KR101043596B1 - Composition for Improving Urologic Function Using Extraction of Bokbunja - Google Patents

Abstract

The present invention relates to a composition for improving urinary function using bokbunja extract. More specifically, bokbunja extract and / or vitamin E or L-Arginine added extracts from mature or immature fruits of bokbunja are significantly effective for prostatic hyperplasia, erectile dysfunction and prostatitis.

Oxidative damage caused by ischemia / reperfusion of the bladder is caused by hyperoxide (hypergen peroxide) in prostatic hyperplasia (bladder obstruction), which reduces the contractile force of the bladder smooth muscle and activates the bladder by activating the Rho (Reactive oxygen species) kinase pathway Contracts smooth muscle. However, bokbunja extract and vitamin E are effective in inhibiting the contractile force of bladder muscle and relieving the hypersensitivity of the bladder. In relation to erectile dysfunction, bokbunja extract and L-Arginine inhibit the action of ROS and activate the NO (Nitric oxide) pathway to enhance the relaxation function of corpus cavernosum smooth muscle and maintain endothelial cell integrity in diabetic rats. In addition, chronic prostatitis, especially prostatitis caused by E. coli infection, directly inhibits the antimicrobial action and inflammation to bacteria, and has a function of protecting tissues.

Bokbunja extract of the present invention can be provided as a composition of the drug and health functional food for improving urinary function.

Bokbunja extract, erectile function, urination function, urinary function, enlarged prostate, erectile dysfunction, prostatitis

Description

Composition for Improving Urologic Function Using Extraction of Bokbunja

The present invention relates to a composition for improving urinary function using bokbunja extract. Although Bokbunja has the ability to improve or prevent urinary function (erectile function and urination function), urination disorder, erectile dysfunction and chronic prostatitis, it has been directly assessed using the disease model for bokbunja extract. There was no study. The purpose of the present invention is to present a therapeutic effect on erectile dysfunction, chronic prostatitis, or to enhance the efficiency of urination through a systematic study of them. Efficacy is to provide the composition of therapeutic and health functional foods useful for the human body by elucidating the effect by inducing prostatic hyperplasia, erectile dysfunction, chronic prostatitis in experimental animals. It can also contribute to revitalization of the local economy by creating stable demand for bokbunja growers.

Bokbunja (覆盆子; Rubus coreanus Miquel) is an unripe fruit of the rose family Bokbunja strawberry. It is used for oil wells, dream tablets, and urine. Lighten and blacken hair. It also makes the skin soft and beautiful. As a pharmacological action, anti-inflammatory action, antioxidant action and anti-Helicobacter pylori action have been reported. According to the intention, Bokbunja's efficacy is first to treat men's lack of energy, shortage of semen, vulgaris, and second, to help energy, lighten the body and black hair. Third, to see what is false, to increase sexual function, to warm the inside and to strengthen the energy. Fourth, protect the liver to brighten the eyes. Fifth, to help diuretic and to relieve the lung hyperplasia. Sixth, to treat infertility caused by the wall of the woman's uterus. Seventh, antipyretic drugs are written for colds, recessive diseases, pneumonia, and cough. Bokbunja extracts were extracted from kaempferol, quercetin, ellagitannin, ellagic acid, and sanguiin H-5 stems from flavan-3-ol, proanthocianidin, ellagitannin, and fruit. Despite the lack of research, the fruit is known to contain various phenolic compounds. Bokbunja also contains β-sitosterol. Phenolic compounds are one of the secondary metabolites widely distributed in the plant family and are classified into flavonoids, catechins, and anthocyanins. Recently, studies on the physiological activity of these components have been made in various angles, antioxidant activity, anti-aging, hyperlipidemia inhibition, antibacterial, anti-fungal and anti-tumor action has been reported.

