KR101012555B1 - Method for preparing the functional fermented jujube and it's product - Google Patents
Method for preparing the functional fermented jujube and it's product Download PDFInfo
- Publication number
- KR101012555B1 KR101012555B1 KR1020080050121A KR20080050121A KR101012555B1 KR 101012555 B1 KR101012555 B1 KR 101012555B1 KR 1020080050121 A KR1020080050121 A KR 1020080050121A KR 20080050121 A KR20080050121 A KR 20080050121A KR 101012555 B1 KR101012555 B1 KR 101012555B1
- Authority
- KR
- South Korea
- Prior art keywords
- jujube
- herbal
- yeast
- mixed
- lactic acid
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 36
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 241001247821 Ziziphus Species 0.000 claims abstract description 150
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 92
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 48
- 241000894006 Bacteria Species 0.000 claims abstract description 46
- 239000004310 lactic acid Substances 0.000 claims abstract description 46
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 46
- 239000002609 medium Substances 0.000 claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 33
- 230000008569 process Effects 0.000 claims abstract description 28
- 241000208340 Araliaceae Species 0.000 claims abstract description 23
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 23
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 23
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 23
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 23
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 23
- 239000011425 bamboo Substances 0.000 claims abstract description 23
- 235000008434 ginseng Nutrition 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 claims abstract description 20
- 230000001954 sterilising effect Effects 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 235000013871 bee wax Nutrition 0.000 claims abstract description 14
- 239000012166 beeswax Substances 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 244000104275 Phoenix dactylifera Species 0.000 claims abstract description 10
- 235000010659 Phoenix dactylifera Nutrition 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 244000082204 Phyllostachys viridis Species 0.000 claims abstract 9
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 42
- 239000000284 extract Substances 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 4
- 244000163122 Curcuma domestica Species 0.000 claims description 3
- 235000003392 Curcuma domestica Nutrition 0.000 claims description 3
- 241000257303 Hymenoptera Species 0.000 claims description 3
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 206010052428 Wound Diseases 0.000 claims description 3
- 208000027418 Wounds and injury Diseases 0.000 claims description 3
- 235000003373 curcuma longa Nutrition 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 235000013976 turmeric Nutrition 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 2
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 2
- 241000235072 Saccharomyces bayanus Species 0.000 claims description 2
- 241000877401 Saccharomyces ellipsoideus Species 0.000 claims description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 244000126002 Ziziphus vulgaris Species 0.000 claims 3
- 241001668545 Pascopyrum Species 0.000 claims 1
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 15
- 208000031226 Hyperlipidaemia Diseases 0.000 abstract description 8
- 230000002378 acidificating effect Effects 0.000 abstract description 6
- 230000000144 pharmacologic effect Effects 0.000 abstract description 6
- 241000411851 herbal medicine Species 0.000 abstract description 5
- 238000005406 washing Methods 0.000 abstract description 5
- 238000003809 water extraction Methods 0.000 abstract description 5
- 208000030159 metabolic disease Diseases 0.000 abstract description 3
- 239000008280 blood Substances 0.000 description 24
- 210000004369 blood Anatomy 0.000 description 24
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 241001330002 Bambuseae Species 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 239000008103 glucose Substances 0.000 description 13
- 235000009200 high fat diet Nutrition 0.000 description 13
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 11
- 150000003626 triacylglycerols Chemical class 0.000 description 11
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 8
- 235000012000 cholesterol Nutrition 0.000 description 6
- 241000700159 Rattus Species 0.000 description 5
- -1 opened Substances 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 4
- 108010012715 Superoxide dismutase Proteins 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000010030 glucose lowering effect Effects 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical group OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 208000004930 Fatty Liver Diseases 0.000 description 2
- 108010023302 HDL Cholesterol Proteins 0.000 description 2
- 206010019708 Hepatic steatosis Diseases 0.000 description 2
- 238000008214 LDL Cholesterol Methods 0.000 description 2
- PCZOHLXUXFIOCF-UHFFFAOYSA-N Monacolin X Natural products C12C(OC(=O)C(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 PCZOHLXUXFIOCF-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 208000010706 fatty liver disease Diseases 0.000 description 2
- 235000012907 honey Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- PCZOHLXUXFIOCF-BXMDZJJMSA-N lovastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)[C@@H](C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 PCZOHLXUXFIOCF-BXMDZJJMSA-N 0.000 description 2
- 229960004844 lovastatin Drugs 0.000 description 2
- QLJODMDSTUBWDW-UHFFFAOYSA-N lovastatin hydroxy acid Natural products C1=CC(C)C(CCC(O)CC(O)CC(O)=O)C2C(OC(=O)C(C)CC)CC(C)C=C21 QLJODMDSTUBWDW-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 231100000240 steatosis hepatitis Toxicity 0.000 description 2
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 2
- 229960001052 streptozocin Drugs 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000010632 SOD assay Methods 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- 229930003270 Vitamin B Chemical group 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 229960002632 acarbose Drugs 0.000 description 1
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 description 1
- YBCVMFKXIKNREZ-UHFFFAOYSA-N acoh acetic acid Chemical compound CC(O)=O.CC(O)=O YBCVMFKXIKNREZ-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000002402 anti-lipaemic effect Effects 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 239000012676 herbal extract Substances 0.000 description 1
- 229940126904 hypoglycaemic agent Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002350 laparotomy Methods 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229940124595 oriental medicine Drugs 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Chemical group 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
본 발명은 대추에 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 열수추출한 한방배지를 혼합하고 멸균한 후 유산균과 효모가 혼합된 배양액을 접종하고 발효한 다음 살균 및 건조함으로써,The present invention by mixing and sterilizing the jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax hot water extracted herbal medium and inoculated with a culture solution mixed with lactic acid bacteria and yeast and then sterilized and dried ,
한약재와 유산균 및 효모의 약리적 성분을 대추에 함유시켜 대추에 부족한 약리적 기능성을 향상시킴에 따라 당뇨, 고지혈증과 같은 대사성질환을 개선할 수 있을 뿐만 아니라 대추의 수요를 증가시켜 대추농가의 수익창출을 증대할 수 있다.By adding pharmacological components of Chinese herbal medicine, lactic acid bacteria and yeast to jujube, it improves the pharmacological functions lacking in jujube, and not only improves metabolic diseases such as diabetes and hyperlipidemia, but also increases jujube farm's profit generation by increasing the demand of jujube. can do.
본 발명에 따라 기능성 발효 대추제조방법은. Functional fermented jujube production method according to the present invention.
