KR100934286B1 - Polymorphic Markers for Osteoporosis Prediction and Osteoporosis Prediction Method Using the Same - Google Patents

Abstract

The present invention relates to a polymorphic marker for osteoporosis prediction, a haplotype marker, and a method for predicting osteoporosis using the same. Specifically, the present invention relates to osteoporosis by identifying polymorphisms and haplotypes in the interleukin (IL) -15 gene, which is associated with bone density. It relates to how to predict and diagnose risk. The method of the present invention can predict the risk of developing osteoporosis and can be usefully used in the field of treatment for metabolic bone disease.

Osteoporosis, interleukin (IL) -15, prediction method, prediction kit.

Description

A polymorphic marker of prediction of osteoporosis and the prediction method of osteoporosis using the same}

The present invention relates to a polymorphic marker for osteoporosis prediction, a haplotype marker, and a method for predicting osteoporosis using the same, and more specifically, by identifying polymorphisms and haplotypes in the interleukin (IL) -15 gene that is associated with bone density. The present invention relates to a method for predicting and diagnosing the risk of osteoporosis.

Osteoporosis is a systemic bone disease characterized by low bone mineral density (BMD) and increased fracture risk. Osteoporosis is affected by multiple risk factors, including genetic factors, nutrition, hormones and medications. Genetic factors play an important role, and twin studies have shown that about 50-86% of the mutations in BMD are equivalent (Arden NK & Spector TD, J Bone Miner Res 12 (12): 2076-81, 1997; Ralston SH & de Crombrugghe B, Genes Dev 20 (18): 2492-506, 2006; Slemenda CW et al. , J Bone Miner Res 6 (6): 561-7, 1991; Lander ES & Schork NJ, Science 265 (5181): 2037-48, 1994; Stewart TL & Ralston SH, J Endocrinol 166 (2): 235-45, 2000). Therefore, it is important to identify (identify) a gene that acts as a regulator of BMD, which may be used to identify and protect a subject at risk for osteoporosis. To date, vitamin D receptor (VDR; vitamin D receptor), estrogen receptor (ER), transforming growth factor beta 1 (TGFB1; transforming growth factor beta 1), interleukin-1 receptor antagonist (IL1RA; Interleukin-1 receptor) antagonist), interleukin 6 (IL6), collagen 1A1 (COL1A1; collagen, type I, alpha 1), osteoprotegerin (OPG) and OSCAR (Korea Patent Publication 10-2006-0120390) Polymorphisms in genes have been reported as genes associated with bone mass.

Low bone mass is due to an imbalance in bone remodeling regulated by bone resorption of osteoclasts and bone formation of osteoblasts. Therefore, the study of the genetic effect of osteoclast or osteoblast function on the pathogenesis of osteoporosis is very important. Human IL-15 , consisting of 10 exons, is located on chromosome 4q31 and is involved in the regulation of inflammation and pathological bone destruction, and is a pleiotropic inflammatory cytokine that plays a role in the pathogenesis of rheumatoid arthritis (RA). To perform. The IL-15 mRNA and protein are increased in the synovial membrane and serum of patients with RA and psoriatic arthritis (PsA). IL-15 on the cell surface was detected in T cells and monocytes isolated from peripheral blood and joint synovial fluid (SF) in RA patients (Miranda-Carus ME et al. , Arthritis Rheum 54 (4): 1151-64 , 2006), administration of water soluble murine IL-15 Rα inhibited the development of collagen-induced arthritis (Ruchatz H et al. , Immunol 160 (11): 5654-60, 1998). The results suggest that IL-15 mediates inflammatory bone destruction. IL-15 is also involved in the formation of osteoclasts. That is, IL-15 has been reported to promote the formation of multinuclear osteoclast-like cells in rat bone marrow cultures (Ogata Y et al ., J Immunol 162 (5): 2754-60, 1999). IL-15 has also been shown to promote the production of TNF-α in osteoclast precursors. However, the promotion of multinucleated osteoclast-like cell formation by IL-15 was not eliminated by administration of TNF-α neutralizing antibody. In contrast, neutralization of IL-15 inhibited the differentiation of osteoclasts in T cells and monocyte cultures derived from articular synovial fluid of RA patients (Miranda-Carus ME et al. , Arthritis Rheum 54 (4): 1151-64, 2006 ). The results suggest that IL-15 actively promotes the differentiation of osteoclasts through TNF-α-independent methods and that IL-15 gene mediates the regulation of bone metabolism and mediates the pathogenesis of osteoporosis in addition to RA.

In this regard, the present inventors have identified four single nucleotide polymorphisms of the IL-15 gene (SNPs; + 20A> G, + 13467C> A, in BMD of several bone regions including lumbar spine and femur in Korean postmenopausal women). + 13653A> T and + 13815A> T) and two haplotypes (ht1 and ht2) were identified to complete the present invention.

It is an object of the present invention to provide a polymorphic marker for osteoporosis prediction, a haplotype marker, and a method for predicting osteoporosis using the same.

In order to achieve the above object, the present invention is selected in the interleukin (hereinafter referred to as IL ) -15 genomic gene having SEQ ID NO: 15, and the 20th base from the translation start point (85265 base of SEQ ID NO: 15) is G. The present invention provides a polymorphic marker for predicting osteoporosis composed of polynucleotides consisting of 20 to 100 consecutive nucleic acid molecules including the +20 th base.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13467 base is A from the translation start point (85265 base of SEQ ID NO: 15), and 20 to 20 including the +13467 base Provided is a polymorphic marker for osteoporosis prediction consisting of polynucleotides consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13653 base is T from the translation start point (85265 base of SEQ ID NO: 15), 20 ~ 20 including the +13653 base Provided is a polynucleotide associated with osteoporosis consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13653 base is T from the translation start point (85265 base of SEQ ID NO: 15) and 20 comprising the +13653 base Provided are polymorphic markers for predicting osteoporosis consisting of polynucleotides consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13815 base is T from the translation start point (85265 base of SEQ ID NO: 15), 20 ~ 20 including the +13815 base Provided is a polymorphic marker for osteoporosis prediction consisting of polynucleotides consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, +20 base G, +13467 base C, +13653 base from the translation start point (85265 base of SEQ ID NO: 15) Provides A, a haplotype marker for osteoporosis prediction with a + 13815th base T.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, +20 base A, +13467 base A, +13653 base from the translation start point (85265 base of SEQ ID NO: 15) Provides a haplotype marker for predicting osteoporosis in which T, +13815 base is A.

In addition, the present invention

1) extracting genomic DNA from blood samples isolated from the subject;

2) detecting at least one polymorphic site of said IL-15 polymorphic marker present on genomic DNA of step 1); And

3) A method for predicting osteoporosis, comprising comparing the detection result of step 2) with the detection result of a polymorphic site of a normal person.

In addition, the present invention provides a kit for predicting osteoporosis comprising a primer pair capable of amplifying a polymorphic marker of the IL-15 gene.

Hereinafter, the present invention will be described in detail.

The present invention is selected within the interleukin ( IL ) -15 genomic gene having SEQ ID NO: 15, the +20 base is G from the translation start point (85265 base of SEQ ID NO: 15), and the +20 base It provides a polymorphic marker for osteoporosis prediction consisting of polynucleotide consisting of 20 to 100 consecutive nucleic acid molecules including.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13467 base is A from the translation start point (85265 base of SEQ ID NO: 15), and 20 to 20 including the +13467 base Provided is a polymorphic marker for osteoporosis prediction consisting of polynucleotides consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13653 base is T from the translation start point (85265 base of SEQ ID NO: 15), 20 ~ 20 including the +13653 base Provided is a polynucleotide associated with osteoporosis consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13653 base is T from the translation start point (85265 base of SEQ ID NO: 15), 20 ~ 20 including the +13653 base Provided are polymorphic markers for predicting osteoporosis composed of polynucleotides consisting of 100 consecutive nucleic acid molecules.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, the +13815 base is T from the translation start point (85265 base of SEQ ID NO: 15), 20 ~ 20 including the +13815 base Provided is a polymorphic marker for osteoporosis prediction consisting of polynucleotides consisting of 100 consecutive nucleic acid molecules.

The present inventors examined the age, weight, height, and postmenopausal periods of healthy postmenopausal women who visited Asan Medical Center, and then measured the BMD of the lumbar spine and femoral neck. Observation of significant correlation with the postoperative period confirmed that the subjects of this study were suitable for studying osteoporosis (see Table 1).

Then, DNA was extracted from the peripheral blood leukocytes of the test subject, and gene polymorphism was examined through sequencing of the exon region, its boundaries, and the promoter region of the IL-15 gene. As a result, a total of 16 single nucleotide polymorphisms, two at the promoter site, nine at the 5 'untranslated regions (UTRs) at the intron site, and four at the 3' untranslated site ) (See FIG. 1). Six of these SNPs were in an associative imbalance (absolute linkage disequilibrium, | D '| = 1 and r ² = 1). Of the 16 polymorphisms, five common polymorphic markers (-156T> C, + 20A> G, + 13467C> A, + 13653A> T and + 13815A> T) were assigned to minor allele frequencies (MAF> 0.1) and LD values. Were selected for large scale genotyping (n = 844) based on the analysis, and the five genotype distributions selected were in the Hardy-Weinberg equilibrium (see Table 3).

For the subjects, the sequence of the five selected polymorphisms, bone density of the lumbar spine and femoral neck, and fractures of the spine and non-vertebrae were examined to determine the IL-15 gene polymorphism, bone density and fractures of the spine and non-vertebrae. The correlation was investigated. As a result, four SNPs (+ 20A> G, + 13467C> A, + 13653A> T and + 13815A> T) and two haplotypes (ht1 and ht2) were found to be associated with low BMD levels and risk of osteoporosis It became. In addition, these results are the first clinical reports suggesting the genetic role of IL-15 polymorphism in bone metabolism, and the + 20A> G, + 13467C> A, + 13653A> T and + 13815A> T of IL-15 genes. Polymorphic markers have been described as a genetic factor of osteoporosis in postmenopausal women.

However, because osteoporosis is characterized as a low BMD, analysis of the genetic impact of fractures of four SNPs and two haplotypes revealed that osteoporotic fractures, despite significant correlations with low BMD levels in the lumbar spine and thigh There was no association with increased risk (no data). This suggests that many other environmental factors play an important role in determining fracture risk. Indeed, the genetic probability of osteoporosis risk was estimated at 25-35% (Deng HW et al. , J Bone Miner Res 15 (7): 1243-52, 2000; MacGregor A et al. , Bmj 320 (7250): 1669- 70; author reply 670-1, 2000), which is very low compared to BMD values (Eisman JA, Endocr Rev 20 (6): 788-804, 1999; Peacock M et al. , Endocr Rev 23 (3). ): 303-26, 2002). However, the fact that + 13467C> A, + 13653A> T and ht2 are not related to fracture risk cannot rule out their role as genotype markers in bone metabolism.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, +20 base G, +13467 base C, +13653 base from the translation start point (85265 base of SEQ ID NO: 15) Provides A, a haplotype marker for osteoporosis prediction with a + 13815th base T.

In addition, the present invention is selected within the IL-15 genomic gene having SEQ ID NO: 15, +20 base A, +13467 base A, +13653 base from the translation start point (85265 base of SEQ ID NO: 15) Provides a haplotype marker for predicting osteoporosis in which T, +13815 base is A.

We calculated 99% of the haplotype observed by calculating the phase probability for the haplotype of the four + 20A> G, + 13467C> A, + 13653A> T and + 13815A> T polymorphic markers of the present invention. Four common haplotypes (ht1 to ht4; frequency> 0.05) were identified (see FIG. 2), and genotypes were analyzed in postmenopausal Korean women (n = 844) using the four polymorphic markers. Results: ht1 (GCAT: + 20G, + 13467C, + 13653A, and + 13815T) and ht2 (AATA: + 20A, + 13467A, + 13653T, and + 13815A) haplotypes showed lumbar spine in the co-dominant and dominant models (p = 0.01 And 0.02-0.005) and femoral neck (p = 0.002-0.005 and 0.002-0.001) were significantly associated with BMD levels (see Table 5).

In addition, the present invention

1) extracting genomic DNA from blood samples isolated from the subject;

2) detecting at least one polymorphic site of said IL-15 polymorphic marker present on genomic DNA of step 1); And

3) A method for predicting osteoporosis, comprising comparing the detection result of step 2) with the detection result of a polymorphic site of a normal person.

In the above method, the polymorphic site detection method of step 2) includes sequencing analysis, hybridization by microarray, allele specific PCR, dynamic allele-specific hybridization method, and DASH. ), PCR extension analysis or TaqMan technique.

In addition, the present invention provides a kit for predicting osteoporosis comprising a primer pair capable of amplifying a polymorphic marker of the IL-15 gene.

The kit comprises a primer pair capable of amplifying the + 20A> G, + 13467C> A, + 13653A> T and + 13815A> T polymorphic markers of the IL-15 gene, wherein the primer pair is preferably + 20A> PCR products using the primer pairs comprising two polynucleotides set forth in SEQ ID NOS: 1-6 as described in Table 2 for G, + 13467C> A, + 13653A> T and + 13815A> T polymorphic markers The osteoporosis can be predicted by confirming the sequence of the four polymorphic sites obtained by analyzing the nucleotide sequence. The primer pair is not limited in size and position to bind to the template as long as it can amplify the polymorphic site, and those skilled in the art can easily design the primer using conventional primer selection software.

As described above, the + 20A> G, + 13467C> A, + 13653A> T, and + 13815A> T polymorphic markers and haplotype markers of the IL-15 gene are closely related to bone mineral density. It can be usefully used to prevent this.

Hereinafter, the present invention will be described in detail by way of examples. However, the following Examples are only for illustrating the present invention and do not limit the present invention.

Example 1 Selection of Test Subject

<1-1> selection criteria

The subjects were 844 healthy postmenopausal women who visited Asan Medical Center (AMC). Among them, women who were premenopausal before the age of 40, women who received drugs that affect bone metabolism more than 6 months or less than 12 months, and women who had diseases affecting bone metabolism were excluded. In addition, women who had previously experienced dementia or stroke were also excluded due to limited physical activity. Severe facet joint osteoarthritis in the lumbar spine, diagnosed by female and / or general spinal radiographs of the fourth grade or higher of Nathan's classification (Nathan et al. J Bone Joint Surg Am 44A: 243-268, 1962). Women with) were also excluded. The present invention has been approved by the AMC Ethics Review Committee, and all subjects have submitted a Consent Statement.

<1-2> Bone Mineral Density in the Lumbar Spine (L2-L4) and Femoral Neck

BMD (g / cm ² ) was measured at the lumbar region (L2-L4) and femoral neck using a dual-energy X-ray absorptiometry. Specifically, 560 women were measured using Lunar Expert XL scanner (Lunar, USA) of software version 1.90, and 284 women were measured using Hologic QDR 4500-A scanner (Waltham, USA) of software version 4.84. The subject was measured. Because of the upper-extremity dominance, BMD in the proximal femur was measured in the non-dominant area. The short-term in vivo measurements for Lunar and Hologic devices, expressed as coefficients of variation, were 0.82 and 0.85% in the lumbar spine and 1.12 and 1.20% in the femoral neck, respectively. The figures were obtained from 17 volunteers not included in the 844. The volunteers were measured five times a day and climbed up and down the table between every measurement. To derive cross-correction between the two systems, BMD levels were measured in 109 healthy Korean women (55 ± 11 years, 31 to 75 years) by two machines at the lumbar and femoral necks. The formula was calculated as follows (Jo et al . Korean J Nucl Med 33: 282-288, 1999).

Lumbar region (L2-L4) BMD (g / cm ² ): Lunar = 1.1287 ㅧ Hologic-0.0027

Femoral neck BMD (g / cm ² ): Lunar = 1.1556 ㅧ Hologic + 0.0182

Clinical aspect Clinical aspect BMD (g / cm ² ) Lumbar region Femoral neck a constant Mean ± standard deviation β SE P β SE P Age (years) 58.81 ± 7.52 -0.005 0.002 0.0010 -0.003 0.001 0.009 Weight (kg) 56.30 ± 7.18 0.005 0.001 <0.001 0.004 0.001 <0.001 Height (cm) 155.09 ± 5.28 0.002 0.001 0.05 0.001 0.001 0.45 Postmenopausal period (years) 9.48 ± 7.80 -0.004 0.001 0.009 -0.004 0.001 0.001 Calibrated R ² = 0.2319 Calibrated R ² = 0.2037

As a result, the correlation between the clinical profile and post-menopausal women, BMD and age, year since menopausal (YSM), weight and height were as shown in Table 1. The average age of the participants was 58.81 ± 7.52 years and the mean YSM was 9.48-7.8 years (Table 1). BMD was measured using two machines: Lunar Expert XL (560, 66.35%) and Hologic QDR 4500-A (284, 33.65%). The BMD value as measured by Lunar device (lumbar spine and each of 0.887 ± 0.145 and 0.734 in the femoral neck ± 0.105 g / ㎝ ²⁾ is a value measured by Hologic device (in the lumbar spine and femoral neck, respectively 0.785 ± 0.092 and 0.608 ± 0.067 g / ㎝ ² ; p <0.0001). However, after applying the cross-correction equation, there was no significant difference (no data). In addition, as expected, age, weight and YSM were found to be significantly associated with BMD of the lumbar and thigh. Age and YSM were negatively correlated with BMD, and body weight was positively correlated with BMD. Kidneys showed a significant positive correlation with lumbar BMD. In other words, age, weight, height and postmenopausal periods were significantly correlated with BMD of lumbar spine and femoral neck.

<1-3> Bone Mineral Density at Other Sites

In addition, BMD levels were measured in other proximal femur regions including Ward triangle, trochanter, femoral shaft, and total femur. In femoral livers, BMD was not measured using a Hologic machine. BMD levels in the femoral and other proximal femur regions were valid only in 331 and 460 patients, respectively. Relevance of BMD levels and IL-15 gene was determined through statistical adjustment using the machine as a covariate (no data). This is because cross-correction data were not available in this region of Korean women.

<1-4> Detection of spinal and non-vertebral fractures

Evaluation of vertebral fractures was performed according to the recommendations of the Working Group on Vertebral Fractures (Kiel D, J Bone Miner Res 10 (4): 518-23, 1995; Orimo H et al ., J Bone Miner) Metab 19 (6): 331-7, 2001). Lateral radiographs of the chest tube and lumbar spine (T4 to L4) were taken using a standard X-ray apparatus. Radiographs were evaluated at AMC by expert radiologists who did not know about the present invention. By quantitatively defining vertebral fractures as 15% of vertebrae are injuries (compared with intact data from previous experiments) or a reduction of 4 mm in measured verebral height (front, middle, or posterior), Radiographic evaluations were performed using visual semiquantitative techniques in patients without previous severe trauma such as traffic accidents (no data). In addition, the incidence of non-vertebral fractures (wrist, hip, forearm, upper arm, rib and pelvis) was assessed by self-report in patients over 50 years of age. Patients with fractures caused by severe trauma, such as obvious car accidents or falls at heights above the kidneys, were excluded.

As a result, spinal and non-vertebral fractures were indicated in 115 and 96 patients, respectively.

<Example 2> IL-15 Gene polymorphism analysis

<2-1> Sequencing

The present inventors extracted DNA from healthy Korean peripheral blood leukocytes selected by the method of Example 1 using a genome DNA extraction kit (Wizard genomic DNA purification kit, Promega, USA) according to the manufacturer's instructions. Gene polymorphisms were examined through sequencing using a DNA sequence analyzer (ABI PRISM 3730 DNA analyzer, Applied Biosystems, USA) at all exon sites and their boundaries and promoter sites (about 1.5 kb). Five primer sets for DNA amplification and sequencing were based on the GenBank database ( IL-15 NT_016354), and the primer set sequences were shown in Table 2. Sequence variation of the product obtained after PCR was confirmed by each chromatogram.

As a result, as shown in Fig. 1, two in the promoter region, nine in the intron region, one in the 5 'untranslated regions (hereinafter referred to as UTRs) and four total in the 3' untranslated region Sixteen single nucleotide polymorphisms (SNPs) were identified. The frequency of polymorphism in the postmenopausal female population in Korean is shown in parentheses.

Primer Pairs for SNP Identification Seat location primer SEQ ID NO: order -156T> C Exxon 3 Forward direction One TGCTACTAACCTTCCTCCATACC Reverse 2 GAACAATCCAAATGTGAACAGAC + 20A> G Intron 3 Forward direction One TGCTACTAACCTTCCTCCATACC Reverse 2 GAACAATCCAAATGTGAACAGAC + 13467C> A Exon 8 Forward direction 3 CTCCCTGTTCTCTTTCCTCG Reverse 4 TTTGACTATCACCCTTAAACAAATG + 13653A> T Exon 8 Forward direction 5 CAGACCTTGGATCAGATGAACTC Reverse 6 CAAAGTTGTCCTGGGCCG + 13815A> T Exon 8 Forward direction 5 CAGACCTTGGATCAGATGAACTC Reverse 6 CAAAGTTGTCCTGGGCCG

<2-2> Genetic Polymorphism Analysis

Hardy-Weinberg equilibriums (HWE) were calculated using the χ ² test to determine whether the frequency of each SNP identified by the method of Example 2 is equilibrium in individual differences at each location in the population. Lewontin's D '(| D' |) and linkage disequilibrium coefficients ( r ² ) were examined by pair wise comparison between all pairs of bialleric loci (Hedrick). PW, Genetics 117 (2): 331-341, 1987) and Haploview version 3.2 (Whitehead Institute for Biomedical Research, USA) software were used to investigate the structure of LD clusters (Barrett JC et al. , Bioinformatics 21 (2). ): 263-265, 2005). The program used the expectation maximization (EM) of two markers to measure the maximum likelihood value of the four germ frequencies derived from the D 'and LOD values.

As a result, as shown in FIG. 1, six sets of markers were revealed in absolute LD (| D ′ | = 1 and r ² = 1, FIG. 1) by pairwise comparison between all 16 polymorphisms. , Five common polymorphic markers (-156T> C, + 20A> G, + 13467C> A, + 13653A> T, and + 13815A> T) are large genotypes based on minor allele frequencies (MAF> 0.1) and LD values Selected for analysis (n = 844). The polymorphic MAFs analyzed for the five genotypes were 0.104 (-156T> C), 0.5 (+ 20A> G), 0.396 (+ 13467C> A), 0.313 (+ 13653A> T), and 0.479 (+ 13815A> T). . Genotype distributions at all loci in large-scale analyzes were in the Hardy-Weinberg equilibrium (P> 0.05). LD analysis results of the five polymorphic markers are shown in FIG. 3.

<2-3> haplotype analysis

Haplotypes (ht) of individuals are Stephens using a Bayesian approach that incorporates a priori projections of haplotype structures based on population genetics and coalescent theory. It was estimated using an algorithm developed by Stephens M et al., Am J Hum Genet 68: 978-989, 2001. Phase probability for haplotypes of all polymorphic markers was calculated for each individual using PHASE software (Stephens et al. Am J Hum Genet 68: 978-989, 2001), and individuals with phase probability less than 97% Excluded from the analysis. The genetic effect of the estimated haplotype was also analyzed in the same way as in the analysis of polymorphism.

As a result, four common haplotypes (ht1 to ht4) of four polymorphic markers (+ 20A> G, + 13467C> A, + 13653A> T and + 13815A> T) appearing in 99% of all observed haplotypes; Frequency> 0.05) (FIG. 2 and Table 3). The frequencies of the four sequence variation sites selected were 0.488, 0.454, 0.357 and 0.4868, respectively.

Genetic Polymorphism Assay Seat location rs # genotype frequency Heterojunction HWE -156T> C Exxon 3 rs1493013 T CT C N 0.056 0.106 0.083 749 95 0 844 + 20A> G Intron 3 rs2857261 A AG G N 0.488 0.500 0.667 218 428 198 844 + 13467C> A Exon 8 rs10519613 C AC A N 0.454 0.496 0.802 250 422 172 844 + 13653A> T Exon 8 new A AT T N 0.357 0.459 0.345 355 375 114 844 + 13815A> T Exon 8 rs1057972 A AT T N 0.486 0.500 0.662 220 428 196 844

Example 3 Genotyping by Fluorescence Polarization Detection

Genotyping of selected polymorphic sites was performed based on minor allele frequency (MAF> 0.1) and linkage disequilibrium (LD). The PCR primers and probes shown in Table 4 for the assay were designed using Primer Express (Applied Biosystems, USA) for MGB TaqMan (Livak KJ, Genet Anal 14 (5-6): 143-9, 1999; 4) One allele probe was labeled with FAM dye and the other was labeled with fluorescent dye VIC. PCR reactions were performed using the 900 nM PCR primers in Table 4 and the TaqMan Universal Master mix (Applied Biosystems, USA) without 200 nM TaqMan MGB-probe and uracil DNA glycosylase (UNG) It became. The reaction was performed at 50 ° C. for 2 minutes with a total reaction volume of 5 μl containing 20 ng of genomic DNA as template DNA in a 384 well plate; 95 ° C. 15 sec, 60 ° C. 1 min 40 repetitions; It was carried out in PE 9700 (Applied Biosystems, USA) at 95 ° C. for 10 minutes. Fluorescence sensitivity was measured on a Prism 7900HT instrument (Applied Biosystems, USA) and analyzed using the automation software SDS 2.1 (Applied Biosystems, USA).

Primer Pairs for Genotyping Seat location primer order SEQ ID NO: -156T> C Exxon 3 Forward direction GATGCATCTGTTAAGTAACTAATAGAGTGCTTAT 7 Reverse TCCATTAAAAACACAAGTGCACTAGTAAGA 8 Probe-1 (FAM) AGTATTAATTCCCTTCCTTTCT 9 Probe-2 (VIC) TATTAATTCCCTCCCTTTCT 10 + 20A> G Intron 3 Forward direction Private C_1204075_20 ^* Reverse Probe-1 (FAM) Probe-2 (VIC) + 13467C> A Exon 8 Forward direction Private C_1840893_10 ^* Reverse Probe-1 (FAM) Probe-2 (VIC) + 13653A> T Exon 8 Forward direction TGAACTTCTCTCAGACTTACTTTACTCATTTTTTTAAT 11 Reverse TCAGTGCAACTTAAAAAACAACACTTGT 12 Probe-1 (FAM) TGGAATAATGTAAAATGTTG 13 Probe-2 (VIC) TGGAATAATGTAATATGTTG 14 + 13815A> T Exon 8 Forward direction Private C_8976918_10 ^* Reverse Probe-1 (FAM) Probe-2 (VIC)

* Prolied by Applied Biosystems, sequence is private.

