KR101158039B1 - Immune-Boosting Composition Comprising Immuned Beestings and Immuned York - Google Patents

Abstract

The present invention relates to immunopotentiating compositions comprising immune colostrums and immune yolks immunized with specific antigens, and more particularly, the present invention relates to immune colostrums and immunizations comprising specific antibodies capable of reducing major diseases occurring in farms. It provides powdered complex colostrum prepared by producing egg yolk and collecting, preserving, drying and sterilizing it.

The immunopotentiating composition of the present invention can significantly increase the specific antibody titer in the blood of the axial axis to a high level and significantly reduce the disease of the new axial axis and increase the immunogenicity.

Description

Immune-Boosting Composition Comprising Immuned Beestings and Immuned York}

The present invention relates to an immuno-enhanced composition comprising a combination of immune colostrum and immune yolk containing the desired immunoglobulin, and more specifically, to a complex colostrum composition to which colostrum and egg yolk dermis extract, etc. immunized with a specific antibody are added, and a method for producing the same. It is about. The present invention also relates to a method of feeding the composition to enhance immunity of young animals.

Calf diarrhea is the most prevalent disease worldwide, with the highest rate of calf mortality. The average mortality of calves is at least 5-25%, depending on the conditions of specification management. Calf diarrhea, pathogenic infections such as bacteria, viruses, parasites, poor quality substitutes, overdose of milk, ingestion of indigestible carbohydrates and coarse fertilizers, sudden changes in milk, cold, poor warmth, and foreign body intake , Long distance transport, psychological stress, mastitis of the mother, obesity of the mother, malnutrition of the mother.

In addition, the respiratory disease of cows, when the respiratory system mucosa is damaged by dense breeding, stress caused by group breeding, polluted air, etc., the axial axis weakens the basic immune system, so the anti-morbidity falls. Recombinant infection of bacteria or viruses in a degraded biological environment results in respiratory illness. The microbiological agents of respiratory diseases are mainly various bacteria and viruses. The major bacterial causes are Pasteurella, Hephilus and Mycoplasma. Viral causes include bovine infectious rhinitis virus (IBRV), bovine viral diarrhea virus (BVDV) and bovine parainfluenza virus (PI-3). ) And microvesicle pneumonia virus (BRSV).

On the other hand, in the case of newborn calves, the intestinal mucosa and respiratory mucosa are insufficient due to the lack of sufficient immunoglobulin A (IgA) that can be primarily defended in the intestinal or respiratory mucosa if colostrum does not provide sufficient passive immunity from the mother. Destruction occurs. This makes secondary infections easier on the mucous membrane and puts the calf in serious condition. Therefore, it is key to reduce calf mortality by ensuring that the mother's colostrum is adequately ingested so that primary mucosal immunity caused by IgA can be exerted and prevented from developing sepsis or viremia caused by immunoglobulin G (IgG). Tizard, Ian R., 2004). To this end, in order to increase calf immunity immediately after delivery and before and after weaning, by feeding colostrum with sufficient amounts of specific antibodies to newborn cows, maintaining high antibody value in calf blood increases the resistance to disease, making it difficult to develop digestive or respiratory diseases. Not only that, but mixed infections can also be prevented.

The cows secrete colostrum, which allows for a smooth growth of their cattle for about a week after delivery. In particular, compared to regular milk, colostrum contains a large amount of physiologically active factors that play an important role in biological defense functions such as immune factors, growth factors, wound healing factors, and antibacterial factors (Buttler, 1971, 1986. Pakkanen and Aalto, 1997). ). In addition, colostrum is a rich nutrient source for newborns, which increases DNA synthesis and cell division activity, establishes the immune system of the newborn, and promotes its activity to prevent infection of bacteria and viruses in the digestive tract (Wilson, 1997; Schams, 1994).

