KR101094264B1 - Acinetobacter sp. SMIC-1, a carrier comprising the same and a method of reducing non-biodegradable materials using the same - Google Patents

Abstract

Acinetobacter sp. SMIC-1 ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P) strain, a carrier comprising the same, and a method for reducing the hardly decomposable substance using the same, according to one or more embodiments of the acinetobacter According to the genus SMIC-1 ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P), a carrier comprising the same, and a method for sequencing a non-degradable substance using the same, a method for efficiently determining a Can be reduced.

Acinetobacter, refractory substance, wastewater, carrier

Description

Acinetobacter sp. SCI-1, a carrier comprising the same and a method for reducing the hardly decomposable substance using the same {Acinetobacter sp. SMIC-1, a carrier comprising the same and a method of reducing non-biodegradable materials using the same}

One or more embodiments are directed to a strain of Acinetobacter sp. SMIC-1, a carrier comprising the same and a method for reducing hardly decomposable substances using the same.

Wastewater from the electronics industry, such as semiconductors and LCDs, is derived from developer liquids used in photolithography and exofoliation liquids used in etching. The main components of the wastewater include tetramethyl ammonium hydroxide, which is used as a developer, diethylene glycol monoethyl ether, and diethylene glycol monomethyl ether, which are used as outer membrane solution and cleaning agent. monomethyl ether), tetrahydrothiophene 1,1-dioxide, 1-methyl-2-pyrrolidinone, tetraethylene glycol and 1-amino-2-propanol and the like. If untreated chemicals remain in the effluent, there is a potential risk of long-term environmental exposure, especially since these materials are not biodegradable and are difficult to process in a general activated sludge process, so that semiconductors and LCDs can Appropriate treatment of chemicals used in manufacturing processes and the like is required.

The related art discloses a concentrated waste method in which waste water is concentrated and disposed of by an ion exchange resin column, ultrafiltration or reverse osmosis, or a supercritical treatment method in which the concentrated waste water is burned and removed. However, this method has a very complicated step and has the disadvantage of causing secondary pollution due to concentration or combustion.

Thus, even with the prior art, there is still a need to develop a method for treating economically and efficiently hardly decomposable materials in wastewater without causing further contamination.

One embodiment provides a strain of Acinetobacter sp. SMIC-1.

Another embodiment is to provide a method of reducing hardly decomposable substances in wastewater, comprising contacting the wastewater comprising hardly decomposable substances with a strain of Acinetobacter sp. SMIC-1.

Another embodiment is to provide a carrier to which the Acinetobacter sp. SMIC-1 strain is immobilized.

One embodiment is a genus of Acinetobacter which grows with at least one hardly decomposable substance selected from the group consisting of polyalkylene glycols, polyalkylene glycol monoalkyl ethers, thiophene derivatives and pyrrolidinone derivatives as carbon and energy sources. A strain SIC-1 ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P) is provided.

The term "non-biodegradable material" refers to a material that can be degraded by microorganisms, but it takes a long time to decompose because it is very slow. For example, physical or chemical methods are generally used to reduce or eliminate the hardly degradable materials in any sample.

The strain is resistant to the hardly decomposable substance, and may be grown as a carbon source and an energy source. The concentration of the hardly decomposable substance that the strain may be resistant to may be 50 ppb to 10,000 ppm, respectively. Therefore, the strain may be used to reduce the concentration of the hardly decomposable substance in the sample containing the high concentration of the hardly decomposable substance.

As the type of the hardly decomposable substance, for example, diethyleneglycol monoethyl ether, diethyleneglycol monomethyl ether, tetrahydrothiophene 1,1-dioxide (tetrahydrothiophene 1, 1-dioxide, tetraethyleneglycol and 1-methyl-2-pyrrolidinone may be included, but are not limited thereto.

One embodiment is to reduce the hardly degradable material in the wastewater, comprising contacting the wastewater comprising the hardly degradable material with a strain of genus Acinetobacter sp. SMIC-1 (Accession No. KCCM 10999P). It provides a method to make it.

The strain for reducing hardly decomposable substances in the wastewater may be pre-cultured to have a suitable population. Temperature, pH conditions for culturing the strain may be determined by those skilled in the art by modifying the culture conditions of known strains, the temperature may be 20-40 ℃, pH may be 4-9.

