KR101045397B1 - Process for preparing refined squid oil having the high content of omega-3-fatty acid - Google Patents

Abstract

The present invention relates to a method for producing refined squid oil having a high content of omega-3 fatty acids. Purified squid oil prepared according to the present invention has a low acid value by performing a low temperature solvent fractionation and molecular distillation process of the squid oil purified in a general process, the acid value is low, about 40 to 50% omega-3 fatty acid (DHA about 24 to 30 %, EPA about 11-16%), heavy metals such as lead (Pb), cadmium (Cd), arsenic (As) and the like are not detected, reducing serum total cholesterol and serum triglycerides.

Description

Process for preparing refined squid oil having the high content of omega-3-fatty acid}

The present invention relates to a method for producing refined squid oil having a high content of omega-3 fatty acids.

The EPA / DHA-containing refined fish oil market in Korea was about 25 billion won including imports in 2000, and is increasing with the growth of the dietary supplement market. However, imports account for about 15 billion won, occupying 60% of the domestic market, and the rate is expected to increase further.

Imported refined fish oil is mainly imported from Japan, Norway, Germany, Australia, Chile, etc. Among them, especially Japanese imported products have a relatively high EPA / DHA content of around 45% and high quality in terms of odor and oxidation stability. It is known to have high demand in Korea. In addition, other imports are relatively inexpensive, increasing the domestic market share. Accordingly, the location of domestic refined fish oil and manufacturing industry is gradually narrowing, and it is urgent to develop independent technology for producing refined fish oil of high quality comparable to imported products, especially Japanese products.

Common refining methods for fish oils include degumming, deoxidation, decoloring, chilling and deodorization. In degumming and deoxidation, phospholipids such as phospholipids are precipitated and removed using hot water and phosphoric acid.In the process of decolorization, acidic clay, activated clay and activated carbon are used to remove pigments. Soak and remove, and during the deodorization process, deodorizing and deodorizing components using water vapor in a high vacuum deodorization pipe for a certain temperature and a certain time. However, the general purification process has a limit in improving the content of omega-3 fatty acids by remaining at the level of removing saturated fatty acids and a portion of monounsaturated fatty acids rather than increasing the content of omega-3 fatty acids such as DHA / EPA.

The raw materials of currently marketed refined fish oil mainly include tuna oil, sardine oil, salmon oil, etc. The refined fish oil contains about 30% of omega-3 fatty acids such as DHA and EPA. Therefore, the refined fish oil is used as a raw material of health functional food. However, when ingesting purified fish oil containing omega-3 fatty acids as a health functional food, there is a problem that a relatively large amount of intake is expected to expect a physiological functional effect within a short time. Therefore, if the same physiological function can be obtained even with a relatively small amount of ingestion, it can be more easily used, and it is thought that it will be helpful for the activation of the dietary supplement market and the health of modern people.

On the other hand, the catch of squid has averaged 187,000 tons in Korea's coastal mountains for the past three years (aquatic fish catch statistics, 2008). It is being disposed of and causes environmental pollution. Some of the squid guts are used directly for fish farming or as lipid sources of feed by extracting lipids. As a by-product of squid processing, squid intestine contains 20% to 30% of squid oil, and squid oil contains 21% or more of DHA and 10% or more of omega-3 fatty acid only in crude oil before refinement. Therefore, squid oil is highly valuable as a raw material for the manufacture of refined fish oil, as well as a high value-added dietary supplement, and can be easily collected from squid processed by-products such as squid intestines. It is very suitable as a raw material.

In recent years, as the importance of marine biological resources has been raised and awareness has been raised, technology development for high value-adding of these resources has also been actively carried out. Purification technology of refined fish oil has been developed to develop physiologically functional polyunsaturated fatty acids (DHA / EPA). Etc.) can easily take a product containing a large amount. However, there is no comparative advantage in competition with imports for quality and price.

