KR101290587B1 - Method for controlling injurious insect pests using ultrasound - Google Patents

Abstract

PURPOSE: A pest control method using ultrasound waves is provided to eradicate moths which are harmful to plants by using the ultrasound waves of specific frequency. CONSTITUTION: A pest control method using ultrasound waves comprises: a step of spraying chemicals on a plantation; and a step of improving the efficiency of chemical control on beet armyworms with the ultrasound waves of specific frequency which are generated by a sonicator. The frequency of the ultrasound waves is 30 kHz or 45 kHz. The intensity of the ultrasound waves is 70-95 dB. The multiple sonicators are installed in an equipment house. The ultrasonic generators facing each other are installed at regular intervals of 20-60 m. The ultrasound waves suppress the feeding activity of pests, change the nutrient content of blood plasma, increase blood sugar, control growth, prolong percent pupation and reduce an adult eclosion ratio.

Description

Method for controlling injurious insect pests using ultrasound}

The present invention relates to a pest control method, and more particularly to a pest control method using ultrasonic waves.

Some insects use sound as a means of communication to find mates. These sounds can be classified into a collision sound using a relatively low frequency and a friction sound and a vibration sound having a relatively high frequency. Collision is when a low-frequency sound pattern is emitted from a conch (Meconema thalassinum) sitting on a blade of grass and tapping the leaf surface at a constant speed with its hind legs, which is used as a communication signal to the surrounding species. On the other hand, when the mutual parts of the body have the structure of strings and friction pieces, the sounds generated by rubbing these two parts become friction sounds, and many kinds of insects such as grasshoppers and crickets communicate with these sounds. Vibration sounds can be found in cicadas that communicate with sounds generated by vibrating a thin vibrating membrane like a bow. The hearing of the insect that senses sound leads to a sensory sensor that converts a physical sonic signal into an electrical signal, a neurotransmission process that transfers it to the brain, and a corresponding behavioral response. Auditory accommodation varies with insects, such as the eardrum, the mechanical sensory hair, the lower lip beard of the hornhead, and the Johnston organ of the mosquito. Therefore, communication occurs as pairing of sound generation and hearing occurs, and communication using sound has species-specific frequency and frequency.

Sound communication is also seen in the relationship between insects and predators. Many insects have developed mechanisms to detect the sonic detection of insectivorous bats. The avoidance behavior for ultrasound is programmed in these insects as reflex motion, and the ultrasound received by the tympanic organ is detected by bat recognition and then connected to the motor nerve. In the case of night moths, they found an auditory nerve that detects the bat's sound detection signal and revealed the electrical response of the nerve.

The study of stress sound waves focused on vertebrates. In rats, stress-induced acute stress sound waves are triggered (antinociception), while for long-term stress sound exposure, they can cause chronic pain (fibromyalgin) or rheumatoid arthritis, causing stress sound waves to have a detrimental effect on the nervous and immune systems. Said. In addition, the Republic of Korea Patent Application No. 10-2002-0007488, a combination of the audio signal of the audio frequency band and the ultrasonic signal of the ultrasonic frequency band for pest control is stored, and during the reproduction of the pest control with the audible audio signal By simultaneously regenerating the ultrasonic signal, a technique is disclosed in which the pest is eliminated by the ultrasonic signal emitted from the audio device.

However, according to the prior art, a technique for controlling pests that harm plants using ultrasonic waves of a certain frequency while using medicinal plants while cultivating plants has not been proposed.

The above-described background technology is technical information that the inventor holds for the derivation of the present invention or acquired in the process of deriving the present invention, and can not necessarily be a known technology disclosed to the general public prior to the filing of the present invention.

The present invention provides a pest control method using ultrasonic waves for combating moths that harm plants using ultrasonic waves of a specific frequency during plant cultivation.

The technical problems other than the present invention can be easily understood from the following description.

According to one aspect of the invention, the step of treating the medicament to the plantation of the plant; And an ultrasonic generator for generating ultrasonic waves of a specific frequency to generate the ultrasonic waves on the plantation of the plant, thereby providing a pest control method using the ultrasonic waves to increase the efficiency of chemical control.

Here, the frequency of the ultrasonic waves may be 30 kHz or 45 kHz, the intensity of the ultrasonic waves may be 70 to 95 dB.