In addition, any method known to those skilled in the art can be used to culture the strain. For example, batch culture, fed-batch culture or continuous culture may be used, but is not limited thereto. When culturing the strain in a continuous culture, the strain is inoculated into a fermenter containing a nutrient medium in an appropriate amount and incubated batchwise to a predetermined density (for example, OD = 0.2) or more, and then one or more of the eggs The sample containing the decomposable substance (for example, semiconductor factory wastewater) may be cultured while flowing at a constant rate. Cultivation of the strain may be carried out in agitation or non-shell state, it may be made in aerobic conditions. In addition, it is apparent to those skilled in the art that the culture of the strain may further include a medium component for promoting the growth of the strain.

The strain may be immobilized on a carrier. The carrier may be made of a material selected from the group consisting of collagen, gelatin, agar, alginic acid, color quinone, cellulose, acetate, polyacrylamide, amide, polyurethane, polyvinyl alcohol and polyalkylene glycol. It is not limited to this. By immobilizing the strain on a carrier, it is possible to limit the movement of the strain so that the strain is located in an appropriate space.

The carrier may be in the form of activated sludge containing the strain.

The term “activated sludge” refers to a community of aerobic microorganisms that breed when abundantly pumping sewage or wastewater to supply oxygen, and the activated sludge may play a major role in the biological treatment of sewage or wastewater.

The carrier may further comprise kaolin, granite, barium sulfate or kaolin. By further including the substance in the carrier to control the specific gravity of the carrier, the carrier containing the strain may be placed in a liquid sample (eg, wastewater) to decompose the hardly decomposable substance in the liquid sample. In addition, when the carrier containing the strain does not contain the substance, it may be suspended on the liquid sample to decompose the hardly decomposable substance present on the liquid surface.

On the other hand, the wastewater may be any sample containing a hardly decomposable substance, and may be, for example, an undiluted wastewater containing the hardly decomposable substance or diluted wastewater. The wastewater may include, for example, one or more materials selected from the group consisting of a developer, a stripping solution, an outer membrane solution, and a cleaning solution, and the types of hardly decomposable materials that may be included in the wastewater are as described above.

The method for reducing hardly decomposable substances in the wastewater may use any wastewater treatment method known to those skilled in the art, for example, an activated sludge treatment process, an advanced oxygen process, or a biological filtration method. It may be performed by a biological treatment system such as an aerated filter, a trickling filter process, an oxidation pond, or a rotating biological contactor, but is not limited thereto. For example, when the activated sludge method is applied, the administration method, timing of administration, administration conditions, etc. of the strain can be arbitrarily selected by those skilled in the art.

One embodiment provides a carrier to which a strain of Acinetobacter sp. SMIC-1 (Accession No. KCCM 10999P) is immobilized.

The strain may be in the form of activated sludge, and the carrier including the strain may be hydrophilic. In addition, the concentration of the strain in the form of activated sludge to reduce hardly degradable substances in the wastewater may be 50 to 100,000 MLSS (mg / l).

The term "Mixed Liquor Suspended Solid" (MLSS) refers to an indicator indicating suspended solids in activated sludge or suspended solids in aeration tank mixture, and can be used as a very important factor in sewage and wastewater treatment facilities. In other words, the suspended solids of the mixed liquid in the aeration tank is expressed in mg / L, which means the concentration of activated sludge.

