KR100992717B1 - Organic-Inorganic Hybrid For Coagulation and Antimicrobial Applications And Manufacturing Method Thereof - Google Patents

Abstract

The present invention relates to a cohesive and antimicrobial organic-inorganic complex and a method for manufacturing the same, and more specifically, the coagulated and antimicrobial organic-inorganic complex is a horseradish (Moringa oleifera Lamarck) seeds or horseradish (Moringa oleifera Lamarck) C) a cationic peptide containing two or more amino acids of the same or different types of amino acids of glutamic acid, proline, methionine or arginine extracted from the residual press cake extracted from the oil of the seed; and ion exchanged with a metal cation of a layered silicate mineral; It relates to a cohesive and antimicrobial organic-inorganic complex and a method for producing the same characterized in that the cationic peptide 300 ~ 600 mg is contained in 1g of the layered silicate mineral.

In the present invention described above, the precipitate aggregated into the organic-inorganic composite is large and aggregation occurs quickly, thereby increasing the aggregation efficiency. In addition, since the dissolved heavy metal in the flocculated precipitate and the substance causing turbidity are not released, the precipitate does not cause colloidal contamination caused by re-dissolution in the treated water, and the strain is not re-diffused so that recontamination is not caused.

Aggregation, Antibacterial, Cationic, Peptides, Layered Silicate Minerals, Inorganic Inorganic Complex, Capture

Description

Organic-Inorganic Hybrid For Coagulation and Antimicrobial Applications And Manufacturing Method Thereof}

The present invention relates to an organic-inorganic complex for agglomeration and antibacterial and a method for preparing the same, and more specifically, the agglomeration and antimicrobial organic-inorganic complex for agglomeration, antibacterial function and ion of layered silicate mineral of horseradish extract cationic peptide. It relates to agglomeration and antimicrobial organic-inorganic complex formed by combining exchange and adsorption functions, and a method of manufacturing the same.

      It is mainly used to sediment negatively charged colloidal particles that cause turbidity such as mud particles, organic matter, bacteria, algae, pigments, colloids, and chemical contaminants in contaminated surface water and various wastewaters. (Poly Aluminum Chloride, PAC), Poly Aluminum Chloride Silicate (PACS), Poly Aluminum Silicate Sulfate (PASS), and Polymer Coagulant, an organic polymer, are used. As a coagulant to increase the acid, alkali, activated silica, polyelectrolytes, clay, bentonite, etc. are added to maximize the coagulation activity.

      In addition, as an alternative eco-friendly material, attempts to use the coagulant by extracting the coagulant active peptide from natural plants such as legumes, corn, and rice have been actively progressed.

      However, Alum has a problem that can cause Alzheimer's disease (dementia), and when using the metallic or polymer flocculant, an artificial chemical flocculant remains in the treated water.

      In addition, since the metallic or polymer flocculant is included in the sludge after treatment, problems such as secondary environmental pollution are raised during sludge recycling, and at the same time, problems such as contamination of the environment when sludge incineration occurs.

In order to solve this problem, a technique related to a water treatment flocculant manufacturing process (US Patent No. 6,890,565) consisting of horseradish seed protein serving as a primary flocculant for treating contaminated water is disclosed. However, the flocculant manufacturing process for water treatment is preferably used within the shortest time after extraction because the flocculant active material extracted by adding sodium chloride is organic, and there are problems such as color change and reduced activity during transportation and storage. In addition, the sediment is redissolved again in the treated water over time, causing problems of colloidal contamination and the problem of recontamination by the re-spreading of the removed strain.

Therefore, the object of the present invention is to cause dementia and environmental pollution and extract the aggregated effective substance for a long time, the precipitate is re-dissolved in the treated water, causing colloidal contamination, recontamination due to the re-spreading of the strain Agglomeration, antimicrobial and ion exchange functions of layered silicate minerals of edible horseradish extract cationic peptides, which is an edible plant, have been formed to form an organic-inorganic complex and an organic-inorganic complex for antibacterial and antibacterial treatment The purpose is to provide a method.

      In order to solve the object as described above, the present invention is an organic-inorganic complex for aggregation and antibacterial,

Extract two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from the residual press cake extracted from the oil of the Moringa oleifera Lamarck seed or the Moringa oleifera Lamarck seed. Ion-exchanging the cationic peptide containing; and the metal cation of the layered silicate mineral; wherein 10 to 700 mg of the cationic peptide is trapped and contained in 1 g of the layered silicate mineral, preferably the ions in 1 g of the layered silicate mineral. It provides an aggregated and antimicrobial organic-inorganic complex characterized in that the sex peptide 300 ~ 600mg is contained.

     The layered silicate mineral may be a modified layered silicate mineral wherein the hydrophilic silicate mineral is modified with an organic cationic surfactant, and preferably, the modified layered silicate mineral may be modified with an aqueous solution of Hexadecyltrimethylammonium (HDTMA). .

      In this case, the modified layered silicate mineral is mixed with 200 ml of an aqueous solution of organic HDTMA in 5 g of the layered silicate mineral, stirred with a shaker for 24 hours, and then separated for 2 hours at a speed of 10,000 rpm to remove the supernatant. It may be a rough modified layered silicate mineral.

      In addition, the present invention is an organic-inorganic complex for aggregation and antibacterial,

Extract two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from the residual press cake extracted from the oil of the Moringa oleifera Lamarck seed or the Moringa oleifera Lamarck seed. The cationic peptide containing; is adsorbed to the layered silicate mineral from which the metal cation has been removed; 10 to 700 mg of the cationic peptide is trapped and contained in 1 g of the layered silicate mineral, preferably in the 1 g of the layered silicate mineral. It provides an organic-inorganic complex for aggregation and antibacterial, characterized in that the ionic peptide 300 ~ 600mg is contained.

     The layered silicate mineral may be a modified layered silicate mineral wherein the hydrophilic silicate mineral is modified with an organic cationic surfactant, preferably, the modified layered silicate mineral may be modified with an aqueous solution of Hexadecyltrimethylammonium (HDTMA). have.

      In this case, the modified layered silicate mineral is mixed with 200 ml of an aqueous solution of organic HDTMA in 5 g of the layered silicate mineral, stirred with a shaker for 24 hours, and then separated for 2 hours at a speed of 10,000 rpm to remove the supernatant. It may be a rough modified layered silicate mineral.

