KR101152910B1 - Processfood of a cherry tomato for improving a prostate function and the manufacturing method - Google Patents

Abstract

The present invention relates to processed cherry tomato foods to enhance the function of the prostate, and a method for manufacturing the same, and has the same effect as saw palmetto by adding a component that can increase the content of lycopene, and also enhance the function of the prostate and improve liver function. It is intended to provide a drop tomato processed food and a method of manufacturing the same to enhance the prostate function, which is easy to carry and drink.

That is, in the present invention, after harvesting and washing the organically grown cherry tomatoes, 100% by weight of naturally dried and ground cherry tomatoes are first mixed with 0.05% olive oil by weight ratio of 300 seconds and brown sugar in a weight ratio of 49:51. 1% by weight of the fermented stock solution of 300 seconds prepared by mixing fermentation and 0.1% by weight of snail chondroitin prepared by lyophilizing the dried snail and pulverized powder with sodium acetate solution were mixed, and the mixed solution was introduced into a dual pressure steamer to obtain 1.5 The present invention was prepared by the method of freeze-drying the concentrated liquid prepared by heating for 1 hour under a pressure of kgf / ㎠ and 100 ~ 110 ℃ temperature to the concentrated tomato powder.

Cherry Tomato, Processed Foods, Lycopene, Prostate Function Improvement, Liver Function Improvement

Description

Processed cherry tomatoes for improving a prostate function and its manufacturing method {Processfood of a cherry tomato for improving a prostate function and the manufacturing method}

The present invention relates to processed cherry tomato foods to enhance the function of the prostate, and a method for producing the same, more specifically, to increase the content of lycopene, and to enhance the prostate function and improve the liver function by adding a saw palmetto and It is intended to provide a processed cherry tomato foods and a method of manufacturing the same to have the same effect, to enhance the prostate function is easy to carry and drink.

The technology known as tomato or cherry tomato processed foods relates to a method of preparing tomato juice, a salted tomato juice and a manufacturing method thereof (Domestic Patent Registration No. 181004), a method of producing cherry tomato juice, and cherry tomato juice produced by the same. (Domestic Patent Registration No. 865131) and the like, and these prior arts are techniques for manufacturing juice.

Saw palmetto (saw plmetto), on the other hand, is a saw-shaped leaf palm tree that has long been eaten by the Indians living in Florida for men's health and has since become widely known as Europeans came to the United States to see and consume it.

Recent studies have shown that the ingredients extracted from the green fruits of saw palmetto contain abundant phytonutrients such as B-carotene, b-sitosterol and saponin, and the active ingredients, fatty acids and sterols, contain the prostate gland. Although it is widely used to suppress the production of a hormone called hyperhydrogen dihydrotestosterone and inhibit the action to improve the urination problem, it is widely used, but has a disadvantage of being expensive.

In the present invention, while reducing the discomfort associated with the portability and drinking of the conventional cherry tomato processing food to increase the lycopene content, and also to enhance the prostate function and improve the liver function by adding ingredients such as saw palmetto The present invention has been completed with the technical object of the present invention to provide a drop tomato processed food and a method for producing the same to enhance the prostate function that may have.

In the present invention, after washing and washing the organically grown cherry tomatoes, 100% by weight of naturally dried and ground cherry tomatoes, 0.05% by weight of olive oil, and then three hundred seconds and brown sugar ratio of 49:51 1% by weight of the fermented stock solution of 300 seconds by mixing and fermentation with 0.1% by weight of snail chondroitin prepared by lyophilizing the dried snail and pulverized powder with sodium acetate solution, and putting the mixed solution into a dual pressure steamer The present invention was prepared by the method of freeze-drying the concentrated liquid prepared by heating for 1 hour under a pressure of 1.5kgf / ㎠ and 100 ~ 110 ℃ temperature to produce a concentrated tomato product.

According to the cherry tomato processed food prepared in the present invention, after the cherry tomato is heated at a high temperature, it is supplied as a frying dry bar, which increases the content of lycopene, which acts as an antioxidant among the cherry tomato ingredients, and is better absorbed by the human body. Can be.

Since the fermentation of three hundred seconds fermented juice known to enhance male genital function and snail chondroitin known to improve liver function is added, the present invention is expected to help improve the prostate gland and tired liver function in men It is expected to contribute greatly to health promotion.

It will be described below cherry tomatoes processed food and its manufacturing method for enhancing the prostate function provided by the present invention.

