KR101167999B1 - Composition for preventing and treating diabetes comprising the extract of big cone pine needle - Google Patents

Abstract

The present invention relates to the use and use of pine needles extract in regulating blood sugar and cholesterol.

Extract according to the present invention inhibits weight loss due to deficiency of insulin secretion in pancreatic cells, not only lowers blood sugar, but also improves lipid metabolism and suppresses renal function, thereby exhibiting an excellent anti-diabetic effect, preventing or Effective for treatment Therefore, it can be prepared from safe therapeutics, health foods, health functional foods and food raw materials.

Diabetes disease, blood sugar control, cholesterol control, pine needle extract

Description

COMPOSITION FOR PREVENTING AND TREATING DIABETES COMPRISING THE EXTRACT OF BIG CONE PINE NEEDLE}

The present invention relates to the use and use of pine needle extract in regulating blood sugar and cholesterol for diabetes.

Diabetes is a disease that occurs in humans and mammals and is caused by abnormal glucose levels in plasma. Diabetes mellitus is characterized by two types: insulin dependent type I diabetes and insulin independent type II diabetes. Type I diabetes is caused by a lack of insulin, a glucose-regulating hormone in the blood, and is often referred to as pediatric diabetes because it occurs mainly in young people in their 10s and 20s. Type II diabetes is caused by subtle changes in the insulin secretion of the pancreas due to poor pancreatic insulin secretion or tissue rejection of insulin. Type II diabetes occurs mainly after the age of 40 and is classified as adult diabetes, but both types can occur at any age. About 90% of Korean diabetics have type II diabetes. Currently, there are about 2.6 million diabetic patients in Korea, 8% of the national population, and the number of diabetic patients is increasing by 260,000, 10% of the total number of patients each year. In addition, diabetic patients are three times more likely to die than those without diabetes.

The pine tree belongs to the pine family (the scientific name Pinus koraiensis SIEB), and the pine leaf is also called pine leaf. According to the data of pine leaves, the pine leaves contain essential oils such as terpene, phenolics, and tannins, and various components such as chlorophyll, inorganic and organic ingredients, and vitamins. have. Pine nuts are known as Haesongja and have the effects of nutrient solution, bogie, and yang blood, but there is no oriental medical record of the efficacy or use of pine needles. Consent). Recently, pine needle tea using pine needles (Korean Patent No. 10-0638062, published on October 24, 2006), clean rice containing the components of pine needles (Korean Patent 10-2003-0062189, published on July 3, 2003), pine needle noodles Products such as pine needles, pine needles vinegar, and pine needles are sold, suggesting the possibility of expanding the use of pine needles as food ingredients. Pines belonging to the same family as pine tree have been used for various medicines depending on the harvesting area (medicine plywood). According to recent research data, pine leaves have angina, myocarditis, arrhythmia, cerebral infarction, hyperlipidemia, arteriosclerosis, and senile dementia. And it is known that it is effective in the treatment of sudden deafness, and the pine tree is also a natural resource foreseen for the purpose of preventing and treating adult diseases. We have found that pine needle extract can be used to regulate blood sugar and cholesterol.

The present invention relates to the treatment and prevention of diabetes as a novel use of pine needle extract extracted from pine needles.

The present invention includes a process and a process for extracting the pine leaves collected for this purpose.

The present inventors have made no efforts to develop a preventive and therapeutic agent for diabetes by using a safe natural agent, and as a result, the pine needle extract confirmed that the anti-diabetic activity in streptozotocin-induced diabetic rats was completed. The present invention, after all, relates to its use in regulating blood sugar and cholesterol for diabetic disease. The present invention also provides a pharmaceutical composition for controlling blood glucose and cholesterol for diabetic disease.

In order to achieve the above object, the present invention obtains the extract through washing, drying, cutting and pulverizing, extraction and concentration, aging step pine nuts. In the present invention, the extract can be extracted from the pine leaves in a conventional manner. For example, there are water extraction method, water + alcohol extraction method, alcohol extraction method, steam distillation extraction method. The preferred method is steam distillation extraction.

As an example, the present inventors will explain in detail a method of preparing a pine leaf extract using a steam distillation extraction method.

