KR101309844B1 - Herbal medicine for enhancing anticancer activity and manufacturing method thereof - Google Patents

Abstract

PURPOSE: An oriental medicinal material for enhancing anticancer activity is provided to show excellent anticancer effect. CONSTITUTION: A method for manufacturing an oriental medicinal material with anticancer activity comprises the steps of: reacting non-arsenic minerals and Erigeron Canadensis L. mixed in a weight ratio of 0.7:1-1:0.7; inputting the mixture into a pottery and sealing; and heating the pottery. The non-arsenic mineral includes orpiment and/or arsenic trioxide.

Description

Herbal medicine for enhancing anticancer activity and preparation method thereof {HERBAL MEDICINE FOR ENHANCING ANTICANCER ACTIVITY AND MANUFACTURING METHOD THEREOF}

The present invention relates to a herbal medicine for enhancing anticancer activity and a preparation method thereof. More specifically, the present invention relates to a herbal medicine for enhancing anticancer activity and a method for preparing the same, which are formulated with arsenic minerals such as Xinseok Emergency and Sukwoong Hwang by oriental medicine legislation.

Cancer is an uncontrolled, endless division of cells that can spread through blood or lymphatic vessels to other organs. That means malignant neoplasms. Neoplasms are malignant and benign, and when they are malignant, they grow very fast and can spread cancer cells to other distant organs through blood or lymphatic vessels, thereby spreading cancer to other organs. Despite the striking advances in the regulation of cell cycle and apoptosis and the search for new targets including oncogenes and cancer suppressor genes to conquer cancer, the incidence of cancer is increasing as civilization develops.

Cancer treatments generally include surgery, radiotherapy, chemotherapy, immunotherapy and gene therapy, and dual chemotherapy is the most widely used. However, the anticancer drugs used in chemotherapy have cytotoxicity not only to cancer cells but also to normal cells, causing various side effects. Specifically, it is reported that gastrointestinal disorders, bone marrow hematopoietic disorders, etc. Research is actively underway to alleviate chemotherapy side effects through pharmacokinetic medicine.

Arsenic has long been thought to be a poison, and in the 1820s it was known as a potent environmental carcinogen that causes cancer in the skin and lungs. Biochemically, arsenic has been reported to bind to the sulfhydryl group, which is an activation site of the enzyme, to inactivate certain enzymes, to interfere with phosphorylation and dephosphorylation, and to cause chromosomal abnormalities. However, it has been documented in the literature that low concentrations of arsenic compounds have been used in the treatment of diseases for a long time, both east and west. Attempts to develop arsenic compounds as cancer treatments based on these pharmacological actions have been conducted relatively recently, and are currently being actively studied. Especially, oriental traditional medicine meets the mainstream of modern cancer chemotherapy. Molecular medicine is trying to be integrated. In the case of arsenic trioxide (As ₂ O ₃ ), which is a type of arsenic compound, (1) blocking blood vessels to cancer tissues; (2) inhibition of telomerase transcription (Cancer Research, 59, 6033-6037, December 15, 1999); And (3) the mechanism of action of cancer cell apoptosis (The J. of Clinical Investigation, Nov. 2001, 108: 10, 1541-1547), but the side effects of arsenic trioxide have been a problem. .

On the other hand, the new stone emergency (신 石 砒霜), which is used as a herbal medicine, is also called Shinseok or Shinju, and it is a crystal obtained by processing non-ore, which is high purity arsenic trioxide (As ₂ O _3). Has been used to obtain However, nephritis has a disadvantage that it can interfere with complete absorption in the body as a mineral. Caustic soda (NaOH) was used as a method to solve this drawback, but there was a problem that can cause harmful toxicity to the human body when over-commercial use.

Seok Ung Hwang (石 雄黃), which is used as a medicinal herb, is also called Ung Hwang (雄黃) and is a kind of mineral with a red color, to obtain high purity arsenic disulfide (As ₂ S ₂ ) or arsenic trisulfide (As ₂ S ₃ ). Has been used.

