KR101250198B1 - Composition of food components for appetite control comprising specific compounds as effective component - Google Patents

Abstract

The present invention is ascorbic acid (ascorbic acid), beta-D-glucan (β-D-glucan), hesperetin (hesperetin), melatonin (melatonin), ginsenoside-Rb1 (ginsenoside-Rb1) and gin The present invention relates to an appetite suppressant composition containing at least one component selected from the group consisting of ginsenoside-Rg1, and more particularly, has an appetite suppressing effect by inhibiting the activity of ghrelin, an appetite-stimulating hormone. Appetite suppressant compositions and ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin, melatonin, ginsenoside-Rb1 and ginsenosides -Rg1 (ginsenoside-Rg1) relates to a functional food for suppressing appetite containing at least one component selected from the group consisting of.

Description

Composition of food components for appetite control comprising specific compounds as effective component

The present invention as an active ingredient (ascorbic acid), beta-D-glucan (β-D-glucan), hesperetin (hesperetin), melatonin (melatonin), ginsenoside-Rb1 (ginsenoside-Rb1) and gin Appetite suppressant composition and ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin containing at least one component selected from the group consisting of ginsenoside-Rg1. ), Melatonin, melatonin, ginsenoside-Rb1 (ginsenoside-Rb1) and ginsenoside-Rg1 (ginsenoside-Rg1) relates to a functional food for suppressing appetite containing an active ingredient containing at least one component will be.

Obesity is the highest in the United States (32% of the population), with a body mass index (BMI) of 30 or higher, and Mexico, 30%, the UK, 23%, Greece, Australia, and New Zealand, 21%. have. In Korea, 1.5% to 2 million people, 3.5% of the total population, are obese, but the population of obesity is increasing very rapidly due to westernization and diversification of dietary life.

Obesity has led to adult disease, including diabetes, hypertension, cardiovascular disease, cancer and mental distress, to some of the causes of death, and also affects national productivity, activity and competitiveness. In particular, the obesity of children and adolescents in Korea, which has undergone many changes in their diet and educational environment, is increasing rapidly, and the recovery of treatment after the onset of pediatric diabetes, etc., is not easy.

Obesity-related treatments have been tried in various ways such as drug administration, exercise, diet, psychotherapy, and surgery, but it is difficult to improve by specific methods, and complex treatments and patient will are important. In the case of drug administration, the drug was developed on the basis of lipolysis or digestive enzyme inhibition, but it has side effects.

As a solution to obesity, a method of degrading and accumulating mast cells or inhibiting digestive enzymes has been generally attempted. If food is supplied to the body, digestion or lipolysis has a limited role compared to the rate and amount of ingestion.

Thus, appetite control (appetite control) to fundamentally regulate the intake of food can be an approach to the fundamental solution, the appetite suppression by the food ingredient composition as a first attempt is meaningful as an invention with novelty, originality and progressiveness Have

Currently, reductil has been released as a drug to control obesity due to appetite control, but it is required to access food or natural products with side effects such as loss of appetite, insomnia, depression, and blood pressure. Therefore, there is a need for a composition for appetite suppression that can contribute to suppression of obesity by creating a new value as an appetite suppressing food material while minimizing side effects.

The present invention first developed an appetite suppression method by a food ingredient composition as a means of preventing obesity, and in the method of proving appetite suppression effect, first discovered a test system that suppresses ghrelin that promotes appetite, and thus on the degree of cell differentiation. Therefore, food ingredients were applied and appetite suppression effects were scientifically verified.

Korean Patent Publication No. 2004-0097813 discloses an anti-obesity functional composition having three functions such as appetite suppression, body fat reduction, and bowel activation. Korean Patent Publication No. 2004-0036111 discloses ginseng and polyphenol-based substances or bioflavonoids. Foods for improving lipid metabolism and anti-obesity including a powder or extract of a plant comprising a systemic substance have been disclosed, but differ from the appetite suppressing food ingredient composition of the present invention.

The present invention is derived from the above requirements, the present invention is desired to develop a functional material that can suppress the appetite, but many limitations due to safety problems such as limitations and side effects of body fat decomposition, digestion inhibition method Nowadays, ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin, melatonin, melaninin, ginsenoside-Rb1 (which is a food ingredient and can suppress appetite) The present invention was completed by developing a composition for suppressing appetite containing at least one component selected from the group consisting of ginsenoside-Rb1) and ginsenoside-Rg1 (ginsenoside-Rg1) as an active ingredient.