On the other hand, the carrier may be made of a material selected from the group consisting of collagen, gelatin, agar, alginic acid, color quinone, cellulose, acetate, polyacrylamide, amide, polyurethane, polyvinyl alcohol and polyalkylene glycol, The carrier may be poly (ethylene glycol) monoacrylate, poly (ethylene glycol) diacrylate, poly (ethylene glycol) monomethacrylate, poly (ethylene glycol) dimethacrylate, poly (ethylene glycol) divinyl ether, Poly (ethylene glycol) ethyl ether methacrylate, poly (ethylene glycol) methyl ether acrylate, poly (ethylene glycol) methyl ether methacrylate, poly (ethylene glycol) phenyl ether acrylate, poly (ethylene glycol) urethane monoacrylic Elate, poly (ethylene glycol) urethane diacrylate, poly (ethylene Recall) urethane methacrylate, poly (ethylene glycol) urethane dimethacrylate, poly (ethylene glycol) urethane triacrylate, poly (ethylene glycol) urethane trimethacrylate, poly (ethylene-co-propylene glycol) monometha Crates, poly (ethylene-co-propylene glycol) methyl ether methacrylate, poly (ethylene-co-propylene glycol) ethyl ether methacrylate, poly (ethylene-co-propylene glycol) butyl ether methacrylate, poly (Ethylene-co-propylene glycol) monoacrylate, poly (ethylene-co-propylene glycol) methyl ether acrylate, poly (ethylene-co-propylene glycol) ethyl ether acrylate and poly (ethylene-co-propylene glycol) butyl Polyalkyl formed by polymerizing at least one substance selected from the group consisting of ether acrylates Lenglycol-based polymer may be a polyalkylene glycol-based polymer of a three-dimensional network structure is formed cross-linking between the backbone (backbone).

The carrier may be prepared as described in Korean Patent No. 596,366 disclosed by the inventors, which is incorporated herein by reference.

According to at least one embodiment of the genus Acinetobacter sp. SMIC-1 (Accession No. KCCM 10999P) strain, a carrier comprising the same and a method for reducing the hardly decomposable substance using the same, It is possible to efficiently reduce the hardly decomposable substance in the containing sample.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example

Example  1: Isolation of Refractory Resistant Strains

In this embodiment, wastewater activated sludge was collected from a wastewater treatment plant of a semiconductor manufacturing plant in Korea, and a hardly decomposable resistant strain was isolated therefrom.

First, inoculate about 0.2 ml by continuously diluting activated sludge of a semiconductor plant wastewater treatment plant to a minimal solid medium (minimal agar plate) having a composition of Table 1 containing hardly decomposable substances, and incubating at 30 ° C. for 5 days to grow. Five possible strains were isolated purely.

division ingredient Concentration (g / ℓ) Basic saline medium K ₂ HPO ₄ 9.28 KH ₂ PO ₄ 1.81 NH ₄ Cl 0.5 (NH ₄ ) ₂ SO ₄ 0.5 Na ₂ SO ₄ 0.5 MgSO _{4 7} H ₂ O 0.1 Carbon source Diethylene glycol monoethyl ether 2 Diethylene glycol monomethyl ether 2 Tetrahydrothiophene 1,1-dioxide 2 Tetraethylene glycol 2 1-methyl-2-pyrrolidinone 2 1-amino-2-propanol 2 Solid badge Agar 20 pH 7

Example  2: screening strains with excellent resolution of hardly decomposable substances

In this example, the indigestible digestion strains purely isolated in Example 1 were inoculated in a minimal medium containing the indigestible substances having the composition of Table 2 and incubated at 30 ° C. and 180 rpm for 2 days.

division ingredient Concentration (g / ℓ) Basic saline medium K ₂ HPO ₄ 9.28 KH ₂ PO ₄ 1.81 NH ₄ Cl 0.5 (NH ₄ ) ₂ SO ₄ 0.5 Na ₂ SO ₄ 0.5 MgSO _{4 7} H ₂ O 0.1 Carbon source Diethylene glycol monoethyl ether
Diethylene glycol monomethyl ether
Tetrahydrothiophene 1,1-dioxide
Tetraethylene glycol
1-methyl-2-pyrrolidinone
1-amino-2-propanol 0.1 each pH 7

After incubation, diethyleneglycol monoethyl ether, diethyleneglycol monomethyl ether, tetrahydrothiophene 1,1-dioxide, 1-methyl-2- in medium using gas chromatography-mass spectrometry (GC-MS) The residual amount of pyrrolidinone was analyzed and the residual amount of tetraethylene glycol, 1-amino-2-propanol was analyzed using liquid chromatography-mass spectrometry (HPLC-MS).