Collect fresh pine needles, remove sick, insect bites, and dry ones. Remove the selected pine needles into a washing tank and wash them three times with running water. The dried pine needles are shaded for 3 days, and they are constantly cut to about 10 mm using a cutter. The cut pine is transferred to a distillation tank. Put the ratio of pine needles and purified water in the distillation tank at about 1: 2, and then distilling the pine tree leaves in the distillation tank at 90 ° C for 3-4 hours. At this time, the temperature of the cooling water supplied into the cooler is about 18 ° C, and the condensate passing through the cooler is collected in the condensate separator. The collected condensate is a mixture of oil and water, which is then concentrated and filtered to obtain a high purity extract. The obtained extract has a pine-colored aroma with clear colorless and transparent oil. The final extract obtained during the extraction process is aged for 30 days in the dark place for balance (stabilization), flavor and aroma of the final chemical composition.

The pharmaceutical composition of the present invention may be delivered by oral administration or topical application. These include, for example, tablets, solutions for oral administration, powders, pellets, capsules, injections (including intravenous injections), lotions for external application, Gao, tinctures, grinds and infusions. It may be prepared in various kinds of dosage forms such as infusion and the like. In oral administration, the pine leaf extract is administered three times a day for 0.3-1.5 g per adult, preferably 0.4-0.5 g per serving, and 0.3- per serving for adolescents in 10-20 years. 0.5 g three times daily.

Diabetes is difficult to cure and can control blood sugar by diet, exercise and drug therapy, but natural drugs are preferred because of the side effects of continuous use of synthetic drugs. The present invention has a satisfactory effect to control the blood sugar and cholesterol for the diabetic patient is a natural product can be useful as an effective and safe medicine and health functional food for the prevention or treatment of diabetes diseases.

Pine trees are widely distributed in Korea, eastern Siberia, Manchuria, and the Japanese archipelago, so they can secure raw materials at low cost. In Korea, the major forest planting species accounting for 31% of Korea's artificial forests in recent decades. 87% of ghost trees under 30 years of age, including artificial plantations, require long-term care of pine trees. Therefore, the present invention is easy to secure the raw materials, such as the thinning of the reforestation pine forest, the use of discarded pine tree leaves can be emerged as a new income resources of forest farmers and contribute to the income increase of forest farmers, and consequently the economic benefits and the national It is a breakthrough that is expected to contribute greatly to health improvement.

Example 1 Checking the Effect of Lowering Blood Sugar

   The experimental animals purchased male rats of Sprague-Dawley (SD) species weighing 180 ± 10 g (Samtaco Bio Korea, Korea), temperature 23 ± 3 ℃, relative humidity 60 ± 10%, ventilation 10 -15 times / hr. It was allowed to acclimate for one week in a 12-hour contrast cycle of 150-300Lux at roughness, and water and feed (Agreen Brand, Purina Korea, Korea) were freely consumed. After acclimation, the rats were fasted for 24 hours except for the normal group (G1), and then weighed. Diabetes was induced by intraperitoneal administration of streptozotocin (STZ) of 50 ml / kg after weight measurement, and then, after one week of administration, blood glucose was measured and only rats of 200 mg / dl or more were selected. Grouping In other words, it was divided into the normal control group (NC) and three diabetes-induced groups, and the diabetes-induced group was divided into the diabetes control group (DNC), the pine leaf low-dose group (DLP), and the pine leaf high-dose group (DHP). The birch leaf distillate was administered in 100ml of excipient corn oil and 2g and 5g of pine leaf distillate, respectively, 1ml per 100g of experimental animals, and the control group was oral once daily for a total of 4 weeks. Administered.

      Fasting Blood Glucose Measurement: After fasting for 6 hours, blood from the tail vein was measured using a blood glucose meter (ACCU-CHEK Active, Roche Diagnostics GmbH) and Strips (GLUCOTREND, Roche Diagnostics GmbH).