대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀 중 흠집이나 상처가 나지 않은 대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 선별한 후 약산성액에 침지시키고 흐르는 물에 세척하는 원재료의 선별 및 세척공정(제1공정)과; 상기 제1공정에서 선별 후 세척된 대추열매의 씨를 열매의 씨 제거기를 이용하여 제거하는 대추열매의 씨 제거공정(제2공정)과; 상기 제1공정에서 선별 후 세척된 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀를 혼합하고, 증류수를 혼합한 후 열수추출하는 한방배지 제조공정(제3공정)과; 상기 제2공정에서 씨가 제거된 대추열매와 상기 제3공정에서 제조된 한방배지를 혼합한 후 멸균기에서 멸균하는 혼합 및 멸균공정(제4공정)과; 액체배지에 유산균과 효모를 혼합접종한 후 진탕배양기를 이용하여 배양하는 유산균 및 효모 배양공정(제5공정)과; 상기 제4공정에서 대추와 한방배지가 혼합 및 멸균된 혼합물에 상기 제5 공정에서 유산균과 효모가 배양된 배양액을 혼합하여 접종시킨 후 발효기에서 발효하는 접종 및 발효공정(제6공정)과; 상기 제6공정의 기능성 한방대추를 건조기에서 건조하여 살균 및 건조하는 살균 및 건조공정(제7공정)으로 이루어진 것을 특징으로 한다.Jujube fruit, jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, unbroken jujube fruit, jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, bee And then sorting and washing the raw material (first step), which is immersed in a weak acidic liquid and washed in running water; A seed removal step of the date palm fruit (second step) for removing the seeds of the date palm washed after the screening in the first step using a fruit seed remover; Herbal medium manufacturing process (third step) of mixing the jujube leaves, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax and then distilled water and hot water extraction after screening in the first step; A mixing and sterilizing step (fourth step) of mixing the jujube fruit from which seed was removed in the second step and the herbal medium prepared in the third step and then sterilizing in a sterilizer; Lactic acid bacteria and yeast culture step (Fifth step) of incubating the mixed medium and lactic acid bacteria and yeast in a shaker culture medium; An inoculation and fermentation step (sixth step) in which the jujube and herbal medium are mixed and sterilized in the fourth step and inoculated by mixing the culture medium in which the lactic acid bacteria and yeast are cultured in the fifth step and then fermented in a fermenter; The functional herbal jujube of the sixth process is characterized in that it consists of sterilization and drying process (seventh process) to sterilize and dry by drying in a dryer.
발효대추, 대추, 한방배지, 유산균, 효모, 발효, 살균, 건조 Fermented jujube, jujube, herbal medium, lactic acid bacteria, yeast, fermentation, sterilization, drying
Description
본 발명은 대추에 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 열수추출한 한방배지를 혼합하고 멸균한 후 유산균과 효모가 혼합된 배양액을 접종하고 발효한 다음 살균 및 건조함으로써, The present invention by mixing and sterilizing the jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax hot water extracted herbal medium and inoculated with a culture solution mixed with lactic acid bacteria and yeast and then sterilized and dried ,
한약재와 유산균 및 효모의 약리적 성분을 대추에 함유시켜 대추에 부족한 약리적 기능성을 향상시킴에 따라 당뇨, 고지혈증과 같은 대사성질환을 개선할 수 있을 뿐만 아니라 대추의 수요를 증가시켜 대추농가의 수익창출을 증대할 수 있다.By adding pharmacological components of Chinese herbal medicine, lactic acid bacteria and yeast to jujube, it improves the pharmacological functions lacking in jujube, and not only improves metabolic diseases such as diabetes and hyperlipidemia, but also increases jujube farm's profit generation by increasing the demand of jujube. can do.
대추는 우리나라 기후풍토에 적합하여 재배가 용이하고 생산물의 저장성이 좋아서 널리 재배된 과수 중에 하나이다. 대추는 단백질, 지방 등의 영양소와 36종의 다양한 무기원소가 함유되어 있으며, 특히 비타민 C, 비타민 B군, 칼슘, 인 등의 영양분이 천연 비타민제라고 할 정도로 많이 함유되어 있다. 또한 비교적 수분이 낮아서 풋대추의 수분함량은 74%이고, 붉은 대추 생것의 수분함량은 60%이며, 건조대추의 수분함량은 30%이다. 대추의 주성분인 포도당과 과당의 함량은 28%이고, 단백질의 함량은 1.5%이다. Jujube is one of the most widely grown fruits because it is suitable for the climate of Korea and its cultivation is easy. Jujube contains protein, fat and other nutrients, and 36 kinds of various inorganic elements. In particular, jujube contains vitamin C, vitamin B group, calcium, phosphorus and so on. In addition, the moisture content of green jujube is 74%, the red jujube raw water content is 60%, and the dry jujube water content is 30%. The major components of jujube are 28% glucose and fructose, and 1.5% protein.
한방에서는 대추를 완화강장제로 사용하고, 잘 익은 대추를 증숙 및 건조시켜 달여 먹으면 열을 내리게 하고, 변을 묽게 하여 변비를 없애며, 기침도 멎게 하는 것으로 알려져 있다.In oriental medicine, jujube is used as a relaxation tonic, steamed and dried ripe jujube, and it is known to heat down when eaten, to lower constipation, to remove constipation, and to cough.
최근에는 인체의 면역력을 높이는 물질(사이클릭 에엠피, Cyclic AMP)을 활성화시키는 성분이 대추에 많이 함유되어 있다는 연구 보고가 있고, 대추를 에탄올에 추출한 추출액을 간이 손상된 쥐에 투여하여 실험한 결과 손상된 간의 회복 및 보호효과가 좋은 것으로 보고된 바 있다.Recently, there have been reports that jujube contains a lot of ingredients that activate the body's immunity (Cyclic AMP), and the jujube extract was extracted from ethanol to rats with liver damage. Liver recovery and protection have been reported to be good.
그러나, 대추의 생산량은 최근 5년간에 1994년에 12,600톤에서 1996년도에 14,000톤 정도로 최고에 이르렀다가 계속 감소 되는 추세이다. 종래에 국내산 대추의 대부분은 한약재나 제수용품으로만 주로 소비되어 왔으나, 1994년부터 새로운 대추음료의 개발로 수요가 늘어 대추의 생산량도 증대하였다. 그러나, 이러한 대추음료의 소비가 둔화되면서 대추 가공량이 현저히 감소되어, 대추 재배 농가의 소득이 줄어들고 있는 실정이다.However, jujube production has peaked in the last five years, from 12,600 tonnes in 1994 to 14,000 tonnes in 1996, and then continues to decline. In the past, most of domestic jujube has been mainly consumed only as herbal medicine or dehydration products, but since 1994, demand for jujube has increased due to the development of new jujube drinks. However, as the consumption of jujube beverages slows down, the processing amount of jujube is significantly reduced, and the income of jujube farmers is decreasing.
그리하여 시중에서 생산, 판매되고 있는 대추를 이용하여 가공된 대추 가공제품의 품질개선이 필요할 뿐만 아니라 새로운 제품을 개발이 요구되고 있다.Therefore, not only the quality improvement of processed jujube processed products using jujube produced and sold in the market is required, but also the development of new products is required.
상기와 같은 실정 및 요구를 해결하고자 발명한 것으로,Invented to solve the above situation and needs,
대추에 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 열수추출한 한방배지를 혼합하고 멸균한 후 유산균과 효모가 혼합된 배양액을 접종하고 발효한 다음 살균 및 건조함으로써, By mixing and sterilizing the jujube leaves, jujube seeds, ginseng, bokryeong, jihwang, bamboo leaves, bokbunja, and herbal extracts of hot water extracted from beeswax, inoculate the fermentation broth mixed with lactic acid bacteria and yeast, sterilize and dry,
한약재와 유산균 및 효모의 약리적 성분을 대추에 함유시켜 대추에 부족한 약리적 기능성을 향상시킴에 따라 당뇨, 고지혈증과 같은 대사성질환을 개선할 수 있을 뿐만 아니라 대추의 수요를 증가시켜 대추농가의 수익창출을 증대할 수 있는 기능성 발효 대추 제조방법을 제공하는데 그 목적이 있다. By adding pharmacological components of Chinese herbal medicine, lactic acid bacteria and yeast to jujube, it improves the pharmacological functions lacking in jujube, and not only improves metabolic diseases such as diabetes and hyperlipidemia, but also increases jujube farm's profit generation by increasing the demand of jujube. The purpose is to provide a method for producing a functional fermented jujube.