Recent studies on bokbunja have been shown to be effective against antioxidants, anti-Helicobacter pylori, anti-cancer, anti-inflammatory and sexual function. 1) excellent therapeutic and prophylactic effects on degenerative inflammatory diseases, stroke caused by stroke, dementia, gout and arthritis; The best anti-inflammatory effect among the immature, ripened, leaves and seeds of bokbunja is a leaf extract that inhibits neurotoxins. 2) antioxidant effect was 2.5 times higher than vitamin C; It also shows high effects in immature fruit, mature fruit, root and leaf, and anthocyanin has antioxidant effect. 3) bacterial inhibition, virus inhibition; Helicobacter pylori, intestinal bacteria, E. coli is effective. 4) anticancer activity; It is also effective in destroying cancer cells, roots and branches from immature fruit and leaf extract. 5) sexual function related; Testosterone increases approximately 16-fold after 5 weeks of nectar administration. 6) antihypertensive action; Polyphenol compounds isolated from bokbunja strain inhibit the secretion of catecholamines in the adrenal medulla via nitric oxide pathway. 7) pain suppression and anti-inflammatory effects, and 8) anti-allergic effects have been reported. However, Bokbunja has many functions that can improve or prevent urination disorder, erectile dysfunction, and chronic prostatitis, but it is effective to improve the effectiveness and urination efficiency of erectile dysfunction and chronic prostatitis. Since no studies have directly assessed the effects of disease models, studies on this are needed.

The prior art regarding the medical use of bokbunja extract is Korean Patent Registration 10-0585487 (anti-helicobacter composition comprising bokbunja extract) is dibutyl phthalate or 4- (4-hydroxyphenyl) -2- extracted with an organic solvent It is an anti-helicobacter composition comprising butanone as an active ingredient. Korean Patent Registration 10-0616067 (an anticancer composition comprising bokbunja extract) relates to an anticancer composition using an extract extracted from bokbunja with hot water and an organic solvent.

The bokbunja extract and / or vitamin E or L-Arginine added in bokbunja mature and immature fruit of the present invention is significantly effective in prostatic hyperplasia, erectile dysfunction and prostatitis. Therefore, the bokbunja of the present invention is effective in improving the erection and urination function, and to investigate the effect of bokbunja extract on the experimental animals of the disease model of prostate hyperplasia and erectile dysfunction. In addition, this study examines the complex therapeutic effects of bokbunja and other therapeutic agents, establishes its value as an additional therapeutic agent and diversifies its range of applications.

Although the present invention has many functions to improve or prevent urinary disorders or erectile dysfunction, the present invention does not have a systematic study on the quality of the extracts of Bokbunja or a direct evaluation using actual disease models. Presenting the results, it is to develop a therapeutic or health food.

The present invention is to determine whether the bokbunja extract is effective in prostatic hypertrophy 1) the effect of bokbunja extract in the bladder smooth muscle oxidative damage model in vitro, 2) the detrusor of bladder extract in bladder outlet complete occlusion model (acute urinary tract model) Smooth muscle), and 3) animal experiments on the detrusor muscle (bladder smooth muscle) of bokbunja extract in bladder outlet complete occlusion model (chronic urinary tract model). In addition, 1) the effect of Bokbunja extract on oxidative damage model of penile cavernous smooth muscle in vitro, 2) the effect of Bokbunja extract on erectile tissue in insulin-dependent diabetes model rats, and 3) Bokbunja in aging rats. Animal testing is performed on the effects of extracts on erectile tissues. And to find out whether it is effective in chronic prostatitis 1) The antimicrobial effect of bokbunja extract against chronic prostatitis-causing bacteria, 2) the effect of bokbunja extract in autoimmune prostatitis model, and 3) the effect of bokbunja extract in chronic bacterial prostatitis model.

The present invention has a significant effect on prostatic hyperplasia, erectile dysfunction and chronic prostatitis in the bokbunja extract and / or vitamin E or L-Arginine added from the mature and immature fruit of bokbunja.

The present invention shows a composition for improving the urinary function of the erection function and / or urination function using the bokbunja extract.

The erectile function in the above can be used by mixing the bokbunja extract with a pharmaceutically acceptable excipient for improving erectile dysfunction.

The erectile function in the above can be used by mixing the bokbunja extract with pharmaceutically acceptable excipients in the effective concentration range for improving erectile dysfunction.

L-arginine or vitamin E and pharmaceutically acceptable excipients may be used in bokbunja extract for improving erectile dysfunction.

L-arginine or vitamin E in bokbunja extract for improving the erectile dysfunction can be used in combination with a pharmaceutically acceptable excipient in the effective concentration range.