Therefore, there is a demand for the development of refined fish oil which can increase the content of omega-3 fatty acid in refined fish oil and obtain the same physiological function even with a small amount of ingestion.

While the present inventors have been studying a method for increasing the content of omega-3 fatty acids in refined fish oil, the purified squid oil has been added to low acid value and low omega-3 fatty acid which have been subjected to low temperature solvent fractionation and molecular distillation process. It was found to contain very high and reduced serum total cholesterol and serum triglycerides, completing the present invention.

The present invention is to provide a method for producing purified squid oil having a high content of omega-3 fatty acids.

The present invention

1) extracting, degumming, deoxidizing and decolorizing squid oil from squid gut;

2) separating the low-temperature solvent by adding the bleaching oil prepared in step 1) to the organic solvent;

3) vacuum deodorizing the fractionating oil;

4) molecular distillation of the deodorized oil; And

5) It provides a method for producing purified squid oil having a high content of omega-3 fatty acid, comprising the step of filtering the adsorbent to the molecular distilled squid oil.

Hereinafter, the present invention will be described in detail step by step.

Step 1) in the method for preparing purified squid oil of the present invention is a general refining process of squid oil, and first concentrated domestically prepared squid gut under agitation at 100 to 150 ° C. for 4 to 8 hours under vacuum, followed by stationary cooling for 2 hours or more. The squid oil is extracted by suction separating the upper oil layer with a vacuum pump. The squid oil is degumming using MSG (monosodium glutamate, L-glutamate) by-product, phosphoric acid or citric acid. The MSG by-product, phosphoric acid or citric acid comprises about 10 to 20% by weight relative to 100% by weight of squid crude oil. The acid value of the degumming oil was measured, and a 15% caustic soda (NaOH) aqueous solution was added to the acid value, followed by a deoxidation treatment while stirring at 15 rpm for 10 to 20 minutes. Then, when the acid value is 0.5 or less, the deoxidized oil is separated from the insoluble portion by centrifugation, and the separated deoxidized oil is heated to 75 to 85 ° C, and 5 to 15% by weight of flushed water is added for 25 to 35 minutes. After washing with water, centrifugation to prepare deoxidized oil from which NaOH component was removed. After the deoxidized oil was completely dehydrated at 110 to 120 ° C. under vacuum, the vacuum was released, an adsorbent was added, and then reacted with stirring at 110 to 120 ° C. for 30 minutes under vacuum, followed by cooling to 70 to 75 ° C. to release the vacuum. It is then filtered to decolorize. The adsorbent is preferably added to about 3 to 5% by weight of deoxidized oil, the type of adsorbent is at least one selected from the group consisting of MiZuka life F-1G, magnesol (magnesium silicate), acid clay, activated clay and activated carbon This is preferred.

The step 2) is a step of fractionating the bleaching oil at low temperature, adding 2 to 4 times (volume) of organic solvent to the bleaching oil, preferably 3 times (volume), and -10 to -40 ° C under vacuum of 400 to 500 mmHg. After cooling to 8-8 hours, solvent fractionation was performed to immerse and separate saturated fatty acids and monounsaturated fatty acids having high melting points, and to prepare omega-3 fatty acids such as DHA and EPA, which are relatively low melting points. Concentrate separately on top layer. Then, the concentrate is heated to 30 to 35 ℃ to evaporate the solvent to prepare a fractionated oil. The organic solvent includes one selected from the group consisting of n-hexane, acetone, benzene, ethanol and isopropyl alcohol, and acetone is preferable in the present invention.

Step 3) is a step of vacuum deodorizing the fractionated oil, the fractionated oil is added to the deodorizing tank and heated to 170 to 190 ℃, preferably 175 to 185 ℃ under a vacuum degree of 2.0 to 4.0 torr, preferably 2.5 to 3.5 torr Deodorize for 5-6 hours.