상기와 같은 목적을 달성하기 위한 본 발명에 따라 기능성 발효 대추 제조방법은, Functional fermented jujube production method according to the present invention for achieving the above object,
대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀 중 흠집이나 상처가 나지않은 대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 선별한 후 약산성액에 침지시키고 흐르는 물에 세척하는 원재료의 선별 및 세척공정(제1공정)과; 상기 제1공정에서 선별 후 세척된 대추열매의 씨를 열매의 씨 제거기를 이용하여 제거하는 대추열매의 씨 제거공정(제2공정)과; 상기 제1공정에서 선별 후 세척된 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀 를 혼합하고, 증류수를 혼합한 후 열수추출하는 한방배지 제조공정(제3공정)과; 상기 제2공정에서 씨가 제거된 대추열매와 상기 제3공정에서 제조된 한방배지를 혼합한 후 멸균기에서 멸균하는 혼합 및 멸균공정(제4공정)과; 액체배지에 유산균과 효모를 혼합접종한 후 진탕배양기를 이용하여 배양하는 유산균 및 효모 배양공정(제5공정)과; 상기 제4공정에서 대추와 한방배지가 혼합 및 멸균된 혼합물에 상기 제5공정에서 유산균과 효모가 배양된 배양액을 혼합하여 접종시킨 후 발효기에서 발효하는 접종 및 발효공정(제6공정)과; 상기 제6공정의 기능성 한방대추를 건조기에서 건조하여 살균 및 건조하는 살균 및 건조공정(제7공정)으로 이루어진 것을 특징으로 한다.Jujube fruit, jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, no scratches or wounds of bees And then sorting and washing the raw material (first step), which is immersed in a weak acidic liquid and washed in running water; A seed removal step of the date palm fruit (second step) for removing the seeds of the date palm washed after the screening in the first step using a fruit seed remover; Herbal medium manufacturing process (third step) of mixing the jujube leaf, jujube seed, ginseng, fueryeong, jihwang, bamboo leaf, bokbunja, beeswax after screening in the first step, hot water extraction after mixing distilled water; A mixing and sterilizing step (fourth step) of mixing the jujube fruit from which seed was removed in the second step and the herbal medium prepared in the third step and then sterilizing in a sterilizer; Lactic acid bacteria and yeast culture step (Fifth step) of incubating the mixed medium and lactic acid bacteria and yeast in a shaker culture medium; An inoculation and fermentation step (sixth step) in which the jujube and herbal medium are mixed and sterilized in the fourth step and inoculated by mixing the culture medium in which the lactic acid bacteria and yeast are cultured in the fifth step and then fermented in a fermenter; The functional herbal jujube of the sixth process is characterized in that it consists of sterilization and drying process (seventh process) to sterilize and dry by drying in a dryer.
본 발명에 의한 기능성 발효 대추 제조방법은, Functional fermented jujube production method according to the present invention,
SOD 유사활성이 높아 항산화효과가 있을 뿐만 아니라 혈중 총콜레스테롤 및 중성지방을 낮추어 고지혈을 예방 및 치료에 효과가 있다.The high SOD-like activity has an antioxidant effect and lowers total cholesterol and triglycerides in the blood to prevent and treat hyperlipidemia.
그리고, 혈당을 낮추어 줌으로서 당뇨를 치료 개선할 수 있다.In addition, diabetes can be cured and improved by lowering blood sugar.
또한, 대추의 기능성 향상에 의한 고부가가치 실현, 대추잎, 씨 등 부산물의 활용성 제고, 기호성, 편의성 제고를 통한 신수요 창출 및 생산성 향상 등을 통한 생산소득 증대 및 지역경제 활성화, 천연물 의약품 신소재 개발 등의 경제적 효과를 제공해준다.In addition, the realization of high value added by improving the functionality of jujube, the increase in the utilization of by-products such as jujube leaves and seeds, the creation of new demands through the improvement of palatability and convenience, the increase of production income and the activation of local economy, the development of new products of natural medicines, etc. It provides economic effects.
이하, 첨부된 도면에 의거 본 발명의 구성을 상세히 설명하면 다음과 같다.Hereinafter, the configuration of the present invention in detail based on the accompanying drawings.
도 1은 본 발명에 따른 기능성 발효 대추 제조방법을 개략적으로 도시한 공정흐름도이다. 1 is a process flow diagram schematically showing a functional fermented jujube manufacturing method according to the present invention.
1. 원재료의 선별 및 세척공정(제1공정)1. Raw material sorting and washing process (1st process)
원재료를 선별한 후 세척하는 공정으로Process of sorting raw materials and washing them
원재료 중 흠집이나 상처가 나지않은 원재료를 선별한 후 약산성액(pH 5~6)에 30~60초간 침지시키고 흐르는 물에 세척한다.Select the raw materials that are not scratched or scratched among the raw materials, and soak them in weak acid solution (pH 5 ~ 6) for 30 ~ 60 seconds and wash them under running water.
이때, 상기 원재료는 대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀(벌꿀)이다.At this time, the raw material is jujube fruit, jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax (honey).
이때, 상기 약산성액은 아세트산(Acetic acid)과 증류수(D.W)를 혼합한 것이다.At this time, the weak acid solution is a mixture of acetic acid (Acetic acid) and distilled water (D.W).
한편, 선별된 원재료를 약산성액에 침지시킨 후 흐르는 물에 세척하는 것은 선별된 원재료에 잔존하는 병원성 미생물을 약산성액을 이용하여 사멸하기 위한 것이며, 약산성액에 침지된 원재료에 잔존하는 약산성액을 제거하기 위해서 위함이다.On the other hand, immersing the selected raw material in the weak acidic liquid and washing in running water is to kill the pathogenic microorganisms remaining in the selected raw material using the weak acidic liquid, and remove the weak acidic liquid remaining in the raw material immersed in the weak acidic liquid. To do that.
2. 대추열매의 씨 제거공정(제2공정)2. Seed removal process of jujube fruit (2nd process)
대추열매의 씨를 제거하는 공정으로,In the process of removing the seeds of jujube,
상기 제1공정에서 선별 후 세척된 대추열매의 씨를 열매의 씨 제거기를 이용하여 제거한다.The seeds of the date palm washed after the selection in the first step are removed using a seed remover of the fruit.
이때, 대추열매의 씨를 제거하기 위해 사용되는 열매의 씨 제거기는 일반적으로 사용되는 열매의 씨 제거기이다.At this time, the seed remover of the fruit used to remove the seeds of jujube fruit is a seed remover of the fruit generally used.
대추열매의 씨를 제거하는 것은 최종생산물의 식감을 향상하기 위함이다.Removing the seeds from the date palm is to improve the texture of the final product.
3. 한방배지 제조공정3. Herbal Medicine Manufacturing Process
대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 혼합하고 증류수를 혼합한 후 열수추출하는 공정으로,Jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax, and distilled water is mixed and hot water extraction process,
상기 제1공정에서 선별 후 세척된 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀를 혼합하고, 증류수를 중량대비 1:20~30으로 혼합한 후 80~100℃에서 2~8시간 열수추출한다.The jujube leaves, jujube seeds, ginseng, bokyeong, jihwang, bamboo leaves, bokbunja, beeswax and mixed with distilled water 1: 1: 30 to 30 by weight after mixing in the first step and then mixed at 80 ~ 100 ℃ 2 ~ 8 Extract hot water from time.