First, for the GC-MS analysis, 100 ml of the culture medium filtered through a filter having a pore size of 0.2 μm was placed in a separatory funnel, and 30 g of NaCl was added thereto. Thereafter, ethyleneglycol butyl ether was added to 1 mg / L as an internal standard, 10 ml of methylene chloride was added, and mixed for 20 minutes. After mixing, the separatory funnel was left until the solution was completely separated, and the methylene chloride layer of the separatory funnel was carefully recovered and used for GC-MS analysis. GC-MS analysis conditions used are shown in Table 3.

parameter Condition column DB-WAXETR 30m × 0.32mm × 1 μm (FT) carrier He (1.0 mL / min) Oven temperature 10 ℃ / min 10 ℃ / min 80 ℃ → 180 ℃ 180 ℃ → 250 ℃ (2 minutes) Split ratio Undivided Injector temperature 250 ℃ Moving temperature 280 ℃ Selected ion groups group Start time (minutes) compound Selected ions, m / z One 4.5 Ethylene Glycol Butyl Ether (ISTD) 57 2 6.5 Diethylene glycol monoethyl ether
Diethylene glycol monomethyl ether 45 3 8.5 1-methyl-2-pyrrolidinone 99 4 13.3 Tetrahydrothiophene 1,1-dioxide 41

In addition, for HPLC-MS analysis, 10 ml of the culture solution filtered with a filter having a pore size of 0.2 μm was diluted 10-fold with ultrapure water without pretreatment, and then LXQ (Thermo), an Ion-Trap method, was used. 10 μl was directly injected into the connected HP 1200 HPLC (Agilent) for analysis. Rx-SIL (3 × 50 mm, 1.8 μm) was used as the analysis column, and the mobile phase was methanol / H ₂ O / formic acid = 80/20 / 0.05 (v / v / v), and the flow rate was 0.1 ml / min. It was. The interface connection between the HPLC and the mass spectrometer was in electrospray positive ionization mode, and tetraethyleneglycol (TEG) and 1-amino-2-propanol in the selected ion monitoring mode. Selective ions of (1-amino-2-propanol, APOL) were quantified at 195.0 and 76.0 m / z of [M + H] ⁺ , respectively. Source voltage 5 kV. Ionization conditions of gas flow 10 psi, capillary voltage 10 V and capillary temperature 300 ° C. were used.

As shown in Table 4, as a result of comparing the resolution of each hardly decomposable substance, it was confirmed that the hardly decomposable substance except for 1-amino-2-propanol was decomposed among the hardly decomposable substances used in the experiment. The decomposition efficiency of 1,1-dioxide was about 10%, and the decomposition efficiency of the remaining compounds was 77.9% or more. The strain having the highest resolution of the hardly degradable substance from the isolated strains was named SMIC-1, and was used in the following experiment.

ingredient Influent
(mg / ℓ) Runoff
(mg / ℓ) Decomposition efficiency
(%) Diethylene glycol monoethyl ether 100 &Lt; 0.1 99.9 Diethylene glycol monomethyl ether 100 <0.1 99.9 Tetrahydrothiophene 1,1-dioxide 100 90.1 9.9 1-methyl-2-pyrrolidinone 100 <0.1 99.9 Tetraethylene glycol 100 22.1 77.9 1-amino-2-propanol 100 100 0

Example  3: SMIC Identification of -1 Strains

In this example, the SMIC-1 strain was identified molecularly by investigating the biochemical properties of the SMIC-1 strain, analyzing the sequence of 16s rDNA, and comparing it with the sequence of known strains.

SMIC-1 strains were plated in a solid medium consisting of agar 15 g / L minimal medium, and the shape of colonies formed was observed, and the single colonies obtained therefrom were liquid cultured in minimal medium, followed by culture of SMIC-1 strains. The shape and color were observed under a microscope, and gram staining and catalase activity confirmation tests were performed. The results are shown in Table 5 below.

characteristic SMIC-1 Cell morphology Coccus Gram dyeing Gram voice Catalase activity - Colony form Round Colony color White Colony size 1 μm

In addition, in order to analyze the sequence of 16s rDNA of SMIC-1 strain, genomic DNA of SMIC-2 strain was used as a template, and primers 27F (SEQ ID NO: 2) and 1492R (SEQ ID NO: 3), which are universal primers, were primers. PCR was performed. The amplified PCR product, which is part of the 16s rDNA, was confirmed by its size through 0.8% agarose gel electrophoresis, and was then queried by Macrogen (Korea) for sequencing. Part of the results of analyzing the 16s rDNA sequence of the SMIC-1 strain is shown in SEQ ID NO: 1.

