KR101342090B1 - Physical property evaluation method of bio tissue and regenerated tissue using micro stage, and apparatus thereof - Google Patents

Abstract

The present invention relates to a method for evaluating the physical properties of living tissue and regenerated tissue using a micro-stage, and apparatus therefor, comprising: a driving unit 10 generating a load for generating a displacement at a constant speed and transferring the same to a test object; A fixing part 20 which fixes the test object and transmits the load generated in the driving part 10 to the test object; And a measuring unit 30 measuring the magnitude of the load generated by the displacement and the displacement actually occurring in the test object. Therefore, it is possible to investigate the properties of living tissue and regenerated tissue, which is impossible with the existing material tester, and can be used for the development of new biomaterials or medical research. And by applying mechanical stimulus directly to the test object and measuring the displacement of the material accordingly, the result can be obtained quickly and accurately. In addition, it is possible to evaluate the physical properties of synthetic resins such as polymers as well as biological tissues and regenerated tissues.

Micro stage, biological tissue, regenerative tissue, physical property evaluation, load cell, motor

Description

Physical property evaluation method of bio tissue and regenerated tissue using micro stage, and apparatus

1 is a perspective view showing the configuration of an apparatus for evaluating physical properties of living tissue and regenerated tissue using a micro stage according to a preferred embodiment of the present invention

Figure 2 is a perspective view showing a chuck (Chuk) of the fixture for fixing the test object in the present invention

Figure 3 is an exploded perspective view of the chuck according to a preferred embodiment of the present invention

4 is a configuration diagram of the jaw (jaw) used in the present invention

5 is a flowchart illustrating a method for evaluating physical properties of living tissue and regenerated tissue using a micro-stage according to a preferred embodiment of the present invention.

[Description of Code for Major Parts of Drawing]

10: drive unit 11: servo motor

12: reducer 20: fixed part

21: holder 22: feeder

30: measuring unit 31: load cell

32: LVDT (Linear Variable Differential Transformer)

40: test subject

100: micro stage 200a: first chuck

200b: second chuck 210: pin spring

220: jaw 221: irregularities

222 cuff

The present invention relates to a method and apparatus for evaluating physical properties of living tissues and regenerated tissues (hereinafter, also referred to as 'test subjects') using micro stages, and in particular, to investigate the physical properties of living tissues and regenerated tissues that are impossible with conventional material testers. The present invention relates to a method and apparatus for evaluating the properties of biological tissues and regenerated tissues using micro stages that can be used for the development of new biomaterials or medical research.

In the conventional case, a physical property is measured by measuring various action factors such as the temperature of a test material using a reactor. Because of this, there is a possibility that the result value may come out differently according to the phase change of the material, and there is a problem that it takes some time to obtain the result.

Accordingly, the present invention has been made to solve the above problems, and the first object of the present invention is to enable the investigation of the properties of living tissue and regenerated tissue, which is impossible with existing material testers, to develop new biomaterials or medical research. The present invention provides a method and apparatus for evaluating physical properties of living tissue and regenerated tissue using micro stages that can be used.

In addition, the second object of the present invention is to apply the mechanical stimulus directly to the test object (material) and measure the displacement of the material accordingly, thereby obtaining the properties of biological tissue and regenerated tissue using a micro stage that can accurately and quickly obtain a result. An evaluation method and its apparatus are provided.

In addition, a third object of the present invention is to provide a method and apparatus for evaluating the properties of biological tissues and regenerated tissues using micro stages, which are capable of evaluating not only biological tissues and regenerated tissues but also synthetic resins such as polymers.

Apparatus for evaluating physical properties of living tissue and regenerated tissue using a micro-stage according to the present invention for achieving the above object, the drive unit 10 for generating a load for generating a displacement at a constant speed to transfer to the test object; A fixing part 20 which fixes the test object and transmits the load generated in the driving part 10 to the test object; And a measuring unit 30 measuring the magnitude of the load generated by the displacement and the displacement actually occurring in the test object.

