KR101268184B1 - Manufacturing Method Traditional Brewed Alcoholic Beverage - Google Patents

Manufacturing Method Traditional Brewed Alcoholic Beverage Download PDF

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KR101268184B1
KR101268184B1 KR1020110056101A KR20110056101A KR101268184B1 KR 101268184 B1 KR101268184 B1 KR 101268184B1 KR 1020110056101 A KR1020110056101 A KR 1020110056101A KR 20110056101 A KR20110056101 A KR 20110056101A KR 101268184 B1 KR101268184 B1 KR 101268184B1
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weight
hours
glutinous rice
rice
parts
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KR20120136902A (en
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김용두
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순천시
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/021Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn
    • C12G3/022Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn of botanical genus Oryza, e.g. rice
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12HPASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/02Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages combined with removal of precipitate or added materials, e.g. adsorption material
    • C12H1/06Precipitation by physical means, e.g. by irradiation, vibrations
    • C12H1/061Separation by centrifugation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12HPASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/16Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation by physical means, e.g. irradiation
    • C12H1/18Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation by physical means, e.g. irradiation by heating
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12HPASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/22Ageing or ripening by storing, e.g. lagering of beer

Abstract

본 발명은 찹쌀을 씻어 4~6시간 물에 침지한 후에 4~6시간 탈수하여 준비하는 원료 세척 및 침지 단계; 준비된 찹쌀을 분쇄하고, 물과 탁주용 정제효소를 첨가하여 죽을 제조한 후 실온으로 냉각하는 죽 제조 단계; 냉각시킨 죽에 밀가루 누룩, 효모액, 젖산을 첨가하고, 72시간 동안 23℃로 발효시켜 주모를 제조한 후 5℃로 냉각하는 주모 제조 단계; 제조된 주모에 증자한 찹쌀로 제조한 고두밥, 물, 밀가루 누룩, 탁주용 정제효소를 첨가하고, 25℃에서 12시간 간격으로 교반하여 3일(72시간)간 발효시키는 1단 담금 단계; 1단 담금된 술덧에 증자한 찹쌀로 제조한 고두밥, 물, 탁주용 정제효소를 첨가하고 25℃에서 12일(288시간)간 발효시키는 2단 담금 단계;를 포함하는 전통 발효주 제조 방법에 관한 것이다.The present invention is a raw material washing and immersion step of washing the glutinous rice 4 to 6 hours after immersion in water to prepare for 4 to 6 hours dehydration; Grind the prepared glutinous rice, and add the water and Takju purified enzyme to prepare the porridge and then the porridge production step of cooling to room temperature; Flour nuruk, yeast solution, lactic acid is added to the cooled porridge, fermented at 23 ℃ for 72 hours to prepare a cast hair and then cooled to 5 ℃; 1 step immersion step of fermented for 3 days (72 hours) by adding a gourd rice, water, flour malt, Takju purified enzyme, prepared by the glutinous rice cooked with the cooked rice, and stirred at 25 ° C for 12 hours; It relates to a traditional fermented liquor manufacturing method comprising; a two-stage immersion step of fermented for 12 days (288 hours) at 25 ° C. by adding Godu rice, water, and refined tablets made with glutinous rice, which was added to the sweetened rice wine. .

Description

전통 발효주 제조 방법 {Manufacturing Method Traditional Brewed Alcoholic Beverage}Manufacturing Method Traditional Brewed Alcoholic Beverage

본 발명은 전통 발효주 제조 방법에 관한 것으로, 구체적으로는 원료 세척 및 침지 단계, 죽 제조 단계, 주모 제조 단계, 1단 담금 단계, 2단 담금 단계, 청징 및 냉장 단계를 거쳐 전통 발효주를 제조하는 방법에 관한 것이다. The present invention relates to a method for producing traditional fermented liquor, specifically, a method for preparing traditional fermented liquor through raw material washing and soaking step, porridge manufacturing step, coriander preparation step, one-stage immersion step, two-stage immersion step, clarification and refrigeration step. It is about.

우리나라의 전통주인 발효주는 미생물에 의해 탄수화물이 분해되면서 알코올을 비롯한 여러 가지 성분이 생기는 일종의 발효 음료이며, 발효원으로는 전분을 주성분으로 하는 곡류, 감자류와 당분을 주성분으로 하는 과일, 당밀 등이 이용된다. Fermented liquor, a traditional Korean liquor, is a kind of fermented beverage in which carbohydrates are broken down by microorganisms to produce various components such as alcohol. do.

발효주로서의 약·탁주는 전통적으로 곡류와 누룩을 사용하여 병행복발효로 제조되며, 양조 후에 술덧을 체로 걸러서 외관이 백탁한 것은 막걸리 또는 탁주라 하고, 술덧에 용수를 받아서 맑은 액만 취한 것을 약주라 한다. Medicine and Takju as fermented liquor are traditionally produced in parallel fermentation using grains and yeast, and it is called makgeolli or Takju after brewing sifting after brewing. .

일반적으로 담금 후, 누룩 중의 미생물에 의한 효소작용으로 원료성분이 분해, 발효되어 알코올 농도가 12-15%가 되며, 이것을 거를 때 물을 첨가하여 알코올 농도 6-8%의 탁주를 제조한다. 탁주는 비타민 B군을 비롯한 필수아미노산인 lysine, leucine, glutamic acid, proline 및 glutathion을 함유하여 영양가가 풍부하며, 산미에 관여하는 유기산은 갈증을 해소하고 신진대사를 원활히 하는데 효과가 있다. In general, after soaking, the raw material is decomposed and fermented by enzymatic action by microorganisms in the yeast, and the alcohol concentration is 12-15%. When this is filtered, water is added to prepare a alcoholic beverage having 6-8% alcohol concentration. Takju contains nutrients such as vitamin B group, essential amino acids lysine, leucine, glutamic acid, proline and glutathion, which are rich in nutrition. Organic acids involved in acidity are effective in quenching thirst and smoothing metabolism.

우리나라의 술은 일제시대부터 100여 년간 자유로운 제조가 허락되지 않은 연유로 전통주의 산업화와 전통주에 관한 연구가 매우 빈약하다. 그러나, 최근 한국 문화의 세계화 추세와 한류의 영향으로 국내외적으로 전통 민속주에 대한 관심이 높아지고 있으며, 민속주를 복원하려는 노력이 시도되고 있다. The liquor of our country has been poorly studied for the industrialization of traditionalism and traditional liquor since it was not allowed to be freely manufactured for over 100 years. However, due to the recent trend of globalization of Korean culture and the influence of the Korean Wave, there has been a growing interest in traditional folk liquor at home and abroad, and efforts have been made to restore folk liquor.

기존의 전통 발효주는 구전되거나 가정용으로만 주조되어 표준화된 제조방법이 없고, 전통 발효주의 맛과 풍미를 결정하는 맛, 향, 색 및 영양 발현에 대한 개별적인 고려가 없어 단일한 맛과 저장 기간을 보장하는 전통주의 제조가 어려운 실정이다. Existing traditional fermented liquor has no oral or home-brewed standardized manufacturing methods, and there is no individual consideration of the taste, aroma, color, and nutritional expressions that determine the taste and flavor of traditional fermented liquor, ensuring a single taste and shelf life. It is difficult to manufacture traditionalism.