      In addition, the present invention is an organic-inorganic complex for aggregation and antibacterial,

Extract two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from the residual press cake extracted from the oil of the Moringa oleifera Lamarck seed or the Moringa oleifera Lamarck seed. The cationic peptide containing; is adsorbed to a porous material capable of adsorbing the cationic peptide; 10g to 700mg of the cationic peptide is contained in 1g of the porous material, preferably in 1g of the porous material It provides an organic-inorganic complex for aggregation and antibacterial, characterized in that the cationic peptide 300 ~ 600mg is contained.

The porous material may be a porous material modified with an organic cationic surfactant. Preferably, the porous material may be a modified porous material in which the porous material is modified with an aqueous solution of organic hexadecyltrimethylammonium (HDTMA).

     At this time, in the modified porous material, and mixed with 200ml of the organic HDTMA aqueous solution to 5g of porous material and stirred for 24 hours with a shaker (shaker), the process of removing the supernatant by separating for 2 hours at a speed of 10,000rpm It may be a rough modified porous material.

      In addition, the present invention is a cationic peptide extraction method,

     Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder;

      Immersing the seed powder in n-hexane and stirring to remove oil;

      Separating the solid and the supernatant by filtration with filter paper after removing the oil;

     Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities;

Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) in the remaining filtrate with heating at 80 to 100 ° C. until a pH of 7.5 to 8.5 is formed to form an aggregated active aqueous solution;

      Concentrating the coagulated active aqueous solution under reduced pressure with a rotary vacuum evaporator and freeze drying to form a dry powder; And

      It provides a cationic peptide extraction method comprising the step of forming a cationic peptide powder by separating the sodium ions by electrophoresis after the aqueous solution by adding distilled water to the dry powder.

      In addition, the other method of extracting the cationic peptide of the present invention,

      Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder, extracting oil, and then making a seed cake powder;

      Dipping the seed cake powder in ether and then stirring to remove oil;

      Separating the solid and the supernatant by filtration with filter paper after removing the oil;

      Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities;

Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) in the remaining filtrate with heating at 80 to 100 ° C. until a pH of 7.5 to 8.5 is formed to form an aggregated active aqueous solution;

      Concentrating the coagulated active aqueous solution under reduced pressure with a rotary vacuum evaporator and freeze drying to form a dry powder; And

      It provides a cationic peptide extraction method comprising the step of forming a cationic peptide powder by separating the sodium ions by electrophoresis after the aqueous solution by adding distilled water to the dry powder.

       And, as the cationic peptide extraction method of the present invention,

      Powder manufacturing step of making a seed powder by peeling and pulverizing the horseradish (Moringa oleifera Lamark) plant seeds;

      An oil removal step of removing oil contained in the seed powder;

An eluting step of eluting the extracted peptide in an aqueous solution of sodium bicarbonate (NaHCO ₃ ) by extracting the peptide from the powder obtained through the oil removal step;

      A step of obtaining an agglomerated active solution to remove the impurities of the aqueous solution which has passed through the elution step to obtain an agglomerated active solution;

      A peptide concentration and separation step of concentrating and separating only peptides from the aggregation active aqueous solution which has undergone the step of obtaining the aggregation active aqueous solution;

      Purifying step of purifying only pure cationic peptide by removing the anionic substances remaining as impurities by passing the obtained product passed through the peptide concentration and separation step through a cation exchange resin column:

      It provides a cationic peptide extraction method comprising a; powder forming step of forming a powder through the obtained product after the purification step.

      Preferably, the oil removal step, the seed powder by immersing the seed powder in the oil removal solvent by a compression method or a ratio of 1: 2 to weight, and stirred for about 20 to 30 minutes at a rate of 200rpm or less and then the oil content In order to completely remove the solvent, put it in a drying pan, put it in an oven, and leave it at a temperature of 40 degrees Celsius for about 24 to 48 hours to evaporate the organic solvent; repeat the process of removing oil by repeating the process 3-5 times. It may be an oil removal step.

      On the other hand, the oil removal solvent may be alcohol or n-hexane.

The elution step, the powder obtained through the oil removal step is put into an aqueous solution of sodium bicarbonate (NaHCO ₃ ) having a concentration of 1M per 100g powder 1L aqueous solution adjusted to pH 7.5 ~ 8.5 at a temperature of 15 ~ 100 ℃, preferably Preferably, the step of eluting the peptide into the aqueous solution through stirring while heating at a temperature of 50 ~ 60 ℃.

      In addition, the step of obtaining the coagulant active aqueous solution may be a step of obtaining a coagulant active aqueous solution including a process of removing solid impurities or other impurities through an integral filtering device.

    In addition, the step of concentrating and separating the peptide may be a step of concentrating and separating the peptide through a cellulose membrane of 14 kDa or less or an ultrafiltration process of the coagulant active aqueous solution obtained through the step of obtaining the coagulant active aqueous solution.

      The purification step is carried out through a cationic exchange resin tower for 36 to 48 hours at a rate of 5 ml / min in order to capture only pure cationic peptides from the obtained product after the peptide concentration and separation step. It may be a step of removing and purifying.

      In addition, the powder forming step may be a powder forming step of forming a seed powder by freeze-drying the peptide-containing solution, which has undergone the concentration and separation step, under reduced pressure at a temperature of minus 80 degrees for 24 to 48 hours.

      In addition, the present invention may include a cationic peptide powder prepared by the cationic peptide extraction method.

      In addition, the aggregation and antimicrobial organic-inorganic composite manufacturing method of the present invention,

     Dispersing the cationic peptide powder extracted by the cationic peptide extraction method in deionized water;

     Dipping the layered silicate mineral in deionized water to remove metal cations bound in the lattice structure using ultrasonic waves;

     Forming a peptide-mineral adsorbate by stirring and adsorbing the cationic peptide powder dispersed in the deionized water and the layered silicate mineral from which the metal cation has been removed for 18 to 48 hours; And

     Filtering the peptide-mineral adsorbate and removing moisture, followed by drying in an oven at 70 to 100 ° C. for 18 to 36 hours or freeze drying under reduced pressure to form an organic-inorganic complex; Provide a method.

The present invention also provides a flocculant or an antimicrobial agent containing the aggregation and antibacterial organic-inorganic complex as an active ingredient.

      The present invention also provides a filter or adsorbent containing the aggregated and antimicrobial organic-inorganic complex as an active ingredient.

      The present invention also provides a deodorant containing the aggregation and antimicrobial organic-inorganic complex as an active ingredient.

      The present invention also provides a food additive or feed additive containing the aggregation and antimicrobial organic-inorganic complex as an active ingredient.

