KR101200333B1 - Thermal stable endolysin LysBPS13, food composition and feed composition thereof - Google Patents
Thermal stable endolysin LysBPS13, food composition and feed composition thereof Download PDFInfo
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Abstract
Description
본 발명은 박테리오파지 유래의 엔돌라이신에 관한 것으로, 더욱 상세하게는 내열성 특징이 있는 엔돌라이신 LysBPS13에 관한 것이다.
The present invention relates to endolasins derived from bacteriophages, and more particularly to endolisins LysBPS13 having heat resistance characteristics.
엔돌라이신(endolysin)은 박테리오파지(또는, 세균 바이러스)에 의해 인코딩되는 펩티도글리칸 가수분해효소인데, 파지증식의 용균 사이클에서 후기 유전자 발현 동안 합성되며, 박테리아 펩티도글리칸의 분해를 통해 감염된 세포로부터 파생(progeny) 비리온의 방출을 매개한다. Endolisin is a peptidoglycan hydrolase encoded by bacteriophage (or bacterial virus), which is synthesized during late gene expression in the lytic cycle of phage proliferation, and is infected through degradation of the bacterial peptidoglycan. It mediates the release of progeny virions from cells.
엔돌라이신은 β(1,4)-글리코실라제(리소자임), 트랜스글리코실라제, 아미다제 또는 엔도펩티다제의 활성을 보여 항균의 용도로 활용될 수 있는데, 항생물질의 성공과 우월함 때문에 그동안 실제 적용은 활성화되지 못하였다. Endolicin can be used as an antibacterial agent because it exhibits the activity of β (1,4) -glycosylase (lysozyme), transglycosylase, amidase or endopeptidase, due to the success and superiority of antibiotics. In the meantime, the actual application was not activated.
그런데, 다수의 항생물질에 내성을 보이는 세균들의 출현 이후, 엔돌라이신은 새롭게 주목받으며, 인간 감염 병원균의 방지 수단으로 많은 관심이 쏠리고 있다. However, after the emergence of bacteria that are resistant to a large number of antibiotics, endolicin has received new attention, attracting much attention as a means of preventing human infection pathogens.
엔돌라이신은 '효소'와 '항생물질'의 혼성 용어인 '엔지바이오틱스(enzybiotics)'로도 사용되는데, 2001년에 피세티(Fischetti)와 그의 동료들은 처음으로 박테리오파지 C1 엔돌라이신의 치료적 효능을 입증하였다(Nelson et al., 2001). Endolicin is also used as a hybrid term for 'enzyme' and 'antibiotics', 'enzybiotics'. In 2001, Fischetti and his colleagues were the first to treat the bacteriophage C1 endolacin. Efficacy was demonstrated (Nelson et al., 2001).
그 후, 다수의 문헌에서, 특히 그람 양성균에 의한 세균 감염에 대한 대안으로 엔돌라이신이 연구되었다. 스트렙토코쿠스 뉴모니애(Loeffler et al., 2001), 바실러스 안트라시스(Schuch et al., 2002) 및 스타필로코쿠스 아우레우스(Rashel et al, 2007)와 같은 그람 양성 병원균에 대한 다양한 엔돌라이신이 엔지바이오틱스로서 효능이 입증되었다.
Subsequently, in a number of documents, endolicin has been studied as an alternative to bacterial infection, in particular by Gram-positive bacteria. Various Negative strains on Gram-positive pathogens such as Streptococcus pneumoniae (Loeffler et al., 2001), Bacillus anthracis (Schuch et al., 2002) and Staphylococcus aureus (Rashel et al, 2007) Dolysine has been shown to be effective as an engineeriotics.
본 발명에서는 박테리오 파지 유래의 신규의 엔돌라이신을 개발하여 제공하고자 한다.
In the present invention, it is intended to develop and provide a novel endolicin derived from bacteriophage.
상기 목적을 달성하기 위하여, 본 발명은 서열번호 2의 아미노산 서열을 갖는 엔돌라이신 LysBPS13을 제공한다. In order to achieve the above object, the present invention provides an endolisin LysBPS13 having the amino acid sequence of SEQ ID NO: 2.
본 발명에서 새롭게 분리한 엔돌라이신 LysBPS13은 식중독의 원인균 중 하나인 바실러스 속 및 외막의 투과성을 높인 살모넬라와 대장균에 대해 특이적인 용균 작용을 보인다. The newly isolated endolicin LysBPS13 in the present invention shows a specific lytic action against Salmonella and Escherichia coli, which enhance the permeability of the genus Bacillus and the outer membrane, one of the causative agents of food poisoning.