상기 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀를 혼합한 것을 한방혼합물이라 한다.The mixture of jujube leaves, jujube seeds, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, beeswax is called herbal mixture.
이때, 한방혼합물은 11~15중량%의 대추잎, 3~5중량%의 대추씨, 11~15중량%의 인삼, 9~12중량%의 복령, 21~34중량%의 지황, 3~5중량%의 죽엽, 3~5중량%의 복분자, 26~34중량%의 봉밀로 혼합하는 것을 특징으로 한다.At this time, the herbal mixture is 11 to 15% by weight jujube leaves, 3 to 5% by weight jujube seeds, 11 to 15% by weight ginseng, 9 to 12% by weight of Fuling, 21 to 34% by weight of turmeric, 3 to 5 It is characterized by mixing by weight of bamboo leaves, 3-5% by weight of bokbunja, 26-34% by weight of honey.
그리고 상기 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀이 혼합된 한방혼합물을 열수추출하여 제조된 열수추출액을 한방배지라 한다.And the hot water extract prepared by hot water extraction of the mixture of jujube leaves, jujube seeds, ginseng, fuyeong, jihwang, bamboo leaves, bokbunja, beeswax is called a herbal medium.
이때, 한방혼합물을 열수추출한 한방배지의 고형분 함량은 10~20중량%인 것 을 특징으로 한다.At this time, the solid content of the herbal medium from which the herbal mixture is extracted with hot water is characterized in that 10 to 20% by weight.
4. 혼합 및 멸균공정(제4공정)4. Mixing and sterilization process (4th process)
대추와 한방배지를 혼합한 후 멸균하는 공정으로,In the process of sterilization after mixing jujube and herbal media,
상기 제2공정에서 씨가 제거된 대추열매와 상기 제3공정에서 제조된 한방배지를 중량대비 3~5:1로 혼합한 후 100~150℃의 멸균기에서 20~50분간 멸균한다.The jujube fruit from which the seed was removed in the second step and the herbal medium prepared in the third step are mixed 3 to 5: 1 by weight, and then sterilized for 20 to 50 minutes in a sterilizer at 100 to 150 ° C.
대추열매와 한방배지를 혼합하는 것은 씨가 제거된 대추열매에 인삼, 복령, 지황, 죽엽, 복분자, 봉밀이 혼합되어 열수추출된 한방배지의 기능성 성분을 함유시키기 위함이다.The mixture of jujube fruit and herbal medium is to contain the functional components of the hot-water extracted herbal medium by mixing ginseng, bokyeong, jihwang, bamboo leaf, bokbunja and beeswax in the seed extract.
또한 대추열매와 한방배지를 혼합한 혼합물을 100~150℃의 멸균기에서 20~50분간 멸균하는 것은 대추열매와 한방배지에 잔존하는 미생물을 멸균함으로써 대추열매와 한방배지에 잔존하는 미생물에 의해, 유산균과 효모의 발효시 발효가 억제되는 것을 방지하고, 변질, 부패 등의 오염을 차단하기 위함이다.In addition, sterilizing the mixture of jujube fruit and herbal medium for 20 to 50 minutes in a sterilizer at 100-150 ° C. sterilizes the microorganisms remaining in jujube fruit and herbal medium by lactic acid bacteria by the microorganisms remaining in jujube fruit and herbal medium. This is to prevent fermentation from being suppressed during fermentation of yeast and to block contamination such as deterioration and rot.
5. 유산균 및 효모 배양공정(제5공정)5. Lactic acid bacteria and yeast culture process (5th process)
액체배지에 유산균 및 효모를 배양하는 공정으로,In the process of culturing lactic acid bacteria and yeast in liquid medium,
액체배지에 유산균과 효모를 혼합접종한 후 25~32℃, 140~180rpm의 진탕배양기를 이용하여 24~72시간 동안 배양한다.After mixed inoculation of lactic acid bacteria and yeast in a liquid medium, the culture medium is incubated for 24 to 72 hours using a shaker at 25 to 32 ° C and 140 to 180 rpm.
이때, 액체배지에 접종된 유산균과 효모는 중량대비 1:1로 혼합하는 것을 특징으로 한다.At this time, the lactic acid bacteria and yeast inoculated in the liquid medium is characterized in that 1: 1 by weight.
여기서, 상기 액체배지에 배양된 유산균은 락토바실러스 플란타룸(Lactobacillus plantarum), 락토바실러스 불가리커스(Lactobacillus bulgarcus), 락토바실러스 애시도필러스(Lactobacillus acidophilus) 중 어느 하나를 선택하여 사용하는 것을 특징으로 한다.Here, the lactic acid bacteria cultured in the liquid medium is characterized by using any one selected from Lactobacillus plantarum, Lactobacillus bulgarcus, Lactobacillus ashdophyllus (Lactobacillus acidophilus). do.
여기서, 상기 액체배지에 배양된 효모는 사카로마이세스 세레비시아(Saccharomyces cerevisiae), 사카로마이세스 엘립소이더스(Saccharomyces ellipsoideus), 사카로마이세스 바야누스(Saccharomyces Bayanus) 중 어느 하나를 선택하여 사용하는 것을 특징으로 한다.Here, the yeast cultured in the liquid medium is selected from Saccharomyces cerevisiae, Saccharomyces ellipsoideus, Saccharomyces Bayanus It is characterized by using.
한편, 상기 액체배지는 0.1~0.5중량%의 이스트 익스트랙트(yeast extract), 0.1~0.5중량%의 말트 익스트랙트(malt extract), 0.1~1중량%의 펩톤(peptone), 0.5~2중량%의 덱스트로스(dextrose), 96~99.2중량%의 정제수로 이루어진 것이고, pH를 4~6.5로 조정한 후 115~125℃에서 10~20분간 멸균하고 25~35℃로 냉각된 것이다.Meanwhile, the liquid medium is 0.1 to 0.5% by weight of yeast extract (yeast extract), 0.1 to 0.5% by weight of malt extract (malt extract), 0.1 to 1% by weight of peptone (peptone), 0.5 to 2% by weight Dextrose of (dextrose), consisting of 96 ~ 99.2% by weight of purified water, after adjusting the pH to 4 ~ 6.5 sterilized for 10-20 minutes at 115 ~ 125 ℃ and cooled to 25 ~ 35 ℃.
한편, 상기 액체배지에 혼합된 유산균과 효모를 진탕배양기에서 배양되는 혼합된 유산균과 효모의 균수는 1㎖당 1×106 ~ 1×108인 것을 특징으로 한다.On the other hand, the lactic acid bacteria and yeast mixed in the liquid medium is characterized in that the number of the mixed lactic acid bacteria and yeast cultured in a shake incubator is 1 × 10 6 ~ 1 × 10 8 per ml.
6. 접종 및 발효공정(제6공정)6. Inoculation and fermentation process (6th process)
대추와 한방배지가 혼합 및 멸균된 혼합물에 유산균과 효모가 배양된 배양액을 접종한 후 발효하는 공정으로,The process of inoculating fermentation after inoculating a culture medium in which lactic acid bacteria and yeast are cultivated in a mixture of jujube and herbal media mixed and sterilized.
