KR101230971B1 - Method for Preparing the Bio Polymer Inkjet Ink Composition Based on the Physiological Saline Solution - Google Patents

Abstract

The present invention relates to a biopolymer inkjet ink composition which can use a photocurable inkjet print system, and specifically, a biocompatible polymer inkjet ink composition capable of inkjet printing based on physiological saline containing biogrowth growth factors, keratin and collagen. It is about. The present invention comprises the steps of selecting an food coloring; Preparing a polymer particle composed of three layers; Mixing the food coloring material with the polymer particles, and then irradiating light to form a reversible gel; Preparing a polymer particle dispersion stock solution by mixing the reversible gel and physiological saline containing a biogrowth factor; And it relates to a method for producing a biopolymer inkjet ink composition comprising the step of mixing the dispersion stock and the ink constituent natural substances.

Description

Method for preparing the Biopolymer Inkjet Ink Composition Based on the Physiological Saline Solution}

The present invention relates to a biopolymer inkjet ink composition which can use a photocurable inkjet print system, and specifically, a biocompatible polymer inkjet ink composition capable of inkjet printing based on physiological saline containing biogrowth growth factors, keratin and collagen. It is about.

Biocompatible materials can be divided into artificial organs such as heart tissue and heart valves and hard tissues such as bone and skin, or hard tissues such as skin or therapeutic tissues such as soft and contact lenses. The development of technology for living tissue is being studied mainly on heart and heart-related materials as the minimum material necessary for organ transplantation through prolonging the life of a person who needs organ transplantation.

Biocompatible artificial materials are mainly used for therapeutic materials such as skin, bone or heart valves. In the study of animal tissues similar to humans, research is carried out to form organs in animals similar to humans by proliferating cells by In-Vivo (Virus) or DNA (Gene, Genome) methods through transplantation of nucleus cells. Doing. However, although research is being conducted on time and space issues such as ethical issues and the need to clone animals to replace human organs, they have various problems in reality. In contrast, since 2008, biocompatible artificial materials have been researched by Hewlett-Packard and Columbia Medical School to make biocompatible artificial organs with three-dimensional inkjet printers.

Early work with inkjet inks was conducted by Hewlett-Packard in 2002 and succeeded in creating artificial skin regeneration tissue for burn patients using biomaterials. However, since the two-dimensional printer can manufacture only a part constituting the human body, research on manufacturing human regenerated tissue using a three-dimensional printer has been made. In order to form an artificial organ using a three-dimensional printer, it is necessary to form an artificial organ in a vertical plane, and it is excellent in biocompatibility and utilization because various environmental factors such as blood and stomach acid of humans and human like animals (eg, pigs) must be considered. Large silicon ranges are mainly used. However, viruses can cause artificial material surface infections.

In order to solve this problem, a solvent-free photocurable ink has attracted attention. Photocurable inkjet prints use light (radiation, ultraviolet light, visible light, infrared light, microwaves, etc.) to prevent secondary infection of artificial materials against viruses. However, in the case of using a photocuring system, it is difficult to completely prevent the generation of harmful chemicals depending on the type of the biological ink composition. As a hazardous product of ink, about 10,000 kinds of chemicals are produced by harmful organic volatile and water air pollutants. The International Chemical Safety Card includes acetonitrile, dimethyl sulfate, Dimethyl sulfoxide (DMSO), diethanolamine, N, N-dimethylformamide, formaldehyde, hydrazine, morpholine, methyl ethyl ketone, sodium hydroxide : NaOH), Tetrahydrofuran (THF) and Urea are defined as hazardous chemicals. In addition, the Korea National Institute of Environmental Sciences defines methyl ethyl ketone, trimethyl amine, ethyl acetate, hydrazine, sodium hydroxide, dimethyl sulfate and formaldehyde as hazardous substances.

The use of the harmful organic solvent is for improving the output quality of the ink in the inkjet printing process. Specifically, organic solvents containing such harmful chemicals are used to make an emulsion solution in which the monodispersity index of the biomaterial ink and the biocompatible food pigment (replace the melamine pigment of the human body) particles have high stability. In addition to harmful effects on workers, users and recipients in the process and use, by-products of the printing process can lead to human rejection or to secondary infection by viruses.

It is an object of the present invention to provide a method for producing an ink composition that can be used in the preparation of biomaterials and can be used as an optical crosslinking system ink. The ink composition of the present invention can prevent human body rejection or secondary infection by viruses using natural materials, and can form a three-dimensional organ in a short time using a photocuring system.