In addition, the present invention first developed an appetite suppression method by a food ingredient composition as a solution to obesity suppression, and also in the method of demonstrating the appetite suppression effect, by first discovering a test system that suppresses ghrelin that promotes appetite, cells According to the degree of differentiation, food ingredients were applied and appetite suppression effect was scientifically verified.

In order to solve the above problems, the present invention as an active ingredient ascorbic acid (ascorbic acid), beta-D-glucan (β-D-glucan), hesperetin (hesperetin), melatonin (melatonin), ginsenoside-Rb1 It provides a composition for suppressing appetite containing at least one component selected from the group consisting of (ginsenoside-Rb1) and ginsenoside-Rg1 (ginsenoside-Rg1).

In addition, the present invention is ascorbic acid (ascorbic acid), beta-D-glucan (β-D-glucan), hesperetin (hesperetin), melatonin (melatonin), ginsenoside-Rb1 (ginsenoside-Rb1) and ginsenosides It provides a functional food for suppressing appetite containing at least one component selected from the group consisting of side-Rg1 (ginsenoside-Rg1) as an active ingredient. In the method of proving appetite suppression effect, a test system that suppresses ghrelin that promotes appetite was first discovered, and food ingredients were applied according to the degree of cell differentiation, and the appetite suppression effect of food ingredients was scientifically verified.

According to the present invention, the appetite suppressant composition of the present invention is effective for suppressing appetite, and because it uses food ingredients, no side effects appear even after taking for a long time, and it is possible to secure safety, very useful in functional food industry for appetite suppression It will be an invention.

In addition, the method to prove the appetite suppression effect was the first to discover the ghrelin test system that promotes appetite, and to provide the first model to scientifically verify the appetite suppression effect by applying food ingredients according to the degree of cell differentiation.

1 shows the concentration of ghrelin as a multinucleate differentiation inhibitory activity when 1 or 10 ㎍ / ml of each food ingredient is added to human umbilical vein endothelial cells (HUVEC) cells. .
1: sibutramine (positive control), 2: ascorbic acid, 3: beta-D-glucan, 4: hesperetin, 5: alpha-lipoic acid (α) -lipoic acid), 6: serotonin, 7: melatonin, 8: alpha-tocopherol, 9: ginsenoside-Rb1, 10: ginsenoside-Rg1 (ginsenoside-Rg1), 11: naringenin
FIG. 2 shows that each food ingredient is treated at 60 ° C., 80 ° C., and 100 ° C. for 5 minutes, and then food ingredients are added to human umbilical vein endothelial cells (HUVECs), respectively, to determine cell proliferation and ghrelin levels. It is measured.
1: sibutramine (positive control), 2: ascorbic acid, 3: beta-D-glucan, 4: hesperetin, 5: alpha-lipoic acid (α) -lipoic acid), 6: serotonin, 7: melatonin, 8: alpha-tocopherol, 9: ginsenoside-Rb1, 10: ginsenoside-Rg1 (ginsenoside-Rg1), 11: naringenin
FIG. 3 shows that each food ingredient is treated within 5 minutes at pH 2, 5, and 7, and then the food ingredient is added to human umbilical vein endothelial cells (HUVEC) cells, respectively, to measure cell proliferation and ghrelin. It is.
1: sibutramine (positive control), 2: ascorbic acid, 3: beta-D-glucan, 4: hesperetin, 5: alpha-lipoic acid (α) -lipoic acid), 6: serotonin, 7: melatonin, 8: alpha-tocopherol, 9: ginsenoside-Rb1, 10: ginsenoside-Rg1 (ginsenoside-Rg1), 11: naringenin

In order to achieve the object of the present invention, the present invention is ascorbic acid (ascorbic acid), beta-D-glucan (β-D-glucan), hesperetin (hesperetin), melatonin (melatonin), ginsenoside Provided is a composition for suppressing appetite containing at least one component selected from the group consisting of -gin (R) (ginsenoside-Rbl) and ginsenoside (Rg1).

In the composition of the present invention, the appetite suppression may be an effect of inhibiting the activity of ghrelin (ghrelin), an appetite-stimulating hormone.

The active ingredient included in the composition of the present invention may maintain ghrelin inhibitory activity even after 5 minutes treatment at a temperature of 60 ~ 100 ℃. From this, the ghrelin inhibitory activity is maintained even when the food ingredient is heated, thereby indirectly confirming the possibility of maintaining appetite suppressing activity of the food ingredient during processing.