위와 같은 문제를 해결하기 위한 것으로 본 발명은 맛, 향, 색 등이 일정한 전통 발효주의 표준 규격화된 제조 방법을 제공하는데 목적이 있다.In order to solve the above problems, the present invention has an object to provide a standardized manufacturing method of the traditional fermented wine is a constant taste, aroma, color and the like.

또한, 종래의 전통 발효주보다 개선된 맛, 향, 색 및 영양을 발현할 수 있는 전통 발효주의 제조 방법을 제공하는데 목적이 있다.In addition, an object of the present invention is to provide a method for preparing traditional fermented wine which can express improved taste, aroma, color and nutrition than traditional fermented wine.

위와 같은 목적을 달성하기 위하여 본 발명에서는 찹쌀을 씻어 4~6시간 물에 침지한 후에 4~6시간 탈수하여 준비하는 원료 세척 및 침지 단계; 준비된 찹쌀을 분쇄하고, 물과 탁주용 정제효소를 첨가하여 죽을 제조한 후 실온으로 냉각하는 죽 제조 단계; 냉각시킨 죽에 밀가루 누룩, 효모액, 젖산을 첨가하고, 72시간 동안 23℃로 발효시켜 주모를 제조한 후 5℃로 냉각하는 주모 제조 단계; 제조된 주모에 증자한 찹쌀로 제조한 고두밥, 물, 밀가루 누룩, 탁주용 정제효소를 첨가하고, 25℃에서 12시간 간격으로 교반하여 3일(72시간)간 발효시키는 1단 담금 단계; 1단 담금된 술덧에 증자한 찹쌀로 제조한 고두밥, 물, 탁주용 정제효소를 첨가하고 25℃에서 12일(288시간)간 발효시키는 2단 담금 단계;를 포함하는 전통 발효주 제조 방법을 제공한다.In order to achieve the above object, in the present invention, the raw material washing and immersion step of washing the glutinous rice to be dehydrated for 4-6 hours after immersion in water for 4-6 hours; Grind the prepared glutinous rice, and add the water and Takju purified enzyme to prepare the porridge and then the porridge production step of cooling to room temperature; Flour nuruk, yeast solution, lactic acid is added to the cooled porridge, fermented at 23 ℃ for 72 hours to prepare a cast hair and then cooled to 5 ℃; 1 step immersion step of fermented for 3 days (72 hours) by adding a gourd rice, water, flour malt, Takju purified enzyme, prepared by the glutinous rice cooked with the cooked rice, and stirred at 25 ° C for 12 hours; It provides a traditional fermented liquor manufacturing method comprising; a two-stage immersion step of fermented at 25 ℃ for 12 days (288 hours) by adding Godu rice, water, Takju purified enzyme prepared in the glutinous rice, steamed in one step soaked .

이때, 상기 죽 제조 단계는 분쇄된 찹쌀 400g당, 물 800mL, 찹쌀 100중량부 기준으로 0.13~0.17중량부인 탁주용 정제효소를 첨가하여 제조하며, 상기 주모 제조 단계는 제조된 죽 1.2kg 기준으로, 밀가루 누룩 400g, 효모액 50mL, 주모 100중량%에 0.3~0.7중량% 포함되는 젖산을 첨가하여 제조한다.At this time, the porridge manufacturing step is prepared by adding a tablet enzyme for Takju 0.13 ~ 0.17 parts by weight based on 400g of crushed glutinous rice, 800mL of water, 100 parts by weight of glutinous rice, the main hair manufacturing step is based on the prepared porridge 1.2kg, It is prepared by adding lactic acid, which contains 0.3-0.7% by weight to 400g of wheat yeast, 50mL of yeast solution, and 100% by weight of hair.

또한, 상기 1단 담금 단계는 제조된 주모 1L당, 증자한 찹쌀 2kg을 고두밥으로 제조한 것, 물 2.6L, 찹쌀 100중량부 기준으로 9~11중량부의 밀가루 누룩, 0.13~0.17중량부의 탁주용 정제효소를 첨가하여 제조하며, 상기 2단 담금 단계는 1단 담금된 술덧 5.8kg당, 증자한 찹쌀 8kg을 고두밥으로 제조한 것, 물 12L, 찹쌀 100중량부의 0.2~0.4중량부인 탁주용 정제효소를 첨가하여 제조한다. 이때, 상기 주모 제조 단계에서 효모액으로 Saccharomyces cerevisiae KCCM 32021를 사용한다.In addition, the one-stage immersion step is produced per 1L of the prepared rice wine, cooked glutinous rice 2kg with gourd rice, water 2.6L, wheat flour yeast based on 100 parts by weight of glutinous rice, 0.13 ~ 0.17 parts by weight for takju Prepared by the addition of purified enzyme, wherein the two-stage immersion step is 5.8kg per 1 soaked glutinous rice, 8kg of cooked glutinous rice made with soybean rice, 12L of water, 100 parts by weight of glutinous rice 0.2 ~ 0.4 parts by weight of tablet enzyme for Takju It is prepared by adding. At this time, Saccharomyces cerevisiae KCCM 32021 is used as the yeast solution in the step of preparing the main hair.

또한, 상기 주모 제조 단계에서 첨가되는 상기 밀가루 누룩은 밀가루 100중량부에 30중량부의 정제수를 첨가하여 110℃에서 15분간 고압증기로 살균하고, 종국(seed koji)으로 제조된 균주를 성형없이 30℃에서 24시간 1차 배양하며, 1차 배양한 밀가루 누룩을 성형한 후 30℃, 습도 85%에서 48시간 2차 배양하고, 30℃에서 수분함량이 9~11%가 되게 건조하여 사용한다.In addition, the wheat flour yeast added in the step of preparing the main hair is sterilized by high-pressure steam at 110 ℃ for 15 minutes by adding 30 parts by weight of purified water to 100 parts by weight of wheat flour, 30 ℃ without molding the strain prepared in (seed koji) After primary culture at 24 hours, the primary cultured flour yeast was molded and then secondary cultured at 30 ° C. and humidity at 85% for 48 hours, and dried at 30 ° C. to have a water content of 9-11%.

또한, 2단 담금한 술덧을 3000rpm으로 30분간 원심분리하여 청징한 후, 1~5℃로 냉장 보관하는 청징 및 냉장 단계를 더 포함하며, 병입하여 중탕으로 80℃에서 10분간 열처리하는 살균 단계를 더 포함한다.In addition, the step immersed in two stages quenched by centrifugation for 30 minutes at 3000rpm, and further comprises a clarification and refrigeration step of refrigeration at 1 ~ 5 ℃, bottling step sterilization step of heat treatment at 80 ℃ in a bath 10 minutes It includes more.

