KR101397145B1 - Method for obtaining extract of raw ginseng and raw ginseng at the same time - Google Patents

Abstract

The present invention relates to a method of obtaining a liquid wild ginseng extract having the increased active component content and a wild ginseng having the improved storing properties to be stored for a long time at the same time. The method for obtaining a liquid wild ginseng extract and a wild ginseng at the same time according to the present invention comprises the steps of: pressing a wild ginseng vacuum packed with water by 200-400 Mpa for 7-13 minutes; pressing the treated wild ginseng by 0.05-1.50 Mpa for 3-7 minutes to obtain a wild ginseng processed product; and separating a liquid wild ginseng extract and the wild ginseng from the wild ginseng processed product.

Description

METHOD FOR OBTAINING EXTRACT OF RAW GINSENG AND RAW GINSENG AT THE SAME TIME BACKGROUND OF THE INVENTION 1. Field of the Invention < RTI ID = 0.0 >

The present invention relates to a method for simultaneously obtaining an aqueous solution of a ginseng extract having an increased active ingredient content and a ginseng ginseng having improved storage properties for a long period of storage.

Conventionally, there has been a method of extracting the ginseng ginseng and then increasing the extraction efficiency of the ginseng ginseng by enzymatic treatment and heat treatment. In this case, the component of the ginseng ginseng itself is changed by hydrolyzing the ginseng ginseng or by treating the enzyme with the ginseng ginseng There was a disadvantage. Even in the case of treating the goat wild ginseng by heat treatment or the like, the red ginseng can not necessarily be produced according to the above-mentioned process, so that the ginseng itself can not be obtained as a raw material.

Korean Patent Publication No. 10-2009-0086806

It is an object of the present invention to provide a method for simultaneously obtaining an extract liquid phase and an organic ginseng raw material which are processed to prepare a high-dose active ingredient by processing the ginseng ginseng.

According to an aspect of the present invention, there is provided a method for preparing a ginseng saponified ginseng product, comprising the steps of: (A) treating ginseng saponified with water in vacuum at 200 to 400 MPa; (B) treating the goat ginseng under a pressure of 0.05 to 1.5 MPa to obtain a goat ginseng product; And (C) separating the ginsenosides extract liquid phase and the ginsenoside ginsenoside from the ginseng ginseng process product. The present invention also provides a method for simultaneously obtaining the ginseng extract liquid phase and the ginseng ginseng product.

One aspect of the present invention is also (1) A method for producing a ginseng saponin, comprising the steps of: (1) treating ginseng, which has been vacuum-packed with water, at a pressure of 200 to 400 MPa for 7 to 13 minutes; (2) treating the goat ginseng at a pressure of 0.05 to 1.5 MPa for 3 minutes to 7 minutes to obtain a goat ginseng processed product; (3) separating only the ginseng from the ginseng root ginseng product; And (4) vacuum packing the separated ginseng ginseng, wherein the method comprises repeating the step (1) and the step (2) two to four times To provide a method for increasing the shelf life of the goat wild ginseng.

According to the present invention, it is useful to simultaneously obtain the raw goat's ginseng extract liquid and the raw ginseng ginseng in the raw form. In addition, the extraction yield of the goat ginseng liquid phase increased by the repetition of the ultrahigh pressure and low pressure treatment, and the content of the fragrance component and saponin as the active ingredients in the liquid phase increased. In addition, the activity of the goat ginseng in the raw material form can be maintained even when it is stored for a long period of time, so that storage and storage properties are excellent.

Fig. 1 shows the crude saponin content of the extract of the present invention of the present invention when compared with the control group.
FIG. 2 shows the content of the fragrance component of the extract of the present invention of the present invention compared with the control group.
FIG. 3 is a photograph for visual evaluation that the vacuum packed goat ginseng of the present invention is retained even after prolonged time. (LW: atmospheric pressure extraction; HW: ultra-high pressure extraction)

In one aspect, the present invention provides a process for preparing a ginseng product, comprising: (A) treating ginseng, which has been vacuum-packed with water, under pressure of 200 to 400 MPa; (B) treating the goat ginseng under a pressure of 0.05 to 1.5 MPa to obtain a goat ginseng product; And (C) separating the ginsenosides extract liquid phase and the ginsenoside ginsenosides from the ginseng ginseng product. The present invention also relates to a method for simultaneously obtaining the ginseng extract liquid phase and the ginseng ginseng.