The homology of the 16s rDNA sequence of SEQ ID NO: 1 to the NCBI database (www.ncbi.nlm.nih.gov/blast.cgi) was found to be the genus Acinetobacter (eg, Acinetobacter baumannii AB900 (GenBank accession NO. NZ_ABXK00000000), Acinetobacter baumannii AB307-0294 (GenBank accession NO. NC_011595), and Acinetobacter baumannii AB0057 (GenBank accession NO.

Strains isolated purely on the basis of the physiological biochemical and molecular biological properties are diethylene glycol monoethyl ether, diethylene glycol monomethyl ether, tetraethylene glycol, tetrahydrothiophene 1,1-dioxide and 1-methyl-2- It has been confirmed that it is new to the genus Acinetobacter, which is resistant to pyrrolidinone and can grow using the hardly decomposable substance as a carbon source and an energy source. It was named as SMIC-1 ( Acinetobacter sp. SMIC-1) in the genus Acinetobacter and was deposited on April 9, 2009 at the Korea Microorganism Conservation Center (KCCM), an international depository organization under the Budapest Treaty (Accession No. KCCM 10999P).

Figure 1a shows a gas chromatography-mass spectrometer of 100 ppm of a hardly decomposable substance of diethylene glycol monoethyl ether, diethylene glycol monomethyl ether, tetrahydrothiophene 1,1-dioxide, 1-methyl-2-pyrrolidinone. It is the result analyzed by (GC-MS).

Figure 1b is acinetobacter sp. SMIC-1 ( Acinetobacter sp. SMIC-1) after incubation for 48 hours in a medium containing the non-degradable material, after analysis of the remaining degradable material in the strain culture by GC-MS shown It is a graph.

In Figure 2 (A) is a result of the tetraethylene glycol was recovered after stirring for 48 hours without culturing the strain and analyzed by liquid chromatography-mass spectrometry (HPLC-MS), (B) is SMIC-1 genus Acinetobacter ( Acinetobacter sp. SMIC-1) was inoculated in a minimal medium containing tetraethylene glycol and incubated for 48 hours, followed by analysis of tetraethylene glycol remaining in the culture solution.

In FIG. 3, (A) is a result of analyzing 1-methyl-2-pyrrolidinone after stirring for 48 hours without culturing the strain, and analyzing by liquid chromatography-mass spectrometry (HPLC-MS), and (B) is acine. Tobacter genus SMIC-1 ( Acinetobacter sp. SMIC-1) was inoculated in a minimal medium containing 1-methyl-2-pyrrolidinone and incubated for 48 hours, followed by analysis of 1-methyl-2-pyrrolidinone remaining in the culture solution.

<110> Samsung Engineering Co., LTD. <120> Acinetobacter sp. SMIC-1, a carrier comprises the same and a          method of reducing non-biodegradable materials using the same <160> 3 <170> KopatentIn 1.71 <210> 1 <211> 420 <212> DNA <213> Acinetobacter sp. SMIC-1 <400> 1 cgtttatccg ggatttnctg ggcgtaaagc gtgcgtaggc ggcttaatta agtcaaatgt 60 gaaatccccg agcttaactt gggaattgca ttcgatactg gttagctaga gtatgggaga 120 ggatggtana attccaggtg tagcggtaaa atgcgtaaag atctggagga ataccgatgg 180 caaggcaccc tcttgcccta aaactgacgc taatnacgaa acttggggag ccacaggatt 240 aaataccctg gtgtccttcc ctaaacgatg tcaccacccg ttgggtcttt tnaaccttag 300 gggcccctaa ccaaaaaaaa aaancgcttg gggatttgcc cgcaagacta aactcaaatt 360 gttttttgga gcccccaaac ancggtgggg ctgggtgttt ttttttttca acggaaaacc 420                                                                          420 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F universal primer for 16s rDNA sequencing <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R universal primer for 16s rDNA sequencing <400> 3 tacggytacc ttgttacgac tt 22  

Claims (17)