The present invention is a freeze-dried processed food added with a small amount of olive oil used to increase the lycopene content of cherry tomatoes and cherry tomatoes as the main ingredients, and a 300 seconds fermentation stock solution for improving the prostate gland of men and snail chondroitin for improving tired liver function to be.

The cherry tomato contains a lot of lycopene (Lycopene) which is a good ingredient for the body. Lycopene originates from the tomato species and lycopersicum (mostly Lycopersicon esculentum), a potent antioxidant that acts as an anti-cancer effect and prevents active oxygen, a major component of cancer.

One common misconception is that tomato tomatoes are completely nutritious because they are completely different from regular tomatoes, but the amount of lycopene contained in cherry tomatoes surpasses that of regular tomatoes.

As a result of analyzing lycopene content of general tomato and cherry tomato at Buyeo tomato test site, it was 26% higher than that of mature tomato.

Tomatoes are better vegetables when heated. Lycopene, a healthy ingredient, is fat-soluble, so lycopene, which is an antioxidant contained in tomatoes, is better absorbed by the body when heated.

In a university study, tomatoes were heated at 88 ° C. for 2, 15, and 30 minutes, but the vitamin C levels decreased by 10%, 15%, and 29%, respectively, compared to before heating, but the amounts of lycopene were 6% and 17, respectively. % And 35% increase, and it is known to increase the absorption rate of lycopene when fried in olive oil (oil).

Olive oil serves to increase the content of lycopene when cooked when heating tomatoes as described above.

The three hundred seconds fermentation stock solution was prepared by mixing the three hundred seconds and brown sugar in a weight ratio of 49:51 and fermenting the same, according to the 'method of producing a three hundred seconds fermentation stock solution for improving male reproductive function' of the present patent application No. 0508539. Use what was prepared.

And snail chondroitin is a technique for obtaining the extract of chondroitin by taking the supernatant after aging the growth or growth of edible snails by lyophilized powder with sodium acetate solution at pH 4-5, 33-36 ℃ for about 20-24 hours The present invention is provided by the technique described in Japanese Patent No. 0720344 filed by the inventor.

The controitin is a polysaccharide consisting of N-acetylgalactosamine-uronic acid (glucuronic acid or iduronic acid) -sulfuric acid, which is known as a main component of cartilage, and is also contained in various connective tissues such as skin, umbilical cord, and granulation. It is natural that a variety of commercially available ones can be selected and used as necessary.

The processed cherry tomato product provided in the present invention after washing and washing organically grown cherry tomato, 100% by weight of naturally dried and crushed cherry tomato juice to 0.05% by weight of olive oil and then three hundred seconds and brown sugar 49: 1% by weight of three hundred seconds fermentation stock solution prepared by mixing and fermenting at a ratio of 51, and 0.1% by weight of snail chondroitin produced by aging dried snail and pulverized powder with sodium acetate solution, and mixing the mixed solution with a dual pressure steam machine. The concentrated liquid prepared by heating to 1 hour under a pressure of 1.5kgf / ㎠ and 100 ~ 110 ℃ temperature was lyophilized and concentrated powder.

The manufacturing method is as follows: 1) harvesting and washing, 2) drying, 3) grinding, 4) additive mixing, 5) steam heating, 6) cooling, 7) freeze drying, 8) packaging. .

1) Harvesting and Washing

Cherry tomatoes are picked from red ones grown organically, harvested, soaked in purified water for 2 hours, and washed. This cleaning can completely remove pollutants (dust, acid rain, etc.) that can naturally bury or remain.

2) dry

Naturally dried cherry tomatoes washed as described above.

As a drying method, it is preferable to dry naturally for about 1 hour using sunlight, but it is natural that drying can be performed using a dryer according to climatic conditions.

3) crushing

Put the washed and dried cherry tomatoes as described above in a grinder to grind.

At this time, it is preferable to grind so that the peel of the tomato is not visible.

4) additive mixing

In the present invention, in order to increase the lycopene content of cherry tomatoes, a small amount of olive oil is added first, followed by the fermentation stock of 300 seconds, which is a component for enhancing prostate function, and snail chondroitin, a component capable of improving liver function.

The mixing ratio of the additive is suitable for 100% by weight of cherry tomato juice, 0.05% by weight of olive oil, 1% by weight of three hundred seconds fermentation stock solution, 0.1% by weight of snail chondroitin.

If you add a lot of olive oil because it smells like oil, pay attention to the above ratio is a ratio that is considered to be appropriate to smell or taste in the sense of smell.

5) steam heating

In the state in which the mixed solution in which the additive is mixed as described above is added to the sack, the mixture is put into a dual pressure steamer, heated at a pressure of 1.5 kgf / cm 2 and at a temperature of 100 to 110 ° C. for about 1 hour, and a concentrated solution is prepared. .