In the present specification, the 'vacuum packaging' can be applied to any packing method for packing a material capable of withstanding high pressure by a vacuum packaging method used in the related art. The vacuum packaging can be applied to a plant or animal (meat) Any packaging method can be used without limitation. Specifically, the vacuum package may be packed with a polyethylene material.

In the method for simultaneously obtaining the active ingredient-added ginseng extract liquid phase and the ginseng ginseng obtained from the aspect of the present invention, the ginseng ginseng can be vacuum-packed together with water. As the raw ginseng is treated with water and subjected to ultrahigh pressure processing, the active ingredient of the ginseng ginseng easily escapes into water and the content of the active ingredient in the ginseng ginseng extract liquid can be improved.

The treatment of the fresh ginseng at the pressure range of 200 to 400 MPa may increase the content of the active ingredient in the extract of the ginseng extract and may increase the content of the fragrance ingredient specific to the ginseng ginseng and is useful for long-term storage. In view of the above, in the step (A) of the method which is an aspect of the present invention, the pressure range is from 210 to 390 MPa, from 220 to 380 MPa, from 230 to 370 MPa, from 240 to 360 MPa, 260 to 340 MPa.

The step (B) of treating the ginseng root at a pressure of 0.05 to 1.5 MPa may increase the content of the active ingredient in the ginseng extract of the ginseng and may increase the amount of the ginseng component specific to the ginseng root, . In view of the above, in step (A) of the method which is an aspect of the present invention, the pressure range is from 0.055 to 1.45 MPa, from 0.06 to 1.4 MPa, from 0.065 to 1.35 MPa, from 0.07 to 1.3 MPa, 0.07 to 1.2 MPa.

In the method for simultaneously obtaining the aqueous solution of the extract of the goat's ginseng extract and the ginseng of the ginseng, which is an aspect of the present invention, the step (A) is treated for 7 to 13 minutes, the step (B) is treated for 3 to 7 minutes can do. Due to the above-mentioned time processing, the content of the active ingredient in the extract of the goat ginseng extract may be increased, the content of the seaweed ingredient specific to the ginseng ginseng may be increased, and is useful for long-term storage. From this point of view, the step (A) can be treated for 8 to 12 minutes or the treatment for 9 to 11 minutes, and the step (B) can be treated for 4 to 6 minutes.

In the method for simultaneously obtaining the active ingredient-rich goat's ginseng extract liquid and the ginseng ginseng, which is an aspect of the present invention, the method comprises repeating steps (A) and (B) two to four times, C). ≪ / RTI > Through the process of repeating the above-mentioned high pressure and low pressure treatment process, the content of the active ingredient in the extract of the goat ginseng extract may be increased, the content of the seaweed ingredient specific to the ginseng ginseng may be increased, and is useful for long-term storage . In view of the above, the processing of step (A) and step (B) may be repeated 2.5 to 3.5 times. In the method of simultaneously obtaining the active ingredient-derived ginseng extract of the present invention and the ginseng extract of the present invention, which is an aspect of the present invention, the repeated number of times is determined by repeating steps (A) and (B) , And repeating the operation at each number of times.

In one aspect of the present invention, there is provided a method for simultaneously obtaining an active ingredient-rich goat's ginseng extract liquid phase and a ginseng ginseng in an aspect of the present invention, the method further comprising the step of (B ') leaving the ginseng extract solution at 2-8 ° C . Through the process of treating under the above temperature, the content of the active ingredient in the extract of the ginseng extract may be increased, the content of the ginseng ingredient specific to the ginseng ginseng may be increased, and is useful for long-term storage. In view of the above, the method may further comprise the step of (B ') leaving the goat's ginseng extract liquid phase at 3 to 7 ° C or 4 to 6 ° C.