In addition, the ultrasonic wave generating apparatus may be installed in a facility house, the pest may be any one or more of the night moth, Chinese cabbage moth, American leaf oyster flies, peach or aphid.

In addition, the ultrasonic generating device is a plurality, the ultrasonic generating device looking at each other may be installed spaced 20m to 60m apart.

In addition, the ultrasound is characterized by inhibiting the feeding activity of the pest, varying the nutrient content of the plasma, increasing blood sugar, inhibiting the development of the dragon, extending the container, and lowering the rate of allegory to adult The ultrasound may lower the mating rate of the pest by 1/2 to 1/15 times.

In addition, the ultrasonic wave generating apparatus may periodically change the ultrasonic waves of different frequencies to generate in the plantation of the plant.

Other aspects, features, and advantages will become apparent from the following drawings, claims, and detailed description of the invention.

Insect control method using the ultrasonic wave according to the present invention can be used to combat the moths harming the plant by using the ultrasonic wave of a specific frequency during plant cultivation.

1 is a graph of larva feeding behavior analysis results of pest control treatment using ultrasonic waves according to an embodiment of the present invention.
Figure 2a to 2c is a biological material analysis of the results of pest control treatment using ultrasonic waves in accordance with an embodiment of the present invention.
Figure 3a to 3d is a view showing the gene expression of the results of pest control treatment using ultrasonic waves in accordance with an embodiment of the present invention.
Figure 4 is a graph showing the change in dragon growth of the pest control treatment results using ultrasonic waves according to an embodiment of the present invention.
5a to 5c is a view showing the adult mating activity of the pest control treatment results using ultrasonic waves according to an embodiment of the present invention.
Figure 6 is a block diagram of an ultrasonic wave generating apparatus for performing a pest control method using the ultrasonic wave in accordance with an embodiment of the present invention.
7A and 7B are diagrams illustrating the installation of an ultrasonic wave generator in a facility house according to an embodiment of the present invention.
7C and 7D illustrate insecticidal power of pest control treatment using ultrasonic waves according to an embodiment of the present invention.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Terms including ordinals, such as first, second, etc., may be used to describe various elements, but the elements are not limited to these terms. The terms are used only for the purpose of distinguishing one component from another.

When a component is referred to as being "connected" or "connected" to another component, it may be directly connected to or connected to that other component, but it may be understood that other components may be present in between. Should be.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The term " part, "" module," " means, "or the like, which is described in the specification, refers to a unit for processing at least one function or operation and may be implemented by hardware or software or a combination of hardware and software .

In the following description of the present invention with reference to the accompanying drawings, the same components are denoted by the same reference numerals regardless of the reference numerals, and redundant explanations thereof will be omitted. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

The effects of stress sound on insect physiological phenomena were analyzed in Spodoptera exigua, American leaf dens, and Peach aphids. All these insects exhibited disturbing physiological responses to the sound of 5,000 Hz. In the case of parchment moth, the larva's feeding activity was slowed, thus delaying the development and lowering the immune activity. These insects have also been shown to have an application effect that increases the effectiveness of insecticide control by reducing the resistance to insecticides when exposed to stress sound waves.

Stress sonication causes physiological changes in Spodoptera exigua. The present invention was created on the basis of the results of analyzing the effects of ultrasound (20 kHz or more) treatment on the feeding of the dragonfly moth larvae, dragon development and adult mating behavior. Sonication inhibited the feeding activity of five worms. Larvae, especially those treated with 30 kHz or 45 kHz, reduced their feeding activity by more than 50%. Larvae subjected to this sonication had a change in the plasma nutrient content. Plasma protein decreased with increasing frequency of treatment ultrasound. However, blood glucose increased with increasing frequency of treatment ultrasound. Lipid content increased until 30 kHz treatment and then decreased. Three tissues of blood cells, fat bodies and epidermal cells of Pabam moths expressed three types of stress-related genes: heat shock protein and apolipophorin III. However, ultrasound treatment inhibited the expression of these stress genes. Sonication also inhibited dragon development, extending the vessels and significantly lowering the allegory to adulthood. In addition, the ultrasonic treatment significantly suppressed mating behavior of the adult, significantly lowering the laying force of the female. On the basis of these results, the treatment of pabam moth and Chinese cabbage moth (Plutella xylostella) generated in outdoor packaging can significantly increase the biopesticide control effect.