      In addition, the present invention may be a cosmetic additive or toothpaste additive containing the aggregation and antimicrobial organic-inorganic complex as an active ingredient, it may provide a cosmetic or toothpaste.

On the other hand, the present invention is the deposition of any fiber, food additives, feed additives, plastic additives, cosmetic additives, pharmaceutical additives, health supplements additives using the cationic peptide powder or the organic and inorganic complex for the aggregation and antibacterial as an active ingredient Can provide.

      In the aggregation and antibacterial organic-inorganic complex of the present invention and a method for producing the same, the cationic peptide is separated and acts as a flocculant and an antimicrobial agent when the aggregation and antimicrobial organic-inorganic complex is hydrated, and the layered silicate mineral is dissolved heavy metal. At the same time, the flocculation flocs (agglomerated precipitate, flocculated suspended solids) formed by being present in the colloidal phase while adsorbed are large and agglomerated quickly, thereby increasing the flocculation efficiency.

      In addition, the cationic peptide is not separated again after being precipitated into the flocculation flocs and the dissolved heavy metal in the flocculation flocs and the substance causing turbidity are not released, so that the precipitate does not cause colloidal contamination caused by re-dissolution in the treated water. And does not cause recontamination since the strain is not respread.

In addition, since the aggregation and antimicrobial organic-inorganic complex is formed by the cationic peptide and the layered silicate mineral is environmentally friendly, recycling of the sludge formed during water treatment, ocean dumping and incineration is advantageous.

      The aggregated and antimicrobial organic-inorganic complex of the present invention is glutamic acid, proline, methionine or arginine extracted from residual press cake after extracting oil of horseradish (Moringa oleifera Lamarck) seeds or horseradish (Moringa oleifera Lamarck) seeds. A cationic peptide containing two or more amino acids of the same kind or different kinds of amino acids of; ion-exchanged with a metal cation of a layered silicate mineral; wherein 10 to 700 mg of the cationic peptide is trapped in 1 g of the layered silicate mineral; Preferably, the ionic peptide 300 to 600 mg is trapped and included in 1 g of the layered silicate mineral. The layered silicate mineral may be a modified layered silicate mineral wherein the hydrophilic silicate mineral is modified with an organic cationic surfactant, and preferably, the modified layered silicate mineral may be modified with an aqueous solution of Hexadecyltrimethylammonium (HDTMA). .

     In the modified layered silicate mineral, 5 g of the layered silicate mineral was mixed with 200 ml of an organic HDTMA aqueous solution, stirred for 24 hours with a shaker, and then separated for 2 hours at a speed of 10,000 rpm to remove the supernatant. It may be a modified layered silicate mineral.

    Alternatively, two or more of the same or different types of amino acids of glutamic acid, proline, methionine or arginine extracted from the residual press cake extracted from oil of the Moringa oleifera Lamarck seed or the Moringa oleifera Lamarck seed. A cationic peptide containing amino acid; adsorbed onto a layered silicate mineral from which metal cations have been removed; and 10 to 700 mg of the cationic peptide is contained in 1 g of the layered silicate mineral, preferably 1 g of the layered silicate mineral 300 to 600 mg of the ionic peptide contained in the trap is included. The layered silicate mineral may be a modified layered silicate mineral in which the hydrophilic silicate mineral is modified with an organic cationic surfactant, and preferably, may be a modified layered silicate mineral in which the hydrophilic layered silicate mineral is modified with an aqueous solution of Hexadecyltrimethylammonium (HDTMA). .

     In the modified layered silicate mineral, 5 g of the layered silicate mineral was mixed with 200 ml of an organic HDTMA aqueous solution, stirred for 24 hours with a shaker, and then separated for 2 hours at a speed of 10,000 rpm to remove the supernatant. It may be a modified layered silicate mineral.

      At this time, the cationic peptide is ion exchanged or adsorbed with a cation of the layered silicate mineral, and the agglomerated and antimicrobial organic-inorganic complex formed by trapping the cationic peptide in the layered silicate mineral is called a layered agglomerated and antimicrobial organic-inorganic complex. All.

      Here, the aggregation and antimicrobial organic-inorganic complex is formed by combining the antimicrobial activity of the horseradish extract cationic peptide, which is an edible plant, the adsorption function of the layered silicate mineral and the ion exchange function that can desorb the metal cation.

      At this time, the aggregation and antimicrobial organic-inorganic complex is easier to store and transport than the edible plant extract only by ion exchange and adsorption of the cationic peptide to the layered silicate mineral, there is no change over time.

      In addition, the aggregation and antimicrobial organic-inorganic complex is the cationic peptide is ion exchanged with the metal cation of the layered silicate mineral or adsorbed to the layered silicate mineral.

      When the aggregated and antimicrobial organic-inorganic complex is hydrated, the cationic peptide separates and functions as a flocculant and an antimicrobial agent, and since the layered silicate mineral adsorbs the dissolved heavy metal and exists in colloidal form, the layered silicate mineral and the dissolved heavy metal The flocculation floes formed (agglomerated precipitate, flocculated flocculent) are large and agglomeration is quick, so that the flocculation efficiency is increased.

      In addition, the aggregated and antimicrobial organic-inorganic complex is not separated again after the cationic peptide precipitates into the flocculation floes, so that the substance causing turbidity with the dissolved heavy metal in the flocculation flocs is not released.

     Therefore, the aggregated and antimicrobial organic-inorganic complex does not cause colloidal contamination caused by the re-dissolution of the precipitate in the treated water, and does not cause recontamination since the strain is not re-spread.

     In addition, since the aggregation and antimicrobial organic-inorganic complex is formed by the cationic peptide and the layered silicate mineral is environmentally friendly, recycling of the sludge formed during water treatment, ocean dumping and incineration is advantageous.

     In addition, agglomeration and antimicrobial organic-inorganic composites exhibit the properties of materials with functions such as antibacterial deodorization, far-infrared emission, and anion emission, so as to be applied to various applications as fiber deposition, food additives, feed additives, plastic additives, cosmetic additives, etc. Can be used in products.