An object of the present invention is to provide a method for producing an inkjet ink composition for living tissue without releasing harmful substances. An object of the present invention is to provide an ink composition that is excellent in biocompatibility and minimizes the formation of harmful by-products based on physiological saline containing a biogrowth factor, keratin or collagen, and can be used in a three-dimensional printer.

According to a suitable embodiment of the present invention to solve the above problems, selecting an food coloring; Preparing a polymer particle composed of three layers; Mixing the food coloring material with the polymer particles, and then irradiating light to form a reversible gel; Preparing a polymer particle dispersion stock solution by mixing the reversible gel and physiological saline containing a biogrowth factor; And it provides a method for producing a biopolymer inkjet ink composition comprising the step of mixing the dispersion stock and the ink constituent natural materials.

According to another suitable embodiment of the present invention, the polymer particle is a media layer made of one or more selected from the group consisting of a core layer made of silicon, hydroxyethyl acrylate, hydroxyethyl methacrylate and isopropyl acrylamide, And it is characterized by consisting of a shell layer consisting of one selected from the group consisting of gelatin, factin and cellulose.

According to another suitable embodiment of the present invention, the biogrowth factor is TGF-α, TGF-β, PDGF, TNF-α, bFGF, cytokine IL-1, cytokine IL-6, keratin, collagen, collagen degradation It is characterized in that at least one selected from the group consisting of enzymes, fibronectin and laminine (Laminine).

The biopolymer ink composition prepared in the present invention can be used for various types of heads without using harmful chemicals. In addition, the biomaterial ink composition does not emit harmful chemicals to the curing process, users, or transplantation patients, and uses a three-dimensional inkjet printer to replace artificial nucleus transplants or genes with artificial organs that are less likely to undergo secondary reactions due to human rejection or viruses. It can be produced in a relatively short time than the cloning technique.

1 schematically illustrates a manufacturing process of a biopolymer particle ink composition according to the present invention.
2 shows a block diagram of an optical crosslinking system according to the present invention.
3 is a schematic diagram showing an inkjet printing process using a biopolymer ink according to the present invention.
Figure 4a is a photograph of a living tissue prepared in the present invention.
Figure 4b is another picture of the biological tissue produced in the present invention.

The present invention is based on physiological saline containing biogrowth growth factors and keratin and collagen, and has excellent biocompatibility, high-oxygen permeability, and bio-material for the manufacture of 3D printer biomaterial ink for suppressing formation of harmful by-products in the human environment such as gastric acid. COMPATIBILITY An ink having an organic-inorganic hybrid polymer ink and a food coloring to provide a color similar to a human skin pigment and a material capable of transplanting DNA with biocompatibility and capable of self-replication are prepared.

In order to prevent human rejection and secondary infection by viruses, biopolymer ink particles encapsulated in three layers were formed. The biopolymer ink particle includes a core layer, a media layer, and a shell layer. In order to prepare biopolymer ink particles, a reversible gel polymerization method using photocrosslinking was used. At this time, natural sodium alginate and chitosan, which are mainly used as biooxidation-reducing agents, were polymerized through a ring-open reaction by free electron transfer.

In the polymer particles, the core is used to improve durability when forming a biological tissue using silicon. The media layer is also used as a therapeutic biomaterial and consists of hydroxy polyethylacrylate, ethylmethacrylate or copolymer of isopropylacrylamide and vinyl alcohol with good oxygen and blood permeability. The shell layer is made of gelatin, factin, Or natural polysaccharides such as cellulose.

In general, in the case of artificial body tissues, due to low oxygen permeability, the human body rejection reaction occurs when human waste is implanted inside the exhaust body. In order to prevent this, a polymer particle having a nanoporous structure should be prepared. For this purpose, ligand formation is performed by isopropylacrylamide, which is a heat-sensitive material, when the polymer particle is formed. In addition, it is preferable to use polyvinylpyriridone, which is mainly used as a wound treatment agent, to control the size of the pores by the temperature rise to release sweat or wastes out of biological tissues and to suppress harmful active oxygen activity.

The inkjet ink composition for the photocurable biocompatible tissue material is preferably used by selecting at least one food coloring material such as red, blue, yellow, and black based on physiological saline to make melamine color of the human body.

