KR101093202B1 - Photoluminescent Peptide Nanostructure and Method for Preparing Thereof - Google Patents

Abstract

The present invention relates to a light emitting peptide nanostructure and a method of manufacturing the same, and more particularly, to form a nanostructure by self-assembling the peptide to act as a template of lanthanum-based ion dispersion and to improve the luminous power of the lanthanum-based ion The present invention relates to a method for enabling easy synthesis of nanostructures in which light emitters are uniformly dispersed through simple mixing of a light emitting peptide nanostructure and each precursor solution, which serve as a photosensitizer. The light emitting peptide nanostructures according to the present invention not only have excellent luminous power, but also have various luminous colors in the visible light region, and thus can be applied to various fields such as bio-imaging and biosensors.

Peptides, Luminescence, Nanomaterials, Lanthanides, Photoresists

Description

Photoluminescent Peptide Nanostructure and Method for Preparing Thereof

The present invention relates to a light-emitting peptide nanostructure prepared using the self-assembly properties of the peptide and a method for manufacturing the same.

Biomimetic processing, which has been in the limelight as a technology to replace conventional expensive nanolithography using photolithography or electron beam lithography, is based on self-assembly of molecules. Self-assembly is the original technology of nanotechnology, which artificially manipulates the pushing or pulling forces between molecules to spontaneously form reproducible nanostructures. It is a field that is highly likely to be technologically converged and developed in the fields of electronic materials and new materials including biochips and nanotubes, and manufacturing nanostructures required for the manufacture of highly integrated semiconductors. In particular, peptide-based self-assembled materials are expected to develop new nanomaterials, especially nanobiotechnology, using them because of their superior cognitive abilities, specific chemical properties, and biological and environmental friendliness. Peptide-based self-assembled nanomaterials have thermal / chemical stability and excellent mechanical properties, but there is a lack of research on practical application to optical, electronic and biomaterials (M. Reches & E. Gazit, Sciene). , 300: 625, 2003; X. Gao & H. Matsui, Adv . Mater . , 17: 2037, 2005; I. Hamley, Angew . Chem . Int . Ed . , 46: 8128, 2007).

On the other hand, a europium (Eu ^{3 +),} terbium (Tb ^{3 +)} lanthanide metal ions (with a wavelength difference between the excitation light [excitation beam] and the light emitting beam (emission beam)) light emission, a large Stoke shift in the visible light region, such as Since it has advantages, the development of a new light emitting material using the same has attracted much attention. However, lanthanum-based metal ions have a disadvantage in that their light absorption area is very small and their luminescence intensity is very weak. Therefore, in order to overcome the lanthanide-based metal ions, the organic photosensitive molecules and lanthanum ions are chemically bonded or trapped in nano-sized cavities. And researches on improving luminous efficiency have been widely conducted in recent years (Y. Wada et. al ., Angew . Chem . Int . Ed ., 45: 1925, 2006; JCG Bunzli, Acc . Chem. Res . , 39:53, 2006; D. Zhao et al ., Adv . Mater ., 19: 3473, 2007). However, most of the light-emitting materials using lanthanum ions developed to date have not only a disadvantage of requiring complicated processes such as ion exchange and high temperature heat treatment, but also lack of biocompatibility of the material itself, resulting in bio-imaging and bio-sensing. It is difficult to use in various fields such as.

Accordingly, the present inventors have made efforts to develop a light emitting material using a lanthanum-based ion having a new structure that can be used in various fields such as bio-imaging and bio-sensing by a simple process. It was confirmed that the peptide nanostructure can be prepared, and completed the present invention.

An object of the present invention is to provide a method for manufacturing a light emitting material that can be utilized in various fields such as bio imaging and bio sensing in a simple process.

Another object of the present invention is to provide a light emitting nanostructure having a novel structure excellent in light emission characteristics using lanthanum-based ions.

In order to achieve the above object, the present invention provides a method for producing a light-emitting peptide nanostructure using self-assembly of the peptide, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions or salicylic acid is dissolved; And

(c) mixing the peptide solution prepared in step (a) with the solution prepared in step (b) to prepare a light-emitting peptide nanostructure in which a conjugate of the peptide and the lanthanide-based ion or salicylic acid is self-assembled .