상기 제4공정에서 대추와 한방배지가 혼합 및 멸균된 혼합물에 상기 제5공정에서 유산균과 효모가 배양된 배양액을 혼합하여 접종시킨 후 20~35℃의 발효기에서 24~72시간 동안 발효한다.The jujube and herbal medium is mixed and sterilized in the fourth step inoculated by inoculating the culture solution cultured with lactic acid bacteria and yeast in the fifth step and then fermented for 24 to 72 hours in a fermenter at 20 ~ 35 ℃.
대추와 한방배지가 혼합 및 멸균된 혼합물에 유산균과 효모가 배양된 배양액을 혼합하여 접종시킨 후 20~35℃의 발효기에서 24~72시간 동안 발효하는 것은 After inoculating a mixture of jujube and herbal media mixed and sterilized with a culture medium in which lactic acid bacteria and yeast were inoculated, fermentation for 24 to 72 hours in a fermenter at 20 to 35 ° C
대추와 한방배지가 혼합 및 멸균된 혼합물에 접종된 유산균과 효모에 의해 발효됨에 따라 유산균과 효모의 개체수가 증가되어 유산균과 효모가 가지고 있는 기능성을 활성화하기 위함이다.As jujube and herbal media are fermented by lactic acid bacteria and yeast inoculated into the mixed and sterilized mixture, the number of lactic acid bacteria and yeast is increased to activate the functionality of lactic acid bacteria and yeast.
이때, 대추와 한방배지가 혼합 및 멸균된 혼합물에 유산균과 효모가 배양된 배양액을 접종한 후 발효한 것을 기능성 한방대추라고 한다.At this time, the fermented after inoculating the culture medium cultured with lactic acid bacteria and yeast in the mixture and sterilized jujube and herbal medium is called functional herbal jujube.
7. 살균 및 건조공정(제7공정)7. Sterilization and drying process (7th process)
기능성 한방대추를 살균 및 건조하는 공정으로,In the process of sterilizing and drying functional herbal jujube,
상기 제6공정의 기능성 한방대추를 65~75℃의 건조기에서 90~150분간 건조하여 살균 및 건조한다.The functional herbal jujube of the sixth process is dried for 90 to 150 minutes in a dryer at 65 ~ 75 ℃ sterilization and drying.
기능성 한방대추를 건조기에서 살균 및 건조하는 것은 미생물의 생육조건을 저하시켜 기능성 한방대추에 있는 유산균과 효모의 개체수가 더욱 증식되는 것을 방지함과 동시에 증식된 유산균과 효모에 의한 물성의 변화를 방지하기 위함이다. Sterilization and drying of the functional herbal jujube in the dryer lowers the growth conditions of the microorganisms to prevent further growth of the lactic acid bacteria and yeast population in the functional herbal jujube and at the same time to prevent the change of physical properties by the proliferated lactic acid bacteria and yeast. For sake.
이하에서 실시예를 통하여 본 발명을 보다 구체적으로 설명한다. 그러나 하 기의 실시예는 본 발명을 구체적으로 예시하기 위한 것일 뿐, 본 발명의 권리범위를 제한하는 것이 아님은 당업자에게 있어서 명백한 사실이다. 즉, 본 발명의 단순한 변형 내지 변경은 당업자에 의하여 용이하게 실시될 수 있으며, 이러한 변형이나 변경은 모두 본 발명의 영역에 포함되는 것으로 볼 수 있다.Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are only intended to specifically illustrate the present invention, it is obvious to those skilled in the art that the scope of the present invention is not limited. That is, simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.
실시예 : 본 발명에 따라 제조된 기능성 발효대추의 추출액Example: Extract of Functional Fermented Jujube Prepared According to the Present Invention
흠집이나 상처가 나지않은 대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀을 선별한 후 아세트산과 증류수가 혼합된 혼합액(pH5~6)에 45초간 침지시키고, 흐르는 물에 세척한다.After screening jujube fruit, jujube leaf, jujube seed, ginseng, bokyeong, huangwang, bamboo leaf, bokbunja, and wheat not damaged, immersed in mixed solution (pH5 ~ 6) mixed with acetic acid and distilled water for 45 seconds, and running water Wash on.
선별한 후 세척된 대추열매, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀 중 대추열매는 열매의 씨 제거기를 이용하여 씨를 제거하며, 대추잎, 대추씨, 인삼, 복령, 지황, 죽엽, 복분자, 봉밀를 혼합한 후 증류수를 다시 혼합한 수 90℃에서 5시간 열수추출하여 한방배지는 제조한다.After screening, washed dates of jujube, jujube leaf, jujube seed, ginseng, bokyeong, jihwang, bamboo leaf, bokbunja, and beeswax, the jujube berry removes the seeds using the fruit seed remover, jujube leaf, jujube seed, ginseng, bokyeong, After mixing turmeric, bamboo leaf, bokbunja and beeswae, distilled water was mixed again and then hot water extracted at 90 ° C. for 5 hours to prepare a herbal medium.
씨가 제거된 대추에 제조된 한방배지를 혼합한 후 130℃의 멸균기에서 45분간 멸균한다.After mixing the herbal medium prepared in the seed was removed jujube sterilized for 45 minutes in a sterilizer at 130 ℃.
한편, 액체배지에 유산균과 효모를 혼합접종한 후 28℃, 160rpm의 진탕배양기를 이용하여 48시간 동안 배양한다.On the other hand, mixed inoculation of lactic acid bacteria and yeast in a liquid medium and incubated for 48 hours using a shaker at 28 ℃, 160rpm.
상기 대추와 한방배지가 혼합 및 멸균된 혼합물에 상기 유산균과 효모가 배양된 배양액을 혼합하여 접종시킨 후 30℃의 발효기에서 48시간 동안 발효한 후 70℃의 건조기에서 120분간 건조하여 건조, 살균한 후 120℃의 추출기를 이용하고 12 시간 열수추출하여 기능성 발효대추의 추출액을 제조한다.The jujube and herbal media were mixed and sterilized in a mixture in which the lactic acid bacteria and yeast cultures were mixed and inoculated, fermented in a fermenter at 30 ° C. for 48 hours, dried in a dryer at 70 ° C. for 120 minutes, dried and sterilized. After using 120 ℃ extractor and hot water extraction for 12 hours to prepare an extract of the functional fermented jujube.
비교예 : 일반적인 방법에 따라 제조된 대추의 추출액Comparative Example: Extract of Jujube Prepared According to a Common Method
흠집이나 상처가 나지않은 대추열매를 선별한 후 흐르는 물에 세척하고, 세척된 대추열매를 열매의 씨 제거기를 이용하여 씨를 제거하고, 120℃의 추출기를 이용하고 12시간 열수추출하여 대추의 추출액을 제조한다.After selecting the jujube fruit without scratches or scratches, wash it under running water, and remove the seed by using the seed remover of the fruit, and extract the jujube extract by using hot water extractor at 120 ℃ for 12 hours. Manufacture.
실험 1 : SOD(Superoxide dismutase) 유사활성 측정실험Experiment 1: Superoxide dismutase (SOD) similar activity measurement experiment
SOD Assay kit를 이용하여 슈퍼옥시드 아니온 라디칼(Superoxide anion radical, O2 -)의 소거 활성률(저해률)을 측정하는 것으로, 실시예와 비교예를 증류수에 20㎎/㎖로 희석한 후 450㎚의 흡광도에서 측정하였다.After diluting by measuring the scavenging rate (inhibition rate) of Examples and Comparative Examples in 20㎎ / ㎖ in distilled water - using SOD Assay kit super oxide anion radicals (Superoxide anion radical, O 2) The absorbance was measured at 450 nm.