Stone salt is a mineral produced in nature and contains sodium nitrate (NaNO ₃ ), and it has been used for the treatment of various diseases such as asthma.

Manganese, which is used as a medicinal herb, contains sodium sulfate (Na ₂ SO ₄ · 10H ₂ O) or sodium sulfate (Na ₂ SO ₄ ), and also contains a small amount of inorganic salts such as sodium chloride, magnesium chloride, and magnesium lactate. After taking it, it is not easily absorbed and forms a high semen to increase the water in the intestine and stimulate the intestines to enhance peristalsis to help bowel movements.

In order to solve the above problems, the present inventors have repeatedly studied the arsenic minerals by applying the herbal medicine method, and prepared a herbal medicine for enhancing anticancer activity, which is harmless to the human body and is excellent in anticancer activity. It was.

An object of the present invention is to provide an anti-cancer activity enhancing herbal medicine excellent anti-cancer activity effect.

Another object of the present invention is to provide a method of manufacturing a herbal medicine for enhancing anticancer activity.

One aspect of the present invention is to provide an herbal medicine for enhancing anticancer activity.

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Another aspect of the present invention is to provide a method of manufacturing the herbal medicine for enhancing the anticancer activity. In one embodiment, the method for preparing an herbal medicine for enhancing anticancer activity includes reacting arsenic minerals and forget-me-not, and the arsenic minerals include at least one of nephritis and urine.

In another embodiment, the method for preparing herbal medicine for enhancing anticancer activity includes the step of reacting arsenic minerals and saltpeter, and the arsenic minerals include at least one of nephritis and urine.

In one embodiment, the mixture containing the arsenic mineral and forget-me-not to the earthenware and sealing; And heating the earthenware.

In another embodiment, the mixture comprising the arsenic mineral and saltpeter in the earthenware and sealing; And heating the earthenware.

In one embodiment, the step of grinding the product inside the heated earthenware; further includes.

The arsenic mineral and forget-me-not is characterized in that it is included in a weight ratio of 0.7: 1 ~ 1: 0.7.

The arsenic minerals and saltpeter are characterized in that included in a weight ratio of 0.3: 1 to 1: 0.3.

And cooling the heated earthenware and releasing a gas inside the earthenware.

In one embodiment, further comprising the step of pulverizing the product inside the earthenware to a size of 10 ~ 500㎛ after releasing the gas inside the earthenware.

The herbal medicine for enhancing anticancer activity according to the present invention may have excellent anticancer activity.

In the following description of the present invention, when it is determined that detailed descriptions of related well-known technologies or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to be exemplary, self-explanatory, allowing for equivalent explanations of the present invention.

In addition, “sodium sulfate” (function) described herein is defined to include both sodium sulfate (Na ₂ SO ₄ ) and sodium sulfate (Na ₂ SO ₄ · 10H ₂ O).

One aspect of the present invention relates to a method for manufacturing the herbal medicine for enhancing the anticancer activity. The manufacturing method may include (a) a mixture input step and (b) a heating step.

In one embodiment, the method for preparing the herbal medicine for enhancing anticancer activity includes the step of reacting arsenic minerals and forget-me-not, and the arsenic minerals include at least one of nephritis and urine.

In another embodiment, the method for preparing an herbal medicine for enhancing anticancer activity includes the step of reacting arsenic minerals and saltpeter, and the arsenic minerals include at least one of nephritis and urine.

Hereinafter, the configuration of the present invention in more detail step by step.

(a) Mixing step

The step is to put a mixture containing arsenic minerals and forget-me-not to the earthenware and to seal.

The arsenic minerals and forget-me-not can be used that are commonly used in the field of herbal medicine.

As the arsenic mineral may be used such as Xinseok emergency and Seokwoong sulfur. These can be used 1 type or in mixture of 2 or more types.