The drive unit 10, the servo motor 11 for generating a displacement at a constant speed; And a reducer 12 that adjusts the rotational speed of the servomotor by a reduction ratio to obtain a desired load and converts the rotational motion of the servomotor into a linear motion.

The fixing part 20, the fixing member having a first chuck (200a) for fixing one side of the test object; And a transfer table 22 having a second chuck 200b for fixing the other side of the test object and transmitting a load to the test object by a linear motion generated by the driving unit.

The first and second chucks 200a and 200b may include a plurality of jaws 220 fastened by pin springs 210; A first body portion 231 having a screw thread formed on an outer circumferential surface thereof, and a second body portion 232 integrally formed with a tong shape on one side of the first body portion 231 but smaller in diameter than the first body portion. And a grip body 230 in which a fixing groove 233 for fastening and fixing the pin springs to the first and second body parts is integrally formed. And a grip body shell 240 in which a screw thread 241 for fastening with a screw thread of the grip body is formed on an inner circumferential surface and an insertion hole 242 is formed on one side to insert the test object. When the grip body cell is rotated, the test object is fixed by changing the diameter while linearly moving along the thread.

The plurality of jaws have a concave-convex shape in order to reduce a slip phenomenon with the test object.

The plurality of jaws (jaw) is characterized in that provided with a cuff (cuff) to reduce the damage of the test object.

The measurement unit 30 is installed on the fixing rod, the load cell (Load cell) 31 for measuring the load applied to the test object and the displacement generated; And a linear variable differential transformer (LVDT) 32 installed between the fixed base and the transfer table and measuring a load applied to the test object and a displacement occurring therein.

The test subject is characterized in that one of the test subjects, including living tissue, regenerated tissue, synthetic resin.

In addition, the method for evaluating the physical properties of living tissue and regenerated tissue using a micro-stage according to the present invention for achieving the above object comprises the steps of: (a) fixing the test object to the fixing part; (b) generating a load for transferring a test object by generating a constant velocity displacement; (c) transferring a load to the test object; And (d) measuring the magnitude of the load generated by the displacement and the displacement actually occurring in the test object.

The test subject is characterized in that one of the test subjects, including living tissue, regenerated tissue, synthetic resin.

Therefore, the present invention can investigate the physical properties of living tissue and regenerated tissue, which is impossible with the existing material tester, and thus can be used for the development of new biomaterials or medical research.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Example

1 is a perspective view showing the configuration of an apparatus for evaluating physical properties of living tissue and regenerated tissue using a micro stage according to a preferred embodiment of the present invention. And, Figure 2 is a perspective view showing a chuck (Chuk) of the fixing fixture for fixing the test object in the present invention, Figure 3 is an exploded perspective view of the chuck according to a preferred embodiment of the present invention, Figure 4 is a jaw (jaw) used in the present invention ) Is a configuration diagram.

Apparatus for evaluating physical properties of living tissue and regenerated tissue using a micro-stage according to the present invention, as shown in Figure 1, the driving unit 10, the test object to fix the displacement at a substantially constant speed, the drive unit 10 It comprises a fixed part 20 for transmitting the generated load to the test object, the measuring unit 30 for measuring the magnitude of the load generated by the displacement and the displacement actually occurring in the test object.

First, the driving unit 10 is composed of a servo motor 11 and a reducer 12. In this case, the servomotor 11 serves to generate displacement at a constant speed, and the reducer 12 adjusts the reduction ratio to obtain a desired load by changing the rotational speed of the servomotor 11. The speed reducer 12 also simultaneously performs a function of converting the rotational motion of the servomotor 11 into a linear motion.

Next, the fixing unit 20 is an apparatus for fixing the test object and transmitting the load generated by the driving unit 10 to the test object, as shown in FIGS. 1 and 2, the work of the test object. A holding table 21 having a first chuck 200a for fixing a side, and a second chuck 200b for fixing the other side of the test object, and being loaded to the test object by a linear motion generated by the driving unit. Consists of including; transfer table 22 to deliver.