In this case, the sheet silicate mineral is bangbiseok _{(NaAlSi 2 O 6 · H 2} O), eoanseok _{(KCa 4 FSi 8 O 2 0} · 8H2O), chabazite _{(mCa 7 Si 26 Al 14 O} 88 · 40H 2 O + n _{(Na, K) 4Ca 3 Si} 3 0Al 10 O 80 · 4H 2 O), sodium zeolite _{(Na 2 Al 2 Si 3 O} 10 · 2H 2 O), hyul randayi agent _{(CaAl 2 Si 7 O 18 ·} 6H 2 O ), SteelBite (CaAl ₂ Si ₇ O ₁₈ · 7H ₂ O), Lomonite (CaAl ₂ Si ₄ O ₁₂ · 4H ₂ O), Inesite (H ₂ (Mn, Ca) ₆ Si ₆ O ₁₉ · 3H ₂ Zeolites such as O), montmorillonite ((Na, Ca) _0.33 (Al, Mg) ₂ (Si ₄ O ₁₀ ) (OH) ₂ nH ₂ O), kaolinite (Al ₂ Si ₂ O ₅ (OH) ₄ ), Dolomite (K (OHF ₂ ) ₂ Al ₃ Si ₃ O ₁₀ ), Biotite (K (Mg, Fe) ₃ AlSi ₃ O ₁₀ (OH) ₂ ), Gold mica (KMg ₃ (AlSi ₃ ) O ₁₀ (F, OH ) ₂ ) and the like.

Here, the metal cation is Na ⁺ , K ⁺ , Mg ^{2 +} , A Ca ^{+ 2} or Al ^{+ 3} ion.

Since the component of the layered silicate mineral is as described above, the metal cation that is ion-exchangeable with the cationic peptide is Na ⁺ , K ⁺ , Mg ^{2 +} , The like Ca ^{+ 2} or Al ^{+ 3} ion.

    In addition, the cationic peptide is a cationic peptide containing two or more amino acids of the same or different kinds of amino acids of glutamic acid, proline, methionine, or arginine, and is a cationic peptide capable of ion exchange with the metal cation of the layered silicate mineral. .

       Here, the cationic peptide extraction method,

      Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder;

      Immersing the seed powder in n-hexane and stirring to remove oil;

      Separating the solid and the supernatant by filtration with filter paper after removing the oil;

     Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities;

Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) to 80 to 100 ° C. until the pH is 7.5 to 8.5 in the remaining filtrate to form an aggregated active aqueous solution;

Pressure-concentrating the flocculent active aqueous solution with a rotary vacuum evaporator and then freeze drying to form a dry powder; And

And distilled water is added to the dry powder to form an aqueous solution, followed by separation of sodium ions by electrophoresis to form a cationic peptide powder.

Here, the cationic peptide extraction method is a method of extracting from the horseradish (Moringa oleifera Lamarck) plant seeds.

      In addition, another extraction method of the cationic peptide,

      Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder, extracting oil, and then making a seed cake powder;

      Dipping the seed cake powder in ether and then stirring to remove oil;

      Separating the solid and the supernatant by filtration with filter paper after removing the oil;

     Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities;

Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) to 80 to 100 ° C. until the pH is 7.5 to 8.5 in the remaining filtrate to form an aggregated active aqueous solution;

      Concentrating the coagulated active aqueous solution under reduced pressure with a rotary vacuum evaporator and freeze drying to form a dry powder; And

      And distilled water is added to the dry powder to form an aqueous solution, followed by separation of sodium ions by electrophoresis to form a cationic peptide powder.

      Here, another method of extracting the cationic peptide is a method of extracting oil from the remaining horseradish (Moringa oleifera Lamarck) plant seed cake powder.

      In addition, the aggregation and antimicrobial organic-inorganic composite manufacturing method of the present invention is

      Dispersing the cationic peptide powder extracted by the cationic peptide extraction method in deionized water;

      Dipping the layered silicate mineral in deionized water to remove metal cations bound in the lattice structure using ultrasonic waves;

      Forming a peptide-mineral adsorbate by stirring and adsorbing the cationic peptide powder dispersed in the deionized water and the layered silicate mineral from which the metal cation has been removed for 18 to 48 hours; And

      And filtering the peptide-mineral adsorbate and removing moisture, followed by drying in an oven at 70 to 100 ° C. for 18 to 36 hours or freeze drying under reduced pressure to form an organic-inorganic complex.

      Here, the organic-inorganic complex production method is formed by combining and adsorbing the cationic peptide powder extracted by the cationic peptide extraction method with the layered silicate mineral from which metal cations in the lattice are removed.

     In addition, the present invention,

    Extract two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from the residual press cake extracted from the oil of the Moringa oleifera Lamarck seed or the Moringa oleifera Lamarck seed. The cationic peptide containing; is adsorbed to a porous material capable of adsorbing the cationic peptide; 10 ~ 700mg of the cationic peptide is trapped in 1g of the porous material, preferably the ionic in 1g of the layered silicate mineral Peptide 300 ~ 600mg is characterized in that it is contained. The porous material may be a modified porous material modified with an organic cationic surfactant. Preferably, the porous material may be a modified porous material modified with an aqueous solution of organic hexadecyltrimethylammonium (HDTMA).

     At this time, in the modified porous material, 5g of porous material was mixed with 200ml of organic HDTMA aqueous solution and stirred for 24 hours with a shaker (shaker), and then separated for 2 hours at a speed of 10,000rpm to remove the supernatant It may be a modified porous material.

     Here, the porous material is charcoal, activated carbon, glass beads, kaolin balls, volcanic clavicle rock, silica, alumina or titania.

    At this time, the cationic peptide is adsorbed to the porous material, the aggregation and antimicrobial organic-inorganic complex formed by trapping the cationic peptide in the porous material is called porous aggregation and antimicrobial organic-inorganic complex.

     The porous aggregate and antimicrobial organic-inorganic complex for adsorbing the cationic peptide and the porous material has the same function as the layered aggregation and antimicrobial organic-inorganic complex for ion exchange and adsorbate of the cationic peptide and the layered silicate mineral. .

     Therefore, since it does not change the thermal or chemical environment affecting the properties of each material in the manufacturing process, various properties such as adsorption, deodorization, and antibacterial of the individual material can be exhibited as it is, so that the organic-inorganic composite for porous aggregation and antibacterial It is possible to add cohesive and antimicrobial properties of the cationic peptide to adsorption, filtration, antibacterial, deodorization, mineral nutrients, far infrared rays, anions, etc., which are used for porous materials, so that existing food or feed additives, fiber treatment agents, plastic additives and cosmetics can be added. It can be used for various purposes such as additives and deodorants.

      The porous organic-inorganic composite manufacturing method is to use a porous material instead of the layered silicate mineral in the method of preparing a layered organic-inorganic composite formed by adsorption.