In one embodiment, the arsenic mineral and forget-me-not may be included in a weight ratio of 0.7: 1 to 1: 0.7. Preferably it may be included in a weight ratio of 0.8: 1 to 1: 0.8. More preferably, it may be included in a weight ratio of 1: 1. In the above range, the arsenic mineral is legally made and is harmless to the human body, thereby optimizing an anticancer activity effect.

In addition, the mixture containing the arsenic mineral and saltpeter in the step may be put into the earthenware and sealed.

The cornerstone may be used that is commonly used in the field of herbal medicine.

At this time, the arsenic minerals may be used such as the neolithic emergency and Seokwoong sulfur. These can be used 1 type or in mixture of 2 or more types.

In one embodiment, the arsenic mineral and saltpeter may be included in a weight ratio of 0.3: 1 to 1: 0.3. Preferably it may be included in a weight ratio of 0.4: 1 to 1: 0.4. More preferably, it may be included in a weight ratio of 0.5: 1. In the above range, the arsenic mineral is legally made and is harmless to the human body, thereby optimizing an anticancer activity effect.

In one embodiment, the inlet of the earthenware can be sealed with mud. When the sealing does not enter oxygen into the earthenware can easily maximize the legal effect of the arsenic mineral in the present invention.

Moreover, it is preferable to use earthenware excellent in heat resistance as said earthenware.

(b) heating step

The step is heating the sealed earthenware.

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The earthenware may be heated for 10 to 24 hours at 400 ~ 800 ℃. Under these conditions, a complete legalization of the arsenic mineral can be achieved.

In one embodiment, after the heating step may further comprise the step of grinding the product inside the heated earthenware. In one embodiment, the product inside the heated earthenware may be ground to 10 ~ 500㎛. When pulverized to the size of the above range, the herbal medicine for enhancing the anticancer activity of the present invention can be easily included in the pharmaceutical preparations can be prepared, and the anticancer activity can be excellent.

In another embodiment, after the heating step may further comprise the step of releasing the gas inside the earthenware after cooling the heated earthenware. Under the above conditions, the toxic components in the gaseous state inside the earthenware can be easily removed, thereby further improving the anticancer activity effect of the present invention.

At this time, the step of pulverizing the product inside the earthenware after releasing the gas inside the earthenware. In one embodiment, the product inside the heated earthenware may be ground to a size of 10 ~ 500㎛. When pulverized to the size of the above range, the herbal medicine for enhancing the anticancer activity of the present invention can be easily included in the pharmaceutical preparations can be prepared, and the anticancer activity can be excellent.

Another aspect of the present invention relates to a herbal medicine for enhancing anticancer activity. In one embodiment, the herbal medicine for enhancing anticancer activity includes a product prepared by reacting hydrous sodium sulfate and arsenic minerals derived from forget-me-not as an active ingredient.

In another embodiment, the herbal medicine for enhancing anticancer activity includes a product prepared by reacting hydrated sodium sulfate and arsenic minerals derived from saltpeter as an active ingredient.

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The arsenic mineral may be included in one or more of the new stone emergency and Seokwoong sulfur.

In the case of using the anti-cancer activity-enhancing herbal medicines, various known methods can be applied in the preparation of pharmaceutical preparations and their administration methods. For example, the herbal medicine for enhancing anticancer activity may be administered through various routes including parenteral, oral (dermal, subcutaneous, intravenous, intramuscular, abdominal, etc.).

There is no particular limitation on the formulation as long as it is prepared including the anti-cancer activity enhancing herbal ingredients, and may have, for example, formulations such as pills, powders, tablets, capsules, solutions, granules, injections, and the like. When formulating an herbal medicine for enhancing anticancer activity as described above, various excipients used in medicine, such as carriers or other additives such as stabilizers, emollients, emulsifiers, etc., may be used, if necessary, as long as they do not adversely affect the active ingredient.

In addition, the dosage and administration method of the herbal medicine for enhancing anticancer activity may be varied according to the age, weight, physical condition and amount of cancer cells of the patient. For example, it may be used within the range of 5-50 mg / kg (weight) / day before or during radiation.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to preferred embodiments of the present invention. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited to the following examples. Details that are not described herein will be omitted since those skilled in the art can sufficiently infer technically.