The present invention also provides a light-emitting peptide nanostructure in which the conjugate of the peptide prepared by the above method and the lanthanide-based ion or salicylic acid is self-assembled.

The present invention also provides a method for preparing a light-emitting peptide nanostructure using self-assembly of a peptide, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which a photosensitive agent is dissolved; And

(c) a light-emitting peptide nanostructure in which the peptide solution prepared in step (a) and the lanthanum-based ion solution and the photosensitizer solution prepared in step (b) are mixed to self-assemble a peptide, a lanthanum-based ion and a photosensitizer solution. Manufacturing step.

The present invention also provides a light-emitting peptide nanostructure in which a combination of peptide, lanthanum-based ions and photosensitizer prepared by the above method is self-assembled.

The present invention also provides a method for preparing a light emitting peptide nanostructure having a desired emission color in the visible region, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which salicylic acid is dissolved; And

(c) mixing the peptide solution prepared in step (a) with the solution of step (b) and self-assembling, wherein the amount of each solution of step (b) to be mixed is adjusted to the desired emission color in the visible region Preparing a light emitting peptide nanostructure having a.

The present invention also provides a light emitting peptide nanostructure having a desired light emission color in the visible light region produced by the method.

The present invention also provides a sensor for pH measurement comprising the light-emitting peptide nanostructures.

The present invention also provides a method for measuring pH, characterized in that using the light-emitting peptide nanostructures.

The present invention has the effect of providing a light-emitting peptide nanostructure prepared using the self-assembly properties of the peptide and its preparation. The method for preparing a light emitting peptide nanostructure according to the present invention enables easy synthesis of nanostructures in which light emitters are uniformly dispersed through simple mixing of a solution, and the peptide nanostructures thus formed serve as a template for dispersion of lanthanide ions. In addition, it serves as a photosensitive agent to improve the luminous power of the lanthanum-based ions, thereby providing a nanostructure with improved luminous power. In this case, when another photosensitive agent having a resonant energy level is added in the middle of the energy level between the peptide and the lanthanum-based ion, the peptide and the photosensitive agent may generate synergistic effects, thereby obtaining a greatly improved luminescent effect. In addition, the preparation method according to the present invention is to mix the solution of the peptide dissolved with the three primary light emitting precursors together to adjust the amount of each solution, thereby allowing the synthesis of peptide nanostructures that emit light corresponding to all areas of visible light do. Therefore, the light emitting peptide nanostructure according to the present invention is very useful because it can be applied to various fields such as bio-imaging and biosensor.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

Definitions of main terms used in the detailed description of the present invention are as follows.

As used herein, the term "self-assembly" is a concept encompassing the assembly induced by non-covalent bonds (hydrogen bonds, ionic bonds, van der Waals bonds, hydrophobic bonds, electrostatic bonds, etc.). The peptide was used as.

As used herein, the term "photosensitive agent" refers to a material that increases the property of causing chemical change by the action of radiation, such as light, X-rays, gamma rays, and neutron rays, and includes organic photosensitive molecules. It means a substance that improves force.

The present invention is based on the discovery that the peptide nanostructure not only can serve as a framework in which lanthanide-based ions or salicylic acid, which are emitters, can be evenly dispersed, but also act as a photosensitizer to improve their luminous efficiency. In one aspect, the present invention relates to a method for preparing a light-emitting peptide nanostructure using self-assembly of a peptide, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions or salicylic acid is dissolved; And

(c) mixing the peptide solution prepared in step (a) with the solution prepared in step (b) to prepare a light-emitting peptide nanostructure in which a conjugate of the peptide and the lanthanum-based ion or salicylic acid is self-assembled; .

The light emitting peptide nanostructure according to the present invention is formed by inducing self-assembly of a peptide by simple mixing with a solution in which a lanthanide ion is dissolved, a peptide solution in which a peptide inducing self-assembly is dissolved using an organic solvent. In this case, the lanthanum-based ions, which are light emitters, are distributed in the nanostructures in the self-assembly process. The peptide nanostructures serve as templates in the dispersion of the light-emitting bodies, and at the same time, increase the luminous efficiency of the lanthanum-based ions.