The ink composition prepared in the present invention, the surface tension is 20 to 70 dyne / cm; Viscosity is 5.0-500 cPs; And it is preferable that the pH is 5 to 10, the surface tension is 20 to 50 dyne / cm; More preferably, the viscosity is 20.0 to 50.0 cPs and pH 6 to 8.

Hereinafter, with reference to the accompanying drawings will be described in detail the present invention.

1 schematically illustrates a process for producing an ink composition according to the present invention.

Referring to Figure 1, the manufacturing process of the ink composition according to the present invention comprises the steps of selecting the food coloring material (S11); Preparing a biopolymer ink particle (S12); Preparing a reversible gel after mixing the food coloring material and the biopolymer particles (S13); Preparing a polymer particle dispersion stock solution using physiological saline (S14); Preparing a biopolymer ink composition (S15); Microfiltration step (S16); It includes a light crosslinking print step (S17). The specific process in each step is as follows.

1. Food coloring step ( S11 )

Since the pigment forms a biopolymer and is applied to the human body, food coloring should be used. Food colorings are red, yellow, blue, and black depending on pH and temperature. The food coloring body according to the present invention may include the following natural materials for each ink.

blue

Gardenia blue pigment, spirulina blue pigment

Red

Anthraquinone: Cochinyl, Calcon, Anthocyanin: Red cabbage, Purple sweet potato

yellow

Carotenoids: Gardenia yellow pigment

black

Squid ink

White

Refined Titanium Dioxide

2. Biopolymer Particle Manufacturing Step ( S12 )

1 selected from the group consisting of hydroxy ethyl acrylate-co-silicone resin, hydroxy ethyl methacrylate-co-silicone resin and isopropylacrylamide-co-silicone resin under standard conditions (atmospheric pressure 1ATM, temperature 298.16 K). Species (Mw: 300,000), polyvinyl alcohol (Hydroxy value: 99.999%, Mw: 300,000), gelatin, polyvinylpyriridone (PVP-40, Mw 40,000) are added. Instead of gelatin, it is possible to use factin or cellulose. Instead of hydroxyethyl methacrylate, polyethylene glycol (Poly Ethylene Glycol), polylactide acid (Poly Lactide Acid), polylactide glycolide (Poly Lactide Glycolide), PLGA (Poly (Lactic-co-Glycolic acid) Polycarprolactone can be used.

Next, 0.1N acetic acid aqueous solution to which chitosan and sodium alginate were added as a photocrosslinking initiator is added. A biopolymer particle composed of a core layer, a media layer, and a cell layer is prepared by a ring-open reaction of nucleophilic free electrons generated through a chitosan oxidation-reduction reaction.

In the biopolymer particles, the core is made of silicon to improve durability in the living body. The media layer is at least one selected from the group consisting of hydroxy ethyl acrylate, ethyl methacrylate or a copolymer of isopropyl acrylamide and vinyl alcohol. The cell layer is made of one selected from the group consisting of gelatin, factin and cellulose.

3. Food coloring and biopolymer ink particle mixing step ( S13 )

The biopolymer particles prepared above are mixed with a food pigment of red, blue, yellow or black, and stirred for 3 to 4 hours to form a blend solution. Light (ultraviolet ray: 253nm-50mJ, visible ray: 473nm, 515nm, 630nm-250mJ, infrared ray: 1504nm, 2500nm-150mJ) is added to the blend solution to prepare a gel stock solution. It is prepared with Degassing using 99.999999999% nitrogen gas from Airproducts during the preparation of the gel stock solution.

4. Biopolymer Particle Reversible Gel Dispersion Solution Preparation Step (S14)

The biopolymer particle reversible gel must first be short dispersed to form a size that can be sprayed through a printer nozzle when composed of inkjet ink. As a dispersion solvent for dispersing the biopolymer particle reversible gel, it is preferable to use a physiological saline containing a biological growth factor, keratin or collagen.

The biological growth factors include TGF-α, TGF-β, platelet-derived growth factor (PDGF), Tumor Necrosis Factor-α (TNF-α), basic fibroblast growth factor (bFGF), cytokine IL-1, IL- 6, keratin, collagen, collagen degrading enzymes (MMP1, MMP2), it is preferable to use at least one selected from the group consisting of extracellular substrates fibronectin (Fibronectin) and laminine (Laminine). In addition, substances that are not allergenic and carcinogenic may be selected, as set out in the European Oeko-Tex Standard 100 regulations. The biological growth factor is preferably added 3 ~ 10mg based on 1L physiological saline.