Manufacturing example  One

Neolithic emergency and forget-me-not mixed and put into earthenware and sealed the inlet with clay. The earthenware was heated at 500 ° C. for 20 hours, then cooled to room temperature and the gas inside the earthenware was discharged to obtain a mixture. The obtained mixture was ground to a size of 100㎛ to prepare a herbal medicine for enhancing anticancer activity.

Manufacturing example  2

Seokwoong sulfur and forget-me-not mixed into the earthenware and prepared the herbal medicine for anticancer activity enhancement in the same manner as in Preparation Example 1.

Manufacturing example  3

A mixture of nephrolithiasis and saltpeter was added to earthenware and a herbal medicine for enhancing anticancer activity was prepared in the same manner as in Preparation Example 1.

Manufacturing example  4

Seok-woong sulfur and salt stone were mixed and put into earthenware, and a herbal medicine for enhancing anticancer activity was prepared in the same manner as in Preparation Example 1.

Comparative example  One

After the reaction with caustic soda in the nephritis ratio was prepared a herbal medicine for enhancing anticancer activity.

Test Example  1: residual NaOH Volume measurement

Residual NaOH amount was measured for the anticancer activity enhancing herbal preparations prepared in Preparation Examples 1 to 4 and Comparative Example 1, respectively. As a result, NaOH was not detected in the herbal medicine for enhancing anticancer activity according to Preparation Examples 1 to 4, but NaOH was detected in the herbal medicine prepared in Comparative Example 1. Therefore, it was confirmed that the herbal medicine for enhancing anticancer activity of the present invention does not contain NaOH with respect to arsenic trioxide formulated using caustic soda.

Example  One

5 mg of the herbal medicine for enhancing anticancer activity prepared in Preparation Example 1 was mixed with 150 mg of legal ginseng and prepared in a vacuum capsule. In this case, the legal ginseng was put into a sealed container with 6 years old ginseng, aged at room temperature for 4 weeks, and extracted by lyophilization.

Example  2

10mg of anti-cancer activity enhancing herbal medicine prepared in Preparation Example 2 was mixed with 150mg of legal ginseng and prepared in a vacuum capsule. In this case, the legal ginseng was put into a sealed container with 6 years old ginseng, aged at room temperature for 4 weeks, and extracted by lyophilization.

Example  3

10 mg of the anti-cancer activity enhancing preparation prepared in Preparation Example 3 was added to 20 ml of the legal ginseng distilled extract obtained by distilling the legal ginseng used in Examples 1 to 2 to prepare a herbal acupuncture solution.

Test Example  2 to 6

Test Example  2

Name: Kim *** (Age 54)

Diagnosis date: 2013.02.15. Hana Integrated Medical Center

Diagnosis: Suspected recurrence of rectal cancer (CEA level 42.41 ng / ml)

History: During the course of diagnosis of rectal cancer and postoperative (stage IIIA) adjuvant chemotherapy, the patient was admitted to oriental medical therapies with suspected recurrence (PET-CT recurrence) due to elevated tumor marker (Carcinoembryonic antigen, CEA). .

Dosing period: 2013.02.15 ~ 2013.03.06

During the dosing period, each of Example 1 and Example 2 was taken once a day and the acupuncture of Example 3 was performed once a day, indicating that the tumor marker value (CEA) was reduced from 42.41 ng / ml to 34.15 ng / ml. It was.

Test Example  3

Name: Jeong *** (At that time 67)

Diagnosis date: 2013.02.18. Hana Integrated Medical Center

Diagnosis: Prostate Cancer

Medical history: Prostate specific antigen (PSA) levels were high (5.3, 5.4, 5.5 ng / ml). Ultrasonography was performed in the hospital and histological examination was recommended. 15 February biopsy confirmed prostate cancer).

Duration of medication: 2013.01.19. ~ 2013.02.23

Example 1 and Example 2 were taken once daily during the dosing period, and the change in PSA test values according to the passage of time while performing the medicinal acupuncture of Example 3 is shown in Table 1 below.