 Table 1. Effect of Pine Distillate on Fasting Glucose in Streptozotocin-induced Diabetic Rats

(mg / dl)

Experimental group Weeks after oral administration 0 One 2 3 4 Normal 75 ±
4.3 79.2 ±
3.4 80.5 ±
4.0 83.2 ±
5.0 84.7 ±
3.1 Contrast / STZ Group 235.2 ±
60.3 250.7 ±
48.6 320.0 ±
52.2 347.5 ±
31.1 379.0 ±
47.4 Low volume / STZ group 240.4 ±
40.7. 247.5 ±
34.0 221.9 ±
65.0 251.5 ±
39.8 273.8 ±
50.7 High capacity / STZ group 230.2 ±
45.5 220.6 ±
44.0 200.2 ±
55.9 210.8 ±
29.9 220.6 ±
30.6

As a result, the blood glucose level of rats was decreased from 1 week after the administration of test substance compared to the diabetic control group, and at 2 weeks of administration of test substance, there was a significant hypoglycemic effect in the high dose group of pine needle distillate (Table 1).

Example 2 Blood Biochemical Test

4 weeks after the administration of the test substance, blood from the test animal was collected from the posterior vena cava, centrifuged from the collected blood, and serum was collected. The total cholesterol, triglycerides and creatine were analyzed using an automatic analyzer (INTEGRA 400, Roche, Germany). The content of was measured.

 Table 2. Blood Biochemical Characteristics of Diabetic Rats Induced by Streptozotocin

(mg / dl)

Experimental group Total cholesterol Creatine Triglyceride Normal 25.5 ± 3.4 0.3 ± 0.0 40.3 ± 4.0 Contrast / STZ Group 44.3 ± 5.7 0.3 ± 0.0 45.2 ± 5.4 Low volume / STZ group 35.5 ± 5.1 0.4 ± 0.0 23.5 ± 3.8 High capacity / STZ group 31.4 ± 7.4 0.3 ± 0.0 25.9 ± 5.5

As a result, the total cholesterol content was decreased in concentration-dependent manner in the pine leaf distillation group. Triglycerides were low in the low and high dose groups of the test substance (Table 2).

The present invention aims to develop anti-diabetic natural products and to develop health foods, health functional foods and food raw materials based on the material obtained through the extraction process of pine leaf.

The material obtained through the present invention has been shown to have an excellent effect on the treatment and prevention of diabetes and can be used as a material for the treatment of blood sugar control of diabetes patients, health foods for preventing diabetes.

It can be commercialized into individual health functional foods and individual foods through industrial recognition procedures of KFDA which are recognized for specific efficacy for health functional foods, and can be industrialized as functional food raw materials.

In addition, new drug certification procedures can lead to the development of new drugs for the treatment of diabetes.

Glucose decreased the blood glucose of rats from the first week of administration of the test substance compared to the diabetic control group, and showed a significant hypoglycemic effect in the high dose group of pine needle distillate at the second week of the administration of the test substance (Fig. 1).

Claims (6)

As a pharmaceutical composition for the prevention and treatment of diabetes mellitus containing the extract of Pinus koraiensis SIEB as an active ingredient, The pine leaf extract is a pharmaceutical composition which is obtained by the steam distillation extraction method, showing the pine-tree aroma, clear colorless transparent oil. A pharmaceutical for treating diabetes comprising the composition of claim 1 and pine leaf extract as an active ingredient. As a health functional food for diabetes prevention and symptom relief containing pine nut extract as an active ingredient, The pine leaf extract is a health functional food obtained by the steam distillation extraction method, showing the pine-tree aroma, clear colorless transparent oil extract. It is a food for diabetes prevention and symptom relief containing pine tree leaf extract as an active ingredient, The pine leaf extract is a food extract, which is obtained by the steam distillation extraction method, showing the pine-tree aroma, clear colorless transparent oil. The method of claim 1, wherein the pine leaf extract The cut pine needles were placed in a distillation tank at a ratio of 1: 2 with purified water, and then distilled at an extraction temperature of 90 ° C. for 3 to 4 hours, and passed through a cooler to collect condensate. The collected condensate was concentrated and filtered. Pharmaceutical composition obtained by. The method according to claim 3 or 4, wherein the pine leaf extract The cut pine needles were placed in a distillation tank at a ratio of 1: 2 with purified water, and then distilled at an extraction temperature of 90 ° C. for 3 to 4 hours, and passed through a cooler to collect condensate. The collected condensate was concentrated and filtered. Food obtained by.

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