또한, 본 발명의 엔돌라이신 LysBPS13은 아미다제(amidase)의 활성을 가지며, pH 7.5~10.5에서 항균 활성을 보인다. 특히 열(100℃)을 가했을 때도 안정적인 항균활성을 보이는데, 42~45℃에서 최적의 활성을 나타낸다.In addition, the endolicin LysBPS13 of the present invention has an amidase activity and exhibits antimicrobial activity at pH 7.5-10.5. In particular, it shows stable antibacterial activity even when heat (100 ℃) is applied. It shows optimum activity at 42 ~ 45 ℃.
한편, 본 발명에 있어서, 서열번호 2의 아미노산 서열은 바람직하게 서열번호 1의 핵산 서열로 암호화되는 것이 좋다. On the other hand, in the present invention, the amino acid sequence of SEQ ID NO: 2 is preferably encoded by the nucleic acid sequence of SEQ ID NO: 1.
한편, 본 발명의 엔돌라이신 LysBPS13는 바실러스 세레우스(Bacillus cereus) 감염 박테리오파지 BPS13 (KCTC 12061BP) 유래인 것일 수 있다. Meanwhile, the endolicin LysBPS13 of the present invention may be derived from Bacillus cereus infected bacteriophage BPS13 (KCTC 12061BP).
한편, 본 발명은 상기 엔돌라이신 LysBPS13을 함유하는 식품 첨가제 또는 식품 조성물을 제공한다. 상기 식품 첨가제 또는 식품 조성물은 엔돌라이신 LysBPS13을 함유하기만 하면 그 제형에 특별히 국한되지 않으나, 대상 식품은 바실러스 속이나 살모넬라 속 또는 대장균에 의해 오염되기 쉬운 식품이면 더욱 바람직하다. On the other hand, the present invention provides a food additive or food composition containing the endolacin LysBPS13. The food additive or food composition is not particularly limited to the formulation as long as it contains endolacin LysBPS13, but the target food is more preferably a food that is susceptible to contamination by Bacillus, Salmonella or E. coli.
또한, 본 발명은 상기 엔돌라이신 LysBPS13을 함유하는 사료 첨가제 또는 사료 조성물을 제공한다. 상기 사료 첨가제 또는 사료 조성물은 엔돌라이신 LysBPS13을 함유하기만 하면 그 제형에 국한되지는 않으나, 바실러스 속이나 살모넬라 속 또는 대장균에 감염되기 쉬운 가축을 대상으로 하는 사료 첨가제 또는 사료이면 더욱 바람직하다.
The present invention also provides a feed additive or feed composition containing the endolacin LysBPS13. The feed additive or feed composition is not limited to the formulation as long as it contains endolacin LysBPS13, but more preferably feed additive or feed for livestock susceptible to the genus Bacillus, Salmonella or E. coli.
본 발명에서 새롭게 분리한 엔도라이신 LysBPS13은 식중독을 일으키는 원인균 중 하나인 바실러스 속 균주들과 외막의 투과성을 높인(EDTA 처리) 살모넬라와 대장균에 대해 특이적인 용균작용을 보이고, 특히 글리세롤을 포함한 효소 저장 버퍼에 희석해 열(100 ℃)을 가했을 때도 안정적인 항균활성을 보이기 때문에 식품, 의약 및 동물 의약 산업 등에 활용될 수 있다.In the present invention, the newly isolated endolysine LysBPS13 shows specific lytic action against Bacillus sp., One of the causative agents causing food poisoning, and Salmonella and Escherichia coli which have enhanced permeability (EDTA treatment), and especially an enzyme storage buffer including glycerol. It can be used for food, medicine and animal medicine industry because it shows stable antibacterial activity even when diluted with heat (100 ℃).
도 1은 LysBPS13의 아미노산 서열 중 기능을 갖는 두 개의 보존 영역(conserved domain: PGRP, SH3_5)을 나타낸 것이다.
도 2는 정제된 엔도라이신 LysBPS13의 SDS-PAGE 겔 사진 (A)과 LysBPS13에 의한 바실러스 세레우스 ATCC 10876 (지시균)의 흡광도 변화 (B)를 보여준다.
도 3은 여러 가지 온도에서 보관한 후, LysBPS13의 항균 활성 변화를 보여준다.