(20 mg/ml)SOD-like activity
(20 mg / ml)
19.82
19.82
72.86
72.86
상기 표1를 통해 알 수 있는 바와 같이, 비교예의 추출액보다 실시예의 추출액이 SOD 유사활성이 전체적으로 높게 나타내고 있으며, 하기 도2를 통해서도 알 수 있다.As can be seen from Table 1, the extract of the Example shows a higher SOD-like activity as a whole than the extract of the comparative example, it can also be seen through Figure 2 below.
비교예의 추출액보다 실시예의 추출액이 SOD 유사활성이 전체적으로 높게 나타내고 있을 뿐만 아니라 70%이상으로 나타남에 따라, 노화의 주요 요인인 활성산소를 제거할 수 있는 항산화력이 탁월이 높은 것임을 알 수 있다.As compared with the extract of the comparative example, the extract of the example showed higher SOD-like activity as a whole as well as 70% or more, indicating that the antioxidant power capable of removing active oxygen, which is a major factor of aging, is excellent.
실험 2: 혈청지질에 대한 실험Experiment 2: Experiment with Serum Lipids
6주령의 실험용 흰쥐를 온도22℃, 습도50%에서 1주일간 적응시킨 후 일반사료를 급여하거나 고지혈증을 유발하기 위해 고지방식이(동물성지질 40%이상 함유된 식이)를 2주간 급여 후 4주의 실험기간 동안 실시예의 추출액과 비교예의 추출액을 경구투여한다.Six-week-old rats were acclimated for one week at a temperature of 22 ° C and a humidity of 50% for four weeks, followed by two weeks of high-fat diet (dietary lipid content of 40% or more) for two weeks to feed normal feed or cause hyperlipidemia. During the period, the extract of Example and the extract of Comparative Example are administered orally.
여기서, 5개의 실험군으로 나누어 실험을 하였다. 제1실험군(Normal control group, NC)은 일반사료를 급여한 실험군이고, 제2실험군(High fat diet control group, HC)은 고지방식이를 급여한 실험군이고, 제3실험군(Positive control group, PC)은 고지혈치료제(lovastatin) 투여한 실험군이고, 제4실험군(Pure fructus of Jujuba Hotwater extracts group, PZH)은 고지방식이를 급여한 실험군에 비교예를 투여한 실험군이고, 제5실험군(Fermented Jujuba Hotwater extracts group, FZH)은 고지방식이를 급여한 실험군에 실시예를 투여한 실험군이다.Here, the experiment was divided into five experimental groups. The first experimental group (Normal control group, NC) is the experimental group fed the normal feed, the second experimental group (High fat diet control group, HC) is the experimental group fed the high fat diet, the third experimental group (Positive control group, PC) ) Is the experimental group administered with lovastatin, and the fourth experimental group (Pure fructus of Jujuba Hotwater extracts group, PZH) was the experimental group administered the comparative example to the experimental group fed the high fat diet, and the fifth experimental group (Fermented Jujuba Hotwater). extracts group (FZH) is an experimental group in which an Example was administered to an experimental group fed high fat diet.
이때, 각각의 실험군은 사료와 물을 자유롭게 섭취하였다.At this time, each experimental group was free to feed and water.
그리고, 각각의 실험군에 4주간 급여한 후 12시간 절식한 후 에테르로 마취하고 개복하여 심장에서 채혈하고, 채취한 혈을 3시간 방치한 후 전체 콜레스테롤(Total cholesterol), 트리글리세라이드(중성지방, Triglyceride), 에이디엘 콜레스테롤(HDL-cholesterol), 엘디엘 콜레스테롤(LDL-cholesterol), 씨알에프(CRF )를 측정하였다. After feeding for 4 weeks for each experimental group, 12 hours of fasting, anesthetized with ether, opened, blood was collected from the heart, and the collected blood was left for 3 hours, then total cholesterol, triglyceride (triglyceride, Triglyceride). ), ADL cholesterol (HDL-cholesterol), LDL cholesterol (LDL-cholesterol), CRF (CRF) were measured.
(mg/dl)Total cholesterol
(mg / dl)
cholesterol
(mg/dl)HDL-
cholesterol
(mg / dl)
cholestero
(mg/dl)LDL-
cholestero
(mg / dl)
(mg/dl)CRF
(mg / dl)
상기 표2를 통해 알 수 있는 바와 같이, 고지방식이를 급여한 실험군인 HC의 중성지방 측정값보다 PC의 중성지방의 측정값, PZH의 중성지방의 측정값, FZH의 중성지방의 측정값이 더욱 낮게 나타내고 있다.As can be seen from Table 2, the measured value of the triglyceride of PC, the measured value of triglyceride of PZH, and the measured value of triglyceride of FZH are higher It is shown lower.
HC의 중성지방 측정값보다 PC의 중성지방의 측정값, PZH의 중성지방의 측정값, FZH의 중성지방의 측정값이 고지방식이를 급여한 실험군인 HC의 중성지방 측정값보다 낮은 것은 PC의 경우 고지혈치료제가 투여되었고, PZH의 경우 대추의 추출액이 투여되었고, FZH의 경우 유산균과 효모가 발효된 대추의 추출액이 투여되었기 때문이다.The measured values of PC triglycerides, PZH triglycerides and FZH triglycerides were lower than those of HC, which were fed high-fat diets. In this case, hyperlipidemia treatment was administered, extract of jujube was administered in case of PZH, and extract of jujube fermented with lactic acid bacteria and yeast was administered in case of FZH.
특히 PC보다 PZH와 FZG의 중성지방의 측정값이 더욱 낮게 나타남에 따라 고지혈치료제보다 대추나 유산균과 효모가 발효된 대추가 중성지방을 낮출 수 있게 하며, 이로 인해 혈관에 지방이 축적되는 것을 방지하여 동맥경화증과 같은 혈관계 질환에 효과가 있음을 알 수 있다.In particular, PZH and FZG triglycerides are lower than PCs, so jujube, lactic acid bacteria, and yeast fermented jujubes can lower triglycerides than antilipidemic drugs, thereby preventing fat from accumulating in blood vessels. It can be seen that it is effective in vascular diseases such as atherosclerosis.
실험 3 : 간지질에 대한 실험Experiment 3: Experiment on Liver Lipids
6주령의 실험용 흰쥐를 온도22℃, 습도50%에서 1주일간 적응시킨 후 일반사료를 급여하거나 고지혈증을 유발하기 위해 고지방식이(동물성지질 40%이상 함유된 식이)를 2주간 급여 후 4주의 실험기간 동안 실시예의 추출액과 비교예의 추출액을 경구투여한다.Six-week-old rats were acclimated for one week at a temperature of 22 ° C and a humidity of 50% for four weeks, followed by two weeks of high-fat diet (dietary lipid content of 40% or more) for two weeks to feed normal feed or cause hyperlipidemia. During the period, the extract of Example and the extract of Comparative Example are administered orally.