Step 4) is a step of molecular distillation of the deodorized oil. The deodorized oil is fed into a molecular distiller and centrifugal molecular distillation for 1 to 2 hours under vacuum at 100 ° C.

Step 5) is a step of filtering by adding an adsorbent to the molecular distilled squid oil, the molecular distillation oil is transferred to a filter tank and cooled to 75 to 85 ℃, then added to the adsorbent and reacted at this temperature for 20 to 40 minutes and then nitrogen gas Is filtered while feeding at a pressure of 0.5 to 1.0 kg / cm 2. The adsorbent is added in an amount of about 2 to 5% by weight, preferably about 3 to 4% by weight of the deodorizing oil. Examples of the adsorbent include MiZuka life F-1G, magnesol (magnesium silicate), acidic clay, activated clay and activated carbon. At least one selected from the group consisting of is preferable.

Purified squid oil prepared according to the method has a low acid value, contains about 40 to 50% of omega-3 fatty acids (about 24 to 30% of DHA, about 11 to 16% of EPA), lead (Pb) and cadmium (Cd). ) And heavy metals such as arsenic (As) are also not detected, reducing serum total cholesterol and serum triglycerides.

As described above, the refined squid oil according to the present invention has excellent acid oxidation stability due to low acid value, and contains at least 10% to 20% higher Omega-3 fatty acids (DHA and EPA) than conventional refined fish oil. Even if you consume a relatively small amount of refined fish oil, you can expect more than the same level of physiological functionality. In addition, since the purified squid oil according to the present invention can be easily obtained from squid processed by-products such as squid internal organs, it is relatively economical and environmentally friendly since it is relatively easy to supply raw materials and is a high value-added material using by-products.

Purified squid oil according to the present invention can be used as a health food useful for improving hyperlipidemia. When the refined squid oil according to the present invention is used as a food additive, the refined squid oil may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the manufacture of food or beverage, the refined squid oil of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or for health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of the food to which the substance may be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, various soups, beverages, tea, and drinks. Alcoholic beverages and vitamin complexes, and includes all of the health foods in the conventional sense.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example  One  : From squid oil  refine Squid oil  Produce

Domestic cuttle fish (cuttle fish, Sepia) esculenta ) viscera 8 M / T were added to the reactor (10 M / T) and concentrated with stirring at 100-150 ° C. for 4-8 hours under vacuum. Thereafter, the mixture was left to cool for 2 hours or more, and the squid oil was extracted by suction separating the upper oil layer with a vacuum pump.

600 kg MSG (monosodium glutamate, L-glutamate) by-product and 1,200 kg of water were added to 4,000 kg of the squid oil, and the reaction was stirred at 55 to 65 ° C. for about 1 hour. Phospholipids in crude oil are then translocated from lipophilic to hydrophilic. After standing for 10 to 12 hours, sludge and stick water were sequentially removed to prepare degumming oil.

The temperature of the reactor was set at 70 to 80 ° C., and the acid value of the degumming oil was measured, and a 15% caustic soda (NaOH) aqueous solution was added to the acid value, followed by deoxidation while stirring at 15 rpm for 10 to 20 minutes. Then, when the acid value was 0.5 or less, the deoxidized oil was separated from the insoluble portion by centrifugation, and the separated deoxidized oil was received in a washing tank. The deoxidized oil was heated to 75 to 85 ° C., washed with water for 25 to 35 minutes by addition of 5 to 15% by weight of washed water, and then centrifuged to prepare deoxidized oil from which the NaOH component was removed.

After the deoxidized oil was completely dehydrated at 110 to 120 ° C. under vacuum, the vacuum was released, and acidic clay (Daeil Chemical Co., Ltd.) was charged with about 3 to 5 wt% of deoxidized oil, followed by stirring at 110 to 120 ° C. under vacuum for 30 minutes. Reacted. After cooling to 70 to 75 ℃ to release the vacuum and filtered to prepare a bleaching oil.