<Example 4> IL-15 Investigation of the correlation between gene polymorphism and clinical factors

<4-1> Correlation with BMD

BMD levels and IL-15 in the lumbar spine, femoral neck and other areas (Ward triangle, electron, femoral, total femoral) using age, years since menopause (YSM), weight and height as covariates The relevance of SNPs was analyzed using multiple regression analysis.

As a result, four SNPs (+ 20A> G, + 13467C> A, + 13653A> T and + 13815A> T) and two haplotypes of the IL-15 gene were significantly associated with BMD levels, as shown in Table 5. It was. In the co-dominant model, the SNPs were significantly associated with low BMD of the lumbar spine (p = 0.01-0.02) and femoral neck (p = 0.002-0.006). In addition, the SNPs were significantly associated with the lumbar region (p = 0.006-0.03) and the femoral neck (p = 0.001-0.009) in the model. The effects of + 13467C> A and + 13653A> T on BMD were dependent on the amount of genes in the lumbar region. Highest BMD values were found in homozygotes for co-alleles of + 13467C> A and + 13653A> T (0.895 ± 0.179 and 0.9 ± 0.179 mg / cm ² ), with intermediate BMD values (0.881 ± 0.167 and 0.874 ± 0.163). Mg / cm ² ) was found in heterozygotes for a few alleles, and the lowest BMD values (0.878 ± 0.153 and 0.873 ± 0.148 mg / cm ² ) were found in homozygotes for a few alleles. Although the BMD in homozygotes to + 13467C> A minor alleles is slightly lower than the heterozygotes, gene-dosage effects have been found in the femoral neck. The effects of + 20A> G and + 13815A> T were found to be the highest BMD levels found in homozygotes for a few alleles for co-alleles in the lumbar and femoral neck, intermediate BMD levels in heterozygotes, and homozygotes. The lowest BMD levels and gene mass dependence were reversed.

In addition, haplotype analysis showed that ht1 (GCAT: + 20G, + 13467C, + 13653A, and + 13815T) and ht2 (AATA: + 20A, + 13467A, + 13653T, and + 13815A) showed lumbar spine in the co-dominant and dominant models. = 0.01 and 0.02-0.005) and femoral neck (p = 0.002-0.005 and 0.002-0.001). In addition, the genetic effect of + 20A> G on low BMD levels was detected in the former (p = 0.02), femoral (p = 0.04) and total femur (p = 0.02-0.04); + 13815A> T in the former (p = 0.02), femur (p = 0.03) and total femur (p = 0.03-0.04); Ht1 in the former (p = 0.01-0.04), femur (p = 0.03), and overall femur (p = 0.01); Ht2 (Table 5) in the Ward triangle (p = 0.05) and the former (p = 0.03).

That is, BMD levels decreased in minor alleles of two SNPs (+ 13467A and + 13653T) and decreased in co-alleles of other SNPs (+ 20A and + 13815A). In addition, ht-2 was composed of BMD-reducing alleles, and ht1 was composed of BMD-increasing alleles. The results suggest that four SNPs (+ 20A> G, + 13467C> A, + 13653A> T and + 13815A> T) and two haplotypes (ht1 and ht2) are genetic factors associated with BMD. Therefore, minor alleles of + 13467C> A, + 13653A> T and ht2 are associated with risk factors for osteoporosis, and minor alleles of + 20A> G, + 13815A> T and ht1 are associated with protective factors of osteoporosis have.

<4-2> Correlation with spinal fractures and non-vertebral fractures

Using the age, YSM, weight and height as covariates, the association between IL-15 SNPs and the risk of fractures in the spine and non-vertebrae was analyzed using a logistic regression model. Akaike information criterion (AIC) for all possible models was statistically predicted for proper model selection using stepwise model selection (SAS) (Koh et al, J Hum Genet 51: 112). -117, 2006).

As a result, spinal and non-vertebral fractures were indicated in 115 and 96 patients. Polymorphism and haplotype were not associated with an increased risk of fracture (no data).

1 is an IL-15 gene map.

2 shows a haplotype of the IL-15 gene.

3 is a diagram showing the relational imbalance constant │D′│ and the LD-dense region between four SNPs (C / C: homozygotes of co-alleles, C / R: heterozygotes of minor alleles, and R / R minor alleles) Homozygotes of genes, Pa: P value of co-dominant model, Pb: P value of dominant model, Pc: P value of recessive model).