The heating of cherry tomatoes is based on a study by the academic community that vitamin C is destroyed but the anti-cancer effect of lycopene increases.

6) cooling

The concentrated solution obtained by steam heating as described above is cooled to about 20 ° C. at room temperature.

This is to increase the efficiency of freeze-drying to be described later.

7) Lyophilization

The concentrated liquid cooled as described above is dried using a freezing dryer to form a concentrated powder.

At this time, the concentrated powder is dried to close to zero water content so as not to deteriorate in the distribution process.

8) Packing

The concentrated powder produced by the lyophilization may be packaged in capsule form or provided in a compressed ring in a predetermined shape.

In addition, it is possible to provide a powder state as it is in the packaging.

<Experimental Example>

1. Experimental Animals and Diet

Experimental animals received female rats (Sprague-Dawley) from Daegu Hyochang Science for 7 days of age at around 170-180g body weight. The experimental rats were divided into four groups of seven dogs, each of which was divided into four groups according to the egg mass method. The rats were separated into cages for free consumption of solid feed and water, and maintained at a temperature of 22 ± 1 ℃ and 60 ± 5% relative humidity. I was. The normal group (NOR) received 1.5 ml / kg of 0.9% saline for 14 days and the control group (CON: LPS-treated group) received 1.5 ml / kg of 0.9% saline for 14 days ( On the 15th day after saline) was administered daily, LPS (lipopolysaccharide) was prepared at a concentration of 5 mg / kg and administered intraperitoneally at a dose of 1.5 ml / kg.

The organic cherry tomato group and the organic droplet tomato + olive oil group were administered 1.5 ml / kg intraperitoneally at a concentration of 100 mg / kg for 14 days, and on the 15th day, a dose of 1.5 ml / kg LPS was administered intraperitoneally.

After administering and fasting LPS, 5 hours after the anesthesia with ether (ether) and dissected, the blood was collected and tested.

2. Liver Extraction and Blood Collection

After the end of the sample administration period, the experimental animals were opened under ether anesthesia, and then all four livers were removed, adsorbed with 0.9% physiological saline for washing, and then stored in a vial and stored in a -70 ° C deep freezer. Blood was collected using a 5 ml syringe from the heart and left at room temperature for 30 minutes, followed by centrifugation at 3000 rpm to use the upper serum portion.

3. Determination of Cholesterol, GOT and GPT in Serum

 Take 0.02 ml of each sample for sample, standard, and reagent blanks, add 3.0 ml of enzyme reagent, mix well, and leave at 37 ° C for 5 minutes, and then use a biochemical analyzer (Fuji) within 60 minutes. Absorbance at 500 nm was measured using a dri-chem.

4. Prostate cancer cell (PC 3) survival rate measurement (MTT assay)

1) Cell Culture

Human prostate cancer cells (PC3 cells) were purchased from Korea Cell Line Bank.

Cells were cultured in a 37 ° C., 5% CO 2 incubator using DMEM medium containing 1% FBS and 1% penicillin / streptomycin.

2) Cell viability measurement (MTT assay)

After dispensing PC3 cells (2 x 10 ⁴ ) in a 96-wellplate and stabilizing for 24 hours, the sample solution was diluted with PBS and filtered by membrane filter.

The treated supernatant was added to 20 μl of MTT (3- (4,5-dimethyl-2-thiazol) -2,5-diphenyl-2H-tetrazolim bromide, Sigma, USA) solution after 48 hours using ELISA microplate reader. Absorbance was measured at 540 nm.

Erectile dysfunction is a type of vascular disease, and more than 30% of patients with vascular disease have erectile dysfunction, which is one of sexual dysfunctions. Representative of vascular diseases include hyperlipidemia and liver disease. One of the diagnostic indicators of hyperlipidemia is cholesterol, and one of the diagnostic indicators of liver disease is GOT and GPT.

If you have prostate cancer, you will have erectile dysfunction.

If you explain the experiment

1) In order to see the effects on vascular disease, the case of using only organic tomatoes and olive oil was added to the organic tomatoes as shown in Table 1, 2, 3, 4. As a result, when olive oil was added to the organic tomato, the cholesterol, GOT and GPT levels were lowered.

2) Based on the above effects on vascular disease, in order to see the effects on prostate cancer, only the organic cherry tomatoes were used and the olive oil was added to the organic cherry tomatoes as shown in Table 5. As a result, the addition of olive oil to the organic cherry tomato was confirmed that the level of cancer cell survival rate is lowered.