Representative immune substances in colostrum are IgG, IgM contained in the blood and IgA, which is mainly present in mucosal tissues and is responsible for direct defense (Buttler, 1994). In addition, Insulin-like Growth Factor (IGF-I, II), Epidermal Growth Factor (EGF), Transforming Growth Factor (TGF), and Platelet-Derived Growth Factor (PDGF) are typical growth factors in colostrum. (Butler, 1994; Regester et al., 1997).

On the other hand, as time passes after delivery, the immune component of the cow's superfluid drops sharply from 50% within 6 hours to 12% after 20 hours, and the rich nutrients and immunofactors in colostrum also change over time after delivery. Decreased (Gregory S., Kelly, ND, 2003). In particular, newborn cows do not secrete digestive enzymes only during the first 24 hours of birth, and they absorb the immune substances in colostrum through the gut wall of the digestive tract as it is. Tizard, Ian R., Schubot, Richard M., 2004). Therefore, thorough observation and treatment of the calf will help the mammary colostrum to be delivered within 30 minutes of the calf's birth, or by artificial breastfeeding, a sufficient amount of colostrum (1.0-1.5l) should be obtained within 24 hours at the latest. It must be fed.

On the other hand, as in the mammal's immune system, chickens protect the chicks from invading harmful pathogens by transferring immune antibodies to chicks born through eggs. That is, the antibody in the serum of the mother chicken is transferred into yolk (york immunoglobulin; IgY), which becomes the immune antibody of developing chicks and newly hatched chicks (Akita, EM and Li Chan, ECY, 1998).

In particular, the related field 'Field evaluation of chicken egg yolk immunoglobulins specific for bovine rotavirus in neonatal calves' reported the effect of immunoglobulin in egg yolk on the treatment of rotavirus, a major causative agent of diarrhea in calves, and' Chicken Egg Yolk Antibodies as Therapeutics in Enteric Infectious Disease reported that immunoglobulins in egg yolk are effective against gut infections such as E. coli, yeastia, corona, and salmonella in addition to bovine rotavirus, and that immune yolk will play a significant role in the future infection of animals and humans. .

In particular, laying hens are easier to breed on a larger scale than mammals and can produce an average of 285 eggs per year per breeder. It contains 8 ~ 20mg of IgY per ml of egg yolk, and the weight of one egg yolk is about 15 ~ 17g, so the total egg yolk globulin (IgY) per egg is 136 ~ 340mg and more than 30g of IgY per year from one laying hen. Can be. The amount of IgG that can be produced from 100 ml of colostrum collected on the first day is 3-4 g, so that the amount of IgY that can be produced from egg laying water per year through egg yolk is about 10 times that of IgG in colostrum (Sim, J. S. 2003).

Injection of a specific antigen into the laying hens yields eggs containing specific antibodies, and the processed immune yolks can produce specific IgY against E. coli, Salmonella and certain viruses. Therefore, it can be applied to the prevention and treatment of certain diseases occurring in humans and animals, and can also be used as a substitute for colostrum of new growth.

In order to solve the disease and mortality problems of newborn cattle, when a vaccine inactivated several specific antigens that induce digestive and respiratory diseases and cause fatal course is administered to cows and laying hens before delivery, Because of the formation of specific antibodies in the body, delivery cows produce colostrum containing high titers of immunoglobulin (Ig) and laying hens produce immune eggs containing high titers of immune yolk globulin (IgY). After drying, processing and sterilizing specific immune colostrum and egg yolk, make powdered complex colostrum, and if they are urgently given to new livestock within 3 hours after birth, high titer of immune substance is transferred to the livestock, which causes disease and mortality of new livestock. The present invention has been made to identify a method that can improve the preservation efficiency and dramatically increase the preservation and usability, and to complete the present invention.

It is intended to provide an immunopotentiating composition comprising immune colostrum and immune yolk immunized with a specific antigen of the present invention.

In addition, the present invention is to provide a method for producing an immuno-enhancing composition to produce the immune colostrum and immune yolk immunized with a specific antigen, drying and processing it.