<110> KYUNGPOOK NATIONAL UNIVERSITY HOSPITAL <120> A polymorphic marker of prediction of osteoporosis and the          prediction method of osteoporosis using the sam <130> 7P-11-25 <160> 15 <170> KopatentIn 1.71 <210> 1 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> -156T> C forward priemr <400> 1 tgctactaac cttcctccat acc 23 <210> 2 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> -156T> C reverse priemr <400> 2 gaacaatcca aatgtgaaca gac 23 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> + 13467C> A forward primer <400> 3 ctccctgttc tctttcctcg 20 <210> 4 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> + 13467C> A reverse primer <400> 4 tttgactatc acccttaaac aaatg 25 <210> 5 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T forward primer <400> 5 cagaccttgg atcagatgaa ctc 23 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T reverse primer <400> 6 caaagttgtc ctgggccg 18 <210> 7 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> -156T> C forward primer <400> 7 gatgcatctg ttaagtaact aatagagtgc ttat 34 <210> 8 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> -156T> C reverse primer <400> 8 tccattaaaa acacaagtgc actagtaaga 30 <210> 9 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> -156T> C probe-1 (FAM) <400> 9 agtattaatt cccttccttt ct 22 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> -156T> C probe-2 (VIC) <400> 10 tattaattcc ctccctttct 20 <210> 11 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T forward primer <400> 11 tgaacttctc tcagacttac tttactcatt tttttaat 38 <210> 12 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T reverse primer <400> 12 tcagtgcaac ttaaaaaaca acacttgt 28 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T probe-1 (FAM) <400> 13 tggaataatg taaaatgttg 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> + 13653A> T probe-2 (VIC) <400> 14 tggaataatg taatatgttg 20 <210> 15 <211> 98819 <212> DNA <213> Homo sapiens interleukin-15 <400> 15 agttaatctc cacttctttc taccctgtaa ttttattagt tatcttaaga cagtttcaaa 60 ttatttcatc aatcttcatc tttgctttta atttatgcag cagaatttac ttgttcattg 120 gtatgagtaa ttttcaatat tttgccaaac agcctgtggc aactgaaaac acaagttgca 180 tacattagac ttttcttttt gagtgcaatg caacaaaaac aaattatgtt gcaaacccaa 240 cttcattgaa ctgcaggcac tcataactaa ttaaaaatac ttttaaaaca tctgtattta 300 tgaaaacaaa tttgaccaac atatttggga ggcaaatagt agagcaaagc atatcttatg 360 actataataa tttttagttg tactttactg ttatttatgt tttttatctc cctactctga 420 caaaatgctt tatatatcat ttagtccatc ttgttctaga acctactata gttgaaaccc 480 aataaacagt tgtagaccaa ttttcttact gttatatact cagattatgc cttaagttca 540 ccctacaagt aaactttccg tcaatttcat agtgttgtga aaactctcta gtagctcaga 600 acgttcttgg tggtctcaat atattaaaag tatgcagtca gatactgatg gccctgagtc 660 ctgcagacag ctaacaaagc tgaagcccac aggctagtga gcaccagctg agagcacttt 720 ctatagagtt tgtgggcagt ccaagagaga caggatctga gatgtgatga tatgtcctct 780 ggcagcagca atatctgata ttgagctctg ctaggtggtg ctgagcgtgg acacctgctt 840 ccttcaccgg ctgcagagaa cacagtatcc attaaaggag ctgaaagagg tgctagagga 900 gtttacatgg caggagtcca ggaagctctg caacagtcca aaatgaggtt gggacacatg 960 atacacttgt tcatccctat tctctatctt cacacatatg catatcagtc cttccgtggc 1020 tgctatattc ttgtggtgag atccaaacca gctgcagtct tgctctccca gtgcatgctc 1080 attctgcaaa tcagagcttc aatgtattgc tatgaaaaat tctgactggt cttgaaatcc 1140 attgacacca tcacaagaaa attcaacatg acatcgtcat gaacagaaat ggatgatggc 1200 ttcatattct taaatataat ttccaactat ggtgaaattt tgaatggttg taaaaactgt 1260 ttttgcattt ttatacctca cttattttgt taatggagac tatctagagt ttgatgtaat 1320 tctcatcaaa aagtccatca gtaattattt caaatataaa actgatatca aaaaattgag 1380 ttaatgaaaa taaactcaaa tttttcataa aaatatgaaa ttatattata cggaacttta 1440 tgtacatgga aagtgaataa tttctattat ttttctattt gatatttatc atatttatgt 1500 aattaaaaac ataattaaat tttaaactaa attaaactgc gctccaggaa gacatcattt 1560 tctctgaacc atgactctga aaatttggaa agcaccgtac aagagggatt ttcagtgatt 1620 cacaagagtg caagattctg caaagtactt tcaaaactaa gcgtgagatc tttgctactg 1680 cctttatggc atgcaaatca ctcagcctca actcagttcc ttcctccaat gtaaaaatac 1740 tacaagaaca acatctcacg cttccatcaa atgggaccat gtgtgatatt ttacactgga 1800 ctacacaata ggctggaaaa gtgatccgca gagaggctca ttgctctaag ttcactccct 1860 gtaccaactg tcttgcacaa agctgccacc cgggtgtctt cttccaacat ttcccctagt 1920 tggacttcaa agtttccttc ttgcagcctg tgcggggacc aagaggccgg tctagagtag 1980 ccgcccagca tccagatccg acatcctttt ccctttcctc aggaagactt ggatgcgcgc 2040 cagcccagcc cgggtctcca ggttcagctg cggaagcttc ccacatggtg cccgtcgctt 2100 gggatgcggc tatccttgtg ccttctctgc aggtgtcccg gcaggtagag gaggagaccg 2160 gtggctggtg ttcctgcagg gcggggctgg ggctcctcga tgtccttgcg cttgccctgc 2220 aagttctgcg ccccaggccc cggcagggac ggaaacaggc cgggaagcag tgacacctgg 2280 ggcagtcagt catccaggag ccgccgccac ccgccccacc cccagtctcc gcgggaggtg 2340 tttgtcttcg cctatgctcc cggctctttc tctttcactt ttcttttcgc caggggttgg 2400 gactccgggt ggcaggcgcc cgggggaatc ccagctgact cgctcactgc cttcgaagtc 2460 cggcgccccc cgggagggaa ctgggtggcc gcaccctccc ggctgcggtg gctgtcgccc 2520 cccaccctgc agccaggact cgatggaggt acagagctcg gcttctttgc cttgggaggg 2580 gagtggtggt ggttgaaagg gcgatggaat tttccccgaa agcctacgcc cagggcccct 2640 cccagctcca gcgttaccct ccggtctatc ctactggccg agctgccccg ccttctcatg 2700 gggaaaactt agccgcaact tcaatttttg gtttttcctt taatgacact tctgaggctc 2760 tcctagccat cctcccgctt ccggaggagc gcagatcgca ggtccctttg cccctggcgt 2820 gcgactccct actgcgctgc gctcttacgg cgttccaggc tgctggctag cgcaaggcgg 2880 gccgggcacc ccgcgctccg ctgggagggt gagggacgcg cgtctggcgg ccccagccaa 2940 gctgcgggtt tctgagaaga cgctgtcccg cagccctgag ggctgagttc tgcacccagt 3000 caagctcagg aaggccaaga aaaggtaccg cagctcccca cttcctaact gctcccgtcc 3060 caaactcatt gtgggccggt gcagatgtct gtgcgtgcgc gtctatccct acctttgcaa 3120 aatcaaatta tcgccctctt ctttgcttgc acacgtcttc tgggagagaa atttgcagga 3180 atggtcagtg cttggcagtc aggcctgggt ctgtattggt tccaactctg cgccagccag 3240 ttatctctta gagaagcgat agtgtatcta gaatgtcgaa ggaacttgga aaaaatacga 3300 gcgaatttca ctgaaataaa atcattattc aaggtggctt ttaaatgaaa atccagccaa 3360 ttttttcaag tgttattaag accgcatgta gcaagggtat atagttcatt tgactttatg 3420 taattccatg cattaaaaac tatgttgact tcaaagtatg tctaaaccaa actaattctt 3480 aaatgatgct tacaacagat tcttaaatag gatgagtcag caatttcaaa actggaagta 3540 aagtggtggt caccagctag tttccattat atatttttaa actactatct aatattatga 3600 tactggtggt ggccaactaa gtggacgtca tccttatctt gtcagtgtgg tgaaagaggc 3660 catattagct aggctgaatt tttgctcagg tattacagaa taaactggtg attatgcatc 3720 ctgccatgta gaggttataa ggtgaaccat tctactgaaa gcatcaagat acaatacact 3780 tttggaaggc cgaggcgaga ggatagcttg agaccaggag ttccagttta tagtgagcta 3840 tatgatctgc cactgcactc cagcctgtca aaaaataaat aaatgaataa aaaagatata 3900 atacaggtta aatttttaaa ctcctgttgt tttcgtgtga agtaatgtgg ccaacagagc 3960 aaggaaatac atgggaagga acataaagac atataaagag aagaactgaa aatacagcaa 4020 aagccattta gatttaaaaa gtgaagaaaa agctagtttt gcttaaagtt aacaagtatc 4080 atagagatct taaggtttgt tatgattgtc ttagtttcgg gtccttattc gtgtaaaatt 4140 ctcacagaat tttagtcaat cagaaaagtc tctcctgcag tatcatctgc tttgcaacag 4200 tgacagagaa gggtcagttg accattggtc tggaacttct gataggcagg gtgttcccat 4260 acttttggtt tcactgttcc ttgctgattt ggagtggcaa ctccagaacc tgattggaag 4320 ctatagttct ggttggaatt ttcctgcaga ctactggtga ttaaaatgat gctcttttgt 4380 tttaatccat gaaattaatg tagccaatgt caacagctat attgcaaggc acaatgttta 4440 taaatttcct tgggggatgg agaaaacagt ggtttaaggt ttatcgaaat agattttgga 4500 agtggaaggt tttaaagcct acgggatact ccatctcaga tttgctgcct ctgatgaatt 4560 tcaggacagc tagaagagca gccctgaaat gctgggtaca tgtggatttt tctcaggaaa 4620 atatccgtaa ctttgatcag ctttttaaat gggtctctga cccaaaagag ttttaaatct 4680 tttgaaaata tagactactg tccttttccc ctcatctctc ctggagttta atgttgagtt 4740 ctgctcattg tgttaactat ttttctgaag gttagctgat cataatacag agttctgtct 4800 tcagacctat aacttaagtt atgagaaaca gtatgaataa taactaacat tgttgagttc 4860 ttattatgta tcagacattg ttctaaggat gtatttttta aaaacatcgt aataaactta 4920 tgtggtaggc aatatatttt acagctgagg aaattatcaa ggtcacagag ctagaaagtg 4980 gtagagttgg aattccaact gagacaagtt taagactggt ggtatttatg gaggatggta 5040 ttatttgcat acaaatattt cgtacaattt gacagtgata atccctactt attttcaggc 5100 agcgttgtaa tggtagaatg ccggttttaa agtaatgttt gtatggtact tgtgcaattg 5160 acattattat atatttaaag attatcttta taatcaaata atattcattt gagtgtcaaa 5220 tatgtgtttg gtatattgga gaaatttcct ttatggtccc tgaatgaaaa catttgtcta 5280 atctgcatca ctttctgctg aaagagcctg acaaatagta ggcaaatgtg gattgatcta 5340 gatatctgtc tctgcctatc tatgtatccc ttcttccata caactagaag tctgggattc 5400 atggagaggt cagagaagaa gagtttccca taatatccca actggagatg atatagttaa 5460 ctatttggga catcctctct tcagaaggca ttatggtaga aggtttggca agaagccact 5520 tcttttgctc gatattagct ctttgattta cttacccgtt gattacagta actgcatgag 5580 actactgaca ctgaacattg cttaaatact tgctgctgtc ctataggaat gggttggatg 5640 gaaaaattcc atagaggagt tgaaggaaaa attgttattt tagtgagtac tggaatgcac 5700 taacacctat ttgatggtaa atttcactaa ttaaatacta tcaactatat gtctatatgt 5760 gtgcatatat gactatatgt gtatatatac tgtgtatgtg catatatgtg tgtgtatgta 5820 tatattgtac tgtactattt gttaacagct gtgggcagac aaggtttact ttttactcag 5880 cttccagact gtattgaata tataaacata agaagctaag aaatcttgac agaaagcctg 5940 aaggacaaat tattgttatt aacagtgcca ttccagtagg aacctaatgt gaatttgaaa 6000 gtttcaaaga ggaagcctct gctatgtttc agccaccata ctggcacttc atctgtatgt 6060 cctcattgaa tttgaataac cattgcattt ttatcctgaa tttacaaaga aaaaaaaaaa 6120 aagacatgca gagttttgct tgcattagga cacaactagg taagacagac actccactga 6180 gatttatctt gattctaatg ttaatgctcc ttttcggata tcatatgcat tctcttctaa 6240 gaaaactcag gtgttcagtg tactgacaac taaaatgctg ctatccttac cttgaaatag 6300 accctggtca ccatttgggg tgatttaatg ctatttatga atttgcctat gtcaaagaat 6360 attgcccttt tttaaacaaa caaaaaaaaa agttgtttag tgctggagtc agaaagagga 6420 gaaaaagagg ctctttaatc tcagtttagt tcaataatat tatttgtcca catttcacat 6480 ccagagccag ggtatttgta tgcatttatt ttatagtgtt tcatcccact gcaggggcag 6540 aagactctat gtagaccgta taaaaatgga tttttaaaaa gtctgcatgt aatacacagt 6600 tgtttcttct ctaaatgcta atagttcctc caagtgctga cagggtgcag acaaaatcag 6660 gaaagtgaca aattattttg tgaaatgagg gaattgaaac tgaaaatggt tgtagaacag 6720 gaaaatatga tggttgtaag gttgatggcg gtggtggtgg gggtaagctc tgcttatcat 6780 tgtgacacac gaaaggagcc cagagagggt ctttggtccc ttatatcact gaatgctact 6840 tctccttcct gggtattttg cttatgtgcc ttctaataga aatctgacaa agtagtcctt 6900 gcacactgtt agcactgacc tctaaaatta tcaaatgatt ttttcttaat tgaacatttt 6960 actgttaaag attttctatt ttcctgactc atctgcaaca aaaataagta cataaaactt 7020 gattttttat ttgaaggatt ttcgttgtga aaattattct agcttatgtt atttaaaaca 7080 ctatcattat aaaattaatc aaaggaacca ttaatttttg ataagtgatt attaagcata 7140 aaactaaaac atatataccc aaaggattat aaatcatgct gctgtaaaga cacatgttta 7200 ttgcagcact attcacaata gcaaagactt ggaaccaacc caaatgtcca tcaatgttag 7260 actgaattaa gaaaatgtgg cacatataca ccatagaata ctatgcagcc ataaaaaagg 7320 atgagttcat gtcctttgta gggacgtgga tgaagctgga aaccatcatt ctcagcaaac 7380 tattgcaagg acaaaaaacc aaacaccgca tgttctcact cataggtggg aattgaacaa 7440 tgagaacacc tggacacagg aaggggaaca tcacacaccg gggcctgtca tggggtgggg 7500 ggagggggga gggatagcat taggagatat acctaatgta aatgacgagt taatgggtgc 7560 agcacaccaa catggcgcat gtatacatat gtaacaaacc tgcacgttgt gcacatgtac 7620 cctagaactt aaagtataat ttaaaaaaaa aaaagccgca atgtggtttt cgagcttcta 7680 gaaacttaga aaggtgctaa gtgctaagga aatttcttgc atcagtgctt tgaatgtaaa 7740 ttatttttgt tgaaaagtgt gaatatcgct acgttttgtg gagttttgaa aacaaaacct 7800 gtattggaga ataataataa taataataaa taataacgtg ctcttgaaaa aaaaactaaa 7860 acaatattgg aaatatatat catacaacta gatatgtcaa tgctttgatt aaagatgtct 7920 cttatatcaa cttttcaaat tgccccttta tttggcaccc tggtgaggca aggcacatgt 7980 taagattctg gatagggaga gataggcatt tttttcctaa agtgggagaa gggtaattat 8040 gaaagaggaa gtgaggaagg agaacttgcc agaaatctgc ctgctttggg cacacatcag 8100 gcatatctct ggtaaaaggg gctcctatgg aagtaacgga tcttgaaatt ggttccttta 8160 gaaccgtcta tgtccacaaa gccctgggaa agttgtaggg tatgccagag gtgcgtgttg 8220 ttcagttggg atatatagtt cagccttctt tcccacaccc tccctttggc ttctggatta 8280 ccatgggtta ctgtgcttaa agcaaagtac agggagtagg atggggagca gtaaagagcc 8340 tgggctagct tctgtagagg tttcccactg gggactagtg cgaaataagc tctagtaggg 8400 cttggatagc ctctgcagtg cagtctcctc ttctagcctc agctgcagtc accttgcctc 8460 agtgtccaga cacacttgcc attctttgtc agccttcatg ggtcctgctc aaaatgttaa 8520 ccctctataa ctttttctgt ccccacctac tgcccaggca gaattatttt ctcccttaca 8580 tgaattcatt tggtgctgtc tacattccct tataacagct ttaatgtctg taaactcatt 8640 tttatgtgtc tgactctaca cttacatttt gagtaagggc aatgtgctcc atgtgcttag 8700 cagagggttg gtcagagtct atttgtattg acttcagcat gagtcaatca tctttcaagt 8760 gtttgacagc taagcaaaca actgtaaaag aggtaggaaa cataagtaat atagcaaaac 8820 aatgttggaa tactgtaata aaaatagtat tttagggaaa ttagtttgag gtagaattta 8880 ttgaagaaaa gacaattaga gataaggaaa ccagctagaa attcatcaca gttaattcag 8940 gagtgtagtt tcattctcat tatctgctca aggacataac tctagtaatt cattctttct 9000 ctccatcacc atttttttct ctgtctctta tgggtctttc ccaataccat gtagccatgc 9060 tataacttct gccattttta aaaaagactc atgtttttct cctcatgttt ctgctcactt 9120 ctatatctgc ttttttctgc ccacatttta caggaaaact tacaacctta actatattct 9180 ctgtttccag ttttcctctc tcctttctct cctgaaccag ccaggctgtc atctccactg 9240 ctccactaga aatacacttg tcaaaggtac cacgaacatc ccttttatta aatccagtag 9300 ttagttattg ggtcccttcc tatgtaccct ttcagcagga cctgagagtt cttcatgtgg 9360 cttccaagcc actccccttc tccttatttt ccttttacca taccagcaac aatttcagtc 9420 tccttttacc ttaccagcaa caatttcagt ctctgtctag ttcctaattt ccctaacatg 9480 taaatatagc tgtctgaggg ctcagtcctt aggcctgttc tctatctgta ctctctgtct 9540 tggtgatctc atctagtgtc atggttttta atcccttctt acattgatga ttcacaaatg 9600 tacgggtgaa tctagccatg acctctcccc tgaactccag actcatttag cagtctattt 9660 agctgttatt caaccatctc tgattggatg tctaatactc actggattcc tgcctttact 9720 gccatatctg cttgctccac cttcttcctc atcttacctg atgtcaattt cattctctta 9780 gttccataga taatcaatca atctatcttg ggtttgtcct taacgtctct ttatgtcata 9840 ttccacgttg aatctgccac caaattattt agctaggcct tcaaaatata ccctacatcc 9900 tatctcttct cacccttttt ttcagtgctg tcacctgggt tcaagttgct atcatgtctc 9960 acagtattat tgcaaaagtg cctgctgctt gtccccttat ctccactcca gcctgttctc 10020 aacatagcag ccacagtgtt ttggttacct gtccctgcat ctaaaggatc aacagccata 10080 aaacaagaac aaaacaaaac aacaccccca gaacttagtg acttgaataa caataatttg 10140 ttatttatcg tgatcttgta atctgggcta ctttcggctg cttgactcct cctaggctgc 10200 tggagatgag acattcaaga aagcatcttc cttcacatgt ctagaacatc agcttggata 10260 gctggaaagc tcatggctgg ccaagcatcc atctctccgt atgtgacccc tatacatgac 10320 taacttggac tttacataat gttggcctca agtagttgta tcatttaact aattattgac 10380 tttcaggatc ccccatagct tatctcgtta tggcatcaaa ctcagacttt aagtctctaa 10440 actggatctc cagatgagga tgatatttct ctgatgtagt ttcttgagtg cagcaacctg 10500 tagctaaaaa gataggttat ctgtgacctt ccccttgtcc caacatacaa tggtaaaaca 10560 aaaataggac aattgcttta aaaaaatacc atttcaaaac aagacaatag aaggcatata 10620 acagttgcta gtccatagca agtctaaata gagccggcat atatcccaag ttcttgctct 10680 ggaagcaata catgttccgg attaaggttt aaatttgttc cctaggagtg atcctctgtg 10740 gctcttaact tcatcttttg cactttgggc tccttttctc attctttctt tcttttctat 10800 gagaaataat ttttgctatt ctttgcagtg gcgtagcctg ctttctgatg ttagagggtt 10860 aataatttag agacctttta tatactcact tttgtttaac tgctttttgt ttttaagttc 10920 aatgatattt agaattcggg gggcttccta tgaattttat tgagattcat tcactccgtt 10980 agataaaaga cataaccaga aaatccttga gacaagttcc actttaattt tttgggaagt 11040 caaggtgcca tagaaaccct taccattttt agaagccttt tgactagttg gcaaggtctg 11100 tgagacatca ttttaaatct ttcagagttc ttaatgaagg gcctccatgc cacacacctg 11160 atttgatctc caacctgaga gcacatgtaa tagtagagcc tggatttgat ctttgccctg 11220 aaactactta tttcttggag aatcttttgc tgcctggaaa ggcaagaatg agaataggtg 11280 ttattttcca atctagcaag tcttaggttg aactatttcg tcactgcatt tctagccttc 11340 actaacaggg tccttgcctc tctccaggtg tcttcccacc actcagtctc atagtagttt 11400 caatgtcaat tactaatttc catataatag actaccccaa cacttagtgg tataaaataa 11460 gaaccatttt attttctcaa gcctaagaat taggacaaaa cacaaagaga atgacttgtc 11520 tctacactgc aatgtatgtg gcctcagatg gtatgactca atggtcaagg gatgtctgag 11580 atggctcaga cattaagtca cacatataga gcctcagttc tggctgttgt ctgggctcct 11640 tggttctcct ccacttagtc tttccacatg gctaaactgg actttctcac agcacggtag 11700 tttcagggtt gtcagactct tcacaacttg gccaacttct ataagagagg aagttggagc 11760 tgtaatcact taaggcttgg atatggaatt tccagacttt aacatttgtt ttattctttt 11820 gggaaaagca agtcagaagg ccacccagat tgaaggggag acgacataga ttccaccttt 11880 tgattagtag agtgtcatgc atgttcatgg ggggaaggca ctgactgatg gcagctgtct 11940 ttggagatta tccaccacaa agggtaatcc ggttaaaata ttaatcaggt tatgctactc 12000 ctctattcca aaccccacat ggttctccca tctcactcta ataaaagcca aattctgtta 12060 cagtggatat taagtctctt aagtgatttg tacccctgat gtctgccatc tgctactctt 12120 cccttagctc actgagtcag ttacactggc accttttcta ttcaccaacc atactactat 12180 gttcttgtct aagagtcttt taccttgctg ttccttcttc ctagaatgct tttctctcag 12240 atatcacccc cttccccata cctgctttgg ggtttgtctc aaatatccct gttgcaatga 12300 agtaatcctt gactgatgta cttcaaattg cagcctcacc agcactccct gtcccaaccc 12360 tgtttgggaa ttcagattat ttttcagatt ttggaaagta atatagtgtg tttactatat 12420 atcatgtaac gaccaagctt attcaggtac ttcctcaaaa gggaataaat acagcttaca 12480 catagtctaa tgttagttca gcttgtgttt tgttaatcag tgaattaagg agaaactttc 12540 agtttttgga gcttttttga tttaatattt tcatataagg gactatggac ttgtatgttt 12600 tattgaatta tttgattatt aacatctccc ttcattagaa tatgaaccac aggagaacat 12660 gcagttttgt tattttattc attgctatat tcccatcacc tattagtgct aggcatatgg 12720 tagtctctca acaaatattt gcttgagtat ttaaagcaat atttaaaaca aaaatgaatg 12780 agggaatgaa tggattaatg aatgctaagt agtagggtac tgcaaataga atggagaata 12840 gaaagacaag ctaatttcaa ggaagatttg atataccagt gttctattta ttcattgaat 12900 tgattttggc acttcagcag ttaataattt tgagtgccta atatgggctg gggactttcc 12960 taaatacttg actggtcttg attgagtggg aggagcagag cacagcgaag agtcagagaa 13020 tgccgaagat tttaaccatg agagtcagga aagataaaca tgttaggaac agatatatat 13080 tacagctaac acatggggca aggtagggga atggatgaac ttgattttgg aaatatcacg 13140 attgagttaa aagtggaata aactacttaa aatgtcctgt aagtgattag aagtactgga 13200 ttgaagattg gataagctat ttgtacaaga tttgggtttg tgaattaagt aaagaattat 13260 tggttaaaac aaatggtcag gatatttccc caggaggcaa atgtatatat agatggctta 13320 gggtcacata agacaactag agggactgtc actgagctaa gcaccctgaa agaggagata 13380 ttcgggaagg ccttcgagga ggaattgacc agctcccagg tcttcaaaag agaaggctag 13440 gctttttaca cctcctagac cttgccttcc tgtcatactt gctctgtctg ctgctctcta 13500 gagggatttt aatccctcat atgcttattc ttacataggg atcatcttta aagactcact 13560 ttctctgtga cgttttcctg gcgagacact atctttacct cctgtcttgc ctgacacctt 13620 tcctttgtgt gttctccacc tgatagatac ttctctcata gtatctgcag tgtttcactg 13680 tatttatctg tgttcatatt gcctctccgg caggtaaatg ataaaggtca tagatgagaa 13740 gaattgtgat ggtggagttg gaagggctga agtttgagaa acactgcatt cttatgtgtc 13800 taacaccttg tctggcacat agtagagcta taataatgtt attcttgttg tcaatgatcc 13860 aacatatatt cctgaggggt caagttagat caggttctga gaaaaggagt ttgaccatat 13920 ttgactcata gtatattact ggtgatcaca tggcttttgg gaaactattc acagcatatt 13980 tcctattatc agggtcagga agggaaatag aattttctgg ggatgtactt tgggccaggt 14040 tacaaataga atctttaact tccttattag gagtatgagt tttagagaac tgaaaacaaa 14100 tacgtttgtt taatattttg tgccagtttt attatagagg ggataatttt ataaatttgc 14160 aaagtaaatg cttcagtaaa tgaaaggaaa ttaaaacaag gcacatctta ttctctaaat 14220 tccttcaaac agaaatcctt tcattttctt tattttattt gccgagtatt ttgagaagtt 14280 atttcagtag gacaaaaaag atgagagaaa ttgtgtgaaa ataatagtat tgcggaagtt 14340 taatttattt agtgtctctt ctcctgaggg tttaagagaa gaaatatggt ttcttctctt 14400 gttatgctgt taaaaaaatt tgcagttacc tcaaatatca gattaaaatc tgtatttggt 14460 atctttagat tctactcctg aaaagcaaga tccttgtaaa gaacctctaa aactggaaag 14520 atgtgattat gctgatggct tatttgactt gatattgagg cattcatctt ctaaaccttg 14580 gcctttctta agtcaaacca tattactctg agccgctgca acttataaag catggcttcc 14640 aggctaaggt gtttaaacca aagaggctaa cagcacacca aaagttctaa acaatagtgg 14700 atatttattg atcactttct ctgtacatgg ctccctgcca agtgctgtga aggataaaaa 14760 gacgcagcac atttgattct ctttaggaac ttattgtctc gtggaacaat tattgtttgc 14820 atgtaaacag ctgagtgtcc aggatactga actactctct gcccctaaac acaacacact 14880 tcctttcata gccaaatatt ttgacatgtt tctctcattg gaatgcacta cttcatccag 14940 tatctcttcc gtgccccctt ctcctgactc gcctggtcca gaaatctcag tctgataatg 15000 ccttctctgg gaatattgtc ctaaatgtcc aaagttgggt taggtgtctt tcattcttaa 15060 cttcattcca taaatatttc aatagttaac atttattttc acccatagtc ccaagtacgt 15120 taacatatgt agcattctca aactcatgag atactttcct tcccactttt cagataagta 15180 aactgaagca ccaggaagtg tggtgactgg tgcagggact accagtagga agtggtggaa 15240 ctggagcttg aactcaggtt tctctctcaa gcatgcacac ttcctataga gggtgctacc 15300 ttgctggtgc aattaataag aattataaag attaaaagag aggaggtgaa attttcatta 15360 tttatagatg aaagccatac aaattaacaa gctactaaaa caaatgggag agctcagaaa 15420 agttgccaga ccaaagattt ttaaaaatca ataacatttt tcaatagtaa ataaagcatt 15 480 agctaacata acacaacagt aaaacaaacc agcaacacaa ccttccccag taggagcacg 15540 cttgctgcaa ttaaatatgg tttatttgag gattgtctgg atggctcaac atcagaaaat 15600 ctatcaaaat aatcttaagg atataggaaa agttagtgtc aagttctgag aagaataaaa 15660 atagattgga ttttccaaaa ctgactaaag tatatctatg aaaaatctat agtaaacaac 15720 aaatttaata cagggctcag aaaaacattt tttgttttac attttgggag acgatgagga 15780 tgccagtgtt ggcacaacta ttcagtaaag tgatgaagac aaaaataata atcaccattc 15840 atttgtattg agtgtttacc tatgtgccaa gcagtataaa agcaaaagtg gtggattagc 15900 cattggtgca aggagatggt gaaactccca tcttcctact cctccaaggt agaggtgata 15960 attgaactac agtgagccag gatgaaatgg agttgtcaca gaaaattgta actaggatgc 16020 ttaatttatt gttctgaccc agaatgtagc tatttggaca taatggaatg gagcaaatta 16080 caatatagta gaatttgggt ggtaccaatg atggtgtagg atagttagat ctaagctgtg 16140 agatgaaaaa atccctcaat gtctactagt aactgcgctt tagatatagt caatacatat 16200 ggaggtgcaa gccccaaaca tactgttatc atggatttta tggatgaagc agagcccttt 16260 tgaaattgaa gttaatatgg agaagcagca caatgcatag catatgcttt gtgctatggt 16320 aactgaatga acttctggtg aagaattaaa ggagggatcc aaaacacatt gctaaatttc 16380 aatttaacct ctttatgtaa aattgaacaa tacatttaat ttctctgcac ttattattct 16440 cttcttgaaa attaagataa ttagaccaga gaaaatccct ttggtgttga atctggtggt 16500 tcaggaaacc aacataacat ggatggattc tatcatatgc tgagattttc aacctgtcat 16560 ctttatagat gttacttagg cactggaagg gactttagac acctccagtc taatctccgt 16620 attttaaaga caaggtaact gacacttaaa gacataaagt accttgctag tgtcacatag 16680 ctgtttaaag gcagaatcaa gcctagggtc caagtctcta gaccccagtc cattccatgg 16740 ttctggtaag attaggaaag ctccacattt catcaagaag ttctcaatct caattttaca 16800 tgaactatag ttttggaaat tgtaatatgt taatgatgaa tagatgggaa tgatggtagt 16860 ctgtatggag tataataaaa gaatagatga acacttccac cattatttac acaacacatc 16920 caaagagatt ccacagtgtg cgtgtgtgta tatatacaca ccatatatat atatacacac 16980 acaccatcta tatatataca tatatataaa cacatatata tacacataca caatgaaata 17040 ctattcagcc ataaaaatat taaaatcacg tcttttgcag caacatgacg gaattggagg 17100 ccattatctc aagtgaaaca aatcaaaaca gaaagtcaaa tgtggtatat tgtcacttag 17160 aagtgggggc taagtgatgt gtacacatgg acatagaatg tggaataata gtcattggag 17220 aatccaaaag gtttggagga tggaaagagg ttgagggatg agagattact taattgtaca 17280 atgtacatta ttctggtgat ggttacacta atagcccaga ctttacctct atgcagtata 17340 gccatgtaac aaaactgcct ttgtatgcct tacattttat ttttttaaat gaatgttgtg 17400 gtcccaccac atggaccaca tttaatgtta gtttcctaga agtatgtttt ataactgaat 17460 agagataatt ttctatattg tattccaacc cggatgatat acatatatat tcggtaagat 17520 ttttcaattt tctttgttct cactccgtat ttatcaaaaa ttaattttat gaataatttg 17580 gtcatgtaag ttaataatgc aggcttagtt actcagggat tgctctggag caagaaaaaa 17640 gttactcact tctaccttct gtaccccttc atctttcact ctgccactgc caataaaacc 17700 ctccaaagaa atccgacatc ttagactaca aaaaatgcag ccatcttggg aaacctgtag 17760 caagtacacc cagcagaaag ggtcaaaatc ttaaagattg tgacaaatct gtaatcccac 17820 agaagggatg aggcatgcca agtgtttgat gtctacaaaa accattttcc ttagacaaaa 17880 gctgaaaggc ataggagaag taatataaag cagaaaaaat gaaaagcaaa aattataaag 17940 aagacaagaa tggcaatcta tttataataa tagttttgat aacaaccatt tagataagaa 18000 tagttaatga ggccagtgaa agaggagata aagtactaaa acggactatt ctcctattgt 18060 gaggacagga tctctgttag ctctgtctca gataatttgt acagcccaag tggagaagga 18120 agaaccagca gtgctataaa agataagttc tgctgtccaa tctagggaga aaaaagcaac 18180 cacattgacc acattagagc tcaggagtcc aaacactgct agttgccaaa taaactttat 18240 taatagagac gacctccccc ttagcagttt tataagcact attgcttttt tatatagact 18300 tgagaaggtt agtttacttg tcagaaagta ggccagaaag caatgtttta gaaacaagac 18360 gtttgggact gctacagaga tccaatgctc aggctaaatc acgtgataca tttcatgtga 18420 agctaagtat ttggaacaag agatgccaga ggtctaatca gcaagaataa taaacaatta 18480 gaagaatggc agccaagcct agagaaactg tctggagaaa gagtactgga ggatttgtgg 18540 tctaatgtgc atttgtgctt agtaaaagat tagctgttcc