In addition, the active ingredient included in the composition of the present invention can maintain the ghrelin inhibitory activity even after 5 minutes treatment at pH 2 ~ 10. From this, it was indirectly determined that the appetite suppression effect was maintained overall by suppressing ghrelin activity even when ingesting food components in the body.

Appetite suppressing compositions of the present invention may include suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical dosage forms of the appetite suppressant composition of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds, as well as in a suitable collection.

The appetite suppressing composition according to the present invention is formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Can be used. Carriers, excipients and diluents that may be included in the appetite suppressant composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Various compounds or mixtures, including cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the appetite suppressant composition of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the appetite suppressing composition of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The appetite suppressant composition of the present invention can be administered to various mammals such as mice, mice, livestock, humans. All modes of administration can be expected, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The present invention also provides ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin, melatonin, ginsenoside-Rb1 and ginsenoside. It provides a functional food for suppressing appetite containing at least one component selected from the group consisting of side-Rg1 (ginsenoside-Rg1) as an active ingredient.

When the appetite suppressing composition of the present invention is used as a food additive, the appetite suppressing composition may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, in the manufacture of food or beverages the components of the invention are added in an amount of up to 15 parts by weight, preferably up to 10 parts by weight relative to the raw materials. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all foods in a conventional sense.

The functional beverage composition containing the meat suppressing composition of the present invention may contain various flavors or natural carbohydrates as additional components, as in general beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the appetite suppressing composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the appetite suppressing composition of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

1. Securing Sample

(1) Selection of appetite suppression material

The food ingredient for appetite suppression test system was to be a single ingredient, and ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin, Lipoic acid, serotonin, serotonin, melatonin, tocopherol, ginsenoside-Rb1, ginsenoside-Rg1, ginsenoside-Rg1, naringenin, sibutramine (sibutramin, positive control) was secured.

(2) Preparation of Cell Test Sample Solution

The target food ingredients were dissolved in DMSO or distilled water, diluted and used for each concentration. The final concentration of DMSO was 0.1% or less to prepare a cell test sample.

2. Appetite Promoting Hormones Ghrelin  Active test

(1) HUVEC cell culture

HUVEC (human umbilical vein endothelial cell) was used by Young Science. 37 ° C using EGM-2 medium (FBS, Hydrocortisone, hFGF, VEGF.R3-IGF-1, Ascorbic acid, hEGF, GA-1000, Heparin) in a 100 mm dish coated with 2% gelatin , And incubated in an incubator maintained at 5% CO ₂ . When the cells were confluent, the cells were passaged by treatment with trypsin-EDTA, and the medium was exchanged every 2 to 3 days.

(2) ghrelin assay

Samples for ghrelin analysis were separated into supernatant and cell lysate. After the end of the experiment, the recovered culture was centrifuged (3,000 rpm, 20 minutes, 4 ℃) to recover only the supernatant, cell lysate was centrifuged for 10 minutes at 10000 rpm after separating the cells with Trypsin-EDTA. After removing the supernatant and preparing a cell lysate using a solution containing 1% of Triton X-100 in the obtained cells, the content of ghrelin was measured using a HUMAN unacylated ghrelin kit (SPI-Bio). That is, in the measurement of ghrelin, 100 ul of standard diluent buffer and 100 ul of sample were put in a well, and 100 ul of anti-unacylated ghrelin-AChE tracer was added. After 3 hours of incubation at room temperature, the culture solution was completely removed and washed 5 times with diluted wash solution. After drying the plate, absorbance was measured at 405 nm after adding 200 ul of Ellman's reagent.

3. Temperature and pH Of food ingredients Cell proliferation Ghrelin ( ghrelin ) Inhibitory activity

(1) Changes in Cell Proliferation and Ghrelin Inhibition Activation of Food Ingredients with Temperature

In order to investigate the stability during processing of food ingredients, the inhibitory activity of ghrelin, an appetite-stimulating hormone according to heating temperature, was investigated for food ingredient concentrations that had high inhibitory activity against ghrelin. After a certain amount of food ingredients were treated at 60 ° C., 80 ° C. and 100 ° C. for 5 minutes, and prepared at concentrations having high inhibitory activity against ghrelin, the cell proliferation ability and the amount of ghrelin were measured.

(2) Changes in Cell Proliferation and Ghrelin Inhibition Activation of Food Ingredients According to pH

To indirectly test the body's activity of appetite suppression of food ingredients, the ghrelin inhibitory activity was investigated according to the pH change of food ingredients under acidic, neutral and basic conditions. Each food solution was dipped in each pH solution at a concentration of 10% within 5 minutes, and then lyophilized to prepare a concentration having high inhibitory activity against ghrelin, and then the cell proliferation ability and the amount of ghrelin were measured.