SMIC-1 genus Acinetobacter capable of growing as a carbon and energy source one or more hardly decomposable substances selected from the group consisting of polyalkylene glycols, polyalkylene glycol monoalkyl ethers, thiophene derivatives and pyrrolidinone derivatives ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P) strain. The method of claim 1, wherein the hardly decomposable substance comprises diethylene glycol monoethyl ether, diethylene glycol monomethyl ether, tetrahydrothiophene 1,1-dioxide, tetraethylene glycol and 1-methyl-2-pyrrolidinone And at least one substance selected from the group. Acinetobacter genus SMIC-1 ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P) A method for reducing hardly decomposable substances in wastewater, comprising contacting the strain with wastewater comprising hardly decomposable substances. 4. Acinetobacter according to claim 3, wherein the genus SMIC-1 ( Acinetobacter) sp. SMIC-1) (Accession No. KCCM 10999P) strain is inoculated and cultured in a medium containing the hardly decomposable material. The method of claim 4, wherein the culturing is carried out at 20-40 ° C. and pH 4-9. The method of claim 3, wherein the hardly degradable material is at a concentration of 10,000 ppm or less. The method of claim 3, wherein the strain is immobilized on a carrier. The method of claim 7, wherein the carrier is made of a material selected from the group consisting of collagen, gelatin, agar, alginic acid, color quinone, cellulose, acetate, polyacrylamide, amide, polyurethane, polyvinyl alcohol and polyalkylene glycol. How. 4. The method of claim 3, wherein the wastewater comprises one or more materials selected from the group consisting of developer, stripper, outer membrane and wash. The method of claim 3, wherein the hardly degradable material is at least one material selected from the group consisting of polyalkylene glycols, polyalkylene glycol monoalkyl ethers, thiophene derivatives and pyrrolidinone derivatives. The method of claim 3, wherein the hardly decomposable substance is composed of diethylene glycol monoethyl ether, diethylene glycol monomethyl ether, tetrahydrothiophene 1,1-dioxide, tetraethylene glycol and 1-methyl-2-pyrrolidinone. At least one substance selected from the group. Acinetobacter genus SMIC-1 ( Acinetobacter sp. SMIC-1) (Accession No. KCCM 10999P) A carrier to which the strain is immobilized. The carrier of claim 12, wherein the strain is in the form of activated sludge. The carrier of claim 12, wherein the strain has a concentration of 50 to 100,000 MLSS (mg / L). The carrier of claim 12, wherein the carrier is hydrophilic. The method of claim 12, wherein the carrier is made of a material selected from the group consisting of collagen, gelatin, agar, alginic acid, color quinone, cellulose, acetate, polyacrylamide, amide, polyurethane, polyvinyl alcohol and polyalkylene glycol. Carrier. The method of claim 12, wherein the carrier is poly (ethylene glycol) monoacrylate, poly (ethylene glycol) diacrylate, poly (ethylene glycol) monomethacrylate, poly (ethylene glycol) dimethacrylate, poly (ethylene Glycol) divinyl ether, poly (ethylene glycol) ethyl ether methacrylate, poly (ethylene glycol) methyl ether acrylate, poly (ethylene glycol) methyl ether methacrylate, poly (ethylene glycol) phenyl ether acrylate, poly ( Ethylene glycol) urethane monoacrylate, poly (ethylene glycol) urethane diacrylate, poly (ethylene glycol) urethane methacrylate, poly (ethylene glycol) urethane dimethacrylate, poly (ethylene glycol) urethane triacrylate, poly (Ethylene glycol) urethane trimethacrylate, poly (ethylene co-propylene glyce ) Monomethacrylate, poly (ethylene-co-propylene glycol) methyl ether methacrylate, poly (ethylene-co-propylene glycol) ethyl ether methacrylate, poly (ethylene-co-propylene glycol) butyl ether methacrylate , Poly (ethylene-co-propylene glycol) monoacrylate, poly (ethylene-co-propylene glycol) methyl ether acrylate, poly (ethylene-co-propylene glycol) ethyl ether acrylate and poly (ethylene-co-propylene glycol Polyalkylene glycol-based polymer having a three-dimensional network structure crosslinked between the backbone of the polyalkylene glycol-based polymer formed by polymerization of at least one material selected from the group consisting of butyl ether acrylate Carrier.

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