The urination function in the above can be used by mixing the bokbunja extract with a pharmaceutically acceptable excipient for improving the prostate hypertrophy.

The urination function in the above can be used by mixing the bokbunja extract with pharmaceutically acceptable excipients in the effective concentration range for improving the prostate hypertrophy.

The urination function in the above can be used by mixing the bokbunja extract with a pharmaceutically acceptable excipient for the prevention of prostatitis.

The urination function in the above can be used in combination with a pharmaceutically acceptable excipient in the effective concentration range of bokbunja extract for the prevention of prostatitis.

The bokbunja extract has a high antimicrobial activity against any one or more prostatitis bacteria selected from the group of B. subtilis, E. coli, K. pneumoniae, S. marcescens, E. faecalis, S. aureus and C. albicans .

The present invention shows a health functional food composition for improving the urinary function of the erection function and / or urination function using the bokbunja extract.

The present invention represents a health functional food composition for improving the urinary function of the erection function and / or urination function comprising the bokbunja extract.

In the above erectile function is used for mixing the bokbunja extract with a food acceptable excipient for improving the erectile dysfunction indicates a food composition for improving the erectile function.

In the above erectile function is a food composition for improving the erectile function by using the bokbunja extract mixed with food excipients in the effective concentration range for erectile dysfunction improvement.

In the above urination function refers to a food composition for improving urination function using bokbunja extract mixed with a food acceptable excipient for improving the prostate enlargement.

The urination function refers to a food composition for improving urination function using bokbunja extract in combination with a food acceptable excipient in the effective concentration range for improving the prostate enlargement.

Bokbunja extract in the present invention can be prepared by the following method using the bokbunja of mature and / or immature fruit in Gochang-gun, Jeollabuk-do.

1) Grind 1kg of Bokbunja pulp using a grinder.

2) Put the pulverized bokbunja pulp in 3kg of ethanol and stirred at room temperature for 5 hours.

3) After standing at room temperature for one day, it is filtered to obtain bokbunja ethanol extract.

4) Evaporate and dry ethanol.

5) The obtained extract is dried in vacuo.

In the present invention, the above pharmaceutically acceptable excipients and / or food acceptable excipients may be carried out by those skilled in the art with appropriate choice, and thus the details thereof will be omitted. .

Hereinafter, the content of the present invention will be described in detail through examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

<Example 1>; Enlarged prostate

Example 1-1 Effect of Bokbunja Extract in Vitro Bladder Smooth Muscle Oxidative Damage Model

(A) Preparation of bladder tissue sections

Mice were inoculated by injecting 100% carbon dioxide into a sealed container for 30 seconds to 1 minute, and then immediately induced death by carotid artery dissection. Separating and government (dome) side of the excised paper bladder, in a 100% physiological solution of saturated HEPES buffer with oxygen (NaCl 140mM, KCl 5mM, CaCl 2 2mM, MgCl 2 1mM, glucose 11mM, HEPES 5mM pH 7.4) Bladder smooth muscle sections were made 1 × 1 × 4 mm in size.

(B) evaluation of tissue sections

The bladder smooth muscle section of rat obtained in (A) was transferred to an experimental container with a capacity of 15 ml, and then connected to an isometric force transducer 52-9545, Harvard, UK, and the resulting signal was converted into a digital-analog signal converter and amplifier. Records were stored on a personal PC (IBM-PC, Pentium 4) using (PowerLab 4SP, ADI instruments, Australia). As experimental conditions, a mixed gas (95% by volume of oxygen and 5% by volume of carbon dioxide) was supplied at a constant temperature of 37 ° C. under a stable tension of 0.5 g. In order to recover from injury and to avoid the accumulation of metabolites, the contractile force was measured after resting for more than 2 hours while changing the physiological solution every 30 minutes. The physiological solution used in the experiment was formulated to be NaCl 116mM, KCl 5mM, CaCl ₂ 2mM, MgCl ₂ 1mM, NaHCO ₃ 24mM, glucose 11mM, pH was adjusted to 7.4 using HCl. After rest, administration of appropriate dose of acetylcholine (ACh) 10 ^-5 M to confirm the condition of the tissue sections after rest, and after ACh administration to evaluate the appropriate maintenance of contraction by ACh Observation was made for 30 minutes without special treatment, and the appropriate tension was considered to be maintained when the initial value of the maximum stable contractile tension was expressed at 90% or more after 30 minutes. After contraction, it was confirmed whether relaxation by sodium nitroprusside (SNP) 10 ^-5 M was induced, and tissues that did not exhibit proper contraction and relaxation response were excluded. In all experiments, the condition of the tissues was verified. When less than 50% of the initial contraction and relaxation reactions were observed, the tissues were considered damaged and the experiment was terminated.