In addition, another object of the present invention is to provide a product produced by the above method and a method of feeding the same, and a method of boosting the animal through the same.

In order to achieve the above object, the present inventors, for example, when a vaccine inactivated several specific antigens, which mainly cause digestive and respiratory diseases and cause fatal course, is administered to cows and laying hens before delivery, Because specific antibodies are formed, delivery cows produce colostrum containing high titers of immunoglobulin (Ig), and laying hens produce immune eggs containing high titers of immune yolk globulin (IgY). We have developed a method for making powdered complex colostrum that is easy to feed to newborn calves by collecting, drying, processing, bottling, and sterilizing these specific immune colostrums and immune yolks.

In addition, in the present invention, by adding the dermis and xylitol, which can dramatically increase digestive absorption and palatability, and urgently feed them to the new livestock within 3 hours after birth, high titers of immune substances are transferred to the livestock, resulting in disease and mortality of the new livestock. It was confirmed that can be efficiently improved.

That is, the present invention provides an immunopotentiating composition containing immune colostrum obtained from a mother mammal immunized with a vaccine and an immune yolk obtained from a mother bird. The vaccine may also be a vaccine against a microorganism selected from the group consisting of E. coli, Salmonella, bovine rotavirus, bovine corona virus, bovine virus mucosal virus, Pasteurella multocida and Pasteurella hemoranitica.

In addition, the immune colostrum and the egg yolk of the composition of the present invention may be composed of an appropriate blending ratio as necessary, preferably, each weight ratio may be included in 1: 1.

In addition, the composition of the present invention may be prepared in powder, granule, liquid, stagnate or capsule form, and preferably in powder form. In addition, the composition of the present invention may be prepared as a pharmaceutical composition, food, food additives or health functional food.

In addition, the composition of the present invention may be added grapefruit seed extract for the purpose of preservation, for example, may be added at a concentration of 500 ~ 1000ppm.

In addition, the composition of the present invention may be further added dermis, peony or licorice extract for the purpose of digestion or analgesic. For example, the extracts may be added 1 ~ 5g per 10g of colostrum, respectively.

In addition, the composition of the present invention may be further added to xylitol for the purpose of sweetening or inhibition of fermentation, for example the xylitol may be added 1 to 5% by weight.

On the other hand, the present invention provides a method of enhancing the immunity of an individual by feeding the composition of the present invention. In particular, the salary preferably comprises within 12 hours of delivery.

In addition, the present invention comprises the steps of (a) vaccinating a specific microbial antigen to the mother animal to obtain immune colostrum and eggs; And (b) mixing the immune colostrum and egg to prepare an immuno-enhancing composition. In particular, the microorganisms include Escheria Coli, Salmonella, Bovine rota virus, Bovine corona virus, Infectious bovine rhinotrichitis (IBR), Bovine vira disease -mucosal disease (BVD-MD), Pasteurella multocida, and Pastureella hemolytica. In addition, the immune colostrum can be obtained from mammals, and the immune yolk from birds.

In addition, the composition of the present invention can be dried and prepared in powder form, the production method can be carried out by conventional methods.

Hereinafter, the present invention will be described in detail.

1. Inoculation and Blood Collection of Specific Antigens

The immunopotentiating composition of the present invention includes immune colostrum from the mother mammal immunized with the vaccine and immune yolk from the mother bird. In addition, the vaccines include, but are not limited to, Escheria Coli, Salmonella, Bovine rota virus, Coronavirus, Infectious bovine rhinotrichitis (IBR), Virus It may be a vaccine against a microorganism selected from the group consisting of Bovine vira disease-mucosal disease (BVD-MD), Pastureella multocida and Pastureella hemolytica.

