KR101081953B1 - Apparatus for manufacturing cell culture scaffold - Google Patents

Abstract

PURPOSE: An apparatus for manufacturing a cell culture support is provided to enable rotational movement structure using two shafts. CONSTITUTION: An apparatus for manufacturing a cell culture support comprises: a heating part(170) having a syringe for storing biodegradable substances and a heating device for heating the biodegradable substances; a Z-axis motor(131) for moving the heating part in a Z-axis direction; a rotary table(140) for collecting the melt biodegradable substances; a movement body(180) for containing the rotary table; a X-axis motor(111) for moving the movement box in a X-axis direction; a Y-axis motor for moving the movement box in a Y-axis direction; a rotation motor(141) for rotating the rotary table; an angle adjusting table(150) which is contained in the movement box and supports the rotary table; and an angle adjusting motor(151) which is mounted at the outside of the movement box.

Description

Apparatus for manufacturing cell culture scaffold

The present invention relates to a device for producing a cell culture support, which is capable of moving or rotating in five axes to produce a cell culture support in various three-dimensional shapes.

In detail, the present invention relates to an apparatus for preparing a cell culture support by heating a biodegradable material, which is a raw material of the cell culture support, to form a binder in a liquid state, and then discharging the binder through a discharge nozzle and stacking the binder in a filament form.

In more detail, the position of the storage container (syringe and heating) in which the biodegradable substance is accommodated is changed in the Z-axis direction, and the table in which the molten biodegradable substance is collected is changed in the X- and Y-axis directions. In addition to the three-dimensional cell culture scaffold can be prepared, in order to manufacture the cell culture scaffold in a variety of shapes, a rotary rotary motor for rotating the table about the Z axis, and the table about the X axis It is for a device that is provided with a table angle control motor.

Tissue engineering is to collect the necessary tissue from the patient's body, separate the cells from the tissue pieces, and then proliferate the separated cells by the necessary amount through culture, and plant them on a porous biodegradable polymer scaffold for a certain period of time. After in vitro culture, the hybrid cell culture scaffold is transplanted back into the human body.

After transplantation, cells receive oxygen and nutrients by the diffusion of body fluid until new blood vessels are formed, and when blood vessels enter the body and supply blood, cells proliferate and differentiate to form new tissues and organs, and the polymer support is broken down. It is a technique that disappears.

Therefore, it is important to prepare biodegradable polymer cell culture scaffolds similar to biological tissues for these tissue engineering studies.

The main requirement of the material of the support used for the regeneration of human tissue should be mechanical strength enough to serve as a substrate or support so that the tissue cells can adhere to the material surface to form a three-dimensional structure of tissue. It should also serve as an intermediate barrier between the transplanted and host cells, which requires non-toxic biocompatibility without blood clotting or inflammatory reactions after transplantation.

In addition, when transplanted cells function and function as new body tissues, they must have biodegradability that can be completely degraded and disappeared in vivo within the desired time.

The present invention provides a manufacturing apparatus capable of moving in five axes by providing a linear movement to three axes of XYZ and a rotational movement about the X and Z axes, thereby providing not only cylindrical cell culture support but also cones, An object of the present invention is to provide a cell culture support for producing a cell culture support of various three-dimensional shapes such as spheres.

Through this, it is an object to be able to manufacture a cell culture support of various shapes that can be easily used in blood vessels, neural conduits, organs and the like.

The apparatus for producing a cell culture support according to the present invention comprises a heating unit having a syringe for storing a biodegradable material and a means for heating the biodegradable material; A Z-axis motor for moving the heating unit in the Z-axis direction; A rotary rotary table for collecting the molten biodegradable material extruded from the heating unit; A moving box for receiving the rotary rotary table; An X-axis motor for moving the moving box in the X-axis direction; A Y-axis motor for moving the moving box in the Y-axis direction; A rotary rotary motor for rotating the rotary rotary table; An angle adjusting table accommodated in the moving box and supporting the rotary rotary table; And an angle adjusting motor mounted outside the moving box and rotating the angle adjusting table about an axis positioned in a plane perpendicular to the Z axis direction.

And, the rotary rotary motor is characterized in that mounted on the lower side of the angle adjustment table in the moving box.

The angle adjusting table is connected to one side of the moving box and the rotary rotating table, and the angle adjusting motor rotates the angle adjusting table and the rotary rotating table simultaneously as the angle adjusting motor is driven.