Three times of acetone was added to the bleaching oil, cooled to -10 to -40 ° C under a vacuum of 400 to 500 mmHg, and then fractionated for 8 to 16 hours to immerse and separate saturated fatty acids and monounsaturated fatty acids having a relatively high melting point. , Omega-3 fatty acid components such as polyunsaturated fatty acids, DHA and EPA, which have relatively low melting points, were concentrated on the upper layer. Then, the concentrate was heated to 30 to 35 ℃ to evaporate the solvent to prepare a fractionated oil.

350-400 kg of fractionated oil was added to a deodorizing bath, heated to 175-185 ° C. under a vacuum degree of 2.5-3.5 torr, deodorized for 5 to 6 hours, and then fed into a molecular distiller and centrifuged at 100 ° C. for 1 to 2 hours under vacuum. Molecular distillation.

After the molecular distillation oil was transferred to a filtration tank and cooled to 75 to 85 ° C., an adsorbent (MiZuka life F-1G, Japan) was added at 3 to 5% by weight of the deodorized oil. The reaction was carried out at this temperature for 20 to 40 minutes, followed by filtration while feeding nitrogen gas at a pressure of 0.5 to 1.0 kg / cm 2 to obtain about 40 to 50% omega-3 fatty acids (about 24 to 30% DHA, about 11 to EPA). 16%) to prepare refined squid oil.

Chemical characteristics of the refined squid oil prepared by the purification process are shown in Table 1, and the fatty acid composition is shown in Table 2.

Chemical Characteristics of Squid Oil by Purification Process Acid
(AV, mg / g) Omega-3 Fatty Acids Heavy metals (mg / kg) moisture
(%) DHA EPA Lead (Pb) Cadmium (Cd) Arsenic (As) Squid Crude Oil 18.67 21.70 10.0 0.042 0.031 0.057 2.32 Degumming oil 19.39 21.75 10.41 - - 0.072 0.78 Deoxidized oil 0.17 25.12 11.17 0.04 0.031 0.043 1.62 Bleach 0.27 24.46 10.76 0.039 0.03 0.041 1.05 Classification oil (1st) 3.18 24.66 11.89 - - - 0.66 Classification oil (2nd) 2.01 25.34 12.51 - - - 1.23 Classification oil (3rd) 1.3 25.06 12.56 - - - 1.28 Classification oil (4th) 0.95 25.91 13.21 - - - 0.98 Classification oil (5th) 0.72 27.02 14.37 - - - 1.09 Refined Squid Oil 0.5 30.20 15.02 - - - 0.9

As shown in Table 1, DHA and EPA of squid crude oil were 21.7% and 10.0%, respectively, and DHA and EPA of refined squid oil were 30.2% and 15.0%, respectively, about 10% DHA and EPA of refined squid oil. It rose about 5%. In addition, the acid value was 18.67% in squid crude oil and 0.5% in refined squid oil, significantly lower in squid crude oil. In addition, heavy metals contain lead (Pb), cadmium (Cd), and arsenic (As) of 0.042 mg / kg, 0.031 mg / kg, and 0.057 mg / kg, respectively. Kg, cadmium 2.0 mg / kg, mercury 0.5 mg / kg) and were not detected in fractionated and refined squid oil.