Here, the first and second chucks (200a, 200b), as shown in Figure 3, a plurality of jaws (220) fastened by the pin spring 210 and the thread formed on the outer peripheral surface The first body portion 231, the second body portion 232 is formed integrally in one side of the first body portion 231 in the shape of a forceps, the diameter is smaller than the first body portion, and the first and A grip body 230 having a fixing groove 233 integrally formed by fastening the pin spring to a second body part; And a grip body shell 240 in which a screw thread 241 for fastening with a screw thread of the grip body is formed on an inner circumferential surface and an insertion hole 242 is formed on one side to insert the test object. do. In this case, the plurality of jaws 220 have a concave-convex shape to reduce a slip phenomenon with the test object, and a cuff 222 to reduce breakage of the test object.

The first and second chucks 200a and 200b have a plurality of jaws 220 meshed with pin springs 210, and the grip body 230 includes the jaw 220. The jaw 220 is surrounded. In addition, the grip body 230 and the jaw 220 are connected by a screw thread. When the grip body cell 240 is rotated, the jaw 220 moves linearly along the screw thread while changing the diameter. 40).

The jaw 220 is processed to have irregularities to reduce slip between the jaw and the test object, and an expandable cuff in order to minimize breakage caused by the jaw. 222). The expandable cuff is a kind of sponge-like material, and has a property of absorbing and expanding a liquid such as water.

The assembling method of the first and second chucks 200a and 200b having the above configuration is as follows.

First, the pin spring 210 is fastened to a hole drilled in the lower portion of the jaw 220. Thereafter, the pin spring 210 coupled to the jaw 220 is fastened and fixed to the fixing groove 233 of the grip body 230, and then the screw thread and the grip body cell of the grip body 230 are fixed. The assembly is completed by threading the internal thread of 240).

Accordingly, by adjusting the height by rotating the grip body cell 240, the test object 40 is stably fixed using the two jaws 220 fixed to the pin spring 210.

In general, since living tissues and regenerated tissues have a problem of being easily broken even under a small load, in order to solve these problems, a grip for living tissues has been manufactured in the present invention.

Finally, the measuring unit 30 is composed of a load cell 31 and a linear variable differential transformer (LVDT) 32 for measuring a load applied to the test target and a displacement generated in the test process, respectively. The load cell 31 is installed on the holder 21, and the LVDT 32 is installed between the holder 21 and the feeder 22.

On the other hand, since the load and the displacement is a very important factor in the physical property evaluation, accurate measurement is necessary. In general, biological tissues and regenerated tissues use a product that can be precisely measured because breakage occurs under a small load compared to metal materials. In particular, because the object or scale of the test is not large, even a small error may produce a very wrong result.

The driving unit 10, the fixing unit 20, and the measuring unit 30 are installed and tested on a fixture manufactured to fix all the components in order to minimize the influence of the external environment.

Next, the physical property evaluation method of the physical property evaluation apparatus of living tissue and regenerated tissue using the micro stage which has the said structure is demonstrated with reference to FIG.

First, the test object 40 is fixed to the chuck of the fixing part 20 (step S10).

Then, the drive unit 10 generates a displacement at a constant speed to generate a load for transferring to the test object 40 (step S20).

Then, the load generated in the drive unit 10 is directly transmitted to the test object 40 through the fixing unit 20 (step S30).

Next, the magnitude of the load generated by the displacement in the measuring unit 30 and the displacement actually occurring in the test object 40 are measured (step S40).

Here, the test object 40 includes all synthetic resins, such as biological tissue, regenerated tissue, polymer (polymer).

The present invention can be seen as a constant material tester by measuring mechanical displacement by directly applying a mechanical stimulus to a material rather than indirectly measuring physical properties through electrical signals of various existing working factors. Therefore, it is possible to evaluate more precisely, and the test subjects mainly manufactured biological tissue or regenerated tissue, but it is also possible to evaluate the physical properties of synthetic resin such as polymer.

In addition, the present invention is an instrument that can investigate the properties of biological tissue and regenerated tissue that is impossible with the existing material tester, through which it can be used for the development of new biomaterials or medical research.