tagattttta aatttaattt 18600 taagtgttat tcctgaggat tatggtaatc gcatctcttt gcttctataa gggattggtg 18660 agaagcagta gcaatatttg aatccagtta tttgtgagtg ggagatgtac tatatcatta 18720 ggtgtcattt tcaccgaata cgttattatc agcaggtaat aagaaatgtg catcattaca 18780 aattctcatc tggtattttg tgctcaaaca gacaaggtat gtcttctgtg gcagcactga 18840 gtgatcacat agagactggt gtcaaaaaat ggctcttata aattacagga tacattttaa 18900 tagttaaaca attatctaaa gaggcaccac tggaaaattg tttagtaaca attggctgat 18960 ttttatgatg aaggataatt caattaaaaa aattatttac agcctccttt ggttcagaaa 19020 ctgtgccatg ctttgggcat tctaaatgaa ttttgtcttc caggagttca tcttctaagc 19080 agctttatgg agaatgtcat agttttttcc caaattttct tcatatatga ttagacaaaa 19140 tatttaaatc tgtgctacat aatattttgt ggtattttga ttttaatttt atgagtggat 19200 aatacattac catggtttaa acttcaaaag tttcaaagcg ctttccaatt cccttctata 19260 gatgcaacca gtgctatcat tttctagtgt atgtccttgc aaatgttttg tgcagacaca 19320 caaaaatgca cgtatatatt taaccatccc cctttctgaa aacaaataca cactgtcgag 19380 ttaccttttt ctttttactt taaaaataca ccttggatat atgttcatgc taatattaaa 19440 aaaaacttcc cattcttttc tatggatgta taatattaca ttgcatgcat gtattataat 19500 ttacttaagc agtaccctac tgatggacat ttaggttttt tccattcttt tgctattaca 19560 ggaaatattc acatgagtaa tttcaacact gatattctaa agtgagctag agtgactgca 19620 ttcctgtttc ccagcccata ttccatagaa cacaagtctc atgagatgtt caatatccaa 19680 aagggttcta tagttaaata actttgatta actctgggta tttgttgatg tttatagtgc 19740 atactgtcaa attaaaagaa atcttgcaat aatctgcttc gatgtaatcc aagtgtttcc 19800 tgacctcaca ttttctcaag gaatggaata cactattgtt ttaggtcagg ttcttgggaa 19860 gcagactgag atggaaatat acatgcgaga ggcttactgg ggcatgttct aaggaacaac 19920 acctgatgga agtaagggca gatcaaaaca gggtgggcag agggagaagt tggactgtga 19980 tgcagttgga acagtggcct cactggatcc aacagggagc cctctagagg caggatggcc 20040 ctttggggat gttacagctc tggaagggaa gcaggccaga ttcctggaag ggaagcaggc 20100 cagattcctg gaagggagag ctttcttcca ttgaaagcat ttatcaggag gggctcagct 20160 atgagctgtc agctggaaca atgaatttca ttgttcacta caactctgta tagaatcttc 20220 aaatttttaa aggaaatgct tatcagttcc tagagtgtct tgctggtcag agcaagtcac 20280 ttggtctcca gggactcagt ttcttgctgt atgaaatatg gatgctgagc tgaaagttct 20340 ttagagttta taacattctt tgcatctatt tccatttcag tatgtgctaa ttgaattcca 20400 aggtcagaca taagttttcc ccaagacata cttggccata tagaggccta tgatgtggat 20460 agataagctc taacttttta atatttctgc tgtgcagtta tggaaaaaaa gtgcattgtt 20520 ataatgcttt caagtatgtg ttacttaact tggggcagac tcttggggcg aaggtgcata 20580 agagctgaat tagagctttt tattaaaata taaaaatata tttttaaaat attttggtta 20640 cctcttgttg ggtgtttgct ggagaagcta gcttgctatc ttcctaggaa cattggccca 20700 tattgataaa attgaagaaa tatactggca tagggtgtaa aagagagagg agcataactg 20760 gctttaaagc acattcttag gagctcttat ttattctcct tttagaagct caggaagcaa 20820 acaggtatag acaaacagct atagcaattt ctcagggacc ttccatttgg gaatcagatg 20880 ggtctaaatg cccatcctat gtcatttatg gtacttgtga atttcattct catgctcctg 20940 caggattcat caattgtggt tgttattgat ggaagcttgt ttcctggtag gagtggggaa 21000 agggctcagg gaatgggcct ctcttaagtg tgcctggtag agttaatgaa ggttcataaa 21060 gaggatggaa aacatatcca agaaaaagaa aatgttcttt aagataagga aatgggaagt 21120 gaaatcaaat ttaaggcggt attatacaaa catgatgcag agaaaaaagc aaaaagaaaa 21180 aaaaaaacaa ttctaagatt ggtggctgta tgggtgtaag atcaactttt aaactgaatg 21240 taagtttttc taaaaaacct taatcttttt cttcaacaca aagcttcatg cggattcttg 21300 gatcatactg aaatttgatt aagatgtttc ttaatttatt ttataaaata aaagttatac 21360 tactttactt ttttaaatgg cataataagt tggattaccc ttataaaaaa acatagaagt 21420 tacactgttt ttaggttttc taattaaata atctgttgcc atatttatac ccccgaatca 21480 catatacctt aaaatattat actatattat gatgttgtgt tttactactt cgtatttgag 21540 tggagacttt gccacttcac ttttagaata aacgtttaca acttattttc aacaccttct 21600 tcagacttgg tcaataactt aatatcaaac acacaaatac acttgacatc atgtgtgctt 21660 tgtgatattt tgtgataaag agaaagacaa tttactagag agctatatga agcattatag 21720 gttttaaaaa atgatatatg atttttttct ccaaaatgat gtagctttag tcagcaagga 21780 catgagttaa attatgggga actctgagga ttgtagaaaa gatttcccca agaagttcag 21840 tcttgactta tgcagttttc tccatatgag tcttcctgtt tttttatagc atgatcaggt 21900 atgtaggtaa ccataaatct gaggaaggga ggattactca atacataatt attaatcctc 21960 ttatccgtac ctttgactct tacagaatcc attccaatat atggccatgt ggctctttgg 22020 agcaatgttc catcatgttc catgctgctg acgtcacatg gagcacagaa atcaatgtta 22080 gcagatagcc agcccataca agatcgtgta agtaaagttt taaaagtttt atcatgcaat 22140 acagatactt ttatataata gcataactaa taataaggaa aatgtggcaa aacaggtgaa 22200 gttatatata ctcacagctt ttaaaatgag catcttggtt taaaaaatat accctttgtt 22260 attgcatgct taaagcatat atttgaaggc acagaatatt gcacataatt ttttttctga 22320 aaagagccac ttagtaaata ttataggctt tgagagtcaa atgctcactg tcacaatgag 22380 tcaactctgc cctattacct gaaagaagca ataatcgtgt aggtgtatgg ctatgtccca 22440 ccagaagtat ttacaaaaac aggcggctga tgagggttgg cctctgggtc atagtttgat 22500 agtttgtcaa ttctaggcat ataaaatgac agaattgtgc atatcacttt ctgtatatta 22560 ctgggttaat tttgaaagaa cttgcgtgct taaaattaaa taataagtgt aatcaaacta 22620 tctaattttt gaatgagttt atgtaaggat attagaagat aatgtagatt agatttttta 22680 aatgactgag tcttttaagg agtattttag aaaaatgtaa ggtcaagttc agtctcgata 22740 actaaacaaa atatagtaag gtataacttg atgatggaga tatgttctaa gaaatgcatt 22800 gttacacaat tctgttgtgg gaacatccta ggctgcactg acacaaactg agatggtata 22860 gcctatacat gcctagtcta tatggtatag ccagttggtt ctaagctaca aacctgtaca 22920 gcatgttact gtactgaata ctgtaggcaa ttgtaacaca atgataagta tttgtgtatc 22980 taaacacaga aaagatacag taaaaatata atataaaaga taaaaaatta tatcctgcat 23040 aggcccctta agatggataa attttacaat actggaagct gctctggttg attcaatgag 23100 tgagtggtgg gggaatgtga aagcctagga cattacttca cactactgta aactgtataa 23160 acactgtaca cttaggtcct acaaaattta ttaaaatatt tttcttcttt taataataaa 23220 ttaactttag attattataa ttttttaaag ttaagaaaca aacattttct ttaacttttt 23280 gacttttgta ataacacaac ttaaagcaca aacatattat acagctgtac aaaaatgttt 23340 ctctttacat ccttattctg taagcttttt ttctgttttt aaaaattttt aatttttact 23400 ttttaaattt ttttattaaa aactaagaca taaactcaca cattagccta ggtctacaca 23460 gagtcaggat catcaacatc actgtctttc tcctccacgt cctatctcac tggaaggtct 23520 ccaggggcag taacacacat ggagctgtta tctcctatga tgtcaatgcc ctcttctgga 23580 atatctctga aaggtactac ctgaggctgt tttacagtta acttttaaga aaatataagt 23640 aaaaggagta caccatacaa taatgataaa aagtatagta taataaatac ataaaccagc 23700 aacatagtta taatcaagta ttatgtactg catataattt tatgttatac ttttatatga 23760 ctggcagcac agtaagtgtg tttccaccag catcaccata aattgaacaa tgcactgtgt 23820 tacgacatta tgacgtcgat gtggtttgga tttgtgtctc cattgttgga ggagggggcc 23880 tggtgggagg taatcagatc atggggcaaa cttccccctt gctgttctca tgatagtgag 23940 ttctcacgag atttggttgt ttaaaagtgt gtagcacctc cccctgctct cttttcctcc 24000 tgctctggcc atgtaacaca tgcctgcttc cacttcatct tctgccatga ttttgtttcc 24060 tgaggcctcc ccagtcatgc ttcctgtata acctgcagaa ctgtgagtca attaaacatt 24120 tttttcttta taaattaccc agtctcaggt agttttttat agcagtgcga gaatggacta 24180 atacagacat ctttgtcacc agctgctagg aattttcagc tccactataa tcttatggga 24240 ccacctcatt gtatatgcag tatgtcattg actgaaactt cattatgggg cgtgtgactt 24300 taaagccaca gaaataaatc caagcatctg gcttcttact ggtaaagtga atatatacat 24360 ttcattttca ctttcagtta taatttctaa ttaacactct cagatagaat tctgtagcat 24420 gtatactaga tcaagataaa cagtagaata agtaaaggac tttaaggaaa ttgaaacatt 24 480 ttataagaac tccaagagga aaaaggcagt ttatttttcc atcattttta gtatgagaaa 24540 ataaaataaa tataaattca aagaaaaaac acggaaatca tgctctttga cagtcattat 24600 ttataaactg aaacttaccc cttaacttca taaacttcct agaaattatt gtttcttgga 24660 attttttttt tttttttgag acagagtctt gctgtgttgc ccaggctgga gtgcagtggt 24720 gcgatctcgg ctcactgcaa gctccgcctc ccgggttcaa gccattctcc tgcctcagcc 24780 tcctgagtag ctgggactac aggcgcctgc caccgcgcct ggctaatttt ttttgtattt 24840 ttagtagaga cggggtttca ccatggtctc gatctcctga ccttgtgatc cacctgcctc 24900 ggcctcccaa agtgctggga ttacaggcgt gagccactgt ccccggcctg ttttttggaa 24960 tttttattaa gaattcccca aactcaagaa caagtttcct ggtgtctttt tttttttttg 25020 gagacggagt cttgcttgtt tgcccaggct ggaatgcaat ggcgtgatct tggctcactg 25080 caacctttgc cccttgggtt caagcaattc tgctgcctca gcctcctgag tagctgtgat 25140 tacaggcaca tgccaccatg cccagctaat ttttgtattt ttagtagaga cggagtttca 25200 ccatgttggt caggctggtc tcgaactcct gaccttgtga tttgcccacc tcggcctccc 25260 gaagtgctga gattacaggc atgagccact gcacctggcc agtgtcttat tgatacacac 25320 agattaggta ttgctaccat tgaggggtta aaaaagaagc agggggaaat tttgagtatt 25380 gagtgataat atttgagaca tttttagtaa tagcaatgtg gttgtgaagt gtcttgtcac 25440 tgagatattg atggaaatga ataaacattc tttttaaaaa aatttattct gatcaattct 25500 gtggcaaata agctatggca ggggaatttt gcttaaatga aatcatgctt gaaatatgca 25560 agagatttaa acagggatct cccctagtat atgagtagta aataagtgtt ttgaagtcat 25620 aagaagagtg tcgtgtgcct tctgtttttc tttttatgaa ttctgtttga ttcctacatt 25680 ttttaggttc agcttccttt gttgggatac ttaaaaacag gtacattttg attgcttatt 25740 tcttggtggc cagtgatagt tattcatgtc aggatttagg tggaattaaa gacctcaagt 25800 atctctttca atttgcacag taaaacaggc aagtaagtgt aatttgttcc ttggtgagct 25860 ctgctcttac tgtaacaaga acccagaaag gcactatttg tatcagttaa tcccttgaag 25920 gtagctttta ttttggatag tcaactgggg aatgtaatgg gctccatgct aaagaaactt 25980 tcactgtgaa acatttctgc caaccgaatc tcacttgtag tctttatcat gatttctttg 26040 ctataatttt tacattctga aaataaatgc atttataaag cagaatttgg gtataaacta 26100 agttactagg aacctcacca gtgcatgctt tcactcaagg cccctcctct tttattttag 26160 ctagggatgc agcttacaag cttgggtggc cttgagtcac cacttttaac tcaatttagt 26220 taaagctttt aattttgttt cttattatct ctttgattat cagggtatta tgcgctcatt 26280 atagaagagt gaaaactaca gaaaagttta aagtggggaa aatatcacca ggtgccgtaa 26340 ttactagaag gcaactaaac acatgactgt taacacggtt attttgttta cgtggttgtt 26400 ataaaaaaga ttgaaaagtt gcgttctggt tttgcttgtt taccaggaaa ttgtgacatt 26460 tctctgtgac attacataag taaacattat tttaatgtgt atataatact ctaaagtagg 26520 ttccaaccca tcattccttt ctctaattgt tgattattat aaatagcact tcagtgatta 26580 tatttttgaa taaattttgt ccaaatttat gataattcat taggtacatt tcctaggaag 26640 ggaactaaga tgtctaaagt ataaatattt taatggtgct cttatcccaa aaatgatgta 26700 caaatttaca cttttgtcat ccctacacct tcatcatctc tatgtgagtc ttggtgatac 26760 tgcatcctca ccagtgctga tgtttcacaa tcctttctag ttctatgaag aatagggcag 26820 cagtttatgc caaatgtaat tctcttggaa accacagaga aaattatgag aatggcattg 26880 aagggaatcc cacaaactag gtcaagcaaa acccagagac agtgagtaac cataaaatcc 26940 agaacgtgtt actgtagata ggcaaaagca actgaaatta gcagaatttg tgcaggaaat 27000 actgccagga agaatgagag tgtggaggag ccacagagtg atagcaggtg ttgatgactg 27060 tcatcaggta agtattctaa gaaggaaaga gccctgtttt gagtgggagc taaactcaaa 27120 tagggagtca ttcctgggag aataggagaa agatgttttt gttcccataa gaggcagatc 27180 tcagaaagca catcttttaa aaaggagaga aatgcattgg aaggaagggg ttgtatccct 27240 tggtggcagt atctagtgag gaagctggag gagctcagag atggtgcacg tgccattcct 27300 cgtaagatcc aatggacttc acaaggtgca tcctgacagg cagagtcgga ttcctgcctg 27360 cactgtgcac ttagtgcctg ggatttttct ccttgtatgg agatgcggac aagttaacag 27420 cctgtgtggc tcatctttga ccaatgggat acaaaagttg gcaacacatt cttccccttt 27480 tctctgcctg ggtgtgacct cttgaagtac agtttgtgta ccgctgtgca cttagaaatg 27540 gcgagctctg ttgcactccc ttatattggc tccctccgcc ctccatttct gttttttgtt 27600 attgttgttg ttgctattat ttcttctcct tattttcact tctttctgct ttcctggaat 27660 catacccctc aataaaatac ttttttctgt gtctcatgat gatgtcttac ccgtcatacc 27720 tttattgttt ttctgatttc tattgggatt tctttactgt tccatggatt atttaaaaat 27780 gtattgctta attttcaagc aattgaagat tccctagttg tctttttcgt ttttagattt 27840 cacagccaga tttctttcag agaataaaac attgagtact tttagtcctt ttagttttgt 27900 catgacttgc cttaggccca acatatggtt aattttggca aatgttccaa gaggacctaa 27960 caaaagtaag ttttgccatt gagtacaatg caccttagag aacaggtagg tcaagtttat 28020 tgttttttcc acatctactc tgtccttgtt gactttttct tttactgtgt attctatcag 28080 cttttgaaag agatgttttt aaattacccg caatgacggt gaatttgcct ataagcctta 28140 aagctgtgat tttagaagac tatagatttt ggaatgtgag accttcctgg tagtttgacc 28200 ttttcatagt taagaaatgc acaccatcat ctttgtaata aggctacctg cgttaaagtc 28260 tgctttgtta ttaatatagc tacctcagct tatttcattt aatattgcat gatatatatt 28320 ttctcatcca tttactttca gcctgttttt gtctctgtgt tatgtatgtg catctcttgt 28380 aaacagcatg tagctgtgtt ctttgttact gttttgaatt cagcctgaca atcttagtct 28440 tttacggagt atttagttca tttttattta atgtaattac cgatgtattt gtgtttgtat 28500 tcatcaacct attttttttc tgtttgactc agtttttatg tagctttttc ttcctttgtt 28560 gttattactt agctatttca acatgcatcc tttgcatatt acattctatc taatgacttg 28620 ttttaagaag agataattgc agtttgattt aaacccttaa agaagaataa aaacttcaaa 28680 ttgtttccat gaagtgagaa tgccactgat atctaagttt gataaaaatt tcacaaaaaa 28740 actatttccc aattttattt atgaatatca gtgcaaaact cttcatatta ttggcagaaa 28800 aaaaatccag caactcatta aaaaagtaat cattaaaaaa gtaactcatt aaaaaaataa 28860 tatcatgatc aagtagggtt cattctagga atgaaagatt gtgaactcta ttaaaataat 28920 ttatcatatt aattggtcta aaaatcatat gttaatttct atagatgctg aatatttatt 28980 tgacaaaatt caacagccat tgtggatatt ttttaaaaag acaactcaat aaaataagag 29040 ttaaaacatt ttataaacaa ggtgaactga caaactattt atgctatagg ggcagaaatt 29100 gggtgatccc tttcttcccc attataaagg tcacagccta cacaccctta taacgaaaga 29160 cagattaaca agagaaatgc atagcacatt tatttgatca tagttttatg tgacacagaa 29220 gccttcaaaa atgaagactc aaagactcag ggtaaactat ccattttcat actggtcttt 29280 ttggaatgtt tatggagact tcattagcta ggcatgattg aagcataaat ggccaatgta 29340 gaactgtgct tggcaaaaac ggcatgatct aatgttaata gacctggtag ggaaatctca 29400 acaaggcctg ttcagattct tcttgcccta tctatgcagc attccttcct cccaggtatg 29460 ggatatggcc catctggaat gaagtcttaa tttctttttg gccaactgtt acatggaaag 29520 gtcggggaag ttgagagtaa tatttttagg ttttatgcct ggctttggtg aaaaggggtt 29580 ctggctccta tgacctgcct tgaggaagag ggcttctagt ttccgcagct ctccttgggg 29640 aagaatgagg ctaggggaag aatgaggctg agaggagaga gtaggaggtc agagagaaac 29700 ttgcttctga gactgcttct gaggccttca ttttgaaata taattttttg agccctgaga 29760 atgcacacaa acaaacagaa acacacagca cacaaacaca caatcttgtc tcaaagccat 29820 tttgtaatga ataacaaaac actagaggca tttctgctta ttctgttaaa agtataaatc 29880 agataaggat gcacaattct caaccctgct actgatgctg taccagagat tttagccaac 29940 aaagcagata caagagacaa atgaagtctt aagctctaag acctaagcag gtggaagtaa 30000 aacctttatg tttctgtgac ttaatatttc aaaaaccccc aaataacctg tgagagtttc 30060 taaccattac tcaattccag catctaaaat aggaaaaaca gagataaatc tgactcatta 30120 aaaaaattct ttggtgggca aaacacacac acacacacac acacacacac acacacacac 30180 acacacacac aaaaccaaca acaaaaaaac cctctgggga aattcaaaag aatgataaac 30240 tgggaaaaat atttacttct ccaatcacca agcattttcc ccctaatata ttaagaactt 30300 ttagaaactg gggcgtgtgg ggaagtacaa gctatagagc tgttggcaaa gaacataaat 30360 gaacagttta tataaaaaga attgcaaata gcatttcagc tcataaaaaa gatattcagc 30420 cccacataag acaccagttg ttatttgatt ttatttaaga aatgagagaa tataagtata 30480 tatttctaaa tttgcttgcg ttttataaag aaactttcaa ggaagcacaa gaatgaatag 30540 aaatggttgc ctacagagac cagagtggag ggacaacaaa tagggtgaat gggacaggaa 30600 ttgaagccta attcttacat acatactttc tcatattgtt tcatttttta gccatgtgaa 30660 tatattactt ttatagaaaa atatataaaa atacaaattt gcaattcctt tatttaaggt 30720 gaaaaatttc acctcttttg attactgtgt aagttgagtt ctttaaagtg ttcatgtgct 30780 atttgtattt tcatttctta aaattatctg ttcaagttct ttatatttag agatattttt 30840 gttttgttgt ttttttaacc aaactttata attccttaat atgttaagga accagtacat 30900 tttatcattt ttatttcacc ttttaaaagt ctggttatcc tatcattttg tttattatac 30960 attaacttag agaaattaat aattcttatt ttgtcagatc tatcaatctt cctgtgttga 31020 ctttttaaga aacattacta tattttattt ttgaaatgac acccaaatgc taaacgttta 31080 ataagaatca tatcaatttt aaggaaattg attatcactg gctgaccttt cacttcaaac 31140 tgccctcttt ttaataaaag cataaaacat ttcaaaggga tcatgacagt gtattttatt 31200 ttatgaccta gtatttattt taatatatac taggtctagt tcctctttat tccttttcta 31260 tttaaaaatt attttcacca gtagatgttt ttggatgaac ttacggatca tagtgtcaag 31320 ttcgcttctt atgtcaattc ttatatgtca atttcacttc caaatttctt atgaaataat 31380 ttggaaaaat gatatttctg taatatatag tttccatcta ggaataaagt atgcttctca 31440 gattgtataa ttgttctttt tgcctttcaa tctagtttta ttgatttctt tatatatttc 31500 catacattac catattcatt cctaactatg gtataatttt gttgctaacg tggattaggt 31560 aatttcccct acatattgat gagacatgga atgatgaata taagagttgt tgatattatg 31620 atgtatatat tttggtttct gtccatggtt tctgtctcat aactcccata gaccttgtta 31680 tttcctaggt gactaaaaca ataagtatat gtcttttgtt aaagtattta gtcttttgtt 31740 cttggtttct gaagcagctt ccaaacagct tttaagtaat aaaggtgaaa gacagccttt 31800 tgtcataatg ttggggctct ttagggctca gaagcaggac tcagaaaaca gaatctctct 31860 cagactcatc ctttttattt attatttatt tatttattta tttatttatt tattttttga 31920 gacggagtct cgctctgtcg cccaggctgg agtgcagtgg cacgatctcg gctcactgca 31980 agctccgcct cccgggttga cgccattctc ctgcctcagc ctcccgagta gctgggacta 32040 caggcgccca ccactaggtc cccgggcgtg gtggctcacg cctgtaatcc cagcactttg 32100 gaaagccgag gcggtcggat cacgaggtca ggagatcgct ccccaccttt taatttaaaa 32160 ttttcaattt tataagacag ggtctcactc tctcagccag cctggagggc ggtggcacaa 32220 tcttggccca gtgcagcctt gacctccggg actcaggtga tcctcccatc tcaacctcct 32280 gagtagctgg agctacaggt gcacaccacc ctgcctggct aattttttgt gttcttttgt 32340 aaagatttag tttcaccatg ttacctatgc tggtctcaag ctatctatct atctcagcct 32400 cccaaagtgc tgggattaca ggcgtgaccc accccacctg gcccttctgc ttccccccac 32460 tttttttttt aactttctcc tttttctccc caaggaaggc catagaaact aaaaatatac 32520 tctcatcttc ccccctttgt gtcttggagc tggccgtgaa gaaattgtct gattgtaagt 32580 cataagatcc ccatttctga aggggtcctg caccataccc aggaagaagg aatgctgcac 32640 agagaggcca agaaaaatct aaacagtcct tgctgggttg ccccactcag cctattcata 32700 ttaggtcata ctctctttgt ccagtcatat ttttacatga ttgtccatgc ttcaatcatt 32760 cctatccaat gaattttcca taagaggccc agaaagatag aatatgggag ctcctgaaca 32820 gctgaacttg tggaagcttg cagaaaggtg aagaagaact catccacgtg cgggtgggtg 32880 gcacatccca tttacacagg aatggaagtg cttgtgctca ggacccttta gacctcaccc 32940 tatgcatctc ttcatctgac tgtttatttg tatttttaaa aatatccttt ataataaagt 33000 tgtaaatgta tttctctgaa ttatgtgagc cagcctaaca aattaatcaa acccaaggac 33060 cgggctgtgg gaactctgat ttataactga tcggttagaa gcccaggcaa aataacctgg 33120 ggcttgtggg aactgagcct ccaacctgtg tgatctgatg ctgtctccag gtagatagtg 33180 tcaggatgct atttccaggt agagagttgg gtgtccactg cagaactgat tgcttgcttg 33240 ttggttagga gaaatcccca caaacttggt cacagaagtt ttctgtattg attgttgtgg 33300 tgtgagagta gagaaaaaac tgtttttcca cccagcacct atggtttaat tcataccttt 33360 ttttttcact ctgtatgcca cttactttaa aaaaataaaa atgtgagtgt cttgtctggc 33420 cagcaagatt aagaaataag tttgtcttct gctttccggg gctctgttta ttcagttata 33480 ggaaggaagg gtagagttga atcaaatatt aattcttaaa acatcttttg gttttttatt 33540 tcttttataa tgtgaggtaa tgcggggtgt atttattttt ttcttggtat cctaatgtat 33600 ataatacttt gaaagttgtc ggaaccctct atggattaga catacatgca tagataaata 33660 agcatgaaca tcactgtcgt catctaccat tagtgagctt atttatttaa ttctttctct 33720 tattttaatt ttctggtaca ataggttaat attctgtgaa aaccaacctg acattcttaa 33780 ccttttcatg ggacattaaa ctttgactac tgtcacagtt agtaaagttc cctttacaga 33840 aatttaattt gtgcattaaa atgtatgtat acaattatct tttattaaca cattttacag 33900 ttgtgattaa atcactgtat aattttatta aaacatatga aatgattgat tttgagatag 33960 agataataca tagtgccaca tatggcggga aatacacgca catccttgtt cagaagtatt 34020 ttctttagga tggaaggccc tttgagagag acactgcagc tctcctgtgc ctcagcctac 34080 ctccctgggc caggtgtctc tgatgtggtg ggttcagctt cctttatgcc acttggccac 34140 tgcctcttca gtgcaagcct ctgactctct cctcagctat cttccatgct ccctgtgggt 34200 tttggcagaa acttctggat tccttccaga gaagaagctt agatgagtga actcagtctt 34260 tctctctgcc tacctagttt tcacctttgt tgtcacattt ggtcgtttct tgagtgagac 34320 tacccttcat gttggtatct gtcaggagtt tggctgggtt gttggggact aagcatgcca 34380 ccaaatccac tctcaatccc ctactggtgt gtcagtactg gatgagaatt tagctcacat 34440 aggtcttcct cttccttttt tctctttgcc ttcccaccca ctagctgata tgccaacttg 34500 catcacatcc tttttctcct aagggcaacc tcctgtttag cctccattga caaatcatat 34560 ttccattctc taccatggta gaaactcaat ttagaccaac tttcattttc atgattgaag 34620 ccttaggaaa gctagacaat agggatttac aaacaggaga ttacactggt ttattctaag 34680 aggagcctaa cttcgtgcta aaaacgtgaa tgcaagacag gctgcctagt ttcttgttct 34740 gggctgtcac ttactagatg tgtcacttta ggtaaataat gtaatttctt ttttcctcag 34800 atttgtcatc ttaatggcaa tctgttacaa caactttata agaaagggat tattttatca 34860 tcattttatg atgataattt aaatgtcatt taaaatgatg acaaaatagt ccctttctta 34920 aaaagttgct gtaacaaaaa attagttaag aacctaaaat agtaactgaa aagtaataaa 34980 tatttggtat ctgttagcta ataccaaata ttaggaggct aaatgaaaaa tgtaaagttg 35040 gaaatgcagg tgcctaatta agtgagactt tgaaagtgcc tggaatttag acacacaatg 35 100 tagcaagcaa caagaagcct tgaaataatt tgagtggttg tgtggtgaaa gtagtgttga 35160 cttaaattag ttcattagga gtatattaag agtgagacag cgattgtatt tacaggactt 35220 cagcctcaag gtgatgaagg cctagactaa gttgttgcaa atagcagtgg tgataaaaga 35280 aaacagagga gattatcagg gatcagagag aggtgtgggc ccagggtgtt tctgagccaa 35340 ggataatggc tgcatcatgt agaatttagc agaaatagag agttaaaaga agagaatgtt 35400 gtctgggtag aaaaattttg attttagatt tattaagttt gaggcaataa taggctattc 35460 aagtggaagt atcccagggg acagggattg gcaaactttt tctgtaaaat gccagatgga 35520 aaatatttcg gtctttgtgg accagatgtg gtctctgtca catcttattc tttgtatttt 35580 ttttttttta actattcttt taatatatat ttaaaatgca aaaatgtagg acaccaaaaa 35640 aagctgcaat ctaacttggc tcatgacaga gccttgactg agatattggg tctagaaatc 35700 tagattaatg ttcaccgtgt ggataatttg aaaactcttg agtaagcata gatgtacagt 35760 attctaatta agatagatta ttattgtaaa ttatataaat atttacattg aaagtgtttt 35820 gcccagaaat agattttacc ttattttttc tgagccctgt aaagttacta aggacttccg 35880 tcaactagat ataaaaatag attcaataaa attaataaaa tttttcaaat gttgattagg 35940 atttaagaaa aatagtttca gaaaatttct aatttcataa ttctgtggaa attaatgcaa 36000 aataatgttt ggcttactgg atgtcacaga ggctaaaagt aatctttagt ttatgagagt 36060 atcatgatac atgatagaat aagacaggct ataagaattg tagccagctg tgctggcatc 36120 aaggaattag aatatttatg ctttaactat gggattaaag ggggttatag cctttcaaaa 36180 ttgacttaaa taaaggcact atagatagaa ttaaataaaa acaatatgag ttccttactt 36240 ctgtccaaat taaccaaatg aatggcacaa actgaagaag tctcttaaac taaccataat 36300 taatgagttt atttattccc ttaattcaag tagatcacta gagtttaagt ctattttcct 36360 aatggaatgt tattgatttt aagtttaacc aacatgtttt gctttttaca acagatttat 36420 agaaatattc aatattttta ctagtacaac ctagaaatta ttttacttct ttatttaaga 36480 tgatatgttt cagataaact tttgaaatct atagagtatt actttcatat atgtaaatgc 36540 agagtgcaga ttacagaaat tagttttaga ttcaacatta tgtcgaagtt ccagtacctt 36600 tatacttgaa gtgtttgaga gaaacattgg caaattggag cttacataga aagacacatt 36660 caggaatata acatatctaa gtactaattc aagtgaaaaa tagctaaaac aactagtatg 36720 gagaagaaag acgttggagg gtctgggctc atgtatgcat tgcacaatgg ggtgagttgt 36780 gtatatatca cagttgtctt aaatatttaa aatggcagaa gaaggagcag actcatttat 36840 ctatccattc atccatccgt ctaacaattc acccatcatt tcatgtagaa atagccttct 36900 atatgctagt cttaatgtat atactagaga cagaaagatg agtaatataa cccctacgta 36960 aagaagctca tagactaatg aggaatctca tattttgtgg gaacacttat tacttaggta 37020 aaccattagc tttcatttcc tcctctctaa gtccagataa taataataat acataactca 37080 gagggttatc tatgggggaa aatgaaattg tgaaaagtac tacaaaaatg ctatagaatg 37140 ttagatgaca tattattgac tgagggattt ctggataaga gattactaac tcatgttttc 37200 tgaatctcgt gatttaaaat tttgtagtaa attagttatg tgttgtgtga taaaccatat 37260 aaaatttagt gtcttaaaat cacaacactt tattgtttct cattgtttta ctggttgact 37320 gagtgatttt ctccctggtc ttgtttgggc tcattcgata tctcctcact cacctgtctg 37380 gtgcctcggt gatggctgtt gtgtgagccg ctttcatcat gcagtttctt cattaggtag 37440 tccagctgga ttttcttaca tgacagcaga aacagtcaaa gatgtaacaa cagaatggca 37500 aggcctcttg ggacctagag ttggaagtca cacattattt ttgccatatc ctgttggtca 37560 taactagtta taaagacata ggacataaat cgtagctagg acataaatca tagctagccc 37620 agatttaagg ggagggaaga gagactccac ctttcaatgg gagaagtggc aaattcacac 37680 tggagaaggg tgcatgggat ggaagagact gttgtggcca tctttgcaaa ctatctacca 37740 catatacaca tggcaggttt ttatttccca tttggtaaag tataagtagt ctatttttct 37800 gtgtacagtg gctaacagtt gtacttattt tgttgtgtgt gtttcaatca tcattactag 37860 caaggaataa tagtatgaaa aggcacagtt tgagtccttc tatatagttc atagtcattg 37920 ttgtttgtaa tcaaagaaat attgcagcaa cttctaacca ataggttatg agtgaaggat 37980 ttgtctgtct gtgaaaaaat ctagtagttt gaaggttctt aattcttgtt actttattat 38040 tacactgtct tcattttttt agcaacaaga ggtaaatttt tactttactg cttaatatct 38100 gtcactttta ttcctttata acaagaagat aaatatttta agtcagtttt cctcttatct 38160 aagtatataa ccaatggtta cagagcatgg agttggggtg aagtgtgact ttgttttctt 38220 tttattttaa tcaagtagtt tcaatatcat ttctaactag acagccttac ttttcaaaca 38280 ccgcaccgca aagtattatg ttgttgcatt gatttatttc tgttgttttg aagtctcagt 38340 gaatttataa tttatatccc acactatctt gagggtatgt gtgctaaagc agaaaacaca 38400 aacttgtcca ttttggtaaa ataagggtat tctaaacttt tgagagttgc aagaatccta 38460 ctttgaaaaa tattcagtct ccagatgaac tgtagacttt aaatacgtaa aagcattata 38520 acaaagaaaa acataccagt ttatagccaa tgacgtatgt tgatcgtaga ctctagctat 38580 aggaatgcct ttcattttct tgaaaataca agacagtttc ttatcttttg acttatagat 38640 