Ultrasound is within the audible range of sound receivers but can act as stress sound waves that can disrupt normal physiology rather than true communication.

Hereinafter, materials, methods, and treatment results of the pest control method using the above-described ultrasonic wave target moth will be described in order.

The larvae were collected from green onion plantations in Andong-si for the purpose of rearing the chestnut moths. The conditions of the breeding incubator were 25 W 1 ° C and photoperiod 16: 8 h (L: D). As a food for adults, 10% sugar water was supplied to the laying box.

The sonic device was composed of a decibel measuring instrument, a sonic processing instrument (45 ㅧ 90 cm), and a sound wave transmitter for ultrasonic treatment. The analyzed beetroot moths were all five insects and the physiological changes were observed by fixing the frequency at 95 dB at room temperature of 25 변화 C and exposing them for 20 hours at 20-45 kHz ultrasound.

To analyze changes in heat shock proteins (hsp70, 74, 83) and apolipophorin (ApoLpIII) genes of Pabam moths under stress sonication, 10 Bambap moth larvae were examined for 24 hours in each ultrasonic (20-45 kHz) treatment group. After exposure, the tissues were dissected and each tissue (blood cell, fat body, epidermis) was extracted and RNA was extracted using Trizol solution (Invitrogen, Carlsbad, CA, USA). The extracted RNA was quantitatively analyzed and diluted to about 90 ng / μl using deionized distilled water to synthesize cDNA using reverse transcriptase (Bioneer, Daejon, Korea) and used for PCR. Among the heat shock proteins (hsp), the hsp70, hsp74, and hsp83 genes were amplified using specific primers. In this case, the primer sequence of SexHsp70 is 5'-CAT GAA TCC TCG CGC ACT GC-3 ', 5'-CCT TGT CGT TCT TGA TCA CG-3', and SexHsp74 is 5'-CCT ACC TGA ACA CCT CAG T-3 ', 5′-GGG ATC GTA GTA TTT CTG GTG-3 ′, SeHsp83 is 5′-GCT GAC ATT AGC ATG ATT GG-3 ′, 5′-GGC AGG TCC TCA CTG TCT AC-3 ′.

In addition, specific primers of the ApoLpIII gene were used. The primer sequence is 5'-ATG GTC GCC AAG TTG TTC GTG-3 ', 5'-CTG CTT GTT GGC AGC CTC-3'. The configuration of each PCR sample was as follows. 1 μl cDNA, 2.5 μl dNTP, 2.5 μl 10 × PCR buffer, 1 μl each primer (25 pmol / μl), 0.5 μl Taq polymerage, 16.5 μl tertiary distilled water. PCR reaction conditions were initially subjected to an inactivation step at 94 ° C. for 2 minutes, followed by an amplification step with 35 repetitions. The amplification process consisted of denaturation step at 94 ° C for 1 minute, and primer binding reaction consisted of chain extension step of 1 minute at 47, 45, 48, 53 ° C and 1 minute at 72 ° C for hsp70, hsp74, hsp83, and ApoLpIII, respectively. . The final chain extension step then continued at 72 ° C. for 10 minutes. PCR products were identified as 1% agarose gel in 1 × TAE (40 mM Tris-acetate, 1 mM EDTA, pH 8.0).

For analysis of plasma protein content, 10 larvae of Pabam moths were exposed to ultrasound (20-45 kHz) for 24 hours and then blood lymphocytes were extracted. The extracted blood lymphocytes were centrifuged at 250 x g for 5 minutes to obtain supernatant plasma. The measurement sample was added with 1 ml of 1x Bradford solution and 20 μl of plasma and then reacted at room temperature for 5 minutes to measure absorbance at a wavelength of 595 nm. The standard solution of quantitative analysis was obtained by using bovine serum albumin (Sigma-Aldrich Korea, Seoul, Korea).