And, the rotation of the angle adjustment table according to the drive of the angle adjustment motor, characterized in that made around the X axis.
Further, a movement base member positioned below the movement box to guide movement of the movement box in the X-axis direction, the movement base member includes an X-axis ball screw connected to the X-axis motor, and a movement of the movement box. An X-axis guide rail for guiding the movement is provided.
In addition, the Y-base guide rail for guiding the Y-axis direction movement of the movable base member and the movable box, and the Y-axis ball screw connected to the Y-axis motor are provided below the movable base member. .

In addition, the apparatus for producing a cell culture support according to another embodiment of the present invention comprises a heating unit having a syringe for storing a biodegradable material, and a means for heating the biodegradable material; A Z-axis motor for moving the heating unit in the Z-axis direction; A heating device that collects molten biodegradable material extruded from the nozzle of the heating unit and generates heat with the collected biodegradable material; An angle adjustment motor for rotating the heating device about an X axis; An X-axis motor for moving the heating device in the X-axis direction; And a Y-axis motor for moving the heating device in the Y-axis direction, wherein the heating device includes an upper plate in which the molten biodegradable material is collected, and a Peltier element provided below the upper plate to generate heat. And, it characterized in that it comprises a cooling fan for cooling the Peltier element.

By the production apparatus of the cell culture scaffold of the present invention as proposed, it is possible to implement a structure that moves along three axes and a structure that rotates around two axes, thereby moving / rotating in five axes There is an advantage that can be produced through the various three-dimensional cell culture support.

1 and 2 are a perspective view of the apparatus for producing a cell culture support according to the invention.
3 to 5 are front, right and left side views of the apparatus for producing a cell culture support according to the present invention.
6 to 9 are views for explaining the main configuration of the heating unit constituting the apparatus for producing a cell culture support according to the present invention.
10 is a perspective view of a heating apparatus according to the present invention.
11 is an exploded perspective view of the heating device.

Hereinafter, with reference to the accompanying drawings for the present embodiment will be described in detail. However, the scope of the inventive idea of the present embodiment may be determined from the matters disclosed by the present embodiment, and the inventive idea of the present embodiment may be implemented by adding, deleting, or modifying components to the proposed embodiment. It will be said to include variations.

1 and 2 are perspective views of the apparatus for producing a cell culture support according to the present invention, and FIGS. 3 to 5 are front views, right side views, and left side views of the apparatus for producing a cell culture support according to the present invention.

Each configuration will be described in detail with reference to the perspective view of the apparatus of the present invention shown in FIGS. 1 and 2, and in the parts requiring additional explanation, the drawings shown in FIGS. .

The apparatus for producing a cell culture support according to the present invention includes heating and spraying means for heating a raw material of biodegradable material and then discharging it through a spray nozzle, and a moving means for manufacturing the cell culture support in various three-dimensional shapes.

In detail, the cell culture support is prepared by melting the biodegradable polymer and extruding the molten biodegradable polymer in the form of a filament, and the biodegradable polymer raw material is accommodated in the heating unit 170.

Here, the heating unit 170 is provided with a cylindrical solution storage unit (cylinder 172, see Fig. 9) for accommodating the biodegradable polymer, in the space between the syringe 172 and the heating unit Hot wires may be formed. The heating unit 170 may include a temperature sensor capable of measuring an internal temperature and a pressure sensor capable of measuring an internal pressure.

In addition, the lower part of the heating unit 170 is formed with a precision nozzle for extruding the molten biodegradable polymer.

In addition, by collecting and stacking the molten biodegradable polymer extruded through the nozzle of the heating unit 170, a plurality of moving means for producing a cell culture support of various shapes is provided.

That is, the means for rotating the rotary rotary table 140 on which the prepared cell culture support is placed, the means for rotating the angle adjustment table 150 mounted below the rotary rotary table 140, and the rotary rotation Means for moving the moving box 180 on which the table 140 and the angle adjustment table 150 are seated on the X axis, and means for moving the moving box 180 to the Y axis.

In detail, the apparatus for manufacturing a cell culture support according to the present invention includes the moving box 180, the X-axis motor 111 for moving the moving box 180 to the X-axis, and the moving box 180 Y-axis motor 121 (refer to FIG. 4) for moving to a Y-axis, the angle adjustment table 150 mounted in the said moving box 180, and the rotary rotation table provided on the angle adjustment table 150. 140, a Z-axis motor 131 for moving the heating unit 170 to the Z-axis, and frames 101 and 102 forming a frame to support the above components.

