327435B1 (en) 2020-08-20 2021-11-17 주식회사 마이셀랩 Culture broth of Porostereum sp.(KCTC18837P) and Cosmetic composition comprising the same for improving skin condition

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CN104173387B (en) * 2014-08-25 2018-01-16 四川省中医药科学院 The pharmaceutical usage of clarinet pore fungi and the pharmaceutical composition for treating tumour
KR101942476B1 (en) * 2016-04-20 2019-01-28 이화여자대학교 산학협력단 Novel dye-decolorizing fungus, Trametes versicolor NIBRKDFPFGC000002677, and method for treating dye wastewaters using the same
CN113621527B (en) * 2021-09-27 2022-03-25 阜阳师范大学 Novel smoke tube bacterium SZ-4 and application thereof

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Publication number Priority date Publication date Assignee Title
KR20170119929A (en) * 2016-04-20 2017-10-30 이화여자대학교 산학협력단 Novel dye-decolorizing fungus, Bjerkandera adusta NIBRKDFPFGC000002676, and method for treating dye wastewaters using the same
KR102327435B1 (en) 2020-08-20 2021-11-17 주식회사 마이셀랩 Culture broth of Porostereum sp.(KCTC18837P) and Cosmetic composition comprising the same for improving skin condition

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