특히, 상기 1단 담금 단계, 2단 담금 단계에서, 상기 고두밥은 100중량% 기준으로 찹쌀이 70~75중량%, 수분이 25~30중량% 포함되도록 제조한다. In particular, in the one-stage immersion step, the two-stage immersion step, the soybean rice is prepared to include 70 to 75% by weight, 25 to 30% by weight of glutinous rice based on 100% by weight.

본 발명의 전통 발효주 제조 방법에서는 표준 규격화된 제조 방법을 제공하여 전통 발효주의 맛, 향, 색 등이 일정한 장점이 있다.Traditional fermented liquor manufacturing method of the present invention provides a standardized manufacturing method has a certain advantage, such as taste, flavor, color, etc. of traditional fermented liquor.

또한, 종래의 전통 발효주보다 개선된 맛, 향, 색 및 영양을 발현할 수 있는 전통 발효주의 제조 방법을 제공하는데 목적이 있다.In addition, an object of the present invention is to provide a method for preparing traditional fermented wine which can express improved taste, aroma, color and nutrition than traditional fermented wine.

도 1은 본 발명의 전통 발효주 제조 순서를 보여주는 개략적인 순서도이다. Figure 1 is a schematic flow chart showing a traditional fermented liquor manufacturing sequence of the present invention.

이하 첨부된 도면을 참조하면서 본 발명에 따른 바람직한 실시 예를 상세히 설명하기로 한다. 이에 앞서, 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여, 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately The present invention should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention.

따라서, 본 명세서에 기재된 실시 예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시 예에 불과할 뿐이고 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있음을 이해하여야 한다.
Therefore, the embodiments described in the specification and the drawings shown in the drawings are only the most preferred embodiments of the present invention and do not represent all of the technical idea of the present invention, various modifications that can be substituted for them at the time of the present application It should be understood that there may be equivalents and variations.

본 발명에서는 도 1에 도시된 바와 같이 원료 세척 및 침지 단계, 죽 제조 단계, 주모 제조 단계, 1단 담금 단계, 2단 담금 단계, 청징 및 냉장 단계를 거쳐 전통 발효주를 제조한다.
In the present invention, as shown in Figure 1 through the raw material washing and immersing step, porridge manufacturing step, casting step manufacturing step, one-stage immersion step, two-stage immersion step, clarification and refrigeration step to prepare a traditional fermented wine.

원료 세척 및 침지 단계Raw material washing and dipping step

먼저, 전통 발효주의 재료인 찹쌀 400g을 깨끗하게 씻어 4~6시간 물에 침지한 후에 다시 4~6시간 정도 물이 빠지도록 한다.
First, wash 400g of glutinous rice, a traditional fermented wine, and soak it in water for 4-6 hours, and then drain the water for 4-6 hours.

죽 제조 단계Porridge manufacturing steps

세척 및 침지를 마친 찹쌀 400g을 분쇄하고, 이에 물 800mL, 찹쌀 100중량부를 기준으로 당화제 0.13~0.17중량부를 첨가하여 죽을 제조하고, 실온과 동일해질 때까지 냉각하며, 이때, 상기 당화제는 찹쌀 100중량부를 기준으로 0.15중량부(찹쌀 400g당 0.6g)를 첨가하는 것이 바람직하다. 400 g of glutinous rice, which has been washed and immersed, is pulverized, and rice porridge is prepared by adding 0.13 to 0.17 parts of saccharifying agent based on 800 mL of water and 100 parts by weight of glutinous rice, and cooled until the same as room temperature, wherein the saccharifying agent is glutinous rice. It is preferable to add 0.15 parts by weight (0.6 g per 400 g of glutinous rice) based on 100 parts by weight.

상기 당화제로는 쌀 코지(koji, 국)와 옥수수 전분 코지 및 탁주용 정제효소, 누룩을 이용할 수 있으나, 탁주용 정제효소를 사용한 경우의 알코올 함량이 가장 높고, 향, 색, 맛을 기준으로 한 관능평가 결과에서도 가장 좋은 점수를 얻었는 바, 당화제로 탁주용 정제효소를 사용하는 것이 바람직하다. The saccharifying agent may be rice koji (koji, soup) and corn starch koji and Takju tablet enzyme, yeast, but the highest alcohol content when using Takju tablet enzyme, based on the flavor, color, taste In the sensory evaluation result, the best score was obtained. Therefore, it is preferable to use Takju purified enzyme as a saccharifying agent.

아래 표 1에는 당화제의 종류에 따른 알코올 함량 및 관능평가 결과가 나타나 있다.Table 1 below shows the alcohol content and sensory evaluation results according to the types of saccharifying agents.

구분division 알코올(%)Alcohol(%) incense color flavor 쌀 kojiRice koji 16.0116.01 3.93.9 4.04.0 4.04.0 옥수수전분 kojiCorn starch koji 15.8415.84 3.93.9 3.93.9 3.83.8 탁주용 정제효소Takju purified enzyme 16.1216.12 4.14.1 4.14.1 4.24.2 누룩yeast 15.9715.97 4.04.0 4.04.0 4.14.1

상기 탁주용 정제효소는 역가 15,000 SP/g이고, 성분은 효소분말 1(국 정제효소, 당화력역가 100,000sp)이 6중량%, 효소분말 2(국 글루코아밀라아제, 당화력역가 50,000sp)가 18중량%, 황산칼슘 20중량%, 탄산칼슘 56중량%로 구성된다.
The Takju purified enzyme has a titer of 15,000 SP / g, the component is 6% by weight of enzyme powder 1 (national purified enzyme, glycosylation potency 100,000sp), 18% by weight of enzyme powder 2 (national glucoamylase, 50,000 g of glycosylation potency). 20 wt% calcium sulfate, 56 wt% calcium carbonate.

주모 제조 단계Main hair manufacturing steps

냉각 시킨 죽에 밀가루 누룩 400g, 효모(Saccharomyces SP 32021)액 50mL, 주모 100중량%당 0.3~0.7중량%가 되도록 젖산을 첨가한 후, 3일간 23℃로 발효하여 주모를 제조하고 5℃에 보관하면서 사용한다. 이때, 상기 젖산은 주모 100중량%당0.5중량%인 것이 바람직하다.
After cooling the porridge, lactic acid was added to 400 g of wheat yeast, 50 mL of yeast (Saccharomyces SP 32021) solution, and 0.3 to 0.7% by weight per 100% by weight of fermented rice, followed by fermentation at 23 ° C. for 3 days. Use it while. At this time, the lactic acid is preferably 0.5% by weight per 100% by weight of the main hair.