(1) Effect of Hydrogen peroxide (HP) on the contraction of bladder

HP inhibits contraction caused by ACh when exposed for a long time, and bokbunja extract partially recovers it. As shown in FIG. 1, HP expresses bladder smooth muscle in a concentration-dependent manner (3 × 10 ^-6 , 3 × 10 ^-5 , 3 × 10). ^-4 , 3 × 10 ^-3 , 3 × 10 ^-2 g%) Shrink.

(2) confirmation of contraction mechanism of bladder smooth muscle by HP and the effect of Bokbunja extract, vitamin E; Effects of Bokbunja Extract and Vitamin E on Bladder Smooth Muscle Contraction by HP;

Pretreatment with vitamin E 10 ^-5 M and bokbunja extract 0.05mg / ml showed HP concentration-dependent (3 × 10 ^-6 , 3 × 10 ^-5 , 3 × 10 ^-4 , 3 × 10 ^-3 , 3 × 10 ^-2 g%) Contraction was significantly suppressed. The present invention confirms that the contractile force of bladder smooth muscle is reduced when prolonged treatment with hydrogen peroxide (HP), which is one of the representative reactive oxygen species (ROS), is reduced partially by bokbunja extract. Was observed. A new finding, which differs from previous studies of bladder smooth muscle, is that HP has increased contractile force of bladder smooth muscle. HP itself contracts bladder smooth muscle. It also enhances the contraction of bladder smooth muscle by PE, an alpha 1 adrenoceptor agonist. Alpha 1 adrenoceptor is a representative pathway involved in bladder hypersensitivity, and blocking agents are representative agents in the treatment of prostatic hyperplasia.

As described above, HP reduces the contractile force of bladder smooth muscle and increases the sensitivity of bladder smooth muscle by activating the Rho kinase pathway. The extract of Bokbunja and vitamin E inhibits the decrease of contractile force and increased sensitivity of bladder muscle by HP.

The bokbunja extract in the bokbunja pulp pulverized using a grinder in 3kg of ethanol and stirred at room temperature for 5 hours, and then left at room temperature for one day, filtered to obtain bokbunja ethanol extract and then ethanol evaporated to dryness and vacuum dried Bokbunja extract was used.

<Example 1-2> Effect of bokbunja extract on detrusor muscle (bladder smooth muscle) in bladder outlet complete occlusion model (acute urinary tract model)

A) Bladder outlet complete occlusion model (acute urinary tract model) and control (sham operation) production

(1) Experimental animal: Sprague-Dawley Rat (SD rat)

(2) model making method

Before taking the mouse immediately, take bokbunja for 48 hours, and then anesthetize by intramuscular injection of ketamine (40mg / kg) and xylazine (20mg / kg). The urethra was ligated to cause complete occlusion, and then the abdominal incision site was closed. The control group was closed after anesthesia without urethral ligation. After 8 hours, the animals were immediately killed to visually confirm that the bladder was completely obstructed, and then the bladder tissue sections were prepared in the same manner as in Example 1-1, and the tissue sections were evaluated.

(3) how to take bokbunja

0.2g / day bokbunja extract per rat was prepared as a solution and supplied instead of water, and the control group was given tap water.