Fatty Acid Composition of Squid Oil by Refinery Process ECL value Abbreviation fatty acid Fatty Acid Composition by Purification Process (%) One 2 3 4 5 6 7 8 9 13.78 i-14: 0 12 or 11-methyltridecanoic acid 2.94 2.92 2.71 2.76 2.40 2.37 2.35 2.34 2.31 15.63 3-OH-14: 0 3-hydroxytetradecanoic acid 3.87 3.93 3.80 3.78 4.66 4.78 4.82 4.74 4.60 15.88 i-16: 0 14-methylpentadecanoic acid 16.02 15.35 15.05 15.35 10.16 9.92 10.01 10.22 9.86 17.42 ni - 0.96 0.99 0.97 0.91 1.44 1.46 1.35 1.32 1.45 17.66 18: 3 w6 Cis-6,9,12-octadecatenoic acid 16.71 16.84 16.91 16.89 16.29 16.41 16.81 17.17 16.32 17.86 18: 2 w6 Cis-6,12-octadecadienoic acid 3.33 3.20 3.07 3.16 1.59 1.45 1.50 1.57 1.52 19.26 20: 5 w3 Cis-5,8,11,14,17-eicosapentaenoic acid 11.60 11.77 11.66 11.55 16.21 16.44 15.92 15.59 15.95 19.61 20: 2 w6 Cis-11,14-eicosadienoic acid 6.76 6.81 6.99 6.94 5.10 4.99 5.20 5.39 5.32 21.05 22: 6 w3 Cis-4,7,10,13,16,19-docosahexaenoic acid 24.36 24.40 25.25 25.02 30.58 30.89 30.50 30.58 30.09 21.54 22PUFA - 3.24 3.27 3.46 3.45 1.88 1.74 1.84 1.87 1.92 Total fatty acids 89.79 89.49 89.86 89.82 90.32 90.45 90.30 90.80 89.33 n-3 fatty acids (DHA + EPA) 35.96 36.17 36.91 36.57 46.79 47.33 46.42 46.17 46.04 ※ 1. squid crude oil; 2. Degumming oil; 3. deoxidized oil; 4. bleaching oil; 5, fractionated oil (primary);
6. fractionated oil (secondary); 7. fractionated oil (tertiary); 8. fractionated oil (quad); 9. Refined Squid Oil.

As shown in Table 2, the content of omega-3 fatty acids (DHA + EPA) increased to about 36.0% in squid crude oil, about 46.0% in refined squid oil, and about 10% in refined squid oil. Saturated fatty acids, on the other hand, tended to decrease. In the case of 14-methylpentadecanoic acid, about 16.0% of crude squid oil and about 9.9% of refined squid oil decreased by about 6%.

Experimental Example  One  : Purification by low temperature solvent fractionation Squid oil  Acid, peroxide and omega-3 fatty acids ( DHA  And EPA Content measurement

The acid value, peroxide value and omega-3 fatty acid (DHA and EPA) contents of the purified squid oil according to the low temperature solvent fractionation treatment from the squid oil were measured. The results are shown in Table 3.

Acid, Peroxide, and Omega-3 Fatty Acids (DHA and EPA) Contents of Refined Squid Oils with Low Temperature Solvent Classification Acid value (AV) Peroxide Value (POV) Omega-3 Fatty Acid Content (%) DHA EPA Squid crude oil (crude) 28.72 3.1 21.1 12.6 Refined Squid Oil
(Differential Processing) 0.57 2.14 20.9 11.8 Refined Squid Oil
(Low Temperature Solvent Classification) 0.50 1.97 30.2 15.0

As shown in Table 3, the acid value (AV) and peroxide value (POV) of crude squid crude oil were 28.72 and 3.1, respectively, while the fractionated unrefined refined squid oil was lowered to 0.57 and 2.14, respectively. Treated refined squid oil had the lowest levels of 0.50 and 1.97, respectively. In addition, the content of DHA and EPA was 21.1% and 12.6% for squid crude oil, and 20.9% and 11.8% for unrefined refined squid oil, respectively. However, 30.2% and 15.0% of the crude squid oil treated with low temperature solvent fractions showed 30.2% and 15.0%, respectively. As described above, the low-temperature solvent fractionated refined squid oil of the present invention contains more than 45.2% of omega-3 fatty acids (DHA and EPA), compared to the fractionated refined squid oil (32.7%). EPA) content increased by more than 12.5%.