상기 밀가루 누룩은 밀가루 100중량부에 30중량부인 정제수를 첨가하여 110℃에서 15분간 고압증기살균하고, 종국(seed koji)으로 제조된 Aspergillus kawachii KCCM 32819, Aspergillus niger KCCM 32005, Rhizopus japonicus KCCM 11604, Rhizopus oryzae KCCM 11272, Rhizopus oryzae KCCM 11273, Rhizopus oryzae KCCM 11276, Mucor rouxii KCCM 60148 중 하나를 단독 접종하여 성형 없이 30℃에서 24시간 1차 배양한다. 1차 배양한 밀가루 누룩을 성형한 후 30℃, 습도 85%에서 48시간 2차 배양한 다음 30℃에서 수분함량이 10%내외(9~11%)가 되게 건조시켜 사용한다.
The wheat flour yeast was added to 100 parts by weight of wheat flour and 30 parts by weight of purified water and autoclaved at 110 ° C. for 15 minutes, and finally (seed koji) Aspergillus kawachii KCCM 32819, Aspergillus niger KCCM 32005, Rhizopus japonicus KCCM 11604, Rhizopus One of oryzae KCCM 11272, Rhizopus oryzae KCCM 11273, Rhizopus oryzae KCCM 11276, Mucor rouxii KCCM 60148 was inoculated alone and incubated for 24 hours at 30 ° C without molding. After the first cultured wheat flour Nuruk was incubated for 2 hours at 30 ℃, 85% humidity for 48 hours and then dried to 30% moisture content of about 10% (9 ~ 11%).

아래 표 2는 각각의 균주별로 당화력을 측정한 결과로서 상위 4개의 균주에 대한 것이다.Table 2 below is for the top four strains as a result of measuring the glycosylation power of each strain.

균주명Strain name 당화력(SP/g)Glucose Power (SP / g) Rhizopus oryzae KCCM 11272 Rhizopus oryzae KCCM 11272 3647.72 3647.72 Rhizopus japonicus KCCM 11604 Rhizopus japonicus KCCM 11604 3551.903551.90 Rhizopus oryzae KCCM 11273 Rhizopus oryzae KCCM 11273 3287.863287.86 Rhizopus oryzae KCCM 11276 Rhizopus oryzae KCCM 11276 2832.782832.78

또한, 전분의 액화제로 작용하는 a-amylase의 활성으로 누룩의 액화력을 파악할 수 있는데, Rhizopus japonicus KCCM 11604로 제조한 누룩의 활성이 가장 높다.
In addition, the liquefaction power of yeast can be determined by the activity of a-amylase, which acts as a liquefaction agent of starch. The activity of yeast prepared by Rhizopus japonicus KCCM 11604 is the highest.

상기 주모 제조시 첨가되는 효모액은 누룩 50g에 증자한 쌀 100g, 물 150mL를 혼합한 후 일정비율로 배양하여 사용하는데, Saccharomyces cerevisiae KCCM 32021를 사용하는 것이 바람직하다. Saccharomyces cerevisiae KCCM 32021, Saccharomyces cerevisiae KCCM 11306, Saccharomyces kluyveri, 탁주효모, Saccharomyces bayanus, Saccharomyces fumivin, Saccharomyces cerevisiae KCCM 11290와 같은 효모들을 주모 제조시에 첨가하고 20℃에서 발효시켜 발효특성을 평가한 결과, pH 변화, 산도, 환원당 함량 변화, 알코올 함량 변화, 관능평가(향, 색, 맛)에서 Saccharomyces cerevisiae KCCM 32021로 담금 한 경우의 결과가 가장 좋다. 구체적으로 pH는 담금 후 12일째에 Saccharomyces cerevisiae KCCM 32021가 4.2로 가장 높고, 총산 함량 역시 Saccharomyces cerevisiae KCCM 32021로 담금 한 주모가 0.93%로 가장 높으며, 환원당 함량은 3.07 ~ 4.2%로 최저 수준이다. 최종 알코올 함량은 Saccharomyces cerevisiae KCCM 32021가 18.5%로 가장 높고, 관능평가 결과 향, 색, 맛에서 Saccharomyces cerevisiae KCCM 32021로 담금 한 주모의 점수가 높다.
The yeast solution added during the preparation of the main hair is used by incubating at a certain ratio after mixing 100 g of rice and 150 mL of water, which is added to 50 g of leaven, and it is preferable to use Saccharomyces cerevisiae KCCM 32021. Yeasts such as Saccharomyces cerevisiae KCCM 32021, Saccharomyces cerevisiae KCCM 11306, Saccharomyces kluyveri, Takju yeast, Saccharomyces bayanus, Saccharomyces fumivin, Saccharomyces cerevisiae KCCM 11290 were added during fermentation and fermentation at 20 ° C. The best results were obtained by immersion with Saccharomyces cerevisiae KCCM 32021 in the changes of acidity, acidity, reducing sugar content, alcohol content, and sensory evaluation (flavor, color, taste). Specifically, pH was highest in Saccharomyces cerevisiae KCCM 32021 at 4.2 on the 12th day after soaking, and total acid content was the highest at 0.93%, and the lowest content of reducing sugar was 3.07-4.2% in Saccharomyces cerevisiae KCCM 32021. The final alcohol content was highest in Saccharomyces cerevisiae KCCM 32021 (18.5%), and sensory evaluation showed that the score of the mother-in-law dipped in Saccharomyces cerevisiae KCCM 32021 was high in aroma, color and taste.

상기 젖산은 상기 주모에 첨가되는 농도에 따라서도 주모의 발효 특성을 변화시키는데, 본 발명에서는 분쇄한 찹쌀 1kg에 물 2L와 찹쌀 100중량부의 0.13~0.17중량부인 탁주용 정제효소를 첨가하여 죽을 제조한 후 냉각시켜 밀가루 누룩 1kg, 효모액(배양효모, 100g당 효모수 400억이상, 송천효모) 125mL를 첨가하고, 제조된 주모 100중량%에 대하여 0.3, 0.5, 0.7 및 1.0중량% 비율이 되도록 젖산을 첨가하여 주모를 제조하여 23℃에서 8일(192시간)간 발효시킨다. The lactic acid also changes the fermentation characteristics of the main hair according to the concentration added to the main hair, in the present invention, 1 kg of ground crushed glutinous rice and 100 parts by weight of 100 wt. After cooling, 1 kg of wheat flour yeast and 125 mL of yeast solution (cultivation yeast, more than 40 billion yeasts per 100 g, Songcheon yeast) were added, and lactic acid was added in an amount of 0.3, 0.5, 0.7, and 1.0% by weight based on 100% by weight of the prepared yeast. To prepare a main hair to add the fermentation at 23 ℃ 8 days (192 hours).

젖산이 주모 100중량%당 0.3중량% 이하인 경우에는 주모의 pH가 2일(48시간) 이후부터 급속히 감소하고 총산은 투입 시점보다 높은 수준에서 유지되는 반면, 젖산이 0.5중량% 이상인 첨가구는 담금 직후와 발효가 완료되는 8일째의 총산 함량이 차이를 보이지 않고, pH 역시 감소폭이 작은 상태로 일정하게 유지되므로 발효기간 동안 안정된 값을 갖는다. 또한, 에탄올(ethanol) 함량은 젖산 농도에 영향을 받지 않으며, 관능평가 결과는 젖산 0.5중량% 및 0.7중량% 첨가구의 기호도가 높아, 이러한 결과를 바탕으로 전통주 제조시 주모 100중량%에 대하여 0.5~0.7중량%의 젖산을 첨가하는 것이 바람직하다.
If lactic acid is 0.3 wt% or less per 100 wt% of the mother's hair, the pH of the mother's hair decreases rapidly after 2 days (48 hours) and the total acid is maintained at a higher level than the time of injection, while the addition of 0.5 wt% or more of lactic acid immediately after soaking The total acid content at 8 days after fermentation is complete does not show a difference, and pH also has a stable value during the fermentation period because the pH is kept constant with a small decrease. In addition, the ethanol content is not affected by the concentration of lactic acid, and the sensory evaluation result shows that the preference of 0.5 wt% and 0.7 wt% added lactate is 0.5 ~ 100 wt% based on the results of traditional liquor. Preference is given to adding 0.7% by weight of lactic acid.