      Figure 1 shows the manufacturing process of the organic-inorganic complex according to the present invention, as shown in Figure 1, after removing the metallic cations from the layered silicate mineral and plant exchange cation peptide extracted from horseradish (Moringa oleifera Lamarck) and ion exchange and adsorption To form an organic-inorganic complex or by adsorbing a plant extract cationic peptide extracted from horseradish (Moringa oleifera Lamarck) to a porous material to form an organic-inorganic complex.

      The present invention also provides a flocculant or an antimicrobial agent containing the aggregation and antibacterial organic-inorganic complex as an active ingredient.

      The present invention also provides a filter or adsorbent containing the aggregated and antimicrobial organic-inorganic complex as an active ingredient.

      The present invention also provides a deodorant containing the aggregation and antimicrobial organic-inorganic complex as an active ingredient.

      The present invention also provides a food additive or feed additive containing the aggregation and antimicrobial organic-inorganic complex as an active ingredient.

      Hereinafter, the present invention will be described in detail by way of examples.

      However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.

       Example 1 layered flocculation and antimicrobial organic-inorganic composite manufacturing process

       1-1 Agglomeration and Antimicrobial Extraction Process from Horseradish Tree Seeds

In order to extract cationic peptides from the seeds of Moringa oleifera Lamarck, an edible plant, 100 g of powder obtained by separating fresh seeds from the bark was first immersed in 200 ml n-hexane (M-Hexane), followed by magnetic stirrer. After stirring for 30 minutes to remove the oil, and filtered by Whatman No. 3 filter, the solids and the supernatant is separated, 100ml of distilled water is added to the solids and stirred with a stirrer for about 30 minutes and then again Watts Filter by Whatman 3 times to remove impurities, and heat the sodium bicarbonate (NaHCO ₃ , purity 99.9%) powder to the remaining filtrate at 90 ℃ until it reaches pH 8. 120 ml of agglomerated active solution was prepared by stirring for 30 minutes, and concentrated under reduced pressure with a rotary vacuum evaporator, followed by freeze drying to obtain 6.5 g of a powder. .

      Then, distilled water was added to the powder to form an aqueous solution, and then sodium ions were separated by electrophoresis to extract pure cationic peptides.

      Agglomeration and Antimicrobial Extraction Process from 1-2 Horseradish Seed Cakes

Extract the oil in the same way as the powder made from fresh seed of Moringa oleifera Lamarck from the shell, and dilute 100 g of the remaining seed cake powder in 200 ml ether to stir and remove the oil. After filtering by Whatman 3 times, the solids and the supernatant were separated, 100 ml of distilled water was added to the solids, stirred with a stirrer for about 30 minutes, and filtered by Whatman 3 again to remove impurities. Sodium bicarbonate (NaHCO ₃ , purity 99.9%) powder was added to the remaining filtrate at a temperature of 90 to 100 ° C., and stirred for about 30 minutes using a magnetic stirrer. After manufacturing, the mixture was concentrated under reduced pressure with a rotary vacuum evaporator and freeze-dried to obtain 6 g of powder.

     Distilled water was added to the powder to form an aqueous solution, and sodium ions were separated by electrophoresis to extract pure cationic peptides.

      1-2- (1) Agglomeration and Antimicrobial Extraction Process from Horseradish Seeds.

     The fresh seeds of horseradish (Moringa oleifera Lamarck) were separated from the shells and pulverized, and the powder obtained was immersed in n-hexane at a ratio of 1: 2 to weight, stirred at 100 rpm for 30 minutes to remove oil. After repeating the process of the compressor three times to remove the oil as much as possible, and put it in a drying pan to remove the n-hexane, and put it in an oven and dried for 48 hours at 30 ℃ to completely evaporate the n-hexane after 1 L aqueous solution adjusted to pH 8 was added to an aqueous solution of sodium chloride (NaCl) having a concentration of 1 M per 100 g of powder, and the peptide was eluted by stirring while heating at a temperature of 50 ° C., and then filtered by Whatman No. 3 Then, the solids were separated to obtain an aggregated active aqueous solution, and the resulting aggregated active aqueous solution was concentrated and separated only through the 10 kDa size cellulose membrane and concentrated to separate the cationic peptide. The cationic peptide was extracted by freeze drying under reduced pressure at 80 degrees for 48 hours.

      Montmorillonite Deion Process for 1-3 Layered Silicate Minerals

      50 g of montmorillonite (hereinafter referred to as Na-Mont) in a layered silicate mineral having a particle diameter of about 5 μm was prepared, immersed in 500 ml of deionized water, and then repeatedly generated ultrasonic waves five times for 30 minutes each. Metal cations bound in the stomach were removed.

       1-4 Aggregation and Antimicrobial Cationic Polypeptides with Na-Mont

      The organic-inorganic complex that modified the hydrophilic layered silicate mineral with an organic cationic surfactant is described in "Competitive Desorption of Phenolic Compounds from HDTMA-Bentonite, Wonsik, Korea Society of Soil and Groundwater Environment, 2001 General Conference and Spring Conference" It is widely used as a sorbent to maximize the adsorption amount. The Na-Montmorillonite used in this experiment has an average particle diameter of 15 µm in the manufacturer's supply data, and shows a cation exchange capacity of 105 meq / 100 g. The mixture was mixed with an organic HDTMA aqueous solution and reacted using a shaker for 24 hours, and separated for 2 hours at a speed of 10,000 rpm to remove the supernatant to complete the modified organic montmorillonite. 50 g of each cationic peptide obtained in Example 1-2- (1) was dispersed in 250 ml of deionized water, and then stirred evenly with a shaker for 24 hours to complete adsorption. After removing water, the mixture was put in an oven at about 80 ° C. and dried for 24 hours to complete the organic-inorganic complex in which the vegetable cationic protein and Na-Mont were combined.

      1-5 Adsorption amount calculation

      As a result of the isothermal adsorption experiment on Na-Mont, adsorption equilibrium was found to adsorb about 13.6 mg per gram of Na-Mont. The B-point at which the adsorption equilibrium occurs (inflection point at which the target component adsorption amount changes for the adsorbent) is shown in FIG. 2, wherein the concentration of the extract of Moringa oleifera Lamarck added to Na-Mont per gram is about 1,200. In order to produce an optimum organic-inorganic complex at about ppm, it is necessary to add more than 1,200 ppm of horseradish (Moringa oleifera Lamarck) seed extract per Na-Mont.

       Experimental Example 1 layered aggregation and antimicrobial organic-inorganic complex turbidity removal experiment

       1-1 Artificial Wastewater Manufacturing

      Prepared by dissolving 20 g of kaolin in 1 L of distilled water and diluting it so that the turbidity is about 100 NTU.