도 4는 LysBPS13의 아미다제 활성을 보여주는 것으로, 바실러스 세레우스 ATCC 10876에서 추출한 펩티도글리칸에 LysBPS13을 처리한 후, 무람산(muramic acid)의 증가량을 나타낸다. Figure 1 shows two conserved domains (PGRP, SH3_5) having a function of the amino acid sequence of LysBPS13.
2 shows the SDS-PAGE gel photograph (A) of purified endolysine LysBPS13 and the absorbance change (B) of Bacillus cereus ATCC 10876 (indicator) by LysBPS13.
Figure 3 shows the antimicrobial activity change of LysBPS13 after storage at various temperatures.
Figure 4 shows the amidase activity of LysBPS13, after the treatment of LysBPS13 to the peptidoglycan extracted from Bacillus cereus ATCC 10876, it shows the amount of muramic acid (muramic acid).
이하, 본 발명의 내용을 하기 실시예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고, 그와 등가의 기술적 사상의 변형까지를 포함한다.
Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.
[[ 실시예Example 1: One: 엔돌라이신Endolicin LysBPS13LysBPS13 의 of DNADNA , 아미노산 서열 분석 및 분리?정제], Amino acid sequencing and separation and purification]
토양으로부터 분리한 박테리오파지 BPS13의 유전자서열을 분석한 뒤, BlastP (Marchler-Bauer, A., et al., 2011)를 통해, 바실러스 박테리오파지 TP21-L의 N-아세틸무라밀-L-알라닌 아미다제(N-acetylmuramyl-L-alanine amidase)와 매우 높은 유사성을 보이는 834 염기쌍의 열린해독틀(open reading frame)을 찾아내고, 이를 LysBPS13이라 명명하였다. After analyzing the gene sequence of bacteriophage BPS13 isolated from the soil, BlastP (Marchler-Bauer, A., et al., 2011), the N-acetylmuramil-L-alanine amidase of Bacillus bacteriophage TP21-L ( An open reading frame of 834 base pairs showing very high similarity with N-acetylmuramyl-L-alanine amidase was identified and named LysBPS13.
분석 결과, LysBPS13은 서열번호 2의 아미노산 서열을 가지는데, 특성이 잘 알려진 N-아세틸무라밀-L-알라닌 아미다제로, PlyCA(AAP42310.2), Ply511(CAA59368.1), T7 리소자임(AAB32819.1) 과는 다른 아미노산 서열을 가지고 있었다. As a result, LysBPS13 has the amino acid sequence of SEQ ID NO: 2, which is well-known N-acetylmurayl-L-alanine amidase, PlyCA (AAP42310.2), Ply511 (CAA59368.1), T7 lysozyme (AAB32819 .1) had a different amino acid sequence.
보존 영역(Conserved domain)을 분석한 결과, 아미다제 활성을 갖는 펩티도글리칸 인식 단백질(peptidoglycan recognition protein)과 세포에 부착하는 기능을 갖은 SH3_5 슈퍼패밀리(superfamily)를 포함하고 있었다(도 1).
Analysis of the conserved domain revealed that a peptidoglycan recognition protein with amidase activity and the SH3_5 superfamily with the ability to adhere to cells were included (FIG. 1).
[[ 실시예Example 2: 2: 엔돌라이신Endolicin LysBPS13LysBPS13 의 생화학적 특성 분석]Biochemical Characterization of
LysBPS13 단백질을 정제(도 2의 A)한 후, 항균활성을 측정하였는데, pH 7.5부터 pH 10.5 사이에서 항균활성이 나타났으며, pH 9.5에서 가장 강한 활성(용해능, lytic activity)을 보였다. After purifying LysBPS13 protein (A of FIG. 2), the antimicrobial activity was measured. The antimicrobial activity was observed between pH 7.5 and pH 10.5, and showed the strongest activity (lytic activity) at pH 9.5.
또한, 5 μg/ml의 소량으로도 바실러스 세레우스 ATCC 10876을 10분 안에 용해시켜 흡광도가 감소하는 것을 볼 수 있었다(도 2의 B).In addition, even a small amount of 5 μg / ml Bacillus cereus ATCC 10876 was dissolved in 10 minutes it was seen that the absorbance is reduced (B of Fig. 2).
항균활성은 넓은 범위의 온도에서 안정하게 나타났으며, 그 중 최적온도는 42℃와 45℃ 사이였다.