atgttgccct ctcaggttga agcgcaccct actattcttt tgatgagtgt ctccctacct 38700 gtaagtccca gtttccatgt cacttcctcc acaaagtctt ctggccaggc acctctcttg 38760 catgtttgca tagcaccctc cgctaatgcc tcccagtatc atgtagctgc tgtgagcttt 38820 tctttcttcc tcactagact atgagctcaa taaggagagg cgtgatgctg actttgacca 38880 tgtttatacc ttcacttctt agcacagcag ttagaacatt gtaatgggca actgatattt 38940 gttaaatgaa ttaatttggg gtagaatgta ataaaatttc ccactttagc attttcattc 39000 agataaaagt acaatagaaa atctcatttt tgaatcagga ctcataattg tttggcattg 39060 aaatagaaaa aacatacaat gacctatctt ttcattgtgt acaacagatc aaacattctg 39120 ctttgtaagt atctaaatag tctctcatta aatggttctt tatatgatca aggatatgat 39180 ttttcttgat ttacttttat tttgtagttc cctagcaaat tactatggga attaaggtca 39240 cacaaatcaa atatctcaat tatgcctttg gtatcttact catcactctc ctacctactt 39300 tgactatatc tttctgcttc ttaaaatttt ctacagcttc cttttaccct ctgaaacaat 39360 aatgatcata ttgccccact gttctcagag cgcttggcta tcagatacaa ggtattcgac 39420 tgtatctgcc tccggaacac acgtgaaata atgtatttat tactatctga aaatatattt 39480 caactcacct tctgtagttt gccacctttc cttaattttt gtccatttat atcatacata 39540 taaactactt tattcattct gacagcagta gcatagattt cattcttttt aatttcatta 39600 tatggtttgt ttcctccaaa aacaaacatt ctagtttttt ataccatttc taatattcca 39660 gcataattaa tgatctgctc ttttcattca acccatattt gtactttgat atctatgaat 39720 tatttggacc agctaaacaa tgtaagcaaa ttgaacattc acagcatttg atcttttttg 39780 tctgggtctt gatatttaga aagttcagct aaagttgata ttaaagtgtt aaatttgcag 39840 aagtttcagg taactcttca gttgatcttt actgaaataa tgaaccacag cctagagccc 39900 ttgtgctttt gtatgcttac taggattcca taatttttaa ggatatatag ttggaaattt 39960 tgggaagtgg ggatttggtt tttggttttt ttattctcaa ctgaagtgta gcatgtatac 40020 agatcacagt gtaggatgca aatacagatc accagacaga accttctgtg tgctttgtgg 40080 agtcatttga ttatttgtgt agtatctcac atgagactta attttaattt aaacaaacct 40140 gtaagtctgt tcaaagtaca gggaaactga ggtagaagta ataccctaac aattttgcag 40200 agaatttcag aaatataaaa tgtgtcaata caaaaattag ccggatgtgg tggcacctgc 40260 ctgtaatccc agctactcag gaggctgagg caggagaatc gcttgaacca gggaggcgga 40320 gattgcagtg agccaagatc acgccactgc actccagcct gagtgacaga gaagacttca 40380 cctcaaaaaa ataaaaaaaa aagaaataaa aaatctctga agtttcataa aataaaatgt 40 440 ttaaaggtac agtaacagca tattaattta ttgctgtatg ctaaatagtg tgctaggcat 40500 attgcacaca agattatgta tgcaatatgt atgcaatgtg cctcctaaaa attgttgatt 40560 atttgtatta ttatctctgt ttttcagcag ctctggaagt taaataaatt accctatgta 40620 ttagttttct attgctatgt aacaaatcac cacaaactta gagaattaaa ataacaccat 40680 tacttatatc atagttctgt agatcagaag gccaagcagg ctggactggg tgttctactt 40740 ggggtctctg aaggtagaaa tcaaggtgtc agccaggctg ggctcttagc tggcagctgg 40800 aaagaattca ttatcaagct cattcaggtt gttggcagaa ttaaattcca cttgaacctc 40860 gaacaacatg ggtttcagct gcaggtccac ttatacccat tttttaaaaa taattatatt 40920 gggattcttt tggatatttg agacaatttg aaaaaaaaaa accagatgaa cagtgtagcc 40980 tagaaatact gaaaaaatca ggaaaaagtt aagtgtgaca ctattctgga gatactagtc 41040 tcttttatca ttaactacca taaaatatac acaaatctat cattaaaagt taaaatgtat 41100 cagaatttac acatataaac acttagagtt gacagaaatg taaacaaata tatggaggca 41160 taaaattaat tgtggtacat actaaactac tgtaataatg ttgtaacccc ctcctgttgg 41220 taatgaggtg aactccagca ctataagtct ctgcataaaa tgctctgtga tgctaatcaa 41280 cctgtgtgag cagctcatct ccagtaaatt gcatactgaa gtaaaaagtg atctcttgca 41340 gttctcgagt atttgttata gtgtttagtg tactaccata aaccttgaat aacaccctac 41400 ggcccacagg aagtgtcact agtgatgctg gaagtgcttc caagaaacag aaaagtcatg 41460 acattacaag aaaaagttgg attgcttgat atgtaccata aaatgaggac tgccactttg 41520 attgcccatc atttcaagat acatgaatcc agcctaggaa aaggaaattt ctgaagctgt 41580 agctgcagct acaacagcag gtgcaaaaac cttgcacatt ttgtgaaata catttgtatc 41640 tcatatcgaa aatgtagctt ttatgtgggt acaggattgc tatgagaaag tcatacctat 41700 agaccaagag acagcatact agtttcctag acaccattaa aaatcattaa atagaaagga 41760 tatctgcctg aacaggtttt taatacagac aaaagtgtct actctgggga aaaaaagcca 41820 cagaagacat ttattagtaa aaaatagaag ccagcaccag gaattaagct ggtctttttt 41880 gtctgggtct tgatatttag aaagttttta aggcatattt agaaagtttt taaggcatat 41940 ttagaaagtt ttaaggaatt aaggcaggaa gggataggct aactctactg ttttgtacaa 42000 atgcagtcag gtttatgatc aacaccgccc tcatctataa agctgctaat ctaagagcct 42060 tgaagggaaa agataaacac cagctgccag tcttttgctt ttacaagaca acctggacaa 42120 tgagaagccc tattctgcat ttgttttatc agtgctttgt caggaggtac cttgcaagta 42180 aaattctttt gattttggac aatgcccttg gccacccaga actccatgaa ttcaagatcg 42240 aaggtgtcta agttgtctac ttgatcccaa acacaacttc tctaattcag cctctagatc 42300 aaggggtcat aaggacttta aactcattac acatggtact ttatggaagg gattgtcagt 42360 gacatgaaag agagccctga tagaaaggac atcatgaaag tctggaagga tcacacaatt 42420 gaaaatgtcg ttgttattga acaagccatg aaggccatta gcccaaagca ataaattcct 42480 actggagaaa actgtccaga tgttgtgcgt gacttcacag gatttatgac agagccaatc 42540 aaggaagtta tggatgagat tgtaggtatg gcagaaaaaa aaaaaaaaga tggggtaaag 42600 gatttcagga tatggatctt ggggaaattc aagagctaat agaaaccaca ccagaggaat 42660 taacagaaga caacttaatg gagatgaatg cttttgtgcc agtgccacac tatgaggaag 42720 aagacatatt ataaaagaag cagtgccaga aaacaaattg acattagaca atctggcaga 42780 agggttattg ttattcaagg ctactttcaa cttctttcat gtcatggacc cttctatgat 42840 acaggcactg aaactaaagt gaacagtgga agaaagatgc taccacatag aaatatttta 42900 aagaaataaa gaagcaaaag agtcagaagt tacaatgtat ttcctcaaag ttacaccaag 42960 tgtgcctgcc tctcctttct ccccttccac ctcctccact tcttctgcct ctgccacctg 43020 agacagcaag accaacctct ccttttcctc ctcagcttac tcaatttgaa gacaatgagg 43080 atgaagacct ttatgatgat ccacttccac ttaatgaata ataattatgt tttctcttag 43140 gattttctta ataacatttt tttctccagc ttgctttatt gtaagaatac agtacctact 43200 acatatgaaa tacattcaaa atatgtgtta attggctgtg ttatcaataa ggcttctggt 43260 caacagtagg ctattagttt tgggggagtc aaaacttaca ggtagatttt tgtctttgcc 43320 ggggatcagt gccccagccc gagttgttca aggagctgta cttgcagttg taggagtgag 43380 gtaccagttt tcttgctggc tgtcaacagg ggctgctcac cactcctgga agctacctgt 43440 gctccttctc atgtggctca ctcacttcaa accagcaatg gcatgttgaa accttcttat 43500 gatttgagtc tttctggctt tcccttctcc taccagccag ataaagttct ctgcttttaa 43560 gggcttgtat gattagatta gtcacccctt ctatgtaaaa tctctctatg ttaaggttaa 43620 ctatgccatg taacatgaaa caatcacagg agtgatacgt tgtcacatgc acagttagtg 43680 tgagatattc tgtgggagaa ggggcataat tctgcttacc atatcatatt gctcaaaaca 43740 gtaattggca gagttagagg tcaagcctgg gtttggttga actccagagc tttgtctcta 43800 taccacattc atcagagggc ctaattttat ctgtggtgat atggaaacca gtcttagtcc 43860 tatatagcca ggctatgttg gtcccatatg ctgctataaa tatattgcct cagaggtctc 43920 cagcacagtt ttacatgcac actggtcctg taaaatagga gatccagatg gtcagaaatg 43980 catacgttag gttttgttat gccccatata tttctgtgat ttgcacttgt gtttattagt 44040 ttattctgcc ctattttatt acaagctttg atttctactt tctacccttt ctcccttctg 44 100 cttaccttat gaacgtactt aaaaaacttg gttaactaag agtcaggctt attgcagttt 44160 cttttggaaa ttcgtagtca tatttttttt tctgcttaat taaatccttt tcagaggtaa 44220 ttgagttgag tgaccataat gtgctaaggg aactgcaaaa gataacaaag tcaatcaaga 44280 gaaatccctt acttgatcat acaaagctat catttaacta gggaccccat ggatttttct 44340 gaagcagaat atttgatctg gtgcacacaa tgaaaaaaaa aaaagcaaat gtgtgatttc 44400 gttgattttt tttttttttt ttgacagagt ctcactctgt cactaggcta gagtgcagtg 44460 gcatgatctc agctcactgc aacctctgcc tcctgggttc aagtgattct cctgcctcag 44520 cctcccgagt agctgggact acaggcacgt gccaccacac ccggctaatt tttgtatttt 44580 tagtagagac agggtttctc cgtgttggcc aggatggcct tgatctcttg accttgtgat 44640 ccgcctgccc aggcctccca aagtgttggg attacaggtg tgagccacca cgcccggcct 44700 cattgatttt aaataacata aacaaatata tgccgtatat ttcttaggtt gttaagtgta 44760 acatattttg agataactat taatttcttt gttaacagct tttgaatctt gtttgcttgc 44820 ctccattaag aaaatgacac agtcttcttt ttcctcatga ctcaccaggc cagagacagc 44880 ctcccagatt acaactagct cagtggtatt aggagcagta tggattgtgt atgaaacaaa 44940 gtccaacttt tatttactca atgactaagt aatttgggac aagtcactag atacagctgg 45000 acctccttat tctcaattat aaaaataagc aagccgttcc acagttctat tattttaaat 45060 gacatctgct atgatatact ttgcttagta tccaagaggc tggaaatatt taatttattt 45120 tagtttgacc actaagaaat ttgttctctg gatctcataa agacatggaa acattttaca 45180 ggtgaatatg tgtaaatatt atagtaaaat taatttgaat actcatgggg cttgtcatac 45240 ttaatcaagt tataagatta ttttccttct ttacacaata aaattatttc tttggtttca 45300 attttttgta tactcttgtg atctcaaagg tgttcctttt attctctttc ttttatttaa 45360 ccagaaatat cttttatttt gaatgctatt ttgtcataat tcatgcatga ttgtgcttac 45420 tttaaatggg acatgattat ttctgctgtc taggatacat gaccaaaata ttaaaaaaca 45480 gataattgca gattatggcc aacatggcta cacaatgtct tagaaaagtt agttttgcct 45540 ttctttgtga aatatattgc atgaaacact gatgttatca ttttgatgga ttctccccaa 45600 aattgatggc ctggttttta caagtcaaag catctacata ttttaaagtg ttaaagaaaa 45660 ttaaatgaac agttaaatta gaaatggagg atgagctata acttctcttc ctagaaggga 45720 ggacattatg agcaacatgc acatagggag tgatttttct ggcaataaag tttggaaaat 45780 agcaaccaaa acagaacaaa actattcatg ttgtcattca gtacgtagag aacaaaaagt 45840 ttccactata caagaaccta tactactata tatttgcaat gatgctttaa tgcaggggaa 45900 aacagctgtt tatctgtttt tcttttggtt ttattttctt tttaccttgc catcctgtca 45960 gtacgtggct tcataaagct gtaggattct gatagggaaa ctaccgattc catgtgcttt 46020 ggaagccaat gtgctgatta tctagcttga tagcagtatt ttattatagc tggagggaaa 46080 gtgtggctat gtttgtgaga ggcaggaagg caaatgccac caataacgtg tccagaagtg 46140 agctatcccc tgtgttttat gcacttcatt ctactgaatg aatattgtat cactgttttc 46200 atttttttgt ttgaatatca gagacttttc caatggttct gtttctgtcg aacacttgat 46260 ggaaagccct gttgtaaagc ttccttcatg gagatggctt tatgtcttgg ctataaagta 46320 aagttccata aatttgatca gaaattgtct ttatgggttg ccaccctgtg tgctattaaa 46380 agctcagaac ctgggtgtta gttttcactc agagctgtgc ttgccttttg tatttggcaa 46440 gtgcagctct gagttaaaac aaacactttc ctttatttta ccactttctt cctacagaac 46500 atctctccca ccagcctccc ttttggggct caatagtctt gttcaaccca tctttactgt 46560 acactagagc caagttcttt gggtgttttc ctaggatatt tgcaacatca aggtcaaaat 46620 gttctttata ttcaacaatt cacctctttc attaccctag gtagagctcc tcagaagtac 46680 ttcccaccaa ggagtcctca ataaatatta attatacatt ttggagagat ttgtattgca 46740 atgaacatgc ttcagtgcca gttcttcctt actaaaactt tttgttctcc acttacattt 46800 tgtggccatg tcatcctggc tttgtatcct tacctgctga cgtctatata ttcagcccag 46860 ccaatatgca tttatattct ctctgtagtt ctgagactca ttaatggtcc tcttttacct 46920 taattgagta cagtctgaga gagtgcctgt cttccattat tgtacatttt tcttctcaca 46980 gaacaccttt tgattcttgc tctgatctct tatataagat gcgctgtgaa acataatttc 47040 cttccttcct cttttttttc taataagttg gcatgtttaa ttaacctgtg taaaaaaaaa 47100 aaaagagaga agactttttt aaagggtaat agaagtcata gtaatatttt gctttgcctg 47160 aaaacagctt tctcagtgtc caaagggatt ctaaactata catgtatttc ttcacatgca 47220 ttatttattt aaaaataaat aaataaaagg gtaagtggca gtccagacag gctgcttcac 47280 tgtgtcttga tgcctcatga tagcacaggt tctagaatgc catgagtggg aaaacaattt 47340 ctttcatctt ttctgataat gttgtttgca tgatgtaaat attcagttct agcatgacag 47400 tgggtacctg agggaattta aagtgaaata acaggattgt taacaaattt tcattcccat 47460 ataatttatg tttcatatat ttctactgga aaactaagaa aaaatttttt cccaaaggca 47520 attattttgc aaggcctttt taccctatta taagactttc ttttcagtta ataacccaaa 47580 gtgtctcttc agatgcttaa gtcaagtcgt taattggctt gactaagtgg ttgttggtgc 47640 agaaagctta ttctctccgt tggttgtata taactcacag acattaaatg acccactttg 47700 ttgttcctaa ggcaaagaat cattattttc tttcggcagt atactgtgga cttttaattc 47760 acaaacttat agctcaggtt gaagtaattt ctgctgagaa gtgatttttc taaacaattg 47820 tttaatggtt ttgatttgga gtaaacatta gatgcaactt ttgagctagt atactctact 47880 gtagagatca ttaatgtaac cttttgtttt tgtagcgtgc ctgtctccct tcaaaaattg 47940 tttattctaa actgtgatct tttttctaaa gatttgttta aagttaacaa ggtaagtata 48000 accattaagt tcacatataa atgattaata cacaaatatt cagctttgtt ctctgcataa 48060 atatctacct ctccttaaat taaaatttgt aattgttgaa agaaaatttg aatagtagga 48120 aaattttact taatgatgtc taactgtgaa tttgctttgc tgagtgggaa atagtgatca 48180 ttgaatctca taaataactt tacctagagt tacataaacc ttttgaccaa aattatttaa 48240 agaataaagt actagctaga atatatctgt cagaaagtca ctactactta cttctgcatg 48300 tccataacac acataagtaa tatatacagt cacattaaaa aatgtgccaa ctctataaga 48360 acggagactt ttttctcttt agttctctca gtggcacatg aacacatgta catacatgtg 48420 tacacataca cacacaatgg aaagccaaca ctgaaacctt ctctggggag ttggggaacg 48 480 atgagagaca atgtggtata ataaaaacaa tagggggagg ccgaggcggg tggttcacga 48540 ggtcaggagt tggagaccgt cctggctaac acagtgaaac ccggtctcta ctaaaaatac 48600 aaaaaaaatt agccgggtgt ggtggcgggc gcctgtagtc ccagctactt ggaaggcccg 48660 ggaatggcat gaacccggga ggcagagtct gcagtgagcc gagatcacgc cactgcactc 48720 caacctgggc gacaaagcga gattccatct caaaagaaaa aacaaacaaa caaacaaaaa 48780 acaatagggg ctgggtgcgg tggctcacgc ctgtaatctc aacactttgg gaggctgagg 48840 caggcggatc acgaggtcaa gtgatcaaga ccatcctagc caacatggtg aaaccccgtt 48900 tctactaaaa atacaaaaat tagcccggcg tggtggcagg tgcctgtagt cccagctact 48960 cgggaggctg aggcaggaga atcacttgaa ccgggtaggt ggaagttaca gtgagccaag 49020 atcatgccac tgcactccag cctggcgaca gagcgagact ccatttcaaa aacaaacaaa 49080 aaacaatagg ctgggcgcgg tggctcatgc ctgtaatcct agcactttgg aagggcaagg 49140 tgggtggatt gcctgaactc aggagtttga gacccgcctg cgcaacacgg tgaaaccctg 49200 tctctactaa aatacaaaaa attagcctgg catggcggtg tgcacctgta gtcccagata 49260 ctcggaaggc tgaggcagga aaattgcttg accctgggag gcgggggttg cagtgagccg 49320 agatcgcgcc actgcactcc agcctgggtg acagagtgag attccgtctc taaaaaaaaa 49380 aaaaagaaaa aagaaaaaaa aacaatagtg gactgtttct cctcttgttt gttatagtaa 49440 ctgcactgtg cgaacctgga acaagtcatg taataacttt tggctcactt ttatcttgtc 49500 catatattag gaaaacattc tcaagtgtaa tttcacatta ttattagaat aacagttatt 49560 tgaagatgga gatgatgtta aagatacaga aaagagcttt aggtcgggga gagagcagta 49620 cagatgttct ggggaaaaat ggcattgctc acatctactt ttatctacag caaggattct 49680 aaagggattt ataacccaaa catattgcga atggatttgt agagagatta ataactagct 49740 ttagtagaca ggctgaaatg tagacacaat atgaatactt gatacatagc agggttttct 49800 tcgccaaaaa tacacttatg gaagcaacag taacagagag tgctacaatg caggtgaaag 49860 accctgttgg ggatatgtgt tggagaagct gggagatttc atgctatgac ctcaactaat 49920 tacgacccag cctgtaccca gacagctaaa aattaacaca gaagtttagc atgtaatcaa 49980 ctataaattc tcactcttca gtactcagta gtcttttttt tctgttaaaa gattgaccac 50040 tgatgaccag gaattgtaaa tgaatttatt caagtagaag ggctatttat cagattagga 50100 tcttagttta tctaataggt aaacgactgg tctgagtttt tatactagct gatgttaaaa 50160 agtgatgtgt aaattttttt tctcttcagt tatgtattta attactgact aatccctgtc 50220 aagtataatt gttaggcagc atcactgtct aagaatctaa agtgatatcc cattgccgag 50280 caaatcatat cagattcctc cgtttggctt tgaaggacac atgcaatctg gcttcaacct 50340 gcctatccac atgtatttcc tttgacttcc ttcaatactt ttcattcagt ggcacctctt 50400 aggttttctc atacttgcta ttattattta tgctaggtct atattagctt ggaatatcct 50460 ccttctttct attcaaactt tatccactct tcaaaactaa cctcaattac cacccattcc 50520 aaggccttct ccatttgcag cagtgttgat tcagtattat ttattattta ttttttcatt 50580 tattcacaat aactcaacaa atattgagtg tcttctatgt tacagatgct gtcctcacct 50640 aaacatgaat ttttattgca tgtagagtca taaccaagta gtatactgag tttgtagttt 50700 aatatttatt gtatctcctc tataaaactg agcttacagt ggcagtgatt atgtcttttt 50760 tttttttctt taacaaagag gtcatgaaat agtgtaagtc aaaaccaagt agggaattac 50820 aaggctgatt tgtatttcct cttggctact acttagtcat tcgatgattc aaatcaatgg 50880 acatttatta aacttctgtc atgtacgttg cgatgttata cactatagga gatacataga 50940 caatcactta atagagtcag tattccaaaa gagagagtct ctatttgagg tcctaggacc 51000 cccctagaga attcaatgtt ttaacttcag aggattttat ctaaagtttg gatatgtgct 51060 tatatgcatt tttctgagga gagggtgcat agtttttaga tgttaaagat gtaattgata 51120 attttttgaa aaggttgaaa agctcatgat ctcagtatgt atgaaacaag tacacaacta 51180 aagaacaaga gtcataaaag agcaacaaac cctaagctgt gtttagaaag aggtaaaatg 51240 atatctgact gtggtcattt aaggaaaaac ttcctgaaag agatgacttt tggaatatat 51300 gccatgcatt ttttggcctc agctccctca tggaaaaaaa ggtagattat aaaatctctg 51360 ctattttttc cgagatacaa atttatttct tgattcaatc accaagtgct cttcagaatg 51420 ccagtgacat cataggtact cagaaaacat ctgttaattg gtaaatctga aattaaactt 51480 cagaaattta tagctacaaa tatatctatt gagttgaaat tttagaatat tattgcatta 51540 gcttcctaat gttccatgaa tgaaaagatt tatcagatgt ttatatacca ttaatcacaa 51600 tcttccactt ggcagatcag aatgtttaat tcattatttc tccttcattt cttcataaca 51660 tttaaaaata tctttgtatt ttgttattct tactgttaca gccattgggg tcgcctcatt 51720 agtctttatg atttgtaggg gcaggaactc tcttaaacct gtttgatatt atttagaacc 51780 tagcacatcc ccctaatcaa aaaggggcac agtaggctgg gcgcagtggc tcacgtctat 51840 aatcccagca ttttgagagg tcgagacggg cagattacaa ggtcaggtgt tcgagaccag 51900 cctggccaac atggcgaaac cccgtctctg ctaaaaataa aaaaattagc caggcatggt 51960 ggtggtcacc tataatccca gctactcagg aggctgaggg aggagaatcg cttgagcccc 52020 ataggcagtg gttgcagtaa gccgagatca tgccattgca ctccagcctg ggcaacaaga 52080 gcaagagttc atctcaaaaa taaataaata aataaataaa taaataaata aataaataaa 52140 aggtgggagg gacacagtaa atattgaatc ataaatgaaa ggctgcatat caaggtgcat 52200 caaaataata aatttcctat ttcattatcg tccagaattt aaagagcatg agcccagaag 52260 tgagaccaca tgttcaaatc ctgacactgc ctactcctct gtgagtcacc tttgacgagt 52320 tccttaaatt cgtcattctc agctttcttg tctgtggagt gtggtaatga cagtgcctac 52380 ctcatacggt tattgtgagc gtcaaattta gtgcttggca aagtgcctaa cacatcatag 52440 gcaagagaga atccaggttc gtagggcctc aagcttttac agtgtctgag ctctctttaa 52500 gaaaatgaat ttcaaactat gaattcaaaa ttagctaatt agaacatttt aaaggtatgt 52560 ctgtagagtg ggaaattata ggcaacaaag gtctgaaaaa taccccagtt atcatcaaat 52620 attattgtta ctagtaattt cctggttcat tataatactt tattttctac acatttttag 52680 atgcatacac tcagatttcc tttttcaaat aataatttta aatattttct ataaagagaa 52740 tagaaagaaa attcattctt tagcaaaggg gattgaaatt tattaatttt ttattgttgc 52800 tggtttagga agtatttatt tacttaattc ataacatatt actggaaatg ccacattctg 52860 acttcctccc tggctttttg cccaggaagt tcattgtttg gaaggaagcc ttagagaagt 52920 tcaggttttg ggtctcactt ggcatggtct cagacaggcc agtgccttgg cagtggctcc 52980 atcctggcag cctcctcttg attccccatc ttcatccttg taagcagggc aggtctttct 53040 gtgagagatg aggggaggaa gtagaaagta gccaagagcc tgggagatag tggtgacaaa 53100 gctttgcttt tacattatta gggtcacagg taaggtccaa aatctccctg aagtccactg 53160 gggttaggtt gtcattgaag tgtagataga tttaaggaaa tgctggcagc ctgcctgctg 53220 acagcatctg ctgcaaactc aggatgattt ttaggaggta gaaggtgagg acaggctagg 53280 accactctcc acacctccac taaaggcctg aggctattac ttcctcaata taggcagtgc 53340 aggtgaggag ctctgaagcc tcaactccat taattccatg gtaaagctgc ttctgacttg 53400 taagtgctca gtaaattagc acttacccga attgttttac tctttcattg gcaaattatt 53460 tttcattaaa accctaggaa ttttgcagtg aatgcctttt taaattttat tttattttta 53520 aaaaattttt gcaataattg tgtttctcct ctttattcca aacaggacag aattctaaat 53580 aacccagcct tttactaaag tgaatttgaa atgagttgtg atagagcaag tttgtttaat 53640 acctcatgat catgttaaga tctaatcata ttgagaaaaa aatgccatat tcatttcatt 53700 cttaaatatc atttctagtg gataccgttc tgtgtcccta gatcctttca gagatgaaga 53760 atttgttatc ccagttgttg ggagtgctgc ttgctacttg tcaagacgac agttctctct 53820 gggacaagtt ctttttgtgc aagatcacag ccactcccta agggcagctt acacccagcg 53880 tttctttaat aggagtggag tagggttgtt tacaaaggcc ctggtctagt gcaccccctg 53940 aaggatcatc ctagcttcag agttccacaa agtctgccta gtgacagaat agtgacctta 54000 cttctccacc ctctgaccaa tttacgtatc ctcccttccc tgacatgtag tgaccctgaa 54060 aactttttcc agtatacctt gtgtacataa accagtctca gagtctcatt cttagagacc 54120 actacctgaa gcattttcaa aattttcaag aggcaataat gtgttaaata acagcttctc 54180 tggggatgag ggaggagatc gctttaaaaa cgtgatccaa ttccagaaat gttcaagttg 54240 aaaaaaatct tacattcaag aaagtgtgct atcagagtat acaagagtgc tataattgct 54300 gtatgttagc tgtgaccaat aacctgaagc aattagaagc ctttcactga agccactatc 54360 tatagaaagg ttaaagattg ttgttctttc cttttctcag tggaattttc tctttatatg 54420 ctcttttggc ttgatctctt tggaactcat ggttttagac cattgcatta tatatctcta 54480 gttctgccat ctcttgagct tctaatttac atattcatct gtctgccaga gtccttttgt 54540 tgatatccca ccagcctccc aaacttaaca tgtacaacac tgagttaact ttccctcaga 54600 tcagtcctgt cttgtttaca gtttcaacta gtggccccac tgtgtccgga attgatgggt 54660 tcttggtctc actgacttca agaatgaagc cgcggaccct cgcggtgagt gttacagctc 54720 ttaaggtggc gcatctggag tttgttcctt ctgatgttcg gatgtgttcg gagtttcttc 54780 cttctggtgg gttcgtggtc tcgctggctc aggagtgaag ctacagacct tcgcggtgag 54840 tgttacagct cttaaggtgg cgcatctgga gttgttcgtt cctcccggtg ggctcatggg 54900 ctcactggct tcaggagtga agctgcagac cttcgcggtg agtgttacag ctcataaaag 54960 cagtgtggac ccaaagagtg agcagtaaca agattaattg caaagagcaa aagaacaaag 55020 cttccacagt gtggaagggg acctgagcag gttgccactg ctggctccgg cagcctgctt 55080 ttattctctt atctggcccc acccacatcc tgctgattgg tagagctgag tggtctgttt 55140 tgacagggcg ctgattggtg cgtttacaat ccctgagcta gagacaaagg ttctccacgt 55200 ccccaccaga ttagctagat gcagtgtcca cacaaaggtt ctccaaggcc ccaccagaat 55260 agctagatac agtgttgatt ggtgcattca caaaccctga gctaggcaca gggtgctgat 55320 tggtgtattt acaaaccttg agctagatac agagtgccga ttggtgtatt tacaatccct 55380 gagctagaca taaaggttct ccaaggcccc accagagtag ctagatagtg tcgattggtg 55440 cattcacaaa cactgagcta gacacagggt gctgattggt gtgtttacaa accttgagct 55500 agatacagag tgccgattgg tgtatttaca atccctgagc tagacataaa gcttctccaa 55560 ggccccacca gactcaggag ctcagctggc ttcacccagt ggatcccaca ctggggctgc 55620 aggtggagct gcctgccagt cctgctccgt gcgcctacac tcctcagccc ttgggtggtc 55680 gatgggactg ggcgccgtgg agcagggggc ggtgctcatc ggggaggctc gggcctcaca 55740 ggagcccatg gagggggtgg gaggctcagg catggcaggc tgcaggtccc gagccctgcc 55800 ctgcaggaag gcagctaagg cccggcgaga aatcgagcac agcgctggtg ggctggcact 55860 gctgggggat ccagtacacc ctctgcagcc gctggccccg gtgctaagtc cctcattgcc 55920 cggggccagc acggccagcc gtctgctccg actgcgtggc ccaccaagcc catgcccaca 55980 cggaactcca gctggcccgc aagcgccgca cgcaggcccg gttcccgctc gcacctctcc 56040 ctccacacct ccctgcaagc tgagggagct ggctctggcc ttggccagcg cagaaagggg 56 100 ctcccacagt gcatcggtgg gctgaagggc tcctcaagtg ccgccaaagt gggagcccag 56160 gcagaggagt cgccgagagc gagcgagggc tgtgaggact gacagcaggc tgtcacctct 56 220 caccactacc ctgacatttc aaataataaa acctattagt catcctagat tctttcttct 56 280 cccttgtcct gcatatccag acagccaaca agacctgata attttcctat gaagtattat 56340 tccaatcagt ttcctctttg caactcctat tgctactttc tcgatttagg ttcttatgcg 56400 gactactaca ataataattg acttgttttc ctaccctaag ccttgcttcc tttaagccct 56460 ctgcatagtg gtccagatac tctacatcaa atacaaattc gatcatgcca ttacatgcat 56520 gaacacaatg aacagcaact tctcctgtca agaaagactc tccatgacat actcctattt 56580 ttttcatttt ccatattacc gccactccct ttatccaact ttatgtgcta gtaaaattga 56640 actgcttgga gtttactggt gtgcttcatg tttcatgcct ctgtgccatt gcccattatt 56700 tccactctgc caggaaagcg cttttcttct gtattagatt aactttaatc attcctttaa 56760 gatgatttat ttttatcatc tctaggatgt ttcttgcatc taaaaatcct ataatactgt 56820 attaatcaat tttcatactg atataaagat actacctgag actgggtaat ttataaacat 56880 aagaggttta gttgacttgc agttctgggg aagcaggcac cttcttcaca aagtggcagg 56940 agcgatagaa catgcaggag aaactgccaa ttttaaacca tcatatctca tgagaattcc 57000 atcactatca tgagaacagc atgggggaaa ttgcccctat gatccaatca cctcacacca 57060 gattcctccc tcaacatgtg gggattacaa ttcaagatga gatttgagtg gggacacaaa 57120 gccaaacatt atcattctgc ccatagcact gcccaaatct catgtccttt tccattttga 57180 aaccaatcac accttcccaa cactccccca atgtgtgaac tcattctgga attaacccaa 57240 aagtccaagt ccaaagtctc atctgagaca agataagtcc cttctgccta tgagcctgta 57300 aaatcaaaaa caagttagtt atttccaaga taaaatgggg gttcaggcat tgggtaaatg 57360 ttcccattcc aaatgggaga atttggacaa aacgaagggg ccacaggttc catgcaagtc 57420 caaaatccaa cagggcaatc attaactctt aaagctccaa aatgatctcc tttgactcca 57480 tgtctcatat cctgggcagg ctaatgcaaa gggtgggctc ccaagacctt gggcatccct 57540 actgggtctg cagcatacag tccctttagc tgctttcatg ggctggcatt cagtacccat 57600 gggttttcca ggatcatatt gcaagctgtc agtggatcta tctttctagg tctggaggtc 57660 agtggctgtc ttctgacagc tccattaggc agtgcctcag tgaagactct gtgtgggggc 57720 tccaacccca caattcccct ccaccctgct ccagcagagg ttctccaggg gctttgcccc 57780 tgcagcagac ttctgtgttg atgtctaggc atttccatac atcctctaaa atctaggcag 57840 atgctcccaa agctcaattc ttgtcttctg tgcacctgca ggccaaacac catgtggaaa 57900 ccaccaaggc ttgggacttg caccctctaa agcacggccc aagctgtgcc ttggtccctt 57960 ttagccacag ctggagctgg agtggctgga acacagggtg ccatgtcccg aggctgcaca 58020 aaggagttgg gccctgggcc tggcccacaa aactgttttt attttcctag gcctccaggc 58080 ctgtaatggg tgtggctgcc ttgaagatct ctgaaatgcc tggagacatt tcctgcattg 58140 tcttggcaat taacttttgg ctgtttgtta cttaggcaaa tttctgcagg cttgaatttc 58200 tccacagaaa gtgggttttt cttttctacc aaatggtcag gctgcagatt ttccaaactt 58260 ttatgctttg tttccccttt aaacataagt tcaatttcaa actatctctg tgagagcaca 58320 taaatacact ttcaggaaaa gccaggtcac ctcttaatgc tttgctgttt agaaatttct 58380 tctgccggat accctaaatc aactctctca agttcaaagt tgcacagatc tttaggtccg 58440 gggaaaaatg ccaccagtgt ctaaagtgac ctttactcca gttcccaaca agttcttcat 58500 ctccatcgaa gatcatctca gcttggactt cattgtccat atcactatca gcattttggt 58560 caaaattata caacaagtct ctaggaagtt ccaaactttt tcacatctgc ctgtcttctt 58620 ctgagcctcc aaactgttcc aacgtctgcc tattacccag ttcaaaagtc acttccacat 58680 tttcaaatta tctttgtagc agtaccccca