In the present invention, the vaccine may use commercially available commercially available or self-produced antigen. The vaccine method can be carried out according to a conventional method, for example, the attenuated vaccine of the microbial specific antigen was injected into muscle or subcutaneously in cows 2 and 4 weeks before delivery. In addition, the chickens spawning the attenuated vaccine of the specific antigens were injected intramuscularly by 0.1 ml into the chest of the vaccine group laying hens at 3, 6 and 9 weeks. After the first injection and before each injection step 5 ~ 10ml of blood was collected and centrifuged and cryopreserved at minus 20 ℃ after inactivation treatment, the antibody was used for later analysis.

2. Collection and Preservation of Colostrum and Egg Yolk

In the present invention, the collection of colostrum and the collection of egg yolk can be made by conventional methods. For example, the collection of colostrum is carried out for 6, 12, 24 and 36 hours immediately after delivery, and the grapefruit seed extract when collecting samples in 500-1,000 ml plastic bottles [DF-100, Ltd.] FB Bank, Korea)] was added at a concentration of 500 ~ 1,000ppm was shaken and stored at minus 20 ℃, microorganisms and antibodies were later provided for analysis.

On the other hand, for example, egg yolk is collected from three weeks after vaccination, and egg yolks are collected every day, and each egg yolk is collected in one week after refrigeration. The eggshell surface of the egg yolk was washed with tap water and sterilized with 10-fold diluted povidin iodine. After egg yolk was removed, egg whites were removed and only egg yolks were collected and filled into sterilized polyvinyl bags. After grapefruit seed extract was added and shaken with 500 ~ 1,000 ppm, the eggs were preserved at minus 20 ℃, and microorganisms and antibodies were later provided for analysis. It was.

3. Drying Colostrum and Egg Yolk

The colostrum and egg yolk obtained in the present invention may be dried as necessary, such as processing, distribution, and preservation in powder form. For example, the cryopreserved samples were thawed at room temperature by the hot spray drying method of colostrum and egg yolk, and the yolk was pretreated by adding 3 times distilled water, and then provided to the spray drying. The initial discharge temperature of the spray dryer was set to 102 ° C. by spraying, and each sample of Immune colostrum and egg yolk was spray dried separately. Also, samples for antibody titer and microbial evaluation were collected and cryopreserved in a freezer at minus 20 ° C., and later used according to the purpose of the experiment.

4. The antibody of the sample is measured

In the present invention, the antibody titer of the sample may be performed by the following procedure. First, the serum of cryopreserved blood samples was inactivated by treatment at 56 ° C. for 30 minutes, and then antibody titers were measured using an ELISA method. Cryopreserved colostrum and egg yolk samples were thawed first, and then treated with colostrum for 10 minutes at 12,000 rpm / mim. Immune egg yolk was treated with 1 g of egg yolk in 10 ml of distilled water for 10 minutes at 12,000 rpm. The yolk of the liquid was collected, and the antibody titer of each sample was measured using the ELISA antibody measuring method.

The titer of each pathogen of colostrum and egg yolk and the composite colostrum powder of the present invention was measured using an indirect enzyme-linked immunosorbent assay (ELISA).

① 100 μl of E. coli, Salmonella Typhimurum, Corona virus, Rota virus, Bovine Viral Diarrhea virus, diluted in 0.5M sodium carbonate buffer (pH 9.6) was applied to polystyrene wells (Greiner bio-one, America). The plate incubated at 4 ° C. overnight was washed three times with 0.1 M phosphate buffered saline containing 0.05% Tween 20 (PBST, pH 7.4). The wells were blocked with 200 μl 1% gelatine (Sigma, USA) for 1 hour at 37 ° C. and washed three times with PBST.

② Calf serum samples were diluted 10-fold, 100-fold, 1000-fold, 10,000-fold with PBS and put 100μl into wells coated with antigen. After incubation at 37 ° C. for 60 minutes, the cells were washed in the same manner as before, followed by sensitization at 37 ° C. for 60 minutes at 100 μl of horseradishperoxidase-conjugated rabbit anti-bovine IgG (dilution 1: 2000, Sigma, USA).