Plasma samples were obtained after sonication as described above for plasma lipid content analysis. The measurement sample was reacted with 100 μl of plasma and 100 μl of lipid extraction solution (chloroform: methanol (1: 1), v / v) for 5 minutes and centrifuged at 12,500 x g for 5 minutes. The centrifuged supernatant was reacted for 20 minutes in a 95 ° C constant temperature water bath, 200 μl of sulfuric acid was added and reacted again for 10 minutes in a 95 ° C constant temperature water bath. After transferring to room temperature, 5 ml of Vanillin solution (600 mg Vanillin, 400 ml phosphoric acid) was added and the absorbance was measured at 525 nm. The standard curve was obtained from the lipid content standard equation using linseed oil (0-500 μg).

Plasma samples were obtained after sonication as described above for plasma free sugars analysis. The sample was reacted for 5 minutes after adding 300 μl of 2% Na 2 SO 4 and 600 μl of methanol to 100 μl of plasma. After centrifugation for 5 minutes at 12,500 x g. Supernatants were used for free sugar analysis. The supernatant was evaporated in a constant temperature water bath at 95 ° C. for 50 minutes to 200-300 μl. Thereafter, after transferring to room temperature, 5 ml of Anthrone solution (750 mg of Anthrone, 380 ml H 2 SO 4, 150 ml distilled water) was added, and the absorbance was measured at 625 nm. Standard curves were glucose (0-500 μg).

To test the larvae intake by sonication, the frequency was fixed at 95 dB for 5 months old insects (Day 2) and exposed for 24 hours under sonic conditions at 0, 20, 25, 30, 35, 40 and 45 kHz. The difference in feeding amount was investigated. Chinese cabbage leaves were used for feeding and calculated by the weight difference before and after treatment. To calculate the naturally reduced cabbage leaf weight, the reduced cabbage weight difference was calculated between the time before and after treatment under the same conditions. Each treatment was repeated three times with 10 animals.

For the dragon development assay by sonication, 10 pupas on the 1st day of solubilization were exposed in 3 repetitions at 25 ° C for each frequency of 0, 20, 25, 30, 35, 40 and 45 kHz, and the period until allegorization Measured. The rate of allegory was also analyzed by repetition.

One adult female and two males were placed in a 0.2 L cylinder (10 cm diameter x 10 cm height) with 10% sugar water for adult mating behavioral tests following sonication. The top of the vessel was covered with a mesh and exposed to an ultrasonic speaker for sound wave treatment. Each sonication consisted of different frequencies of 0, 20, 25, 30, 35, 40 and 45 kHz and treated for 4 days. Since the number of copulation per female was determined by the number of sperm bags in the female copulation bag. In addition, the number of eggs scattered in the container was counted to evaluate the laying power. Each treatment was analyzed in 5 replicates.

In addition, referring to Figures 7a and 7b, the outdoor cabbage field of the house condition was treated with ultrasonic waves of 30 kHz, 3.28 v and 70 dB conditions. There are a plurality of ultrasonic wave generators, and the ultrasonic wave generators looking at each other may be installed at a distance of 20m to 60m. The treatment time was from 6 pm until 8 am the next morning. The cabbage used for the test is a sambo ogle, germinated in mid-February, and formulated around March 10, and treated with pabam moths around March 27. The green beetle moths were 8 per abandon, with 6 repetitions.

Pesticides, that is, drug treatment was performed using Bacillus thuringiensis, and was prepared at 200 ppm and treated with 3 times of ingot method. Chinese cabbage moths occurred 15 per abandon and treated with 3 repetitions of the ingot method. The Bt treatment was the same as that of the beech moth control treatment.

ANOVA analysis was performed using SAS 'PROC GLM (SAS Institute, 1989). The data were plotted using Sigma Plot 8.0 (Systat Software, Inc., Point Richmond, CA, USA).

The effects of sonication on larval feeding activity are as follows. Referring to Figure 1, it was analyzed the effect of the ultrasonic larvae feeding behavior of the five night insects. Species of beetroot moths are kept at 25 ° C. for 5 days and proceed to liquefaction. At this time, the feeding proceeds for the first three days, and the feeding decreases from the fourth day, causing the roaming behavior to fall from the food on the fifth day. Therefore, feeding analysis was performed using 5 worms on day 2, and the feeding amounts were compared in different exposures to ultrasound for 24 hours. At this time, the feeding amount decreased as the frequency of the ultrasound increased, and the feeding amount was reduced by more than 50% especially at 30 kHz and 45 kHz treatment.