The antimicrobial activity was stable at a wide range of temperatures, and the optimum temperature was between 42 ℃ and 45 ℃.
[[ 실시예Example 3: 3: 엔돌라이신Endolicin LysBPS13LysBPS13 의 항균 활성 범위]Antimicrobial Activity Range of
하기 표 1 및 표 2에서 보이는 것과 같이 여러 가지 박테리아에 대한 항균활성을 측정하였는데, 그 결과, 그람 양성균인 바실러스 세레우스(B. cereus)와 바실러스 서린지엔시스(B. thuringiensis)에 대하여 높은 활성을 보였고, 바실러스 리케니포미스(B. licheniformis), 바실러스 메가테리움(B. megaterium), 바실러스 퍼밀러스(B. pumilus)의 경우에는 비교적 낮은 활성을 보였다. As shown in Table 1 and Table 2, the antimicrobial activity of various bacteria was measured. As a result, high activity against Gram-positive bacteria B. cereus and B. thuringiensis was observed. B. licheniformis , B. megaterium and B. pumilus showed relatively low activity.
그람 음성균의 경우, 외막의 투과성을 높이기 위해 EDTA를 처리한 박테리아에 대한 활성을 측정하였는데, 살모넬라(S. Typhimurium) SL1344, UK1, 14028s, DT104)와 대장균(E. coli) DH5α, DH10B에 대해 높은 활성을 나타냈다. 하지만, 비브리오(V. fiischeri)의 경우, 항균 활성을 띄지 않았다. In the case of Gram-negative bacteria, activity against EDTA-treated bacteria was measured to increase permeability of the outer membrane, which was high for S. Typhimurium SL1344, UK1, 14028s, DT104) and E. coli DH5α, DH10B. Activity was shown. However, Vibrio ( V. fiischeri ) did not show antimicrobial activity.
[[ 실시예Example 4: 4: 엔도라이신Endolysine LysBPS13LysBPS13 의 내열성 측정]Measurement of heat resistance]
LysBPS13의 내열성을 알아보기 위해 4~100℃ 사이의 온도에서 보관한 뒤, 효소활성을 측정하고자 하였다. 해당 온도에서 각각 보관한 후, 각각의 효소를 효소 저장 버퍼(20mM Tris-Cl, 300 mM NaCl, 30% Glycerol)에 희석하고, 활성을 측정하였다. To determine the heat resistance of LysBPS13 was stored at a temperature between 4 ~ 100 ℃, to measure the enzyme activity. After each storage at that temperature, each enzyme was diluted in enzyme storage buffer (20 mM Tris-Cl, 300 mM NaCl, 30% Glycerol) and the activity measured.
그 결과, 4~70℃에서는 엔도라이신에 가해진 온도와 관계없이 열처리를 하지 않은 LysBPS13과 같은 항균활성을 보였다(도 3). As a result, at 4 ~ 70 ℃ exhibited the same antimicrobial activity as LysBPS13 without heat treatment irrespective of the temperature applied to the endor lysine (Fig. 3).
한편, 80, 90, 100℃에서는 열처리를 하지 않은 대조군에 비해 활성이 떨어지기 시작했으나, 그래도 각각 60 %, 40 %, 15 % 이상의 활성을 유지하였다.
On the other hand, at 80, 90, 100 ℃ activity began to drop compared to the control group without heat treatment, but still maintained the activity of 60%, 40%, 15% or more, respectively.
[[ 실시예Example 5: 5: 엔도라이신Endolysine LysBPS13LysBPS13 의 of 아미다제Amidase 활성 측정] Activity measurement]
본 실시예에서는 LysBPS13의 아미다제 활성을 조사하였다. 아미다제 활성은 N-아세틸무람산(N-acetylmuramic acid)로부터 유리된 락틱 그룹(lactic group)을 측정하는 방법을 통해 평가되었다. In this example, the amidase activity of LysBPS13 was examined. Amidase activity was assessed by measuring the lactic group liberated from N-acetylmuramic acid.
실험 결과, 정제된 LysBPS13을 바실러스 세레우스 ATCC 10876에서 추출한 펩티도글리칸에 처리하였을 때, 펩티도글리칸의 N-아세틸무람산과 L-알라닌(L-alanine) 사이의 아미드(amide) 결합이 끊김으로써, 유리 무람산(muramic acid)이 증가됨을 알 수 있었다(도 4).As a result, when the purified LysBPS13 was treated with peptidoglycan extracted from Bacillus cereus ATCC 10876, the amide bond between N-acetylmuramic acid and L-alanine of peptidoglycan was broken. As a result, it was found that free muramic acid was increased (FIG. 4).