③ After washing again, OPD (ο-phenylendiamine, Shanghai Chemicals, China) was used as a substrate. After 30 minutes of reaction at 50 μl of 2M H ₂ SO ₄ , absorbance was measured at 490 nm using an EL × 800 Microplate Reader (Bio-Tek Instruments Inc. Winooski, Vermont, USA).

5. Microbial detection of samples

Microorganism detection of the sample in the present invention, for example can be carried out by the following method. In other words, Microbial detection of cryopreserved samples was treated after thawing cryopreserved colostrum and yolk sample, and cultured for 24 hours to measure the total number of bacteria, and cultured in blood medium for 72 hours to determine colonies of general bacteria. For microbial detection of powdered dry, each sample was taken immediately after spray drying and after gamma sterilization. After dilution, the total number of bacteria was measured by culturing for 24 hours, and the colonies of general bacteria were collected by culturing in blood medium for 72 hours. Determined.

The detection target bacteria were limited to Escherichia coli, Salmonella, Pasteurella, Staphylococcus aureus, fungus, Gram-positive bacteria, Gram-negative bacteria, and proteus. In order to observe various microbial changes in the preserved Whey and egg yolk before drying, only whey and egg yolk were isolated from the colostrum produced within 12 hours after the birth of the vaccine mother and the egg laying hens. Mold was sampled and diluted in 0.1% peptone solution at an appropriate magnification to determine the change in mold water according to the APHA method (1985). Mold was measured after incubation for 48 hours at 25 ℃ in Potato Dextrose medium.

6. Mixing and Packaging of Dry Powder Sample

The immunopotentiating composition of the present invention may be prepared in the form of a dry powder. In one example, the blending ratio of the dry powder in the composition of the present invention was determined according to the weight capacity of each of the dried colostrum and the immune egg yolk. The bottling and packaging of the dry powder was added to 100ml PVP bottles with 10g of immunoprepared milk powder, 10g of immune egg yolk, 1g of Xylitol, 3g of vegetable extracts (dermis, peony, licorice) and vitamins.

7. Sterilization of Dry Powder Samples

Sterilization of the bottling dry powder sample was carried out using a gamma sterilizer (JS-8900, Canada), the gamma-ray irradiation was irradiated for 25kg rey, 28 hours.

8. Analysis of Maternal Behavior

An analysis of lactation behavior according to the birthing of Hanwoo was conducted, and as an evaluation of lactation behavior, the allowance of mammals for the livestock was allowed in 'permissible behavior', and the fleeing or indifferent behavior of the livestock was 'avoided behaviour', and the intestines (kicking) and horns were pushed. The acts they make were classified as 'attack actions'.

Maternal behavior analysis was carried out according to the breed of the axial axis, and the evaluation of the mammalian response was 'good' if the intake within 10 minutes without rejection of the total amount prepared after the stimulation of artificial lactation was 'normal', and if it was taken slowly for 10 to 20 minutes 'If the mammalian reaction is weak and the tongue is not pushed out or the colostrum is not swallowed, it is judged as'bad'.

9. How to Feed Colostrum

Artificial lactation was performed at various doses of 50, 100, 500, 1,000, and 2,000 ml over 1, 2, 3, 6, 12, and 24 hours immediately after birth. Immune colostrum supplemented with 1 to 2 liters of colostrum pretreated with artificial milk in artificial breast bottles and artificial breast milk to the livestock. Immune yolk supplementation was preserved after diluting 1 to 3 vaccine yolks diluted with water five times and shaken them.

Feeding of powdered complex colostrum, which is an example of the composition of the present invention, is mixed with a spray-dried colostrum powder and an egg yolk powder, each containing a total amount of 20 g of powder in a bottle and added 1 to 5 times of lukewarm water. After that, artificial lactation was performed.

10. Blood Collection and Blood Analysis

In one example of the present invention, the blood collection time for cows is set before the vaccine, 7-10 days before delivery, within 12, 24 hours immediately after delivery, blood collection, centrifugation, inactivation, sample number 5-10ml in the jugular vein Granted, frozen at minus 20 ℃ and then provided for later experiments.