Experimental Example  2  : Purification according to molecular distillation Squid oil  Acid and omega-3 fatty acids ( DHA  And EPA Content measurement

When fractionated oil was subjected to molecular distillation, the acid value and the content of omega-3 fatty acids (DHA and EPA) of squid oil were measured before and after treatment. Omega-3 fatty acids (DHA and EPA) content of conventional refined fish oil, refined tuna oil, refined sardine oil, and refined salmon oil were measured and compared. The results are shown in Table 4.

Acid Value and Omega-3 Fatty Acid (DHA and EPA) Contents of Refined Squid Oil with and without Molecular Distillation Acid value (AV) Omega-3 Fatty Acid Content (%) DHA EPA Refined Squid Fractional Oil 0.80 27.0 ± 0.1 14.8 ± 0.1 Refined Squid Molecular Distillate Oil 0.23 27.4 ± 0.2 14.7 ± 0.2 Refined Tuna Milk - 25.0 ± 0.0 5.0 ± 0.0 Refined Sardine Oil - 12.0 ± 0.0 18.0 ± 0.0 Refined Salmon Oil - 12.0 ± 0.0 18.0 ± 0.0

As shown in Table 4, the acid value of the purified squid fractionated oil is 0.8, and the acid value of the purified squid oil after molecular distillation is significantly reduced to 0.23, indicating that molecular distillation has a great effect on maintaining the oxidative stability of the purified squid oil. In addition, the content of omega-3 fatty acids (DHA and EPA) also showed an equivalent or more content after molecular distillation.

In addition, the content of omega-3 fatty acids (DHA and EPA) of the refined squid oil molecularly distilled according to the present invention is about 40-50% (DHA about 24-30%, EPA about 11-16%), while refined tuna oil DHA 25% and EPA 5%, refined sardine oil or refined salmon oil is 12% DHA and 18% EPA, the content of omega-3 fatty acids (DHA and EPA) is about 30%, the refined squid oil of the present invention Omega-3 fatty acids (DHA and EPA) content is at least 10% and up to 20% higher than conventional refined fish oil. Therefore, the refined squid oil according to the present invention is expected to be able to expect the same level or more of physiological functionality even if the intake of a relatively small amount than conventional refined fish oil.

Experimental Example  3  : Tablet of the present invention Squid oil  Physiological Functional Check

In order to confirm the physiological function of the purified squid oil having a low acid value and a high content of omega-3 fatty acids (DHA and EPA) according to the present invention, the following experiment was performed.

The test subjects were physically examined to meet the test requirements, and the Chinese were serum total cholesterol ≥5.2 mmol / L and triglyceride ≥1.65 mmol / L, and 104 persons including 55 women and 49 men aged 34-65 years. It was targeted.

First, a capsule (500 mg, capsule base: gelatin, glycerin, purified water) containing the purified squid oil prepared in Example 1 was prepared by a conventional method. Subjects were given 6 capsules daily for 60 days. Serum total cholesterol and serum triglycerides were measured before and after taking the capsule. In contrast, the blank control was applied. Serum total cholesterol and serum triglyceride measurement results of the purified squid oil of the present invention are shown in Table 5 and Table 6, respectively.

Serum total cholesterol of purified squid oil of the present invention (unit: mmol / L) Number of people Before tasting After tasting P value (self) % Reduction
(itself) Contrast 52 6.37 ± 1.11 6.40 ± 1.05 0.459 -0.47 Test 52 6.38 ± 0.85 5.65 ± 0.70 0.000 ** 11.2 P value (morning) - 0.977 0.000 ## - - % Reduction
(Morning) - - 11.7 - -

※ **: Self comparison before and after experiment, p <0.01

   ##: comparison with contrast, p <0.01

Serum triglycerides of the purified squid oil of the present invention (unit: mmol / L) Number of people Before tasting After tasting P value (self) % Reduction
(itself) Contrast 52 2.66 ± 1.30 2.70 ± 1.20 0.211 -1.5 Test 52 2.64 ± 0.85 2.10 ± 0.98 0.000 ** 21.1 P value (morning) - 0.947 0.006 ## - - % Reduction
(Morning) - - 22.2 - -