    Here, NTU, a unit of turbidity, is an abbreviation of Nephelometric Turbidity Unit, and refers to a unit for measuring turbidity using a nephelometer. Typically drinking water is 0.5 NTU and tap water is 1.0 NTU.

      1-2 coagulation test

      Jar test was performed as follows.

      Rapid mixing (150rpm, 2min) → Slow mixing (50rpm, 15min) → Precipitation (30min)

      1-3 Experimental Results

     The sample used in the experiment was made of artificial wastewater with turbidity of about 100 NTU, and the organic-inorganic complex was injected differently in the range of 0-30 ml / L as a flocculant to evaluate the turbidity removal efficiency.

     As shown in FIG. 3, the turbidity removal efficiency of the turbidity removal efficiency was 64% (110 → 40 NTU) and 75% (110 → 28 NTU) at 20ml / L and 30ml / L. Even after time, there was no big difference.

      Experimental Example 2 layered aggregation and antimicrobial organic-inorganic complex storage experiment

      After storage for 6 months at room temperature for comparison, a) 5g of seed extract powder extracted by adding NaCl in 20ml distilled water b) Moringa oleifera Lamarck seed press cake by adding NaCl 5 g of extract powder was dissolved in 20 ml of distilled water. C) 5 g of organic-inorganic complex prepared from seeds was dissolved in 20 ml of distilled water. D) 5 g of organic-inorganic complex prepared from seed press cake was dissolved in 20 ml of distilled water. The results as shown in Table 1 below were obtained.

division enemy a) seed NaCl
Extract (20 mg / l) b) cake NaCl
Extract (20 mg / ml) c) seed + montmorillonite (20 mg / ml) d) cake + montmorillonite (20mg / ml)
30 minutes
after Turbidity
(NTU)
18.5
13.67
1.67
1.55
1.48 TOC
(mg / L)
3.7
2.57
2.34
1.93
1.89
2 hours later Turbidity
(NTU)
19.2
14.79
1.88
1.58
1.54 TOC
(mg / L)
3.9
2.68
2.87
1.87
1.93

    Here, NTU, a unit of turbidity, is an abbreviation of Nephelometric Turbidity Unit, and refers to a unit for measuring turbidity using a nephelometer. Typically drinking water is 0.5 NTU and tap water is 1.0 NTU.

      TOC stands for Total Organic Carbon, which is the amount of carbon in organic matter in water or mud.

      In this Experimental Example 2, the extracted powder of a) and the powder of b) were stored in the paste state at room temperature for 6 months so that no dust was introduced, but stored under the same conditions as the material left uncovered with a lid in a loose state. As a result of the comparative test of the composites, there was a slight difference in treatment efficiency as shown in the above results, but samples a) and b) turned brown compared to c) and d), and apparent problems were observed for use in water treatment. The organic-inorganic complexes of c) and d) were relatively unchanged, indicating that the minerals in the lattice minerals extended the shelf life due to the far infrared and negative ions.

       Experimental Example 3: layered aggregation and antimicrobial organic-inorganic complex antibacterial experiment

       Sample: sewage collection from sewage pipe (E. coli secured)

       Experimental method: As shown in Figure 4, viable count (Viablecount, cfu / ml) measurement, 3M film medium

       Experimental results: As shown in FIG.

                 E. coli strain (without organic-inorganic complex) in sewage 5 × 104 CFU / ml,

                 Organic / Inorganic Complex Addition (2g / 1ml): 5,800 CFU / ml

                 Identification of antibacterial effects by organic-inorganic complex

      Experimental Example 4 layered aggregation and antimicrobial organic-inorganic complex dyeing wastewater treatment efficiency test

      Raw water characteristics collected from Daegu Dye Wastewater Complex are shown in Table 2 below.

division Turbidity (NTU) Chromaticity (ptco) COD (mg / L) Dye Wastewater 240 1220 580

     ○ Coagulant: Organic-inorganic complex, iron salt, and inorganic polymer coagulant according to the process of 1-4 of Example 1 (The amount of organic-inorganic complex added 1.5g (1,800ppm pure seed extract equivalent) dissolved in 20ml distilled water)

      ○ The coagulation test conditions are shown in Table 3 below.

division Iron salt
(ppm) Organic-inorganic complex
(1.5g / 20ml) PAC
(ml) pH Remarks Early Iron salt injection Inorganic and inorganic complex input
One
1,400
20
-
5.00
2.94
4.06 pH adjustment
Sulfuric acid,
NaOH 2 1,400 20 - 11.35 5.00 6.24 3 700 - 0.4 5.00 3.7 6.1 4 700 20 - 5.00 3.7 6.2

     ○ Jar test was performed as follows.

       -Rapid mixing (150rpm, 2min) → Slow mixing (50rpm, 15min) → Precipitation (30min)

     ○ Experimental results

      In general, when the dyeing wastewater is treated by chemical coagulation, an inorganic polymer coagulant (polymer) is used as an iron salt and a coagulant as coagulant. In this experiment, in order to confirm the cohesive performance of the organic-inorganic complex, the iron salt injection concentration was fixed at 1,400 ppm, and comparative experiments were performed on the coagulation removal efficiency according to the addition of the organic-inorganic composite and organic polymer coagulant (PAC).

       When the inorganic polymer is injected, as shown in Table 4 below, turbidity, chromaticity, and COD removal efficiency ((initial value-final value) / percentage of initial value) are 51% (240 → 118 NTU) and 48% (1220 → 632 ptco ), 40% (580 → 345 mg / L). In contrast, turbidity, color, and COD removal efficiencies were 83% (240 → 41 NTU), 69% (1220 → 374 ptco), and 58% (580 → 242 mg / L). It was confirmed that the removal efficiency was improved by about 18 to 30% for each item, and thus the performance of the organic-inorganic complex coagulant was confirmed. The experimental results for the cohesive performance of the organic-inorganic complex are shown in Table 4 below.

division Turbidity (removal rate) Chromaticity (removal rate) COD (removal rate) Iron salt + PAC 118 (51%) 632 (48%) 345 (40%) Iron salt + organic-inorganic complex 41 (83%) 374 (69%) 242 (58%)

      Here, COD is chemical oxygen demand, which is an abbreviation of Chemical Oxygen Demand, and the value is reduced to be clean water.

      pH influence

     The coagulant used 700 ppm of iron salt and 2 g of organic-inorganic complex. In order to examine the effect of pH on the coagulation, comparative experiments were performed by varying the pH concentration to 5 and 11, as shown in Table 5 below. Removal efficiency was not significantly different.