In the method of simultaneously obtaining the active ingredient-derived ginseng extract of the present invention and the ginseng extract of the present invention as an aspect of the present invention, the extract of the ginseng extract of step (B ') may be left for 1 to 3 days. Through the process of aging the ginseng extract, the content of the ginseng extract specific to ginseng can be increased. From the above point of view, the goat's ginseng extract liquid phase can be left for 2 days.

In the present specification, the 'vacuum packaging' can be applied to any packing method for packing a material capable of withstanding high pressure by a vacuum packaging method used in the related art. The vacuum packaging can be applied to a plant or animal (meat) Any packaging method can be used without limitation. Specifically, the vacuum package may be packed with a polyethylene material.

In the method of simultaneously obtaining the active ingredient-added ginseng extract of the present invention, which is one aspect of the present invention, the ginseng ginseng may be a ginseng ginseng grown by implanting a seedling or a seedling planted with an acid sprayed on an acid . In the present specification, the 'ginseng ginseng' can be applied to any kind of ginseng, and specifically, 7 to 12 year old ginseng ginseng can be used, but is not limited thereto.

In another aspect, the present invention provides a process for preparing a ginseng saponin, comprising the steps of: (1) treating ginseng, which has been vacuum-packed with water, at a pressure of 200 to 400 MPa for 7 to 13 minutes; (2) treating the goat ginseng at a pressure of 0.05 to 1.5 MPa for 3 minutes to 7 minutes to obtain a goat ginseng processed product; (3) separating only the ginsenoside from the ginseng ginseng product, and (4) vacuum packing the separated ginseng ginseng, wherein the method comprises the steps of: (1) And (2) repeating the treatment two to four times.

It is useful to treat goat's ginseng ginseng in the pressure range of 200 to 400 MPa for long-term storage of ginseng ginseng after treatment. In view of the above, in the step (A) of the method which is an aspect of the present invention, the pressure range is from 210 to 390 MPa, from 220 to 380 MPa, from 230 to 370 MPa, from 240 to 360 MPa, 260 to 340 MPa.

The step (2) of treating the above-mentioned goat ginseng under a pressure of 0.05 to 1.5 MPa is useful for long-term storage of ginseng. In view of the above, in step (A) of the method which is an aspect of the present invention, the pressure range is from 0.055 to 1.45 MPa, from 0.06 to 1.4 MPa, from 0.065 to 1.35 MPa, from 0.07 to 1.3 MPa, 0.07 to 1.2 MPa.

In the method for increasing the shelf life of the goat ginseng as one aspect of the present invention, the step (1) may be treated for 7 to 13 minutes, and the step (2) may be treated for 3 to 7 minutes. Due to the above-mentioned time processing, the content of the active ingredient in the extract of the goat ginseng extract may be increased, the content of the seaweed ingredient specific to the ginseng ginseng may be increased, and is useful for long-term storage. From this point of view, the step (1) can be treated for 8 to 12 minutes or the treatment for 9 to 11 minutes, and the step (2) can be treated for 4 to 6 minutes.

In one aspect of the present invention there is provided a method for increasing the shelf life of a goat ginseng, wherein the ginseng comprises all kinds of goat ginseng from 1 to 20 years of age.

In another aspect, the present invention relates to a method for the preparation of a compound of formula (I), which is selected from the group consisting of? -Panasinsene, Calarene,? -Humulene, (+) - spathulenol The present invention relates to an extract liquid phase of a high temperature treated goat ginseng comprising at least one compound selected from the group consisting of ethyl phthalate and isopathulenol.

In another aspect, the present invention relates to a method for preparing a ginseng saponin, comprising the steps of: (1) treating ginseng roots germinated in vacuum with water under a pressure of 200 to 400 MPa for 7 to 13 minutes; (2) treating the goat ginseng at a pressure of 0.05 to 1.5 MPa for 3 minutes to 7 minutes to obtain a goat ginseng processed product; (3) separating only the ginsenoside ginseng among the ginseng ginseng products, and (4) vacuum-packing the separated ginseng ginseng.