1단 담금 단계 1st stage immersion stage

제조된 주모 1L에 증자한 찹쌀 2kg을 고두밥으로 제조한 것, 담금용수(물) 2.6L, 찹쌀 100중량부에 9~11중량부인 밀가루 누룩, 0.13~0.17중량부인 탁주용 정제효소를 첨가하고, 25℃에서 12시간 간격으로 교반하여 3일간 발효시킨다. 이때, 찹쌀(2kg) 100중량부에 10중량부인 200g의 밀가루 누룩, 0.15중량부인 3g의 탁주용 정제효소를 첨가하는 것이 바람직하다. 또한, 상기 고두밥은 찹쌀 70~75중량%, 수분 25~30중량%가 포함되도록 제조되는 것이 바람직하다.
1kg of cooked rice prepared with 2kg of glutinous rice cooked with gourd rice, 2.6L of immersion water (water), 100-11 parts of glutinous rice, 9-11 parts by weight of wheat yeast, 0.13-0.17 parts by weight of Takju purified enzyme, The mixture is stirred at 25 ° C for 12 hours and fermented for 3 days. At this time, it is preferable to add 200g wheat flour yeast, 10 parts by weight of 100g glutinous rice (2kg), 3g of ethanol tablet enzyme of 0.15 parts by weight. In addition, the gourd rice is preferably manufactured to include 70 to 75% by weight, 25 to 30% by weight of glutinous rice.

2단 담금 단계2-stage immersion stage

상기 1단 담금된 술덧(주료) 5.8kg 기준으로 증자한 찹쌀(찹쌀 중량 8kg)을 고두밥으로 제조한 것, 담금용수(물) 12L 및 찹쌀 100중량부에 0.2~0.4중량부인 탁주용 정제효소를 첨가하고 25℃에서 12일(288시간)간 발효시킨다. 이때, 상기 탁주용 정제효소는 상기 찹쌀 100중량부에 0.3중량부인 24g인 것이 바람직하다. 또한, 상기 고두밥은 찹쌀 70~75중량%, 수분 25~30중량%가 포함되도록 제조되는 것이 바람직하다.
Prepared glutinous rice (8 kg of glutinous rice weight) increased by the base of 5.8 kg of the soaked (soybean) in the first stage, made of gourd rice, 12-L of immersion water (water) and 0.2 to 0.4 parts by weight of Takju purified enzyme in 100 parts by weight of glutinous rice. Add and ferment at 25 ° C. for 12 days (288 hours). At this time, the Takju purified enzyme is preferably 24g of 0.3 parts by weight to 100 parts by weight of the glutinous rice. In addition, the gourd rice is preferably manufactured to include 70 to 75% by weight, 25 to 30% by weight of glutinous rice.

청징 및 냉장 단계Clarification and Refrigeration Steps

2단 담금한 술덧을 3000rpm로 30분간 원심분리하여 청징한 후, 1~5℃에서 냉장 보관한다.
After centrifugation and clarification for 30 minutes at 3000rpm in two stage immersion, and refrigerated at 1 ~ 5 ℃.

이하에서는 탁주용 정체효소, 밀가루 누룩, 효모액을 사용하지 않은 원주와 본 발명에 따라 제조된 개량주의 제조 방법에 따른 전통주의 발효 특성 및 관능평가 결과를 비교한다.
Hereinafter, the fermentation characteristics and sensory evaluation results of traditional liquors according to the production method of the modified liquor prepared according to the present invention and the circumference without using the turmeric enzyme, wheat flour yeast and yeast solution are compared.

(비교예 - 원주)(Comparative Example-Wonju)

분쇄한 찹쌀 400g과 용수 800mL 첨가하여 죽을 제조한 후 냉각하여 밀가루 누룩 400g을 첨가하고 25℃에서 3일간 발효시켜 5℃에 보관하면서 주모로 사용한다. 상기와 같이 제조된 주모 1L, 증자한 찹쌀(중량 2kg), 담금 용수 2.6L, 찹쌀 100중량부에 밀가루 누룩 10중량부를 첨가하고 25℃에서 12시간 간격으로 교반하여 3일간 발효시킴으로써 1단 담금한다. 1단 담금된 술덧에 증자한 찹쌀(중량 8 kg), 담금 용수 12L를 첨가하고 25℃에서 12일(288시간)간 발효시켜 2단 담금하여 제조한다.
Add 400g of crushed glutinous rice and 800mL of water to make porridge, and then cool it, add 400g of flour nuruk, ferment at 25 ℃ for 3 days, and use it as the main hair while keeping it at 5 ℃. 1L of jujuma prepared as described above, steamed glutinous rice (weight 2kg), immersion water 2.6L, 100 parts of glutinous rice is added 10 parts by weight of wheat flour koji and stirred at 25 ° C. for 12 hours to ferment for 3 days. . Prepared by adding 1L of glutinous rice (8 kg in weight) and dipping water 12L and adding fermentation at 25 ℃ for 12 days (288 hours).

(실시예 - 개량주)(Example-modified liquor)

분쇄한 찹쌀 400g, 용수 800mL와 탁주용 정제효소를 찹쌀 100중량부에 대해 0.15중량부 첨가하여 죽을 제조한 후 냉각하여 밀가루 누룩 400g, 배양시킨 효모액 (Saccharomyces cerevisiae KCCM 32021) 50mL, 주모 총량 100중량%당 0.5중량% 포함되도록 젖산을 첨가하고 25℃에서 3일(72시간)간 발효시켜 5℃에 보관하면서 주모로 사용한다. 상기와 같이 제조된 주모 1L당, 증자한 찹쌀 2kg, 담금 용수 2.6L, 찹쌀 100중량부 기준으로 10중량부의 밀가루 누룩, 0.15중량부의 탁주용 정제효소를 첨가하고 25℃에서 12시간 간격으로 교반하여 3일간 발효시킴으로써 1단 담금한다. 1단 담금된 술덧에 증자한 찹쌀 8kg, 담금 용수 12L 및 찹쌀 100중량부에 0.3중량부인 탁주용 정제효소를 첨가하고 25℃에서 12일(288시간)간 발효시켜 2단 담금하여 제조한다.
400 g of ground glutinous rice, 800 mL of water and 0.15 parts by weight of Takju purified enzyme were added to 100 parts by weight of glutinous rice to make porridge, and then cooled and 400 g of wheat yeast, cultured yeast solution (Saccharomyces cerevisiae KCCM 32021), 50 mL Lactic acid is added to contain 0.5% by weight, fermented at 25 ° C. for 3 days (72 hours), and stored at 5 ° C. to be used as main hair. Per 1L of the prepared jeju, 2kg of cooked glutinous rice, dipping water 2.6L, 100 parts by weight of glutinous rice based on 10 parts by weight of wheat yeast, 0.15 parts by weight of purified tablet enzyme was added and stirred at 25 ° C for 12 hours It is immersed in 1 stage by fermentation for 3 days. 8 kg of glutinous rice, steamed water 12L, and 100 parts of glutinous rice, 0.3 parts by weight of Takju purified enzyme were added to the first step soaked rice wine, and fermented at 25 ° C. for 12 days (288 hours).