division Turbidity (removal rate) Chromaticity (removal rate) COD (removal rate) pH 5 20 (91%) 281 (76%) 220 (62%) pH 11 35 (85%) 331 (72%) 232 (60%)

      Table 6 below shows the turbidity, chromaticity, and COD of each phase.

division Turbidity (removal rate) Chromaticity (removal rate) COD (removal rate) Remarks enemy 240 1220 580 - 1 (iron salt + organic-inorganic complex) 20
(91%) 281
(76%) 220
(62%)
1 icons 2 (iron salt + organic-inorganic complex) 35
(85%) 331
(72%) 232
(60%)
2 icons 3 (iron salt + PAC) 118
(51%) 632
(48%) 345
(40%)
3 icons 4 (iron salt + organic-inorganic complex) 41
(83%) 374
(69%) 242
(58%)
4 icons

      Experimental Example 5: Layered Aggregation and Antibacterial and Inorganic Complex Deodorization Test

      The porous material used by the present invention is generally used as a deodorant, an absorbent, and the like, and is particularly used as an animal feed additive. Therefore, the novel organic / inorganic complex of the present invention may be used for the same purpose, and thus manufactured Na-Mont. To carry out the deodorization test of the complex was prepared a feed as shown in Table 7.

      Feed composition ingredient Test Example Control Boiled minced pork meat 50 50 rice 30 30 General feed 12 12 water 5 5 Organic-inorganic complex 3 0

      The prepared feed was fed to two-year-old poodles (average body weight 3kg) at 30 g / day, and then feces were collected and the smell was examined to determine the difference in smell. The results obtained are summarized in Table 8 below.

      Fecal odor was evaluated and evaluated in 5 steps from the weakest odor 1 to the strong odor 5.

      Odor rating Date Types of Cattle Feed Poodle number one Poodle number 2 1 day Control 4 4 2 days Control 5 5 3 days Control 5 5 4 days Test group 3 3 5 days Test group 2 2 6 days Test group 2 One 7 days Test group 2 2

      Experimental Example 5 Comparison of Before and After Characteristics of Test Group Using Cationic Peptides Extracted from Horseradish Tree Seeds

      Bacterial reduction rate was tested against Staphylococcus aureus ATCC 6538 and Klebsiella pneumoniae ATCC 15442 using cationic peptides prepared by 1-2- (1) of Example 1.

    ○ Pneumococcal killing effect

    end. Test strain: Staphylococcus aureus ATCC 6538

    I. Test Method: KICM-FIR-1002

    All. Test Result: As shown in FIG.

                 In the comparative example (BLANK), the number of bacteria increased after 24 hours, and in the case of using the cationic peptide of the present invention (test example), the number of bacteria decreased after 24 hours and showed a 99.8% bacterial reduction rate.

    ○ Pneumococcal killing effect

    end. Test strain: Klebsiella pneumoniae ATCC 15442

    I. Test Method: KICM-FIR-1002

    All. Experimental results: As shown in Figure 8,

              In the comparative example (BLANK), the number of bacteria increased after 24 hours, and in the case of using the cationic peptide of the present invention (test example), the number of bacteria decreased after 24 hours and showed a 99.8% bacterial reduction rate.

    The results of the bacterial reduction rate test for Pseudomonas aeruginosa and pneumococci using cationic peptides prepared by 1-2- (1) of Example 1 are shown in Table 9 below.

      Bacterial killing effect
Test Items Test result
Test Methods
Initial concentration
( CFU / 40p) 24 hours later
 density
( CFU / 40p)
Bacterial reduction rate
(%)
Antibacterial test by P. aeruginosa BLANK 401 2734 -

KICM - FIR
-1002:
2006 Cationic Pepta Id extract
use
401 One
99.8
Antibacterial test by pneumococcal BLANK 420 2831 - Cationic Pepta Id extract
use
420
One
99.8

      Example 2 porous coagulation and antimicrobial organic-inorganic composite manufacturing process

     After dissolving 2 g of the plant extract dried powder obtained in Example 1-1 in 50 ml of distilled water, 10 g of activated carbon powder was added instead of Na-Mont used in Example 1-3, stirred for 1 hour with a stirrer, and dried in an 80 oven for 24 hours. Organic-inorganic activated carbon composite was prepared.

     After dissolving 2 g of the plant extract dried powder obtained in Example 1-1 in 50 ml of distilled water, 10 g of kaolin balls having an average particle diameter of 0.5 mm were added instead of Na-Mont used in Example 1-3, followed by stirring for 12 hours with a stirrer, followed by 80 Drying in an oven for 24 hours to prepare an organic-inorganic kaolin ball complex.

      2 g of the plant extract dried powder obtained in Example 1-1 was dissolved in 50 ml of distilled water, and then 10 g of Na-Zeolite zeolite was added instead of Na-Mont used in Example 1-3, followed by stirring in a shaker for 12 hours, followed by an oven at 80 ° C. After drying for 24 hours to prepare an organic-inorganic zeolite composite.

      Jar Tset was carried out according to the method shown in Example 1, respectively, to obtain the results shown in Table 10 below. For reference, this test was carried out only with the naked eye test, and in all three materials having excellent adsorptivity when used alone as shown in FIG. Verified.

material change Activated Carbon Inorganic Complex Clearly settled Kaolin Ball Organic / Inorganic Complex Clearly settled Zeolite Organic Inorganic Complex Clearly settled

      The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

1 is a schematic diagram showing a manufacturing process of the organic-inorganic composite according to the present invention.

2 is a graph showing the adsorption amount of the organic-inorganic complex according to the present invention.

Figure 3 is a graph showing the turbidity after 5 hours of precipitation according to the organic-inorganic complex content according to the present invention.

Figure 4 is a view showing the antimicrobial test method of the organic-inorganic complex according to the present invention.

Figure 5 is a photograph showing the antimicrobial test results of the organic-inorganic complex according to the present invention.

Figure 6 is a photograph showing the aggregation test results of the organic-inorganic complex according to the present invention.

Figure 7 is a photograph showing the results of killing Pseudomonas aeruginosa using cationic peptide powder according to the present invention.

Figure 8 is a photograph showing the pneumococcal killing results using the cationic peptide powder according to the present invention.