The blood collection time for the mammalian axis was set at 1, 2, 3, 6-12 hours, 12-24 hours, 24 hours or more before and after mammaling. 5-10 ml was collected from the jugular vein, centrifuged, inactivated and sampled. Numbering, frozen at minus 20 ℃ was provided for later experiments.

The blood collection time for disease axis was 5-10ml in the jugular vein during diarrhea and pneumonia, blood collection, centrifugation, inactivation, sample numbering, and frozen at minus 20 ℃.

Blood collection for laying hens was set up before, 3, 6, 9, and 12 weeks after the first vaccine, and 4-5 ml of subcutaneous veins of 5 to 6 hens were collected, centrifuged, inactivated, sampled, and minus 20. After freezing at ℃ was provided for later experiments.

11. Disease Assessment

Health checkups were performed for 3 weeks after birth and twice a day for 12 weeks. Body temperature and pulse / breathing were measured for 2 weeks, and the evaluation period of disease was limited to digestive and respiratory symptoms until 14 days after birth. It was based on.

Diarrhea was identified as diarrhea, and diarrhea was classified as grade 4 according to the fecal fluidity score of Larson et al. (1977). Pneumonia was determined in cases with parenteral dryness, purulent rhinorrhea, and coughing, and death in the course of one month of disease.

When the disease occurred, 5 ~ 10ml of blood was collected from the jugular vein and the antibody was used for analysis.

The immune enhancing composition of the present invention contains high titers of specific antibodies in immune colostrum and egg yolk, thereby increasing the antihypertensive ability of the neonatal axis, and adding the dermis and xylitol to further promote digestion and increase palatability. In addition, it can be manufactured in a powder form can increase the utilization, it is possible to drastically improve the disease and mortality of the new livestock by urgently feeding in the shortest time after delivery.

1 is a general diagram of a method for evaluating the production process and process of the powdered composition (powdered colostrum) of immune colostrum and immune yolk in accordance with an example of the present invention.
2 is a finished product produced according to the present invention.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited to the following examples.

Experimental Example 1

Antibody titers in blood according to days of vaccination of specific antigens (titer / ml) Vaccine elapsed date
(week, hour) Escherichia coli Salmonella Rotavirus Coronavirus Coronavirus Pasteurella- Multocida Pasteurella-Hemolytica Before vaccination 36 52 64 38 28 44 32 1 week before delivery 736 892 420 648 728 527 696 12h after delivery 954 543 234 526 612 504 756 24h after delivery 979 504 346 576 662 510 686

As shown in Table 1 above, the effect of specific antigen vaccines before and after vaccination on the antibody antibody titers significantly increased the specific antibody titer in the blood of cows before and after delivery compared to the control group (vaccine group). I was.

These results were 2-4 times higher antibody titer than conventional water-soluble vaccines, which may be attributed to the use of antigen purification and enrichment and specific excipients.

<Experimental Example 2>

Serum antibody titers in laying hens according to days of vaccination of specific antigens (titer / g) Vaccine elapsed date
 (week) Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica Before vaccination 640 479 146 36 26 1157 36 3 weeks after vaccination 2018 1400 1499 626 526 2313 1226 6 weeks after vaccination 2183 1727 1701 1284 1096 2511 2402 9 weeks after vaccination 2663 2005 1737 1926 2026 2631 2864

As shown in Table 2 above, the effect of specific antigen vaccine on pre- and post-inoculation laying antibody titers significantly increased the specific antibody titer in the blood of laying hens after inoculation compared to the control group (vaccine group). These results were 20-40 times higher than those of conventional water-soluble vaccines, which may be attributed to the use of antigen purification and enrichment and specific excipients.