The frame is bent in an L shape, and consists of a first frame 101 in a vertical direction and a second frame 102 in a horizontal direction. The Y-axis motor 121 and the Y-axis motor 121 are rotated by the Y-axis motor 121 as driving means for moving the moving box 180 in the Y-axis direction on the second frame 102. A ball screw 123 and a Y axis guide rail 122 for guiding the movement of the movable base member 110 to be moved according to the rotation of the Y axis ball screw 123 is provided.

The lower surface of the movable base member 110 is provided with a member formed with a screw thread that is engaged with the thread of the Y-axis ball screw 123, the Y-axis ball screw 123 is rotated along the ball screw 123 Will move. Therefore, the movable base member 110 moves in the + and + directions of the Y axis according to the rotational direction of the Y axis ball screw 123.

Here, the moving base member 110 is a member for supporting the moving box 180 from the lower side, when the moving base member 110 is moved to the Y axis in accordance with the drive of the Y-axis motor 121, The moving box 180 located on the moving base member 110 also moves on the Y axis.

And, on the moving base member 110, the X-axis motor 111 for moving the moving box 180 in the X-axis direction, and the X-axis ball screw to rotate in accordance with the drive of the X-axis motor 111 113 and an X-axis guide rail 112 for guiding the X-axis movement of the moving box 180 is provided. That is, the moving box 180 is seated on the X-axis guide rail 112, the lower surface of the moving box 180 is provided with a threaded member that is engaged with the thread of the X-axis ball screw 113, When the X-axis ball screw 113 is rotated by the driving of the X-axis motor 111 is moved in the X-axis direction along the X-axis guide rail 112.

As a result, the movable base member 110 moves along the Y-axis guide rail 122 by the driving of the Y-axis motor 121, and the movable box 180 located on the movable base member 110 is also present. It moves in the Y-axis direction. In addition, the moving box 180 is moved along the X-axis guide rail 112 in the X-axis direction by the driving of the X-axis motor 111. In addition, the movement in the Y-axis and X-axis directions of the movement box 180 enables movement in the Y-axis and X-axis directions of the rotary rotary table 140 mounted inside the movement box 180. As a result, the cell culture support may be manufactured in various shapes using the biodegradable polymer extruded through the nozzle of the heating unit 170.

In particular, in the present invention, the rotary culture motor for rotating the rotary rotary table 140 about the Z axis so that the cell culture support can be manufactured in various forms such as cylindrical, conical, oval, and Table angle adjustment motor 151 for rotating the angle adjustment table 150 mounted below the rotary rotary table 140 to the center of the X-axis is provided.

The moving box 180 has a rectangular shape and only a part of side surfaces thereof is formed in an open shape as a whole, and inside the moving box 180, a rotary rotary motor 141 for rotating the rotary rotary table 140 is provided. The angle adjusting table 150 positioned above the rotary rotating motor 141 and the rotary rotating table 140 positioned on the angle adjusting table 150 are mounted. In addition, the rotary shaft through which the rotational force of the rotary rotary motor 141 is transmitted is connected to the rotary rotary table 140 through the angle adjustment table 150.

And, one side of the moving box 180 is equipped with a table angle adjustment motor 151 for rotating the angle adjustment table 150, the rotating shaft is transmitted to the rotational force of the table angle adjustment motor 151 is the movement It penetrates the side of the box 180 and is connected to the side of the angle adjustment table 150. Therefore, when the table angle adjustment motor 151 is driven, the rotational force is transmitted to the angle adjustment table 150 along the rotation axis such that the angle adjustment table 150 rotates clockwise or counterclockwise about the X axis. do.

In addition, the first frame 101 formed in the vertical direction is mounted with a Z-axis motor 131 for moving the heating unit 170 in the Z-axis direction. The Z-axis guide for guiding the Z-axis ball screw 133 to which the rotational force of the Z-axis motor 131 is transmitted and the moving block 160 moving in the Z-axis direction along the Z-axis ball screw 133. The rail 132 is mounted in the Z-axis direction.

The moving block 160 is an auxiliary means for allowing the heating unit 170 to move in the Z-axis direction according to the driving of the Z-axis motor 131. The heating unit 170 has an elevating plate 162. ) Is mounted and a connection plate 161 coupled to the elevating plate 162 is provided in the moving block 160. Through this, the moving block 160 moves in the Z-axis direction according to the driving of the Z-axis motor 131, and the lifting plate 162 and the heating unit 170 are moved by the movement of the moving block 160. ) Ascends. In addition, it is possible to finely control the Z-axis movement of the heating unit 170 by adjusting the lifting of the connecting plate 161 and the lifting plate 162. A micrometer gauge 163 may be mounted on the upper portion of the connection plate 161 to control the lifting of the lifting plate 162.