The present invention is not limited to the above-described embodiments, and various modifications and changes can be made by those skilled in the art, which should be regarded as included in the spirit and scope of the present invention as defined in the appended claims. will be.

As described above, according to the method and apparatus for evaluating the physical properties of living tissues and regenerated tissues using the micro-stage according to the present invention, as a mechanism for evaluating the material properties of various living tissues and regenerated tissues, It is possible to test materials of various living tissues and regenerated tissues, and can be widely used in medical tissue research and biomaterials research fields.

In addition, it is possible to investigate the physical properties of living tissue and regenerated tissue, which is impossible with the existing material tester, and can be used for the development of new biomaterials or medical research.

In addition, when the physical properties of biological tissues and regenerated tissues are more accurately measured using the equipment of the present invention, it is possible to create breakthrough added value when used in the bio-industry to study using the same or a measurement device or measurement industry connected thereto.

In addition, it is possible to replace expensive equipment capable of evaluating the properties of living tissue and regenerated tissue to date, thereby securing wide marketability through the present invention.

In addition, in the future, when specific physical properties are required for living tissues and regenerated tissues, specialized organizations can be specialized as evaluation apparatuses.

Claims (10)

In the physical property evaluation device of living tissue and regenerated tissue using a micro stage, A driving unit 10 generating a load for generating a displacement at a constant speed and transmitting the same to a test object; A fixing part 20 which fixes the test object and transmits the load generated in the driving part 10 to the test object; And It is configured to include; including; measuring unit 30 for measuring the magnitude of the load generated by the displacement and the displacement actually occurring in the test object, The fixing part 20 is: Fixture 21 having a first chuck (200a) for fixing one side of the test object; And It includes a second chuck (200b) for fixing the other side of the test object and the transfer table 22 for transmitting a load to the test object by a linear motion generated in the drive unit; The first and second chucks 200a and 200b are: A plurality of jaws 220 fastened by pin springs 210; A first body portion 231 having a screw thread formed on an outer circumferential surface thereof, and a second body portion 232 integrally formed with a tong shape on one side of the first body portion 231 but smaller in diameter than the first body portion. And a grip body 230 in which a fixing groove 233 for fastening and fixing the pin springs to the first and second body parts is integrally formed. And And a grip body shell 240 in which a screw thread 241 for fastening with the thread of the grip body is formed on an inner circumferential surface and an insertion hole 242 is formed on one side to insert the test object. , Rotating the grip body cell, the physical property of the biological tissue and regeneration tissue using a micro-stage, characterized in that for fixing the test object through a diameter change while linear movement along the thread. The method of claim 1, wherein the drive unit 10 is: A servo motor 11 generating displacement at a constant speed; And Reducing the rotational speed of the servo motor by the reduction ratio to obtain a desired load, and reducer 12 for converting the rotational motion of the servo motor to linear motion; biological tissue and regeneration using a micro-stage comprising a Device property evaluation device. delete delete The method of claim 1, wherein the plurality of jaws are: Apparatus for evaluating physical and regenerated tissue properties using a micro stage, characterized in that having a concave-convex shape in order to reduce the slip phenomenon with the test object. The method of claim 1 or 5, wherein the plurality of jaws are: Apparatus for evaluation of physical properties of living tissue and regenerated tissue using a micro stage, characterized in that the cuff (cuff) is provided to reduce the damage of the test object. The method of claim 1, wherein the measuring unit 30 is: A load cell 31 installed on the fixed base and measuring a load applied to the test object and a displacement occurring; And A biological tissue using a micro-stage, which is installed between the fixed base and the transfer table, and includes a linear variable differential transformer (LVDT) 32 measuring a load applied to the test object and a displacement occurring therein. Apparatus for evaluating the properties of regenerated tissue. The method of claim 1, wherein the test subject is: Apparatus for evaluation of physical properties of biological tissue and regenerated tissue using a micro-stage, characterized in that one of the test targets, including biological tissue, regenerated tissue, synthetic resin. delete delete

Images