3) Based on the above results that are effective for prostate cancer, three hundred seconds fermented stock solution (the material used in Patent Registration No. 10-0508539) known to enhance male reproductive function and snail chondroitin (patented to bring about improved liver function) The effects on prostate cancer were compared with the case of the drop tomato used with the addition of olive oil) and the case of the cherry tomatoes used with the addition of olive oil.

As a result, it was confirmed that the addition of three hundred seconds fermentation stock and snail chondroitin lowered the prostate cancer cell survival rate.

4) Olive oil is added to Saw Palmetto products, which are known to have good prostate cancer effects, based on the above 1) to 3), and secondly, the fermentation stock of 300 seconds and snail chondroitin are added. One case was compared with Table 7. As a result, it was confirmed that the cancer cell survival rate is almost the same.

In this way, it was possible to develop functional processed foods to enhance the prostate function using organic droplet tomato, which can replace the saw palmetto product, which is an expensive imported raw material.

cholesterol Experimental group NOR
(Normal group) CON (Control-LPS)
Administration group) organic
Cherry tomato Organic Cherry Tomato
+ Olive oil Cholesterol (mg / dl) 133 258 120 99

 LDL-cholesterol Experimental group NOR
(Normal group) CON
(Control group)
LPS
Administration group) organic
Cherry tomato organic
Cherry Tomato +
Olive oil LDL-sterol
(mg / dl) 27 180 39 18

 GOT Experimental group NOR
(Normal group) CON (Control)
LPS administration group) Organic Cherry Tomato Organic Cherry Tomato
+ Olive oil GOT (u / l) 62 192 165 64

 GPT Experimental group NOR
(Normal group) CON (Control)
LPS administration group) Organic Cherry Tomato Organic Cherry Tomato
+ Olive oil GPT (u / l) 39 93 65 57

MTT ASSAY is a method of measuring the number of living cells when a substance is administered to cultured cells. In the case of cancer cells, the lower the cell survival rate, the more cancer cells are killed. Meaning.

Prostate Cancer Cell (PC 3) Viability Measurement MTT ASSAY  Experimental group NOR Organic Cherry Tomato Organic Cherry Tomato
+ Olive oil Survival rate (%) 100 75 70

Prostate Cancer Cell (PC 3) Viability Measurement MTT ASSAY  Experimental group NOR
(Normal group) Organic Ultomato + Olive
oil Organic Cherry Tomato
+ Olive oil
+300 seconds fermented solution
+ Snail chondroitin
Survival rate (%) 100 70 65

Prostate Cancer Cell (PC 3) Viability Measurement MTT ASSAY Experimental group NOR
(Normal group) Organic Cherry Tomato
+ Olive oil
+300 seconds fermented solution
+ Snail chondroitin Saw palmetto Survival rate (%) 100 65 63

1 is a block diagram according to the method for producing cherry tomatoes processed food provided by the present invention

Claims (2)

After harvesting and washing organically grown cherry tomatoes, 100% by weight of naturally dried and crushed cherry tomatoes were mixed with 0.05% olive oil, and then fermented with three hundred seconds and brown sugar in a weight ratio of 49:51. 1% by weight of the fermented stock solution of 300 seconds and 0.1% by weight of snail chondroitin prepared by aging the snail and freeze-dried powder were aged with sodium acetate solution, Put the mixed solution into a dual-pressure steam machine, the concentrated liquid prepared by heating the concentrated solution prepared by heating for 1 hour under a pressure of 1.5kgf / ㎠ and 100 ~ 110 ℃ concentrated powder powder to enhance the prostate function, characterized in that . Harvesting and washing the organically grown cherry tomatoes and naturally drying them; Pulverizing the washed and dried cherry tomatoes with a grinder; After mixing 0.05% by weight of olive oil with 100% by weight of the pulverized drop tomato solution, 1% by weight of 300 ml of fermentation stock solution prepared by mixing and fermenting three hundred seconds and brown sugar in a weight ratio of 49:51, and freeze-dried snails Mixing the pulverized powder with sodium acetate solution to 0.1% by weight of snail chondroitin; Preparing a concentrated solution by adding a mixed solution to a sack and putting the mixed pressure into a dual pressure steamer for 1 hour under a pressure of 1.5 kgf / cm 2 and a temperature of 100 to 110 ° C. to remove dry matter; Cooling the concentrate to about 20 ° C. at room temperature; The method of claim 1, wherein the cooled concentrate is dried by using a freezing dryer to produce concentrated powder.

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