In the present invention, as a peptide capable of causing self-assembly, hydrophobic dipeptide or a chemical derivative thereof capable of forming a one-dimensional nanostructure can be used, which is a compound of Table 1 and ( pentafluoro-phenylalanine)-(pentafluoro-phenylalanine) dipeptide, (iodo-phenylalanine)-(iodo-phenylalanine) dipeptide, (4-phenyl phenylalanine)-(4-phenyl phenylalanine) dipeptide, (p-nitro-phenylalanine)-(p -nitro-phenylalanine) dipeptide, phenylalanine-phenylalanine dipeptide, fluorenylmethoxycarbonyl (FMOC) -phenylalanine-phenylalanine dipeptide, phenylalanine-phenylalanine-cystein tripeptide, and the like.

TABLE 1

Chemical formula X (optional from list) Y (optional from list) NH ₂ -XY-COOH Alanine (Ala)
Valine (Val)
Isoleucine (Ile)
Leucine (Leu)
Phenylalanine (Phe)
Tryptophan (Trp) Alanine (Ala)
Valine (Val)
Isoleucine (Ile)
Leucine (Leu)
Phenylalanine (Phe)
Tryptophan (Trp) NH ₂ -XY-NH ₂ Phenylalanine (Phe) Phenylalanine (Phe) FMOC-X-Y-COOH Glycine (Gly)
Alanine (Ala)
Leucine (Leu)
Phenylalanine (Phe)
Lysine (Lys) Glycine (Gly)
Alanine (Ala)
Leucine (Leu)
Phenylalanine (Phe)
Lysine (Lys)

Since the peptide used in the preparation of the present invention has a small molecular weight and a small size, and the strength of intermolecular interactions is large, unlike the chemical self-assembly system that is carried out at a high temperature for several days, self-assembly can be made in a short time. The one-dimensional nanostructures manufactured by the present invention can form a hierarchical structure having a high degree of complexity while having complex functionalities.

In this case, the peptide is dissolved in an organic solvent. Preferably, the organic solvent is capable of dissolving a peptide that causes self-assembly. 1,1,1,3,3,3-hexafluoro-2-propanol A highly volatile solvent that can completely dissolve the peptide in monomer form, such as (1,1,1,3,3,3-hexafluoro-2-propanol), trifluoroacetic acid, etc. Use

In the present invention, lanthanum-based ions such as terbium, europium, erbium, neodymium, praseodymium, and the like may be used as the light emitter. Europium, terbium and mixtures thereof are used. Their chemical structures are shown in Figure 1 (a). On the other hand, salicylic acid plays a role of improving the luminous power as an organic photosensitizer, but may itself also serve as a blue light emitter. Thus, in the present invention, salicylic acid was used as the light emitter together with the lanthanum ions. The lanthanum-based ions or salicylic acid may be dissolved in an alcohol such as methanol or water as a solvent and mixed with the peptide solution in the form of a solution.

On the other hand, when adding a solution in which the photosensitizer is dissolved in the mixing step of the peptide solution and the lanthanum-based ion solution, the luminous effect of the lanthanum-based ions can be maximized to produce a high-efficiency light-emitting peptide nanostructure. Thus, in another aspect, the present invention relates to a method for preparing a light-emitting peptide nanostructure using self-assembly of a peptide, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which a photosensitive agent is dissolved; And

(c) a light-emitting peptide nanostructure in which the peptide solution prepared in step (a) and the lanthanum-based ion solution and the photosensitizer solution prepared in step (b) are mixed to self-assemble a peptide, a lanthanum-based ion and a photosensitizer solution. Manufacturing step.

At this time, the photosensitizer is selected from the group consisting of 4-acetylbiphenyl (4-acetylbiphenyl), benzophenone, 1,10-phenanthroline (1,10-Phenanthroline), derivatives thereof and mixtures thereof In the step (b), the solvent for dissolving the lanthanum-based ions and the photosensitizer may be alcohol or water such as methanol.