KOH acid number (Acid number) 50, Amine acid value (Acid number) Polyglycerol fatty acid ester having a density of 100 g and a density of 1.2 g / cm 3 was added to the physiological saline containing the biogrowth factor and sufficiently stirred. Then, the biopolymer particle reversible gel was added and stirred slowly. In the above, it is preferable to mix 20 to 40 wt% of the biopolymer reversible gel with food coloring matter and 60 to 80 wt% of the physiological saline containing the biogrowth factor. The viscoelastic emulsion prepared above is dispersed in a uniform stirring for 20 minutes to 1 hour at 5000 ~ 7000RPM IKA T-50 homogenizer to prepare a biopolymer particle dispersion stock solution. It is manufactured with Degassing using 99.999999999% nitrogen gas from Airproducts during the manufacturing process.

5. Biopolymer Particle Composition Preparation Step (S15)

Titanium dioxide, a photocuring reaction chemical, is added to the biopolymer particle dispersion stock solution prepared in the above step (2 mg / Kg). Titanium dioxide is used as a light absorbing material and serves to accelerate the rate of tissue regeneration, and is completely separated and extracted in the following purification process.

In addition, photochemically reacting natural substances are added to improve the stability, dispersibility, or binding properties of the biopolymer ink composition. The material to be added should improve ink properties such as surface tension, viscosity, antioxidant (pH) and storage stability and at the same time be harmless to humans and the environment.

The additive material is an antioxidant solution containing rosemarine, retinol, Vitamin A, Vitamin C, etc .; Thickeners consisting of xanthan gum, guar gum and the like; And an emulsifier consisting of lecithin, sucrose fatty acid ester, and the like. Finally, the prepared biopolymer ink composition may contain 20-40 wt% of a biopolymer particle reversible gel dispersion stock solution; 0.1 to 1 wt% of a solution for preventing oxidation (pH); Food coloring matter 0.1 to 1 wt%; Thickener: 0.1 to 1 wt%; Redox agent: 0.1 to 1 wt%; Emulsifier 0.5-10 wt%; And 50-60 wt% of physiological saline including a biogrowth factor.

The biogrowth factor may be selected according to the type of biological tissue to be manufactured by the printing process. Specifically, at least one selected from the group consisting of TGF-α, TGF-β, platelet-derived growth factor (PDGF), Tumor Necrosis Factor-α (TNF-α), and basic fibroblast growth factor (bFGF) is preferable. Can be used.

The photoreactant, the dispersion stock solution, is mixed and blended using a mechanical stirrer and prepared with Degassing using 99.999999999% nitrogen and helium gas from Airproducts during the blending process. For example, a variable conditional reaction and storage vessel can be used that is controlled by IKA's computer.

7. Precision filtration step ( S16 )

The prepared ink composition performs a microfiltration process to remove impurities generated or mixed in the manufacturing process and to filter out particles having a predetermined level or more. The filtration process uses ultrafiltration (less than 1 μm), fine filtration (less than 200 nm), selective ultra-precision filtration (less than 100 nm) using Millpore's products, and filtration with a reduced pressure (-1 ATM) vacuum pump. . Through the filtration process, the ink composition has an overall uniform and stabilized particle size.

8. Printing  step( S17 )

The ink composition of which the filtration step is completed may be manufactured by a printing process using a print system equipped with an optical crosslinking system. For example, the optical cross-linking device may use GE's therapeutic far-infrared device (band: 1500-2500 nm) and therapeutic ultraviolet-UV (UV-B) device (band: 200-305 nm). The photocrosslinker accelerates the curing speed of the ink. 2 is a block diagram of an optical crosslinking system according to the present invention.

3 is a schematic view of an inkjet printer of a biopolymer ink according to the present invention.

3, the ink composition 12 of the present invention is ejected through the inkjet head 11 of the printing system to which the photocuring device is attached. At this time, the saline solution 14 including the biogrowth factor is filled in the rectangular glass container 13, and the polyethylene terephthalate film 15 is immersed therein, so that the ink composition 12 is a polyethylene terephthalate film 15. It is printed on the) to form the biological tissue (16).