Inspection date PSA test value (ng / ml) 2011.08.09 5.23 2011.12.01 3.58 2012.06.19 5.12 2012.12.11 5.47 2013.01.19 4.00 2013.02.04 6.98 2013.02.18 2.71 2013.02.23 2.37

When applying the herbal medicine for enhancing the anticancer activity of the present invention it was confirmed that the PSA level is lowered as shown in Table 1.

Test Example 4

Name: Hur ** (age 76)

Diagnosis date: 2013.02.20. Hana Integrated Medical Center

Diagnosis: Lung cancer

History: After diagnosis of nodule in the lung area (T2, NO, M0), tumor marker level (CEA) continued to rise, and we visited our hospital.

Duration: 2013.02.20 ~ 2013.03.02

During the dosing period, each of Example 1 and Example 2 was taken once a day and the acupuncture of Example 3 was performed once a day, indicating that the tumor marker value (CEA) was reduced from 29.89 ng / ml to 20.60 ng / ml. It was.

Test Example  5

Name: Park ** (at the time 40)

Diagnosis date: 2013.01.29. Hana Integrated Medical Center

Diagnosis: Thyroid medulla

History: The patient was diagnosed with medullary thyroid carcinoma and operated. The thyroid medullary carcinoma specific antigen (calcitonin, Calcitonin) level was high (8.0 pg / ml). Visited the treatment car (January 29, 2013).

Duration of medication: 2013.01.29 ~ 2013.03.11

As a result of taking twice daily Example 1 during the administration period, it was confirmed that the tumor marker value (Calcitonin) was reduced from 8.0 pg / ml to 3.1 pg / ml.

Test Example 6

Name: Kim ** (Age 34)

Date of Diagnosis: 2013.02.29 Hana Integrated Medical Center

Diagnosis: Uterine teratoma

History: He was diagnosed with uterine teratoma and recommended a surgical procedure at the hospital. However, he withheld the surgery, and during the follow-up, he visited the herbal treatment hospital with CA 19-9 levels.

Duration of medication: 2013.02.29 ~ 2013.03.13

As a result of taking twice daily Example 1 during the administration period, CA 19-9 levels were reduced from 84.92 U / mL to 74.07 U / mL in the intermediate test.

Claims (11)

delete delete Reacting the arsenic mineral and forget-me-not;
The arsenic mineral is an herbal medicine manufacturing method for enhancing anticancer activity, including one or more of Sinseok emergency and Seokwoong sulfur.
Reacting the arsenic mineral and the cornerstone;
The arsenic mineral is an herbal medicine manufacturing method for enhancing anticancer activity, including one or more of Sinseok emergency and Seokwoong sulfur.
The method of claim 3, wherein the method
Injecting and sealing a mixture comprising arsenic minerals and forget-me-not; And
Heating the earthenware;
Chinese herbal medicine manufacturing method for enhancing anticancer activity comprising a.
5. The method of claim 4,
Injecting and sealing a mixture comprising arsenic minerals and saltpeter into earthenware; And
Heating the earthenware;
Chinese herbal medicine manufacturing method for enhancing anticancer activity comprising a.
The method of claim 5 or 6, further comprising: grinding a product inside the heated earthenware;
Chinese herbal medicine manufacturing method for enhancing anticancer activity further comprising.
The method of claim 3, wherein the arsenic mineral and forget-me-not are prepared in a weight ratio of 0.7: 1 to 1: 0.7.
The method of claim 4, wherein the arsenic mineral and saltpeter are manufactured in a herbal composition for enhancing anticancer activity, characterized in that it is included in a weight ratio of 0.3: 1 to 1: 0.3.
The method of claim 5 or 6, further comprising releasing the gas inside the earthenware after cooling the heated earthenware.
The method of claim 5 or 6, further comprising pulverizing the product in the earthenware to a size of 10 to 500㎛ after releasing the gas inside the earthenware.