(발효 특성 비교)(Fermentation characteristics comparison)

- pH pH

전통주의 발효기간 중 pH 변화는 그래프 1에서 보는 바와 같다. 발효 2일에는 pH가 4.3 ~ 4.4이고 발효 6일에는 모든 시료구의 pH가 감소하여 3.6 ~ 3.7로 모든 시료구가 완만히 감소하는 경향을 보이고, 8일 이후 큰 변화를 보이지 않는다. The pH change during the fermentation of traditional liquor is shown in Graph 1. On the second day of fermentation, the pH was 4.3 to 4.4 and on the sixth day of fermentation, the pH of all the sample spheres decreased to 3.6 to 3.7, showing a tendency to slowly decrease all the sample spheres.

<그래프 1. 발효기간 중 pH 변화><Graph 1. pH change during fermentation period>

Figure 112011043721126-pat00001
Figure 112011043721126-pat00001

A : 원주, B : 개량주
A: Wonju, B: Improved

- 산도-Acidity

전통주의 총산 변화는 그래프 2에서 보는 바와 같다. 담금 2일에는 총산 함량이 0.45 ~ 0.47%이었으며 발효 6일에는 급격히 증가하여 0.86 ~ 0.87%로 나타난다. 발효 10일 이후 총산 함량은 큰 변화를 보이지 않으며 시료구별 유의적 차이는 없다. The total change in traditionalism is shown in Graph 2. The total acid content was 0.45 ~ 0.47% on the 2nd day of immersion and rapidly increased to 0.86 ~ 0.87% on the 6th day of fermentation. After 10 days of fermentation, total acid content did not change significantly and there was no significant difference between samples.

<그래프 2. 발효기간 중 총산 변화><Graph 2. Total Acid Changes during Fermentation>

Figure 112011043721126-pat00002
Figure 112011043721126-pat00002

A : 원주, B : 개량주
A: Wonju, B: Improved

- 환원당 함량-Reducing sugar content

전통주의 환원당 함량 변화는 그래프 3에서 보는 바와 같다. 발효 2일에는 14.92~ 15.06%이나 발효가 왕성하게 진행된 6일까지 큰 폭으로 감소하여 6.26 ~ 6.54%로 나타난다. 발효 8일 경과 후 소폭으로 감소하여 발효가 끝나는 12일에는 4.70 ~ 4.94%로 나타난다. Changes in reducing sugar content in traditional stocks are shown in Graph 3. On the 2nd day of fermentation, it is 14.92 ~ 15.06%, but it decreases sharply until the 6th day of fermentation, which is 6.26 ~ 6.54%. After 8 days of fermentation, it is slightly decreased, and it is 4.70 ~ 4.94% at 12 days after fermentation.

<그래프 3. 발효기간 중 환원당 함량 변화><Graph 3. Reducing Sugar Contents during Fermentation>

Figure 112011043721126-pat00003
Figure 112011043721126-pat00003

A : 원주, B : 개량주
A: Wonju, B: Improved

- 알코올 함량Alcohol content

전통주의 알코올 함량 변화는 그래프 4에서 보는 바와 같다. 발효 2일에는 6.84 ~ 7.04%이며 6일까지 급격히 증가하여 14.26 ~ 14.53%로 나타난다. 발효 8일 이후에는 증가폭이 둔화되어 발효가 끝나는 12일에 최종 알코올 함량은 15.44 ~ 15.71%로 나타나며, 이러한 알코올 농도의 증가 현상은 환원당 함량의 감소 경향과 일치한다.Traditional alcohol content changes are shown in graph 4. On the 2nd day of fermentation, it is 6.84 ~ 7.04% and it increases rapidly until 6 days and shows 14.26 ~ 14.53%. After 8 days of fermentation, the increase slowed down, and the final alcohol content was found to be 15.44 ~ 15.71% at 12 days after fermentation. The increase in alcohol concentration is consistent with the decreasing tendency of reducing sugar content.

<그래프 4. 발효기간 중 알코올 함량 변화><Graph 4. Alcohol Content Changes During Fermentation>

Figure 112011043721126-pat00004
Figure 112011043721126-pat00004

A : 원주, B : 개량주
A: Wonju, B: Improved

- 유리당 함량Free sugar content

전통주의 유리당 함량은 표 3에서 보는 바와 같다. 전통주에서 확인된 유리당은 포도당, 맥아당으로 나타난다. 원주의 포도당과 맥아당 함량은 각각 387.51 mg%, 36.43 mg%로 나타나고, 개량주의 포도당과 맥아당 함량은 401.21 mg%, 24.73 mg%로 나타나며, 총 유리당 함량은 각각 423.94 mg%, 425.94 mg%로 유사한 경향을 보인다.
The free sugar content of traditional wines is shown in Table 3. Free sugars found in traditional liquor appear as glucose and maltose. The glucose and maltose contents of the columnar were 387.51 mg% and 36.43 mg%, respectively, and the glucose and maltose content of modified strains were 401.21 mg% and 24.73 mg%, respectively. The total free sugar content was 423.94 mg% and 425.94 mg%, respectively. see.

구분division 원주Won-ju 개량주Refinement 포도당glucose 387.51387.51 401.21401.21 맥아당Maltose 36.4336.43 24.7324.73 총 당Per total 423.94423.94 425.94425.94

(mg%)
(mg%)

- 유기산 함량-Organic acid content

전통주 유기산 분석 결과는 표 4에서 보는 바와 같이 젖산만 검출 되었으며 함량은 원주가 132.42 mg%, 개량주가 147.65 mg%로 나타나 개량주가 11.5% 증가된 것을 확인할 수 있다.
As shown in Table 4, only the lactic acid was detected, and the content of the traditional liquor was 132.42 mg% and the modified liquor was 147.65 mg%, indicating that the modified liquor increased by 11.5%.

유기산Organic acid 원주Won-ju 개량주Refinement 젖산Lactic acid 132.42132.42 147.65147.65

(mg%)
(mg%)

- 향기성분 함량-Aroma content

전통주의 향기성분 분석 결과는 표 5에서 보는 바와 같이 9종의 향기성분이 검출 되었으며, 주요 향기 성분으로는 iso-amylalcohol, 1, 3-propanediol, iso-Butyl alcohol 등으로 주로 알코올 성분이 주종을 이룬다.
As a result of traditional fragrance component analysis, 9 kinds of fragrance components were detected as shown in Table 5. The main fragrance components are mainly alcohol components such as iso-amylalcohol, 1, 3-propanediol and iso-Butyl alcohol. .