B) Evaluation of contractile force changes in acute urinary bladder smooth muscle with or without bokbunja

1) Observation of the effect of PE on the contraction of bladder

Concentration-dependent contractile response changes by PE with and without the use of bokbunja in the control and experimental groups were observed. After acute urinary tract, bladder smooth muscle contractility increased significantly. 3) recovered. Prostatic hyperplasia is caused by obstruction of urinary bladder due to the enlarged prostate gland, causing the bladder muscle to thicken and become sensitive for urinary discharge. The incidence of acute urinary retention, one of the serious complications of enlarged prostate, has been reported to be 3 to 5% in five years. In the present invention, when the contractile force of bladder muscle was measured in the acute urinary tract model in which the bladder outlet was completely occluded for about 8 hours, the contractile force by PE was increased, and the increase in the contractile force was restored to the same level as that of the normal control group in the group taking Bokbunja extract. It became. Alpha-adrenergic stimulation by PE is not very dominant in normal bladder, but in pathological conditions the receptor density can increase and the norepinephrine-induced response can change from relaxation to contraction. Therefore, the increase of the receptor density in acute urinary tract may explain the increase in the contraction response by PE. The result of the recovery of the contraction to the control level when taking bokbunja is the effect of bokbunja extract on the bladder hypersensitivity in previous ex vivo experiments. It is consistent with the result shown.

Prostatic hyperplasia is a disease that causes urinary tract disorders and irritable bladder due to bladder outlet obstruction.

<Example 1-3> Effects of Bokbunja Extracts on the Detrusor Muscle (bladder smooth muscle) in the complete bladder outlet model (chronic urinary tract model)

A) Bladder outlet complete occlusion model (chronic urinary tract model) and control production

(1) Experimental animal: Sprague-Dawley Rat (SD rat)

(2) model making method

The rats were anesthetized by intramuscular injection of ketamine (40mg / kg) and xylazine (20mg / kg), followed by an incision in the lower abdomen in the supine position to exfoliate the urethra, and a polyethylene conduit was placed in the periphery of the urethra. Ligation and removal of the polyethylene conduit resulted in partial occlusion and the abdominal incision site was closed. The control group was closed after anesthesia without urethral ligation. After dividing the experimental group into two groups and taking the bokbunja in only one group, two weeks later the rats were killed immediately to make bladder tissue sections in the same manner as in Example 1-1, and evaluated the tissue sections.

(3) how to take bokbunja

0.2g / day bokbunja extract per rat was prepared as a solution and supplied instead of water, and the control group was given tap water.

B) Evaluation of contractile force changes in chronic urinary bladder smooth muscle with or without bokbunja

Changes in Contractility of Chronic Urinary Bladder Smooth Muscle with or without Bokbunja

(1) Effect on the contraction of bladder by PE

After chronic urinary tract, the contractile force of bladder smooth muscle did not change with PE, but the contractile force increased significantly in the group taking bokbunja.

(2) Observation of the effect of ATP on the contraction of bladder

After chronic urinary tract, the contractile force of bladder smooth muscle did not change for ATP, but the contractile force increased significantly in the group taking bokbunja.

<Example 2>; Erectile Dysfunction

<Example 2-1>; Effect of Bokbunja Extract on In Vitro Penile Cavernous Smooth Muscle Oxidative Damage Model

(1) Effects of Bokbunja Extracts on Shrinkage Relaxation of Penile Cavernous Erectile Tissues in Rats and Rabbits;

(A) Bokbunja extract and vitamin E observed the relaxation effect by acetylcholine (ACh) (Fig. 4); ACh 10 ^-8 ~10 ^-4 M concentration dependent relaxation of smooth muscle, has been enhanced to 0.05 mg / ml bokbunja extract was 20 minutes before scoring and vitamin E pretreatment.

(B) Observation of the mechanism of relaxation of rabbit penile corpus cavernosum smooth muscle by bokbunja extract (Fig. 5); After observation of nitric oxide synthase, L-arginine 10 ^-5 M pretreatment increased relaxation by ACh and combined L-arginine 10 ^-5 M + bokbunja extract (0.05 mg / ml pretreatment) The treatment enhances the relaxation effect of L-arginine as shown in FIG. 5.

(C) Observation of the effect of hydrogen peroxide (HP) on contraction and relaxation of penile cavernous smooth muscle in rats and rabbits

The effect of HP on the contraction of corpus cavernosum smooth muscle and the effect of Bokbunja extract on PE 10 ^-5 M was found to be HP concentration dependent (3 × 10 ^-6 , 3 × 10 ^-5 , 3 × 10 ^-4 , 3 × 10 ^-3 , 3 × 10 ^-2 g%) It showed an effect of reducing contractile force. This contraction force reduction was partially recovered by the bokbunja extract treatment as shown in FIG. Observing the effect of Bokbunja extract on penile cavernous smooth muscle relaxation effect by HP, after the pre-treatment of Bokbunja extract 0.05mg / ml, the relaxation effect of the cavernous smooth muscle muscle for ACh reduced by HP was recovered as shown in FIG.