In one embodiment of the present invention, it was confirmed that the presence of the peptide nanostructure greatly improves the light absorption properties and light emission characteristics of the light emitting material such as lanthanum-based ions, in particular red (peptide + 1, 10-phenanthroline + europium), It was confirmed that light emitting peptide nanostructures of three primary colors of green (peptide + 1,10-phenanthroline + terbium) and blue (peptide + salicylic acid) can be prepared.

In addition, by mixing one or more light emitters (lanthanum-based ions, salicylic acid) to the nanostructures can be produced peptide nanostructures of a new color different from the color of the existing light emitter. That is, in the mixing step of the solution causing the self-assembly of the peptide, the peptide solution and one or more light emitting solutions are mixed together, and the desired color may be obtained by adjusting the mixing ratio of the light emitting solution. Thus, in another aspect, the present invention relates to a method for preparing a light emitting peptide nanostructure having a desired emission color in the visible region, comprising the following steps:

(a) dissolving the peptide in an organic solvent;

(b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which salicylic acid is dissolved; And

(c) mixing the peptide solution prepared in step (a) with the solution of step (b) and self-assembling, wherein the amount of each solution of step (b) to be mixed is adjusted to the desired emission color in the visible region Preparing a light emitting peptide nanostructure having a.

At this time, the solvent for dissolving one or more light emitters (lanthanum-based ions, salicylic acid) in the step (b) may use alcohol or water, such as methanol, and further in the step (c) to increase the luminous efficiency It may be characterized by mixing together. In this case, as the photosensitizer, one selected from the group consisting of 4-acetylbiphenyl, benzophenone, 1,10-phenanthroline, derivatives thereof, and mixtures thereof may be used.

In one embodiment of the present invention, as a result of mixing a mixture of europium, lanthanum ions, and terbium and salicylic acid, respectively, by varying the mixing ratio to the peptide solution, it was confirmed that peptide nanostructures having various emission colors could be synthesized. . The mixing ratio of each component and the color of the resulting peptide nanostructure can be confirmed through the table shown in FIG. 6 and the Commission Internationale de L'Eclariage (CIE) colorimeter.

On the other hand, in another embodiment of the present invention, it was confirmed that the light-emitting peptide nanostructure according to the present invention changes the emission color according to the pH, it can be usefully used as an environmental sensor, such as a sensor for measuring pH. Accordingly, the present invention provides a sensor for measuring pH including the light-emitting peptide nanostructures and a method for measuring pH using the same.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

In particular, in the following examples, only diphenylalanine or Fmoc-diphenylalanine was used as a precursor for synthesizing peptide nanostructures. However, if the peptides can induce self-assembly, the method according to the present invention can be applied. It is obvious to those skilled in the art.

Example  1: luminous Diphenylalanine Peptide  Preparation of Nanostructures

1-1. radiation Diphenylalanine Peptide  Preparation of Nanostructures

Figure 1a is a chemical structural formula of the precursor used in the synthesis of light-emitting peptide nanostructures according to the present invention. First, 100 mg of diphenylalanine (FF) was dissolved in 1 ml of 1,1,1,3,3,3-hexafluoro-2-propanol to completely dissolve the peptide in monomer form to prepare a peptide solution. In addition, a solution was prepared in which organic photosensitive molecules such as 4-acetylbiphenyl, 1,10-phenanthroline and salicylic acid, and lanthanide salts such as europium chloride and terbium chloride were dissolved in methanol at a concentration of 10 mM, respectively.

Then, the prepared peptide solution, photosensitive molecule solution and lanthanum ion solution were diluted 1/10 in methanol, and stirred for about 5 minutes to synthesize light-emitting peptide nanostructures according to the present invention. The luminescence properties of the synthesized peptide nanostructures were analyzed using a fluorescence spectrometer (Shimadzu, RF-5301PC) and a fluorescence microscope (Nikon, Eclipse 80i).

As a result, as shown in the photomicrograph of Figure 1b-c, it was possible to observe the change in the luminescence properties according to the composition of the peptide, organophoton molecules and lanthanum ion. That is, when 1,10-phenanthroline (PHEN), salicylic acid (SA) and the like are bound to the peptide nanostructure with lanthanide-based ions, it was confirmed that the red, green, and blue emission behaviors were shown.