In addition, an upper portion of the heating unit 170 is provided with an air control valve 171 for adjusting the pressure for extruding the molten biodegradable polymer. A pneumatic injection hose (not shown) is connected to the air control valve 171 to constantly adjust the pressure for extruding the molten biodegradable polymer or to vacuum the syringe 172 inside the heating unit 170 in a vacuum state. It can also be made to prevent the molten biodegradable polymer from being discharged through the nozzle. In the case of varying the size of the precision nozzle for extruding the molten biodegradable polymer, it is possible to increase the precision of the filament constituting the cell culture support, and also through three-axis linear movement and two-axis rotational movement The support can be prepared while moving along the axis.

 In particular, by controlling the internal pressure of the syringe 172 by the air control valve 171, it is possible to control the derivation rate of the filament constituting the cell culture support.

The operation of the apparatus for producing a cell culture support of the present invention having the above-described configuration will be described, and the following operation description is merely an example, and therefore the scope of the present invention should not be limited.

For example, a case of preparing a cylindrical cell culture support will be described. In the process of manufacturing a spherical cell culture support, as described above, the extraction speed of the biodegradable polymer extruded through the heating unit 170 can be controlled by the extrusion pressure by the air control valve 171, Z-axis movement of the heating unit 170, the rotary rotary table 140 and the angle adjustment table 150, X-axis movement, Y-axis movement, the rotation about the X-axis center is performed. The rotary rotary table 140 may also be rotated about the Z axis.

Cylindrical cell culture support is dropped on the rotary rotary table 140, the molten binder is rotated, the rotary rotary table 140 is rotated, the movement in the X-axis and Y-axis continuously. In addition, when the temperature of the melted binder decreases, the filament constituting the cell culture support is formed, and the heating unit 170 gradually moves in the Z-axis direction in order to sequentially stack the filaments.

During the manufacturing process of the cell culture support, it is possible to determine the falling position of the molten binder more precisely while the fine adjustment of the angle adjustment table 150 around the X-axis.

That is, the position where the molten biodegradable polymer falls on the rotary rotary table 140 includes the rotation of the rotary rotary table 140, the X and Y axis movements of the rotary rotary table 140, and the angle adjustment table ( 150 and the rotary rotary table 140 may be determined by the rotation about the X-axis center.

In addition, the height of the molten biodegradable polymer falling on the rotary rotary table 140 may be achieved by the Z-axis movement of the heating unit 170.

According to the apparatus for producing a cell culture support according to the present invention, it is possible to linearly move in three axes and to rotate around two axes, and to support not only a cylindrical shape but also a three-dimensional cell culture support such as a spherical shape and a conical shape. There is an advantage to manufacture. That is, by the linear and rotational movement in five axes, it is possible to produce a cell culture support of various three-dimensional shape.

6 to 9 are views for explaining the main configuration of the heating unit constituting the apparatus for producing a cell culture support according to the present invention.

The heating unit 170 is formed in a cylindrical shape as a whole, and the syringe 172 for accommodating the biodegradable polymer 20 is mounted in the heating unit 170. The upper portion of the syringe 172 has a syringe head portion 173 having a larger size than the syringe body portion, and the syringe head portion 173 has a cap 30 for sealing the inside of the syringe. Is combined.

Cap caps 31 are formed at both ends of the cap 30 so as to be slidably coupled to the syringe head 173, and the caps 31 may be manufactured by cutting. Can be. In addition, an air control valve 171 is formed on the cap 30 to adjust the pressure inside the syringe 172, and the air control valve 171 is connected to an external device through an air injection hose. .

In addition, the lifting plate 162 is coupled to the rear of the heating unit 170 to assist the Z-axis movement of the heating unit, and the lower side of the heating unit 170 is biodegradable in the interior of the syringe 172. It is equipped with a precision nozzle 174 from which the polymer is extruded.

In addition, a heating wire is formed between the heating unit 170 and the syringe 172, and the biodegradable polymer in the syringe 172 is melted by the heating wire.

10 is a perspective view of a heating apparatus according to the present invention, Figure 11 is an exploded perspective view of the heating apparatus.

The heating apparatus shown in FIGS. 10 and 11 may be mounted in place of the rotary rotary table 140 described above with reference to FIGS. 1 to 3 or may be manufactured as a component mounted on the rotary rotary table 140. It is a means for applying a predetermined heat to the culture support.