Manufacturing example -Polymeric biopolymer particles including food coloring and Reversible Gel  Dispersion Stock Preparation

200 g of polyhydroxyethyl acrylate / ethyl methacrylate / isopropylacrylamide-co-silicone resin (Mw: 300,000, manufactured by Sigma-Aldrich, 128635, I22404) under standard conditions (atmospheric pressure 1 ATM, temperature 298.16 K) 100 g of vinyl alcohol (Hydroxy value: 99.999%, Mw: 300,000), 50 g of gelatin, and 30 g of polyvinylpyrilidone (PVP-40, Mw 40,000) are added thereto. After adding 10 g / L of chitosan and 10 g of 0.1N acetic acid solution to which 10 g / L of sodium alginate was added as a photocrosslinking initiator, biopolymer particles are formed through a ring-open reaction of nucleophilic free electrons. The polymer particles form three layers of particles consisting of a core layer, a media layer, and a shell layer.

The core layer is made of a copolymer of silicon, the hydroxy polyethyl acrylate, ethyl methacrylate or isopropyl acrylamide and vinyl alcohol, and the shell layer is made of gelatin. 10 g of one pigment selected from the group consisting of red, blue, yellow and black food colorings are added to the mixture containing the polymer particles and stirred for 3 hours to prepare a suspension. Irradiating the prepared suspension with infrared light to prepare a biopolymer reversible gel stock solution in a gel state. 20 to 40 wt% of the prepared biopolymer particle reversible gel stock solution is added to the physiological saline containing 60 ~ 80wt% containing a growth factor to prepare a dispersion solution while stirring slowly.

The physiological saline is a Sigma-Aldrich containing biological growth factors (TGF-α, TGF-β, platelet-derived growth factor (PDGF), TNF-α (Tumor Necrosis Factor-α) and bFGF (Basic fibroblast growth factor) Products: W3515) 5mg / L, keratin 10mg / L, collagen 20mg / L, collagen degrading enzymes (MMP1, MMP2) 2mg / L, and one of the extracellular matrix Fibronectin or Laminnine Contains 30 mg / L. An appropriate biogrowth factor may be selected according to the type of living tissue to be finally printed. Manufactured with Degassing using 99.999999999% nitrogen gas from Airproducts during the entire manufacturing process.

Example  Biopolymer Particle Ink Composition with 1-Blue Food Color

In the Preparation Example 1 was added blue food coloring to prepare a blue biopolymer particle dispersion stock solution. 300 g of biopolymer particle-dispersed undiluted solution containing blue food coloring vitamin 10 g vitamin A, 10 g xanthan gum, 10 g retinol, and 100 g natural lecithin, 570 g saline solution (platelet-derived growth factor (PDGF) 5 mg / L, TNF-α as a growth factor) (Tumor Necrosis Factor-α) 5 mg / L and bFGF (basic fibroblast growth factor) 5 mg / L, keratin 10 mg / L, collagen 20 mg / L, collagen degrading enzyme (MMP1) 2 mg / L, fibronectin, an extracellular matrix And 30 mg / L) to prepare a blue biopolymer ink composition.

The prepared ink composition was filtered using a member filter. The resulting ink had a surface tension of 38 dyne / cm; Viscosity 18.9 cPs; And pH 7.1. Injecting the ink into the cartridge and using the printing equipment such as Stylus Pro 4800 from Epson, Designer jet z2100 from Hewlett Packard, Canon IPF 600 from Canon, etc. A polyethylene terephthalate film was placed in a glass square container having an A4 area and a 30 mm height filled with physiological saline, and printed on the film to form an artificial cornea, which is a soft tissue of human organs.

Example  Biopolymer Particle Ink Composition with 2-Red Food Pigment

Red food pigment was added in Preparation Example 1 to prepare a red biopolymer particle dispersion stock solution. 320g of biopolymer particle dispersion containing red food pigment, 10g of vitamin A, 10g of xanthan gum, 10g of retinol, and 100g of natural lecithin, 550g of physiological saline (platelet-derived growth factor (PDGF) 5mg / L, TNF-α) (Tumor Necrosis Factor-α) 5 mg / L, bFGF (Basic fibroblast growth factor) 5 mg / L, keratin 10 mg / L, collagen 20 mg / L, collagen degrading enzyme (MMP2) 2 mg / L and extracellular matrix Fibronectin (including 30 mg / L) was mixed to prepare a red biopolymer ink composition. Ink compositions are blended in a mechanical stirrer and prepared with Degassing using 99.999999999% nitrogen and helium gas from Airproducts during the process.