<Experimental Example 3>

Antibody titers in colostrum according to the collection time of immuno colostrum (titer / g) Colostrum Collection Time
  (hour) Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica 1 ~ 6h after delivery 2446 1573 368 628 1024 1953 2016 6 ~ 12h after delivery 1673 910 470 496 965 840 1806 More than 24h after delivery 1633 653 272 512 944 634 1824

As shown in Table 3, the effect of the collection time of the immuno- colostrum was confirmed that it rapidly decreases as time passes after delivery. Therefore, it was confirmed that the collection of immune colostrum is preferably made within 6 hours after delivery.

<Experimental Example 4>

Antibody titer in egg yolk according to collection time of egg yolk (titer / g) Egg yolk collection
(week) Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica Before vaccination 1253 980 254 32 28 1311 126 3 weeks spawning 2303 1616 412 612 724 2100 862 6 weeks spawning 2512 2001 581 1126 1236 2178 1228 9 weeks spawning 2993 2201 812 1262 1538 2298 1652

As shown in Table 4 above, it was confirmed that the effect of the collection time of the egg yolk on the antibody titer was maintained for 9 weeks after spawning.

<Experimental Example 5>

Microbial Detection Phase in Cryopreserved Immune Colostrum According to Preservatives (Water / ml) Preservative Total bacteria count / ml Escherichia coli Salmonella Staphylococcus aureus fungi Gram-negative bacilli Gram-positive bacilli Control colostrum 256,200 5,980 - - - 192,400 6,800 Grapefruit Additive Colostrum 175,600 1,220 - - - 62,800 1,253

As shown in Table 5, the effect of the preservatives added to the cryopreserved immune colostrum on the growth of microorganisms, the grapefruit seed extract added as a preservative to the immune colostrum showed a significant growth inhibitory effect in the total number of bacteria compared to the control group.

<Experimental Example 6>

Microbial detection phase in cryopreserved immune yolk sac according to preservatives (water / ml) Preservative Total bacteria / ml Escherichia coli Salmonella Staphylococcus aureus fungi Gram-negative bacilli Gram-positive bacilli Control egg yolk 52,800 - - - - 36,200 6,400 Grapefruit Additive Yolk 240 - - - - 96 -

The effect of preservatives added to cryopreserved immune yolk yolk on the growth of microorganisms, as shown in Table 6 above, was significantly reduced in the total bacterial count and suppressed the growth of pathogenic microorganisms compared to the control group. Showed an effect

Experimental Example 7

Antibody Colostrum Antibodies According to the Drying Method of Immune Colostrum (titer / g) Drying method Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica Adsorption drying 604 1060 274 56 42 645 68 Spray drying 2446 1594 848 928 1126 2299 1886

As shown in Table 7 above, the effect of the drying method of immune colostrum on the antibody titer in powdered colostrum was slightly decreased in the spray drying method, but there was no significant difference from the adsorption drying group.

<Experimental Example 8>

Antibody Value of Immune Yolk by Drying Immune Yolk (titer / g) Drying method Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica Adsorption drying 64 52 50 38 62 54 42 Spray drying 2462 1963 1525 1326 1128 2481 1926

As shown in Table 8, the effect of the egg yolk drying method on the antibody titer in the powder yolk was slightly decreased in the spray drying method, but there was no significant difference from the adsorption drying group.

Experimental Example 9

Antibody Value of Powdered Complex Colostrum According to Mixing Ratio (titer / g) Compounding ratio (cold milk: egg yolk)
(g: g) Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica 1: 2
(7: 13) 17,122 / 32,006 11,158 / 25,519 5,936 / 19,825 6,612 / 10,428 8,662 / 16,960 16,093 / 32,253 15,560 / 28,600 1: 1
(10: 10) 24,460 / 24,620 15,940 / 19,630 8,480 / 15,250 8,862 / 14,200 7,990 / 12,200 22,990 / 24,81, 14,860 / 15,600 2: 1
(13: 7) 32,798 / 17,234 20,722,13,741 11,024 / 10,675 12,260 / 6,856 16,680 / 15,620 29,887 / 17,367 29,860 / 14,600