That is, the high temperature molten biodegradable polymer extruded through the precision nozzle 174 may fall on the rotary rotary table 140, and then may be shrunk by a temperature difference. This is because the surface temperature is low when the rotary rotary table 140 is made of a metallic material.

In order to prevent such a phenomenon in advance, it is disclosed with respect to the heating device 200 that can be mounted in place of the rotary rotary table 140, or may be mounted on the upper surface of the rotary rotary table 140.

The heating device 200 includes a Peltier element 201 as a thermoelectric member, and the heat dissipation part of the Peltier element 201 faces the upper side, and the cooling unit of the Peltier element 201 faces the lower side. In addition, a cooling fan 221 for assisting cooling is mounted below the Peltier element 201, and the cooling fan 221 is provided in the cooling box 220.

A bottom plate 240 is mounted below the cooling fan 221, and side plates 230 are mounted on both sides of the cooling fan 221.

In addition, an upper plate 210 is provided on the Peltier device 201 to support the cell culture support.

By the heating apparatus 200, even if the biodegradable polymer melted at a high temperature is extruded, since the heat falls on the plate to generate heat, it is possible to improve the gradation phenomenon of the filament due to a sudden temperature difference.

According to the apparatus for producing a cell culture support of the present invention as described above, it is possible to manufacture a scaffold of various three-dimensional shape, by using it to replace the damaged cartilage of the human body, or soft tissue to supply nutrition to the cartilage In addition, the skin can be cultured and transplanted to recover even when the cartilage itself and some or all of its surrounding tissues are lost.

101: first frame 102: second frame
110: moving base member 111: X axis motor
112: X axis guide rail 113: X axis ball screw
121: Y axis motor 122: Y axis guide rail
123: Y-axis ball screw 131: Z-axis motor
132: Z axis guide rail 133: Z axis ball screw
140: rotary rotary table 141: rotary rotary motor
150: angle adjustment table 151: table angle adjustment motor
160: moving block 170: heating unit
180: moving box 200: heating device

Claims (7)

A heating unit having a syringe in which a biodegradable substance is stored and a means for heating the biodegradable substance;
A Z-axis motor for moving the heating unit in the Z-axis direction;
A rotary rotary table for collecting the molten biodegradable material extruded from the heating unit;
A moving box for receiving the rotary rotary table;
An X-axis motor for moving the moving box in the X-axis direction;
A Y-axis motor for moving the moving box in the Y-axis direction;
A rotary rotary motor for rotating the rotary rotary table;
An angle adjusting table accommodated in the moving box and supporting the rotary rotary table; And
And an angle adjusting motor mounted to the outside of the moving box and rotating the angle adjusting table about an axis positioned in a plane perpendicular to the Z-axis direction. The method of claim 1,
The rotary rotary motor is a cell culture support device, characterized in that mounted on the lower side of the angle adjustment table in the moving box. The method of claim 1,
The angle adjustment table is connected to one side of the moving box and the rotary rotary table,
The angle control motor is driven, the apparatus for producing a cell culture support, characterized in that for rotating the angle adjustment table and the rotary rotary table at the same time. The method of claim 3, wherein
The rotation of the angle adjustment table according to the drive of the angle adjustment motor, the production apparatus of the cell culture support, characterized in that made around the X axis. The method of claim 1,
A movement base member positioned below the movement box to guide movement of the movement box in the X-axis direction,
The moving base member is provided with an X-axis ball screw connected to the X-axis motor, and an X-axis guide rail for guiding the movement of the moving box is provided with a cell culture support. The method of claim 5, wherein
Under the moving base member, a cell culture comprising a Y-axis guide rail for guiding the Y-axis movement of the moving base member and the moving box, and a Y-axis ball screw connected to the Y-axis motor Apparatus for producing a support. A heating unit having a syringe in which a biodegradable substance is stored and a means for heating the biodegradable substance;
A Z-axis motor for moving the heating unit in the Z-axis direction;
A heating device that collects molten biodegradable material extruded from the nozzle of the heating unit and generates heat with the collected biodegradable material;
An angle adjustment motor for rotating the heating device about an X axis;
An X-axis motor for moving the heating device in the X-axis direction; And
Y-axis motor for moving the heating device in the Y-axis direction; includes,
The heating apparatus includes a top plate in which the molten biodegradable material is collected, a Peltier element provided under the top plate to generate heat, and a cooling fan for cooling the Peltier element. Apparatus for producing a support.

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