Analysis of the biomaterial change by the ultrasonic treatment is as follows. Referring to Figure 2a to 2c, as a result of analyzing the change in the total protein (protein) content present in Pabam moth plasma according to the ultrasonic treatment, the protein content of about 4% was constant up to 30 kHz regardless of frequency. But above 35 kHz, plasma protein content was observed to decrease. Lipids in the plasma were about 0.3% in the control group, but increased significantly in all the ultrasonic treatment groups, and about 1.3% in the larvae treated with 30 kHz. But again, treatments above 35 kHz tended to decrease lipid content. In the case of free sugar present in the plasma, about 0.15% was shown, but the blood glucose was increased about 0.5% with the sonication.

3A to 3D, gene expression patterns of three types of heat shock proteins and lipid transport proteins, ApoLpIII, are shown by ultrasonic treatment. All four genes examined expressed in control. In the case of hsp70, the expression level was sensitive to sonication. However, due to the different sensitivity of tissues, the expression of blood cells decreased in all sonic treatments, and in the case of fat body, the weak expressions of control were maintained. In addition, in the case of epidermal cells, the expression level tended to decrease significantly above 30 kHz. hsp74 inhibited the expression of all three tissues in all sonications. In the case of hsp83, the amount of expression was decreased by sonication. ApoLpIII was relatively unresponsive to sonication, but epidermal cells tended to decrease somewhat.

In addition, referring to Figure 4, there is shown a change in the growth for the night moth according to the ultrasonic treatment. As the frequency of ultrasound treated increased, the dragon development period was delayed. In general, the growth period of about 5 days at 25 ℃ was increased to about 8 days when treated with 45 kHz ultrasound. In addition, the growth rate was significantly reduced due to adulthood, and the allegory rate was reduced to 30% when treated with 45 kHz.

In addition, referring to Figures 5a to 5c, in order to analyze the effect of the ultrasonic wave on the adult physiological phenomenon is shown the mating rate and the scattering force of the beech moth by processing various ultrasonic waves. The mating of the beech moth was identified based on the presence and number of spermatophore present in the female dorsum sac (see FIG. 5A). Referring to FIG. 5B, when one female and two males are kept in a 0.2 L container used in the present invention, the number of sperm sacs present in the copulation sacs of the treated females is determined to have been mated about two or more times for four days. It was. The number of copulations decreased with sonication, and when the treatment was 45 kHz, the average number of copulations decreased by about 10 times. That is, in the case of the control group, 2.3, 20 kHz sonication, 1.15, 45 kHz sonication shows a value of 0.15, and it is understood that the rate of mating is reduced by 1/2 to 1/15 times when sonication is performed.

In addition, referring to FIG. 5C, the female showed a decrease in the number of ovipositions during the sonication. About 780 eggs were laid for the control, while up to 200 eggs were spawned when treated with 45 kHz.

Referring to FIG. 6, a configuration diagram of an ultrasonic wave generator 600 used in the present invention is shown. The ultrasonic wave generator 600 may include an ultrasonic wave generator 610, a power supply unit 620, a device support unit 630, a timer 640, an operation controller 650, and a control unit 660.

The ultrasonic generator 610 may generate ultrasonic waves in various principles. For example, the ultrasonic generator 610 may generate ultrasonic waves by converting a high frequency alternating current into mechanical vibration by a reverse piezoelectric effect.

The power supply unit 620 supplies power required for the ultrasonic wave generator 600, and the current may be in the form of alternating current or direct current.

The device support 630 may be a component for coupling the ultrasonic generator 600 to a specific facility, for example, the interior of the facility house. For example, the device support 630 may include a coupling structure of a bolt and a nut, and the ultrasonic wave generator 600 may be coupled to the frame of the vinyl house by a coupling structure of the bolt and the nut.

The timer 640 may be provided to time the ultrasonic generation operation, and the operation controller 650 may control the operation time of the ultrasonic generator 610 using the time value of the timer 640.