tctgccaatt atctgtatta gtccattttc 58740 acacttctac aaaggtacta cctgagcgcc actgcacttc agcctcagcc aggggaacag 58800 agcaagactc cgtctcaaaa aaaaaaaaaa aaaaaaaaaa aagatactac ctgtggctgg 58860 gtagtttata aatcaaagag gtttaactga ctcacatttc tacgtggctg gggaggcctc 58920 aggaaactta caatcatggc agaaggcaaa ggggaagcag gcaccctctt cgcaaggtgg 58980 caatagagac aatgcaggag aaactgccac ttttaaacta tcagatcttg tgagagctcc 59040 ctcactatcc cgagaacagc atgagggata ctgccttcat ggtccaatca ccccccactg 59 100 ggtccctccc tcgacacatg ggcattacag ttcgagatga aatttgggtg ggaacacaga 59160 gccaaatcat atcaaatact atgtgttgct tatctataaa ttcctaaagg acaggaacca 59220 tgctcattca cttttgtaac catagtgcct gacacatacg ggatattcag taaatatctg 59280 ctgaacagaa ctgaataatt aacactaata ggtccctaaa tctctttgca cttactgact 59340 tatttcttat ttggtatatt atcaattttt ccatgattgt ttctttaaaa attaatgaat 59400 ttatgattta tttatacagt aaatatttat tgagtggtaa ttgtatgaaa gatacttcta 59460 gatagtggga aaacaagaat aacaaatcag tattaaatga tttagataca catttgttgg 59520 ataaataaga caatttcatt agtcatttga aacaaaaaaa attagtgagc ccatactctg 59580 ttccaggcac tactctggaa ggaagaaaat tctgggagga gaggaaggag gtagcacata 59640 tagactattt ttaagagttt tttgattgta ctgaagacag aaagcttagg cagtagttgg 59700 aagaattggt gaggtcaaga gtttttctat atgagagaaa taattgtata taggtattac 59760 tgatatgaat gatccagcag tgtcagaaaa actggtgatg tgggagacag tgggttcaat 59820 taatggaaca atggtgttga gaaagtttaa aaaggcaagt atttactgca ccaatggatg 59880 gttttacctt aggtagtggc acagtttatc tatggttgta aagggaaaac agagcagatg 59940 gacacagata caaggagatg gaagatgtgg aagtcctctg gtgagtcctg ttttctaggt 60000 gaaataagca agtgatcatc cacacgtgaa cgtggagagt attggtttaa ggaaaaaaga 60060 gaagcagagc gatagtcacc tgagaaaatt actggactat taaatgtaat aaaatagctg 60 120 ggcattatta agggttggtt ctcttgaggt tagagatcat gaattgaaag tgagcttcac 60180 cattcaatat atggtatttc tccaggtaca gtctgatgca ggggtaaggt cacagcaggc 60240 agagaggtga gcttaaccag gtcagatact ttatcaagta atttcaatga aggaaaaaag 60300 aggcagtgct gttgaatctg gttgcaaggg aggtggtaca tgcttatatt taagatcatg 60360 gaaggcctgc aatagaaata actggagaag cccagggctc aattcttaga ccgtcttttt 60420 cctccttcct ttgctaggtg atctcatcca gccttgaggc cctgcaaatc atcaataggc 60480 aggtgtattt caaatttata actccagcct ggacctctat cctgaaatac agcccccttc 60540 tcaacatctt gataaaattg gatatctaag aggcatgaga ctttcaaaaa actctggact 60600 tttcccacag acctggtact tccatggttt cacccatctt aatagatggc agccctgctc 60660 ttttggtatc tcaagccaaa accttcagag ccatccttta ggcttccatt tcaaatgtaa 60720 tatctaatta atcagcaaat cctacaccat cagaatatac ccctctctcc atgtccatta 60780 ctatttactt tggattactg tagcaccttc caatttgtct ccctgtgccc atgcttgtcc 60840 acacaaatag ttcctcaata tggtagccct ggtgtgcttt ttaaaattta acccaattta 60900 tcttctcccc actctcaaca tgctttaaat gggtttccca attaagtccc aggttcttac 60960 ctgatggcag ccacccctat ctaattttaa ctttatactc tttggccaac ttctcccaat 61020 ccttctaagc ctcttctccc tagtctctct tctgctctgc ttctatgata cctttttttt 61080 tttttttttt tttttttttt aagattccac atatgagtga gattatgtag tacttgtctt 61140 tctgtgtctg gcttatttca cttaacatga tgtcctacaa gttcacatgt tctcataaat 61200 gacaacactt cattcttttt atgactaaat agcattccat tgtgaatata tatcacattg 61260 tatttatcca ttcatttagt tggagactta aggttgtttc cacattttgg gtcttggaaa 61320 tagtgctgcg ataaacatgg gatttcagat atctctttga tatatttatt tcattttctt 61380 tggatgtata cccattagtt ggattgctgg atcctatggt agttctgttt ttaattgttt 61440 gaggaacctc tatactgttt tctctaatgg ctgtgctagt taacaatcta accaacagtg 61500 cataagttgt ggcctctctt cacaccctta ctaacacttg ttttgtcttt ttgataatta 61560 gagacttttg cccatttttc aagttgtatt gtttcttcaa atttgagttg tttaaattca 61620 ctatatattc aggatatata cactatatat tcagatgggt agtttgtaaa tattaatatt 61680 ttctcctttt ctgtatgtta tctcttcact ctgttaatag tttcctttgc tgttcaaaag 61740 ctttttcatt tgatataatc tcattttcct atttttgctt ttgctgccta tgattttgaa 61800 gttatttaaa aattatcttt gccaagccaa atgtcatgaa acatttcccc tatattttct 61860 tctggtagtt tcacagtttt aggctttaca tttaagtatt taattcattt tgatttgact 61920 tttgcatata gtctagtctt atttttctgc atgtgatgat gcaattttcc agcaccattt 61980 attgaaagac tgtcttttac ccaatatata ttcttggcac ccttgttgaa tatccgctgt 62040 aggcatgtga acttatttct gggttttcta cgctgttaaa ttggtctatg agtctgtttt 62100 ttatgccagt atcatgctgt tttggttact agagctttgt agcatatttc aggtagtatg 62160 aagaacctcc tacaatattc tttttgctct gaattccttt ggctatttgg ggtgtttagt 62220 agttccacat gaattttaga atttctttta tatttatgtg aaaaatatca ttggtatttt 62280 gatacgtatt ccattaaatc tgtaggttgc tttggatagt atggtcattt taacaatatg 62340 aattcttcta cttcatgaac actggatagc tttccatttt ttcagtgtct tcacattcgt 62400 tcagaaaagg tttataggtc ttagtgtatg gaattttatt actttctatt tttatttcaa 62460 tagatttttg gtaactggtt tttggttaca atgataagtt cattagtaag gtgtaaggtg 62520 gtatctcatt gtggttttaa tttgcatttc cctgatgatt agtgatgttg agcatttttt 62580 catatgtttg ctggccattt gtatatcttc ttttgggaat tatttatttt tgtcctttgc 62640 ccactttttg atgtgattat ttgttttttt cttgctgatt tatttgaggt ccttgtacgt 62700 tatgaatatt agtcttttgt cagtacatag tttgcaaata ttttctccca atttgtgggt 62760 tgtccattta ctctgctgat tatttctctt gctgtgcaga agctttttag tttaatgagg 62820 ttccatttat ttatttttgt ttttgtcgca tttgcttttg gggtcttagt cataaattct 62880 ttgcctaagc caatgtctag aagttttccc aatgttgtct tccagaattt gtatgctttc 62940 agctcttaga tttaagtctt tgatccatct tgagttgatt tttgtataag gcgagagatg 63000 aggatccagt ttcatccctc tatatgtggt ttgtcagttt tcccagcact atttattgag 63060 taggatgttc tttccccaat ttatgttttt gtaaactttg acaaagatca gattgctgta 63120 actatttggc tttatttctg ggttctctag tctgttccat tggtctacat gcctattttc 63180 attccagtac tatgctgttt tggtaactat atcctcgaag tgtgatttga agtcagataa 63240 tgtgatgcct ccagatttgt tctttttgct tagtcttgct tttgctatgt gggttccttt 63300 ttggttccat atgaatttta ggattgtttt ttctagttcc gtgaagaata atgatggtat 63360 tttgatggga attgcattga atgaccagat tgcttttggc aatatggtca ttttcacaat 63420 attgattcta cccatccatg agcatgggac ctgtttccgt ttgtttgtgt catctatatt 63480 tctttcagca gtgttttgta gttttccttg tagaggtctt tcacctcctt agttaggtat 63540 attcctttat ttttttttca gctattataa aaggggtcga atgacaattc aatcgatgtc 63600 ttctttacca atttggatgc cctttatttc tttctcttgt ctgattgctc tggtcaggac 63660 ttccagtact atgttaaata gaaatggtga aagtgagcat ccttgtcttg ttccagttct 63720 cagggggaat gctctcaatg cttttccatt cagtattatg ttgcctgtgg gtttgtcata 63780 tatagctttt attactttga agtaagtcct ttttatgcct attttgttca gggttttaat 63840 tataaaggga tgctggatta tatcaaatgc tttttctgtg tctattgaga tgatcatatg 63900 gtttttgttt tcaactatgt ttatgtgatg tatcatattt attgacttgc atgtgttaaa 63960 ccatccctgc aactctggta tgaaattcac ttgatcatga tgtattatct ttttgatatg 64020 ctgttcaatt tggttagcta gtattttatg aaggattttt gtatctatgt ttaacaggga 64080 tattgttctg tggttttctt tttttgtttt gttctttctg ggttttggta ttagggtgat 64140 actggcgtta tagaatgatt tagggaggat tctctcttta tcttttggaa tagttttagt 64200 aggattggta ccaattcttc tttgaatgtc tgatagaatt cagctgtgaa tccatctggt 64260 cctggacttt tttttttgtt ggcagctttt tttattactg attcaacctg gctgttagtt 64320 attggtctgc tcagggtttc tatttctccc tgatttaatc taggagggtt gtatattttc 64380 aggaatttat caatttcctg taggttttct tgtttgtatg cataaagttg tccatagtag 64440 ccctgaatga tcttttgtat ttctgtggta ttggttgtaa tatctcttgt ttcatttcta 64500 attgagctta attgaatcgt ctctcttctt ggttaatcgt actaatggtc tatccatttt 64560 gtttattttt tcaaagaacc agctttttgt ttcatttatc ttttgtattt ttttctttca 64620 ttttcattta gttctgctcg gatctttgtt attttttttc ttctgctatg tttgggttta 64680 gtttgttcct gtctcttaag ttccttgagg tgtgatatta ggttgtttat ttgtgctttt 64740 tcactttttc atgtaggcat ttaatgctat taactttctt cttagcactg cttttgctgt 64800 atcccagagg ttttgataag ttgtgccact attatcattc ctttcaaagg attttaaaat 64860 ttccatcttg attttattgt taagccaaaa atcattcaag agcagattat taaatttcca 64920 tgtatttata aagtcctgag ggttcctttt ggaattgatt tccagtttta ttctactgtg 64980 gtctgagaag atacttgata tgattttgat tttcttaaat ttaatgagaa tgattttgtg 65040 gtttatcata tggtctatct tgtagaatgc tccatgtgct gatgagaaga atgtatattc 65100 tgtggttgtt gggaagaact ttctgtaaat atctgctaag tccatttgtt ctatggtata 65160 gcttaagtcc attatttctt tgttgacttt ctaacttcat gatcgaccta gcgctgtcag 65220 aggagtattg aagtccccca ctattattat attgctgtat atctcatttc ttaggtttag 65280 taggtattgt tttataaatt tgggagctcc attgttaggt gcatataaat ttaggattgt 65340 agcatctttc tgttggacta atcttttatc agtatataat gtccttcttt gtcttgtttt 65400 actgttgttg ctttaaagtc tgttttgtct gatgtaaaaa tagctactct tgttttttgt 65460 ttccacttgc atggaatatc tttttccacc tgtttccctt aagtttattt gagtccttat 65520 gtgttaggtg catctcttga agacaacaga tatttggtta gtggctgttt tatccattct 65580 gctattctgt atgttttaag tggagccatt tacattcatc attaatattg agatgtgaag 65640 tactgttcta ttcatcatgt tagttgtttc ctagatactt tcttttttat tgtgttattg 65700 ttttatatac cctatgagat ttatttattt attttgagat ggagttaccc tcttgtcgca 65760 caggctgagg tgcaatggtg tggtctcggc tcactgcaat ccccatctcc cgggttcagg 65820 cgattctcct gcctcagtct cctgagtagc tgggattaca agcacccacc accatgcctg 65880 gctaattttt gtatttttag tacagatagg gtgtcaccat gttggccagg ccagtctcga 65940 gctcctgacc tcaggtgatc catctgcctc agcccctcaa agtgctggga ttataggagt 66000 gagccactgt gcctggctga gatttattct ttaaggaggt tctattttgg tgtatattga 66060 acttttgttt taagatttag aacttctttt aggatttctt gtagtgctgg gttggtaggg 66120 gcaaattccc tcagtgtttg tttgtctgaa aaatactttc cctctccttc atttatgaag 66180 cttagttttg ctggataaaa aaagtcttag ctgacaactt ttttgtttaa gaaggtttaa 66240 atatagggcc ccaatccctt ctggcttgca aggtttctga tgagagatct gctgttaatc 66300 tgataggttt gactttatag gttgcctgat gcttttttct cacagttctt aagattcttt 66360 cctttgtctt gactttagat agcttgatga tgatgtgcct tagtgatggt ctttgtgcaa 66420 ttaatttccc aggagttttt aaagcttctt gtatttgcag gtctagatct ctaacaaggc 66480 caagaaagtt ttcctcaatt attccctcac ataagttttt caaactttta tacttctgtt 66540 ctccatccag agcacgattt attcttaggt ttgaccattt tacataatcc catatttctt 66600 ggagactttg ttcattaaag aaaaattgtt tttgtctttg tctgattggg ttaatttgaa 66660 agccttgtct ttgagctctg aaattcattc ttctatttgt tctagtctat tgttgaaact 66720 ttccactgca ttttgtattt ccatggcatg tctttcattt cgagaagttc tgattttttt 66780 aatgatatct atttctctag aaaaattttc atccatattc tggattattt ttgaaatttc 66840 tttaagttgc ttttcacctt ctctggtatc tccttgacta gctgaatagt caaccttctg 66900 aattctttat ctggcatttc agagatttct tggtttggat ccattgctgg gagctaatgt 66960 gatcttttgg gggtgttata gaaccttgtt ttgtcatatt accagaatta ctttcctagt 67020 tccttctcat ttgggtagac tatttcttta aattgttctt gaatttattt ttgatgggac 67080 tgaggttttt tttttttatt ttttccctct taaggatctg actttaatgt ttatatttta 67140 ttttagccta atttaattct tggtgctttt agggtgaaga ctttgtatga gttccttagt 67200 tatagagaga cgttgtgtgt tggtttttcc aaatgttgtt tgtagtagtt atgtacttgt 67260 tgtgtgggca agttcactgt ctcctatgga gttggaatgg cagggatctc ttgaagtttg 67320 tctcattctc ttgtgatgta tactttattt atttaacttt actccagtat tttatttact 67380 gagttgatga ttcaggcttc agtccaatag gagaggtagc ccttggtagt cactggttgt 67440 agctaaggca ggtgggtaca tgtaatcctc agtggtgggc agggatccca gccttgatga 67500 aggtggctaa gggagttctc aattagatgt cctgaggttg taccaggatg aagggtgtga 67560 gctacctcag ttcccctgcc aggccaacag aaaagttatc cacctcatag cctcactcct 67620 gtaccagtgt tctggctatt cagatcatac gggcacctct tttcatgtgt aggaatgttg 67680 atgctccaag tagcggggaa ttgtgacctt gcctcttatg caggcctgaa tatggagagt 67740 actccccctg ttgggctgca atcaccatga atggttccag gaaggctgtc tatagacgca 67800 tatacactgc attcttgtga tagaaactcc agttgtgtcc gcagtgatgt accagggttg 67860 aacaaggatc tcttctccaa gacccttcat gatcacagag gctgcctgcc tgttggggta 67920 gaggtgctga ctttctctat tgtgcccagc tctgcacttg ggtcactgct gaaagaaaca 67980 ttccaccagt ggaaagatct gggactcaag cctgctgttc acactctttt gttccatggg 68040 gtgttctctt gatatggtgt tctctccctt cccctaaaaa tgggacttct tgagagctgt 68 100 actgcagtga ttactattga tcttctgggt ctagccaccc agtagggcta ccagactctg 68160 ggctggtgat ggagaatgtc tgcaaaggat ccagtgatgt gacctttttt caagtctccc 68220 agcaatggat accagcacct gctttgaatg gaggtggcag gggagtgacg tagactgaga 68280 ttccttggtt gtcaataggc ttagtgtgct ggctttcttg aatgctggtt atactaatag 68340 tgaacttgtc atgtggacag actcaggacc cctggatagc cagggtattg caggcagtgg 68400 tgtaagctga gatcccacag ctgttttctc cttcctgaga gcagtgatat tctgctgaga 68460 ggtattgtga tggcctgagt tggctggcct ccagccagga ggtggcgctt gcaagacagc 68520 atcagctgca gtagcagcag tgggatttga gcttgcttta agttgcccag gggaattatt 68580 ctggtttctt aggtgatgga cagggctgta gagctcccaa aagtttatgt tatttgtgtt 68640 catctactag gatgagtgga gaaatatcac caggtgaggg cagggttagg tgggtctgag 68700 ctcagactcc ccttgggtgg ggcctgccgc agccactgta agaggcatgg ggtggtttgc 68760 aggccactag ggtaatgatc cagaggtgag tataactgcc tctgctatgc agaagatttc 68820 acaaagggag tggggagtag ccggcagcag taagcctcat ccagctcctt gcagttggca 68880 aggccaatct cactcctgaa gtgtcctgct atcagcacca ggtttaggtc cacataacat 68940 atgcacagaa ctcagacctg cctgaggcca taagcttccc tgcagaaata gcaagcatga 69000 cttttgggtc acatccctcc tcctctcgtc tgccctcaag gctggtcagc agtgctcttc 69060 ccactcgcca ccaagttctg ttgatgggtg tacatcccta ctcaaaatta tatcacaaat 69 120 tcagctggga gcttctttta ccctgtgacc cctccctgag ctcattggct aactttcttg 69180 agggctcctg tgagatatag tcaggaatgg ctttccttgg ttcatactag agactcagaa 69240 tacctgcaag gcacttcctg cgactgcttc tacttttatt ttcacgaggc tccctaaatt 69300 tgttccagct cttggtagca ttaaggcctt ctcctgtggc ctggattttc agattcctta 69360 gtggggatat gtgctcaaag gtagaatctc cccgtctcat actctgggaa cttttatttt 69420 ttttcctatc tcatggagta ggctgcagcc tatcacttct ttcaaaaggt ctgtggattt 69 480 ttccagtttt cccagtggga gaggcatgct aacactgcct cctatctgct atcttgaaaa 69540 aaaaaaaaaa aaaaggtgta tagatcattc acttccttgg ttaagtttat ttctaggtat 69600 tcgatttttt taattttttg tagccattgt aaataggatc cctttttgat ttccatttta 69660 gctaattcat tattggtgta tagaaatgct ggtgattttt aaatgttgtt tttctgtcct 69720 gcaactttac tgaatttgtt aattttaaaa gttttttgat gaattcttta gggtttttaa 69780 tatataaagt tttatcatct gcaaactggg acaatttgac ttcctccttt ccaatttgga 69840 tgccttttat ttatctggcc taattgtcct gggtaagatt tccagtacta tgttgaatag 69900 aagtcatgga taggcatcct tgtcttgtct ttaatcttag aagaaaagct ttcagcaatt 69960 cccccttcag tatgatgtta gctgttgttt tgtcatatat gacctactga gatacattcc 70020 ttctatgtct aattttttga gagttcttca catgaaggaa tgttgaagtt tttcaaatgc 70080 ttttctgcat attatcatct ggattttgtt tttcttcctg ttaatgtgtt gtaccatatt 70140 tattgattag cttgttatac catgcttacc atgcttgcat ctctgggatg aatcccactt 70 200 aatcatacta aatgattttt ttaatatgct gttggattca gtttgctact gtcttgttaa 70260 gaatcttgtg tctatattcc tcatcaatat tgacctatag ttttatgttt ttgttgtatc 70320 cttgtctggt tttggaatcg gattaatgca ggcattataa aataagttta aaagtattct 70380 cccatcttca attttcttca gtagtttgag gagaattggt gttagtactt tatatatttg 70440 gtggaattca gcagtgaagc tttcaggtcc tggtcttttc tttgatgatg gaagacttat 70500 tagtgataat aataaataat aatttatatt aattgttatt aatttcttta ctcattatca 70560 gtctgttcag attttctatt tcttggtgga ttttctattt taattttgat aggtcatatg 70620 tgtccagaaa tttattcatt tcttccatgt catcaattat tttggtgtat acttattcat 70680 aatagtctct catgatcctt tgtatttttg tagtatcagt tgttatgtct catttttcat 70740 ctcttatttt atttatttga atctctcact tgttttttac cataggtaaa ggtttgttga 70800 tttttatctt tttaaaaagc caaatctttg ttttggtttt tatatgaatt catttttttg 70860 tctgtatttt atttgtttat gttttgaatt gtattatttc tgtccttcct tctaccaatt 70920 ttgggtttag tttgttctta ttatttttag ttccttgagg tgaatcattg gattgtctac 70980 tagaaatctt tcttcttttt tggtgtaggc atttactatt ttaaacttcc ctctcagagt 71040 ttctttcact atgtcgcata ggttttgata tgatgtgttt ccattcttgt ttgtctgagg 71100 gaaatttttt ttcactttta cttccctcat tgacccattg gttatttaag aaaaagttgt 71160 ttaatttcta tgtattttta aattttctga agtttttatt ttagtattga tttctagttt 71220 tattgcatta tggttaggaa agatacttga tatgatctct atcttcttaa aaattttaag 71280 acttgttttg tggcctaaca tgtgatctat cctaaaaaat gttccatatg caggtgagaa 71340 gaatgtgtac tctgtcggat gcagtgttct ataaatgtct gttagggcca tttggtctat 71400 ggtataattt aagtccaatg tttctttgtt gattttttgt ctagatgatc tgttcattgt 71460 tgaaagtggt atattgaagc cctctactat tattgtattg ccctctgttt ctcctgttag 71520 gtctaataat atttgcttta gatatctggg tgctctggtg ttgggtgcat aaatatttat 71580 aattgttaca tcctcttatt gaattgattt ctttattttt atataatgac cttctttacc 71640 tctttttaca gtttttgact tgatgcctat tttatctgat ataagtatgg ctatttctgc 71700 ttgctttttg tttctgtttg catggaatat cttcttctat ccctccactt tcagtctgtg 71760 ttatgcttgt tggcagcata tagttggttc cttttaaaaa ttcattcagc cactctctat 71820 cttttaactg gagaatttag tccacttata ttcaaggtta ttattgatag ataataactt 71880 actcctaaca ttaaaaaaat tactttctgg ttatggttat ttcgtaaatg ttttgttcct 71940 ttcttcctct tttattgttt atctctgtgg tttggtggtt ttatgtgtta ctaagcttgg 72000 tttcttttct ccttcttgtt tgtatatgtg ttatatcttt ctttgtggtt attataagga 72060 taaagggtac tgatggttta tttaaatctg atagcaactt aattttgttc acataaaaat 72120 actccagatg ttttctcttt tctttactat ttatatattt gtttccttaa tttacttctt 72180 tacccattat gtagctgttg taatttttga ccgttttgat ttaaaccttc atactagagg 72240 attaaaagat ttgcataaca ttacagcgct aggatattct gagtttgatt tatgaattta 72300 cctatactgg tgagctttat attttcacac gtttttatta tagtaataat gagcctttca 72360 tttccaattg taccactcct ttaagcattt cttgtaagat cagttaagtg gtgatgaatc 72420 ccgtcagtgt ttgtttgtct gggaatgtct tcatttctcc ttcatttttg aaggatagct 72 480 ttgctgaata tagtgttctt ggctgatttt ttttttttta attttcagca ctttgaatat 72540 gtcatgccat tctctcctag tctgctgaaa aatctgatag tctaatagag atccttttgt 72600 atgtgagtta atacatttgt cttgcagctt ttagaattct ttgtctttga ctgctgacag 72660 tttgataatc atgtgcctca gaaaggatct tttggggttc aatttaatta gggacttttc 72720 agcttcctga atctagatgt ttgtatctct gaatacttgg gaagcctaaa gctattattt 72780 cttaaataca tttcctatgc ctttaaaaaa atttcttctt cctctggcat tcctataatg 72840 tgaatatttg tttgcctaat tgtatcccat gtattctgta ggctttcttc attctctttt 72900 gttcttattt ctgtgttttt cctctgactg ggttatttca aacgcctgtt ttctagttca 72960 gaaattcctt catgaaagat ctagtctatt gttaaaaatt tcacttgtat tttttaaatt 73020 ttcatttatc aaatttttca gctcccagat ttctgtttgg ttcttttaaa attatattta 73080 tatattatgc atttctcatt ccaattattg attgtttgtc tgtgttctat tgtatctcac 73140 tgagcttttt aaagatcata atttttaatt attttttagg catttcataa gtatcatttt 73200 acttggggtc ttctactagg atttattgtg ttcctttggg ggtatcatgt tttcttgctt 73260 ttattatatt tcctttgtct ctgcattgat acttgttcat atagtagaat agtttttgtt 73320 tccaatttta tggagtagct ttttcaggga gagacatttc ctgtagataa atcctagggt 73380 ataagttcag catggtgaat ggctttgatt ctgggtggac tcagtagcac ggtatctgtg 73440 cagtttcttt ggctgtaata ctcatcagtg gtgtctgtga ttgactcagt ggtctacgct 73500 gtgagagctt ttgaggtagt ggcatagttt tcttggggtg ggagcactgg gttggttttc 73560 aggttgggca tgtgggggca cagagagctg acaagctgtc tggctacctc tccagagagt 73620 cagtggctac tgctgtgctg gctgttgggc caggcttgag cttacagaga tgctgccagc 73680 aaggtggctg tgtggctttc ttttcagtga ggcagggcag ccaccaaact agatgagcac 73740 agcaatgcca agcatccctg tggcagtctg ttggagaggc agagctgctg caggactgga 73800 tgtcgggctg catgtgaatg tttgtgggtg cagcaaggcc atgcagctct gcagtggcct 73860 ttcatgggag taggttgcca catgaccact tgccaggctg ggtgcaggca catacatgct 73920 cagagtgctt gctggctgtg aagcacaggt atgtgttggt aacatggcta gtggctgttt 73980 ggaagcttcc ctgctgtgca tatctgcctg ttccctgaga tagtggtgtg ccatgtgagt 74040 ttggacactg gggtcttggt cattatgaca tgcctatgct ctaggtagct gagtttgtgg 74100 tggtgcaagc attcatgtga acatagtgga atgagagcag ggcctcaggg atggagacag 74160 tcagttgcta ttggctccta ctagtgggtc tggttttaag aagccattga gctctatcat 74220 cttaggcctt aagggtggga ggtgttcaag gtgggctcct actgtgaggc agtgcagctg 74280 tacaaacttc tggctaatct ccaaactgga ttcaaaacct ataaggacca ggagactctc 74340 ctgcagcaag gatttctggc atgtgtggta ataattggga ctgctgggga tttagagctt 74400 acctttaccc cataagaaga agtctccctg actctgagct aattctggtg aagatgacat 74460 tgtggcagaa gtggattact tgctctcctc tctatggtgc tattctcgcc ttctgtgctt 74520 cacagggatg ttgccactgc cagggtgctc tccagcatac ttcctcagtc actccagtca 74580 aattatagat gtttattcac tgttttgatc ccttttagtt gggggaacaa gcaccaggta 74640 actatagtca gtcatctttc taacttcact tggacaggta ttttaattat gtgcaatttc 74700 cagattgact tcctctttta ttcaggatta ctttaaatga taattttatg tttataagtg 74760 gatgggattt tttatttgct attcttttat tattaatttg taattcaata ttattcaata 74820 ttgaaaattt tcaatattat tcaatattga aaattttcaa tattattcaa tattgaaaat 74880 tttcaatatt gaaaaatttc aatattattg aaattttcag aattcaatat tcaatttaat 74940 agttcaatac tattaatttt ttggttttgt gatcatataa tgtaacaagt atgaattcag 75000 atttgtgtaa tgtttgagac tttattttag atctaggaca taaacacttt aggtctaggt 75060 catagatgac cttttacaaa tattctgtat gtttattttt aatgcgctat cactgtggtg 75120 taggatgtca tgtatctaat ctgtttaatc aaatgtacat atatactaca taaaaaggtt 75180 gttcattgtg tttttaaaat ttgccctagg cattggtttg ctggagccag ctctaattgg 75240 ctcattaggg ccaatggtta aatagttagg aattttatga gccagctttt aagccattgg 75300 tattttgaag tcagtatagt gaggttttta tacaacaaaa attgggaaat gctacaaaac 75360 aacgattttt tttttgagag ctagtttact ggcatatcaa tgctagcctt actgatttct 75420 gtatatgttt aaatttctaa aaactatgtt aaaatatttg ataacttatt tttcatattc 75480 atttttctct atataatttg aagctatact gttaggtaca taaacattca taactattat 75540 atcttctgag agggttttat ttatttattt tctcaacttt tattttaggt tcagggtgta 75600 tatgtgcagg ttggttacat gggtgaattg tgtgttgctg aggtttggtg tatgaatgat 75660 gccatcaccc aggtagtgag cacagtaatc aatgagtagt ttttcaaccc ttgttctcct 75720 cccaccctcc ccccacacta actcagtgtc cagtttctgt ttaccccaac ttataagtga 75780 gaagatgcag tatttgattt tcaacagaaa atcactaaaa atcattattt tgcctatgat 75840 aatggcttcc agctgcatcc aagctgctgc aaaggatatg atttccttct ttttatgact 75900 gcatagtatt ccaaggtgta tatataccac attttaaaaa attcagtcta ctgttaatgt 75960 ggaattactg cagggcctca gcaatggaga cagttattgg cccctggggc aggataccct 76020 ctattagtgg gtctggtatc aagaaaccat tgagctgtat aatcttaggt cttaggggtg 76080 ggaggtgttc aagagccagt gttaatggac attctcagag agttttaact tttatccatg 76140 tgcattatcc attatattgt ttagtacttc tcacttgagt tctacttttt ctctattctt 76200 gttactacag tcatatttac ttggcatatc ttttttttgt aatttttaag catttcgtat 76260 cattttattt ttgtttcatt tcttataaac tcattttaaa atttatctct gtgtctactt 76320 tttaactaat gttagtatcc atctcttagt aaggaaatat agtttattta catctttgct 76380 tgcatgagtg aatgattact ggtatgttta gacttactac tgccacattg ttttttctgt 76440 atatttttac tgcactttct tttttttctt ctctctttct gcctgtcccc actacctcta 76500 ctactattac aaccttctgt tagattcttt gagttttgtt tgttcatttt attttatttt 76560 ggtttggaaa ttatacatgt ctggcttggg tttttttttc atagttaaat tttctcttga 76620 ctttttgcaa ttaattagca taagtgtatt tcaaattatc acaaataaaa taatcatcag 76680 agtatgagtt tacttcctcc tcttcctttt cctacaccat ttcccccagg gagagtatcc 76740 atgattttcc ttgcacattg ctacttgtaa aagtacatgg ctaaatcact agtttcctag 76800 cattgcgact gatgatacag tgactgaaaa ggaggatgag ataatttttt ataattacaa 76860 atattttcta tatcatggtt gtggtagtaa tttcatgagt gtatagtgtc atcagaactg 76920 tatactgtat gcttaaaaca agtgtatatt ttattatctt aaaattgtat ctcaataaag 76980 tttatttaaa agtatggtac agaatgtcag acagagcaag aaataattag caggatgtag 77040 agaaagccca ttgcaagtta catcttatga aattatactg gcatggtttt tccagaaata 77100 cttagcagtt tggaacttga aattggcgag atgaatgtag cagaaagatg aaatgtgggg 77160 taaaattagt gtgctattta ggatagtatt tattacagtg actggctggg ccaccttctg 77220 gttagggaag ctagtgcagc taagatgctg atagttgtga gaataaggac aatctgttta 77280 taatataaac aaattaaaaa tcaaatccag taattatgtc atatgtgctt tttatatgct 77340 tagcatgctt ataggtgttg taggtacagt aactagtaag atacacaagg tttctgacca 77400 caagaatttc acatttttgc aaaatgaatg atgtcaaaaa tgtactttaa tgaattgcca 77460 ttagttattt agactcaaat ataaatgtta ctaattctgc atacttatat cccacatctt 77520 ttttgaataa ctttttaatt tatctggaat gcatttttta cttaattatt tcatagacat 77580 tctgtgggtc ttatattttc taagatgtgc tatgaaagaa tgtttttgcc ctccttttca 77640 aatactaata gctttctagg ttcaaactca ttctcactta gaaccttgaa gatgaattct 77700 cttgtcttgt aagtaccatg atcagattca ttctgatttt tgtccttaaa tagctttcta 77760 ttagcatgtg tttcattatc ctggaattag gaaattcaat ttaaaattgc tgtgtttttc 77820 aattatccct tttatgccct gtcaatgtga aagcttgtgt tctacttcag ctctgtaaac 77880 ttttctttta tgactgtttt gattgtttca ttctgtctgt tgtctctttg ctgtctgttt 77940 atggaactcc ttttggatac tatgttggaa tttctacacc tatctatctg ataatttcca 78000 actttttctt cttctgtact atggtttggg ataatttctt caccttagtg agtgtttgaa 78060 tgaagtgctt ttgaaaactg actagaggaa cagatatcat tgattgtttc cggacagtag 78120 gttaaatata tttttgctgc cctgaggaag ctgtccattg ttcctgccct ggagaattat 78180 actagctggg atttttcaaa aacatttata ccaaacattt tcaaaaacat ttgcaccaag 78240 tgacaatagc ccatgaacat agtgctacag aaaactatga tcagagagtg gtaagagatc 78300 taccatatat cccctccagc tacataaatg aatatccctt gtttacctta aacaataagc 78360 tacctgaggt gtttctatat ttattttata tatttctata aataaatata ttgtttaaat 78420 atatattaaa tattgtttaa atatatattt ctatatataa atatattgtt taaatatagt 78480 gtttctatat ttacccacta tctgtttaat taatgctttt tgatagtgat tgaaactaag 78540 atgtccaaat cttactgcta cctctgtccc tggcagctca aaaaatattt atatagatta 78600 ttgtttgctt tggaaacatt tcaatataat taaccaattt gtcttatttt aagcattcca 78660 aagaacttaa aggtgtttca ttaaaaaaga tacatatatt tatatacctt tgtatgaagt 78720 agttacctaa tcattttttt ttgttttgat aagctgaaat ttataggcct