KR101093531B1 - Kimch Mature Method for ???? producing - Google Patents

Abstract

The present invention relates to a kimchi ripening method for producing a large amount of gamma (amino-aminobutyric acid). In the present invention, lactic acid bacteria are grown from kimchi containing glutamic acid, and matured for 23-44 hours at a temperature of 13-16 ° C. to convert glutamic acid to GABA and increase the content of GABA using lactic acid bacteria.

Kimchi, GABA, Ripening

Description

Kimchi Mature Method for Baa Baa

The present invention relates to a method of ripening kimchi, and more particularly, to a kimchi ripening method of producing a large amount of GABA, a neurotransmitter, by aging kimchi at a constant temperature for a certain time.

Kimchi is fermented at low temperature to ensure the preservation and maturity of the product after mixing red pepper, garlic, sake, green onion and radish with pickled cabbage, which is the main raw material, and is the representative traditional fermented food unique to Korea.

In recent years, the nutritional value of kimchi is being re-evaluated, and it is necessary to develop kimchi with physiological functions at a new point of time in order to expand the market, not to expand demand for kimchi.

GABA, which can add physiological function to kimchi, is a kind of amino acid contained in the nervous system and blood in humans, and most of it is present in bone marrow, which increases brain function by increasing brain function by increasing neurotransmitter acetylcholine. It is known to be effective in improving brain metabolism, mental stability and stroke. In addition, hypertension prevention effect, diuretic effect, liver function improvement effect, obesity prevention action, alcohol metabolism promoting action, and the like are known, especially registered as a drug for stroke treatment.

The GABA is known to be widely distributed in various vegetables, fruits, rice, soybeans, etc., but its content is low, so it is difficult to exhibit physiological action. In addition, the existing GABA is a chemical synthesis product is mostly concerned about side effects such as anorexia, constipation, diarrhea.

Recently, when manufacturing kimchi, glutamic acid is supplied using egg yolk protein, and a method of converting a part of glutamic acid into gamma -aminobutyric acid (GABA) using lactic acid bacteria of kimchi is disclosed. However, in order to supply glutamic acid, fermented protein fermented using egg yolk protein, soy protein, and the United States (米 따) is prepared separately, and when the kimchi is added, it is not only cumbersome to prepare kimchi. There is a problem that less GABA is generated.

Accordingly, the present invention is to provide a kimchi ripening method of producing a large amount of GABA by aging kimchi containing glutamic acid only under certain kimchi ripening conditions.

To this end, the kimchi ripening method according to the present invention is to propagate lactic acid bacteria from kimchi containing glutamic acid, and convert the glutamic acid to GABA using lactic acid bacteria to increase the content of GABA 23-44 hours at a temperature of 13-16 ℃ To mature.

At this time, in addition to glutamic acid, a carbon source is further added to produce GABA. The glutamic acid to be added is derived from audi or seafood and the carbon source is derived from wheat gluten or corn gluten.

In addition, the pH of kimchi aged for 23-44 hours at a temperature of 13-16 ° C is 4.7-5.2, and the acidity is 0.57-0.59.

Here, lactic acid bacteria that can convert glutamic acid to GABA are lactic acid bacteria that are produced during kimchi ripening, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus plantarum, Leuconostoc Lactis, Lactococcus At least one may be selected from Leuconostoc mesenteroides, Leuconostoc dextranicum, Enterococcus casseliflavus and Streptococcus thermophilus.

As such, the kimchi ripening method of the present invention can produce a large amount of GABA that was limited by natural intake by aging kimchi containing glutamic acid at a temperature of 13-16 ℃ for 23-44 hours.

In addition, the kimchi of the present invention can be expected to have functionalities such as blood pressure drop, learning ability enhancement, and mental stability by GABA generated in large quantities.

In addition, the present invention does not cause side effects such as anorexia, constipation and diarrhea compared to when ingesting GABA formed as a chemical synthetic product by ingesting naturally occurring GABA in kimchi.

The present invention maintains optimum kimchi ripening conditions so that a large amount of GABA (γ-aminobutyric acid) can be produced by aging kimchi.

Hereinafter, a method of ripening kimchi for producing GABA of the present invention will be described in detail.

In the present invention, when producing kimchi by adding an additive such as glutamic acid to prepare kimchi and then ripening it under certain conditions, lactic acid bacteria can be proliferated and glutamic acid is converted into GABA by lactic acid bacteria to produce a large amount of GABA.