The present invention observed that bokbunja extract and vitamin E enhance the relaxation response of the corpus cavernosum smooth muscle through the process of strengthening the endothelial function. Bokbunja extract was found to increase L-arginine's ACh-relaxation potentiating effect. It also recovers the relaxation response by ACh reduced by HP. In view of the fact that L-arginine is a substrate of NOS, at least the bokbunja extract is finally activated by activating NO production process, reducing arginase activity by reducing or reducing free radicals, or inhibiting NO oxidation. It is estimated to maintain NO concentration of cavernous smooth muscle. The bokbunja pre-extract of the present invention may be provided as a food or medicine effective in preventing erectile dysfunction caused by various diseases causing vascular diseases.

<Example 2-1>; Effect of Bokbunja Extract on Erectile Tissue in Insulin-Dependent Diabetic Rats

The effect of ACh on the relaxation of penile cavernous smooth muscle in the changes in the contractile force of the corpus cavernosum smooth muscle in diabetic rats with or without bokbunja was observed (Fig. 8), after contraction with PE 10 ^-5 M The corpus cavernosum relaxation response was significantly increased at concentrations above 10 ^-7 M in the bokbunja group.

After taking the bokbunja extract of the present invention, the relaxation response of the corpus cavernosum smooth muscle to ACh was significantly increased in the diabetic rats, and it was confirmed that the relaxation of the penile smooth muscle reduced by diabetes was recovered by the use of bokbunja.

<Example 3>; Chronic prostatitis

<Example 3-1>; Antibacterial Effect of Bokbunja Extract against Chronic Prostatitis

(1) Antimicrobial effect of 1% Bokbunja extract: K.pneumoniae, P.mirabilis, and C.albicans did not show any difference between the control and the number of colonies. B.subtilis, E.coli, S.marcescens, E.faecalis, S .aureus showed high antimicrobial effect over time.

(2) Antimicrobial effect of 2% Bokbunja extract: P.mirabilis and S.marcescens showed no difference between control group and B. subtilis, E.coli, K.pneumoniae, E.faecalis, S.aureus, C .albicans showed a high antimicrobial effect over time (Fig. 9). Bokbunja extract of the present invention has the effect of inhibiting prostatitis causing bacteria.

<Example 3-2>; Effect of Bokbunja Extract on Autoimmune Prostatitis Model

Autoimmune Prostatitis Model In order to confirm the histological findings (HE staining) according to the use of bokbunja in rats, the prostate gland was extracted and classified according to the histological prostatitis classification of Nickel et al. (2001). There was extensive individual inflammatory cell infiltration in at least 50% of the tissue, both tissue and stromal tissue. The degree of inflammation was mild and Gr 1 (mild) prostatitis was observed (× 200), and the apoptosis index (4.8) of the prostatitis group taking bokbunja was lower than that of the group without the bokbunja (10.4) (Fig. 10). It became. (p <0.01) The Ki-67 index, which showed proliferative responses, also showed a slight difference of 0.17 in the group without bokbunja and 0.25 in the group.

<Example 3-3>; Effect of Bokbunja Extract on Chronic Bacterial Prostatitis Model

(1) Optical microscopic findings after HE staining

When the prostate gland of the chronic bacterial prostatitis model injected into mice by injecting E. coli, the most common cause of prostatitis, was extracted according to the histological prostatitis classification of Nickel et al. (2001), the prostatitis group had more than moderate inflammation. Although observed (FIG. 11, × 200), most of these inflammatory findings were recovered in the bokbunja group and returned to the normal control level (see Table 1).