The organophotosensitive molecules and lanthanum ions are determined to bind to the peptide nanostructures through hydrophobic and electrostatic interactions, and energy dispersive X-ray spectroscopy (EX- 250, Horiba, Japan), and as a result, it was confirmed that lanthanum ion was well overlapped with the peptide nanostructures.

Meanwhile, as a result of analyzing the X-ray diffraction (Rigaku, D / MAX-IIIC) and transmission electron microscope (Philips, Tecnai F20) of the light-emitting peptide nanostructures, as shown in Figure 3, the lanthanide-based ions and organic photosensitive molecules It was confirmed that the presence or absence does not significantly affect the crystal structure and morphology of the peptide nanostructures formed through self-assembly.

1-2. Analysis of the Effect of Peptide Nanostructures on Lanthanum Ion Luminescence

In order to analyze the luminescence properties when lanthanide and organophotosensitive molecules bind to the peptide nanostructures, i) when each precursor is independently present, ii) only salicylic acid organophotosensitive molecules (SA) are peptide nanostructures (FF). Iii) solution-phase light emission when organophotosensitive molecules (SA) and terbium ions (Tb) are bound, and iii) organophotosensitive molecules (SA) and terbium ions (Tb) are bound to peptide nanostructures (FF). The properties were measured.

As a result, as shown in Figure 4, when the organic photosensitive molecules and lanthanum ions are coupled to the peptide nanostructures, it was confirmed that the absorption and emission characteristics are greatly improved.

On the other hand, as a result of closely examining the emission spectrum according to the composition, it was confirmed that the luminous efficiency of the lanthanum ion is maximized by the effect of the peptide nanostructure (FF). That is, it was confirmed that the peptide nanostructure not only serves as a host matrix to fix the organic photosensitive molecules and lanthanum ions, but also acts as a photosensitive agent as the peptide nanostructure itself, thereby maximizing the luminous efficiency of lanthanum ions.

Example  2: various by mixing different illuminants Luminous color  Carrying Diphenylalanine Peptide  Nanostructure Manufacturing

In Example 1-1, red (peptide (FF) + 1,10-phenanthroline (PHEN) + europium (Eu), green (peptide (FF) + 1,10-phenanthroline (PHEN) + terbium ( Tb)), blue (peptide (FF) + salicylic acid (SA)) based on the results confirming that the light-emitting peptide nanostructures can be prepared, the organic photosensitive molecules (1,10-phenantrol binding to the peptide nanostructures By controlling the composition of lean, salicylic acid) and lanthanum ions (Europium, terbium), an attempt was made to prepare peptide nanostructures having various emission colors by adjusting the ratio of three primary colors. The structures were prepared and analyzed for their respective luminescence properties using a luminometer (Professional Scientific Instrument, DARSA PRO 5100 PL).

As a result, as shown in Figure 6b and 7, it can be confirmed that through the composition control of the precursor, it is possible to synthesize a peptide nanostructure having a variety of emission colors.

Example  3: luminous Peptide  Nano Structure Hydrogel  Manufacture and application

3-1. radiation Peptide  Nano Structure Hydrogel  Produce

Fmoc-diphenylalanine was used as a peptide precursor for synthesizing the light-emitting peptide hydrogel, and other precursors were used in the same material as in Example 1-1. Fmoc-diphenylalanine solution was prepared by dissolving 50 ml of Fmoc-diphenylalanine in 1 ml in 1,1,1,3,3,3-hexafluoro-2-propanol or dimethyl sulfoxide. A solution was prepared in which organic photosensitive molecules such as 4-acetylbiphenyl, 1,10-phenanthroline and salicylic acid and lanthanide salts such as europium chloride and terbium chloride were dissolved in methanol at 10 mM concentration, respectively.

The luminescent peptide hydrogel could be easily synthesized by diluting the prepared peptide solution, organophotoreceptor solution and lanthanum ion solution in distilled water by 1/2 to 1/25 times. Thereafter, the change in luminescence properties was observed using a fluorescence microscope (Nikon, Eclipse 80i).