In this case, the ultrasonic generator 610 may continuously generate ultrasonic waves of the same frequency to the plants or periodically change ultrasonic waves of different frequencies to generate the plants. In the latter case, since the effects of the ultrasonic waves of different frequencies described herein can be provided to the plant in various ways, there is an excellent pest control effect on the plant. For example, the ultrasonic generator 610 may generate ultrasonic waves of 30 kHz or 45 kHz according to a predetermined cycle by using the time value of the timer 640. In this case, the predetermined period may be 1 hour, 2 hours, 4 hours, 6 hours, 12 hours, 24 hours, and the like, and the period may be changed by a user's setting of the predetermined period setting unit. .

The control unit 660 may be configured such that the above-described information ultrasonic generator 610, the power supply unit 620, the device support unit 630, the timer 640, and the operation control unit 650 interoperate with each other to perform their own functions. Control each functional unit.

In addition, referring to FIG. 7C, the insecticidal insects of about 25% after 5 days of treatment with Bt (200 ppm) were treated with pabam moths (95% or more of the larvae and the remaining four-year-old larvae) in the outdoor packaging. . On the other hand, ultrasonic treatment showed about 35% insecticidal activity, and Bt and ultrasonic treatment together showed a high insecticide rate of about 60%. Here, the frequency of the ultrasonic wave may be any one of 30 kHz to 45 kHz.

In addition, referring to Figure 7d, the case of the Chinese cabbage moth also shows the results of analyzing the control effect on most species. Treatment with Bt (200 ppm) alone showed about 34% insecticide after 5 days of treatment, while ultrasound treatment showed about 37% insecticide, and when Bt and ultrasound were treated together, it was about 80% higher. It was shown to exhibit control efficiency. Here, the frequency of the ultrasonic wave may be any one of 30 kHz to 45 kHz.

Insect physiological changes to stress sound waves have progressed for Pabamoth moths. High frequency (particularly 5,000 Hz) in the analyzed insects inhibited developmental physiology, feeding behavior and spawning physiology. The present invention analyzed the effect of ultrasound on the physiological disturbances of Pabam moth.

Ultrasound reduced the larva's feeding behavior. Especially at 30 kHz and 45 kHz, more than 50% of the feeding behaviors were suppressed. It also caused changes in nutrients in the plasma. The sonication decreased the protein content but increased the blood glucose and lipid concentrations. The percentage of nutrients present in the untreated P. vulgaris larvae plasma was the most abundant protein, followed by lipids and blood glucose. This change in the content of nutrients in the plasma may be due to the sonication acts as a stress on the beech moth. The major hormone secreted by insects in connection with stress is adipokinetic hormone (AKH).

AKH, which raises lipids and blood sugar, helps to cope with stress by causing physiological changes that increase catabolism. When Pabam moths are exposed to stress sound waves, the endocrine organs, corpora cardiaca (CC), secrete AKH and promote the catabolism of fats, which are organs of this hormone, to promote the catabolism of diglycerides in stored triglycerides or glycogens. Glucose is released, and glucose is converted back into insect blood trehalose, which is released into the hemolymph. As a result, Pabam moths exposed to stress sound waves are converted into a stress coping state in which the lipid content of plasma and the concentration of free sugar are increased. More than 30 kHz of sonication causes a decrease in the content of proteins, which can result in effects due to lower overall metabolism.

Degradation in physiological coping with ultrasound stress is associated with suppression of stress-related gene expression. That is, heat shock protein (HSP) is a stress-related protein expressed by factors such as high temperature, low temperature, dryness, and nutritional deficiency. Their original function is to play a role in chaperones, which are involved in protein synthesis in the cell and normalize the tertiary structure of proteins. These thermal shock proteins are generally classified into four classes, including small HSPs having a molecular weight of 20-30 kDa, HSP60 having a molecular weight of 60 kDa, HSP70 having a 70 kDa, and HSP83 having 83 kDa.

In the present invention, three hsp genes identified in Pabam moths were analyzed for expression by ultrasonic treatment. In all three tissues, gene expression of all heat shock proteins was inhibited by sonication. Inhibition of the expression of hsp genes by the sonication causes a physiological adverse effect that Pabam moth loses its ability to cope with stress. Stress sensitivity for ultrasound was also shown in ApoLpIII gene expression. ApoLpIII is a member of the lipid transport protein, which binds lipid protein to high density lipophorin, a plasma lipid transporter of insects composed of relatively high molecular weight ApoLp I and II, and thus, firmly binds lipid protein binding when converted to low density lipophorin. It will play the role of letting them go.