Table 1. Prostate Inflammation Effects of Bokbunja

division Control group Prostatitis-Bokbunja Prostatitis Scope of tissue infiltration of inflammatory cells Organization none none multifocal Peripheral Organization focal multifocal multifocal Stromal tissue none none multifocal Degree of inflammation (density of inflammatory cells) Organization none none mild (<100) Peripheral Organization mild (<100) mild (<100) mild (<100) Stromal tissue mild (<100) none severe (> 500) apoptosis inde × 0.6 One 1.4 Ki-67 inde × 1.75 5.5 1.5

(2) Ki-67 Immunohistochemical Staining

Ki-67 index was 1.75 in the control group, 1.5 in the prostatitis group and 5.5 in the prostatitis-drug group (p <0.05), and the proliferative response (stained part) was increased in the group taking the bokbunja (Fig. 12). , X 200) (see Table 1).

Bokbunja of the present invention is expected to be developed as a prophylactic and therapeutic agent for bacterial prostatitis by directly inhibiting inflammation and protecting against inflammation in prostatitis caused by chronic bacterial prostatitis, especially Escherichia coli.

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated that it can be changed.

The bokbunja extract of the present invention suppresses the decrease in contractility and hypersensitivity of the bladder muscle due to enlarged prostate, and is particularly useful for the prevention and treatment of irritable bladder symptoms after acute urinary tract, which is a serious complication of prostatic hyperplasia. In erectile dysfunction, diabetic rats have reduced penile smooth muscle relaxation by diabetic administration, and bokbunja extract has the effect of improving or inhibiting erectile function in diabetic erectile dysfunction patients. In addition, it is expected to be industrially useful as a prophylactic and therapeutic agent for bacterial prostatitis by directly inhibiting inflammation and protecting against inflammation in prostatitis caused by chronic bacterial prostatitis, especially E. coli infection.

Figure 1 shows the effect of HP (hydrogen peroxide) on the contraction of the bladder.

2 is the effect of bokbunja extract and vitamin E on the bladder smooth muscle contraction response by HP.

3 is an effect on the contractile response of the bladder by PE after acute urinary tract.

Figure 4 is the effect of bokbunja extract and vitamin E in the corpus cavernosum smooth muscle in vitro.

5 is a mechanism observation of the relaxation effect of the rabbit corpus cavernosum smooth muscle by the bokbunja extract.

6 is an observation of the effect of HP on the contraction of the corpus cavernosum smooth muscle and the effect of bokbunja extract.

Figure 7 is an observation of the effect of HP on the relaxation of the corpus cavernosum smooth muscle and the effect of bokbunja extract.

8 is an observation of the effect of bokbunja extract on the relaxation response of the corpus cavernosum smooth muscle muscle by ACh in insulin-dependent diabetes model rats.

Figure 9 is the antimicrobial effect of 2% Bokbunja extract against chronic prostatitis causative bacteria.

Figure 10 shows the effect of the bokbunja extract in the autoimmune prostatitis model, Figure 10a shows the bokbunja unadministered group, Figure 10b shows the bokbunja taking group.

Figure 11 shows the effect of bokbunja extract in chronic bacterial prostatitis model, Figure 11a is a control group, Figure 11b is a prostatitis group, Figure 11c shows a bokbunja taking group of the prostatitis model.

12 shows Ki-67 immunohistochemical staining to see the effects of bokbunja extract in the chronic bacterial prostatitis model, FIG. It is shown.

Claims (9)

B. subtilis, E.coli, K.pneumoniae, S.marcescens, E.faecalis, S.aureus and C. albicans In addition to E, a pharmaceutically acceptable excipient may be included to improve erectile dysfunction, improve prostatic hyperplasia, or prevent prostatitis. After leaving for one day at room temperature filtered to obtain a bokbunja ethanol extract and then evaporated to dry ethanol and then dried under vacuum drying composition for urinary function of erectile function and urination function. delete delete delete delete delete B. subtilis, E.coli, K.pneumoniae, S.marcescens, E.faecalis, S.aureus and C. albicans In addition to E, it has an effect of improving erectile dysfunction, improving prostatic hyperplasia, or preventing prostatitis by including a food acceptable excipient, and the bokbunja extract is mixed with 1kg of pulverized bokbunja pulp in 3kg of ethanol and stirred at room temperature for 5 hours. After leaving for one day at room temperature filtered to obtain the bokbunja ethanol extract and then evaporated to dry ethanol and then dried in a vacuum dried health food composition for improving the urinary function of erectile function and urination function. delete delete

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