Hereinafter, the present invention will be described more specifically with reference to the following examples. However, the following examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

 [ Example  One] Goat ginseng  Production of extractive liquid phase

6-year-old goats 20 g of ginseng roots and 50 mL of water were packed together in a special pack for ultrahigh-pressure treatment, sealed in a special pack to prevent air bubbles, and then put into an ultra-high pressure reactor and subjected to ultrahigh pressure treatment at 300 MPa. The ultrahigh pressure treatment was carried out for 10 minutes at 300 MPa and then for 5 minutes at 0.1 MPa for 3 times. From these extracts, the ginseng ginseng was removed and only the liquid form of the extract liquid was isolated.

As a control, samples (0.1 MPa) without ultra-high pressure treatment were extracted at room temperature (28 ° C) for 45 minutes.

The extracted liquid phase of Example 1 of the present invention and the control group were aged at 5 ° C for 0, 1, 7, and 14 days, respectively.

[ Experimental Example  1] Yield comparison

The degree of dissolution was compared between the extract liquid of Example 1 of the present invention and the control sample. 10 mL of each sample was taken out and filtered with a cotton wool pad. The filtrate was concentrated under reduced pressure, and the dried content of the extract was weighed and shown in Table 1.

Extraction method Day 1 Weight (mg) Yield (%) Example 1
(Ultra high pressure extraction) 201.5 1.01 Control group
(Atmospheric pressure extraction) 37.27 0.19

As can be seen from the above Table 1, it was confirmed that the yield of Example 1 of the present invention was significantly better than that of the control group.

[ Experimental Example  2] Goat ginseng  Extractive Crude saponin  Content analysis

The crude saponin content of the goat's ginseng extract solution and the control group obtained in Example 1 was determined by dissolving each sample in methanol and passing through a 0.45 μm PVDF syringe filter (Whatman, Maidstone, England), followed by HPLC (1200 series, Agilent Technologies , Santa Clara, Calif., USA), and the amount of crude saponin was analyzed by quantitative analysis.

The column was eluted with a Kinetex C18 column (4.6 × 150 mm, Phenomenex, USA), the detector was UV (210 nm), the mobile phase was acetonitrile (A) and water (B) for HPLC grade for 0-5 minutes, 5% A; 5 to 40 minutes 95% A; 40-45 minutes and 95% A, the flow rate was 1.0 mL / min, and the sample injection amount was 10 μL. Then, the total peak area of the chromatogram at 210 nm was calculated and compared, and it is shown in Fig.

As can be seen from Fig. 1, the extraction rate of the sample subjected to ultra-high pressure treatment using water was better than that of the atmospheric pressure sample extract not subjected to ultra-high pressure treatment by about 10.2 times on day 0 and 2.9 times on day 1, Was increased by the ultra high pressure extraction process.

[ Experimental Example  3] Goat ginseng  Extraction and Analysis of Volatile Flavor Components from Extracted Liquids

For extracting the volatile flavor components of the ginseng extract and control group obtained in Example 1, 2 mL of each sample was placed in a 20 mL vial, and the mixture was maintained at 80 DEG C. Then, the SPME was vertically set and the volatile flavor The components were adsorbed onto PDMS / DVB fiber (polydimethylsiloxane / divinylbenzene 65 μm, Supelco, USA). Volatile fractions were collected by SPME fiber injected into GC injector, volatile components were desorbed for 3 minutes and then split by 5: 1. The volatile fragrance components of the extract solution of high-pressure treated goat ginseng and goat ginseng extract were analyzed by GC-MS. The GC-MS was connected to 5975C mass selective detector (Agilent Technologies Inc., Chandler, AZ, USA) Agilent 7890A Series GC system (Agilent Inc., USA) was used. Columns were HP-5MS 5% phenyl methyl Silox nonpolar (30 m × 250 μm × 0.25 μm). The GC column temperature was maintained at 100 ° C. for 2 minutes and then heated to 260 ° C. at a rate of 5 ° C./min and maintained at that temperature for 3 minutes. The injector temperature was set at 250 ° C., Was maintained at 1 mL / min. Ionization of the separated compounds was performed by electron impact ionization. The voltage and the temperature of the source were set at 70 eV and 250 ° C respectively, and the range of the molecular weight to be analyzed was set at 33 to 550 (m / z). GC-MS analysis of each peak separated by total ionization chromatogram (TIC) was carried out using the built-in WILEY library (registry of mass spectral data, 6th deion, USA) and NIST library (mass spectral search program , version 4.5, USA) were used together to identify the respective fragrance components, which are shown in Table 2 and FIG.