Claims (16)

Cationic peptides containing two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from residual press cakes extracted from oil of Moringa oleifera Lamarck seeds or horseradish seeds A cation and antimicrobial organic-inorganic complex characterized in that the cationic peptide 300 ~ 600mg is contained in 1g of the layered silicate mineral by ion exchange with the metal cation of the layered silicate mineral. Cationic peptides containing two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine or arginine extracted from residual press cakes extracted from oil of Moringa oleifera Lamarck seeds or horseradish seeds Adsorbed to a layered silicate mineral from which metal cations have been removed; and 300 to 600 mg of the cationic peptide is trapped and contained in 1 g of the layered silicate mineral. Cationic, containing two or more amino acids of the same or different amino acids of glutamic acid, proline, methionine, or arginine extracted from the residual press cake extracted from oil of Moringa oleifera Lamarck seeds or horseradish seeds Peptide; a porous material capable of adsorbing a cationic peptide; by adsorbing, the cationic peptide 300 ~ 600mg is contained in 1g of the porous material, characterized in that the organic-inorganic complex for aggregation and antibacterial. The method according to claim 1 or 2, wherein the metal cation is Na ⁺ , K ⁺ , Mg ^{2 +} , Aggregate and antimicrobial organic-inorganic complex characterized in that the Ca ^{2 +} or Al ^{3 +} ion. According to claim 3, The porous material is charcoal, activated carbon, glass beads, kaolin ball, volcanic clavicle rock, silica, alumina or titania, characterized in that the organic-inorganic composite for antibacterial and antibacterial. Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder; Immersing the seed powder in n-hexane and stirring to remove oil; Separating the solid and the supernatant by filtration with filter paper after removing the oil; Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities; Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) to 80 to 100 ° C. until the pH is 7.5 to 8.5 in the remaining filtrate to form an aggregated active aqueous solution; Concentrating the coagulated active aqueous solution under reduced pressure with a rotary vacuum evaporator and freeze drying to form a dry powder; And Distilled water is added to the dry powder to form an cationic peptide powder by separating sodium ions by electrophoresis to form a cationic peptide powder. Peeling and grinding the horseradish (Moringa oleifera Lamarck) plant seeds to make seed powder, extracting oil, and then making a seed cake powder; Dipping the seed cake powder in ether and then stirring to remove oil; Separating the solid and the supernatant by filtration with filter paper after removing the oil; Adding distilled water to the solid and then stirring the filtrate with a filter paper to remove impurities; Removing the impurities and stirring the sodium bicarbonate (NaHCO ₃ ) to 80 to 100 ° C. until the pH is 7.5 to 8.5 in the remaining filtrate to form an aggregated active aqueous solution; Pressure-concentrating the flocculent active aqueous solution with a rotary vacuum evaporator and then freeze drying to form a dry powder; And Distilled water is added to the dry powder to form an cationic peptide powder by separating sodium ions by electrophoresis to form a cationic peptide powder. Powder production step of making horse seed powder (Moringa oleifera Lamark) plant seeds, peeling and grinding the seed powder; An oil removal step of removing oil contained in the seed powder; An eluting step of eluting the extracted peptide in an aqueous solution of sodium bicarbonate (NaHCO ₃ ) by extracting the peptide from the powder obtained through the oil removal step; A step of obtaining an agglomerated active solution to remove the impurities of the aqueous solution which has passed through the elution step to obtain an agglomerated active solution; A peptide concentration and separation step of concentrating and separating only peptides from the aggregation active aqueous solution which has undergone the step of obtaining the aggregation active aqueous solution; Purification step of removing the residual anionic material by using a cationic exchange resin column to capture only the pure cationic peptide to the obtained product after the peptide concentration and separation step: Cationic peptide extraction method comprising a; powder forming step of forming a powder from the obtained product after the purification step. The method of claim 8, wherein the step of removing oil, 100 ~ by immersing the seed powder in one or a mixture of alcohol and n-hexane as an organic solvent in a ratio of 1: 2 to the weight of the pressing method or a weight ratio; After stirring 20 to 30 minutes at a speed of 200rpm and put in a drying pan to completely remove any components of the organic solvent alcohol and n-hexane or a mixture thereof and put in an oven at a temperature of 10-40 degrees Celsius Method of evaporating the organic solvent by leaving it for 24 to 48 hours; oil removal step of removing the oil by repeating any one of the process 3 to 5 times, In the elution step, the powder obtained through the oil removal step is put into an aqueous solution of sodium bicarbonate (NaHCO ₃ ) having a concentration of 1 M per 100 g of powder, and heated at a temperature of 50 to 60 ° C. in a 1 L aqueous solution adjusted to pH 7.5 to 8.5. Eluting the peptide in an aqueous solution through agitation, The step of obtaining the coagulant active aqueous solution, step of obtaining a coagulant active aqueous solution comprising the step of removing impurities through the filter paper, The step of concentrating and separating the peptide may be a step of concentrating and separating the peptide through an cellulose membrane or an ultrafiltration process of 14 kDa or less of the coagulant active aqueous solution obtained through the step of obtaining the coagulant active aqueous solution, The purifying step may include purifying the obtained product after the concentration and separation step by passing a cationic exchange resin column at a rate of 5 ml / min for 36 to 48 hours to remove anionic material; Wherein the powder forming step, the cationic peptide extraction method comprising the powder forming step of forming a seed powder by freeze-drying the obtained product after the purification step under reduced pressure at a temperature of minus 80 degrees for 24 to 48 hours.  The cationic peptide powder produced by the cationic peptide extraction method in any one of Claims 6-9. Dispersing the cationic peptide powder of claim 10 in deionized water; Dipping the layered silicate mineral in deionized water to remove metal cations bound in the lattice structure using ultrasonic waves; Forming a peptide-mineral adsorbate by stirring and adsorbing the cationic peptide powder dispersed in the deionized water and the layered silicate mineral from which the metal cation has been removed for 18 to 48 hours; And Filtering the peptide-mineral adsorbate and removing moisture, followed by drying in an oven at 70 to 100 ° C. for 18 to 36 hours or freeze drying under reduced pressure to form an organic-inorganic complex; Way. A flocculant containing the flocculation and antimicrobial organic-inorganic complex as described in any one of Claims 1-3 as an active ingredient. An adsorbent containing the aggregated and antimicrobial organic-inorganic complex according to any one of claims 1 to 3 as an active ingredient. A deodorant containing the flocculation and antimicrobial organic-inorganic complex according to any one of claims 1 to 3 as an active ingredient. A food additive comprising the aggregation and antimicrobial organic-inorganic complex according to any one of claims 1 to 3 as an active ingredient. Toothpaste containing the aggregation and antimicrobial organic-inorganic complex as described in any one of Claims 1-3 as an active ingredient.

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