tataaacata 78780 ttgtgtagcc ttataagaga attaatacca acctcaggtc ttatatttgt atgaatgaat 78840 ttttaaaaat cattatagta acccaagtta ttatagtttg tattggggct tatttagcct 78900 tgatattgaa tacagattaa gtttgaggac atttcttatt tgaaaatctc atattggtgt 78 960 caatttaaaa cagtttgaaa gcaattgctg aaaatgtctt agtgcgatta atgataggaa 79020 gattggagat gtttaaagat atcactttct cagctgtact taaattagga ctgaagagat 79080 ctgtagcggt caaaagctgg caaaccaact gtttatttat caaaaccgac tagttattta 79140 tcaaagcagg tggttggtga ttcaaatcca tcaaccacct gtttttataa ataaagttgt 79200 attggaacac agccataccc atttgtttat gtgttgtcta tagctgctct tacactacag 79260 tggatgagtt aatcactggc aacaaagtct acaagacttg caaagcataa aacattatct 79320 ggccctctac acaaagtgtt aaccagttgc tgctttaaac aacggtgaca gttttgtgga 79380 gtcttactga ccactacttt catactgtta ggaaaatggc tcacagtcat ctgaaaacac 79440 actatacttg gttgcatatg gccttgcttg ggttctgcct tctgcctcaa tgctgttttt 79500 ctcctttcac tcatgcctag aatatatcca accttcaagg tatgattcac gtaacactac 79560 ctttatgaag tcctatcata aagaaatgaa gtaaaatttc ctggacaaat ctcccaatcg 79620 ctcccttaca tatagtttga aatcccttgc ccctctcttc cttgtacttg cttggtatac 79680 acataacatg tttaaatcca gttctgtacc tattccaggc tttctttccc tttcagctta 79740 ctgtggctgg agaaaacaca taccacccta atgactaatc tcactccaca accattcaca 79800 acattgatcc ttcaaaaggt caggctctca tactattctc ccagttacct aaatggctat 79860 tttgtgtgtc ctcttccctc tcctcctcct cctgtcccca tcctcctcct gtccccatcc 79920 tcctccaatc ttcactttct gctcatgtct ttttatttta attcactgag aaaatggaag 79980 taatagtaga aaacttacac ctacctacca gccccatccc ccttataact cagcattcct 80040 tcccgtgaca gtagatgact cagccctcgc cccctcttgg aggtgagcta acatcctcat 80 100 cttcacttgc ttattagaga acattactct ggcacttccc accccctcca aaatccttaa 80160 ttccccctct ctaataagtc agtcccatca gcatgcaaac atgttgttat ttccaccact 80220 taccacctgc aacaaccccc tttccttcct cccttttgga gcaggattct taaaaacatt 80280 gcctgcacta gcatcttaaa aaccttttct tgctactttc ttgtcagcca tcccaggcag 80340 gcttctgcct ccatctaacc cctaaaagtg ctattgtcag agtcatctga catgacctcc 80 400 gggttgccaa gcccactggt cagtgctgaa actgtgtctt gcttggcctg tcagtgcatt 80460 tgatgtgtcc atcattccct tttgctggat atacttccct tgactgactt ctagaatatg 80520 atactcttca ttttcccctt attcagtagt cattcctttt gatttttctt tcctggtcct 80580 tcttttataa tccttgacct aattaatact gaagtatccc atgctcaggt tttggtcctt 80640 ttctctacct acatcactcc ctcatgagca ctctgatgag ttgcatatct atttccagtt 80700 cagacacatt cctgaactcg agatttatgt atccaatggc atatttgata tttccttgtg 80760 catatctaat gagcctttca aactcagcat gtccagaact gaaactcctg acctgtcccc 80820 tctcctcaag gcatcagctg tgtccaaggc ttgctttttt cagttgatgt cagctccatc 80880 ttctcaggtg ttcagaggaa aagactcaga gtcattctca ttctcttttt cctcctgtac 80940 cctgcaatct gtctggaaat tatattagtc ctatcataaa aaatgattcc agactctaac 81000 cacatgtatc tatctccact gctaccccaa gcagattcag gtcctcctct cctccacctt 81060 tctgacctca tctcagacta ctcttgccct tgctcattcc actggcttct tttctgtccc 81 120 ttagactcat agccacacag ttgcctctgc cctagctatt ccctctgctt caaatagtct 81180 tcactgagat accaccgggg ctcacaccct ttctatcttt accctgacta cccaaaattt 81240 caacctcacc cttcttacct tgtttccttt ttcctagata atttttcact ctctaataaa 81300 atacataatt ataataactt atttgtgcat tttctatatg gttcatcttt ctttctgaaa 81360 tcaagattgt gttctgccat cattgatgta ttccaaggtc ataaaaaatg cctagcccat 81420 aataaatatt cagtgaatat ttactaaatg aatcaaggaa agtgatgttg tcttatagtc 81480 tctatattct attcagtctc ggcttgtctt ttctagtggt ttgaatgaat ggatgatgca 81540 tttaacccag aaaaccaata cttgtaaatc ttttattctt gtatgtttaa ttaattttaa 81600 atgtgtgatt agacaagtta ataagcagaa caaaacatca agtatgttag atcatctttc 81660 ctcactggga agaactataa tattggtata gaatgtaaga tgtttacagt tattttcaca 81720 agcaatgatt tgcttgggaa atagcaatgg tgtgagcccc gacagtatct gggtgaagaa 81780 tatttgaagc agaaggaaaa tctagagcag aggcaactgt gtgactacga gtctaaggga 81840 cagttttcac tccagaatga aaactataga gatgcctgcc tgcgttaact tgctcacctc 81900 actatgtatt ttccctgggc agtaccatcc tgctaggatt tcagtgctgc catgagtcag 81960 cagtcacctt gcagtgagat ccaatcacaa gattccaaac aattcctacc ctgtgagctc 82020 cttgaggaca gggctgggat ttagtctctt ttatttcaca ggcccagagt tctaggatgg 82080 cagaatggtt tcgggtgcca ggccccacct ggttgctctc catgggcata ctgcccaggg 82140 ctgccttggg acttggctcc ctacattttg gtgcagcagt cttcagccac cccaactatg 82200 gcttaagtgg ccccagctgc agcttgaccc actgcttcac aagatacagg tggtaagcct 82260 tggtgaaatt catgtagtgt taagcctgca gtctcacaga atacaagagc tgagggggca 82320 tggcttcctc cactttgatt tcaaaagatg atgctgactt atttggggca caggcagaga 82380 ctttgcacag gggtagaacc accacagaga gcccccaaca gggcggtgcc tcatggagcc 82440 aaggaaatgg ggttgccacc aaaatcctag aattatggtt aacagtgtag agctgcagtc 82500 tggaaaagct tcaggcatga gactccaacc catgacagct gaagaatagg ctgagcctag 82560 gaaagctatg ggagctgagc tgcctgaggc cttaggggtc caacccccac ccagtgtgcc 82620 caggatgcag aatatggagt gaaaggaaat tattctccaa ctttaagatt taatattgtt 82680 ctccctgttg ggttttggac ttgtttcaga tgagttatcc ctcttttctt gcctatttcc 82740 ccattttaga atgggaatgt ctcttctata ccttcgcaca ttgtattttg gaagcagata 82800 gcttattttg atttcacagg ctcgtggttg cagaacattt gccttcagga tgaagtcaca 82860 cccatatttg atttagataa gtattaccta gataactcga ttaactagat ttagataagt 82920 attaactaac agtattaaga gttaatactg gagtgacttt tgagcctact aggatggaat 82980 gaatatattt tgcattgtat gaaggatgtg aatttggcag agaggggagg gtaggtgcag 83040 aatgctatgg tttgaatgcc tccaccaaaa ctcatgttga catttaattg tgaatgtgac 83100 agttttaaga agtgggatct ttaacaggta attaggtatc tctttaggca ctttcattag 83160 acagggccct catgaatgga ttaatgcagt tatggaggga gtgagtttgt tattgcatga 83220 gtgggctcct tattaaaggg tgattttggc tcagtttcct ctctgtctca tgtgcttgct 83280 ttcacttcta cccttctgcc acaggatgac ccttagcaga tgtcagtgcc atgcacttgt 83340 acttcccagc ctccagaact gtgagccaaa tgagcttgtg tcccttgtaa attaagtttg 83400 tggtgttctc ttatagcagc agaaaatgga ttcaggcagc aagcagagct ataaaatact 83460 tctttttttt tttttttgag atggagtttc acttttgttg cccaggctgt agtgcaaagg 83520 catgatctcg gctcaccaca acctctttct cccgggttca agcaattgtc tggcctcagc 83580 ctcccgagta gctgggatta caggcatgca ctatcacacc cggctgattt tgtatttttt 83640 tgtaaagacg gggtttcacc atgttggtca ggctgatctc aaactcccga catcaggtga 83700 tcctcctgcc tcggcctccc aaactgctgg gattacaggc acgagccacc atgccctgcc 83760 aatacttctt ttcatctaca ctggcttcca ttatcatgat tttgttagat acttttgagg 83820 acattttcta ttgcattcct ccatgtttgg caaccacact ctatggtgga gtcttctggc 83880 taagcactta gtcttctttt acttatacca gcaggatgta gttgagcagg gtagtgaaaa 83940 tccattgtga cattgtaaag cctcactgct ataagaggcc aggaatgaaa ttttcattgt 84000 ttacaaaagt agcttcgtaa ggcctaatta agaattatat cacccatggc attgctcaat 84060 cccaagtcaa tatttttttt ttttgaaaaa tgtggattca aggataccac aactaaaagg 84120 gatgtaggga atttaagagg ctttggatga aatgatgaag attacggtaa agttttataa 84180 aaaatttgaa gcactgagat aatatagaca aagggaataa ttataaaatg agagtgataa 84240 ggaaggtttg ctttaaataa accaatcaat gagtgattta gatcatatgt aacaggaata 84300 ttagatttga tatgttcttg tggacaaaaa ggtatttcta ttggatacac ctttgtatct 84360 tgcttaatac ctaaataagt gttagctttt tctttctatg agttatagac aatacatttt 84420 aaagcagcca ttttttatat ttcagaaaca tctgaaaagt ataataattt aattctttgt 84 480 gtttatggaa cttaattcag tgaatattta tgtcatgggt tgtggcagat tttggttttt 84540 tgtggaagag gtcaccatgt atatactatt ttgaagggca gaaaatagtt gtttctattt 84600 ttgtatattt tggttataaa ctgcttttaa tggcatggag ttgaatttag ggatgtttaa 84660 tcttcatatt cttcacttga tttgagtgag aaaaaataaa attttcttgg ttataaaatt 84720 ctcaaatgat taacaatggt ttctttaaaa gactgaagat acatattcag tggctgtaga 84780 aatggctact gctattgtaa actggattaa caatcagtac ttctacaaat ctaagtattt 84840 gtttgttttt actttttact agtatgactc tggaattttt caattcaggg aaataataga 84900 atagaaaagc tttgctaagg cattgtaatc agacagaact ggcctcaaat cctggctgtc 84960 atgtacttgc tatgggccta gagtagctta cctaaatgct actaaccttc ctccatacca 85020 ttattgtaaa gattaaaggt gatgcatctg ttaagtaact aatagagtgc ttattaaaag 85080 gtaggtgttc aataagtatt aattccctcc ctttcttttt cttactagtg cacttgtgtt 85140 tttaatggat catactttac cctagattgt attgtaggag gcattgtgga tggatggctg 85200 ctggaaaccc cttgccatag ccagctcttc ttcaatactt aaggatttac cgtggctttg 85260 agtaatgaga atttcggtaa gaaaaaaaat agatgaaaat atcctatgga atttccctta 85320 aaggtcgtct gaatctcaga gtctttgcaa taagttacat ggttattctc caaagatctt 85380 gagatatcac agatgtctgt tcacatttgg attgttctta ttttgaaaat aaataggttt 85440 ttaaaaactc tattgaccat cttgataggc tcttcttgtc attataaatg tgttatttca 85500 cttatcccac tcttttgtgt tctctaaatg tctttgtact cactaaattg tgtagtctta 85560 gagggcaggg tgtgggtatc attcatcttt ttgaaaggaa gcatgaacaa ttagtggtta 85620 aggtggcagt taacactgct ttctgaaact ttaaaagctt cgacaacaca aaagaggcag 85680 gtgaaagtac caaaactctg gtaagttgta ggataaatga ttttcctaaa tcaccaagaa 85740 agattattag tcactctcaa gatggaatac agtatctgta attggttcca ttttatccaa 85800 tttttcacca aataatatta atataattca ttttcataat ttacatgaag ttaatataac 85860 tattgcatct ctgttagtaa tgcatgttat taacctgtgc tgtataaaat attttgacct 85920 attaaaaaag aaagaaggat gaggaggagg tcaaagataa tataaaaact ttaagtggtg 85980 acattggtat gtatgtaaat taacaaatat gtcaactttg ttaaattatc tttgacatgc 86040 atctgcagaa atatctgtta aggaactttg tgcataagag acagagttgg aggtgggaga 86100 gaaagtgaaa aaagataaat tatagtttta ttatttttga taaggggcat taattatgaa 86160 attatgacaa acttacatct ttagacctaa aaatatgttg acatgttatt ttaaacctca 86220 ctttttaact aaaaaattta accatttgtc tatgattata tttttcagaa accacatttg 86280 agaagtattt ccatccagtg ctacttgtgt ttacttctaa acagtcattt tctaactgaa 86340 gctggcattc atgtcttcat tttggggtaa ttttatcttt aggcataaat aacattatgt 86400 tcatggtcat gatgattgtc cttggatata ttttccatta aattagttta cgttcagttt 86460 gcttatatct ctaggtactc agtatctggg ggatagaagg cagactacag agatttagag 86520 aggtttggta aacacctaac tgagcatttt tctacattgt ggtaatagtc caaacaagca 86580 ttatagccct caaataaatt gggccttttg atatttaact gaacttggaa gtataccact 86640 tattgataga ctacatattt ttcatggcaa ttaaaagtag atatttagaa tttgcttatg 86700 ttactttttt atctgtccct gacactgact cctcctatcc cttgatcttt atcctagttc 86760 agtttaaaac atttctttct ttatttaagc cttttgttgt ttccttctaa taaaattcat 86820 atgtttccat gactattaac ttttttcttc tctcttagat gcagctaata tacccagttg 86880 gcccaaagca cctaacctat agttatataa tctgactctc agttcagttt tactctacta 86940 atgccttcat ggtattggga accatagatt tgtgcaggta attcttcatc ataagacaga 87000 ttagtgttga ctatcatgct tctgtgtaag atccatcagg aagtgagtga ttatttttcc 87060 tcaagctcta agcatcattc caatgttact tggtcagtat aagtgtattt tgtctggata 87120 ttaaatttta atttttatga aatttgtgaa agtgagtaag tatatgatca ttcagcctac 87180 actggaatga agtctgtact gtttatggag aaagctgctg caagcatagt aagagtgtga 87240 gcaacatgaa aggggcatgt ttaatgtagt gtaaaaaaaa tttactatca aaatcattac 87300 atgaaaatga ttattactca ttttaagaat tttacctatt tattagactt caaagtctct 87 360 gtaaatctct acttaaagga aactagtaat tcattcaaaa tcatttattg agcattgcca 87420 cacatctggc actgcttcta tttcattgtg ataaattgag ccagtttatt tataaagtat 87 480 gcctacagtt aggcttttct ctaatccggg ttggtgaagc ctcaagttat cataaaatgc 87540 caaaattgta ctatatgctc aatgtttatg ttcagttaca aggctgttga atgcacagaa 87600 gcaaggataa cactgatttt ttcactggtc agaataaaaa ttattgattg ctcttttgct 87660 tatagtattc atcaagatga ataggctcct tcaaaatgct ttgctctatg gtttttcttt 87720 cagctgtttc agtgcagggc ttcctaaaac agaagccaac tgggtgaatg taataagtga 87780 tttgaaaaaa attgaagatc ttattcaagt gagtactcat ttttccataa aatgcctggt 87840 ataactgcta tggagtttgt ctctctctgt gtttttctgt ctgtcttaag gaaaccacac 87900 ttgaaacaat aatatttttg tagaaattta tgatcttata aagctcaaaa agaagtcttt 87960 tccttactgt tttagaatct aattatcttt aaaacgtaat ttataagcgg cccagacgga 88020 tgagttctag tctgcacagg tgaggtattc tcttcctcta tctggagaag ccagatatct 88080 tggtggcacc tgcagggaga atggcttgac ccagggaaca gctgttgttg cttgtggact 88140 aaaggccctc ttctcccacc tcaagaaaac aggtggcctg acctaaggaa gtgatttttg 88200 ccatagcagg taataccagc ttcagtggaa gtctcgatgg caccaagcat accaaaatag 88260 caccacaaag ttctgaaaac taaattgtca ttggaatcac agcattaaaa aataggttag 88320 aacttgcatg ctaaatccaa acaacctgac tgcatgttaa aatagaagat ttaaatagga 88380 ccagagtgtc ctaacataat agacatgaag ccaagataca attggaaatc acccattgta 88440 acaagaaata ggaaagttac aacttaaatg agaaaatata gtcaacagat tacaaaattg 88500 aagtggatca gatgttggaa ttctctgaca tagatttgaa agcagacatt ataaaaagtg 88560 cttgaaaaag caattacaaa tgcccttgaa gcaaatgaaa aactaaatgc cttaacaaag 88620 aaatagaaga tatgaaagga accatataga aattatataa ctgaaaatat aactgaaata 88680 taaagcaaac tgtaaaggct cattagcaga atgaatgaca gaggaatgat agttaatttg 88740 acaacagaat actagagaga cagttgactt aagaaaaaaa aagcatcact gatcttgacg 88800 acagtaacaa aagattcagc attcctatgg ttagtatcac aggagagtat gagaatacac 88860 caagtaaaag agagagattg gttgaattca taacaaaaca ccacccatct atactatcta 88920 taagcagttc acttctagta caatgacata cataggttga aatttaaaaa gacagaaaaa 88980 gatatagcat actataatgg gttgaatagt gtccccccaa atttcacatc ctcttggaac 89040 cttaaaatgt gatcttattt tgaaataata tctttgcaaa tataattaat gaaattaaga 89100 tgagatcata ctagagtagg gggggcccta aatccaatga caagtgtctt tattagaaaa 89160 ggaaaggaca cagagtaact cactgggaag aaagccatgt gaagacagag gaagagatca 89220 gagtgatgcg gctataagcc aaggaaggcc agagattgcc aggatccacc agaaccttgg 89280 ggaagtcaag aaagcattct tctctagacc cctcagaagg aacatggccc taccaacacc 89340 ttgactttgg acttccagtc tccagaactg taaatgaata gatttctgtt gttttaaacc 89400 acccaccttg tggtagttta ttacagcagc tctagactga tacacatgca tacattaatt 89460 ataaaaagca gaagtggcta tattaacatc aaagttaatg ttattaacat aaagtagact 89520 tcacagaaaa taaagttgct ataatccaga gggatattac aaaatgataa aaaataaatc 89580 tgtgaggaaa acataatgtt cttaaatgta tacacatcaa acaacgaaga ctcaaaattt 89640 gtgaaacaaa aaataatagg gataaaagag aaatagacaa atccacaatt attcttgagg 89700 acttcactgc ctcctcctca gtaactaatg gaagtactag acagaaaatc agaaagggca 89760 taaaaataca atataaccaa ccaacaggat ctaatttaat acatagagca ctacacccaa 89820 caacagcaga atatactttc ttttatcaag actaaatata tactgagcca taaaagaaac 89880 tttaaaaatt ttttcagaac ttaaatcatg cagaatttgt tctcaaacca caatggaatc 89940 aaaccagaaa tcaagaaatt aaatacaacg ggcaaatctc caaatgcttg gaaattaaat 90000 actatacctc tatataatcc atgggtcaaa gagtaaatct caaaggatat tcataataca 90060 tagaatgaaa aaaaatgaaa tacagcatat caagatatat gtgatgtggc taaagcaata 90120 ctgacaggac attttttaaa atcaaatgaa tacattagaa aaaaaggaga attatcaaat 90180 caaaaatcta agttactgct tcaagaaact agaagaagta gagcaaaaca aattcaatac 90240 aagcagaaca aagaaaatat ttaaatataa gagccaaaat taatgaagtt gaaatcaaga 90300 aaacaataga aaaaattaat gaaacataaa actggttatt tggaaaatca atacaattga 90360 tctttaattg acagaaataa gagagaagac acaaatctca ggaatgaaat ttggggctgt 90420 cactacagaa tctgcagtca ttagaaggat aataagaaaa actatgaaca attttatgct 90 480 cacaaatctg ataacttaga ggaaatggac caattcttca gaaaccacaa actaccaaaa 90540 cataatgaag atgaaataga caatccaaac actcatataa tcactaaata cattgaattc 90600 atcatacaca agctcccaaa aagggacctt caagcctgct ggttacactg aatcattcta 90660 tcaaatattt acagaagcat taacaacaat tgtacagtct cttctagaaa atagaagagg 90720 aaggaacatt ttccagttga ttttaggagg ccagtattac tgtgatgcta aaaccaaata 90780 aagatattac taaaaaagac aactacaaaa atatctccca ttaacttaga tattaaaatg 90840 ctctataaaa tattaacaaa tcaaattcag caatgtataa aaagaattat acaccatgac 90900 cgagtgagat ttatttcagg tacacaagtc ttgctcaatt atttgaaagt caagcaatgt 90960 aatctaccct atcaacaggc taaagaagaa aatcatatgt agaaaaggta tctgataaaa 91020 tgcaacacct ctcatggcct gtgtccctcc ctatgcctcc tggatttata tgttgaagta 91080 ctaattgcca gtatatcaaa atgtgactgt attttgagat agggtcagtt aaaatgaggt 91140 catgagggca ggtcctactg caatatgact ggtatcttta gaaaaacaga taagggtata 91200 gatacacaga gatagaaaac catgtgaaga cactgggaga aaatggccac ctataagcca 91260 aggagggagg actccaaaga aaccaacact gctggtaccc tgatttcaga cctctagcct 91320 cctgaattgt gagaaaataa agttctatta tttaagtcac caagtctgtg gtacttttta 91380 tagcagccct agcagactga tataaacacc tattgaagat aaaatttctt agaaagtagg 91440 tgtggagaac tgcctcaact tggtacaaaa catgttcaaa aagtctagag ctaccatact 91500 taatggtgag tacaagactg aatgtttttc tgctaggact ggaaactaag cagggatatc 91560 ttctctcacc acttttactc aacataattg tctgagtttt ctgttgctta taacagagta 91620 cttgaaatgc ataatttata aagaaaggaa tttatgtctt atggttatga gactgaaaag 91680 ttcaaggttg aggagccaca tctggtgagg gccttcctgc tggtgggggc tctctggagt 91740 ccagaggtga caaaaagcat cacatggcga ggggctgaat gtactcatgt gctagctcag 91800 gtctctcttc ctcttcttat aaaactaccg gttccctttc cgtaataaca cattgatact 91860 ctaatccatt catgagggca gagccattgt gatccaatta cctcttaaag ccccacctct 91920 caatactgcc acattgggga ttaagtttca atctgaattt tggaggggac attcaaacca 91980 cagcaataat attggaagtt tagccaaatt atgaagcagg agagaaaaaa tgtatgagaa 92040 aaaaataaat aaaaggcaca cagattggaa aggaaaatta tacatcaccc caagtttttt 92100 ggagagaaag tagtaaaaat gggtatattt tgtttagaaa gtaatgcaaa ttaaaaccac 92160 aatgacatat tattacatgc agctataagc gtatacagaa taaaaaatag tgatgatgct 92220 aagtaaatat aaatgaacaa gggtgataat gctggcaagg atgtggagaa actggatcat 92280 tcatacattg ctggtgatga tgtaaaatga tacaggcact gtataaaata gtttggcagc 92340 atcctatgaa acaaaacatg catttgcctt acaatccaac aattgtgctc ttagacattc 92400 atccttgata aatgaaaact acgtgcacac aaaaatatgt acatgaatgt tcacagagat 92460 tttttcatga ctgccaaaaa ctggaaacag atgtgcttca gttggtaaaa tctgtgatat 92520 atcatgtcag ggaatactac agagcaataa aaaggaacaa actagtgata cccacaagtt 92580 aggtggatct ccagggtatt atgatgagtg aaaaaagcca gtcttcataa agaaactgtg 92640 gtacatttat aaaatagaat attatccagt gataaaaaga aataagctat caagccatga 92700 aaagaaatgg agaaaccttc aaagtcatat tgttgagtga aagatgggga cgccaatctg 92760 aaaaggcttc acactgtatg atcccaacta tgacattttg aaaaaggcaa agctatggag 92820 acaataaaaa gatcagtggt tgctaggggt ttggagggaa ggagaaatgg ctaggcagag 92880 cacaggctac ttttaaagca gtgaaaatat taatatatga tgatgggtgg cattatacat 92940 ttgtcaaaac ctgtaaaaca tacaacacaa ataatgagcc ctaatattct gtacacttaa 93000 gatcataata gtgattcacc aactataaca aacacaccac actaatgcaa gatattaaca 93060 atagggaaac tggggtggag aacaaagggg tgtataagtc tctgtacttt ctgctagttt 93120 ttctgtaaac ctaaatggct cataaaaatg tctatcaatt cttttaaaag ctaatttcag 93180 aaggttacat atgatatgag tccgtttaca taatattctt gaaatgacaa aattataggc 93240 atggagagca gagaagtggt tgccagaatt tagagagatt gggtgatgat gaggtatgta 93300 tggggagaaa ggtgactttg gttatgaaag aatagcagca gaatccttgt ggtgatgaaa 93360 ctattttgtg tcttaactgt ggtggtggtg atgtgaatct atgcaaatga taaaattgca 93420 taaaacttca cacatatgca agcacacaca ctgtggcaca taaaacttgt gaaatctgaa 93480 caaggtgagt gcattgtatt aatgtcaatt ttctgattgt aatactgtac tgtggttatg 93540 gaagacatta ccactgggag aagcttgttg aagggtacac aggatctctc tgtatttact 93600 cttacagttt catatgaatt ttaaattatc tcaaaataaa aaaagaatta aaaacaattt 93660 aatgttttca gtaggttgtt ctcataatat ttaaagcagg gcatagtttt taatattgtc 93720 taattttgtt tcctttcagt ctatgcatat tgatgctact ttatatacgg aaagtgatgt 93780 tcacgtgagt atactttttt tcaaaattgc tatttgtctt gttattaaag tttttaaaag 93840 tatatttttg taacaactaa aatatggtag ttttaaaatc taacttttgg tgtgtttcta 93900 taatatatgg tcaatgagtt ttattagtga ttattgagat gggacttggg gacagatagc 93960 tacatcaatg ctatttttgt aatggtgcca aatcaaaata ttaaaagagt cttttccatc 94020 aatgtcaaac aagatacatt atacgttttt gtgatgcagt tgcatcatgg tgcaattaga 94080 tgcaaaaatc agattaactg tttcaattta agagtagcca atatatcagt tatttccaat 94140 gctgtaatgt atcaccatct gcaatcatat tttccataaa ttgcttaaaa tcattcatgt 94200 tctgcatttt acatgattct ctacatttgt cagaaaaaga ttctaattat tctctatgtg 94260 agaaaggaaa cataacccat gaaacttatt agaactgaaa aatggtcttg aacttgtttc 94320 attaataccc tgacatttcc taaggaatgt aggatttgtc atgttggtgg tggttcttcc 94380 cttcagtatt gttactaaaa ccaagaaatg aatccccaac ttcacgtcag ggaaaacaaa 94440 ctctatgtcc tctctccatt attcctcaat tagaacttct cttacataga tgactttcta 94500 agcattggaa agaaaccata gtcagaaact agaaaaaaga gatattcact aaccaattgg 94560 gtcatgctgc tgcacaaaga aattgtctcc ctcctctggc ttcagcatgg attctgtttt 94620 tctttgcatt ttttgtaaaa tggaaagtag tcaattagcc accaaattca gcatcctgaa 94680 attgcttttt tgcaagaaga taactttgca acccatgggt tagagaaaca accttagatt 94740 ttagatttta gattttagaa gtttagattt taaaactgac tccaccactt tttacttgtg 94800 ttaccttgga gattgctaaa catttctgaa gctcattttt attattgctg atatggtgac 94860 agtatttgtc tttcaatcct aagcagtgac tgttgggttt aaggaatatt accagtgctg 94920 ggcacatggg tgtattcctt catttttttt atccattcag taagcatttg tgaagtgtct 94980 gcaggctccc aggtactgtg caaggtatcg aggacacaaa gtcaaataat ggtcaaatcc 95040 ctagatattc cttgtttcct ttctttaagt tattgttaaa gtaaatgttg tggaaaacat 95 100 atttttgtgg cacctaagag agccttttct gatttgatct gcctgtaagt ggacactagt 95160 cctgctttga tttttctaat aaatttcttg gaattacttc tctccctctg gaactttgga 95220 acttgaaaga acagtataat tagcagctac cactatttaa aatgttaaca acacaatttg 95280 attctaaaaa ttcttacaaa taaactgtct atattgatgt tttcagactc taaggccagt 95340 attattaccc tttccacagg ggaaatgtgc tcggtgagaa aaactggcca ctatttctgc 95400 attttctagt ttcatgttct tctgtacaat attgccatcc ttcttgattg tgttttgttg 95460 gtttgttttg gtttttattt taaagccaaa agaaaaaaaa ttaagctgcc tcctatgtaa 95520 aatttcttaa ggatattgtg tatctttata aaatatttgg attgactttt tgaaaatcaa 95580 aattacatca gtatgaaaat taataatcag aaaaaagtaa ttgttcttta taacttttat 95640 ttttagccca gttgcaaagt aacagcaatg aagtgctttc tcttggagtt acaagttatt 95700 tcacttgagt ccggagatgc aagtattcat gatacagtag aaaatctgat catcctagca 95760 acaaagaggc ataatcaaat tagttcattt cataaatact ttttagccct caggagacag 95820 tatgaaaaaa tacccataat ccctgctgtt aaagagggag gaaacagaaa taaaccaagc 95880 actcagcaaa tgagtaataa gttagttatt tatgaagtcc agtggtgaaa aaggacacag 95940 ggtaggggca ctggggctcc agttagggta ggctgagcta ataagctgcc atttgacaaa 96000 gatgtgagag aatgagcctt aagggtatct agggacatat gtttaggcag agacaactga 96060 aagtgcaaag gccctaagct tgtgtgggtg gacaccttga cttttctcta aaaatacaag 96120 actgctttag gtctttgctt cagtgtcacc ttctcagtga tttacctatt tacagttgca 96180 atcccttccc tatctgagca ctccctgtcc ccttctctgc gtgtcccata tctggaatat 96240 gatatatttt atgtaatggg tattgtctgt ttttccctac tagaatgtca gcttaaaatg 96300 gacaagtgtt tttgtttttt attagtacta tatccaaatt cccagaacag tgtctcatat 96360 atattaaata ctttacatat gtatgaaatg aatttattaa ggtttataat actcttatga 96420 tgttatctcc atcttattgg tggaaagagt gagccacgca acaggtaact catggcaaaa 96480 taaatgatga gctctttaat gtgaatgttg atagtcatcc tcatcatgac agagtgtgac 96540 atacgtgctg gaatgcgtgg gcaggactgc acagtcagaa gacctggatg ggaagaattt 96600 cagttgatta tgtcagttga gatgagtcaa tatctctgtc ttcctatctg acatgattat 96660 tttcaggatc aaataggaca gcattatatg aaatatgaca gtgcttggca tagaggatgt 96720 gcttgataaa tgtcgctgat tctgaattcc aaatagttaa tggatataaa atctaatttt 96780 tatttttgct ggcacagcct tcatggttca gatgatctct aaaaagttta ttcttaattg 96840 ctacatttgg aacattctag ttatccatga gactgtgttt gaagtacaac aatttgtggt 96900 attcacctaa atggctcagt tcaaccaatg ggtgattaca gtgtgtcagg ccctgtgcta 96960 aatgctaggg aaacaaacat actggctctg accttctggc agagagctta gagaatagta 97020 ttacagtata aaaagagtaa aacttcataa tatgaataga atacatgaca tattgtgaga 97080 gaggtctaaa taaggggttc tgagatgtaa agatgggaca ttgagtccag ccctaggagc 97140 ccaggggttc agagaagcct ccctaaaaga gtacctgaat aatcttaagt gcgagtagca 97200 gcagctaaaa taggaatgaa tgtaacagag agaaaagcaa aggcagagag gcatctaata 97260 gtatgctgtg catggcaagg aaagccagat gctcactcag tgtttctagg gcataacatt 97320 gaggcaaggg ggatatgtga aataaatagg taaggaacag ctcatgaaga gcctgtgtgc 97380 tatgcaggga ggcatggtct ttatgctaga agcagatgat aggaaaccat tgaagggctc 97440 aaagcaggaa ctccgtggtc tgatagacac agcttatttt tctcaatgtc cttaacccat 97500 tattggaaaa ataaacatat atcaaatctc cctgttctct ttcctcgtag ttcttcctaa 97560 tatgctattt atttgctaaa gctttcatat ctcaagacct caccatatgg ttcaaacgat 97620 atgatattcc cattcccatg aatgtatatt taatttaatt ataaattgcc aatttaatct 97680 ctctctatat tttgcagaat gtaacagaat ctggatgcaa agaatgtgag gaactggagg 97740 aaaaaaatat taaagaattt ttgcagagtt ttgtacatat tgtccaaatg ttcatcaaca 97800 cttcttgatt gcaattgatt ctttttaaag tgtttctgtt attaacaaac atcactctgc 97860 tgcttagaca taacaaaaca ctcggcattt caaatgtgct gtcaaaacaa gtttttctgt 97920 caagaagatg atcagacctt ggatcagatg aactcttaga aatgaaggca gaaaaatgtc 97980 attgagtaat atagtgacta tgaacttctc tcagacttac tttactcatt tttttaattt 98040 attattgaaa ttgtacatat ttgtggaata atgtaaaatg ttgaataaaa atatgtacaa 98 100 gtgttgtttt ttaagttgca ctgatatttt acctcttatt gcaaaatagc atttgtttaa 98160 gggtgatagt caaattatgt attggtgggg ctgggtacca atgctgcagg tcaacagcta 98220 tgctggtagg ctcctgccag tgtggaacca ctgactactg gctctcattg acttccttac 98280 taagcatagc aaacagagga agaatttgtt atcagtaaga aaaagaagaa ctatatgtga 98340 atcctcttct ttatactgta atttagttat tgatgtataa agcaactgtt atgaaataaa 98400 gaaattgcaa taactggcat ataatgtcca tcagtaaatc ttggtggtgg tggcaataat 98460 aaacttctac tgataggtag aatggtgtgc aagcttgtcc aatcacggat tgcaggccac 98520 atgcggccca ggacaacttt gaatgtggcc caacacaaat tcataaactt tcatacatct 98580 cgtttttagc tcatcagcta tcattagcgg tagtgtattt aaagtgtggc ccaagacaat 98640 tcttcttatt ccaatgtggc ccagggaaat caaaagattg gatgcccctg gtatagaaaa 98700 ctaatagtga cagtgttcat atttcatgct ttcccaaata caggtatttt attttcacat 98760 tctttttgcc atgtttatat aataataaag aaaaaccctg ttgatttgtt ggagccatt 98819  