구분division 원주Won-ju 개량주Refinement Acetic acidAcetic acid 6.846.84 8.228.22 Ethyl esterEthyl ester 3.263.26 7.737.73 iso-Butyl alcoholiso-Butyl alcohol 11.0811.08 6.856.85 1, 3-Propanediol1, 3-Propanediol 21.6721.67 17.9417.94 iso-amylalcoholiso-amylalcohol 47.0247.02 53.3753.37 Butanoic acidButanoic acid 3.253.25 0.730.73 1-Butanol1-Butanol 4.314.31 2.362.36 2-Methyl alcohol2-Methyl alcohol 2.182.18 2.712.71 불명Unknown 0.390.39 0.090.09

(area%)
(area%)

(전통주 관능평가)( Sensory Evaluation of Traditional Wine )

전통주의 관능평가 결과는 표 6과 같다. 향과 맛에서는 개량주가 각각 4.0, 4.1로 원주보다 좋은 기호도를 보였고, 색은 차이를 보이지 않는다. 종합적인 기호도를 살펴보면 개량주가 4.1로 원주 3.9보다 높은 점수를 받고 있다.
The results of traditionalism sensory evaluation are shown in Table 6. In terms of aroma and taste, modified liquors were 4.0 and 4.1, respectively. Looking at the overall acceptability, improved stock prices are 4.1, higher than Won 3.9.

구분
division
기호도Likelihood
incense color flavor 종합 기호도Comprehensive preference map 원주Won-ju 3.83.8 3.93.9 3.93.9 3.93.9 개량주Refinement 4.04.0 3.93.9 4.14.1 4.14.1

전통주는 청징 및 냉장보관 단계 이후에 병입하여 중탕으로 살균처리하는데, 전통주의 처리 온도 및 시간에 따른 미생물의 살균 효과를 실험한 결과는 표 7, 8에서 보는 바와 같다. 표 7, 8에서 대조구인 열처리 살균 하지 않은 생주의 세균은 7.2×05 CFU/, 젖산균은 1.3×106 CFU/, 효모는 8.1×105CFU/로 나타난다. Traditional liquor is bottled after clarification and refrigeration and sterilized in a bath. The results of experiments on the sterilization effect of microorganisms according to the treatment temperature and time of traditional liquor are shown in Tables 7 and 8. In Tables 7 and 8, the bacteria of the untreated heat-treated bacterium were 7.2 × 0 5 CFU /, the lactic acid bacteria were 1.3 × 10 6 CFU /, and the yeast was 8.1 × 10 5 CFU /.

구체적으로 전통주의 처리 온도 및 시간에 따른 미생물의 살균 효과를 실험한 결과 처리온도별 미생물의 살균효과를 살펴보면 시료구마다 약간의 차이는 있으나, 대체로 세균은 80℃에서, 젖산균과 효모는 60℃에서 살균효과가 있다. 또한 미생물의 살균효과를 시간대별로 살펴보면 세균은 70℃에서 30분, 80℃에서 10분 이후로 살균 효과가 나타나고, 젖산균은 60℃℃에서 10분, 효모는 60에서 15분 이후로 살균효과가 나타난다. 따라서 종합적으로 처리온도 및 시간에 따른 미생물의 살균 효과는 세균, 젖산균 및 효모의 살균 효과가 모두 나타난 80℃에서 10분간 열처리 하는 방법이 가장 바람직하다.
Specifically, as a result of experiments on the sterilization effect of microorganisms according to the treatment temperature and time of traditional liquor, the microbial sterilization effect according to the treatment temperature was slightly different for each sample, but the bacteria were generally at 80 ℃ and the lactic acid bacteria and yeast at 60 ℃. It has a bactericidal effect. In addition, when looking at the sterilization effect of the microorganism by time, the bactericidal effect is shown after 30 minutes at 70 ℃, 10 minutes at 80 ℃, lactic acid bacteria after 10 minutes at 60 ℃ ℃, yeast 60 to 15 minutes after . Therefore, the overall microbial sterilization effect according to the treatment temperature and time is the most preferable method of heat treatment for 10 minutes at 80 ℃ showing the bactericidal effect of bacteria, lactic acid bacteria and yeast.


미생물

microbe

대조구
(무처리)

Control
(No treatment)
가열온도(℃), 시간(분)Heating temperature (℃), time (minutes)
60℃60 ° C 70℃70 ℃ 5분5 minutes 10분10 minutes 15분15 minutes 20분20 minutes 30분30 minutes 5분5 minutes 10분10 minutes 15분15 minutes 세균Germ 7.2×105 7.2 × 10 5 6.5×105 6.5 × 10 5 3.2×105 3.2 × 10 5 7.3×104 7.3 × 10 4 1.5×103 1.5 × 10 3 4.2×101 4.2 × 10 1 6.8×103 6.8 × 10 3 1.1×103 1.1 x 10 3 2.2×102 2.2 × 10 2 젖산균Lactic acid bacteria 1.3×106 1.3 × 10 6 5.7×102 5.7 × 10 2 2.8×101 2.8 × 10 1 -- -- -- -- -- -- 효모leaven 8.1×105 8.1 × 10 5 3.1×103 3.1 x 10 3 1.9×101 1.9 × 10 1 -- -- -- -- -- --


미생물

microbe
가열온도(℃), 시간(분)Heating temperature (℃), time (minutes)
70℃70 ℃ 80℃80 ℃ 90℃90 ° C 100℃100 ℃ 20분20 minutes 30분30 minutes 5분5 minutes 10분10 minutes 15분15 minutes 20분20 minutes 30분30 minutes 5분5 minutes 10분10 minutes 15분15 minutes 20분20 minutes 30분30 minutes 5분5 minutes 10분10 minutes 20분20 minutes 세균Germ 1.8×101 1.8 × 10 1 -- 2.1×101 2.1 × 10 1 -- -- -- -- -- -- -- -- -- -- -- -- 젖산균Lactic acid bacteria -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 효모leaven -- -- -- -- -- -- -- -- -- -- -- -- -- -- --

한편, 전통주의 살균 처리온도 및 시간에 따른 색도 측정 결과 온도별로 보면 명도는 60 ~ 100℃까지 온도가 증가 할수록 감소하는 경향을 보이고, 적색도와 황색도는 온도가 증가 할수록 소폭 증가하는 경향을 보인다. 처리 온도 및 시간을 달리하여 살균한 전통주의 관능평가 결과 처리 온도별 관능평가 결과를 보면 색은 온도증가와 관련해 별 차이가 없었고, 맛과 냄새는 80℃까지는 크게 저하되지 않았으나, 90 ~ 100℃ 까지 열처리한 전통주는 생주보다 맛과 냄새가 유의적으로 떨어져 쓴맛과 화독 냄새가 강하게 나타난다.
On the other hand, as a result of chromaticity measurement according to the sterilization treatment temperature and time of traditional wine, the brightness tends to decrease as the temperature increases to 60 ~ 100 ℃, and the redness and yellowness tend to increase slightly as the temperature increases. Sensory Evaluation Results of Traditional Wines Sterilized with Different Treatment Temperatures and Times The results of sensory evaluation by treatment temperature showed no significant differences in the temperature increase, and taste and odor did not drop significantly up to 80 ℃, but up to 90 ~ 100 ℃. Heat-treated traditional liquor is significantly lower in taste and smell than raw liquor, resulting in a stronger bitter and poisonous smell.