At this time, the kimchi ripening conditions for producing lactic acid bacteria and GABA in a large amount by aging kimchi is maintained at 13-16 ℃ for 23-44 hours, preferably kimchi at 15 ℃ for 36-40 hours. If the temperature is maintained below 13 ° C or above 16 ° C, the amount of GABA produced may decrease. In addition, if kimchi is less than 23 hours of fermentation, kimchi may not be fully matured, resulting in less lactic acid bacteria, which may reduce GABA production. If kimchi is aged for more than 44 hours, it may be used as a food for microorganisms. The production can be reduced.

The pH of this aged kimchi is 3.9-4.4 and the acidity (lactic acid concentration) is 0.47-0.53, and the taste of kimchi is good under these conditions.

At this time, the lactic acid bacteria that can be obtained by ripening kimchi Lactobacillus brevis, Lactobacillus platarum (Lactobacillus plantarum), Leuconostoc Lactis, Lactococcus mesenteroides (Leuconostoc mesenteroides) , Lactococcus dextranicum, Enterococcus casseliflavus and Streptococcus thermophilus, and one of the lactic acid bacteria is added to GABA with glutamic acid Can be converted.

Glutamic acid, which is added when preparing kimchi to produce GABA, can convert glutamic acid to GABA using lactic acid bacteria as a precursor of GABA, and its content is 2-3 parts by weight based on the total kimchi composition. The amount of GABA produced is large when the content of glutamic acid is 2-3 parts by weight based on the total kimchi composition. Materials that can provide such glutamic acid include MSG and Audi, as well as anchovies, anchovies, katsuo and flying fish, so any one selected from the above can be used in the production of kimchi to provide glutamic acid. However, the substance providing glutamic acid is not limited thereto.

In addition, additives added to produce GABA include NaCl and a carbon source. NaCl is generally used when salting cabbage and preparing kimchi, so no additional addition is required. In addition, it is preferable to use wheat gluten or corn gluten in terms of amino acid composition and flavor, and the amount of GABA produced is 0.5-1 parts by weight based on the total kimchi composition.

1 is a graph showing the content of GABA according to the aging time of the present invention.

Referring to Figure 1, it shows the content of GABA produced according to the ripening time while maintaining kimchi at 15 ℃ using a kimchi refrigerator, etc., the content of GABA per 100g kimchi is the highest when the ripening time is 36-40 hours .

Hereinafter, preferred examples are provided to aid the understanding of the present invention, but the following examples are merely for exemplifying the present invention, and it will be apparent to those skilled in the art that various changes and modifications can be made within the scope and spirit of the present invention. It is natural that such variations and modifications fall within the scope of the appended claims.

Example  1-4.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. 30 kg of pickled radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of shrimp sauce, 1.1 kg of wheat gluten, 2.1 kg of Katsuo were added, and a little salt and garlic were added to make kimchi. The kimchi thus prepared is put into a kimchi refrigerator to ripen. At this time, the temperature of the kimchi refrigerator is maintained at 15 ° C. for aging for 23, 36, 40 and 44 hours.

Example  5.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. 30 kg of radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of shrimp sauce, 1.1 kg of wheat gluten and 2.1 kg of mulberry were added, and a little salt and garlic were added to make kimchi. The kimchi thus prepared is put into a kimchi refrigerator to ripen. At this time, the temperature of the kimchi refrigerator is maintained at 15 ° C. and aged for 40 hours.

Comparative example  One.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. 30 kg of radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of salted shrimp, 1.1 kg of wheat gluten, and 3.5 kg of Katsuo were added, and a little salt and garlic were added to make kimchi. The kimchi thus prepared is put into a kimchi refrigerator to ripen. At this time, the temperature of the kimchi refrigerator is maintained at 15 ° C. and aged for 40 hours.

Comparative example  2.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. 30 kg of radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of salted shrimp, 1.1 kg of wheat gluten, 2.1 kg of Katsuo were added, and salt, garlic, etc. were added to make kimchi. The kimchi thus prepared is put into a kimchi refrigerator to ripen. At this time, the temperature of the kimchi refrigerator is maintained at 15 ° C. and aged for 60 hours.