As shown in Table 9 above, the effect of the blending ratio of dry powder on the antibody value of the powdered complex colostrum was the minimum antibody in the group where the ratio of colostrum and egg yolk was 1: 1 or more when the capacity of the dry powder was 20. Corresponds to the potency level. Since the colostrum antibody value is more than four times that of egg yolk, there is an antibody value corresponding to 50 g of powdered yolk, and 50 g of powdered yolk corresponds to 10 liquid yolks. This is the amount of 3-4 g of immunoglobulin present in 100 ml of colostrum.

Experimental Example 10

Detection of Microorganisms in Powdered Colostrum with Sterilization Method (Water / ml) Sterilization Total bacteria
/ ml Escherichia coli Salmonella yellow
Staphylococcus fungi Gram-negative bacilli Gram-positive bacilli Control group
(Spray drying) 0 89,600 - - - 72,800 15,600 Gamma Sterilization
(Spray drying) 0 - - - - - -

As shown in Table 10, the effect of the sterilization method of the powdered complex colostrum was not detected any microorganisms in the control and sterilization group in the spray drying method.

Experimental Example 11

Antibodies in the blood of livestock according to feeding time (titer / g) Payroll time Escherichia coli Salmonella Rota-virus Corona-virus Small Virus Mucosa-Virus Pasteurella- Multocida Pasteurella-Hemolytica Before pay <10 <10 <10 <10 <10 <10 <10 1 ~ 3h after salary 1083 598 717 864 668 632 726 3 ~ 12 after pay 1367 633 473 924 768 866 822 12 ~ 24h after pay 1259 636 660 896 812 856 842

As shown in Table 11 above, the effect of colostrum feeding time on the antibody titer in the blood of the axial axis, the higher antibody level reached the higher the post-natal feeding time of the axial axis.

Experimental Example 12

Morbidity and mortality of livestock according to the type of salary colostrum (%) Colostrum Type disease Our company
(%) Plant
(%) diarrhea
(%) Pneumonia
(%) General super paid benefits
(n = 67) 10
(14.9) 10
(14.9) 7
(10.4) 16
(23.9) Immune yolk salary
(n = 43) 2
(4.7) 3
(7.0) 2
(4.7) 7
(16.3) Immune colostrum benefits
(n = 52) 5
(9.6) 4
(7.7) One
(2) 4
(7.7) Combined Collateral Benefits
(n = 35) 0
(0.0) One
(2.9) 1 (2.9) 2
(5.7)

As shown in Table 12, the effect of the type of fed colostrum on morbidity and mortality of porcelain was significantly decreased in the group of Immune Colostrum, Yolk Colostrum, and Combined Colostrum.

As described above, according to the present invention, the immuno-enhancing composition of the present invention can significantly reduce morbidity and mortality of the axial axis containing highly specific antibody titers and can be prepared in powder form, thereby dramatically improving its availability and functionality. You can.

Claims (12)

Contains a mixed colostrum formulated with a 1: 1 by weight ratio of immune colostrum from the mother mammal and immune yolk from the mother bird, immunized with the vaccine,
Immuno-enhancing composition, characterized in that 10 to 20% by weight and 1 to 5% by weight of xylitol extract selected from the dermis extract, peony extract or licorice extract relative to the total weight of the complex colostrum. The method of claim 1,
The vaccine is a vaccine against a microorganism selected from the group consisting of Escherichia coli, Salmonella, bovine rotavirus, bovine corona virus, bovine virus mucosal virus, Pasteurella multocida and Pastureella hemoranitica. . The method of claim 1,
A composition, characterized in that the grapefruit seed extract is further added. The method of claim 3,
The grapefruit seed extract is characterized in that the composition is added to 500 ~ 1000ppm. delete The composition of claim 1 wherein the composition is in powder, granule, liquid, stagnate or capsule form. delete delete delete delete delete delete

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