As a result, as shown in Figure 8, unlike in the above Example 1-1 using diphenylalanine, the prepared Fmoc-diphenylalanine hydrogel to effectively combine with all the organic photosensitive molecules to improve the luminous properties of lanthanum ion I could confirm it.

3-2. radiation Peptide Hydrogel  Used pH Sensing

Since the luminescent properties of lanthanum-based ions react sensitively to environmental conditions such as polarity, hydrophobicity and pH around lanthanum ions, they can be usefully used as environmental sensors. Peptide hydrogel (peptide (FF) + 1, 10-phenanthroline (PHEN) + europium (Eu)) obtained through the above 3-1 was confirmed the applicability of the solution as a pH sensor.

To this end, 0.1 mol hydrochloric acid (pH <1), 0.1 mol acetic acid (pH 3.0 & 5.0), 0.1 mol Tris (pH 7.0 & 9.0), 0.1 M NaOH (pH> 12) in 1 ml of the peptide hydrogel prepared in 3-1 above. ) 100 ul each of the solution was added, and the change in luminescence properties was observed using a fluorescence microscope (Nikon, Eclipse 80i).

As a result, as shown in Figure 9, it was observed that the emission color of the light-emitting peptide hydrogel according to the pH, it was confirmed that the light-emitting peptide nanostructure according to the present invention can be usefully used as a pH sensor.

As described above in detail a specific part of the content of the present invention, for those skilled in the art, such a specific description is only a preferred embodiment, which is not limited by the scope of the present invention Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Figure 1 (a) is a chemical formula of the precursors used in the preparation of the light emitting peptide nanostructures according to the present invention, (b) is an organic light emitting molecule and lanthanum of the light emitting diphenylalanine peptide nanostructures prepared using the precursor The luminescence according to the ion composition is measured, and (c) is a fluorescence micrograph of the red, blue, and green peptide nanostructures of (b).

Figure 2 is an energy dispersive X-ray spectroscopy showing that the lanthanum ion is bonded to the light emitting diphenylalanine peptide nanostructure according to the present invention.

3 is a result of analyzing the crystal structure and shape of the luminescent diphenylalanine peptide nanostructure according to the present invention by X-ray diffraction and transmission electron microscope.

4 is a light emission spectrum measurement result showing the light emission characteristics of the light emitting diphenylalanine peptide nanostructure according to the present invention.

5 is a light emission spectrum analysis result for analyzing the mechanism of light emission characteristics of the light emitting diphenylalanine peptide nanostructure according to the present invention.

6 is a result of analyzing the emission color according to the composition of the luminescent diphenylalanine peptide nanostructure according to the present invention, (a) is the concentration of organophotosensitive molecules and lanthanum ion combined with the peptide nanostructure 32mM, (b) is the peptide nano It is a diagram showing the emission color according to the composition of the structure and the wavelength of the excitation light on the CIE colorimeter.

7 is a result of analyzing the emission color according to the composition of the light emitting diphenylalanine peptide nanostructure according to the present invention using a fluorescence microscope.

8 is a result of analyzing the luminescence properties according to the composition of the precursors (organic photosensitive molecules and lanthanum ions) used in the preparation of the luminescent Fmoc-diphenylalanine peptide hydrogel according to the present invention, the change in the luminescence properties by a fluorescence microscope The photograph was observed.

9 is a result showing that the light-emitting Fmoc-diphenylalanine peptide hydrogel according to the present invention can be used as a pH sensor, it is a photograph observing the change in the luminescence properties by a fluorescence microscope.

Claims (21)