여기서, 5개의 실험군으로 나누어 실험을 하였다. 제1실험군(Normal control group, NC)은 일반사료를 급여한 실험군이고, 제2실험군(High fat diet control group, HC)은 고지방식이를 급여한 실험군이고, 제3실험군(Positive control group, PC)은 고지혈치료제(lovastatin) 투여한 실험군이고, 제4실험군(Pure fructus of Jujuba Hotwater extracts group, PZH)은 고지방식이를 급여한 실험군에 비교예를 투여한 실험군이고, 제5실험군(Fermented Jujuba Hotwater extracts group, FZH)은 고지방식이를 급여한 실험군에 실시예를 투여한 실험군이다.Here, the experiment was divided into five experimental groups. The first experimental group (Normal control group, NC) is the experimental group fed the normal feed, the second experimental group (High fat diet control group, HC) is the experimental group fed the high fat diet, the third experimental group (Positive control group, PC) ) Is the experimental group administered with lovastatin, and the fourth experimental group (Pure fructus of Jujuba Hotwater extracts group, PZH) was the experimental group administered the comparative example to the experimental group fed the high fat diet, and the fifth experimental group (Fermented Jujuba Hotwater). extracts group (FZH) is an experimental group in which an Example was administered to an experimental group fed high fat diet.
이때, 각각의 실험군은 사료와 물을 자유롭게 섭취하였다.At this time, each experimental group was free to feed and water.
그리고, 각각의 실험군에 4주간 급여한 후 12시간 절식한 후 에테르로 마취하고 개복하여 간에서 채혈하고, 채취한 혈을 3시간 방치한 후 전체 콜레스테롤(Total cholesterol), 트리글리세라이드(중성지방, Triglyceride)을 측정하였다. After 4 weeks of feeding to each experimental group, 12 hours of fasting, anesthesia with ether and laparotomy to collect blood from the liver, and left the collected blood for 3 hours, total cholesterol (triglyceride, triglyceride) ) Was measured.
(mg/g tissue)Total cholesterol
(mg / g tissue)
(mg/g tissue)Triglyceride
(mg / g tissue)
상기 표3을 통해 알 수 있는 바와 같이, 고지방식이를 급여한 실험군인 HC의 중성지방 측정값보다 PC의 중성지방의 측정값, PZH의 중성지방의 측정값, FZH의 중성지방의 측정값이 더욱 낮게 나타내고 있다.As can be seen from Table 3, the measured value of triglycerides of PC, the measured value of triglycerides of PZH, and the measured value of triglycerides of FZH are higher than the measured values of triglycerides of HC, the experimental group fed high fat diet. It is shown lower.
HC의 중성지방 측정값보다 PC의 중성지방의 측정값, PZH의 중성지방의 측정값, FZH의 중성지방의 측정값이 고지방식이를 급여한 실험군인 HC의 중성지방 측정값보다 낮은 것은 PC의 경우 고지혈치료제가 투여되었고, PZH의 경우 대추의 추출액이 투여되었고, FZH의 경우 유산균과 효모가 발효된 대추의 추출액이 투여되었기 때문이다.The measured values of PC triglycerides, PZH triglycerides and FZH triglycerides were lower than those of HC, which were fed high-fat diets. In this case, hyperlipidemia treatment was administered, extract of jujube was administered in case of PZH, and extract of jujube fermented with lactic acid bacteria and yeast was administered in case of FZH.
특히 PC보다 PZH와 FZG의 중성지방의 측정값이 더욱 낮게 나타남에 따라 고지혈치료제보다 대추나 유산균과 효모가 발효된 대추가 중성지방을 낮출 수 있게 하며, 이로 인해 간에 지방이 축적되는 것을 방지하여 지방간과 같은 간질환에 효과가 있음을 알 수 있다.In particular, PZH and FZG triglycerides are lower than PCs, so jujube, lactic acid bacteria, and yeast fermented jujubes can lower triglycerides than hyperlipidemic drugs, thereby preventing fatty liver from accumulating fatty liver. It can be seen that it is effective in liver diseases such as.
실험 4 : 혈당에 대한 실험Experiment 4: Experiment on Blood Sugar
6주령의 실험용 흰쥐를 온도22℃, 습도50%에서 1주일간 적응시킨 후 16시간 절식하고 STZ(Streptozotocin), 0.01M citrate buffer(pH4.5)에 50㎎/㎏/body weight 농도로 복강에 투여하여 당뇨를 유발시킨다.Six-week-old rats were acclimated for 16 hours at 22 ° C and 50% humidity, and then fasted for 16 hours and administered intraperitoneally at 50 mg / kg / body weight in STZ (Streptozotocin) and 0.01M citrate buffer (pH4.5). To cause diabetes.
이때, 24시간 후 꼬리정맥에서 채혈한 후 혈당농도가 300㎎/dl 이상일 경우 당뇨가 유발된 것으로 간주한다.At this time, after taking blood from the tail vein after 24 hours, if the blood glucose level is 300 mg / dl or more, diabetes is considered to be induced.
당뇨가 유발된 흰쥐에 일반 사료와 물을 자유롭게 섭취하게 하되, 혈당강하제인 acarbose와 실시예, 비교예를 1일 1회 경구투여한다.The diabetic rats were allowed to freely ingest the general feed and water, and the oral blood glucose lowering agent acarbose and Examples and Comparative Examples were administered once a day.
특히 실시예와 비교예를 경구투여할 경우 300mg/kg의 농도로 희석한다.In particular, when administered orally to Examples and Comparative Examples diluted to a concentration of 300mg / kg.
여기서, 5개의 실험군으로 나누어 실험을 하였다. 제1실험군(Normal control group, NC)은 당뇨유발하지 않고 일반사료를 급여한 실험군이고, 제2실험군(Diabetes control group, DC)은 당뇨유발하고 일반사료를 급여한 실험군이고, 제3실험군(Positive control group, PC)은 당뇨유발하고 일반사료를 급여 및 혈당가하제를 투여한 실험군이고, 제4실험군(Pure fructus of Jujuba Hotwater extracts group, PZH)은 당뇨유발하고 일반사료를 급여 및 비교예를 투여한 실험군이고, 제5실험군(Fermented Jujuba Hotwater extracts group, FZH)은 당뇨유발하고 일반사료를 급여 및 실시예를 투여한 실험군이다.Here, the experiment was divided into five experimental groups. The first experimental group (Normal control group, NC) is an experimental group that fed general feed without causing diabetes, and the second experimental group (Diabetes control group, DC) is an experimental group that induces diabetes and fed general feed, and the third experimental group (Positive) The control group (PC) is the experimental group that induces diabetes and the general diet is fed and hypoglycemic agent, and the fourth experimental group (Pure fructus of Jujuba Hotwater extracts group, PZH) is the diabetes-induced group Experimental group, the fifth experimental group (Fermented Jujuba Hotwater extracts group, FZH) is an experimental group that induced diabetes and fed general feed and Example.
그리고 각각의 실험군을 3일 간격으로 꼬리 정맥에서 채혈한 후 혈당측정기를 이용하여 혈당을 측정하였다.Then, each group of blood was collected from the tail vein at intervals of 3 days and then blood glucose was measured using a blood glucose meter.
5.71117.4 ±
5.71
0.96134.8 ±
0.96
2.81129.80 ±
2.81
±4.75123.80
± 4.75
4.75119.00 ±
4.75
11.41138.00 ±
11.41
7.06107.25 ±
7.06
29.04523.00 ±
29.04
72.96454.33 ±
72.96
35.67443.30 ±
35.67
43.46451.25 ±
43.46
51.13511.75 ±
51.13
4.92121.25 ±
4.92
68.73495.75 ±
68.73
13.70341.50 ±
13.70
18.80352.20 ±
18.80
50.57374.00 ±
50.57
47.94475.50 ±
47.94
3.63115.75 ±
3.63
31.65556.00 ±
31.65
18.81385.75 ±
18.81
39.46397.25 ±
39.46
2.91407.00 ±
2.91
35.86420.25 ±
35.86
상기 표4을 통해 알 수 있는 바와 같이, 당뇨유발 실험군인 DC의 혈당의 측정값보다 PC의 혈당의 측정값, PZH의 혈당의 측정값, FZH의 혈당의 측정값이 더욱 낮게 나타내고 있며, 하기 도3을 통해 알 수 있다.As can be seen from Table 4, the measured blood glucose level of PC, the measured blood glucose level of PZH, and the measured blood glucose level of FZH are lower than the measured blood glucose levels of DC, a diabetes-induced experimental group. This can be seen in 3.