The prepared ink composition was filtered using a member filter. The resulting ink had a surface tension of 39 dyne / cm; Viscosity 18.6 cPs; And pH 7.3. Injecting the ink into the cartridge and using the printing equipment such as Stylus Pro 4800 from Epson, Designer jet z2100 from Hewlett Packard, Canon IPF 600 from Canon, etc. A polyethylene terephthalate film was placed in a glass square container having an A4 area and a 30 mm height filled with physiological saline, and printed on the film to form artificial skin, which is a soft tissue of human organs.

Example  Biopolymer Particle Ink with 3-Yellow Food Coloring

In Yellow Preparation Example 1, yellow biopolymer particle dispersion stock solution was prepared. Biopolymer particle dispersion containing yellow food coloring 310g, vitamin C 10g, xanthan gum 10g, retinol 10g, and natural lecithin 100g, physiological saline 560g (platelet-derived growth factor (PDGF) 5mg / L, TNF-α as a growth factor) (Tumor Necrosis Factor-α) 5 mg / L, bFGF (basic fibroblast growth factor) 5 mg / L, keratin 10 mg / L, collagen 20 mg / L, collagen degrading enzyme (MMP2) 2 mg / L, and extracellular matrix Fibronectin (including 30 mg / L) was mixed to prepare a yellow biopolymer ink composition.

The ink composition is blended in a mechanical stirrer and prepared with Degassing using 99.999999999% nitrogen and helium gas from Airproducts during the process. The prepared ink composition was filtered using a member filter. The resulting ink had a surface tension of 38 dyne / cm; Viscosity 18.4 cPs; And pH 7.5. Injecting the ink into the cartridge and using the printing equipment such as Stylus Pro 4800 from Epson, Designer jet z2100 from Hewlett Packard, Canon IPF 600 from Canon, and the like, bio-growth factors, keratin and collagen A polyethylene terephthalate film was placed in a glass square container having an A4 area and a 30 mm height filled with physiological saline, and artificial skin, which is a soft tissue of human organs, was formed on the film.

Example  Biopolymer Particle Ink with 4-Black Food Coloring

To prepare a black biopolymer particle dispersion stock solution was added black food coloring in Preparation Example 1. 350 g of biopolymer particle dispersion containing black food coloring, 10 g of vitamin A, 10 g of xanthan gum, 10 g of retinol, and 100 g of natural lecithin, 520 g of physiological saline (TGF-α 5mg / L as a biological growth factor, TGF-β 5mg / L, The assay biopolymer ink composition was prepared by mixing 5 mg / L of platelet-derived growth factor (PDGF), 10 mg / L of keratin, 20 mg / L of collagen, and 30 mg / L of extracellular matrix (Laminnine). Prepared.

Ink compositions are blended in a mechanical stirrer and prepared with Degassing using 99.999999999% nitrogen and helium gas from Airproducts during the process.

The prepared ink composition was filtered using a member filter. The resulting ink had a surface tension of 37 dyne / cm; Viscosity 18.2 cPs; And pH 7.8. Physiology containing biogrowth factors, keratin, and collagen was produced using output devices such as Epson's Stylus Pro 4800, Hewlett Packard's Designer jet z2100, and Canon's Canon IPF 600. A polyethylene terephthalate film was placed in a glass square container having an A4 area and a 30 mm height filled with saline, and printed on the film to form artificial bone, which is a hard tissue of human organs.

Claims (4)

Selecting an edible chromosome;
Preparing a polymer particle composed of three layers;
Mixing the food coloring material with the polymer particles, and then irradiating light to form a reversible gel;
Preparing a polymer particle dispersion stock solution by mixing the reversible gel and physiological saline containing a biogrowth factor; And
Method of producing a biopolymer inkjet ink composition comprising the step of mixing the dispersion stock, vitamin A, xanthan gum, retinol and natural lecithin, and physiological saline containing a biogrowth factor. The method according to claim 1,
The polymer particle is a core layer made of silicon,
A media layer composed of at least one selected from the group consisting of hydroxyethyl acrylate, ethyl methacrylate and isopropyl acrylamide, and
Method for producing a biopolymer inkjet ink composition, characterized in that consisting of a shell layer consisting of one or more selected from the group consisting of gelatin, factin and cellulose. The method according to claim 1,
The biogrowth factor is at least selected from the group consisting of TGF-α, TGF-β, PDGF, TNF-α, bFGF, cytokine IL-1, cytokine IL-6, keratin, collagen, collagenase, fibronectin and laminin A method for producing a biopolymer inkjet ink composition, characterized in that one kind. A method of printing a living tissue after mounting the biopolymer ink composition prepared in claim 1 on an inkjet printer with a photocuring device.

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