Comparative example  3.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. 30 kg of radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of salted shrimp, 1.1 kg of wheat gluten, 2.1 kg of Katsuo were added, and salt, garlic, etc. were added to make kimchi. The kimchi thus prepared is put into a kimchi refrigerator for aging. At this time, the temperature of the kimchi refrigerator is maintained at 10 ° C. and aged for 40 hours.

Comparative example  4.

After 40 kg of Chinese cabbage, 1.2 kg of salt was evenly sprinkled and left for about 8 hours. Kimchi genus was made by adding 30 kg of radish, 1 kg of green onion, 0.4 kg of red pepper powder, 0.15 kg of sugar, 0.45 kg of shrimp sauce, some salt, garlic, fermented protein, and lactic acid bacteria culture. The kimchi thus prepared is put into a kimchi refrigerator for aging. At this time, the temperature of the kimchi refrigerator is maintained at 10 ° C. for aging for 48 hours.

At this time, the fermented protein was obtained by adding 0.8 kg of skim yolk protein, 0.2 kg of soybean embryo protein, 1 kg of water, 0.5 kg of red pepper powder, 0.2 kg of finely chopped garlic, and 0.5 kg of U.S. . In addition, the lactic acid bacteria culture medium was pre-cultivated at 30 ° C. for 1-2 days using lactic acid bacteria separation medium containing kimchi broth.

Test Example  One. GABA  Content measurement

In order to determine the content of GABA produced per 100 g of kimchi, an enzyme analysis method was used as a quantitative analysis of GABA. GABA transaminase converts GABA and alpha-ketoglutarate to glutamate and succinic semialdehyde, and the resulting succinic semialdehyde and NADP + are succinic semialdehyde dehydrogenases. Succinic and NADPH are produced by succinic semialdehyde dehydrogenase. The enzyme GABA transaminase, succinic acid semialdehyde dehydrogenase was added, and the necessary substrates alpha ketoglutamic acid and NADP + were added to determine the amount of GABA by the amount of NADPH generated after the enzymatic reaction.

Table 1 below is a table showing the content of GABA per 100 g of aged kimchi of Example 1-5 and Comparative Example 1-4.

Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 GABA content
(mg / 100g) 14.64 16.16 19.9 14.36 20.2 10.3 9.83 8.45 8.12

Looking at Table 1, the content of GABA was excellent when the kimchi was aged for 23-44 hours at 15 ℃ (Fig. 1). In addition, it was confirmed that the content of GABA is low at high glutamic acid content, long aging time, and low temperature as compared with Example 1-4. In addition, the fermented protein and lactic acid bacteria fermentation broth were prepared separately and then aged to prepare kimchi, which was not higher than the GABA content at the time of ripening under certain conditions.

In addition, the content of GABA did not change even after 50 days of storage at 4 ℃ after kimchi ripened.

Test Example  2. Sensory test

The kimchi prepared in Example 5 and Comparative Examples 1-4 were subjected to sensory tests on 50 people. The test result was 5 points out of perfection scale.

Table 2 below is a table evaluating the taste, appearance and smell of kimchi prepared in Example 5 and Comparative Examples 1-4.

Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 A comprehensive taste 4.0 3.9 4.1 4.0 4.4 3.8 3.6 3.5 3.6 Exterior 3.9 3.8 3.8 3.7 3.8 3.7 3.3 3.8 3.8 smell 3.5 3.4 3.5 3.4 3.9 3.2 2.8 3.3 3.5

Looking at Table 2, kimchi of Comparative Example 2 was low in appearance because the color of the cabbage is dark discoloration.

Kimchi of Example 1-5 has a synergistic effect on the taste while the amount of GABA is maintained at a certain level, especially the taste of Example 5 using the Audi is excellent.

In addition, Example 5 using the Audi is excellent in the smell compared to Example 1-4 and Comparative Example 1-4 by the use of the audio.

1 is a graph showing the content of GABA according to the aging time of the present invention.

Claims (5)

delete delete Kimchi containing glutamic acid of audi aged for 23-44 hours at a temperature of 13-16 ℃, the lactic acid bacteria were proliferated and at the same time the glutamic acid is converted to GABA by the lactic acid bacteria. The method of claim 3, The kimchi ripening method of kimchi further comprises a carbon source of wheat gluten or corn gluten. The method of claim 3, The aged kimchi pH 3.9-4.4, acidity 0.47-0.53 kimchi ripening method.

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