이 결과는 LysBPS13이 박테리아 세포벽에 있는 펩티도글리칸층의 아미드 (amide) 결합을 절단하는 아미다제(amidase) 활성을 가짐을 보여준다.
These results show that LysBPS13 has an amidase activity that cleaves amide bonds of the peptidoglycan layer on the bacterial cell wall.
[[ 실시예Example 6: 6: LysBPS13을LysBPS13 함유하는 Containing 식품 첨가제의Of food additives 제조] Produce]
표고버섯 분말 50중량%, 새우 분말 30중량%, 다시마 분말 15 중량%, 본 발명의 엔돌라이신 LysBPS13 5중량%를 혼합하여 조미료로 사용될 수 있는 식품 첨가제 300g을 제조하였다.
50% by weight of shiitake powder, 30% by weight of shrimp powder, 15% by weight of kelp powder, and 5% by weight of endolicin LysBPS13 of the present invention were mixed to prepare 300 g of a food additive that can be used as a seasoning.
[[ 실시예Example 7: 7: LysBPS13을LysBPS13 함유하는 식품의 제조] Production of Food Containing]
<우유 제조> <Milk production>
시판 중인 S 제조의 우유 200 mL에 본 발명의 엔돌라이신 LysBPS13을 1%(w/v) 첨가하여 본 발명에 따른 우유 조성물을 제조하였다.
A milk composition according to the present invention was prepared by adding 1% (w / v) of the endorlicin LysBPS13 of the present invention to 200 mL of commercially available S-produced milk.
<바나나 쥬스 제조> Banana juice production
바나나를 믹서에 넣고 갈아 바나나 원액 180 mL를 준비한 후, 본 발명의 엔돌라이신 LysBPS13을 1%(w/v) 첨가하여 본 발명에 따른 바나나 쥬스 조성물을 제조하였다.
After the bananas were put in a mixer to prepare a 180 mL banana stock solution, 1% (w / v) of endolicin LysBPS13 of the present invention was added to prepare a banana juice composition according to the present invention.
[[ 실시예Example 8: 8: LysBPS13LysBPS13 을 함유하는 사료 첨가제 제조] Preparation of feed additives containing
α-토코페롤 5.0중량%, 오징어간유 0.75중량%, 효모 93.25중량%, 본 발명의 엔돌라이신 LysBPS13 1.0중량%를 혼합하여 사료 첨가제를 1 kg을 제조하였다.
1 kg of a feed additive was prepared by mixing 5.0% by weight of α-tocopherol, 0.75% by weight of squid liver oil, 93.25% by weight of yeast, and 1.0% by weight of endolicin LysBPS13 of the present invention.
[[ 실시예Example 9: 9: LysBPS13을LysBPS13 함유하는 사료의 제조] Preparation of Feed Containing]
< 육우 사료조성물 제조> <Beef feed composition manufacturing>
육우용 사료조성물을 제조하였다. 기본사료는 미가공옥수수 5 kg, 면실피 펠렛 1.5 kg, 생미강 0.5 kg, 소맥피1.5 kg, 면실박 0.45 kg, 대두박 1.0 kg, 소금 0.1 kg, 석회석 0.15 kg, 당밀 0.25 kg, 미량광물질 0.05 kg으로 조제되었다. 여기에 본 발명의 엔돌라이신 LysBPS13을 0.1 kg 첨가하였다.
A feed composition for beef cattle was prepared. The basic feed is 5 kg of raw corn, 1.5 kg of cotton thread pellets, 0.5 kg of fresh rice bran, 1.5 kg of wheat buckwheat, 0.45 kg of cotton bean paste, 1.0 kg of soybean meal, 0.1 kg of salt, 0.15 kg of limestone, 0.25 kg of molasses, and 0.05 kg of trace minerals. Prepared. To this was added 0.1 kg of endolicin LysBPS13 of the present invention.
<산란계용 사료조성물 제조> <Manufacturing feed composition for laying hens>
본 발명의 엔돌라이신 LysBPS13 1.0%(w/w)를 시판 산란계용 사료(디럭스 산란(제품명), 부국사료, 한국)에 첨가하여 산란계용 사료조성물 1 kg을 제조하였다.