Claims (10)

찹쌀을 씻어 4~6시간 물에 침지한 후에 4~6시간 탈수하여 준비하는 원료 세척 및 침지 단계;
준비된 찹쌀을 분쇄하고, 물과 탁주용 정제효소를 첨가하여 죽을 제조한 후 실온으로 냉각하는 죽 제조 단계;
냉각시킨 죽에 밀가루 누룩, 효모액, 젖산을 첨가하고, 72시간 동안 23℃로 발효시켜 주모를 제조한 후 5℃로 냉각하는 주모 제조 단계;
제조된 주모 1L당, 증자한 찹쌀 2kg을 고두밥으로 제조한 것, 물 2.6L, 찹쌀 100중량부 기준으로 9~11중량부의 밀가루 누룩, 0.13~0.17중량부의 탁주용 정제효소를 첨가하고, 25℃에서 12시간 간격으로 교반하여 3일(72시간)간 발효시키는 1단 담금 단계;
1단 담금된 술덧 5.8kg당, 증자한 찹쌀 8kg을 고두밥으로 제조한 것, 물 12L, 찹쌀 100중량부의 0.2~0.4중량부인 탁주용 정제효소를 첨가하고 25℃에서 12일(288시간)간 발효시키는 2단 담금 단계;
를 포함하는 전통 발효주 제조 방법.
Washing and dipping the glutinous rice and then immersing in water for 4-6 hours and then dehydrating for 4-6 hours;
Grind the prepared glutinous rice, and add the water and Takju purified enzyme to prepare the porridge and then the porridge production step of cooling to room temperature;
Flour nuruk, yeast solution, lactic acid is added to the cooled porridge, fermented at 23 ℃ for 72 hours to prepare a cast hair and then cooled to 5 ℃;
Per 1L of the prepared jujube, 2kg of cooked glutinous rice was prepared with gourd rice, 2.6L of water and 9-11 parts by weight of wheat flour malt, 0.13-0.17 parts by weight of purified tablet enzyme, based on 100 parts by weight of glutinous rice, and 25 ° C. 1 step immersion step of fermentation for 3 days (72 hours) by stirring at intervals of 12 hours;
5.8kg per 1 soaked rice wine, 8kg of cooked glutinous rice made with gourd rice, 12L of water, 100 parts of glutinous rice, 0.2 ~ 0.4 parts by weight of Takju purified enzyme and fermented at 25 ℃ for 12 days (288 hours) Two stage immersion step to make;
Traditional fermented liquor manufacturing method comprising a.
제1항에 있어서,
상기 죽 제조 단계는
분쇄된 찹쌀 400g당, 물 800mL, 찹쌀 100중량부 기준으로 0.13~0.17중량부인 탁주용 정제효소를 첨가하여 제조하는 것을 특징으로 하는 전통 발효주 제조 방법.
The method of claim 1,
The porridge manufacturing step
400 g of ground glutinous rice, 800mL of water, 0.13 ~ 0.17 parts by weight based on 100 parts by weight of glutinous rice, the traditional fermented liquor manufacturing method characterized in that the production by adding the purified enzyme.
제1항에 있어서,
상기 주모 제조 단계는
제조된 죽 1.2kg 기준으로, 밀가루 누룩 400g, 효모액 50mL, 주모 100중량%에 0.3~0.7중량% 포함되는 젖산을 첨가하여 제조하는 것을 특징으로 하는 전통 발효주 제조 방법.
The method of claim 1,
The main hair manufacturing step
Based on the 1.2kg of the prepared porridge, 400g wheat flour yeast, 50mL yeast solution, 100% by weight of lactic acid containing 0.3 ~ 0.7% by weight is added to prepare a traditional fermented liquor manufacturing method.
삭제delete 삭제delete 제1항에 있어서,
상기 주모 제조 단계에서 첨가되는 상기 효모액은 Saccharomyces cerevisiae KCCM 32021인 것을 특징으로 하는 전통 발효주 제조 방법.
The method of claim 1,
The yeast liquor added in the step of preparing the main fermentation method of Saccharomyces cerevisiae KCCM 32021 characterized in that the production method.
제1항에 있어서,
상기 주모 제조 단계에서 첨가되는 상기 밀가루 누룩은 밀가루 100중량부에 30중량부의 정제수를 첨가하여 110℃에서 15분간 고압증기로 살균하고,
종국(seed koji)으로 제조된 균주를 성형없이 30℃에서 24시간 1차 배양하며,
1차 배양한 밀가루 누룩을 성형한 후 30℃, 습도 85%에서 48시간 2차 배양하고,
30℃에서 수분함량이 9~11%가 되게 건조하여 제조하는 것을 특징으로 하는 전통 발효주 제조방법.
The method of claim 1,
The wheat flour yeast added in the step of preparing the main hair is sterilized by autoclaving at 110 ° C. for 15 minutes by adding 30 parts by weight of purified water to 100 parts by weight of wheat flour,
Strains prepared as seed koji were firstly incubated at 30 ° C. for 24 hours without molding,
After molding the first cultured wheat flour yeast second culture for 48 hours at 30 ℃, 85% humidity,
Traditional fermented liquor manufacturing method characterized in that the dried to produce a water content of 9 ~ 11% at 30 ℃.
제1항에 있어서,
2단 담금한 술덧을 3000rpm으로 30분간 원심분리하여 청징한 후, 1~5℃로 냉장 보관하는 청징 및 냉장 단계를 더 포함하는 것을 특징으로 하는 전통 발효주 제조방법.
The method of claim 1,
After centrifuging and quenching the two-stage dipped soot at 3000 rpm for 30 minutes, the traditional fermented liquor manufacturing method further comprises the clarification and refrigeration step of refrigeration at 1 ~ 5 ℃.
제1항에 있어서,
병입하여 중탕으로 80℃에서 10분간 열처리하는 살균 단계를 더 포함하는 것을 특징으로 하는 전통 발효주 제조방법.
The method of claim 1,
Traditionally fermented liquor manufacturing method further comprises a sterilization step of heat-treating 10 minutes at 80 ℃ in a heated bath.
제1항에 있어서,
상기 1단 담금 단계, 2단 담금 단계에서,
상기 고두밥은 100중량% 기준으로 찹쌀이 70~75중량%, 수분이 25~30중량%포함되는 것을 특징으로 하는 전통 발효주 제조방법.
The method of claim 1,
In the first stage immersion step, the second stage immersion step,
The gourd rice is a traditional fermented liquor manufacturing method, characterized in that the glutinous rice 70 ~ 75% by weight, 25 ~ 30% by weight based on 100% by weight.
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