Example  1: When adding food ingredients Ghrelin  Concentration change

When food ingredients were added to human umbilical vein endothelial cells (HUVEC) cells at 1 or 10 μg / ml, respectively, the concentration of ghrelin was measured as multinucleate differentiation inhibitory activity compared to the control (FIG. 1). In the multinucleate state, the samples were treated by setting the time of rapid increase in the proliferation rate to 30 hours of passage culture.

As a result, sibutramine, a positive control, had a content of 75% (25% inhibition) at a concentration of 1 ug / ml, and high ghrelin (68% (32% inhibition) at 10 ug / ml). Inhibitory activity was ascorbic acid (ascorbic acid) showed a secretion rate of 93% and 94% at concentrations of 1 ug / ml and 10 ug / ml, respectively. In the case of glucan, the concentration of ghrelin decreased as the concentration of sample increased in concentration-dependent manner, showing the lowest content of 85% at the concentration of 10 ug / ml, and 1 ug / for hesperetin. At the concentration of ml, the highest inhibitory activity was shown to be 79%, and low value of 85% even at 10 ug / ml. Melatonin had a low ghrelin content of 87% at 10 ug / ml, and ginsenoside-Rb1 gradually decreased with increasing concentrations to 76% at 10 ug / ml. Ginsenoside-Rg1 (ginsenoside-Rg1) also showed a tendency to decrease the content of ghrelin in a concentration-dependent manner, showing a low content of 84% at 10 ug / ml.

Thus, ascorbic acid, beta-D-glucan, β-D-glucan, hesperetin, melatonin, ginsenoside-Rb1 and ginsenoside-Rg1 Compared to the control in the (ginsenoside-Rg1) treatment, the content of ghrelin was decreased, and it was confirmed that the appetite suppression effect was observed.

Example  2: of food ingredients according to temperature Cell proliferation Ghrelin ( ghrelin Inhibitory activation changes

The proliferative capacity of the cells (HUVEC) and the content of ghrelin according to the heating temperature of the food ingredient are shown in FIG. 2. The cell proliferative capacity according to temperature was found to be maintained overall even when the temperature was increased to 60 ~ 100 ℃. In addition, it was found that the content of ghrelin according to the temperature change is maintained stably. Ghrelin (ghrelin) is mostly 80 ~ 90% content in the temperature range of 60 ~ 100 ℃ it was judged that the inhibitory effect is maintained about 20 to 10%. From this, even when the food ingredients are heated, cell proliferation and ghrelin inhibitory activity is maintained, thereby indirectly confirming the possibility of maintaining appetite suppressing activity of the food ingredients during processing.

Example  3: pH Of food ingredients Cell proliferation Ghrelin ( ghrelin Inhibitory activation changes

In order to indirectly determine the in vivo activity of the food ingredients, the cell proliferation ability and the content of ghrelin according to the pH change of the food ingredients under acidic, neutral, and basic conditions were investigated (FIG. 3). When the sample was treated with pH change, the proliferative capacity of HUVEC cells was the most stable at pH 7. HUVEC cells showed a slightly weaker differentiation of 60-92% in the pH 2 and pH 10 treatments, but maintained a degree of differentiation around 90% in all the treatments at pH 7.

The residual amount of ghrelin was similar to that of the temperature treatment because the residual amount of ghrelin was lower than that of the control in all the treatments even when pH 2, 7 and 10 were changed in the food ingredients. From this, it was indirectly determined that the appetite suppression effect was maintained overall by suppressing ghrelin activity even when ingesting food components in the body.

Claims (5)

Ascorbic acid (ascorbic acid), hesperetin (hesperetin) and ginsenoside-gg (ginsenoside-Rg1) composition containing one or more components selected from the group consisting of an active ingredient. The appetite suppressing composition of claim 1, wherein the appetite suppressant is an effect of inhibiting the activity of ghrelin, which is an appetite-stimulating hormone. According to claim 2, wherein the appetite suppressing composition, characterized in that the ghrelin inhibitory activity is maintained at a temperature of 60 ~ 100 ℃. The composition for suppressing appetite according to claim 2, wherein the composition maintains ghrelin inhibitory activity at pH 2 to 10. Functional food for suppressing appetite containing ascorbic acid (ascorbic acid), hesperetin (hesperetin) and at least one component selected from the group consisting of ginsenoside-Rg1 (ginsenoside-Rg1) as an active ingredient.

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