※ **: Self comparison before and after experiment, p <0.01

   ##: comparison with contrast, p <0.01

As shown in Table 5 and Table 6, the comparison result of the test tank before and after the test, the serum total cholesterol level of the purified squid oil of the present invention showed a decrease percentage of 11.2% by 0.73 mmol / L, the serum triglyceride level 0.54 mmol / L was shown as a reduced percentage of 21.1%, and the high density lipoprotein cholesterol level was not significantly different (P> 0.05). In addition, as a result of comparing the test and control tanks, the serum total cholesterol level was decreased by 0.75 mmol / L after the experiment was 11.7%, and the serum triglyceride level was 22.2% by 0.60 mmol / L. All showed distinct differences (P <0.01). However, high density lipoprotein cholesterol levels did not show a significant difference (P <0.05).

During the experimental period, the subjects' mental, sleep, food, urine and blood pressure clinical indicators showed no abnormalities and no other adverse reactions. Therefore, it can be seen that the purified squid oil capsule of the present invention has a secondary function of reducing blood lipid and has no adverse reaction to the health of the organism.

Purified squid oil prepared according to the present invention has a low acid value by performing the low temperature solvent fractionation and molecular distillation process of the squid oil refined in a general process, the acid value is low, about 40 to 50% of Omega-3 fatty acid (DHA about 24 to 30 %, EPA about 11-16%), heavy metals such as lead (Pb), cadmium (Cd) and arsenic (As), etc. are not detected, reducing serum total cholesterol and serum triglycerides. Therefore, the purified squid oil according to the present invention is excellent in oxidation stability, and even when a relatively small amount is ingested, physiological functionality of the same level or more can be expected. In addition, since the purified squid oil according to the present invention can be easily obtained from squid processed by-products such as squid guts, raw material supply is relatively easy, and high value-added materials using by-products are economical and environmentally friendly.

1 is a process chart showing a manufacturing process of refined squid oil according to the present invention.

Claims (10)

1) extracting, degumming, deoxidizing and decolorizing squid oil from squid gut; 2) separating the low-temperature solvent at -10 to -40 ℃ by adding the bleaching oil prepared in step 1) to the organic solvent; 3) vacuum deodorizing the fractionating oil; 4) molecular distillation of the deodorized oil at 100 ° C. under vacuum for 1 to 2 hours; And 5) A method for producing purified squid oil having a high content of omega-3 fatty acid, comprising the step of adding an adsorbent to the molecular distilled squid oil and filtering the adsorbent. The method of claim 1, wherein the organic solvent in step 2) is one selected from the group consisting of n-hexane, acetone, benzene, ethanol and isopropyl alcohol. The method of claim 1, wherein the organic solvent in step 2) comprises 2 to 4 times (volume) of bleached oil. The method of claim 1, wherein the low temperature solvent fractionation step of step 2) is performed at a vacuum degree of 400 to 500 mmHg for 8 to 16 hours. The method of claim 1, wherein the vacuum deodorizing step of step 3) is performed at a vacuum degree of 2.0 to 4.0 torr and 170 to 190 ° C. for 5 to 6 hours. delete The method of claim 1, wherein the adsorbent in step 5) comprises 2 to 5% by weight of the deodorizing oil. The method of claim 1, wherein the adsorbent in step 5) is at least one selected from the group consisting of MiZuka life F-1G, magnesol (magnesium silicate), acid clay, activated clay, and activated carbon. . A refined squid oil prepared by any one of claims 1 to 8 and containing 40 to 50% of omega-3 fatty acids (DHA and EPA). Health food for improving hyperlipidemia comprising the purified squid oil of claim 9.

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