Endolysine LysBPS13 1.0% (w / w) of the present invention was added to a commercial laying hen's feed (deluxe egg laying (product name), Bukook feed, Korea) to prepare a feed composition for laying hens 1 kg.
<넙치 양식용 사료조성물 제조><Furniture composition for halibut farming>
어분 58중량%, 대두박 4중량%, 콘글루텐분 3중량%, 소맥분 28.7중량%, 오징어간유 4중량%, 비타민 혼합제 1.2중량% 및 미네랄 혼합제 1중량%를 포함한 기본 사료에 본 발명의 엔돌라이신 LysBPS13 1.0중량%를 첨가하여 넙치 양식용 사료조성물 1 kg을 제조하였다.
Endolysine of the present invention in a basic feed comprising 58% by weight of fish meal, 4% by weight of soybean meal, 3% by weight of gluten meal, 28.7% by weight of wheat flour, 4% by weight of squid liver oil, 1.2% by weight of vitamin mixture and 1% by weight of mineral mixture 1 kg of LysBPS13 was added to prepare 1 kg of aquaculture feed composition.
서열목록 전자파일 첨부Attach an electronic file to a sequence list
Claims (7)
Endolicin LysBPS13 consisting of the amino acid sequence of SEQ ID 2;
상기 서열번호 2의 아미노산 서열은,
서열번호 1의 핵산 서열로 암호화되는 것을 특징으로 하는 엔돌라이신 LysBPS13.
The method of claim 1,
The amino acid sequence of SEQ ID NO: 2,
Endolicin LysBPS13, which is encoded by the nucleic acid sequence of SEQ ID NO: 1.
상기 엔돌라이신 LysBPS13은,
바실러스 세레우스(Bacillus cereus) 감염 박테리오파지 BPS13 (KCTC 12061BP) 유래인 것을 특징으로 하는 엔돌라이신 LysBPS13.
The method of claim 1,
The endolicin LysBPS13,
Bacillus cereus (Bcillus cereus ) infection bacteriophage BPS13 (KCTC 12061BP) characterized in that the endolacin LysBPS13.
A food additive containing endolicin LysBPS13 consisting of the amino acid sequence of SEQ ID NO: 2.
A food composition comprising an endolisin LysBPS13 consisting of the amino acid sequence of SEQ ID NO: 2.
A feed additive containing endolicin LysBPS13 consisting of the amino acid sequence of SEQ ID NO: 2.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101571835B1 (en) * | 2013-01-18 | 2015-11-25 | 서울대학교산학협력단 | Novel endolysin from bacteriophage SPN1S and antibiotic composition thereof |
| KR101785487B1 (en) | 2016-06-30 | 2017-10-16 | 경희대학교 산학협력단 | Endolysin lysbps13, and uses thereof |
| KR102035283B1 (en) | 2019-01-31 | 2019-10-22 | 서울대학교 산학협력단 | Thermal stable endolysin LysCPD2 with lytic activity against Clostridium perfringens |
| KR102097128B1 (en) * | 2019-06-19 | 2020-04-06 | 서울대학교산학협력단 | Chimeric endolysin Lys109 with antimicrobial activity against Staphylococcus aureus |
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2011
- 2011-12-05 KR KR1020110129087A patent/KR101200333B1/en active IP Right Grant
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101571835B1 (en) * | 2013-01-18 | 2015-11-25 | 서울대학교산학협력단 | Novel endolysin from bacteriophage SPN1S and antibiotic composition thereof |
| KR101785487B1 (en) | 2016-06-30 | 2017-10-16 | 경희대학교 산학협력단 | Endolysin lysbps13, and uses thereof |
| KR102035283B1 (en) | 2019-01-31 | 2019-10-22 | 서울대학교 산학협력단 | Thermal stable endolysin LysCPD2 with lytic activity against Clostridium perfringens |
| KR102097128B1 (en) * | 2019-06-19 | 2020-04-06 | 서울대학교산학협력단 | Chimeric endolysin Lys109 with antimicrobial activity against Staphylococcus aureus |
| WO2020256268A1 (en) * | 2019-06-19 | 2020-12-24 | 서울대학교 산학협력단 | Chimeric endolysin lys109 which effectively controls staphylococcus aureus |
| US11827912B2 (en) | 2019-06-19 | 2023-11-28 | Seoul National University R&Db Foundation | Chimeric endolysin LYS109 with antimicrobial activity against staphylococcus aureus |
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