ApoLpIII also functions as an immune material that recognizes lipopolysaccharides present in the outer membrane of bacteria. Inhibiting the expression of ApoLpIII by RNA interference in Pabam moth greatly reduces immunity and development is inhibited. Inhibition of expression of this protein by ultrasound can lead to decreased lipid transport capacity and reduced immunity. In particular, the transport of elevated lipids into the plasma following sonication is less likely to contribute to their immunity, leading to less immune coping deficiency.

In addition, the somatic treatment inhibited the development of the dragon and adult mating behavior. Chestnut moths have been shown to detect ultrasound through the tympanic organ in the first section of the abdomen. The avoidance behavior for ultrasound is programmed in these insects as reflex motion, and the ultrasound received by the tympanic organ is detected by bat recognition and then connected to the motor nerve.

In addition, the method for controlling pests using ultrasonic waves according to an embodiment of the present invention and a detailed device configuration diagram for the system, an interface standardization technology such as an embedded system, O / S, communication protocols, I / O interfaces, and actuators Details of parts standardization technology, such as a battery, a camera, and a sensor, will be omitted since they are obvious to those skilled in the art.

The pest control method using ultrasonic waves according to the present invention can be implemented in the form of program instructions that can be executed by various computer means and recorded in a computer readable medium. That is, the recording medium may be a computer-readable recording medium having recorded thereon a program for causing a computer to execute the steps described above.

The computer readable medium may include a program command, a data file, a data structure, etc. alone or in combination. The program instructions recorded on the medium may be those specially designed and constructed for the present invention or may be available to those skilled in the art of computer software. Examples of the computer-readable recording medium include magnetic media such as a hard disk, a floppy disk and a magnetic tape, optical recording media such as CD-ROM and DVD, magnetic recording media such as a floppy disk Optical media, and hardware devices specifically configured to store and execute program instructions such as ROM, RAM, flash memory, and the like.

The medium may be a transmission medium such as an optical or metal line, a wave guide, or the like, including a carrier wave for transmitting a signal designating a program command, a data structure, or the like. Examples of program instructions include not only machine code generated by a compiler, but also high-level language code that can be executed by a computer using an interpreter or the like. The hardware devices described above may be configured to operate as one or more software modules to perform the operations of the present invention.

In addition, each of the above-described components may be implemented in one physically adjacent component or may be implemented in different components. In the latter case, each component may be controlled by being located adjacent or in different zones. In this case, the present invention is provided with a separate control means or control room for controlling each component to control each component by wire or wirelessly. You may.

In the above description, the respective components and / or functions described in the embodiments may be combined and combined, and those skilled in the art will understand that the present invention It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

600: ultrasonic generator 610: ultrasonic generator
620: power supply 630: device support
640: timer 650: operation control unit
660: control unit

Claims (9)

Treating the medicament on the plantation of the plant; And
An ultrasonic generator for generating an ultrasonic wave of a specific frequency includes the step of generating the ultrasonic wave in the plantation of the plant to increase the efficiency of the drug control against the beetroot moth,
The frequency of the ultrasonic wave is 30 kHz or 45 kHz pest control method using ultrasonic waves, characterized in that.
delete The method according to claim 1,
The intensity of the ultrasonic wave is 70 to 95 dB pest control method using ultrasonic waves, characterized in that.
The method according to claim 1,
The ultrasonic generator is a pest control method using the ultrasonic wave, characterized in that installed in the facility house.
delete The method according to claim 1,
The ultrasonic generating device is a plurality, the ultrasonic generating device to look at each other is pest control method using ultrasonic waves, characterized in that installed 20m to 60m apart.
The method according to claim 1,
The ultrasound is characterized by suppressing the feeding activity of the pest, varying the content of nutrients in the plasma, increasing blood sugar, inhibiting the development of the dragon, extending the container, and lowering the rate of allegory to adult Pest control method using.
The method according to claim 1,
The ultrasonic wave is a pest control method using ultrasonic waves, characterized in that to reduce the mating rate of the pests 1/2 times to 1/15 times.
The method according to claim 1,
The ultrasonic generator is a pest control method using the ultrasonic wave, characterized in that to periodically change the ultrasonic waves of different frequencies generated in the plantation of the plant.

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