DC의 혈당의 측정값보다 PC의 혈당의 측정값, PZH의 혈당의 측정값, FZH의 혈당의 측정값이 당뇨유발 실험군인 DC의 혈당의 측정값보다 낮은 것은 PC의 경우 혈당강하제가 투여되었고, PZH의 경우 대추의 추출액이 투여되었고, FZH의 경우 유산균과 효모가 발효된 대추의 추출액이 투여되었기 때문이다.The blood glucose lowering agent was administered to the PC, which was lower than the blood glucose measurement of the PC, the blood glucose of the PZH, and the blood glucose of the FZH. In the case of PZH, the extract of jujube was administered, and in the case of FZH, the extract of jujube fermented with lactic acid bacteria and yeast was administered.
특히 PC, PZH, FZG의 혈당의 측정값이 비슷하게 나타남에 따라, 대추나 유산균과 효모가 발효된 대추가 혈당강하제와 비슷한 효과가 있어, 당뇨병이 유발되는 것을 방지할 수 있다.In particular, as blood glucose values of PC, PZH, and FZG are similar, jujubes, lactic acid bacteria, and yeast fermented jujubes have similar effects to blood glucose lowering agents, thereby preventing diabetes.
도 1은 본 발명에 따른 기능성 발효 대추 제조방법을 개략적으로 도시한 공정흐름도이다. 1 is a process flow diagram schematically showing a functional fermented jujube manufacturing method according to the present invention.
도 2는 SOD(Superoxide dismutase) 유사활성 측정실험에서 각각의 실험군을 측정한 값을 나타내는 그래프이다.Figure 2 is a graph showing the values measured for each experimental group in the SOD (Superoxide dismutase) -like activity measurement experiment.
도 3은 혈당에 대한 실험에서 각각의 실험군을 측정한 값을 나타내는 그래프이다.Figure 3 is a graph showing the values measured for each experimental group in the experiment on blood glucose.
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020080050121A KR101012555B1 (en) | 2008-05-29 | 2008-05-29 | Method for preparing the functional fermented jujube and it's product |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020080050121A KR101012555B1 (en) | 2008-05-29 | 2008-05-29 | Method for preparing the functional fermented jujube and it's product |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20090124115A KR20090124115A (en) | 2009-12-03 |
KR101012555B1 true KR101012555B1 (en) | 2011-02-08 |
Family
ID=41686086
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020080050121A KR101012555B1 (en) | 2008-05-29 | 2008-05-29 | Method for preparing the functional fermented jujube and it's product |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR101012555B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104305189A (en) * | 2014-10-16 | 2015-01-28 | 山东天康生物科技股份有限公司 | Blood fat reducing and bowel relaxing liquid preparation and preparation method thereof |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101436394B1 (en) * | 2012-09-04 | 2014-09-04 | 김동환 | Method for preparing the fermented jujube and it's product |
CN112869134A (en) * | 2020-12-31 | 2021-06-01 | 厦门市天德善品生物科技有限公司 | Cordyceps sinensis, Guiyuan mushroom fungus quality, health food and preparation method thereof |
CN116034839A (en) * | 2023-02-13 | 2023-05-02 | 江西省科学院生物资源研究所 | Method for removing exocarp of glabrous greenbrier rhizome and cultivation method of glabrous greenbrier rhizome seedlings |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100478125B1 (en) | 2002-04-17 | 2005-03-22 | 완주군 | Process for Preparing Jujube Wine |
KR20090050539A (en) * | 2007-11-16 | 2009-05-20 | 김기호 | A making method of feed additive from jujube paste and jujube leaf fermentation |
-
2008
- 2008-05-29 KR KR1020080050121A patent/KR101012555B1/en not_active IP Right Cessation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100478125B1 (en) | 2002-04-17 | 2005-03-22 | 완주군 | Process for Preparing Jujube Wine |
KR20090050539A (en) * | 2007-11-16 | 2009-05-20 | 김기호 | A making method of feed additive from jujube paste and jujube leaf fermentation |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104305189A (en) * | 2014-10-16 | 2015-01-28 | 山东天康生物科技股份有限公司 | Blood fat reducing and bowel relaxing liquid preparation and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
KR20090124115A (en) | 2009-12-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104982928B (en) | A kind of japanese yew fruit health care ferment and preparation method thereof | |
CN108887674A (en) | A kind of preparation method of lycium ruthenicum composite enzyme liquid | |
CN107197966A (en) | A kind of method of microorganism ferment making GABA tea | |
CN106901015A (en) | Feed addictive containing active probiotic and its production and use | |
KR101314762B1 (en) | Manufacturing method of Fermented material extracted from ginseng seed and fermented food thereof | |
CN101407754A (en) | Production process of black fungus rough rice vinegar | |
KR101012555B1 (en) | Method for preparing the functional fermented jujube and it's product | |
KR100899220B1 (en) | Fermented Aloe and Manufacturing Method of Fermented Aloe and Functional food of Thereof Manufacturing | |
KR101984400B1 (en) | Process for producing antioxidant and anti-fatigue efficacy of mulberry vinegar | |
CN116731938B (en) | Composite microbial inoculum, application thereof in preparation of liver-protecting wine and liver-protecting wine | |
CN106987514B (en) | Liver-protecting fruit vinegar beverage rich in lactic acid bacteria and preparation method thereof | |
CN108977306A (en) | A kind of production method of health white wine | |
KR20160059137A (en) | MANUFACTURE OF FERMENTED Alliumhookeri FROM LACTIC ACID BACTERIA AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST | |
KR101448138B1 (en) | Beverage comprising extract of mulberry leaves and herbs | |
KR20060083190A (en) | Use of fermentation liquor using microorganisms from bamboo | |
CN114214152B (en) | Method for preparing roxburgh rose black glutinous rice wine through semi-solid fermentation | |
CN109673792A (en) | The preparation method of bitter buckwheat fermented tea | |
KR101318646B1 (en) | Vinegar including ginger and method thereof | |
KR101814941B1 (en) | Food composition for anti-oxidation comprising cockscomb flower extract short-term fermented by lactic acid bacteria and the method of preparation thereof | |
KR101620939B1 (en) | Method for preparing antiobese natural fermented vinegar using dendropanax morbiferum leveille and pear | |
CN106616977A (en) | Preparation method of edible cudrania tricuspidata ferment | |
CN114304609A (en) | Rose ferment with lipid-lowering effect and preparation method thereof | |
Dávila-Ortiz et al. | Pulque: a traditional mexican beverage with health benefits due to its nutritional composition | |
CN117257865B (en) | Preparation method and application of fermented traditional Chinese medicine composition for improving immunity of livestock | |
KR20200041119A (en) | Manufacturing method of seasoning comprising powdered larva of Protaetia brevitarsis and seasoning manufactured thereby |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
LAPS | Lapse due to unpaid annual fee |