Retention time (minutes) compound Peak area Ultra high pressure untreated liquid extraction at room temperature Extra-high pressure liquid extraction liquid 5.65 Octanol 519,655 - 8.89 Benzenepropanol 461,430 - 9.56 Nonanoic acid 1,742,742 774,203 10.58 Cuminic aldehyde 2,659,912 - 11.33 Bicycloelemene 2,494,123 2,048,650 12.45 β-Panasinsene 4,316,416 4,939,766 12.57 β-Elemene 3,105,624 2,985,742 13.29 trans-Caryophyllene 1,117,111 1,181,731 13.58 Calarene 3,477,622 4,729,787 13.87 β-Farnesene 2,375,933 1,126,042 14.06 α-Humulene 13,141,476 15,983,555 14.23 Aromadendrene 1,258,422 1,143,061 14.49 β-Selinene 814,460 916,721 15.00 Bicyclogermacrene 9,154,322 7,568,346 15.52 δ-Cadinene 1,527,729 945,778 16.29 Farnesol 2,427,665 1,557,014 16.81 (+) - Spathulenol 4,287,157 6,443,199 17.02 Diethyl phthalate 23,097,682 74,365,817 18.07 Isopathulenol 2,784,428 4,408,305 18.40 α-Cadinol 2,750,402 1,768,660

As can be seen from Table 2 and FIG. 2, it can be seen that the aroma component of the ginseng is significantly increased by the treatment of the present invention.

 [ Experimental Example  4] Ultra high pressure After processing Goat ginseng  Possibility of long-term storage of raw material

To examine the storage ability of the ginseng herb extracted from the ultra-high pressure extrudate obtained in Example 2, it was stored at 2-8 degrees in a refrigerator for 84 days to periodically evaluate the sensory properties including visual observation. The shape after 84 days of each raw material is shown in Fig.

According to FIG. 3, it can be confirmed that the goat wild ginseng remains intact even after a long period of time since the treatment.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

(A) treating the silk ginseng vacuum-packed with water at a pressure of 200 to 400 MPa for 7 to 13 minutes;
(B) treating the treated ginseng with a pressure of 0.05 to 1.5 MPa for 3 minutes to 7 minutes to obtain a processed product of goat ginseng; And
(C) A method for simultaneously obtaining an extract of a ginseng-extracting ginseng having an effective component and a ginseng ginseng, which comprises separating the ginseng ginseng extract liquid phase and the ginseng ginseng from the ginseng ginseng product.
The method according to claim 1,
The method includes the steps of: (A) repeating the step (B) two to four times, and then proceeding to step (C), a method of simultaneously obtaining the active ingredient-rich goat ginseng extract liquid phase and the goat ginseng .
3. The method of claim 2,
The method further comprises the step of (B ') allowing the ginseng extract of the ginseng extract obtained after the step (B) repeatedly to be left at 2 to 8 캜.
The method of claim 3,
Wherein the step (B ') is carried out for 1 to 3 days, wherein the step (B') is carried out for 1 to 3 days.
The method according to claim 1,
The active ingredient may be selected from the group consisting of? -Panasinsene, Calarene,? -Humulene, (+) - Spathulenol, Diethyl phthalate, phthalate, and isopathulenol, wherein the active ingredient-promoted herb ginseng extract liquid phase and the ginseng ginseng are simultaneously obtained.
(1) treating the ginseng roots germinated in vacuum with water at a pressure of 200 to 400 MPa for 7 to 13 minutes;
(2) treating the goat ginseng at a pressure of 0.05 to 1.5 MPa for 3 minutes to 7 minutes to obtain a goat ginseng processed product;
(3) separating only the goat ginseng among the above-mentioned goat ginseng products; and
(4) Vacuum packaging the separated ginseng ginseng by a method of increasing the storage stability of ginseng ginseng,
The method includes repeating (1) and (2) two to four times.

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