Method for preparing a light-emitting peptide nanostructure, comprising the following steps: (a) dissolving the hydrophobic dipeptide or derivative thereof in an organic solvent; (b) preparing a solution in which at least one of lanthanum ions or salicylic acid is dissolved; And (c) the hydrophobic dipeptide or derivatives thereof prepared in step (a) is mixed with the solution prepared in step (b), so that the conjugate of the hydrophobic dipeptide or derivative thereof and the lanthanide ion or salicylic acid Preparing an assembled light-emitting peptide nanostructure. Method for preparing a light-emitting peptide nanostructure, comprising the following steps: (a) dissolving the hydrophobic dipeptide or derivative thereof in an organic solvent; (b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which a photosensitive agent is dissolved; And (c) mixing the hydrophobic dipeptides or derivatives thereof prepared in step (a) with the lanthanum ionic solution and sensitizer solution prepared in step (b) to prepare the hydrophobic dipeptides or derivatives, lanthanum ions and photosensitizer. Preparing a light-emitting peptide nanostructure in which the conjugate is self-assembled. A method of preparing a light emitting peptide nanostructure having a desired emission color in the visible region, comprising the following steps: (a) dissolving the hydrophobic dipeptide or derivative thereof in an organic solvent; (b) preparing a solution in which at least one of lanthanum ions is dissolved and a solution in which salicylic acid is dissolved; And (c) the hydrophobic dipeptide or derivative solution prepared in step (a) is mixed with the solution of step (b) to self-assemble, wherein the amount of each solution of step (b) to be mixed is adjusted to visible light Preparing a light emitting peptide nanostructure having a desired light emission color in the region. delete The hydrophobic dipeptiide or derivative thereof is NH ₂ -X ₁ -Y ₁ -COOH, NH ₂ -Phenylalanine-Phenylalanine-NH ₂ , fluorenylmethoxycarbonyl (FMOC) -X _2. -Y ₂ -COOH, (pentafluoro-phenyalanine)-(pentafluoro-phenylalanine) dipeptide, (iodo-phenylalanine)-(iodo-phenylalanine) dipeptide, (4-phenyl phenylalanine)-(4-phenyl phenylalanine) dipeptide, (p- nitro-phenylalanine)-(p-nitro-phenylalanine) dipeptide, phenylalanine-phenylalanine dipeptide, FMOC-phenylalanine-phenylalanine dipeptide, phenylalanine-phenylalanine-cystein tripeptide At this time, X ₁ or Y ₁ is selected from the group consisting of Ala, Val, Ile, Leu, Phe and Trp, X ₂ or Y ₂ is characterized in that selected from the group consisting of Gly, Ala, Leu, Phe and Lys box. The method of any one of claims 1 to 3, wherein the organic solvent is a volatile organic solvent. The method of claim 6, wherein the volatile organic solvent is 1,1,1,3,3,3-hexafluoro-2-propanol (1,1,1,3,3,3-hexafluoro-2-propanol, HFIP ), Trifluoroacetic acid and mixtures thereof. The method of any one of claims 1 to 3, wherein the lanthanum-based ions are terbium or europium. The method of claim 1, wherein the solvent for dissolving lanthanum-based ions or salicylic acid in step (b) is alcohol or water. The method of claim 2, wherein the photosensitizer is composed of 4-acetylbiphenyl, benzophenone, 1,10-phenanthroline, derivatives thereof, and mixtures thereof. Characterized in that it is selected from the group. The method of claim 2, wherein the solvent for dissolving the lanthanum-based ions and the photosensitizer in the step (b) is alcohol or water. The method of claim 3, wherein the solvent of step (b) is alcohol or water. 4. A method according to claim 1 or 3, further comprising mixing a photosensitive agent together in order to increase the efficiency of light emission in step (c). The method of claim 13, wherein the photosensitizer is selected from the group consisting of 4-acetylbiphenyl, benzophenone, 1,10-phenanthroline, derivatives thereof, and mixtures thereof. A light emitting peptide nanostructure prepared by the method of claim 1, wherein a combination of a hydrophobic dipeptide or a derivative thereof and the lanthanide ion or salicylic acid is self-assembled. A light emitting peptide nanostructure prepared by the method of claim 2, wherein a combination of a hydrophobic dipeptide or a derivative thereof, a lanthanide ion and a photosensitive agent is self-assembled. A light emitting peptide nanostructure having a desired emission color in the visible light region prepared by the method of claim 3. A sensor for measuring pH, comprising a light emitting peptide nanostructure of any one of claims 15 to 17. 19. The sensor of claim 18, wherein the light emitting peptide nanostructures are in the form of a hydrogel. 18. The method of measuring pH, using the light emitting peptide nanostructures of any one of claims 15 to 17. The method of claim 20, wherein the light emitting peptide nanostructure is in the form of a hydrogel.

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