Claims (11)

Selected from within the interleukin ( IL ) -15 genomic gene having SEQ ID NO: 15, the +20 base is G from the translation start point (85265 base of SEQ ID NO: 15) and comprises the +20 base Polymorphic marker for osteoporosis prediction consisting of polynucleotide consisting of 20 to 100 consecutive nucleic acid molecules. 20 to 100 consecutive nucleic acids selected from the IL-15 genomic gene having SEQ ID NO: 15, wherein the +13467 base is A from the translation start point (85265 base of SEQ ID NO: 15) and includes the +13467 base Polymorphic marker for predicting osteoporosis composed of polynucleotide consisting of molecules. 20-100 consecutive nucleic acids selected from the IL-15 genomic gene having SEQ ID NO: 15, wherein the +13653 th base is T from the translation start point (85265 base of SEQ ID NO: 15) and includes the +13653 th base Polynucleotides associated with osteoporosis composed of molecules. 20-100 consecutive nucleic acids selected from the IL-15 genomic gene having SEQ ID NO: 15, wherein the +13653 th base is T from the translation start point (85265 base of SEQ ID NO: 15) and includes the +13653 th base Polymorphic marker for predicting osteoporosis composed of polynucleotide consisting of molecules. 20 to 100 consecutive nucleic acids selected from the IL-15 genomic gene having SEQ ID NO: 15, wherein the +13815 base is T from the translation start point (85265 base of SEQ ID NO: 15) and comprises the +13815 base Polymorphic marker for predicting osteoporosis composed of polynucleotide consisting of molecules. Selected from the IL-15 genomic gene having SEQ ID NO: 15, from the translation start point (85265 base in SEQ ID NO: 15), the +20 base is G, the +13467 base is C, the +13653 base is A, +13815 Haplotype marker for predicting osteoporosis wherein the first base is T. Selected from the IL-15 genomic gene having SEQ ID NO: 15, the +20 th base is A, the +13467 th base A, the +13653 th base T, and +13815 from the translation start point (85265 base of SEQ ID NO: 15) Haplotype marker for predicting osteoporosis with the first base A. 1) extracting genomic DNA from blood samples isolated from the subject; 2) detecting the polymorphic marker for predicting osteoporosis of any one of claims 1, 2, 4 or 5 present on the genomic DNA of step 1); And, 3) providing information for predicting osteoporosis comprising comparing the detection results of step 2) with the results of detection of polymorphic sites in normal individuals. The method of claim 8, wherein step 2) comprises sequencing analysis, hybridization by microarray, allele specific PCR, dynamic allele-specific hybridization (DASH), and PCR extension. Characterized in that performed by analysis or TaqMan technique. A kit for predicting osteoporosis comprising a primer pair capable of amplifying a polymorphic marker for predicting osteoporosis according to any one of claims 1, 2, 4 or 5. The kit for predicting osteoporosis according to claim 10, wherein the primer pair is selected from the group consisting of the following primer pairs 1 to 3. Primer pair 1: forward primer set forth in SEQ ID NO: 1 and reverse primer set forth in SEQ ID NO: 2; Primer pair 2: forward primer set forth in SEQ ID NO: 3 and reverse primer set forth in SEQ ID NO: 4; And, Primer pair 3: forward primer set forth in SEQ ID NO: 5 and reverse primer set forth in SEQ ID NO: 6.

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